SIMON AB KNIGHT

Summary

Affiliation: University of Pennsylvania
Country: USA

Publications

  1. ncbi request reprint Reduction of 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt (XTT) is dependent on CaFRE10 ferric reductase for Candida albicans grown in unbuffered media
    Simon A B Knight
    University of Pennsylvania School of Medicine, Department of Medicine, Division of Hematology Oncology, 730 BRB II III, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    Microbiology 152:2301-8. 2006
  2. pmc Iron acquisition from transferrin by Candida albicans depends on the reductive pathway
    Simon A B Knight
    University of Pennsylvania, Department of Medicine, Division of Hematology Oncology, 731 BRB II III, 421 Curie Blvd, Philadelphia, PA 19104 6160, USA
    Infect Immun 73:5482-92. 2005
  3. ncbi request reprint Reductive iron uptake by Candida albicans: role of copper, iron and the TUP1 regulator
    Simon A B Knight
    Department of Medicine, Division of Hematology Oncology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Microbiology 148:29-40. 2002
  4. ncbi request reprint Candida albicans lacking the frataxin homologue: a relevant yeast model for studying the role of frataxin
    Renata Santos
    Laboratoire d Ingéniérie des Protéines et Contrôle Métabolique, Département de Biologie des Génomes, Institut Jacques Monod, UMR 7592 CNRS Universités Paris 6 and 7, 2 place Jussieu, F 75251 Paris Cedex 05, France
    Mol Microbiol 54:507-19. 2004
  5. ncbi request reprint Iron use for haeme synthesis is under control of the yeast frataxin homologue (Yfh1)
    Emmanuel Lesuisse
    Laboratoire d Ingéniérie des Protéines et Contrôle Métabolique, Département de Biologie des Génomes, Institut Jacques Monod, Unité Mixte de Recherche 7592 CNRS Universités Paris 6 7, 2 place Jussieu, F 75251 Paris Cedex 05, France
    Hum Mol Genet 12:879-89. 2003
  6. ncbi request reprint Siderophore uptake by Candida albicans: effect of serum treatment and comparison with Saccharomyces cerevisiae
    Emmanuel Lesuisse
    Laboratoire d Ingéniérie des Protéines et Contrôle Métabolique, Institut Jacques Monod, Tour 43, Université Paris 7 Paris 6, 2 place Jussieu, 75251 Paris Cedex 05, France
    Yeast 19:329-40. 2002
  7. pmc Role of YHM1, encoding a mitochondrial carrier protein, in iron distribution of yeast
    Emmanuel Lesuisse
    Laboratoire d Ingéniérie des Protéines et Contrôle Métabolique, Institut Jacques Monod, Tour 43, Université Paris 7 Paris 6, 2 place Jussieu, 75251 Paris Cedex 05, France
    Biochem J 378:599-607. 2004
  8. ncbi request reprint Frataxin and mitochondrial carrier proteins, Mrs3p and Mrs4p, cooperate in providing iron for heme synthesis
    Yan Zhang
    Department of Medicine, Division of Hematology Oncology, University of Pennsylvania, Philadelphia, 19104, USA
    J Biol Chem 280:19794-807. 2005
  9. ncbi request reprint Mrs3p, Mrs4p, and frataxin provide iron for Fe-S cluster synthesis in mitochondria
    Yan Zhang
    Department of Medicine, Division of Hematology Oncology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 281:22493-502. 2006
  10. pmc Genome-wide screen for genes with effects on distinct iron uptake activities in Saccharomyces cerevisiae
    Emmanuel Lesuisse
    Laboratoire d Ingéniérie des Protéines et Contrôle Métabolique, Département de Biologie des Génomes, Institut Jacques Monod, Unité Mixte de Recherche 7592 CNRS Universités Paris 6 and 7, France
    Genetics 169:107-22. 2005

Research Grants

  1. Iron regulation in candida albicans
    Simon Knight; Fiscal Year: 2006

Collaborators

Detail Information

Publications10

  1. ncbi request reprint Reduction of 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt (XTT) is dependent on CaFRE10 ferric reductase for Candida albicans grown in unbuffered media
    Simon A B Knight
    University of Pennsylvania School of Medicine, Department of Medicine, Division of Hematology Oncology, 730 BRB II III, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    Microbiology 152:2301-8. 2006
    ..albicans but not in S. cerevisiae. C. albicans possessed multiple iron- and pH-regulated reductase activities capable of reducing tetrazolium salts, but, when grown in unbuffered medium, CaFRE10 was required for XTT reductase activity...
  2. pmc Iron acquisition from transferrin by Candida albicans depends on the reductive pathway
    Simon A B Knight
    University of Pennsylvania, Department of Medicine, Division of Hematology Oncology, 731 BRB II III, 421 Curie Blvd, Philadelphia, PA 19104 6160, USA
    Infect Immun 73:5482-92. 2005
    ..The requirement of FTR1 for virulence in a systemic infection model and its role in transferrin iron uptake raise the possibility that transferrin is a source of iron during systemic C. albicans infections...
  3. ncbi request reprint Reductive iron uptake by Candida albicans: role of copper, iron and the TUP1 regulator
    Simon A B Knight
    Department of Medicine, Division of Hematology Oncology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Microbiology 148:29-40. 2002
    ..The opposite regulation of two iron permease genes and the role of TUP1 indicate that the process of iron acquisition by C. albicans may be more complex and potentially more adaptable than by S. cerevisiae...
  4. ncbi request reprint Candida albicans lacking the frataxin homologue: a relevant yeast model for studying the role of frataxin
    Renata Santos
    Laboratoire d Ingéniérie des Protéines et Contrôle Métabolique, Département de Biologie des Génomes, Institut Jacques Monod, UMR 7592 CNRS Universités Paris 6 and 7, 2 place Jussieu, F 75251 Paris Cedex 05, France
    Mol Microbiol 54:507-19. 2004
    ..There are thus profound differences between S. cerevisiae and C. albicans in terms of haem synthesis, cytochrome turnover and the role of frataxin in these processes...
  5. ncbi request reprint Iron use for haeme synthesis is under control of the yeast frataxin homologue (Yfh1)
    Emmanuel Lesuisse
    Laboratoire d Ingéniérie des Protéines et Contrôle Métabolique, Département de Biologie des Génomes, Institut Jacques Monod, Unité Mixte de Recherche 7592 CNRS Universités Paris 6 7, 2 place Jussieu, F 75251 Paris Cedex 05, France
    Hum Mol Genet 12:879-89. 2003
    ..Yfh1p and Hem15p were shown to interact in vitro by Biacore studies. Our results suggest that Yfh1 mediates iron use by ferrochelatase...
  6. ncbi request reprint Siderophore uptake by Candida albicans: effect of serum treatment and comparison with Saccharomyces cerevisiae
    Emmanuel Lesuisse
    Laboratoire d Ingéniérie des Protéines et Contrôle Métabolique, Institut Jacques Monod, Tour 43, Université Paris 7 Paris 6, 2 place Jussieu, 75251 Paris Cedex 05, France
    Yeast 19:329-40. 2002
    ..In summary, iron uptake from ferrichrome-type siderophores was induced in filamentous C. albicans, and a potential role of this iron acquisition system in pathogenicity should be considered...
  7. pmc Role of YHM1, encoding a mitochondrial carrier protein, in iron distribution of yeast
    Emmanuel Lesuisse
    Laboratoire d Ingéniérie des Protéines et Contrôle Métabolique, Institut Jacques Monod, Tour 43, Université Paris 7 Paris 6, 2 place Jussieu, 75251 Paris Cedex 05, France
    Biochem J 378:599-607. 2004
    ..The phenotypes of Delta yhm1 mutants indicate a role for this mitochondrial transporter in cellular iron homoeostasis...
  8. ncbi request reprint Frataxin and mitochondrial carrier proteins, Mrs3p and Mrs4p, cooperate in providing iron for heme synthesis
    Yan Zhang
    Department of Medicine, Division of Hematology Oncology, University of Pennsylvania, Philadelphia, 19104, USA
    J Biol Chem 280:19794-807. 2005
    ....
  9. ncbi request reprint Mrs3p, Mrs4p, and frataxin provide iron for Fe-S cluster synthesis in mitochondria
    Yan Zhang
    Department of Medicine, Division of Hematology Oncology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 281:22493-502. 2006
    ..These findings show that Mrs3/4p transports iron into mitochondria, whereas frataxin makes iron already within mitochondria available for Fe-S cluster synthesis...
  10. pmc Genome-wide screen for genes with effects on distinct iron uptake activities in Saccharomyces cerevisiae
    Emmanuel Lesuisse
    Laboratoire d Ingéniérie des Protéines et Contrôle Métabolique, Département de Biologie des Génomes, Institut Jacques Monod, Unité Mixte de Recherche 7592 CNRS Universités Paris 6 and 7, France
    Genetics 169:107-22. 2005
    ..Measurements of different iron-uptake activities in the yeast knockout collection make possible distinctions between genes with general effects on iron metabolism and those with pathway-specific effects...

Research Grants1

  1. Iron regulation in candida albicans
    Simon Knight; Fiscal Year: 2006
    ..Extracts prepared from iron starved and iron replete C. albicans cells will be examined for their ability to bind DNA elements identified in Aim1. ..