Research Topics
Species | Harry J KleeSummaryAffiliation: University of Florida Country: USA Publications
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Publications
Flavour compounds in tomato fruits: identification of loci and potential pathways affecting volatile compositionSandrine Mathieu
Plant Molecular and Cellular Biology Program, University of Florida, Horticultural Sciences, PO Box 110690, Gainesville, FL 32611, USA
J Exp Bot 60:325-37. 2009..One QTL is a novel locus affecting fruit carotenoid content on chromosome 2. Volatile emissions from this and other lines indicate that the linear and cyclic apocarotenoid volatiles are probably derived from separate carotenoid pools...
Improving the flavor of fresh fruits: genomics, biochemistry, and biotechnologyHarry J Klee
University of Florida, Horticultural Sciences, PO Box 110690, Gainesville, FL 23611 0690, USA
New Phytol 187:44-56. 2010..However, our knowledge of the regulation of the critical metabolic pathways is still limited. Nonetheless, the pieces are in place for rapid advances to be made in the manipulation of flavor chemistry in the immediate future...- Genetic challenges of flavor improvement in tomatoHarry J Klee
University of Florida, Horticultural Sciences, P O Box 110690, Gainesville, FL 32611 0690, USA Electronic address
Trends Genet 29:257-62. 2013..However, the need to deliver improved flavor in the context of high yield and long postharvest shelf life still present major challenges... - Characterization of the branched-chain amino acid aminotransferase enzyme family in tomatoGregory S Maloney
Horticultural Sciences Department and Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611, USA
Plant Physiol 153:925-36. 2010..Together, these results support a model in which the mitochondrial SlBCAT1 and -2 function in BCAA catabolism while the chloroplastic SlBCAT3 and -4 function in BCAA synthesis... - Identification of genes in the phenylalanine metabolic pathway by ectopic expression of a MYB transcription factor in tomato fruitValeriano Dal Cin
University of Florida, Horticultural Sciences, Gainesville, Florida 32611 0690, USA
Plant Cell 23:2738-53. 2011..However, while increased levels of these human nutrition-related phenylpropanoids may be desirable, there were no increases in levels of Phe-derived flavor volatiles... - A Solanum lycopersicum catechol-O-methyltransferase involved in synthesis of the flavor molecule guaiacolMelissa H Mageroy
Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611 0690, USA
Plant J 69:1043-51. 2012..These lines also showed a trend toward lower catechol levels. Taken together, we concluded that CTOMT1 is a catechol-O-methyltransferase that produces guaiacol in tomato fruit... 
Fruit-specific suppression of the ethylene receptor LeETR4 results in early-ripening tomato fruitBrian M Kevany
Plant Molecular and Cellular Biology Program, Horticultural Sciences, University of Florida, Gainesville, FL 32611 0690, USA
Plant Biotechnol J 6:295-300. 2008..These results demonstrate that ethylene receptors probably act as biological clocks regulating the onset of tomato fruit ripening...- Functional analysis of a tomato salicylic acid methyl transferase and its role in synthesis of the flavor volatile methyl salicylateDenise Tieman
Plant Molecular and Cellular Biology Program, Horticultural Sciences, University of Florida, Gainesville, FL 32611 0690, USA
Plant J 62:113-23. 2010..Taken together, the results indicate that SlSAMT is critical for methyl salicylate synthesis and methyl salicylate, in turn, likely has an important role in controlling SA synthesis... - The chemical interactions underlying tomato flavor preferencesDenise Tieman
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611 0690, USA
Curr Biol 22:1035-9. 2012..Aroma volatiles make contributions to perceived sweetness independent of sugar concentration, suggesting a novel way to increase perception of sweetness without adding sugar... - SlCCD7 controls strigolactone biosynthesis, shoot branching and mycorrhiza-induced apocarotenoid formation in tomatoJonathan T Vogel
Horticultural Sciences Department and the Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611, USA
Plant J 61:300-11. 2010..This work demonstrates the diverse roles of MAX3/CCD7 in strigolactone production, shoot branching, source-sink interactions and production of arbuscular mycorrhiza-induced apocarotenoids... - The carotenoid cleavage dioxygenase 1 enzyme has broad substrate specificity, cleaving multiple carotenoids at two different bond positionsJonathan T Vogel
Horticultural Sciences Department and the Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611, USA
J Biol Chem 283:11364-73. 2008.... - Ligand-induced alterations in the phosphorylation state of ethylene receptors in tomato fruitYusuke Kamiyoshihara
Horticultural Sciences, University of Florida, Gainesville, Florida 32611 0690, USA
Plant Physiol 160:488-97. 2012..We provide insights into the nature of receptor on and off states... - Identification of Solanum habrochaites loci that quantitatively influence tomato fruit ripening-associated ethylene emissionsValeriano Dal Cin
University of Florida, Horticultural Sciences, PO Box 110690, Gainesville, FL, 32611 0690, USA
Theor Appl Genet 119:1183-92. 2009..Thus, they may identify novel modes of regulation. These results illustrate the utility of wild relatives and their introgression lines to understand regulation of fruit ripening-related processes... - Role of an esterase in flavor volatile variation within the tomato cladeCharles Goulet
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
Proc Natl Acad Sci U S A 109:19009-14. 2012..These results illustrate at a molecular level how closely related species exhibit major differences in volatile production by altering a volatile-associated catabolic activity... - Carotenoid content impacts flavor acceptability in tomato (Solanum lycopersicum)Jonathan T Vogel
Horticultural Sciences, University of Florida, Gainesville, Florida 32611, USA
J Sci Food Agric 90:2233-40. 2010..In order to assess the importance of apocarotenoid volatiles in flavor perception and acceptability, we conducted sensory evaluations of near-isogenic carotenoid biosynthetic mutants and their parent, Ailsa Craig... - Ethylene receptor degradation controls the timing of ripening in tomato fruitBrian M Kevany
Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611 0690, USA
Plant J 51:458-67. 2007..The results are consistent with a model in which receptor levels modulate timing of the onset of fruit ripening by measuring cumulative ethylene exposure... - The central role of PhEIN2 in ethylene responses throughout plant development in petuniaKenichi Shibuya
Department of Environmental Horticulture, University of Florida, Gainesville, Florida 32611, USA
Plant Physiol 136:2900-12. 2004..These results demonstrate that PhEIN2 mediates ethylene signals in a wide range of physiological processes and also indicate the central role of EIN2 in ethylene signal transduction... - Susceptible to intolerance--a range of hormonal actions in a susceptible Arabidopsis pathogen responseEric A Schmelz
Department of Horticultural Sciences, University of Florida, Gainesville, FL 32611, USA
Plant J 33:245-57. 2003..In contrast to ethylene, accumulation of these hormones was not found to be dependent on SA action. These results indicate that the plants response to a virulent pathogen is a composite of multiple signaling pathways... - Tomato phenylacetaldehyde reductases catalyze the last step in the synthesis of the aroma volatile 2-phenylethanolDenise M Tieman
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, United States
Phytochemistry 68:2660-9. 2007..The in vivo alteration of volatile emissions is an important step toward altering aroma volatiles in plants... - Genetics and control of tomato fruit ripening and quality attributesHarry J Klee
University of Florida, Horticultural Sciences, Gainesville, Florida 32611, USA
Annu Rev Genet 45:41-59. 2011..Here, we describe how ethylene and the transcription factors associated with the ripening process fit together into a network controlling ripening... - Ethylene signal transduction. Moving beyond ArabidopsisHarry J Klee
Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611, USA
Plant Physiol 135:660-7. 2004 - Autophosphorylation activity of the Arabidopsis ethylene receptor multigene familyPatricia Moussatche
Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611, USA
J Biol Chem 279:48734-41. 2004.... - Control of ethylene-mediated processes in tomato at the level of receptorsHarry J Klee
University of Florida, Horticultural Sciences, PO Box 110690, 1143 Fifield Hall, Gainesville, FL 32611, USA
J Exp Bot 53:2057-63. 2002..Consistent with this model, a reduction in the overall level of receptor increases ethylene responsiveness of a tissue while higher expression of receptor decreases ethylene sensitivity. Evidence to support this model will be presented... - Identification of loci affecting flavour volatile emissions in tomato fruitsDenise M Tieman
Plant Molecular and Cellular Biology Program, University of Florida, Horticultural Sciences, PO Box 110690 Gainesville, 32611, USA
J Exp Bot 57:887-96. 2006..In the longer term, the genes responsible for controlling the levels of these chemicals will be important tools for understanding the complex interactions that ultimately integrate to provide the unique flavour of a tomato... - Diverse roles of strigolactone signaling in maize architecture and the uncoupling of a branching-specific subnetworkJiahn Chou Guan
Horticultural Sciences Department, Plant Molecular and Cellular Biology Program, and Genetics Institute, University of Florida, Gainesville, Florida, USA
Plant Physiol 160:1303-17. 2012..Our findings indicate that in maize, uncoupling of the Tb1 subnetwork from SL signaling has profoundly altered the balance between conserved roles of SLs in branching and diverse aspects of plant architecture... - Tomato aromatic amino acid decarboxylases participate in synthesis of the flavor volatiles 2-phenylethanol and 2-phenylacetaldehydeDenise Tieman
Department of Horticultural Sciences, University of Florida, P O Box 110690, Gainesville, FL 32611 0690, USA
Proc Natl Acad Sci U S A 103:8287-92. 2006..Besides establishing a biosynthetic route, these results show that it is possible to change phenylalanine-based flavor and aroma volatiles in plants by manipulating expression of a single gene... - The tomato carotenoid cleavage dioxygenase 1 genes contribute to the formation of the flavor volatiles beta-ionone, pseudoionone, and geranylacetoneAndrew J Simkin
Horticultural Sciences, Plant Molecular and Cellular Biology Program, PO Box 110690, University of Florida, Gainesville, FL 32611 0690, USA
Plant J 40:882-92. 2004.... - Multiple hormones act sequentially to mediate a susceptible tomato pathogen defense responseEric Schmelz
Department of Horticultural Sciences, University of Florida, Gainesville, Florida 32611, USA
Plant Physiol 133:1181-9. 2003..This sequential action of jasmonate, ethylene, and SA in disease symptom development is different from the hormone interactions observed in many other plant-pathogen interactions... - Characterization of three members of the Arabidopsis carotenoid cleavage dioxygenase family demonstrates the divergent roles of this multifunctional enzyme familyMichele E Auldridge
Plant Molecular and Cellular Biology Program, PO Box 110690, University of Florida, Gainesville, FL 32611, USA
Plant J 45:982-93. 2006..Rather, it may have a role in synthesis of apocarotenoid flavor and aroma volatiles, especially in maturing seeds where loss of function leads to significantly higher carotenoid levels... - Ethylene-regulated floral volatile synthesis in petunia corollasBeverly A Underwood
Department of Environmental Horticulture, University of Florida, Gainesville, Florida 32611, USA
Plant Physiol 138:255-66. 2005..These results implicate PhBSMT1 and 2 as genes responsible for synthesis of methylbenzoate in petunia... - Circadian regulation of the PhCCD1 carotenoid cleavage dioxygenase controls emission of beta-ionone, a fragrance volatile of petunia flowersAndrew J Simkin
Horticultural Sciences, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611-0690, USA
Plant Physiol 136:3504-14. 2004..beta-Ionone emission by flowers occurred principally during daylight hours, paralleling PhCCD1 expression in corollas. The results indicate that PhCCD1 activity and beta-ionone emission are likely regulated at the level of transcript... - Systemic acquired tolerance to virulent bacterial pathogens in tomatoAnna Block
Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611. USA
Plant Physiol 138:1481-90. 2005..SAT was also induced by infection with Pseudomonas syringae pv tomato. These data show that SAT resembles systemic acquired resistance without inhibition of pathogen growth...