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Species | Ronald L KleinSummaryAffiliation: University of Florida Country: USA Publications
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Publications
Dopaminergic cell loss induced by human A30P alpha-synuclein gene transfer to the rat substantia nigraRonald L Klein
Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, FL 32610 0267, USA
Hum Gene Ther 13:605-12. 2002..The lack of behavioral deficits, despite the significant cell loss, may reflect pathogenesis similar to that of PD, where greater than 50% losses occur before motor behavior is affected...- Dose and promoter effects of adeno-associated viral vector for green fluorescent protein expression in the rat brainRonald L Klein
Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, Florida 32610, USA
Exp Neurol 176:66-74. 2002..The increased efficiency obtained with vector elements such as the CBA promoter and the WPRE may enhance the ability to genetically modify larger portions of the brain while requiring smaller doses and volumes... - Long-term neuronal effects and disposition of ectopic preproNGF gene transfer into the rat septumKe Wu
Department of Pharmacology and Therapeutics, University of Florida, Gainesville, FL 32610, USA
Hum Gene Ther 14:1463-72. 2003..These results reveal potential long-term benefits as well as concerns for genetically modifying septal NGF gene expression to preserve neuronal viability and function... - Tau gene transfer, but not alpha-synuclein, induces both progressive dopamine neuron degeneration and rotational behavior in the ratRonald L Klein
Department of Pharmacology and Therapeutics, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA
Neurobiol Dis 20:64-73. 2005..Both tau and alpha-synuclein are important for substantia nigra neurodegeneration models in rats, further indicating their potential as therapeutic targets for human diseases involving loss of dopamine neurons... - Measurements of vector-derived neurotrophic factor and green fluorescent protein levels in the brainRonald L Klein
Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, FL 32610 0267, USA
Methods 28:286-92. 2002..AAV serotype may also be important for the spread of expression, since injecting an AAV-5 vector into the hippocampus led to higher-frequency transfection of dentate gyrus granule neurons, suggesting altered tropism relative to AAV-2... - Memory-related deficits following selective hippocampal expression of Swedish mutation amyloid precursor protein in the ratYan Gong
Department of Pharmaceutics, College of Pharmacy, University of Florida, Gainesville, 32610, USA
Exp Neurol 200:371-7. 2006..In conclusion, prolonged and localized APP695Swe expression in hippocampal neurons is sufficient to produce memory deficits without plaque formation or neuronal loss... - Recombinant adeno-associated virus serotype 2 effectively transduces primary rat brain astrocytes and microgliaYan Gong
Department of Pharmaceutics, College of Pharmacy, University of Florida, Gainesville, FL 32610, USA
Brain Res Brain Res Protoc 14:18-24. 2004..However, astrocytes co-cultured with neurons were not transduced. rAAV2/CBA is an important new method for genetic manipulation of brain cells, though this may be modulated by interactions among cell types... - Particle detection, number estimation, and feature measurement in gene transfer studies: optical fractionator stereology integrated with digital image processing and analysisMichael A King
Veterans Administration Medical Center, Research Services 151, University of Florida, Gainesville, FL 32602, USA
Methods 28:293-9. 2002..Digital image processing can be used to facilitate object detection and identification, and objects that meet criteria for counting can be analyzed for a variety of morphometric and optical properties... - Rapid neurofibrillary tangle formation after localized gene transfer of mutated tauRonald L Klein
Department of Pharmacology and Therapeutics, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130, USA
Am J Pathol 164:347-53. 2004..The ability to produce neurofibrillary pathology in adult rodents makes this a useful method to study tau-related neurodegeneration... - Parkin is protective for substantia nigra dopamine neurons in a tau gene transfer neurodegeneration modelRonald L Klein
Department of Pharmacology, Toxicology and Neuroscience, Louisiana State University Health Sciences Center LSUHSC, 1501 Kings Highway, Shreveport, LA 71130, USA
Neurosci Lett 401:130-5. 2006..Parkin therefore protects dopamine neurons against tau as it does against alpha-synuclein, which further supports parkin as a therapeutic target for diseases involving loss of dopamine neurons... - Efficient neuronal gene transfer with AAV8 leads to neurotoxic levels of tau or green fluorescent proteinsRonald L Klein
Department of Pharmacology, Toxicology and Neuroscience, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA
Mol Ther 13:517-27. 2006..While the toxicity observed with GFP expression warrants great caution, the efficient AAV8 is promising for animal models of neurodegenerative diseases and potentially as well for gene therapy of brain diseases... - AAV8, 9, Rh10, Rh43 vector gene transfer in the rat brain: effects of serotype, promoter and purification methodRonald L Klein
Department of Pharmacology, Toxicology, and Neuroscience, Louisiana State University Health Sciences Center, Shreveport, Louisiana, USA
Mol Ther 16:89-96. 2008..The purification method can therefore impact the transduction pattern as well as the results when comparing serotype strengths... - Adeno-associated viruses containing bFGF or BDNF are neuroprotective against excitotoxicityFrank Schuettauf
Department of Ophthalmology, University of Pennsylvania, Scheie Eye Institute, Philadelphia, Pennsylvania, USA
Curr Eye Res 29:379-86. 2004..We tested the possibility of delivering these growth factors to retinal ganglion cells via an adeno-associated viral (AAV) vector and tested their efficacy in two models of excitotoxicity... - Neuropathological and behavioral consequences of adeno-associated viral vector-mediated continuous intrastriatal neurotrophin delivery in a focal ischemia model in ratsGunnar Andsberg
Section of Restorative Neurology, Wallenberg Neuroscience Center, University Hospital, Lund, Sweden
Neurobiol Dis 9:187-204. 2002..We observe that intrastriatal delivery of NGF and BDNF using a viral vector system can mitigate, albeit only moderately, neuronal death following stroke, which leads to detectable functional sparing... - Gene therapy with brain-derived neurotrophic factor as a protection: retinal ganglion cells in a rat glaucoma modelKeith R G Martin
Wilmer Eye Institute, Johns Hopkins University, Baltimore, Maryland 21287, USA
Invest Ophthalmol Vis Sci 44:4357-65. 2003..Overexpression of the BDNF gene protects RGC as estimated by axon counts in a rat glaucoma model, further supporting the potential feasibility of neurotrophic therapy as a complement to the lowering of IOP in the treatment of glaucoma... - Adeno-associated virus vector expressing nerve growth factor enhances cholinergic axonal sprouting after cortical injury in ratsJulio J Ramirez
Department of Psychology, Neuroscience Program, Davidson College, Davidson, North Carolina 28035 7017, USA
J Neurosci 23:2797-803. 2003..Thus, NGF-expressing rAAV vector enhanced the sprouting response of intact cholinergic neurons after unilateral entorhinal lesions in rats... - Suppression of insult-induced neurogenesis in adult rat brain by brain-derived neurotrophic factorElin Larsson
Section of Restorative Neurology, Wallenberg Neuroscience Center, University Hospital, BMC A 11 SE 221 84, Lund, Sweden
Exp Neurol 177:1-8. 2002..Our data indicate that long-term delivery of a neurotrophic factor, which is considered as a novel neuroprotective strategy for human brain diseases, may attenuate intrinsic neuroregenerative responses... - Gene delivery to the eye using adeno-associated viral vectorsKeith R G Martin
Wilmer Eye Institute, Wilmer 122, Johns Hopkins Hospital, 600 North Wolfe Street, Baltimore, MD 21287, USA
Methods 28:267-75. 2002..Our techniques facilitate the study of the pathogenesis of RGC diseases such as glaucoma and the development of novel new treatments based on gene therapy... - Tau expression levels from various adeno-associated virus vector serotypes produce graded neurodegenerative disease statesRonald L Klein
Department of Pharmacology, Toxicology and Neuroscience, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71130, USA
Eur J Neurosci 27:1615-25. 2008..The disease models stemming from the AAV variants AAV9 or AAV10 may be useful for rapid drug screening, particularly for tau diseases that affect the nigrostriatal system, such as progressive supranuclear palsy...