Kevin Kirk

Summary

Affiliation: University of Alabama at Birmingham
Country: USA

Publications

  1. ncbi request reprint Mechanisms of CFTR regulation by syntaxin 1A and PKA
    Steven Y Chang
    Department of Medicine, Section of Pulmonary and Critical Care Medicine, The University of Chicago Hospitals, 5841 S Maryland Avenue, MC 6026, Chicago, IL 60637, USA
    J Cell Sci 115:783-91. 2002
  2. pmc A unified view of cystic fibrosis transmembrane conductance regulator (CFTR) gating: combining the allosterism of a ligand-gated channel with the enzymatic activity of an ATP-binding cassette (ABC) transporter
    Kevin L Kirk
    Department of Physiology and Biophysics and the Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Biol Chem 286:12813-9. 2011
  3. pmc ATP-independent CFTR channel gating and allosteric modulation by phosphorylation
    Wei Wang
    Department of Physiology and Biophysics, University of Alabama, Birmingham, AL 35294, USA
    Proc Natl Acad Sci U S A 107:3888-93. 2010
  4. ncbi request reprint Curcumin opens cystic fibrosis transmembrane conductance regulator channels by a novel mechanism that requires neither ATP binding nor dimerization of the nucleotide-binding domains
    Wei Wang
    Department of Physiology and Biophysics and the Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Biol Chem 282:4533-44. 2007
  5. ncbi request reprint How to review a paper
    Dale J Benos
    Department of Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Adv Physiol Educ 27:47-52. 2003
  6. pmc Rescuing cystic fibrosis transmembrane conductance regulator (CFTR)-processing mutants by transcomplementation
    Estelle Cormet-Boyaka
    Department of Physiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Proc Natl Acad Sci U S A 101:8221-6. 2004
  7. ncbi request reprint Cystic fibrosis transmembrane conductance regulator trafficking is mediated by the COPI coat in epithelial cells
    Jessica Rennolds
    Division of Pulmonary, Critical Care, and Sleep Medicine, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 283:833-9. 2008
  8. pmc CFTR surface expression and chloride currents are decreased by inhibitors of N-WASP and actin polymerization
    Radhika Ganeshan
    Department of Physiology and Biophysics, Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, 1918 University Blvd, MCLM 985, Birmingham, AL 35294, USA
    Biochim Biophys Acta 1773:192-200. 2007
  9. ncbi request reprint Mutations in the amino terminus of the cystic fibrosis transmembrane conductance regulator enhance endocytosis
    Asta Jurkuvenaite
    Department of Cell Biology, University of Alabama at Birmingham, AL 35294, USA
    J Biol Chem 281:3329-34. 2006
  10. ncbi request reprint Activating cystic fibrosis transmembrane conductance regulator channels with pore blocker analogs
    Wei Wang
    Department of Physiology and Biophysics, Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama 35294 0005, USA
    J Biol Chem 280:23622-30. 2005

Research Grants

Collaborators

  • Wei Wang
  • Dale J Benos
  • Anjaparavanda P Naren
  • Michael W Quick
  • Zsuzsanna Bebok
  • John P Clancy
  • Estelle Cormet-Boyaka
  • Radhika Ganeshan
  • Anke Di
  • Deborah J Nelson
  • Steven Y Chang
  • Jessica Rennolds
  • Krzysztof Nowotarski
  • Asta Jurkuvenaite
  • Sharon C Francis
  • Lois Musgrove
  • Cristy Tower
  • Kevin Maloney
  • Lijuan Fan
  • Elizabeth Sztul
  • James F Collawn
  • Yao Li
  • Karoly Varga
  • Eric J Sorscher
  • Michael Jablonsky
  • Donald D Muccio
  • Patricia L Jackson
  • H Clive Palfrey
  • Carl Sunshine
  • Albert Tousson

Detail Information

Publications15

  1. ncbi request reprint Mechanisms of CFTR regulation by syntaxin 1A and PKA
    Steven Y Chang
    Department of Medicine, Section of Pulmonary and Critical Care Medicine, The University of Chicago Hospitals, 5841 S Maryland Avenue, MC 6026, Chicago, IL 60637, USA
    J Cell Sci 115:783-91. 2002
    ..We also show that PKA activation can enhance membrane trafficking in some epithelial cell types, and this is independent from CFTR activation or syntaxin 1A association...
  2. pmc A unified view of cystic fibrosis transmembrane conductance regulator (CFTR) gating: combining the allosterism of a ligand-gated channel with the enzymatic activity of an ATP-binding cassette (ABC) transporter
    Kevin L Kirk
    Department of Physiology and Biophysics and the Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Biol Chem 286:12813-9. 2011
    ..g. unliganded openings and constitutive mutations). Here, we provide a unified view of CFTR gating that combines the allosterism of a ligand-gated channel with its unique enzymatic activity...
  3. pmc ATP-independent CFTR channel gating and allosteric modulation by phosphorylation
    Wei Wang
    Department of Physiology and Biophysics, University of Alabama, Birmingham, AL 35294, USA
    Proc Natl Acad Sci U S A 107:3888-93. 2010
    ..Our results confirm that (i) CFTR gating exhibits features of protein allostery that are shared with conventional ligand-gated channels and (ii) the R domain modulates CFTR activity independent of ATP-induced NBD dimerization...
  4. ncbi request reprint Curcumin opens cystic fibrosis transmembrane conductance regulator channels by a novel mechanism that requires neither ATP binding nor dimerization of the nucleotide-binding domains
    Wei Wang
    Department of Physiology and Biophysics and the Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Biol Chem 282:4533-44. 2007
    ..The phosphorylation dependence of curcumin activation indicates that the R domain can modulate channel opening without affecting ATP binding to the NBDs or their heterodimerization...
  5. ncbi request reprint How to review a paper
    Dale J Benos
    Department of Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Adv Physiol Educ 27:47-52. 2003
    ..Proper reviewer conduct is essential for making the peer review process valuable and the journal trustworthy...
  6. pmc Rescuing cystic fibrosis transmembrane conductance regulator (CFTR)-processing mutants by transcomplementation
    Estelle Cormet-Boyaka
    Department of Physiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Proc Natl Acad Sci U S A 101:8221-6. 2004
    ..These findings indicate that it may be possible to develop CF therapies (e.g., mini-cDNA constructs for gene therapy) that are tailored to specific disease-causing mutants of CFTR...
  7. ncbi request reprint Cystic fibrosis transmembrane conductance regulator trafficking is mediated by the COPI coat in epithelial cells
    Jessica Rennolds
    Division of Pulmonary, Critical Care, and Sleep Medicine, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 283:833-9. 2008
    ..We discovered that the alpha-, beta-, and gamma-subunits of COPI co-immunoprecipitated with CFTR. Our results indicate that the COPI complex plays a critical role in CFTR trafficking to the PM...
  8. pmc CFTR surface expression and chloride currents are decreased by inhibitors of N-WASP and actin polymerization
    Radhika Ganeshan
    Department of Physiology and Biophysics, Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, 1918 University Blvd, MCLM 985, Birmingham, AL 35294, USA
    Biochim Biophys Acta 1773:192-200. 2007
    ..Our studies implicate a role for N-WASP-mediated actin polymerization in regulating CFTR surface expression and channel activity...
  9. ncbi request reprint Mutations in the amino terminus of the cystic fibrosis transmembrane conductance regulator enhance endocytosis
    Asta Jurkuvenaite
    Department of Cell Biology, University of Alabama at Birmingham, AL 35294, USA
    J Biol Chem 281:3329-34. 2006
    ..These two additive effects contribute to the loss of surface expression and the associated defect in chloride conductance that is consistent with a disease phenotype...
  10. ncbi request reprint Activating cystic fibrosis transmembrane conductance regulator channels with pore blocker analogs
    Wei Wang
    Department of Physiology and Biophysics, Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama 35294 0005, USA
    J Biol Chem 280:23622-30. 2005
    ....
  11. pmc Reversible silencing of CFTR chloride channels by glutathionylation
    Wei Wang
    Department of Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Gen Physiol 125:127-41. 2005
    ..Our results demonstrate that human CFTR channels are reversibly inhibited by reactive glutathione species, and support an important role of the region proximal to the NBD2 signature sequence in ATP-dependent channel opening...
  12. pmc A macromolecular complex of beta 2 adrenergic receptor, CFTR, and ezrin/radixin/moesin-binding phosphoprotein 50 is regulated by PKA
    Anjaparavanda P Naren
    Department of Physiology and Biophysics, Medical Center, University of Tennessee Health Science Center, Memphis 38163, USA
    Proc Natl Acad Sci U S A 100:342-6. 2003
    ..Deleting the regulatory domain of CFTR abolishes PKA regulation of complex assembly. This report summarizes a macromolecular signaling complex involving CFTR, the implications of which may be relevant to CFTR-dysfunction diseases...
  13. ncbi request reprint The interaction between syntaxin 1A and cystic fibrosis transmembrane conductance regulator Cl- channels is mechanistically distinct from syntaxin 1A-SNARE interactions
    Radhika Ganeshan
    Department of Physiology and Biophysics, Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Biol Chem 278:2876-85. 2003
    ..Thus, CFTR channel regulation by syntaxin 1A involves hydrophilic interactions that are mechanistically distinct from the hydrophobic interactions that mediate SNARE complex formation and Ca(2+) channel regulation by this t-SNARE...
  14. pmc CFTR chloride channels are regulated by a SNAP-23/syntaxin 1A complex
    Estelle Cormet-Boyaka
    Department of Physiology and Biophysics, Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama, Birmingham, AL 35294, USA
    Proc Natl Acad Sci U S A 99:12477-82. 2002
    ..We conclude that CFTR channels are regulated by a t-SNARE complex that may tune CFTR activity to rates of membrane traffic in epithelial cells...
  15. ncbi request reprint Coordinate regulation of catecholamine uptake by rab3 and phosphoinositide 3-kinase
    Sharon C Francis
    Department of Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Biol Chem 277:7816-23. 2002
    ..Our results indicate that rab3 and PI3K positively and coordinately regulate NE uptake in PC12 neuroendocrine cells at least in part by stimulating the secretory vesicle uptake step...

Research Grants21

  1. REGULATION OF CFTR BY SYNTAXIN AND N-SEC 1 ISOFORMS
    Kevin Kirk; Fiscal Year: 2005
    ..g., protein secretion). Such information may lead to new strategies for manipulating CFTR function in cystic fibrosis and secretory diarrhea. ..
  2. NEW PARADIGMS OF CFTR REGULATION
    Kevin Kirk; Fiscal Year: 2007
    ..The results of this project should help clarify the normal mechanisms that control CFTR gating, define the effect of the most common CF mutation on CFTR gating, and possibly lead to the development of new CF drugs. ..
  3. REGULATION OF CFTR BY SYNTAXIN AND N-SEC 1 ISOFORMS
    Kevin Kirk; Fiscal Year: 2007
    ..The results should establish the biologic roles of STX1A/1B in vivo and the physiologic significance of their interactions with the CFTR channel. ..
  4. REGULATION OF CFTR BY SYNTAXIN AND N-SEC 1 ISOFORMS
    Kevin Kirk; Fiscal Year: 2009
    ..The results should establish the biologic roles of STX1A/1B in vivo and the physiologic significance of their interactions with the CFTR channel. ..
  5. NEW PARADIGMS OF CFTR REGULATION
    Kevin Kirk; Fiscal Year: 2004
    ....
  6. REGULATION OF CFTR BY SYNTAXIN AND N-SEC 1 ISOFORMS
    Kevin Kirk; Fiscal Year: 1999
    ....
  7. REGULATION OF CFTR BY SYNTAXIN AND N-SEC 1 ISOFORMS
    Kevin L Kirk; Fiscal Year: 2010
    ..The results should establish the biologic roles of STX1A/1B in vivo and the physiologic significance of their interactions with the CFTR channel. ..