Stephen J Kron

Summary

Affiliation: University of Chicago
Country: USA

Publications

  1. ncbi request reprint Filamentous growth in budding yeast
    S J Kron
    Center for Molecular Oncology, University of Chicago, Chicago, IL 60637, USA
    Trends Microbiol 5:450-4. 1997
  2. pmc Yeast G1 DNA damage checkpoint regulation by H2A phosphorylation is independent of chromatin remodeling
    Ali Javaheri
    Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 103:13771-6. 2006
  3. pmc Role of Dot1-dependent histone H3 methylation in G1 and S phase DNA damage checkpoint functions of Rad9
    Robert Wysocki
    Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA
    Mol Cell Biol 25:8430-43. 2005
  4. pmc Response of human prostate cancer cells and tumors to combining PARP inhibition with ionizing radiation
    Juan Camilo Barreto-Andrade
    Ludwig Center for Metastasis Research, The University of Chicago, 5841 South Maryland Avenue, MC 9006, Chicago, IL 60637, USA
    Mol Cancer Ther 10:1185-93. 2011
  5. ncbi request reprint Non-catalytic function for ATR in the checkpoint response
    Troy D McSherry
    Center for Molecular Oncology, University of Chicago, Chicago, Illinois, USA
    Cell Cycle 6:2019-30. 2007
  6. pmc Peptide reporters of kinase activity in whole cell lysates
    Ding Wu
    Ludwig Center for Metastasis Research, The University of Chicago, Chicago, IL 60637, USA
    Biopolymers 94:475-86. 2010
  7. pmc A pairwise chemical genetic screen identifies new inhibitors of glucose transport
    Olesya A Ulanovskaya
    Department of Chemistry, The University of Chicago, Chicago, IL 60637, USA
    Chem Biol 18:222-30. 2011
  8. pmc A magnetic bead-based protein kinase assay with dual detection techniques
    Guangchang Zhou
    Ludwig Center for Metastasis Research, The University of Chicago, IL 60637, USA
    Anal Biochem 408:5-11. 2011
  9. pmc Dissection of Rad9 BRCT domain function in the mitotic checkpoint response to telomere uncapping
    CHINONYE C NNAKWE
    Department of Pathology, The University of Chicago, Chicago, IL 60637, USA
    DNA Repair (Amst) 8:1452-61. 2009
  10. doi request reprint Photocleavable peptide-conjugated magnetic beads for protein kinase assays by MALDI-TOF MS
    Guangchang Zhou
    Ludwig Center for Metastasis Research, The University of Chicago, Chicago, Illinois 60637, USA
    Bioconjug Chem 21:1917-24. 2010

Collaborators

Detail Information

Publications43

  1. ncbi request reprint Filamentous growth in budding yeast
    S J Kron
    Center for Molecular Oncology, University of Chicago, Chicago, IL 60637, USA
    Trends Microbiol 5:450-4. 1997
    ..The puzzle of why most strains are nonfilamentous is now resolved. Remarkably, a single point mutation with broad consequences separates these domesticated yeast from their wild ancestors...
  2. pmc Yeast G1 DNA damage checkpoint regulation by H2A phosphorylation is independent of chromatin remodeling
    Ali Javaheri
    Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 103:13771-6. 2006
    ....
  3. pmc Role of Dot1-dependent histone H3 methylation in G1 and S phase DNA damage checkpoint functions of Rad9
    Robert Wysocki
    Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA
    Mol Cell Biol 25:8430-43. 2005
    ..These results indicate a key role for chromatin and methylation of histone H3 Lys 79 in yeast DNA damage signaling...
  4. pmc Response of human prostate cancer cells and tumors to combining PARP inhibition with ionizing radiation
    Juan Camilo Barreto-Andrade
    Ludwig Center for Metastasis Research, The University of Chicago, 5841 South Maryland Avenue, MC 9006, Chicago, IL 60637, USA
    Mol Cancer Ther 10:1185-93. 2011
    ....
  5. ncbi request reprint Non-catalytic function for ATR in the checkpoint response
    Troy D McSherry
    Center for Molecular Oncology, University of Chicago, Chicago, Illinois, USA
    Cell Cycle 6:2019-30. 2007
    ..Thus, the link between the non-catalytic region of the ATR kinase family and the spindle checkpoint pathway is conserved...
  6. pmc Peptide reporters of kinase activity in whole cell lysates
    Ding Wu
    Ludwig Center for Metastasis Research, The University of Chicago, Chicago, IL 60637, USA
    Biopolymers 94:475-86. 2010
    ..These strategies can be generalized to improve the reactivity of most peptide substrates used in heterogeneous kinase assays with cell lysates...
  7. pmc A pairwise chemical genetic screen identifies new inhibitors of glucose transport
    Olesya A Ulanovskaya
    Department of Chemistry, The University of Chicago, Chicago, IL 60637, USA
    Chem Biol 18:222-30. 2011
    ....
  8. pmc A magnetic bead-based protein kinase assay with dual detection techniques
    Guangchang Zhou
    Ludwig Center for Metastasis Research, The University of Chicago, IL 60637, USA
    Anal Biochem 408:5-11. 2011
    ..Formatted for 96-well plates and with high-throughput potential, this dual detection kinase assay may provide a rapid, reliable, and inexpensive route to the discovery of small-molecule drug leads...
  9. pmc Dissection of Rad9 BRCT domain function in the mitotic checkpoint response to telomere uncapping
    CHINONYE C NNAKWE
    Department of Pathology, The University of Chicago, Chicago, IL 60637, USA
    DNA Repair (Amst) 8:1452-61. 2009
    ....
  10. doi request reprint Photocleavable peptide-conjugated magnetic beads for protein kinase assays by MALDI-TOF MS
    Guangchang Zhou
    Ludwig Center for Metastasis Research, The University of Chicago, Chicago, Illinois 60637, USA
    Bioconjug Chem 21:1917-24. 2010
    ....
  11. pmc DNA resection proteins Sgs1 and Exo1 are required for G1 checkpoint activation in budding yeast
    Fiyinfolu O Balogun
    Committee on Molecular Pathogenesis and Molecular Medicine, University of Chicago, Chicago, IL 60637, USA
    DNA Repair (Amst) 12:751-60. 2013
    ..Together, these results implicate DNA resection, which is thought to be minimal in G1, as necessary for activation of the G1 checkpoint. ..
  12. pmc Radiation-inducible immunotherapy for cancer: senescent tumor cells as a cancer vaccine
    Yuru Meng
    Ludwig Center for Metastasis Research, The University of Chicago, Chicago, Illinois, USA
    Mol Ther 20:1046-55. 2012
    ..Applied to human cancers, radiation-inducible immunotherapy may enhance radiotherapy responses to prevent local recurrence and distant metastasis...
  13. pmc CDK-dependent Hsp70 Phosphorylation controls G1 cyclin abundance and cell-cycle progression
    Andrew W Truman
    Ludwig Center for Metastasis Research, The University of Chicago, Chicago, IL 60637, USA
    Cell 151:1308-18. 2012
    ..These results establish an active role for Hsp70 chaperones as signal transducers mediating growth control of G1 cyclin abundance and activity...
  14. pmc Photocleavable peptide-oligonucleotide conjugates for protein kinase assays by MALDI-TOF MS
    Guangchang Zhou
    Department of Molecular Genetics and Cell Biology, Gordon Center for Integrative Sciences, W522A, The University of Chicago, Chicago, IL 60637, USA
    Mol Biosyst 8:2395-404. 2012
    ..This new assay enables analysis of protein kinase activities in a multiplexed format amenable to screening inhibitors against multiple kinases in parallel, an important capability for drug discovery and predictive diagnostics...
  15. pmc A solid-phase Bcr-Abl kinase assay in 96-well hydrogel plates
    Ding Wu
    Ludwig Center for Metastasis Research, University of Chicago, Chicago, IL 60637, USA
    Anal Biochem 375:18-26. 2008
    ..This assay provides a practical method to measure the activity of a single kinase present in a whole cell lysate with high sensitivity and specificity as a valuable means for efficient small molecule screening...
  16. pmc A bead-based activity screen for small-molecule inhibitors of signal transduction in chronic myelogenous leukemia cells
    Juliesta E Sylvester
    Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, USA
    Mol Cancer Ther 9:1469-81. 2010
    ..This method for analyzing a focused signaling network benefits from rigorous statistical analysis and short processing times, thereby offering a powerful tool for drug discovery and clinical testing...
  17. ncbi request reprint Photocleavable peptide hydrogel arrays for MALDI-TOF analysis of kinase activity
    Laurie L Parker
    Department of Biochemistry and Molecular Biology, University of Chicago, CIS 201, 929 E 57th Street, Chicago, IL 60637, USA
    Analyst 131:1097-104. 2006
    ..This work serves as proof of principle for modular substrate design strategies for mass spectrometry-readable biosensors...
  18. pmc Rapid validation of Mascot search results via stable isotope labeling, pair picking, and deconvolution of fragmentation patterns
    Samuel L Volchenboum
    Department of Pediatrics, The University of Chicago, Chicago, Illinois 60637, USA
    Mol Cell Proteomics 8:2011-22. 2009
    ..DAT file in less than 6 min (32 spectra/s), revealing high confidence peptide identifications without regard to Mascot score, far faster than manual or other independent validation methods...
  19. pmc Phosphoprotein profiling by PA-GeLC-MS/MS
    Kolbrun Kristjansdottir
    Department of Molecular Genetics and Cell Biology, and Ludwig Center for Metastasis Research, The University of Chicago, Chicago, Illinois 60637, USA
    J Proteome Res 7:2812-24. 2008
    ....
  20. pmc Control of the yeast cell cycle with a photocleavable alpha-factor analogue
    Laurie L Parker
    Department of Biochemistry and Molecular Biology, University of Chicago, 929 E 57th Street, CIS W201A, Chicago, IL 60637, USA
    Angew Chem Int Ed Engl 45:6322-5. 2006
  21. ncbi request reprint Relevance and irrelevance of DNA damage response to radiotherapy
    Philip P Connell
    Department of Radiation and Cellular Oncology, Center for Molecular Oncology, University of Chicago, 5759 S Maryland Avenue, MC 9006, Chicago, IL 60637, USA
    DNA Repair (Amst) 3:1245-51. 2004
    ..Our goal is to frame the issues that contribute to the apparent disconnect between laboratory discoveries and improvements in clinically relevant therapeutics...
  22. pmc NuA4 subunit Yng2 function in intra-S-phase DNA damage response
    John S Choy
    Department of Molecular Genetics and Cell Biology Center for Molecular Oncology, University of Chicago, Chicago, Illinois 60637, USA
    Mol Cell Biol 22:8215-25. 2002
    ..Our results implicate nucleosomal histone acetylation in maintaining genomic integrity during chromosomal replication...
  23. pmc Yeast cell death during DNA damage arrest is independent of caspase or reactive oxygen species
    Robert Wysocki
    Center for Molecular Oncology, The University of Chicago, 924 E 57th Street, Rm R320, IL 60637, USA
    J Cell Biol 166:311-6. 2004
    ..We speculate that during a prolonged cell cycle arrest, cdc13-1 cells reach a critical size and die by cell lysis...
  24. pmc Investigating quantitation of phosphorylation using MALDI-TOF mass spectrometry
    Laurie Parker
    Ludwig Center for Metastasis Research, University of Chicago, Knapp R322, 924 E 57th Street, Chicago, IL 60637, USA
    J Mass Spectrom 43:518-27. 2008
    ....
  25. ncbi request reprint Assaying Bcr-Abl kinase activity and inhibition in whole cell extracts by phosphorylation of substrates immobilized on agarose beads
    Ding Wu
    Center for Molecular Oncology and Department of Molecular Genetics and Cell Biology, University of Chicago, IL 60637, USA
    Anal Biochem 347:67-76. 2005
    ..This simple kinase assay is sufficiently straightforward and robust for use in clinical laboratories and is potentially adaptable to high-throughput assay formats...
  26. pmc Morphogenesis signaling components influence cell cycle regulation by cyclin dependent kinase
    Brian Td Tobe
    Ludwig Center for Metastasis Research, University of Chicago, Chicago, IL 60637, USA
    Cell Div 4:12. 2009
    ..When facing nutrient-limited conditions, Ras2-mediated PKA and MAPK signaling cascades induce a switch from round to filamentous morphology resulting in delayed mitotic progression...
  27. pmc Synthesis enables identification of the cellular target of leucascandrolide A and neopeltolide
    Olesya A Ulanovskaya
    Department of Chemistry, University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA
    Nat Chem Biol 4:418-24. 2008
    ..This result provided the molecular basis for the potent antiproliferative activity of this class of marine macrolides, thus identifying them as new biochemical tools for investigation of eukaryotic energy metabolism...
  28. ncbi request reprint Histone H2AX phosphorylation as a predictor of radiosensitivity and target for radiotherapy
    Neelam Taneja
    Center for Molecular Oncology, Department of Radiation and Cellular Oncology, University of Chicago, Chicago, IL 60637, USA
    J Biol Chem 279:2273-80. 2004
    ..These results suggest that H2AX is a potential molecular target to enhance the effects of radiotherapy...
  29. pmc Poly(ADP-ribose) polymerase inhibitor induces accelerated senescence in irradiated breast cancer cells and tumors
    Elena V Efimova
    Ludwig Center for Metastasis Research, The University of Chicago, Chicago, Illinois 60637, USA
    Cancer Res 70:6277-82. 2010
    ..PARP inhibition markedly enhanced IRIF persistence and increased breast cancer cell senescence both in vitro and in vivo, arguing for targeting IRIF resolution as a novel therapeutic strategy...
  30. doi request reprint Kinase activation in circulating cells: opportunities for biomarkers for diagnosis and therapeutic monitoring
    Laurie L Parker
    University of Chicago, Ludwig Center for Metastasis Research, Knapp R322, 924 E 57th Street, Chicago, IL 6063, USA
    Expert Opin Med Diagn 2:33-46. 2008
    ..Looking forward, the potential value of signaling patterns as complex biomarkers and the resulting need for future development of robust, multiplexed assays of kinase activation is addressed...
  31. ncbi request reprint Bcl-x(L) complements Saccharomyces cerevisiae genes that facilitate the switch from glycolytic to oxidative metabolism
    Matthew G Vander Heiden
    Department of Medicine, The University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 277:44870-6. 2002
    ..Further characterization of the genes identified in this screen may enhance our understanding of Bcl-x(L) function in mammalian cells, and of cell survival pathways in general...
  32. ncbi request reprint Monitoring changes in the subcellular location of proteins in S. cerevisiae
    Diego Rua
    Center for Molecular Oncology, University of Chicago, IL, USA
    Methods Mol Biol 241:299-311. 2004
  33. ncbi request reprint Optimizing thiophosphorylation in the presence of competing phosphorylation with MALDI-TOF-MS detection
    Laurie L Parker
    Department of Biochemistry and Molecular Biology, University of Chicago, CIS 201, 929 E 57 Street, Chicago, IL 60637, USA
    J Proteome Res 4:1863-6. 2005
    ..Under our ideal conditions, titration of Mn(2+) and ATPgammaS in the presence of Mg(2+) allowed relatively rapid, highly specific thiophosphorylation by Abl tyrosine kinase, both as purified enzyme and in complex cell extracts...
  34. ncbi request reprint Design and implementation of algorithms for focus automation in digital imaging time-lapse microscopy
    Allan J LeSage
    Center for Molecular Oncology, The University of Chicago, Chicago, Illinois 60637, USA
    Cytometry 49:159-69. 2002
    ..We have designed and implemented an autofocusing system that tracks subjects under dynamic conditions and maintains focus within a threshold of discriminability...
  35. ncbi request reprint Peptide chips for the quantitative evaluation of protein kinase activity
    Benjamin T Houseman
    Department of Chemistry, Institute for Biophysical Dynamics, University of Chicago, Chicago, IL 60637, USA
    Nat Biotechnol 20:270-4. 2002
    ..Three inhibitors of the enzyme were quantitatively evaluated in an array format on a single, homogeneous substrate...
  36. pmc Enhanced cell polarity in mutants of the budding yeast cyclin-dependent kinase Cdc28p
    S H Ahn
    Center for Molecular Oncology, The University of Chicago, Chicago, Illinois 60637, USA
    Mol Biol Cell 12:3589-600. 2001
    ..These results suggest that Swe1p, via inhibition of Clb2p/Cdc28p, may mediate much of the effect of Cln1p on filamentous morphogenesis...
  37. ncbi request reprint Mutations in the yeast cyclin-dependent kinase Cdc28 reveal a role in the spindle assembly checkpoint
    A A Kitazono
    Center for Molecular Oncology and Dept of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA
    Mol Genet Genomics 269:672-84. 2003
    ....
  38. ncbi request reprint Cell cycle control of yeast filamentous growth
    D Rua
    Center for Molecular Oncology, The University of Chicago, Chicago, IL 60637, USA
    Curr Opin Microbiol 4:720-7. 2001
    ..Here, we discuss how this cell cycle regulation, in concert with control of transcription, ubiquitin-dependent proteolysis and cytoskeletal polarity, may mediate the switch to filamentous growth...
  39. ncbi request reprint Yng2p-dependent NuA4 histone H4 acetylation activity is required for mitotic and meiotic progression
    J S Choy
    Center for Molecular Oncology, Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 276:43653-62. 2001
    ..Treating yng2 mutants with the histone deacetylase inhibitor trichostatin A suppressed the mitotic delay and restored global histone H4 acetylation, arguing that reduced H4 acetylation may underlie the cell cycle delay...
  40. pmc Regulation of G2/M progression by the STE mitogen-activated protein kinase pathway in budding yeast filamentous growth
    S H Ahn
    Center for Molecular Oncology and Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA
    Mol Biol Cell 10:3301-16. 1999
    ..We infer that the MAPK pathway promotes filamentous growth by a novel mechanism that inhibits mitotic cyclin/CDK complexes and thereby modulates cell shape, budding pattern, and cell-cell connections...
  41. ncbi request reprint Protein-acrylamide copolymer hydrogels for array-based detection of tyrosine kinase activity from cell lysates
    Shawn B Brueggemeier
    Department of Chemical and Biological Engineering, University of Wisconsin Madison, 1415 Engineering Drive, Madison, Wisconsin 53706, USA
    Biomacromolecules 6:2765-75. 2005
    ..These findings also establish a basis for screening tyrosine kinase inhibitors and provide a framework on which protein-protein interactions in other complex systems can be studied...
  42. ncbi request reprint Use of protein-acrylamide copolymer hydrogels for measuring protein concentration and activity
    Shawn B Brueggemeier
    Department of Chemical and Biological Engineering, University of Wisconsin Madison, 1415 Engineering Drive, Madison, WI 53706, USA
    Anal Biochem 329:180-9. 2004
    ..Such a surface attachment strategy should be applicable to the proteomics field and addresses denaturation and dehydration problems associated with protein microarray development...
  43. ncbi request reprint Binding of chromatin-modifying activities to phosphorylated histone H2A at DNA damage sites
    Jessica A Downs
    The Wellcome Trust Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, United Kingdom
    Mol Cell 16:979-90. 2004
    ..Thus, phosphorylation of H2A at DNA damage sites creates a mark recognized by different chromatin modifiers. This interaction leads to stepwise chromatin reconfiguration, allowing efficient DNA repair...