Aikaterini Kontrogianni-Konstantopoulos

Summary

Affiliation: University of Maryland
Country: USA

Publications

  1. ncbi The hydrophilic domain of small ankyrin-1 interacts with the two N-terminal immunoglobulin domains of titin
    Aikaterini Kontrogianni-Konstantopoulos
    Department of Physiology, University of Maryland School of Medicine, Baltimore 21201, USA
    J Biol Chem 278:3985-91. 2003
  2. pmc Obscurin is a ligand for small ankyrin 1 in skeletal muscle
    Aikaterini Kontrogianni-Konstantopoulos
    Department of Physiology, University of Maryland, School of Medicine, Baltimore 21201, USA
    Mol Biol Cell 14:1138-48. 2003
  3. pmc Muscle giants: molecular scaffolds in sarcomerogenesis
    Aikaterini Kontrogianni-Konstantopoulos
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Physiol Rev 89:1217-67. 2009
  4. ncbi Obscurin regulates the organization of myosin into A bands
    Aikaterini Kontrogianni-Konstantopoulos
    Department of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Am J Physiol Cell Physiol 287:C209-17. 2004
  5. ncbi Obscurin modulates the assembly and organization of sarcomeres and the sarcoplasmic reticulum
    Aikaterini Kontrogianni-Konstantopoulos
    Department of Physiology, University of Maryland Baltimore, School of Medicine, Baltimore, MD 21201, USA
    FASEB J 20:2102-11. 2006
  6. ncbi De novo myofibrillogenesis in C2C12 cells: evidence for the independent assembly of M bands and Z disks
    Aikaterini Kontrogianni-Konstantopoulos
    Dept of Physiology, School of Medicine, Univ of Maryland at Baltimore, 685 W Baltimore St, Baltimore, MD 21201, USA
    Am J Physiol Cell Physiol 290:C626-37. 2006
  7. ncbi Obscurin: a multitasking muscle giant
    Aikaterini Kontrogianni-Konstantopoulos
    Department of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Muscle Res Cell Motil 26:419-26. 2005
  8. pmc The rho-guanine nucleotide exchange factor domain of obscurin regulates assembly of titin at the Z-disk through interactions with Ran binding protein 9
    Amber L Bowman
    Department of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Mol Biol Cell 19:3782-92. 2008
  9. ncbi Mapping the binding site on small ankyrin 1 for obscurin
    Maegen A Borzok
    Department of Biochemistry and Molecular Biology, University of Maryland, School of Medicine, Baltimore 21201, USA
    J Biol Chem 282:32384-96. 2007
  10. pmc Obscurin interacts with a novel isoform of MyBP-C slow at the periphery of the sarcomeric M-band and regulates thick filament assembly
    Maegen A Ackermann
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Mol Biol Cell 20:2963-78. 2009

Research Grants

  1. M-Line Proteins and A-Band Assembly in Skeletal Muscle
    Aikaterini Kontrogianni Konstantopoulos; Fiscal Year: 2010
  2. M-Line Proteins and A-Band Assembly in Skeletal Muscle
    Aikaterini Kontrogianni Konstantopoulos; Fiscal Year: 2007
  3. M-Line Proteins and A-Band Assembly in Skeletal Muscle
    Aikaterini Kontrogianni Konstantopoulos; Fiscal Year: 2009

Collaborators

  • Robert Bloch
  • Mark Russell
  • James R Sellers
  • Andrei B Borisov
  • Alexander D MacKerell
  • Andrew P Ziman
  • Margaret Westfall
  • Federica Del Monte
  • Aikaterini Kontrogianni Konstantopoulos
  • Maegen A Ackermann
  • Nicole A Perry
  • Amber L Bowman
  • Li Yen R Hu
  • Solomon V Yap
  • Ben Busby
  • Elizabeth Vafiadaki
  • Dawn H Catino
  • Demetrios A Arvanitis
  • Marey Shriver
  • John Strong
  • Chris D Willis
  • William R Randall
  • Evangelia G Kranias
  • Maegen A Borzok
  • Despina Sanoudou
  • Sarah B Geisler
  • Maide O Raeker
  • Puja D Patel
  • Yasuharu Takagi
  • Earl Homsher
  • Jane Valenti
  • Eric Balzer
  • Marie G Mameza
  • Bryan Grabias
  • Yinghua Zhang
  • Christopher W Ward
  • Taiji Oashi
  • Jason M Koontz
  • April K Hartford
  • John C Strong
  • Kimberly N Gregory
  • Hugo Gonzalez-Serratos
  • James D Nicholson
  • Bryan A Mitton
  • Sara S Hirsch
  • Guo Chang Fan
  • Fengyun Su
  • Susan E Lyons

Detail Information

Publications29

  1. ncbi The hydrophilic domain of small ankyrin-1 interacts with the two N-terminal immunoglobulin domains of titin
    Aikaterini Kontrogianni-Konstantopoulos
    Department of Physiology, University of Maryland School of Medicine, Baltimore 21201, USA
    J Biol Chem 278:3985-91. 2003
    ..Our results indicate that titin ZIg1/2 can bind sAnk1 in muscle homogenates and suggest a role for these proteins in organizing the SR around the contractile apparatus at the Z-line...
  2. pmc Obscurin is a ligand for small ankyrin 1 in skeletal muscle
    Aikaterini Kontrogianni-Konstantopoulos
    Department of Physiology, University of Maryland, School of Medicine, Baltimore 21201, USA
    Mol Biol Cell 14:1138-48. 2003
    ..Our findings suggest that obscurin binds sAnk1, and are the first to document a specific and direct interaction between proteins of the sarcomere and the SR...
  3. pmc Muscle giants: molecular scaffolds in sarcomerogenesis
    Aikaterini Kontrogianni-Konstantopoulos
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Physiol Rev 89:1217-67. 2009
    ....
  4. ncbi Obscurin regulates the organization of myosin into A bands
    Aikaterini Kontrogianni-Konstantopoulos
    Department of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Am J Physiol Cell Physiol 287:C209-17. 2004
    ..Thus our findings suggest that the COOH-terminal region of obscurin interacts with sarcomeric myosin and may play a critical role in its ability to assemble into A bands in striated muscle...
  5. ncbi Obscurin modulates the assembly and organization of sarcomeres and the sarcoplasmic reticulum
    Aikaterini Kontrogianni-Konstantopoulos
    Department of Physiology, University of Maryland Baltimore, School of Medicine, Baltimore, MD 21201, USA
    FASEB J 20:2102-11. 2006
    ..Our findings suggest that obscurin is required for the assembly of the M-band and A-band and for the regular alignment of the network SR around the contractile apparatus...
  6. ncbi De novo myofibrillogenesis in C2C12 cells: evidence for the independent assembly of M bands and Z disks
    Aikaterini Kontrogianni-Konstantopoulos
    Dept of Physiology, School of Medicine, Univ of Maryland at Baltimore, 685 W Baltimore St, Baltimore, MD 21201, USA
    Am J Physiol Cell Physiol 290:C626-37. 2006
    ....
  7. ncbi Obscurin: a multitasking muscle giant
    Aikaterini Kontrogianni-Konstantopoulos
    Department of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Muscle Res Cell Motil 26:419-26. 2005
    ....
  8. pmc The rho-guanine nucleotide exchange factor domain of obscurin regulates assembly of titin at the Z-disk through interactions with Ran binding protein 9
    Amber L Bowman
    Department of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Mol Biol Cell 19:3782-92. 2008
    ..Our results suggest that the Rho-GEF domain interacts with RanBP9 and that both can interact with the N-terminal region of titin to influence the formation of the Z-disk and A/I junction...
  9. ncbi Mapping the binding site on small ankyrin 1 for obscurin
    Maegen A Borzok
    Department of Biochemistry and Molecular Biology, University of Maryland, School of Medicine, Baltimore 21201, USA
    J Biol Chem 282:32384-96. 2007
    ..Thus, structural and mutational studies of the binding region on sAnk1 for obscurin suggest that it consists of two ankyrin repeats with very similar structures...
  10. pmc Obscurin interacts with a novel isoform of MyBP-C slow at the periphery of the sarcomeric M-band and regulates thick filament assembly
    Maegen A Ackermann
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Mol Biol Cell 20:2963-78. 2009
    ..Collectively, our experiments identify a new ligand of obscurin at the M-band, MyBP-C slow variant-1 and suggest that their interaction contributes to the assembly of M- and A-bands...
  11. pmc Electrostatic interactions mediate binding of obscurin to small ankyrin 1: biochemical and molecular modeling studies
    Ben Busby
    Department of Physiology, University of Maryland School of Medicine, Baltimore, MD, USA
    J Mol Biol 408:321-34. 2011
    ..Our results are consistent with a model in which electrostatic interactions between specific pairs of side chains on obscurin and sAnk1 promote binding and complex formation...
  12. pmc Integrity of the network sarcoplasmic reticulum in skeletal muscle requires small ankyrin 1
    Maegen A Ackermann
    Department of Biochemistry and Molecular Biology, Therapeutics University of Maryland, Baltimore, MD 21201, USA
    J Cell Sci 124:3619-30. 2011
    ..Consistent with the loss of SERCA, uptake and release of Ca(2+) were significantly inhibited. Our results show that sAnk1 stabilizes the nSR and that its absence causes the nSR to fragment into distinct membrane compartments...
  13. pmc Different obscurin isoforms localize to distinct sites at sarcomeres
    Amber L Bowman
    Department of Physiology, University of Maryland School of Medicine, 655 W Baltimore St, MD 21201, USA
    FEBS Lett 581:1549-54. 2007
    ..Additional isoforms, which lack one or more of these epitopes, are present at the Z-disk and Z/I junction...
  14. doi Loss of giant obscurins promotes breast epithelial cell survival through apoptotic resistance
    Nicole A Perry
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA ako
    FASEB J 26:2764-75. 2012
    ..8- and 0.6-fold, respectively). Our findings are the first to pinpoint critical roles for obscurins in cancer development by contributing to the regulation of cell survival...
  15. pmc Loss of actomyosin regulation in distal arthrogryposis myopathy due to mutant myosin binding protein-C slow
    Maegen A Ackermann
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 108 N Greene St, Baltimore, MD 21201, USA
    FASEB J 27:3217-28. 2013
    ....
  16. pmc The kinase domains of obscurin interact with intercellular adhesion proteins
    Li Yen R Hu
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    FASEB J 27:2001-12. 2013
    ..Collectively, our studies demonstrate that the obscurin kinase domains are enzymatically active and may be involved in the regulation of cell adhesion...
  17. doi HAX-1: a multifaceted antiapoptotic protein localizing in the mitochondria and the sarcoplasmic reticulum of striated muscle cells
    Solomon V Yap
    University of Maryland, School of Medicine, Department of Biochemistry and Molecular Biology, Baltimore, MD 21201, USA
    J Mol Cell Cardiol 48:1266-79. 2010
    ..Our findings are the first to present a comprehensive characterization of HAX-1's expression in striated muscles and to provide insights on the mechanisms through which it may modulate apoptosis...
  18. pmc Characterization and comparison of two binding sites on obscurin for small ankyrin 1
    Ben Busby
    Department of Biochemistry and Molecular Biology, University of Maryland, Baltimore, Baltimore, Maryland 21201, United States
    Biochemistry 49:9948-56. 2010
    ..Our results are consistent with a model in which both sequences of obscurin differ significantly in structure but bind to the ankyrin-like repeat motifs of sAnk1 in a similar though not identical manner...
  19. pmc Myosin binding protein-C slow is a novel substrate for protein kinase A (PKA) and C (PKC) in skeletal muscle
    Maegen A Ackermann
    University of Maryland, School of Medicine, Department of Biochemistry and Molecular Biology, 108 North Greene Street, Baltimore, Maryland 21201, United States
    J Proteome Res 10:4547-55. 2011
    ..In brief, our studies are the first to show that the MyBP-C slow subfamily undergoes phosphorylation, which may regulate its activities in normalcy and disease...
  20. doi Obscurins: unassuming giants enter the spotlight
    Nicole A Perry
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    IUBMB Life 65:479-86. 2013
    ..Herein, we present an overview of obscurins with an emphasis on novel findings that demonstrate their heretofore-unsuspected importance in cell signaling and disease progression...
  21. pmc Myosin binding protein-C: a regulator of actomyosin interaction in striated muscle
    Maegen A Ackermann
    Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
    J Biomed Biotechnol 2011:636403. 2011
    ..This review will summarize our current knowledge on MyBP-C and its role in modulating contractility, focusing on its interactions with both myosin and actin filaments in cardiac and skeletal muscles...
  22. doi HAX-1: a family of apoptotic regulators in health and disease
    Solomon V Yap
    Department of Biochemistry and Molecular Biology, University of Maryland, School of Medicine, Baltimore, Maryland 21201, USA
    J Cell Physiol 226:2752-61. 2011
    ....
  23. pmc Myosin binding protein-C slow: an intricate subfamily of proteins
    Maegen A Ackermann
    Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
    J Biomed Biotechnol 2010:652065. 2010
    ....
  24. ncbi Rapid response of cardiac obscurin gene cluster to aortic stenosis: differential activation of Rho-GEF and MLCK and involvement in hypertrophic growth
    Andrei B Borisov
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor 48109, USA
    Biochem Biophys Res Commun 310:910-8. 2003
    ....
  25. ncbi Dynamics of obscurin localization during differentiation and remodeling of cardiac myocytes: obscurin as an integrator of myofibrillar structure
    Andrei B Borisov
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    J Histochem Cytochem 52:1117-27. 2004
    ....
  26. ncbi Essential role of obscurin in cardiac myofibrillogenesis and hypertrophic response: evidence from small interfering RNA-mediated gene silencing
    Andrei B Borisov
    Division of Pediatric Cardiology, Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Histochem Cell Biol 125:227-38. 2006
    ..This demonstrates a vital role for obscurin in myofibrillogenesis and hypertrophic growth...
  27. ncbi Obscurin is required for the lateral alignment of striated myofibrils in zebrafish
    Maide O Raeker
    Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan 48109, USA
    Dev Dyn 235:2018-29. 2006
    ..The diminished capacity to generate and organize new myofibrils in response to obscurin depletion suggests that it may have a vital role in the causation of or adaptation to cardiac and skeletal myopathies...
  28. ncbi Histidine-rich Ca-binding protein interacts with sarcoplasmic reticulum Ca-ATPase
    Demetrios A Arvanitis
    Molecular Biology Division, Center for Basic Research, Foundation for Biomedical Research of the Academy of Athens, Athens, Greece
    Am J Physiol Heart Circ Physiol 293:H1581-9. 2007
    ..Collectively, our data suggest that HRC may play a key role in the regulation of SR Ca cycling through its direct interactions with SERCA2 and triadin, mediating a fine cross talk between SR Ca uptake and release in the heart...
  29. ncbi Phospholamban interacts with HAX-1, a mitochondrial protein with anti-apoptotic function
    Elizabeth Vafiadaki
    Molecular Biology Division, Center for Basic Research, Foundation for Biomedical Research of the Academy of Athens, Soranou Efesiou 4, Athens 115 27, Greece
    J Mol Biol 367:65-79. 2007
    ..These findings suggest a possible link between the Ca(2+) handling by the sarcoplasmic reticulum and cell survival mediated by the PLN/HAX-1 interaction...

Research Grants5

  1. M-Line Proteins and A-Band Assembly in Skeletal Muscle
    Aikaterini Kontrogianni Konstantopoulos; Fiscal Year: 2010
    ..abstract_text> ..
  2. M-Line Proteins and A-Band Assembly in Skeletal Muscle
    Aikaterini Kontrogianni Konstantopoulos; Fiscal Year: 2007
    ..Knowledge gained from the proposed studies will provide new insights into the molecular mechanisms that integrate thick filaments into sarcomeres of normal skeletal fibers and how they are compromised in human muscle disease. ..
  3. M-Line Proteins and A-Band Assembly in Skeletal Muscle
    Aikaterini Kontrogianni Konstantopoulos; Fiscal Year: 2009
    ..abstract_text> ..