Research Topics
Species | Kevin KirkSummaryAffiliation: University of Alabama at Birmingham Country: USA Publications
Research Grants
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Publications
Mechanisms of CFTR regulation by syntaxin 1A and PKASteven Y Chang
Department of Medicine, Section of Pulmonary and Critical Care Medicine, The University of Chicago Hospitals, 5841 S. Maryland Avenue, MC 6026, Chicago, IL 60637, USA
J Cell Sci 115:783-91. 2002..We also show that PKA activation can enhance membrane trafficking in some epithelial cell types, and this is independent from CFTR activation or syntaxin 1A association...
A unified view of cystic fibrosis transmembrane conductance regulator (CFTR) gating: combining the allosterism of a ligand-gated channel with the enzymatic activity of an ATP-binding cassette (ABC) transporterKevin L Kirk
Department of Physiology and Biophysics and the Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
J Biol Chem 286:12813-9. 2011..g. unliganded openings and constitutive mutations). Here, we provide a unified view of CFTR gating that combines the allosterism of a ligand-gated channel with its unique enzymatic activity...- ATP-independent CFTR channel gating and allosteric modulation by phosphorylationWei Wang
Department of Physiology and Biophysics, University of Alabama, Birmingham, AL 35294, USA
Proc Natl Acad Sci U S A 107:3888-93. 2010..Our results confirm that (i) CFTR gating exhibits features of protein allostery that are shared with conventional ligand-gated channels and (ii) the R domain modulates CFTR activity independent of ATP-induced NBD dimerization... - Curcumin opens cystic fibrosis transmembrane conductance regulator channels by a novel mechanism that requires neither ATP binding nor dimerization of the nucleotide-binding domainsWei Wang
Department of Physiology and Biophysics and the Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
J Biol Chem 282:4533-44. 2007..The phosphorylation dependence of curcumin activation indicates that the R domain can modulate channel opening without affecting ATP binding to the NBDs or their heterodimerization... - How to review a paperDale J Benos
Department of Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
Adv Physiol Educ 27:47-52. 2003..Proper reviewer conduct is essential for making the peer review process valuable and the journal trustworthy... - Rescuing cystic fibrosis transmembrane conductance regulator (CFTR)-processing mutants by transcomplementationEstelle Cormet-Boyaka
Department of Physiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
Proc Natl Acad Sci U S A 101:8221-6. 2004..These findings indicate that it may be possible to develop CF therapies (e.g., mini-cDNA constructs for gene therapy) that are tailored to specific disease-causing mutants of CFTR... - Cystic fibrosis transmembrane conductance regulator trafficking is mediated by the COPI coat in epithelial cellsJessica Rennolds
Division of Pulmonary, Critical Care, and Sleep Medicine, The Ohio State University, Columbus, Ohio 43210, USA
J Biol Chem 283:833-9. 2008..We discovered that the alpha-, beta-, and gamma-subunits of COPI co-immunoprecipitated with CFTR. Our results indicate that the COPI complex plays a critical role in CFTR trafficking to the PM... - CFTR surface expression and chloride currents are decreased by inhibitors of N-WASP and actin polymerizationRadhika Ganeshan
Department of Physiology and Biophysics, Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, 1918 University Blvd, MCLM 985, Birmingham, AL 35294, USA
Biochim Biophys Acta 1773:192-200. 2007..Our studies implicate a role for N-WASP-mediated actin polymerization in regulating CFTR surface expression and channel activity... - Mutations in the amino terminus of the cystic fibrosis transmembrane conductance regulator enhance endocytosisAsta Jurkuvenaite
Department of Cell Biology, University of Alabama at Birmingham, AL 35294, USA
J Biol Chem 281:3329-34. 2006..These two additive effects contribute to the loss of surface expression and the associated defect in chloride conductance that is consistent with a disease phenotype... - Activating cystic fibrosis transmembrane conductance regulator channels with pore blocker analogsWei Wang
Department of Physiology and Biophysics, Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama 35294-0005, USA
J Biol Chem 280:23622-30. 2005.... - Reversible silencing of CFTR chloride channels by glutathionylationWei Wang
Department of Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, AL 35294, USA
J Gen Physiol 125:127-41. 2005..Our results demonstrate that human CFTR channels are reversibly inhibited by reactive glutathione species, and support an important role of the region proximal to the NBD2 signature sequence in ATP-dependent channel opening... - A macromolecular complex of beta 2 adrenergic receptor, CFTR, and ezrin/radixin/moesin-binding phosphoprotein 50 is regulated by PKAAnjaparavanda P Naren
Department of Physiology and Biophysics, Medical Center, University of Tennessee Health Science Center, Memphis 38163, USA
Proc Natl Acad Sci U S A 100:342-6. 2003..Deleting the regulatory domain of CFTR abolishes PKA regulation of complex assembly. This report summarizes a macromolecular signaling complex involving CFTR, the implications of which may be relevant to CFTR-dysfunction diseases... - The interaction between syntaxin 1A and cystic fibrosis transmembrane conductance regulator Cl- channels is mechanistically distinct from syntaxin 1A-SNARE interactionsRadhika Ganeshan
Department of Physiology and Biophysics, Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
J Biol Chem 278:2876-85. 2003..Thus, CFTR channel regulation by syntaxin 1A involves hydrophilic interactions that are mechanistically distinct from the hydrophobic interactions that mediate SNARE complex formation and Ca(2+) channel regulation by this t-SNARE... - CFTR chloride channels are regulated by a SNAP-23/syntaxin 1A complexEstelle Cormet-Boyaka
Department of Physiology and Biophysics, Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama, Birmingham, AL 35294, USA
Proc Natl Acad Sci U S A 99:12477-82. 2002..We conclude that CFTR channels are regulated by a t-SNARE complex that may tune CFTR activity to rates of membrane traffic in epithelial cells... - Coordinate regulation of catecholamine uptake by rab3 and phosphoinositide 3-kinaseSharon C Francis
Department of Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
J Biol Chem 277:7816-23. 2002..Our results indicate that rab3 and PI3K positively and coordinately regulate NE uptake in PC12 neuroendocrine cells at least in part by stimulating the secretory vesicle uptake step...
Research Grants
- REGULATION OF CFTR BY SYNTAXIN AND N-SEC 1 ISOFORMSKevin Kirk; Fiscal Year: 2005..g., protein secretion). Such information may lead to new strategies for manipulating CFTR function in cystic fibrosis and secretory diarrhea. ..
- NEW PARADIGMS OF CFTR REGULATIONKevin Kirk; Fiscal Year: 2007..The results of this project should help clarify the normal mechanisms that control CFTR gating, define the effect of the most common CF mutation on CFTR gating, and possibly lead to the development of new CF drugs. ..
- REGULATION OF CFTR BY SYNTAXIN AND N-SEC 1 ISOFORMSKevin Kirk; Fiscal Year: 2007..The results should establish the biologic roles of STX1A/1B in vivo and the physiologic significance of their interactions with the CFTR channel. ..
- REGULATION OF CFTR BY SYNTAXIN AND N-SEC 1 ISOFORMSKevin Kirk; Fiscal Year: 2009..The results should establish the biologic roles of STX1A/1B in vivo and the physiologic significance of their interactions with the CFTR channel. ..
- NEW PARADIGMS OF CFTR REGULATIONKevin Kirk; Fiscal Year: 2004....
- REGULATION OF CFTR BY SYNTAXIN AND N-SEC 1 ISOFORMSKevin Kirk; Fiscal Year: 1999....
- REGULATION OF CFTR BY SYNTAXIN AND N-SEC 1 ISOFORMSKevin L Kirk; Fiscal Year: 2010..The results should establish the biologic roles of STX1A/1B in vivo and the physiologic significance of their interactions with the CFTR channel. ..