DAMIAN KRYSAN

Summary

Affiliation: University of Rochester
Country: USA

Publications

  1. pmc A large-scale complex haploinsufficiency-based genetic interaction screen in Candida albicans: analysis of the RAM network during morphogenesis
    Nike Bharucha
    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan, United States of America
    PLoS Genet 7:e1002058. 2011
  2. ncbi request reprint A high-throughput screening assay for small molecules that disrupt yeast cell integrity
    Damian J Krysan
    Department of Pediatrics, Division of Pediatric Infectious Diseases, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA
    J Biomol Screen 13:657-64. 2008
  3. pmc Live Candida albicans suppresses production of reactive oxygen species in phagocytes
    Melanie Wellington
    Department of Pediatrics, University of Rochester School of Medicine and Dentistry, 601 Elmwood Ave, Box 690, Rochester, NY 14642, USA
    Infect Immun 77:405-13. 2009
  4. pmc Yapsins are a family of aspartyl proteases required for cell wall integrity in Saccharomyces cerevisiae
    Damian J Krysan
    Division of Pediatric Infectious Disease, Department of Pediatrics and Communicable Diseases, University of Michigan Hospitals, Ann Arbor, Michigan 48109, USA
    Eukaryot Cell 4:1364-74. 2005
  5. pmc The unfolded protein response is induced by the cell wall integrity mitogen-activated protein kinase signaling cascade and is required for cell wall integrity in Saccharomyces cerevisiae
    Thomas Scrimale
    Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Mol Biol Cell 20:164-75. 2009
  6. ncbi request reprint A high-throughput assay of yeast cell lysis for drug discovery and genetic analysis
    Louis Didone
    Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA
    Nat Protoc 5:1107-14. 2010
  7. pmc Adenylate kinase release as a high-throughput-screening-compatible reporter of bacterial lysis for identification of antibacterial agents
    Anna C Jacobs
    Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York, USA
    Antimicrob Agents Chemother 57:26-36. 2013
  8. pmc Antifungal activity of tamoxifen: in vitro and in vivo activities and mechanistic characterization
    Kristy Dolan
    Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Antimicrob Agents Chemother 53:3337-46. 2009
  9. pmc Cleavage of the signaling mucin Msb2 by the aspartyl protease Yps1 is required for MAPK activation in yeast
    Nadia Vadaie
    Department of Biological Sciences, State University of New York at Buffalo, Buffalo, NY 14260, USA
    J Cell Biol 181:1073-81. 2008
  10. pmc Unconventional genomic architecture in the budding yeast saccharomyces cerevisiae masks the nested antisense gene NAG1
    Jun Ma
    Department of Molecular, Cellular, and Developmental Biology, Life Sciences Institute, Room 6026, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, MI 48109 2216, USA
    Eukaryot Cell 7:1289-98. 2008

Collaborators

  • Melanie Wellington
  • Paul M Dunman
  • Anuj Kumar
  • Paul J Cullen
  • Tao Xu
  • C Abeijon
  • Louis Didone
  • Thomas Scrimale
  • Anna C Jacobs
  • Nike Bharucha
  • Craig J Dobry
  • Kristy Dolan
  • Nathan C Rockwell
  • Jun Ma
  • Nadia Vadaie
  • Laura Rozan
  • Robert S Fuller
  • Jennielle Jobson
  • Madeline K Sofia
  • Sarah Sobczynski
  • Cole Johnson
  • Yeissa Chabrier-Rosell√≥
  • Christopher P Anderson
  • Andrew J Benjamin
  • Qingxuan Song
  • Matthew J Eckwahl
  • Bonnie K Baxter
  • Sara Montgomery
  • Bradley Buchheit
  • Karen L de Mesy Bentley
  • Seth R Nickerson
  • Heather Dionne
  • Darowan S Akajagbor
  • Tomoko Komiyama

Detail Information

Publications14

  1. pmc A large-scale complex haploinsufficiency-based genetic interaction screen in Candida albicans: analysis of the RAM network during morphogenesis
    Nike Bharucha
    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan, United States of America
    PLoS Genet 7:e1002058. 2011
    ..albicans and support a model in which these two pathways regulate a common set of genes at different stages of filamentation...
  2. ncbi request reprint A high-throughput screening assay for small molecules that disrupt yeast cell integrity
    Damian J Krysan
    Department of Pediatrics, Division of Pediatric Infectious Diseases, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA
    J Biomol Screen 13:657-64. 2008
    ..Fungistatic compounds are not detected by the assay. Adenylate kinase-based screening appears to be a useful approach to the direct identification of small molecules that kill yeast cells...
  3. pmc Live Candida albicans suppresses production of reactive oxygen species in phagocytes
    Melanie Wellington
    Department of Pediatrics, University of Rochester School of Medicine and Dentistry, 601 Elmwood Ave, Box 690, Rochester, NY 14642, USA
    Infect Immun 77:405-13. 2009
    ..These studies indicate that live C. albicans actively suppresses ROS production in phagocytes in vitro, which may represent an important immune evasion mechanism...
  4. pmc Yapsins are a family of aspartyl proteases required for cell wall integrity in Saccharomyces cerevisiae
    Damian J Krysan
    Division of Pediatric Infectious Disease, Department of Pediatrics and Communicable Diseases, University of Michigan Hospitals, Ann Arbor, Michigan 48109, USA
    Eukaryot Cell 4:1364-74. 2005
    ..Taken together, these data indicate that the yapsins play an important role in glucan homeostasis in S. cerevisiae and that yapsin homologues may play a similar role in the pathogenic yeasts C. albicans and C. glabrata...
  5. pmc The unfolded protein response is induced by the cell wall integrity mitogen-activated protein kinase signaling cascade and is required for cell wall integrity in Saccharomyces cerevisiae
    Thomas Scrimale
    Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Mol Biol Cell 20:164-75. 2009
    ..We propose that the cellular responses to ER and cell wall stress are coordinated to buffer the cell against these two related cellular stresses...
  6. ncbi request reprint A high-throughput assay of yeast cell lysis for drug discovery and genetic analysis
    Louis Didone
    Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA
    Nat Protoc 5:1107-14. 2010
    ..75-0.9), making it a highly robust assay. The AK assay is performed in a single microtiter plate using an 'add and read' procedure that can be completed in a single work day...
  7. pmc Adenylate kinase release as a high-throughput-screening-compatible reporter of bacterial lysis for identification of antibacterial agents
    Anna C Jacobs
    Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York, USA
    Antimicrob Agents Chemother 57:26-36. 2013
    ..Our data demonstrate that the AK assay provides an attractive screening approach for identifying new antimicrobial agents. Further, terfenadine and tamoxifen may represent novel antimicrobial drug development scaffolds...
  8. pmc Antifungal activity of tamoxifen: in vitro and in vivo activities and mechanistic characterization
    Kristy Dolan
    Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Antimicrob Agents Chemother 53:3337-46. 2009
    ..Taken together, these experiments indicate that the further study of compounds related to TAM as antifungal agents is warranted...
  9. pmc Cleavage of the signaling mucin Msb2 by the aspartyl protease Yps1 is required for MAPK activation in yeast
    Nadia Vadaie
    Department of Biological Sciences, State University of New York at Buffalo, Buffalo, NY 14260, USA
    J Cell Biol 181:1073-81. 2008
    ..We postulate that cleavage-dependent activation is a general feature of signaling mucins, which brings to light a novel regulatory aspect of this class of signaling adhesion molecule...
  10. pmc Unconventional genomic architecture in the budding yeast saccharomyces cerevisiae masks the nested antisense gene NAG1
    Jun Ma
    Department of Molecular, Cellular, and Developmental Biology, Life Sciences Institute, Room 6026, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, MI 48109 2216, USA
    Eukaryot Cell 7:1289-98. 2008
    ..Thus, NAG1 is important for two reasons. First, it contributes to yeast cell wall biogenesis. Second, its genomic context is novel, raising the possibility that other nested protein-coding genes may exist in eukaryotic genomes...
  11. ncbi request reprint Plasticity of extended subsites facilitates divergent substrate recognition by Kex2 and furin
    Laura Rozan
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Biol Chem 279:35656-63. 2004
    ..E255I-Kex2 exhibited significantly decreased recognition of P(4) Arg in a tetrapeptide substrate with Lys at P(1), although the general pattern of selectivity for aliphatic residues at P(4) remained unchanged...
  12. ncbi request reprint Precursor processing by kex2/furin proteases
    Nathan C Rockwell
    Department of Biological Chemistry, University of Michigan Medical Center, Room 5413 Med Sci I, 1301 East Catherine, Ann Arbor Michigan 48109, USA
    Chem Rev 102:4525-48. 2002

Research Grants4

  1. Yeast Aspartyl Proteases in Cell Wall Integrity
    DAMIAN KRYSAN; Fiscal Year: 2007
    ..A team of outstanding collaborators and advisors has been assembled to provide additional mentoring and support as the investigator makes the transition to an independent researcher. ..
  2. Chemical Genetic HTS Assay for Inhibitors of Fungal Cell Wall Integrity
    DAMIAN KRYSAN; Fiscal Year: 2009
    ....
  3. Chemical Genetic HTS Assay for Inhibitors of Fungal Cell Wall Integrity
    Damian J Krysan; Fiscal Year: 2010
    ....
  4. Discovery and evaluation of fungicidal anti-cryptococcal molecules
    Damian J Krysan; Fiscal Year: 2010
    ..If toremifene is active in this model, it could be rapidly moved to clinical trials as a fungicidal drug that is taken by mouth and, thus, useful in resource-limited regions. ..