Paul S Knoepfler

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Endogenous mammalian histone H3.3 exhibits chromatin-related functions during development
    Kelly M Bush
    Department of Cell Biology and Human Anatomy, University of California Davis School of Medicine, 4303 Tupper Hall, Davis, CA, 95616, USA
    Epigenetics Chromatin 6:7. 2013
  2. pmc Call for fellowship programs in stem cell-based regenerative and cellular medicine: new stem cell training is essential for physicians
    Paul S Knoepfler
    Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Tupper Hall 4303, Davis, CA 95616, USA
    Regen Med 8:223-5. 2013
  3. pmc Key anticipated regulatory issues for clinical use of human induced pluripotent stem cells
    Paul S Knoepfler
    Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Davis, Tupper Hall 4303, Davis, CA 95616, USA
    Regen Med 7:713-20. 2012
  4. pmc Deconstructing stem cell tumorigenicity: a roadmap to safe regenerative medicine
    Paul S Knoepfler
    Department of Cell Biology and Human Anatomy and Stem Cell Program, University of California Davis School of Medicine, Sacramento, CA, USA
    Stem Cells 27:1050-6. 2009
  5. doi request reprint Why myc? An unexpected ingredient in the stem cell cocktail
    Paul S Knoepfler
    Institute of Pediatric Regenerative Medicine, University of California Davis School of Medicine, Sacramento, CA 95817, USA
    Cell Stem Cell 2:18-21. 2008
  6. doi request reprint Stem cells on the brain
    Paul S Knoepfler
    Department of Cell Biology and Human Anatomy, Institute of Pediatric Regenerative Medicine, Shriners Hospital for Children Northern California, University of California Davis School of Medicine, Sacramento, CA 95817, USA
    Arch Neurol 65:311-5. 2008
  7. ncbi request reprint Myc goes global: new tricks for an old oncogene
    Paul S Knoepfler
    Department of Cell Biology and Human Anatomy, Institute of Pediatric Regenerative Medicine, Shriners Hospital for Children Northern California, University of California Davis School of Medicine, Davis, California 95616, USA
    Cancer Res 67:5061-3. 2007
  8. pmc Myc and Miz-1 have coordinate genomic functions including targeting Hox genes in human embryonic stem cells
    Natalia Varlakhanova
    Department of Cell Biology and Human Anatomy, University of California Davis School of Medicine, Sacramento, CA, USA
    Epigenetics Chromatin 4:20. 2011
  9. pmc myc maintains embryonic stem cell pluripotency and self-renewal
    Natalia V Varlakhanova
    Department of Cell Biology and Human Anatomy, University of California Davis School of Medicine, Sacramento, CA 95817, USA
    Differentiation 80:9-19. 2010
  10. pmc N-Myc regulates a widespread euchromatic program in the human genome partially independent of its role as a classical transcription factor
    Rebecca Cotterman
    Department of Cell Biology, University of California Davis School of Medicine, Davis, California 95616, USA
    Cancer Res 68:9654-62. 2008

Research Grants

Collaborators

  • Peggy J Farnham
  • Tobias Kind
  • Veronica Martinez-Cerdeno
  • Victor X Jin
  • James Olson
  • Michael Dyer
  • Rodrigo A P Martins
  • Charles J Sherr
  • David H Rowitch
  • Rebecca Cotterman
  • Alice Wey
  • Bonnie L Barrilleaux
  • John W Riggs
  • Natalia V Varlakhanova
  • Robert N Eisenman
  • Kelly M Bush
  • Agnieszka I Laskowski
  • John K Meissen
  • Rebecca F Cotterman
  • Natalia Varlakhanova
  • Tania Habib
  • Ignacio Moreno de Alboran
  • Beryl A Hatton
  • Frederique Zindy
  • Tadashi Okubo
  • Kurt A Swanson
  • Charlotte A Berkes
  • Vanessa Chan
  • Henriette O'Geen
  • Benjamin Tk Yuen
  • Natasha Varlakhanova
  • Benjamin T K Yuen
  • Oliver Fiehn
  • Dinesh K Barupal
  • Keith Bradnam
  • Ian Korf
  • Barbara B Knowles
  • Judy Morgan
  • Wilhelmine N Devries
  • Veronica Martinez Cerdeno
  • David Pleasure
  • Stephen Murray
  • Leah Rae Donahue
  • Jessica M Lemen
  • Sheryl R Krig
  • Leslie Wilson
  • Mark Tsang
  • Heon Park
  • Brian M Iritani
  • David J Rawlings
  • Andrea Nicks
  • Sarah Andrews
  • Suqing Xie
  • Martine F Roussel
  • Anna Marie Kenney
  • Brigid L M Hogan
  • Kai Huang
  • Ishwar Radhakrishnan
  • Richard S Kang
  • Donald A Bergstrom
  • Shaun M Cowley
  • Carol D Laherty
  • Karen J Seaver
  • Bennett H Penn
  • Stephen J Tapscott

Detail Information

Publications29

  1. pmc Endogenous mammalian histone H3.3 exhibits chromatin-related functions during development
    Kelly M Bush
    Department of Cell Biology and Human Anatomy, University of California Davis School of Medicine, 4303 Tupper Hall, Davis, CA, 95616, USA
    Epigenetics Chromatin 6:7. 2013
    ..3 in mammalian cells and during development has been less thoroughly investigated. To address this gap, we report the production and phenotypic analysis of mice and cells with targeted disruption of the H3.3-encoding gene, H3f3b...
  2. pmc Call for fellowship programs in stem cell-based regenerative and cellular medicine: new stem cell training is essential for physicians
    Paul S Knoepfler
    Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Tupper Hall 4303, Davis, CA 95616, USA
    Regen Med 8:223-5. 2013
    ..The USA is used here as an example of a medical sphere in which it can be argued that such training would be helpful, however such programs would be quite helpful globally...
  3. pmc Key anticipated regulatory issues for clinical use of human induced pluripotent stem cells
    Paul S Knoepfler
    Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Davis, Tupper Hall 4303, Davis, CA 95616, USA
    Regen Med 7:713-20. 2012
    ..In this article, I discuss the potential regulatory issues facing hiPSCs and propose some potential changes in the direction of the field in response...
  4. pmc Deconstructing stem cell tumorigenicity: a roadmap to safe regenerative medicine
    Paul S Knoepfler
    Department of Cell Biology and Human Anatomy and Stem Cell Program, University of California Davis School of Medicine, Sacramento, CA, USA
    Stem Cells 27:1050-6. 2009
    ..Finally, the most promising emerging approaches for overcoming the challenges of stem cell tumorigenicity are highlighted...
  5. doi request reprint Why myc? An unexpected ingredient in the stem cell cocktail
    Paul S Knoepfler
    Institute of Pediatric Regenerative Medicine, University of California Davis School of Medicine, Sacramento, CA 95817, USA
    Cell Stem Cell 2:18-21. 2008
    ..Here the possible mechanisms by which myc controls self-renewal and pluripotency are discussed...
  6. doi request reprint Stem cells on the brain
    Paul S Knoepfler
    Department of Cell Biology and Human Anatomy, Institute of Pediatric Regenerative Medicine, Shriners Hospital for Children Northern California, University of California Davis School of Medicine, Sacramento, CA 95817, USA
    Arch Neurol 65:311-5. 2008
  7. ncbi request reprint Myc goes global: new tricks for an old oncogene
    Paul S Knoepfler
    Department of Cell Biology and Human Anatomy, Institute of Pediatric Regenerative Medicine, Shriners Hospital for Children Northern California, University of California Davis School of Medicine, Davis, California 95616, USA
    Cancer Res 67:5061-3. 2007
    ..These findings suggest actions for Myc that extend beyond the traditional concept of a targeted gene regulator...
  8. pmc Myc and Miz-1 have coordinate genomic functions including targeting Hox genes in human embryonic stem cells
    Natalia Varlakhanova
    Department of Cell Biology and Human Anatomy, University of California Davis School of Medicine, Sacramento, CA, USA
    Epigenetics Chromatin 4:20. 2011
    ..abstract:..
  9. pmc myc maintains embryonic stem cell pluripotency and self-renewal
    Natalia V Varlakhanova
    Department of Cell Biology and Human Anatomy, University of California Davis School of Medicine, Sacramento, CA 95817, USA
    Differentiation 80:9-19. 2010
    ....
  10. pmc N-Myc regulates a widespread euchromatic program in the human genome partially independent of its role as a classical transcription factor
    Rebecca Cotterman
    Department of Cell Biology, University of California Davis School of Medicine, Davis, California 95616, USA
    Cancer Res 68:9654-62. 2008
    ..These findings support a new dual model for Myc chromatin function with important implications for the role of Myc in cancer and stem cell biology, including that of induced pluripotent stem cells...
  11. pmc c-myc and N-myc promote active stem cell metabolism and cycling as architects of the developing brain
    Alice Wey
    Institute of Pediatric Regenerative Medicine, Department of Cell Biology and Human Anatomy, University of California Davis School of Medicine, Shriners Hospital for Children Northern California, Sacramento, CA 95817, USA
    Oncotarget 1:120-30. 2010
    ....
  12. pmc Induced pluripotency and oncogenic transformation are related processes
    John W Riggs
    Department of Cell Biology and Human Anatomy, University of California Davis School of Medicine, Sacramento, California 95616, USA
    Stem Cells Dev 22:37-50. 2013
    ..Taken together, these findings support a model in which OF and iPSCs are related, yet distinct cell types, and in which induced pluripotency and induced tumorigenesis are similar processes...
  13. pmc N-Myc and GCN5 regulate significantly overlapping transcriptional programs in neural stem cells
    Veronica Martinez-Cerdeno
    Department of Pathology and Laboratory Medicine, University of California Davis School of Medicine, Sacramento, California, United States of America
    PLoS ONE 7:e39456. 2012
    ..Thus, we have defined an important role for GCN5 in NSC and provided evidence that GCN5 is an important Myc transcriptional cofactor in vivo...
  14. doi request reprint Acting locally and globally: Myc's ever-expanding roles on chromatin
    Natalia V Varlakhanova
    Institute of Pediatric Regenerative Medicine, University of California Davis School of Medicine, Davis, CA 95616, USA
    Cancer Res 69:7487-90. 2009
    ..The ability of Myc to act both locally and globally on chromatin may be responsible for its wide-ranging effects on the biology of stem and tumor cells...
  15. pmc N-Myc regulates expression of pluripotency genes in neuroblastoma including lif, klf2, klf4, and lin28b
    Rebecca Cotterman
    Department of Cell Biology and Human Anatomy, and Stem Cell Program, University of California Davis School of Medicine, Shriners Hospital for Children Northern California, Sacramento, California, USA
    PLoS ONE 4:e5799. 2009
    ..They also suggest mechanisms by which Myc proteins more generally contribute to maintenance of pluripotency and self-renewal of ESC as well as to iPSC formation...
  16. pmc Myc binds the pluripotency factor Utf1 through the basic-helix-loop-helix leucine zipper domain
    Agnieszka I Laskowski
    Department of Cell Biology and Human Anatomy, University of California Davis School of Medicine, Sacramento, CA 95817, USA
    Biochem Biophys Res Commun 435:551-6. 2013
    ..A large region of Utf1 was found to be necessary for direct interaction with N-Myc, while the basic helix-loop-helix leucine zipper domain of N-Myc is necessary for direct interaction with Utf1...
  17. doi request reprint Chromatin immunoprecipitation assays for myc and N-myc
    Bonnie L Barrilleaux
    Department of Cell Biology and Human Anatomy, Institute of Pediatric Regenerative Medicine, University of California Davis School of Medicine, Shriners Hospital for Children Northern California, Sacramento, CA, USA
    Methods Mol Biol 1012:117-33. 2013
    ..Key variables are discussed and include the starting quantity of cells or tissue, lysis and sonication conditions, the quantity and quality of antibody used, and the identification of reliable target genes for ChIP validation. ..
  18. pmc Induced pluripotent stem cells show metabolomic differences to embryonic stem cells in polyunsaturated phosphatidylcholines and primary metabolism
    John K Meissen
    University of California Davis Genome Center, University of California Davis, Davis, California, USA
    PLoS ONE 7:e46770. 2012
    ..The identified metabolomics signatures of iPSCs and ESCs may have important implications for functional regulation of maintenance and induction of pluripotency...
  19. pmc c- and N-myc regulate neural precursor cell fate, cell cycle, and metabolism to direct cerebellar development
    Alice Wey
    Institute of Pediatric Regenerative Medicine, University of California Davis School of Medicine, Sacramento, CA 95817, USA
    Cerebellum 9:537-47. 2010
    ..Together these findings indicate that c- and N-Myc direct cerebellar development by maintaining CGNP and NSC populations through inhibiting differentiation as well as directing rapid cell cycling and active cellular metabolism...
  20. ncbi request reprint Nmyc plays an essential role during lung development as a dosage-sensitive regulator of progenitor cell proliferation and differentiation
    Tadashi Okubo
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Development 132:1363-74. 2005
    ..We propose a model in which Nmyc is essential in the developing lung for maintaining a distal population of undifferentiated, proliferating progenitor cells...
  21. ncbi request reprint Neural precursor cycling at sonic speed: N-Myc pedals, GSK-3 brakes
    Paul S Knoepfler
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
    Cell Cycle 5:47-52. 2006
    ..These findings indicate that therapeutic strategies targeting N-myc and the IGF pathway might be effective against medulloblastoma...
  22. pmc Myc influences global chromatin structure
    Paul S Knoepfler
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 4417, USA
    EMBO J 25:2723-34. 2006
    ..This study provides the first evidence for regulation of global chromatin structure by an oncoprotein and may explain the broad effects of Myc on cell behavior and tumorigenesis...
  23. pmc Myc stimulates B lymphocyte differentiation and amplifies calcium signaling
    Tania Habib
    Department of Comparative Medicine, University of Washington, Seattle, WA 98195, USA
    J Cell Biol 179:717-31. 2007
    ....
  24. pmc N-myc coordinates retinal growth with eye size during mouse development
    Rodrigo A P Martins
    Department of Developmental Neurobiology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Genes Dev 22:179-93. 2008
    ..We propose that N-myc lies upstream of the cell cycle machinery in the developing mouse retina and thus coordinates the growth of both the retina and eye through extrinsic cues...
  25. pmc N-myc is essential during neurogenesis for the rapid expansion of progenitor cell populations and the inhibition of neuronal differentiation
    Paul S Knoepfler
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Genes Dev 16:2699-712. 2002
    ..Its effects on proliferation and differentiation appear due, at least in part, to down-regulation of a specific subset of cyclin-dependent kinase inhibitors...
  26. pmc N-Myc and the cyclin-dependent kinase inhibitors p18Ink4c and p27Kip1 coordinately regulate cerebellar development
    Frederique Zindy
    Department of Genetics and Tumor Cell Biology, Howard Hughes Medical Institute, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Proc Natl Acad Sci U S A 103:11579-83. 2006
    ..These results provide definitive genetic evidence that expression of N-Myc and concomitant down-regulation of Ink4c and Kip1 contribute to the proper development of the cerebellum...
  27. ncbi request reprint N-myc is an essential downstream effector of Shh signaling during both normal and neoplastic cerebellar growth
    Beryl A Hatton
    Clinical Research Division, Fred Hutchinson Cancer Research Center, University of Washington, Seattle, WA 98109, USA
    Cancer Res 66:8655-61. 2006
    ....
  28. ncbi request reprint HBP1 and Mad1 repressors bind the Sin3 corepressor PAH2 domain with opposite helical orientations
    Kurt A Swanson
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208 3500, USA
    Nat Struct Mol Biol 11:738-46. 2004
    ..These results not only provide insights into how multiple, unrelated transcription factors recruit the same coregulator, but also have implications for how sequence similarity searches are conducted...
  29. ncbi request reprint Pbx marks genes for activation by MyoD indicating a role for a homeodomain protein in establishing myogenic potential
    Charlotte A Berkes
    Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Mol Cell 14:465-77. 2004
    ..This demonstrates a specific mechanism of targeting MyoD to loci in inactive chromatin and reveals a critical role of homeodomain proteins in marking specific genes for activation in the muscle lineage...

Research Grants2

  1. Analyzing Myc function in neural cancers and development
    Paul Knoepfler; Fiscal Year: 2007
    ..Together these aims will cooperatively address the research goals of defining how myc genes function in neurogenesis and induce neural cancers. ..