Patrick Kochanek

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. pmc Minocycline reduces neuronal death and attenuates microglial response after pediatric asphyxial cardiac arrest
    Minke Tang
    Department of Critical Care Medicine, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Cereb Blood Flow Metab 30:119-29. 2010
  2. doi request reprint Screening of biochemical and molecular mechanisms of secondary injury and repair in the brain after experimental blast-induced traumatic brain injury in rats
    Patrick M Kochanek
    Department of Critical Care Medicine, Safar Center for Resuscitation Research, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    J Neurotrauma 30:920-37. 2013
  3. ncbi request reprint Adenosine A1 receptor knockout mice develop lethal status epilepticus after experimental traumatic brain injury
    Patrick M Kochanek
    Safar Center for Resuscitation Research, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    J Cereb Blood Flow Metab 26:565-75. 2006
  4. doi request reprint Bakken Lecture: the brain, the heart, and therapeutic hypothermia
    Patrick M Kochanek
    Safar Center for Resuscitation Research, Pittsburgh, PA 15260, USA
    Cleve Clin J Med 76:S8-12. 2009
  5. ncbi request reprint Characterization of the effects of adenosine receptor agonists on cerebral blood flow in uninjured and traumatically injured rat brain using continuous arterial spin-labeled magnetic resonance imaging
    Patrick M Kochanek
    Safar Center for Resuscitation Research, The University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA
    J Cereb Blood Flow Metab 25:1596-612. 2005
  6. pmc Therapeutic hypothermia: the Safar vision
    Patrick M Kochanek
    Safar Center for Resuscitation Research, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    J Neurotrauma 26:417-20. 2009
  7. ncbi request reprint Cerebral blood flow at one year after controlled cortical impact in rats: assessment by magnetic resonance imaging
    Patrick M Kochanek
    Department of Critical Care Medicine and Anesthesiology, Center for Resuscitation Research, Pittsburgh, Pennsylvania 15260, USA
    J Neurotrauma 19:1029-37. 2002
  8. pmc Quo vadis 2010? - carpe diem: challenges and opportunities in pediatric traumatic brain injury
    Patrick M Kochanek
    Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Dev Neurosci 32:335-42. 2010
  9. doi request reprint Biomarkers of primary and evolving damage in traumatic and ischemic brain injury: diagnosis, prognosis, probing mechanisms, and therapeutic decision making
    Patrick M Kochanek
    Safar Center for Resuscitation Research, Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    Curr Opin Crit Care 14:135-41. 2008
  10. pmc Therapeutic hypothermia: applications in pediatric cardiac arrest
    Patrick M Kochanek
    Department of Critical Care Medicine, Safar Center for Resuscitation Research, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    J Neurotrauma 26:421-7. 2009

Research Grants

  1. ADENOSINE AND TRAUMATIC BRAIN INJURY
    Patrick Kochanek; Fiscal Year: 2007
  2. PEDIATRIC NEUROINTENSIVE CARE AND RESUSCITATION RESEARCH
    Patrick Kochanek; Fiscal Year: 2007
  3. ADENOSINE AND TRAUMATIC BRAIN INJURY
    Patrick Kochanek; Fiscal Year: 2002

Detail Information

Publications86

  1. pmc Minocycline reduces neuronal death and attenuates microglial response after pediatric asphyxial cardiac arrest
    Minke Tang
    Department of Critical Care Medicine, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Cereb Blood Flow Metab 30:119-29. 2010
    ..The beneficial effect of minocycline suggests an important role for microglia in selective neuronal death after pediatric ACA, and a possible therapeutic target...
  2. doi request reprint Screening of biochemical and molecular mechanisms of secondary injury and repair in the brain after experimental blast-induced traumatic brain injury in rats
    Patrick M Kochanek
    Department of Critical Care Medicine, Safar Center for Resuscitation Research, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    J Neurotrauma 30:920-37. 2013
    ..A robust biochemical/molecular response occurs after blast-induced mTBI, with the body protected from blast and the head constrained to limit motion...
  3. ncbi request reprint Adenosine A1 receptor knockout mice develop lethal status epilepticus after experimental traumatic brain injury
    Patrick M Kochanek
    Safar Center for Resuscitation Research, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    J Cereb Blood Flow Metab 26:565-75. 2006
    ..05) and SE was restricted to the ko mice (83% incidence). Our data suggest a critical endogenous anticonvulsant action of adenosine at A1 receptors early after experimental TBI...
  4. doi request reprint Bakken Lecture: the brain, the heart, and therapeutic hypothermia
    Patrick M Kochanek
    Safar Center for Resuscitation Research, Pittsburgh, PA 15260, USA
    Cleve Clin J Med 76:S8-12. 2009
    ..Future applications of therapeutic hypothermia may include induction of emergency preservation and resuscitation to buy time for damage-control surgery in patients with exsanguination cardiac arrest...
  5. ncbi request reprint Characterization of the effects of adenosine receptor agonists on cerebral blood flow in uninjured and traumatically injured rat brain using continuous arterial spin-labeled magnetic resonance imaging
    Patrick M Kochanek
    Safar Center for Resuscitation Research, The University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA
    J Cereb Blood Flow Metab 25:1596-612. 2005
    ..2-Chloroadenosine produced enduring increases in CBF in uninjured brain and attenuated posttraumatic hypoperfusion. Future studies of adenosine-related therapies in CNS injury should address the role of CBF...
  6. pmc Therapeutic hypothermia: the Safar vision
    Patrick M Kochanek
    Safar Center for Resuscitation Research, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    J Neurotrauma 26:417-20. 2009
    ..A discussion of future potential uses of this therapy in a variety of applications as part of the Safar vision is also presented...
  7. ncbi request reprint Cerebral blood flow at one year after controlled cortical impact in rats: assessment by magnetic resonance imaging
    Patrick M Kochanek
    Department of Critical Care Medicine and Anesthesiology, Center for Resuscitation Research, Pittsburgh, Pennsylvania 15260, USA
    J Neurotrauma 19:1029-37. 2002
    ..This suggests the possibility of remodeling or repackaging of the brain that preserves CBF outside of the cystic lesion...
  8. pmc Quo vadis 2010? - carpe diem: challenges and opportunities in pediatric traumatic brain injury
    Patrick M Kochanek
    Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Dev Neurosci 32:335-42. 2010
    ....
  9. doi request reprint Biomarkers of primary and evolving damage in traumatic and ischemic brain injury: diagnosis, prognosis, probing mechanisms, and therapeutic decision making
    Patrick M Kochanek
    Safar Center for Resuscitation Research, Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    Curr Opin Crit Care 14:135-41. 2008
    ..Novel proteomic, multiplex, and lipidomic methods are also being applied...
  10. pmc Therapeutic hypothermia: applications in pediatric cardiac arrest
    Patrick M Kochanek
    Department of Critical Care Medicine, Safar Center for Resuscitation Research, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    J Neurotrauma 26:421-7. 2009
    ....
  11. ncbi request reprint Pediatric traumatic brain injury: quo vadis?
    Patrick M Kochanek
    Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA
    Dev Neurosci 28:244-55. 2006
    ..Unique therapeutic targets and enhanced therapeutic opportunities, both in the acute phase after injury and in rehabilitation and regeneration, are suggested...
  12. pmc Disruption of Bax protein prevents neuronal cell death but produces cognitive impairment in mice following traumatic brain injury
    Roya Tehranian
    Geriatric Research Educational and Clinical Center, Veterans Administration Pittsburgh Healthcare System, Pittsburgh, Pennsylvania 15206, USA
    J Neurotrauma 25:755-67. 2008
    ..Thus, it is possible that Bax deficiency in the transgenic mice produces developmental behavioral effects, perhaps due to Bax's role in regulating cell death during development...
  13. ncbi request reprint Acute systemic administration of interleukin-10 suppresses the beneficial effects of moderate hypothermia following traumatic brain injury in rats
    Anthony E Kline
    Department of Neurological Surgery and Brain Trauma Research Center, University of Pittsburgh, 3434 Fifth Avenue, Suite 201, Pittsburgh, PA 15260, USA
    Brain Res 937:22-31. 2002
    ..Rather, IL-10 administration suppressed the beneficial effects produced by hypothermia alone after TBI. The mechanism(s) for the negative effects of IL-10 combined with hypothermia after TBI remain to be determined...
  14. ncbi request reprint Survival without brain damage after clinical death of 60-120 mins in dogs using suspended animation by profound hypothermia
    Wilhelm Behringer
    Safar Center for Resuscitation Research, Pittsburgh, PA, USA
    Crit Care Med 31:1523-31. 2003
    ....
  15. ncbi request reprint Administration of adenosine receptor agonists or antagonists after controlled cortical impact in mice: effects on function and histopathology
    Manu R Varma
    Safar Center for Resuscitation Research, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Brain Res 951:191-201. 2002
    ..05 versus other treatment). We suggest that the beneficial effect of the broad spectrum adenosine receptor agonist 2-CA on motor function after CCI is not mediated solely by effects at the A(1) receptor...
  16. pmc Adenosine A1 receptor activation as a brake on the microglial response after experimental traumatic brain injury in mice
    M Lee Haselkorn
    Safar Center for Resuscitation Research, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    J Neurotrauma 27:901-10. 2010
    ..A(1)AR activation is an endogenous inhibitor of the microglial response to TBI, likely via inhibition of proliferation, and this may represent a therapeutic avenue to modulate microglia after TBI...
  17. pmc Resuscitation of traumatic brain injury and hemorrhagic shock with polynitroxylated albumin, hextend, hypertonic saline, and lactated Ringer's: Effects on acute hemodynamics, survival, and neuronal death in mice
    Jennifer L Exo
    Safar Center for Resuscitation Research, Pittsburgh, Pennsylvania, USA
    J Neurotrauma 26:2403-8. 2009
    ..We conclude that the colloids PNA and HEX exhibited more favorable effects on acute resuscitation parameters than HTS or LR, and did not increase hippocampal neuronal death in this model...
  18. ncbi request reprint Innate gender-based proclivity in response to cytotoxicity and programmed cell death pathway
    Lina Du
    Department of Critical Care Medicine, Safar Center for Resuscitation Research, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    J Biol Chem 279:38563-70. 2004
    ....
  19. ncbi request reprint Intranuclear localization of apoptosis-inducing factor (AIF) and large scale DNA fragmentation after traumatic brain injury in rats and in neuronal cultures exposed to peroxynitrite
    Xiaopeng Zhang
    Department of Anesthesiology and Critical Care Medicine, Safar Center for Resuscitation Research, University of Pittsburgh, 3434 Fifth Avenue, Pittsburgh, PA 15260, USA
    J Neurochem 82:181-91. 2002
    ....
  20. ncbi request reprint Emergency preservation and resuscitation with profound hypothermia, oxygen, and glucose allows reliable neurological recovery after 3 h of cardiac arrest from rapid exsanguination in dogs
    Xianren Wu
    Safar Center for Resuscitation Research, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Cereb Blood Flow Metab 28:302-11. 2008
    ....
  21. pmc Mass-spectrometric characterization of phospholipids and their primary peroxidation products in rat cortical neurons during staurosporine-induced apoptosis
    Vladimir A Tyurin
    Center for Free Radical and Antioxidant Health, Pittsburgh, PA, USA
    J Neurochem 107:1614-33. 2008
    ..These oxidation products participate in mitochondrial membrane permeability transition and in PtdSer externalization leading to recognition and uptake of apoptotic cells by professional phagocytes...
  22. pmc Hemorrhagic shock after experimental traumatic brain injury in mice: effect on neuronal death
    Alia Marie Dennis
    Department of Critical Care Medicine, University of Pittsburgh School of Medicine, 3434 Fifth Avenue, Pittsburgh, PA 15260, USA
    J Neurotrauma 26:889-99. 2009
    ..Our data suggest a critical time window for exacerbation of neuronal death by HS after CCI and may have implications for blast injury victims in austere environments where definitive management is delayed...
  23. pmc Genetic disruption of cyclooxygenase-2 does not improve histological or behavioral outcome after traumatic brain injury in mice
    Muzamil Ahmad
    Geriatric Research Educational and Clinical Center, VA Pittsburgh Healthcare System, Pittsburgh, PA 15206, USA
    J Neurosci Res 86:3605-12. 2008
    ..COX-2 gene disruption did not alter Morris water maze performance. Taken together, these results suggest only a minor role for COX-2 activity in determining outcome after TBI in mouse...
  24. ncbi request reprint Suspended animation can allow survival without brain damage after traumatic exsanguination cardiac arrest of 60 minutes in dogs
    Ala Nozari
    Safar Center for Resuscitation Research, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    J Trauma 57:1266-75. 2004
    ..This study is designed to better mimic the clinical scenario of an exsanguinating trauma victim, for whom suspended animation may buy time for resuscitative surgery and delayed resuscitation...
  25. ncbi request reprint Comparison of seven anesthetic agents on outcome after experimental traumatic brain injury in adult, male rats
    Kimberly D Statler
    Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    J Neurotrauma 23:97-108. 2006
    ..Furthermore, the choice of post-injury sedation and analgesia could have important implications on attempts to translate novel therapies from bench to field or bedside...
  26. ncbi request reprint Isoflurane exerts neuroprotective actions at or near the time of severe traumatic brain injury
    Kimberly D Statler
    Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Brain Res 1076:216-24. 2006
    ..e., when discontinued with recovery to tail pinch immediately before injury). Investigators using isoflurane must consider its beneficial effects in the design and interpretation of experimental TBI research...
  27. ncbi request reprint Heme oxygenase 1 in cerebrospinal fluid from infants and children after severe traumatic brain injury
    J mir L Cousar
    Department of Critical Care Medicine, Safar Center for Resuscitation Research, University of Pittsburgh School of Medicine, and Children s Hospital of Pittsburgh, PA 15213, USA
    Dev Neurosci 28:342-7. 2006
    ..HO-1 increases after TBI and appears to be more prominent in infants compared with older children after injury...
  28. ncbi request reprint Neuron-specific enolase and S100B in cerebrospinal fluid after severe traumatic brain injury in infants and children
    Rachel Pardes Berger
    Department of Pediatrics, Pittsburgh Child Advocacy Center, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 5213, USA
    Pediatrics 109:E31. 2002
    ..In adults, CSF and serum concentrations of NSE and S100B have served as markers of neuronal damage after TBI. Neither NSE nor S100B has previously been studied in CSF after TBI in infants or children...
  29. ncbi request reprint Antioxidant Tempol enhances hypothermic cerebral preservation during prolonged cardiac arrest in dogs
    Wilhelm Behringer
    Safar Center for Resuscitation Research, Department of Anesthesiology Critical Care Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Cereb Blood Flow Metab 22:105-17. 2002
    ..The mechanisms involved in this beneficial effect need further clarification...
  30. ncbi request reprint Traumatic brain injury in infants and children: mechanisms of secondary damage and treatment in the intensive care unit
    Hulya Bayir
    Department of Anesthesiology, University of Pittsburgh Medical Center, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    Crit Care Clin 19:529-49. 2003
    ..Meanwhile, practitioners must offer meticulous supportive neurointensive care using clinically proven therapies aimed at minimizing cerebral swelling for the management of pediatric patients who are victims of TBI...
  31. ncbi request reprint Marked gender effect on lipid peroxidation after severe traumatic brain injury in adult patients
    Hulya Bayir
    Safar Center for Resuscitation Research, and Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    J Neurotrauma 21:1-8. 2004
    ..These results established that gender is an important consideration in clinical trial design, particularly in the case of antioxidant strategies...
  32. ncbi request reprint F2-isoprostane and neuron-specific enolase in cerebrospinal fluid after severe traumatic brain injury in infants and children
    Sumeeta Varma
    Safar Center for Resuscitation Research, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    J Neurotrauma 20:781-6. 2003
    ..0426). CSF F2-isoprostane increases early after TBI in infants and children and is correlated with NSE, supporting a role for oxidative stress in the evolution of secondary damage early after severe TBI in infants and children...
  33. ncbi request reprint Intra-mitochondrial poly(ADP-ribosylation) contributes to NAD+ depletion and cell death induced by oxidative stress
    Lina Du
    Department of Critical Care Medicine, University of Pittsburgh, Safar Center for Resuscitation Research, Pittsburgh, Pennsylvania 15260, USA
    J Biol Chem 278:18426-33. 2003
    ..These data implicate intra-mitochondrial poly(ADP-ribosylation) as an important therapeutic target for central nervous system and other diseases associated with oxidative stress and energy failure...
  34. ncbi request reprint Regulation of interstitial excitatory amino acid concentrations after cortical contusion injury
    Marie E Rose
    Department of Neurology, 526 South BST, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Brain Res 935:40-6. 2002
    ..These results suggest that neither increased release of EAAs due to Na+ channel-mediated depolarization nor failure of glutamate reuptake due to glucose deprivation can explain the majority of the increase in EAA(I) following TBI...
  35. doi request reprint Protein nitration and poly-ADP-ribosylation in brain after rapid exsanguination cardiac arrest in a rat model of emergency preservation and resuscitation
    Fei Han
    Safar Center for Resuscitation Research, University of Pittsburgh, Pittsburgh, PA 15260, United States
    Resuscitation 79:301-10. 2008
    ..We hypothesized that 75 min but not 60 min of EPR would be associated with activation of two potential secondary injury cascades in brain as reflected by protein nitration and poly (ADP-ribose) polymerase (PARP) activation...
  36. ncbi request reprint Assessment of antioxidant reserves and oxidative stress in cerebrospinal fluid after severe traumatic brain injury in infants and children
    Hulya Bayir
    Department of Anesthesiology and Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Pediatr Res 51:571-8. 2002
    ..Progressive compromise of antioxidant defenses and evidence of free radical-mediated lipid peroxidation are noted. These markers could be used to monitor antioxidant strategies in clinical trials...
  37. ncbi request reprint A dual role for poly-ADP-ribosylation in spatial memory acquisition after traumatic brain injury in mice involving NAD+ depletion and ribosylation of 14-3-3gamma
    Margaret A Satchell
    Department of Critical Care Medicine, Safar Center for Resuscitation Research, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    J Neurochem 85:697-708. 2003
    ....
  38. doi request reprint Traumatic brain injury in children: recent advances in management
    Michael J Bell
    Department of Critical Care Medicine, Safar Center for Resuscitation Research, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA
    Indian J Pediatr 75:1159-65. 2008
    ..The 2003 guidelines have stimulated important new research. This is reshaping bedside care...
  39. ncbi request reprint Urinary S100B concentrations are increased after brain injury in children: A preliminary study
    Rachel Pardes Berger
    Department of Pediatrics, Child Advocacy Center, Children s Hospital of Pittsburgh, Safar Center for Resuscitation Research, University of Pittsburgh, Pittsburgh, PA, USA
    Pediatr Crit Care Med 7:557-61. 2006
    ..However, increases in serum are rapid and transient and therefore may be of limited use in certain patients. Urinary S100B concentrations may be able to provide information about brain injury in this subgroup of patients...
  40. doi request reprint Assessment of the delta opioid agonist DADLE in a rat model of lethal hemorrhage treated by emergency preservation and resuscitation
    Tomas Drabek
    Safar Center for Resuscitation Research, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, United States
    Resuscitation 77:220-8. 2008
    ..1+/-30.3 in D10 groups, respectively (p=0.3). OPC, NDS and HDS were not significantly different between groups. In conclusion, DADLE failed to confer benefit on functional or histological outcome in our model of prolonged rat EPR...
  41. doi request reprint Effect of inducible nitric oxide synthase on cerebral blood flow after experimental traumatic brain injury in mice
    Lesley M Foley
    Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA
    J Neurotrauma 25:299-310. 2008
    ..Questions remain if this effect represents a homeostatic component of CBF recovery, pathologic vasodilatation linked to inflammation, or NO-mediated facilitation of angiogenesis...
  42. ncbi request reprint Autophagy is increased in mice after traumatic brain injury and is detectable in human brain after trauma and critical illness
    Robert S B Clark
    Department of Critical Care Medicine, The Safar Center for Resuscitation Research and the Brain Trauma Research Center, Pittsburgh, Pennsylvania 15260, USA
    Autophagy 4:88-90. 2008
    ..Taken together, we show that autophagy occurs after both experimental and clinical TBI, and that oxidative stress contributes to overall neuropathology after TBI in mice, at least in part by initiating or influencing autophagy...
  43. ncbi request reprint Autophagy is increased after traumatic brain injury in mice and is partially inhibited by the antioxidant gamma-glutamylcysteinyl ethyl ester
    Yichen Lai
    Department of Critical Care Medicine, The Safar Center for Resuscitation Research and the Brain Trauma Research Center, University of Pittsburgh, Pittsburgh, PA 15260, USA
    J Cereb Blood Flow Metab 28:540-50. 2008
    ..Taken together, these data show that autophagy occurs after experimental TBI, and that oxidative stress contributes to overall neuropathology, in part by initiating or influencing autophagy...
  44. pmc Cytochrome c/cardiolipin relations in mitochondria: a kiss of death
    Valerian E Kagan
    Center for Free Radical and Antioxidant Health, Pittsburgh, PA 15219, USA
    Free Radic Biol Med 46:1439-53. 2009
    ..Finally, new concepts in drug discovery based on the design of mitochondria-targeted inhibitors of cyt c/CL peroxidase and CL peroxidation with antiapoptotic effects are presented...
  45. pmc Effect of short periods of normobaric hyperoxia on local brain tissue oxygenation and cerebrospinal fluid oxidative stress markers in severe traumatic brain injury
    Ava M Puccio
    Department of Neurological Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Neurotrauma 26:1241-9. 2009
    ..Additional studies are required to examine extended periods of normobaric hyperoxia in a larger sample...
  46. pmc Endothelin-1 is increased in cerebrospinal fluid and associated with unfavorable outcomes in children after severe traumatic brain injury
    Rosanne Salonia
    Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh Pennsylvania, USA
    J Neurotrauma 27:1819-25. 2010
    ..This increase in ET-1 may mediate the hypoperfusion or cerebrovascular dysfunction accompanying severe TBI in children. Importantly, hypothermia does not affect the brain's ET-1 response as measured in the CSF...
  47. pmc Adenosine A1 receptor gene variants associated with post-traumatic seizures after severe TBI
    Amy K Wagner
    Department of Physical Medicine and Rehabilitation, University of Pittsburgh, Suite 202, Pittsburgh, PA 15213, USA
    Epilepsy Res 90:259-72. 2010
    ....
  48. pmc How I cool children in neurocritical care
    Ericka L Fink
    Department of Critical Care Medicine, Children s Hospital of Pittsburgh of UPMC, Pittsburgh, PA 15224, USA
    Neurocrit Care 12:414-20. 2010
    ..The objective of this article is to share our hypothermia protocol for cooling children with acute brain injury...
  49. doi request reprint Blood-brain barrier integrity in a rat model of emergency preservation and resuscitation
    Manuella S Lahoud-Rahme
    Safar Center for Resuscitation Research, University of Pittsburgh, Pittsburgh, PA, United States
    Resuscitation 80:484-8. 2009
    ..The objective of this study was to assess BBB permeability in two EPR models in rats, associated with poor outcome. Rats subjected to traumatic brain injury (TBI) and naïve rats served as positive and negative controls, respectively...
  50. doi request reprint Deep hypothermia attenuates microglial proliferation independent of neuronal death after prolonged cardiac arrest in rats
    Tomas Drabek
    Safar Center for Resuscitation Research, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Anesth Analg 109:914-23. 2009
    ..We hypothesized that deep hypothermia and minocycline would attenuate neuronal death and microglial activation and improve outcome after exsanguination CA in rats...
  51. doi request reprint Relationship between increases in pancreatic enzymes and cerebral events in children after traumatic brain injury
    Joan Sanchez De Toledo
    Department of Critical Care Medicine, University of Pittsburgh, Pittsburgh, PA, USA
    Neurocrit Care 11:322-9. 2009
    ....
  52. ncbi request reprint Selective early cardiolipin peroxidation after traumatic brain injury: an oxidative lipidomics analysis
    Hulya Bayir
    Center for Free Radical and Antioxidant Health, University of Pittsburgh, Pittsburgh, PA, USA
    Ann Neurol 62:154-69. 2007
    ..Enhanced lipid peroxidation is well established in traumatic brain injury. However, its molecular targets, identity of peroxidized phospholipid species, and their signaling role have not been deciphered...
  53. ncbi request reprint Exsanguination cardiac arrest in rats treated by 60 min, but not 75 min, emergency preservation and delayed resuscitation is associated with intact outcome
    Tomas Drabek
    Safar Center for Resuscitation Research, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA
    Resuscitation 75:114-23. 2007
    ..This model should serve as a screening model both for testing new pharmacological adjuncts to improve survival after exsanguination CA, and for elucidating the underlying mechanisms of ischemia/reperfusion injury...
  54. ncbi request reprint Enhanced oxidative stress in iNOS-deficient mice after traumatic brain injury: support for a neuroprotective role of iNOS
    Hulya Bayir
    Safar Center for Resuscitation Research, University of Pittsburgh Medical Center, Pennslyvania 15260, USA
    J Cereb Blood Flow Metab 25:673-84. 2005
    ..iNOS also contributes protein nitrosylation and nitration. Colocalization of 3NT with macrophages and MPO suggests generation of nitrating agents by macrophages and/or phagocytosis of nitrated proteins...
  55. ncbi request reprint Mild hypothermia during prolonged cardiopulmonary cerebral resuscitation increases conscious survival in dogs
    Ala Nozari
    Department of Anesthesiology, University of Pittsburgh, Pittsburgh, PA, USA
    Crit Care Med 32:2110-6. 2004
    ..The effect of cooling during CPCR is not known. We hypothesized that mild to moderate hypothermia during CPCR would increase the rate of neurologically intact survival after prolonged cardiac arrest in dogs...
  56. ncbi request reprint Effects of post-injury hypothermia and nerve growth factor infusion on antioxidant enzyme activity in the rat: implications for clinical therapies
    Steven T DeKosky
    Department of Neurology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    J Neurochem 90:998-1004. 2004
    ..These results have implications for clinical treatment of TBI, demonstrating that moderate hypothermia suppresses NGF and the antioxidant response after TBI; the latter cannot be countered by exogenous NGF administration...
  57. ncbi request reprint Identification of inflicted traumatic brain injury in well-appearing infants using serum and cerebrospinal markers: a possible screening tool
    Rachel Pardes Berger
    Department of Pediatrics, Child Advocacy Center, Pittsburgh, Pennsylvania, USA
    Pediatrics 117:325-32. 2006
    ..We investigated whether serum and/or cerebrospinal fluid (CSF) concentrations of neuron-specific enolase (NSE), S100B, and myelin-basic protein (MBP) are sensitive and specific for iTBI in high-risk infants...
  58. ncbi request reprint Time course analysis of hippocampal nerve growth factor and antioxidant enzyme activity following lateral controlled cortical impact brain injury in the rat
    Steven T DeKosky
    Department of Neurology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    J Neurotrauma 21:491-500. 2004
    ..These data demonstrate a complex temporal spectrum of antioxidant enzyme activation following secondary brain injury in the hippocampus, and suggest a selective regulatory role for NGF in this response...
  59. ncbi request reprint boc-Aspartyl(OMe)-fluoromethylketone attenuates mitochondrial release of cytochrome c and delays brain tissue loss after traumatic brain injury in rats
    Robert S B Clark
    Department of Critical Care Medicine, The Safar Center for Resuscitation Research and the Brain Trauma Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Cereb Blood Flow Metab 27:316-26. 2007
    ....
  60. ncbi request reprint Relationships between cerebrospinal fluid markers of excitotoxicity, ischemia, and oxidative damage after severe TBI: the impact of gender, age, and hypothermia
    Amy K Wagner
    Department of Physical Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    J Neurotrauma 21:125-36. 2004
    ..Gender and age differences in TBI pathophysiology should be considered when conducting clinical trials in TBI...
  61. ncbi request reprint Brief induced hypothermia improves outcome after asphyxial cardiopulmonary arrest in juvenile rats
    Ericka L Fink
    Department of Critical Care Medicine, Safar Center for Resuscitation Research, Pittsburgh, PA, USA
    Dev Neurosci 27:191-9. 2005
    ..Similar to adults, infants and children may benefit from induced hypothermia after cardiopulmonary arrest, warranting further study...
  62. ncbi request reprint Systemic hypothermia, but not regional gut hypothermia, improves survival from prolonged hemorrhagic shock in rats
    Xianren Wu
    Safar Center for Resuscitation Research, Pittsburgh, Pennsylvania 15260, USA
    J Trauma 53:654-62. 2002
    ..We hypothesized that cooling the gut alone could improve survival in a rat HS model and avoid potential deleterious effects of systemic hypothermia...
  63. ncbi request reprint Oxidative stress in immature brain after traumatic brain injury
    Hulya Bayir
    Department of Critical Care Medicine, Safar Center for Resuscitation Research, University of Pittsburgh Medical Center, and Department of Environmental and Occupational Health, Chidren s Hospital of Pittsburgh, PA 15213, USA
    Dev Neurosci 28:420-31. 2006
    ..This review focuses on the sources and assessment of oxidative stress and the link between oxidative stress and cell death pathways in the immature brain after experimental and clinical traumatic brain injury...
  64. ncbi request reprint Neuronal NOS-mediated nitration and inactivation of manganese superoxide dismutase in brain after experimental and human brain injury
    Hulya Bayir
    Safar Center for Resuscitation Research, Pittsburgh, Pennsylvania 15260, USA
    J Neurochem 101:168-81. 2007
    ..Nitration and inactivation of MnSOD could lead to self-amplification of oxidative stress in the brain progressively enhancing peroxynitrite production and secondary damage...
  65. ncbi request reprint Mild hypothermia during hemorrhagic shock in rats improves survival without significant effects on inflammatory responses
    Xianren Wu
    Department of Critical Care Medicine, Safar Center for Resuscitation Research, University of Pittsburgh, PA, USA
    Crit Care Med 31:195-202. 2003
    ..To explore the hypothesis that the survival benefit of mild, therapeutic hypothermia during hemorrhagic shock is associated with inhibition of lipid peroxidation and the acute inflammatory response...
  66. doi request reprint Multiplex assessment of serum biomarker concentrations in well-appearing children with inflicted traumatic brain injury
    Rachel P Berger
    Department of Pediatrics, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Pediatr Res 65:97-102. 2009
    ..The results suggest significant changes in the serum biomarker profile after mild ITBI. Future research is needed to determine whether these biomarkers can screen for brain injury in infants with nonspecific symptoms...
  67. ncbi request reprint Effect of hyperventilation on extracellular concentrations of glutamate, lactate, pyruvate, and local cerebral blood flow in patients with severe traumatic brain injury
    Donald W Marion
    Brain Trauma Research Center, Department of Neurological Surgery, University of Pittsburgh School of Medicine, 200 Lothrop Street, Suite B400, Pittsburgh, PA 15213, USA
    Crit Care Med 30:2619-25. 2002
    ..To determine the potential adverse effects of brief periods of hyperventilation commonly used for acute neurologic deterioration...
  68. ncbi request reprint Continuous versus intermittent cerebrospinal fluid drainage after severe traumatic brain injury in children: effect on biochemical markers
    Paul M Shore
    Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Children s Hospital of Pittsburgh, Pittsburgh, PA, USA
    J Neurotrauma 21:1113-22. 2004
    ..The striking difference in biomarker concentration, CSF volume drained, and ICP suggests the need for a randomized trial directly comparing these two approaches in infants and children with severe TBI...
  69. ncbi request reprint Guidelines for the acute medical management of severe traumatic brain injury in infants, children, and adolescents. Chapter 12. Use of hyperventilation in the acute management of severe pediatric traumatic brain injury
    P David Adelson
    University of Pittsburgh Children s Hospital, USA
    Pediatr Crit Care Med 4:S45-8. 2003
  70. ncbi request reprint Serum S100B concentrations are increased after closed head injury in children: a preliminary study
    Rachel Pardes Berger
    Department of Pediatrics, University of Pittsburgh School of Medicine, Graduate School of Public Health, Pittsburgh, Pennsylvania, USA
    J Neurotrauma 19:1405-9. 2002
    ..The increase is transient, lasting less than 12 h after injury, except in children with severe injury. Future research will focus on the possibility of using serum S100B as a screening test for inflicted CHI...
  71. ncbi request reprint Guidelines for the acute medical management of severe traumatic brain injury in infants, children, and adolescents. Chapter 8. Cerebral perfusion pressure
    P David Adelson
    University of Pittsburgh Children s Hospital, USA
    Pediatr Crit Care Med 4:S31-3. 2003
  72. ncbi request reprint Acute etomidate treatment reduces cognitive deficits and histopathology in rats with traumatic brain injury
    C Edward Dixon
    Department of Neurological Surgery and Safar Center for Resuscitation Research, University of Pittsburgh, PA 15260, USA
    Crit Care Med 31:2222-7. 2003
    ..To test the hypothesis that etomidate treatment improves functional, cognitive, and histologic outcome after experimental traumatic brain injury...
  73. ncbi request reprint Transgenic mice that overexpress the anti-apoptotic Bcl-2 protein have improved histological outcome but unchanged behavioral outcome after traumatic brain injury
    Roya Tehranian
    Geriatric Research Educational and Clinical Center, V A Pittsburgh Healthcare Center, and Department of Neurology, University of Pittsburgh, PA 15261, USA
    Brain Res 1101:126-35. 2006
    ..These results suggest that overexpression of Bcl-2 is only partially neuroprotective and other members of this protein family may prove to be more important in protecting neurons from cell death...
  74. ncbi request reprint Cytochrome c, a biomarker of apoptosis, is increased in cerebrospinal fluid from infants with inflicted brain injury from child abuse
    Margaret A Satchell
    Department of Critical Care Medicine, The Safar Center for Resuscitation Research, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    J Cereb Blood Flow Metab 25:919-27. 2005
    ..The degree of apoptosis after TBI also appears to be gender-dependent. Development of strategies targeting apoptosis after TBI, particularly in victims of child abuse and in girls, appears justified...
  75. ncbi request reprint Prolonged deep hypothermic circulatory arrest in rats can be achieved without cognitive deficits
    Tomas Drabek
    Safar Center for Resuscitation Research, University of Pittsburgh, School of Medicine, Pittsburgh PA 15260, USA
    Life Sci 81:543-52. 2007
    ..sham, and no cognitive deficits were seen in either group vs. sham. Future studies with longer DHCA duration will be necessary to provide targets to assess novel preservation strategies...
  76. ncbi request reprint Cerebrospinal fluid biomarkers versus glasgow coma scale and glasgow outcome scale in pediatric traumatic brain injury: the role of young age and inflicted injury
    Paul M Shore
    Department of Pediatrics, University of Texas Southwestern Medical Center, Division of Critical Care Services, Children s Medical Center of Dallas, 1935 Motor Street, Dallas, TX 73235, USA
    J Neurotrauma 24:75-86. 2007
    ..Although further, prospective study is warranted, these findings suggest important limitations in our current ability to assess injury severity in this important population...
  77. ncbi request reprint Emergency preservation and resuscitation improve survival after 15 minutes of normovolemic cardiac arrest in pigs
    Andreas Janata
    Department of Emergency Medicine, Medical University of Vienna, Austria
    Crit Care Med 35:2785-91. 2007
    ....
  78. ncbi request reprint Proteolysis consistent with activation of caspase-7 after severe traumatic brain injury in humans
    Xiaopeng Zhang
    Department of Critical Care Medicine, The Safar Center for Resuscitation Research, University of Pittsburgh, Pennsylvania 15260, USA
    J Neurotrauma 23:1583-90. 2006
    ..Thus, caspase-7 may play a role in the glial and inflammatory responses, and possibly neuronal death, after TBI in humans...
  79. ncbi request reprint Serum biomarkers after traumatic and hypoxemic brain injuries: insight into the biochemical response of the pediatric brain to inflicted brain injury
    Rachel Pardes Berger
    Department of Pediatrics, Child Advocacy Center, Children s Hospital of Pittsburgh, and Safar Center for Resuscitation Research, University of Pittsburgh, PA 15213, USA
    Dev Neurosci 28:327-35. 2006
    ..This may have important implications for the treatment and prognosis of children with iTBI...
  80. pmc Quantification of poly(ADP-ribose)-modified proteins in cerebrospinal fluid from infants and children after traumatic brain injury
    Ericka L Fink
    Department of Critical Care Medicine, Safar Center for Resuscitation Research and Brain Trauma Research Center, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    J Cereb Blood Flow Metab 28:1523-9. 2008
    ..As PAR-modified protein concentration correlated with age and male sex, developmental and sex-dependent roles for PARP after TBI are implicated...
  81. ncbi request reprint Critical time window for intra-arrest cooling with cold saline flush in a dog model of cardiopulmonary resuscitation
    Ala Nozari
    Department of Anesthesia and Critical Care, Massachusetts General Hospital, Harvard Medical School, 55 Fruit St, Boston, MA 02114 2696
    Circulation 113:2690-6. 2006
    ..In this study, we evaluate the time window for the beneficial effect of intra-arrest cooling in the setting of prolonged CPR in a clinically relevant large-animal model...
  82. ncbi request reprint Induction of profound hypothermia for emergency preservation and resuscitation allows intact survival after cardiac arrest resulting from prolonged lethal hemorrhage and trauma in dogs
    Xianren Wu
    Safar Center for Resuscitation Research, University of Pittsburgh, Pittsburgh, PA, USA
    Circulation 113:1974-82. 2006
    ..Previously, we achieved intact survival of dogs from 2 hours of EPR after rapid hemorrhage. We tested the hypothesis that EPR would achieve good outcome if prolonged hemorrhage preceded cardiac arrest...
  83. ncbi request reprint Murine orthostatic response during prolonged vertical studies: effect on cerebral blood flow measured by arterial spin-labeled MRI
    Lesley M Foley
    Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, PA 15260, USA
    Magn Reson Med 54:798-806. 2005
    ..This suggests that using an HU vertical position for MRI in mice may have broader implications, especially for studies that rely on CBF (such as BOLD and fMRI)...
  84. ncbi request reprint Serum neuron-specific enolase, S100B, and myelin basic protein concentrations after inflicted and noninflicted traumatic brain injury in children
    Rachel Pardes Berger
    Department of Pediatrics, Child Advocacy Center, Children s Hospital of Pittsburgh, Pennsylvania 15217, USA
    J Neurosurg 103:61-8. 2005
    ..The authors investigated whether serum concentrations of biomarkers were increased after noninflicted (n)TBI and iTBI in pediatric cases of varying severity...
  85. ncbi request reprint Should we add stem cells to the code cart in resuscitation of heatstroke?
    Xianren Wu
    Crit Care Med 33:1458-9. 2005
  86. ncbi request reprint Regulation of interstitial excitatory amino acid concentrations after cortical contusion injury
    Marie E Rose
    Department of Neurology, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Brain Res 943:15-22. 2002
    ..These results suggest that neither increased release of EAAs due to Na+ channel-mediated depolarization nor failure of glutamate reuptake due to glucose deprivation can explain the majority of the increase in EAA(I) following TBI...

Research Grants12

  1. ADENOSINE AND TRAUMATIC BRAIN INJURY
    Patrick Kochanek; Fiscal Year: 2007
    ..These studies explore the most promising adenosine-based therapy for TBI-A2a receptor antagonists. Our bench to bedside track record ensures translation to the clinic. ..
  2. PEDIATRIC NEUROINTENSIVE CARE AND RESUSCITATION RESEARCH
    Patrick Kochanek; Fiscal Year: 2007
    ..Training is carried out in ~50,000 sq. ft. of fully equipped lab space. Administrative/technical staff is available, as is ample case material for clinical research. Affirmative action recruitment efforts are in place. ..
  3. ADENOSINE AND TRAUMATIC BRAIN INJURY
    Patrick Kochanek; Fiscal Year: 2002
    ..If successful, these studies will set the stage for a clinical trial, and provide important mechanistic information on the role of adenosine after TBI in humans. ..