Harry J Klee

Summary

Affiliation: University of Florida
Country: USA

Publications

  1. pmc Flavour compounds in tomato fruits: identification of loci and potential pathways affecting volatile composition
    Sandrine Mathieu
    Plant Molecular and Cellular Biology Program, University of Florida, Horticultural Sciences, PO Box 110690, Gainesville, FL 32611, USA
    J Exp Bot 60:325-37. 2009
  2. doi request reprint Improving the flavor of fresh fruits: genomics, biochemistry, and biotechnology
    Harry J Klee
    University of Florida, Horticultural Sciences, PO Box 110690, Gainesville, FL 23611 0690, USA
    New Phytol 187:44-56. 2010
  3. doi request reprint Purple tomatoes: longer lasting, less disease, and better for you
    Harry J Klee
    Horticultural Sciences, P O Box 110690, University of Florida, Gainesville, FL 32611 0690, USA
    Curr Biol 23:R520-1. 2013
  4. doi request reprint Genetic challenges of flavor improvement in tomato
    Harry J Klee
    University of Florida, Horticultural Sciences, P O Box 110690, Gainesville, FL 32611 0690, USA
    Trends Genet 29:257-62. 2013
  5. pmc Characterization of the branched-chain amino acid aminotransferase enzyme family in tomato
    Gregory S Maloney
    Horticultural Sciences Department and Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611, USA
    Plant Physiol 153:925-36. 2010
  6. pmc Diverse roles of strigolactone signaling in maize architecture and the uncoupling of a branching-specific subnetwork
    Jiahn Chou Guan
    Horticultural Sciences Department, Plant Molecular and Cellular Biology Program, and Genetics Institute, University of Florida, Gainesville, Florida, USA
    Plant Physiol 160:1303-17. 2012
  7. doi request reprint SlCCD7 controls strigolactone biosynthesis, shoot branching and mycorrhiza-induced apocarotenoid formation in tomato
    Jonathan T Vogel
    Horticultural Sciences Department and the Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611, USA
    Plant J 61:300-11. 2010
  8. pmc Identification of genes in the phenylalanine metabolic pathway by ectopic expression of a MYB transcription factor in tomato fruit
    Valeriano Dal Cin
    University of Florida, Horticultural Sciences, Gainesville, Florida 32611 0690, USA
    Plant Cell 23:2738-53. 2011
  9. ncbi request reprint Characterization of three members of the Arabidopsis carotenoid cleavage dioxygenase family demonstrates the divergent roles of this multifunctional enzyme family
    Michele E Auldridge
    Plant Molecular and Cellular Biology Program, PO Box 110690, University of Florida, Gainesville, FL 32611, USA
    Plant J 45:982-93. 2006
  10. doi request reprint Functional analysis of a tomato salicylic acid methyl transferase and its role in synthesis of the flavor volatile methyl salicylate
    Denise Tieman
    Plant Molecular and Cellular Biology Program, Horticultural Sciences, University of Florida, Gainesville, FL 32611 0690, USA
    Plant J 62:113-23. 2010

Collaborators

Detail Information

Publications36

  1. pmc Flavour compounds in tomato fruits: identification of loci and potential pathways affecting volatile composition
    Sandrine Mathieu
    Plant Molecular and Cellular Biology Program, University of Florida, Horticultural Sciences, PO Box 110690, Gainesville, FL 32611, USA
    J Exp Bot 60:325-37. 2009
    ..One QTL is a novel locus affecting fruit carotenoid content on chromosome 2. Volatile emissions from this and other lines indicate that the linear and cyclic apocarotenoid volatiles are probably derived from separate carotenoid pools...
  2. doi request reprint Improving the flavor of fresh fruits: genomics, biochemistry, and biotechnology
    Harry J Klee
    University of Florida, Horticultural Sciences, PO Box 110690, Gainesville, FL 23611 0690, USA
    New Phytol 187:44-56. 2010
    ..However, our knowledge of the regulation of the critical metabolic pathways is still limited. Nonetheless, the pieces are in place for rapid advances to be made in the manipulation of flavor chemistry in the immediate future...
  3. doi request reprint Purple tomatoes: longer lasting, less disease, and better for you
    Harry J Klee
    Horticultural Sciences, P O Box 110690, University of Florida, Gainesville, FL 32611 0690, USA
    Curr Biol 23:R520-1. 2013
    ..These phenotypes are related to the increased antioxidant capacity of the fruits and shed light on the role of oxidative processes in the later stages of fruit ripening. ..
  4. doi request reprint Genetic challenges of flavor improvement in tomato
    Harry J Klee
    University of Florida, Horticultural Sciences, P O Box 110690, Gainesville, FL 32611 0690, USA
    Trends Genet 29:257-62. 2013
    ..However, the need to deliver improved flavor in the context of high yield and long postharvest shelf life still present major challenges...
  5. pmc Characterization of the branched-chain amino acid aminotransferase enzyme family in tomato
    Gregory S Maloney
    Horticultural Sciences Department and Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611, USA
    Plant Physiol 153:925-36. 2010
    ..Together, these results support a model in which the mitochondrial SlBCAT1 and -2 function in BCAA catabolism while the chloroplastic SlBCAT3 and -4 function in BCAA synthesis...
  6. pmc Diverse roles of strigolactone signaling in maize architecture and the uncoupling of a branching-specific subnetwork
    Jiahn Chou Guan
    Horticultural Sciences Department, Plant Molecular and Cellular Biology Program, and Genetics Institute, University of Florida, Gainesville, Florida, USA
    Plant Physiol 160:1303-17. 2012
    ..Our findings indicate that in maize, uncoupling of the Tb1 subnetwork from SL signaling has profoundly altered the balance between conserved roles of SLs in branching and diverse aspects of plant architecture...
  7. doi request reprint SlCCD7 controls strigolactone biosynthesis, shoot branching and mycorrhiza-induced apocarotenoid formation in tomato
    Jonathan T Vogel
    Horticultural Sciences Department and the Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611, USA
    Plant J 61:300-11. 2010
    ..This work demonstrates the diverse roles of MAX3/CCD7 in strigolactone production, shoot branching, source-sink interactions and production of arbuscular mycorrhiza-induced apocarotenoids...
  8. pmc Identification of genes in the phenylalanine metabolic pathway by ectopic expression of a MYB transcription factor in tomato fruit
    Valeriano Dal Cin
    University of Florida, Horticultural Sciences, Gainesville, Florida 32611 0690, USA
    Plant Cell 23:2738-53. 2011
    ..However, while increased levels of these human nutrition-related phenylpropanoids may be desirable, there were no increases in levels of Phe-derived flavor volatiles...
  9. ncbi request reprint Characterization of three members of the Arabidopsis carotenoid cleavage dioxygenase family demonstrates the divergent roles of this multifunctional enzyme family
    Michele E Auldridge
    Plant Molecular and Cellular Biology Program, PO Box 110690, University of Florida, Gainesville, FL 32611, USA
    Plant J 45:982-93. 2006
    ..Rather, it may have a role in synthesis of apocarotenoid flavor and aroma volatiles, especially in maturing seeds where loss of function leads to significantly higher carotenoid levels...
  10. doi request reprint Functional analysis of a tomato salicylic acid methyl transferase and its role in synthesis of the flavor volatile methyl salicylate
    Denise Tieman
    Plant Molecular and Cellular Biology Program, Horticultural Sciences, University of Florida, Gainesville, FL 32611 0690, USA
    Plant J 62:113-23. 2010
    ..Taken together, the results indicate that SlSAMT is critical for methyl salicylate synthesis and methyl salicylate, in turn, likely has an important role in controlling SA synthesis...
  11. pmc Ethylene-regulated floral volatile synthesis in petunia corollas
    Beverly A Underwood
    Department of Environmental Horticulture, University of Florida, Gainesville, Florida 32611, USA
    Plant Physiol 138:255-66. 2005
    ..These results implicate PhBSMT1 and 2 as genes responsible for synthesis of methylbenzoate in petunia...
  12. doi request reprint A Solanum lycopersicum catechol-O-methyltransferase involved in synthesis of the flavor molecule guaiacol
    Melissa H Mageroy
    Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611 0690, USA
    Plant J 69:1043-51. 2012
    ..These lines also showed a trend toward lower catechol levels. Taken together, we concluded that CTOMT1 is a catechol-O-methyltransferase that produces guaiacol in tomato fruit...
  13. pmc Circadian regulation of the PhCCD1 carotenoid cleavage dioxygenase controls emission of beta-ionone, a fragrance volatile of petunia flowers
    Andrew J Simkin
    Horticultural Sciences, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611 0690, USA
    Plant Physiol 136:3504-14. 2004
    ..beta-Ionone emission by flowers occurred principally during daylight hours, paralleling PhCCD1 expression in corollas. The results indicate that PhCCD1 activity and beta-ionone emission are likely regulated at the level of transcript...
  14. ncbi request reprint Fruit-specific suppression of the ethylene receptor LeETR4 results in early-ripening tomato fruit
    Brian M Kevany
    Plant Molecular and Cellular Biology Program, Horticultural Sciences, University of Florida, Gainesville, FL 32611 0690, USA
    Plant Biotechnol J 6:295-300. 2008
    ..These results demonstrate that ethylene receptors probably act as biological clocks regulating the onset of tomato fruit ripening...
  15. ncbi request reprint Ethylene receptor degradation controls the timing of ripening in tomato fruit
    Brian M Kevany
    Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611 0690, USA
    Plant J 51:458-67. 2007
    ..The results are consistent with a model in which receptor levels modulate timing of the onset of fruit ripening by measuring cumulative ethylene exposure...
  16. ncbi request reprint The tomato carotenoid cleavage dioxygenase 1 genes contribute to the formation of the flavor volatiles beta-ionone, pseudoionone, and geranylacetone
    Andrew J Simkin
    Horticultural Sciences, Plant Molecular and Cellular Biology Program, PO Box 110690, University of Florida, Gainesville, FL 32611 0690, USA
    Plant J 40:882-92. 2004
    ....
  17. doi request reprint Carotenoid content impacts flavor acceptability in tomato (Solanum lycopersicum)
    Jonathan T Vogel
    Horticultural Sciences, University of Florida, Gainesville, Florida 32611, USA
    J Sci Food Agric 90:2233-40. 2010
    ..In order to assess the importance of apocarotenoid volatiles in flavor perception and acceptability, we conducted sensory evaluations of near-isogenic carotenoid biosynthetic mutants and their parent, Ailsa Craig...
  18. pmc Ligand-induced alterations in the phosphorylation state of ethylene receptors in tomato fruit
    Yusuke Kamiyoshihara
    Horticultural Sciences, University of Florida, Gainesville, Florida 32611 0690, USA
    Plant Physiol 160:488-97. 2012
    ..We provide insights into the nature of receptor on and off states...
  19. doi request reprint Identification of Solanum habrochaites loci that quantitatively influence tomato fruit ripening-associated ethylene emissions
    Valeriano Dal Cin
    University of Florida, Horticultural Sciences, PO Box 110690, Gainesville, FL, 32611 0690, USA
    Theor Appl Genet 119:1183-92. 2009
    ..Thus, they may identify novel modes of regulation. These results illustrate the utility of wild relatives and their introgression lines to understand regulation of fruit ripening-related processes...
  20. pmc The central role of PhEIN2 in ethylene responses throughout plant development in petunia
    Kenichi Shibuya
    Department of Environmental Horticulture, University of Florida, Gainesville, Florida 32611, USA
    Plant Physiol 136:2900-12. 2004
    ..These results demonstrate that PhEIN2 mediates ethylene signals in a wide range of physiological processes and also indicate the central role of EIN2 in ethylene signal transduction...
  21. ncbi request reprint Tomato phenylacetaldehyde reductases catalyze the last step in the synthesis of the aroma volatile 2-phenylethanol
    Denise M Tieman
    Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, United States
    Phytochemistry 68:2660-9. 2007
    ..The in vivo alteration of volatile emissions is an important step toward altering aroma volatiles in plants...
  22. pmc Multiple hormones act sequentially to mediate a susceptible tomato pathogen defense response
    Philip J O'donnell
    Department of Horticultural Sciences, University of Florida, Gainesville, Florida 32611, USA
    Plant Physiol 133:1181-9. 2003
    ..This sequential action of jasmonate, ethylene, and SA in disease symptom development is different from the hormone interactions observed in many other plant-pathogen interactions...
  23. pmc Ethylene signal transduction. Moving beyond Arabidopsis
    Harry J Klee
    Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611, USA
    Plant Physiol 135:660-7. 2004
  24. pmc Systemic acquired tolerance to virulent bacterial pathogens in tomato
    Anna Block
    Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611 USA
    Plant Physiol 138:1481-90. 2005
    ..SAT was also induced by infection with Pseudomonas syringae pv tomato. These data show that SAT resembles systemic acquired resistance without inhibition of pathogen growth...
  25. doi request reprint The chemical interactions underlying tomato flavor preferences
    Denise Tieman
    Horticultural Sciences Department, University of Florida, Gainesville, FL 32611 0690, USA
    Curr Biol 22:1035-9. 2012
    ..Aroma volatiles make contributions to perceived sweetness independent of sugar concentration, suggesting a novel way to increase perception of sweetness without adding sugar...
  26. doi request reprint The carotenoid cleavage dioxygenase 1 enzyme has broad substrate specificity, cleaving multiple carotenoids at two different bond positions
    Jonathan T Vogel
    Horticultural Sciences Department and the Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611, USA
    J Biol Chem 283:11364-73. 2008
    ....
  27. ncbi request reprint Susceptible to intolerance--a range of hormonal actions in a susceptible Arabidopsis pathogen response
    Philip J O'donnell
    Department of Horticultural Sciences, University of Florida, Gainesville, FL 32611, USA
    Plant J 33:245-57. 2003
    ..In contrast to ethylene, accumulation of these hormones was not found to be dependent on SA action. These results indicate that the plants response to a virulent pathogen is a composite of multiple signaling pathways...
  28. ncbi request reprint Identification of loci affecting flavour volatile emissions in tomato fruits
    Denise M Tieman
    Plant Molecular and Cellular Biology Program, University of Florida, Horticultural Sciences, PO Box 110690 Gainesville, 32611, USA
    J Exp Bot 57:887-96. 2006
    ..In the longer term, the genes responsible for controlling the levels of these chemicals will be important tools for understanding the complex interactions that ultimately integrate to provide the unique flavour of a tomato...
  29. pmc Role of an esterase in flavor volatile variation within the tomato clade
    Charles Goulet
    Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
    Proc Natl Acad Sci U S A 109:19009-14. 2012
    ..These results illustrate at a molecular level how closely related species exhibit major differences in volatile production by altering a volatile-associated catabolic activity...
  30. doi request reprint A 13-lipoxygenase, TomloxC, is essential for synthesis of C5 flavour volatiles in tomato
    Jiyuan Shen
    Horticultural Sciences, University of Florida, PO Box 110690, Gainesville, FL 32611 0690, USA
    J Exp Bot 65:419-28. 2014
    ....
  31. doi request reprint Genetics and control of tomato fruit ripening and quality attributes
    Harry J Klee
    University of Florida, Horticultural Sciences, Gainesville, Florida 32611, USA
    Annu Rev Genet 45:41-59. 2011
    ..Here, we describe how ethylene and the transcription factors associated with the ripening process fit together into a network controlling ripening...
  32. ncbi request reprint Autophosphorylation activity of the Arabidopsis ethylene receptor multigene family
    Patricia Moussatche
    Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611, USA
    J Biol Chem 279:48734-41. 2004
    ....
  33. pmc Tomato aromatic amino acid decarboxylases participate in synthesis of the flavor volatiles 2-phenylethanol and 2-phenylacetaldehyde
    Denise Tieman
    Department of Horticultural Sciences, University of Florida, P O Box 110690, Gainesville, FL 32611 0690, USA
    Proc Natl Acad Sci U S A 103:8287-92. 2006
    ..Besides establishing a biosynthetic route, these results show that it is possible to change phenylalanine-based flavor and aroma volatiles in plants by manipulating expression of a single gene...
  34. ncbi request reprint Transformation of a muskmelon 'Galia' hybrid parental line (Cucumis melo L. var. reticulatus Ser.) with an antisense ACC oxidase gene
    Hector G Núñez-Palenius
    Horticultural Sciences Department, University of Florida, IFAS, Gainesville, FL 32611, USA
    Plant Cell Rep 25:198-205. 2006
    ..These transgenic muskmelon male parental lines have potential for use in the production of 'Galia' F1 hybrids with improved shelf life...
  35. doi request reprint Better fruits and vegetables through sensory analysis
    Linda M Bartoshuk
    College of Dentistry, University of Florida, Gainesville, FL 32611 0690, USA
    Curr Biol 23:R374-8. 2013
    ..The ability to address these questions in a whole food presents exciting opportunities to understand the basic principles of how we select the foods that we eat...
  36. ncbi request reprint Control of ethylene-mediated processes in tomato at the level of receptors
    Harry J Klee
    University of Florida, Horticultural Sciences, PO Box 110690, 1143 Fifield Hall, Gainesville, FL 32611, USA
    J Exp Bot 53:2057-63. 2002
    ..Consistent with this model, a reduction in the overall level of receptor increases ethylene responsiveness of a tissue while higher expression of receptor decreases ethylene sensitivity. Evidence to support this model will be presented...