CLAUDE F KRUMMENACHER

Summary

Affiliation: University of Pennsylvania
Country: USA

Publications

  1. pmc Herpes simplex virus glycoprotein D can bind to poliovirus receptor-related protein 1 or herpesvirus entry mediator, two structurally unrelated mediators of virus entry
    C Krummenacher
    Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Virol 72:7064-74. 1998
  2. ncbi Comparative usage of herpesvirus entry mediator A and nectin-1 by laboratory strains and clinical isolates of herpes simplex virus
    Claude Krummenacher
    Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Virology 322:286-99. 2004
  3. pmc Cellular localization of nectin-1 and glycoprotein D during herpes simplex virus infection
    Claude Krummenacher
    Department of Microbiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6002, USA
    J Virol 77:8985-99. 2003
  4. pmc Effects of herpes simplex virus on structure and function of nectin-1/HveC
    Claude Krummenacher
    Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Virol 76:2424-33. 2002
  5. pmc Localization of a binding site for herpes simplex virus glycoprotein D on herpesvirus entry mediator C by using antireceptor monoclonal antibodies
    C Krummenacher
    Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Virol 74:10863-72. 2000
  6. pmc The first immunoglobulin-like domain of HveC is sufficient to bind herpes simplex virus gD with full affinity, while the third domain is involved in oligomerization of HveC
    C Krummenacher
    Department of Microbiology, School of Dental Medicine, Philadelphia, Pennsylvania 19104, USA
    J Virol 73:8127-37. 1999
  7. pmc The herpes simplex virus receptor nectin-1 is down-regulated after trans-interaction with glycoprotein D
    Katie M Stiles
    Department of Microbiology, School of Dental Medicine University of Pennsylvania, Philadelphia, PA 19104, USA
    Virology 373:98-111. 2008
  8. pmc Structure of unliganded HSV gD reveals a mechanism for receptor-mediated activation of virus entry
    Claude Krummenacher
    Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA
    EMBO J 24:4144-53. 2005
  9. pmc Engineered disulfide bonds in herpes simplex virus type 1 gD separate receptor binding from fusion initiation and viral entry
    Eric Lazear
    Department of Microbiology, University of Pennsylvania, 240 S 40th St, Levy Rm 233, Philadelphia, PA 19104, USA
    J Virol 82:700-9. 2008
  10. pmc Glycoprotein D actively induces rapid internalization of two nectin-1 isoforms during herpes simplex virus entry
    Katie M Stiles
    Department of Microbiology, School of Dental Medicine University of Pennsylvania, Philadelphia, PA 19104, USA
    Virology 399:109-19. 2010

Research Grants

Collaborators

Detail Information

Publications12

  1. pmc Herpes simplex virus glycoprotein D can bind to poliovirus receptor-related protein 1 or herpesvirus entry mediator, two structurally unrelated mediators of virus entry
    C Krummenacher
    Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Virol 72:7064-74. 1998
    ..Thus, we propose that HSV entry can be mediated by two structurally unrelated gD receptors through related but not identical binding with gD...
  2. ncbi Comparative usage of herpesvirus entry mediator A and nectin-1 by laboratory strains and clinical isolates of herpes simplex virus
    Claude Krummenacher
    Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Virology 322:286-99. 2004
    ..Overall, our data suggest that both receptors play a role during HSV infection in vivo and that both are highly efficient even at low levels of expression...
  3. pmc Cellular localization of nectin-1 and glycoprotein D during herpes simplex virus infection
    Claude Krummenacher
    Department of Microbiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6002, USA
    J Virol 77:8985-99. 2003
    ..We propose that gD attracts and maintains the receptor at junctions where it can be used for virus spread...
  4. pmc Effects of herpes simplex virus on structure and function of nectin-1/HveC
    Claude Krummenacher
    Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Virol 76:2424-33. 2002
    ..These observations suggest that cell surface nectin-1 and gD may undergo conformational changes during HSV entry as part of an evolving interaction between the viral envelope and the cell plasma membrane...
  5. pmc Localization of a binding site for herpes simplex virus glycoprotein D on herpesvirus entry mediator C by using antireceptor monoclonal antibodies
    C Krummenacher
    Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Virol 74:10863-72. 2000
    ..302 (conformational epitopes) to HveC and vice versa. Epitope mapping showed that CK6 and CK8 bound between residues 80 and 104 of HveC, suggesting that part of the gD binding site colocalizes in the same region...
  6. pmc The first immunoglobulin-like domain of HveC is sufficient to bind herpes simplex virus gD with full affinity, while the third domain is involved in oligomerization of HveC
    C Krummenacher
    Department of Microbiology, School of Dental Medicine, Philadelphia, Pennsylvania 19104, USA
    J Virol 73:8127-37. 1999
    ..In addition, the stoichiometry between gD and HveC appeared to be influenced by the level of HveC oligomerization...
  7. pmc The herpes simplex virus receptor nectin-1 is down-regulated after trans-interaction with glycoprotein D
    Katie M Stiles
    Department of Microbiology, School of Dental Medicine University of Pennsylvania, Philadelphia, PA 19104, USA
    Virology 373:98-111. 2008
    ..Moreover, gD is necessary for virion internalization in B78H1 cells expressing nectin-1. These data suggest that the determinants of gD-mediated internalization of nectin-1 may direct HSV to an endocytic pathway during entry...
  8. pmc Structure of unliganded HSV gD reveals a mechanism for receptor-mediated activation of virus entry
    Claude Krummenacher
    Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA
    EMBO J 24:4144-53. 2005
    ..We propose that a controlled displacement of the gD C-terminus upon receptor binding is an essential feature of HSV entry, ensuring the timely activation of membrane fusion...
  9. pmc Engineered disulfide bonds in herpes simplex virus type 1 gD separate receptor binding from fusion initiation and viral entry
    Eric Lazear
    Department of Microbiology, University of Pennsylvania, 240 S 40th St, Levy Rm 233, Philadelphia, PA 19104, USA
    J Virol 82:700-9. 2008
    ..Based upon the properties of the panel of mutants we conclude that fusion requires greater flexibility of the gD ectodomain C terminus than does receptor binding...
  10. pmc Glycoprotein D actively induces rapid internalization of two nectin-1 isoforms during herpes simplex virus entry
    Katie M Stiles
    Department of Microbiology, School of Dental Medicine University of Pennsylvania, Philadelphia, PA 19104, USA
    Virology 399:109-19. 2010
    ..Overall, this study shows that gD binding actively induces the rapid internalization of various forms of nectin-1. We suggest that HSV activates a nectin-1 internalization pathway to use for endocytic entry...
  11. pmc Entry of herpes simplex virus type 1 into primary sensory neurons in vitro is mediated by Nectin-1/HveC
    Sarah M Richart
    Department of Microbiology, Colorado State University, Fort Collins, Colorado, USA
    J Virol 77:3307-11. 2003
    ..Soluble, truncated nectin-1 but not HveA prevented viral entry. Antibodies against nectin-1 also blocked infection of rat neurons. These results indicate that nectin-1 is the primary receptor for HSV-1 infection of sensory neurons...
  12. pmc Herpes simplex virus glycoprotein D interferes with binding of herpesvirus entry mediator to its ligands through downregulation and direct competition
    Katie M Stiles
    Department of Microbiology, University of Pennsylvania, Philadelphia, PA, USA
    J Virol 84:11646-60. 2010
    ..Thus, gD is at the center of the interplay between HVEM and its ligands. It can interfere with HVEM function in two ways, by competing with the natural ligands and by downregulating HVEM from the cell surface...

Research Grants2

  1. Interactions of herpes simplex virus with nectin-1 at cell junctions
    Claude Krummenacher; Fiscal Year: 2007
    ..By identifying new requirements for virus entry in the host, this study will provide targets for novel therapeutic approaches to interfere with this process. ..