Research Topics
Genomes and Genes | Stephen J KronSummaryAffiliation: University of Chicago Country: USA Publications
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Publications
Filamentous growth in budding yeastS J Kron
Center for Molecular Oncology, University of Chicago, Chicago, IL 60637, USA
Trends Microbiol 5:450-4. 1997..The puzzle of why most strains are nonfilamentous is now resolved. Remarkably, a single point mutation with broad consequences separates these domesticated yeast from their wild ancestors...
Yeast G1 DNA damage checkpoint regulation by H2A phosphorylation is independent of chromatin remodelingAli Javaheri
Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA
Proc Natl Acad Sci U S A 103:13771-6. 2006....- Role of Dot1-dependent histone H3 methylation in G1 and S phase DNA damage checkpoint functions of Rad9Robert Wysocki
Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA
Mol Cell Biol 25:8430-43. 2005..These results indicate a key role for chromatin and methylation of histone H3 Lys 79 in yeast DNA damage signaling... - Response of human prostate cancer cells and tumors to combining PARP inhibition with ionizing radiationJuan Camilo Barreto-Andrade
Ludwig Center for Metastasis Research, The University of Chicago, 5841 South Maryland Avenue, MC 9006, Chicago, IL 60637, USA
Mol Cancer Ther 10:1185-93. 2011.... - Non-catalytic function for ATR in the checkpoint responseTroy D McSherry
Center for Molecular Oncology, University of Chicago, Chicago, Illinois, USA
Cell Cycle 6:2019-30. 2007..Thus, the link between the non-catalytic region of the ATR kinase family and the spindle checkpoint pathway is conserved... - Peptide reporters of kinase activity in whole cell lysatesDing Wu
Ludwig Center for Metastasis Research, The University of Chicago, Chicago, IL 60637, USA
Biopolymers 94:475-86. 2010..These strategies can be generalized to improve the reactivity of most peptide substrates used in heterogeneous kinase assays with cell lysates... - A pairwise chemical genetic screen identifies new inhibitors of glucose transportOlesya A Ulanovskaya
Department of Chemistry, The University of Chicago, Chicago, IL 60637, USA
Chem Biol 18:222-30. 2011.... - Dissection of Rad9 BRCT domain function in the mitotic checkpoint response to telomere uncappingCHINONYE C NNAKWE
Department of Pathology, The University of Chicago, Chicago, IL 60637, USA
DNA Repair (Amst) 8:1452-61. 2009.... 
Photocleavable peptide-conjugated magnetic beads for protein kinase assays by MALDI-TOF MSGuangchang Zhou
Ludwig Center for Metastasis Research, The University of Chicago, Chicago, Illinois 60637, USA
Bioconjug Chem 21:1917-24. 2010....- A magnetic bead-based protein kinase assay with dual detection techniquesGuangchang Zhou
Ludwig Center for Metastasis Research, The University of Chicago, IL 60637, USA
Anal Biochem 408:5-11. 2011..Formatted for 96-well plates and with high-throughput potential, this dual detection kinase assay may provide a rapid, reliable, and inexpensive route to the discovery of small-molecule drug leads... - Photocleavable peptide-oligonucleotide conjugates for protein kinase assays by MALDI-TOF MSGuangchang Zhou
Department of Molecular Genetics and Cell Biology, Gordon Center for Integrative Sciences, W522A, The University of Chicago, Chicago, IL 60637, USA
Mol Biosyst 8:2395-404. 2012..This new assay enables analysis of protein kinase activities in a multiplexed format amenable to screening inhibitors against multiple kinases in parallel, an important capability for drug discovery and predictive diagnostics... - A bead-based activity screen for small-molecule inhibitors of signal transduction in chronic myelogenous leukemia cellsJuliesta E Sylvester
Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, USA
Mol Cancer Ther 9:1469-81. 2010..This method for analyzing a focused signaling network benefits from rigorous statistical analysis and short processing times, thereby offering a powerful tool for drug discovery and clinical testing... - A solid-phase Bcr-Abl kinase assay in 96-well hydrogel platesDing Wu
Ludwig Center for Metastasis Research, University of Chicago, Chicago, IL 60637, USA
Anal Biochem 375:18-26. 2008..This assay provides a practical method to measure the activity of a single kinase present in a whole cell lysate with high sensitivity and specificity as a valuable means for efficient small molecule screening... - Rapid validation of Mascot search results via stable isotope labeling, pair picking, and deconvolution of fragmentation patternsSamuel L Volchenboum
Department of Pediatrics, The University of Chicago, Chicago, Illinois 60637, USA
Mol Cell Proteomics 8:2011-22. 2009..DAT file in less than 6 min (32 spectra/s), revealing high confidence peptide identifications without regard to Mascot score, far faster than manual or other independent validation methods... - Photocleavable peptide hydrogel arrays for MALDI-TOF analysis of kinase activityLaurie L Parker
Department of Biochemistry and Molecular Biology, University of Chicago, CIS 201, 929 E 57th Street, Chicago, IL 60637, USA
Analyst 131:1097-104. 2006..This work serves as proof of principle for modular substrate design strategies for mass spectrometry-readable biosensors... - Control of the yeast cell cycle with a photocleavable alpha-factor analogueLaurie L Parker
Department of Biochemistry and Molecular Biology, University of Chicago, 929 E. 57th Street, CIS W201A, Chicago, IL 60637, USA
Angew Chem Int Ed Engl 45:6322-5. 2006 - Phosphoprotein profiling by PA-GeLC-MS/MSKolbrun Kristjansdottir
Department of Molecular Genetics and Cell Biology, and Ludwig Center for Metastasis Research, The University of Chicago, Chicago, Illinois 60637, USA
J Proteome Res 7:2812-24. 2008.... - Relevance and irrelevance of DNA damage response to radiotherapyPhilip P Connell
Department of Radiation and Cellular Oncology, Center for Molecular Oncology, University of Chicago, 5759 S. Maryland Avenue, MC 9006, Chicago, IL 60637, USA
DNA Repair (Amst) 3:1245-51. 2004..Our goal is to frame the issues that contribute to the apparent disconnect between laboratory discoveries and improvements in clinically relevant therapeutics... - Radiation-inducible immunotherapy for cancer: senescent tumor cells as a cancer vaccineYuru Meng
Ludwig Center for Metastasis Research, The University of Chicago, Chicago, Illinois, USA
Mol Ther 20:1046-55. 2012..Applied to human cancers, radiation-inducible immunotherapy may enhance radiotherapy responses to prevent local recurrence and distant metastasis... - Yeast cell death during DNA damage arrest is independent of caspase or reactive oxygen speciesRobert Wysocki
Center for Molecular Oncology, The University of Chicago, 924 E. 57th Street, Rm. R320, IL 60637, USA
J Cell Biol 166:311-6. 2004..We speculate that during a prolonged cell cycle arrest, cdc13-1 cells reach a critical size and die by cell lysis... - Investigating quantitation of phosphorylation using MALDI-TOF mass spectrometryLaurie Parker
Ludwig Center for Metastasis Research, University of Chicago, Knapp R322, 924 E 57th Street, Chicago, IL 60637, USA
J Mass Spectrom 43:518-27. 2008.... - NuA4 subunit Yng2 function in intra-S-phase DNA damage responseJohn S Choy
Department of Molecular Genetics and Cell Biology Center for Molecular Oncology, University of Chicago, Chicago, Illinois 60637, USA
Mol Cell Biol 22:8215-25. 2002..Our results implicate nucleosomal histone acetylation in maintaining genomic integrity during chromosomal replication... - CDK-dependent Hsp70 Phosphorylation controls G1 cyclin abundance and cell-cycle progressionAndrew W Truman
Ludwig Center for Metastasis Research, The University of Chicago, Chicago, IL 60637, USA
Cell 151:1308-18. 2012..These results establish an active role for Hsp70 chaperones as signal transducers mediating growth control of G1 cyclin abundance and activity... - Morphogenesis signaling components influence cell cycle regulation by cyclin dependent kinaseBrian Td Tobe
Ludwig Center for Metastasis Research, University of Chicago, Chicago, IL 60637, USA
Cell Div 4:12. 2009..When facing nutrient-limited conditions, Ras2-mediated PKA and MAPK signaling cascades induce a switch from round to filamentous morphology resulting in delayed mitotic progression... - Poly(ADP-ribose) polymerase inhibitor induces accelerated senescence in irradiated breast cancer cells and tumorsElena V Efimova
Ludwig Center for Metastasis Research, The University of Chicago, Chicago, Illinois 60637, USA
Cancer Res 70:6277-82. 2010..PARP inhibition markedly enhanced IRIF persistence and increased breast cancer cell senescence both in vitro and in vivo, arguing for targeting IRIF resolution as a novel therapeutic strategy... - Histone H2AX phosphorylation as a predictor of radiosensitivity and target for radiotherapyNeelam Taneja
Center for Molecular Oncology, Department of Radiation and Cellular Oncology, University of Chicago, Chicago, IL 60637, USA
J Biol Chem 279:2273-80. 2004..These results suggest that H2AX is a potential molecular target to enhance the effects of radiotherapy... - Synthesis enables identification of the cellular target of leucascandrolide A and neopeltolideOlesya A Ulanovskaya
Department of Chemistry, University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA
Nat Chem Biol 4:418-24. 2008..This result provided the molecular basis for the potent antiproliferative activity of this class of marine macrolides, thus identifying them as new biochemical tools for investigation of eukaryotic energy metabolism... - Assaying Bcr-Abl kinase activity and inhibition in whole cell extracts by phosphorylation of substrates immobilized on agarose beadsDing Wu
Center for Molecular Oncology and Department of Molecular Genetics and Cell Biology, University of Chicago, IL 60637, USA
Anal Biochem 347:67-76. 2005..This simple kinase assay is sufficiently straightforward and robust for use in clinical laboratories and is potentially adaptable to high-throughput assay formats... - Optimizing thiophosphorylation in the presence of competing phosphorylation with MALDI-TOF-MS detectionLaurie L Parker
Department of Biochemistry and Molecular Biology, University of Chicago, CIS 201, 929 E 57 Street, Chicago, IL 60637, USA
J Proteome Res 4:1863-6. 2005..Under our ideal conditions, titration of Mn(2+) and ATPgammaS in the presence of Mg(2+) allowed relatively rapid, highly specific thiophosphorylation by Abl tyrosine kinase, both as purified enzyme and in complex cell extracts... - Design and implementation of algorithms for focus automation in digital imaging time-lapse microscopyAllan J LeSage
Center for Molecular Oncology, The University of Chicago, Chicago, Illinois 60637, USA
Cytometry 49:159-69. 2002..The tolerance of this system of drift and vibration suggests that it is a practical system for time-lapse imaging in many biological applications... - Monitoring changes in the subcellular location of proteins in S. cerevisiaeDiego Rua
Center for Molecular Oncology, University of Chicago, IL, USA
Methods Mol Biol 241:299-311. 2004 - Bcl-x(L) complements Saccharomyces cerevisiae genes that facilitate the switch from glycolytic to oxidative metabolismMatthew G Vander Heiden
Department of Medicine, The University of Chicago, Chicago, Illinois 60637, USA
J Biol Chem 277:44870-6. 2002..Further characterization of the genes identified in this screen may enhance our understanding of Bcl-x(L) function in mammalian cells, and of cell survival pathways in general... - Peptide chips for the quantitative evaluation of protein kinase activityBenjamin T Houseman
Department of Chemistry, Institute for Biophysical Dynamics, University of Chicago, Chicago, IL 60637, USA
Nat Biotechnol 20:270-4. 2002..Three inhibitors of the enzyme were quantitatively evaluated in an array format on a single, homogeneous substrate... - Enhanced cell polarity in mutants of the budding yeast cyclin-dependent kinase Cdc28pS H Ahn
Center for Molecular Oncology, The University of Chicago, Chicago, Illinois 60637, USA
Mol Biol Cell 12:3589-600. 2001..These results suggest that Swe1p, via inhibition of Clb2p/Cdc28p, may mediate much of the effect of Cln1p on filamentous morphogenesis... - Regulation of G2/M progression by the STE mitogen-activated protein kinase pathway in budding yeast filamentous growthS H Ahn
Center for Molecular Oncology and Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA
Mol Biol Cell 10:3301-16. 1999..We infer that the MAPK pathway promotes filamentous growth by a novel mechanism that inhibits mitotic cyclin/CDK complexes and thereby modulates cell shape, budding pattern, and cell-cell connections... - Yng2p-dependent NuA4 histone H4 acetylation activity is required for mitotic and meiotic progressionJ S Choy
Center for Molecular Oncology, Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA
J Biol Chem 276:43653-62. 2001..Treating yng2 mutants with the histone deacetylase inhibitor trichostatin A suppressed the mitotic delay and restored global histone H4 acetylation, arguing that reduced H4 acetylation may underlie the cell cycle delay... - Cell cycle control of yeast filamentous growthD Rua
Center for Molecular Oncology, The University of Chicago, Chicago, IL 60637, USA
Curr Opin Microbiol 4:720-7. 2001..Here, we discuss how this cell cycle regulation, in concert with control of transcription, ubiquitin-dependent proteolysis and cytoskeletal polarity, may mediate the switch to filamentous growth... - Mutations in the yeast cyclin-dependent kinase Cdc28 reveal a role in the spindle assembly checkpointA A Kitazono
Center for Molecular Oncology and Dept of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA
Mol Genet Genomics 269:672-84. 2003.... - Protein-acrylamide copolymer hydrogels for array-based detection of tyrosine kinase activity from cell lysatesShawn B Brueggemeier
Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, Wisconsin 53706, USA
Biomacromolecules 6:2765-75. 2005..These findings also establish a basis for screening tyrosine kinase inhibitors and provide a framework on which protein-protein interactions in other complex systems can be studied... - Use of protein-acrylamide copolymer hydrogels for measuring protein concentration and activityShawn B Brueggemeier
Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706, USA
Anal Biochem 329:180-9. 2004..Such a surface attachment strategy should be applicable to the proteomics field and addresses denaturation and dehydration problems associated with protein microarray development... - Binding of chromatin-modifying activities to phosphorylated histone H2A at DNA damage sitesJessica A Downs
The Wellcome Trust Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, United Kingdom
Mol Cell 16:979-90. 2004..Thus, phosphorylation of H2A at DNA damage sites creates a mark recognized by different chromatin modifiers. This interaction leads to stepwise chromatin reconfiguration, allowing efficient DNA repair...