S M King

Summary

Affiliation: University of Connecticut Health Center
Country: USA

Publications

  1. ncbi request reprint Dyneins motor on in plants
    Stephen M King
    Department of Biochemistry, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 06030 3305, USA
    Traffic 3:930-1. 2002
  2. ncbi request reprint Axonemal protofilament ribbons, DM10 domains, and the link to juvenile myoclonic epilepsy
    Stephen M King
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut, USA
    Cell Motil Cytoskeleton 63:245-53. 2006
  3. ncbi request reprint AAA domains and organization of the dynein motor unit
    S M King
    Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032 3305, USA
    J Cell Sci 113:2521-6. 2000
  4. ncbi request reprint Cytoplasmic dynein contains a family of differentially expressed light chains
    S M King
    Department of Biochemistry, Center for Biomedical Imaging Technology, University of Connecticut Health Center, Farmington 06032 3305, USA
    Biochemistry 37:15033-41. 1998
  5. ncbi request reprint The Tctex1/Tctex2 class of dynein light chains. Dimerization, differential expression, and interaction with the LC8 protein family
    L M DiBella
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06030 3305, USA
    J Biol Chem 276:14366-73. 2001
  6. ncbi request reprint Dynein motors of the Chlamydomonas flagellum
    L M DiBella
    Department of Biochemistry, University of Connecticut Health Center, Farmington 06032, USA
    Int Rev Cytol 210:227-68. 2001
  7. ncbi request reprint The mouse t-complex-encoded protein Tctex-1 is a light chain of brain cytoplasmic dynein
    S M King
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032 3305, USA
    J Biol Chem 271:32281-7. 1996
  8. pmc Chlamydomonas outer arm dynein alters conformation in response to Ca2+
    Miho Sakato
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
    Mol Biol Cell 18:3620-34. 2007
  9. ncbi request reprint Chlamydomonas FAP133 is a dynein intermediate chain associated with the retrograde intraflagellar transport motor
    Panteleimon Rompolas
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
    J Cell Sci 120:3653-65. 2007
  10. ncbi request reprint Calcium regulates ATP-sensitive microtubule binding by Chlamydomonas outer arm dynein
    Miho Sakato
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06030 3305, USA
    J Biol Chem 278:43571-9. 2003

Collaborators

Detail Information

Publications44

  1. ncbi request reprint Dyneins motor on in plants
    Stephen M King
    Department of Biochemistry, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 06030 3305, USA
    Traffic 3:930-1. 2002
    ..These observations also suggest that, in contrast to an earlier proposal, flowering plants may indeed use standard dynein-driven mechanisms to perform cellular transport activities that, in other organisms, employ the dynein motor..
  2. ncbi request reprint Axonemal protofilament ribbons, DM10 domains, and the link to juvenile myoclonic epilepsy
    Stephen M King
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut, USA
    Cell Motil Cytoskeleton 63:245-53. 2006
    ..Here I discuss recent work that supports assignment of human Efhc1 as a ciliary component and the resulting implications for the mechanism of disease causation...
  3. ncbi request reprint AAA domains and organization of the dynein motor unit
    S M King
    Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032 3305, USA
    J Cell Sci 113:2521-6. 2000
    ..The resulting structural parallels allow intriguing predictions to be made concerning dynein organization and motor function...
  4. ncbi request reprint Cytoplasmic dynein contains a family of differentially expressed light chains
    S M King
    Department of Biochemistry, Center for Biomedical Imaging Technology, University of Connecticut Health Center, Farmington 06032 3305, USA
    Biochemistry 37:15033-41. 1998
    ..Dissection of the cytoplasmic dynein complex revealed that Tctex1, an Mr 8000 LC dimer, and IC74 associate to define a basal-located intermediate chain/light chain complex analogous to that found in flagellar outer arm dynein...
  5. ncbi request reprint The Tctex1/Tctex2 class of dynein light chains. Dimerization, differential expression, and interaction with the LC8 protein family
    L M DiBella
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06030 3305, USA
    J Biol Chem 276:14366-73. 2001
    ..These data provide further support for the existence of functionally distinct populations of cytoplasmic dynein with differing light chain content...
  6. ncbi request reprint Dynein motors of the Chlamydomonas flagellum
    L M DiBella
    Department of Biochemistry, University of Connecticut Health Center, Farmington 06032, USA
    Int Rev Cytol 210:227-68. 2001
    ..The combination of genetic, physiological, structural, and biochemical approaches has made the Chlamydomonas flagellum a very powerful model system in which to dissect the function of these fascinating molecular motors...
  7. ncbi request reprint The mouse t-complex-encoded protein Tctex-1 is a light chain of brain cytoplasmic dynein
    S M King
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032 3305, USA
    J Biol Chem 271:32281-7. 1996
    ....
  8. pmc Chlamydomonas outer arm dynein alters conformation in response to Ca2+
    Miho Sakato
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
    Mol Biol Cell 18:3620-34. 2007
    ....
  9. ncbi request reprint Chlamydomonas FAP133 is a dynein intermediate chain associated with the retrograde intraflagellar transport motor
    Panteleimon Rompolas
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
    J Cell Sci 120:3653-65. 2007
    ..Furthermore, we found that FAP133, LC8, DHC1b, D1bLIC, the FLA10 kinesin-2 necessary for anterograde IFT and other IFT scaffold components associate to form a large macromolecular assembly...
  10. ncbi request reprint Calcium regulates ATP-sensitive microtubule binding by Chlamydomonas outer arm dynein
    Miho Sakato
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06030 3305, USA
    J Biol Chem 278:43571-9. 2003
    ....
  11. ncbi request reprint Organization and regulation of the dynein microtubule motor
    Stephen M King
    Department of Biochemistry, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 3305, USA
    Cell Biol Int 27:213-5. 2003
  12. doi request reprint Dynein-independent functions of DYNLL1/LC8: redox state sensing and transcriptional control
    Stephen M King
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    Sci Signal 1:pe51. 2008
    ..A recent report now proposes that DYNLL/LC8-driven interactions are also regulated by changes in cellular redox state, which lead to intermonomer disulfide bond formation and ultimately activation of the transcription factor NF-kappaB...
  13. pmc Modulation of Chlamydomonas reinhardtii flagellar motility by redox poise
    Ken Ichi Wakabayashi
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
    J Cell Biol 173:743-54. 2006
    ..These data indicate that redox poise directly affects ODAs and suggest that it may act in the control of flagellar motility...
  14. ncbi request reprint Redox-based control of the gamma heavy chain ATPase from Chlamydomonas outer arm dynein
    Alistair Harrison
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06030 3305, USA
    Cell Motil Cytoskeleton 52:131-43. 2002
    ..These data suggest the possibility of a dynein-associated redox cascade and further support the idea that the gamma heavy chain plays a key regulatory role within the outer arm...
  15. pmc Sensing the mechanical state of the axoneme and integration of Ca2+ signaling by outer arm dynein
    Stephen M King
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut 06030 3305, USA
    Cytoskeleton (Hoboken) 67:207-13. 2010
    ....
  16. pmc Flagellar radial spokes contain a Ca2+-stimulated nucleoside diphosphate kinase
    Ramila S Patel-King
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut 06030 3305, USA
    Mol Biol Cell 15:3891-902. 2004
    ..These observations suggest that nucleotides other than ATP may play an important role in the signal transduction pathway that underlies the regulatory mechanism defined by the radial spokes...
  17. ncbi request reprint Brain cytoplasmic and flagellar outer arm dyneins share a highly conserved Mr 8,000 light chain
    S M King
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032 3305, USA
    J Biol Chem 271:19358-66. 1996
    ..Thus, it appears that flagellar outer arm and brain cytoplasmic dyneins share a highly conserved light chain polypeptide that, at least in Drosophila, is essential for viability...
  18. ncbi request reprint The dynein microtubule motor
    S M King
    Department of Biochemistry, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06032 3305, USA
    Biochim Biophys Acta 1496:60-75. 2000
    ..Recent molecular, genetic and structural studies have revealed insight into the roles played by these various components and the mechanisms of dynein-based motility...
  19. pmc The LC7 light chains of Chlamydomonas flagellar dyneins interact with components required for both motor assembly and regulation
    Linda M DiBella
    Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    Mol Biol Cell 15:4633-46. 2004
    ..These data suggest that LC7a stabilizes both the outer arms and inner arm I1 and that both LC7a and LC7b are involved in multiple intradynein interactions within both dyneins...
  20. ncbi request reprint The Roadblock light chains are ubiquitous components of cytoplasmic dynein that form homo- and heterodimers
    Karina Nikulina
    Department of Biochemistry, University of Connecticut Health Center, Farmington 06030 3305, USA
    Cell Motil Cytoskeleton 57:233-45. 2004
    ..Immunofluorescence analysis reveals that Robl is present in punctate organelles in rat neuroblastoma cells. In testis, Robl is found in Leydig cells, spermatocytes, and sperm flagella...
  21. ncbi request reprint Relaxation-based structure refinement and backbone molecular dynamics of the dynein motor domain-associated light chain
    Hongwei Wu
    Department of Biochemistry, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 06030 3305, USA
    Biochemistry 42:57-71. 2003
    ..These results, which were cross validated using the 500 MHz data set, strongly support identification of the predicted LC1 binding surfaces and provide additional insight into the interaction mechanisms of leucine-rich repeat proteins...
  22. pmc An outer arm dynein light chain acts in a conformational switch for flagellar motility
    Ramila S Patel-King
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
    J Cell Biol 186:283-95. 2009
    ..We propose that this gamma HC-LC1-microtubule ternary complex functions as a conformational switch to control outer arm activity...
  23. pmc Differential light chain assembly influences outer arm dynein motor function
    Linda M DiBella
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    Mol Biol Cell 16:5661-74. 2005
    ..These data suggest that LC2, LC6, and LC9 have different roles in outer arm assembly and are required for wild-type motor function in the Chlamydomonas flagellum...
  24. ncbi request reprint A novel Tctex2-related light chain is required for stability of inner dynein arm I1 and motor function in the Chlamydomonas flagellum
    Linda M DiBella
    Department of Biochemistry, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 3305, USA
    J Biol Chem 279:21666-76. 2004
    ..Furthermore, using a microtubule sliding assay we observed that dynein motor function is reduced in vitro. These data indicate that Tctex2b is required for the stability of inner dynein arm I1 and wild-type axonemal dynein function...
  25. pmc Three members of the LC8/DYNLL family are required for outer arm dynein motor function
    Christopher A Tanner
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    Mol Biol Cell 19:3724-34. 2008
    ..g., oda1). These results support a key regulatory role for the intermediate chain/light chain complex that is an integral and highly conserved feature of all oligomeric dynein motors...
  26. ncbi request reprint A bipartite Ca2+-regulated nucleoside-diphosphate kinase system within the Chlamydomonas flagellum. The regulatory subunit p72
    Ramila S Patel-King
    Department of Biochemistry, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 3305, USA
    J Biol Chem 277:34271-9. 2002
    ..We propose that Ca(2+) regulation of flagellar motility in Chlamydomonas may be achieved in part through a downstream GTP-mediated signaling pathway...
  27. ncbi request reprint Backbone dynamics of dynein light chains
    Hongwei Wu
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06030 3305, USA
    Cell Motil Cytoskeleton 54:267-73. 2003
  28. ncbi request reprint Solution structure of the Tctex1 dimer reveals a mechanism for dynein-cargo interactions
    Hongwei Wu
    Department of Molecular, Microbial, and Structural Biology, 263 Farmington Avenue, Farmington, Connecticut 06030, USA
    Structure 13:213-23. 2005
    ..In addition, this structure provides insight into the consequences of the mutations found in the t haplotype forms of this protein...
  29. doi request reprint Purification of axonemal dyneins and dynein-associated components from Chlamydomonas
    Stephen M King
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, 06030 3305, USA
    Methods Cell Biol 92:31-48. 2009
    ..When combined with the wide range of available mutants, these methods allow for the analysis of dyneins lacking individual components or motor units...
  30. ncbi request reprint Design and regulation of the AAA+ microtubule motor dynein
    Miho Sakato
    Department of Biochemistry, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 3305, USA
    J Struct Biol 146:58-71. 2004
    ..Here we describe the two basic dynein designs and detail the various regulatory systems that impinge on this motor within the eukaryotic flagellum...
  31. doi request reprint Schmidtea mediterranea: a model system for analysis of motile cilia
    Panteleimon Rompolas
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut 06030 3305, USA
    Methods Cell Biol 93:81-98. 2009
    ..mediterranea. Overall, S. mediterranea is a highly versatile, easy to maintain, and genetically tractable organism that provides a powerful alternative model system for the study of motile cilia...
  32. ncbi request reprint The lissencephaly protein Lis1 is present in motile mammalian cilia and requires outer arm dynein for targeting to Chlamydomonas flagella
    Lotte B Pedersen
    Department of Molecular Biology, University of Copenhagen, Universitetsparken 13, DK 2100 Copenhagen OE, Denmark
    J Cell Sci 120:858-67. 2007
    ..Our results suggest that Lis1 and NudC are present in cilia and flagella and may regulate outer dynein arm activity...
  33. pmc Genetic analysis of the cytoplasmic dynein subunit families
    K Kevin Pfister
    Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville, Virginia, USA
    PLoS Genet 2:e1. 2006
    ....
  34. pmc Radial spoke proteins of Chlamydomonas flagella
    Pinfen Yang
    Department of Biological Sciences, Marquette University, Milwaukee, WI 53233, USA
    J Cell Sci 119:1165-74. 2006
    ..The human genes encoding these proteins are candidates for causing primary ciliary dyskinesia, a severe inherited disease involving missing or defective axonemal structures, including the radial spokes...
  35. pmc Partially functional outer-arm dynein in a novel Chlamydomonas mutant expressing a truncated gamma heavy chain
    Zhongmei Liu
    Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Eukaryot Cell 7:1136-45. 2008
    ..The availability of mutants lacking individual heavy chains should greatly facilitate studies on the structure and function of the outer-arm dynein...
  36. ncbi request reprint Protein modification to probe intradynein interactions and in vivo redox state
    Ken Ichi Wakabayashi
    Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo, Japan
    Methods Mol Biol 392:71-83. 2007
    ..Although designed for analysis of Chlamydomonas flagellar dyneins, these approaches should be readily adaptable to the study of other systems...
  37. ncbi request reprint Molecular characterization of Ciona sperm outer arm dynein reveals multiple components related to outer arm docking complex protein 2
    Akiko Hozumi
    Shimoda Marine Research Center, University of Tsukuba, Shizuoka, Japan
    Cell Motil Cytoskeleton 63:591-603. 2006
    ..Thus, the assembly of outer arm dynein onto doublet microtubules involves multiple coiled-coil proteins related to DC2...
  38. ncbi request reprint Identification and analysis of axonemal dynein light chain 1 in primary ciliary dyskinesia patients
    Judit Horvath
    Department of Pediatrics and Adolescent Medicine, Albert Ludwigs University, Freiburg, Germany
    Am J Respir Cell Mol Biol 33:41-7. 2005
    ..Based on these findings, we considered DNAL1 a candidate for PCD and sequenced all exons of DNAL1 in 86 patients. Mutational analysis was negative, excluding a major role of DNAL1 in the pathogenesis of PCD...
  39. ncbi request reprint Interaction of the DYNLT (TCTEX1/RP3) light chains and the intermediate chains reveals novel intersubunit regulation during assembly of the dynein complex
    Kevin W H Lo
    Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville, Virginia 22908, USA
    J Biol Chem 282:36871-8. 2007
    ..Thus, whereas all combinations of DYNLT light chain dimers can be made, not all of the possible combinations of the isoforms are utilized during the assembly of the dynein complex...
  40. ncbi request reprint Rab6 family proteins interact with the dynein light chain protein DYNLRB1
    Bas Wanschers
    Department of Cell Biology, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Cell Motil Cytoskeleton 65:183-96. 2008
    ..DYNLRB is a putative modulator of the intrinsic GTPase activity of GTP-binding proteins. In vitro we were not able to reproduce this effect on Rab6 GTPase activity...
  41. ncbi request reprint Land of the Rising Chlamy: a report of the 11th international meeting on the Cell and Molecular Biology of Chlamydomonas held in Kobe, Japan, from May 11--15, 2004
    Olivier Vallon
    Protist 155:373-80. 2004
  42. ncbi request reprint Testis-specific human small heat shock protein HSPB9 is a cancer/testis antigen, and potentially interacts with the dynein subunit TCTEL1
    Nicole J W de Wit
    Department of Pathology, University Medical Centre Nijmegen, 6500 HB Nijmegen, The Netherlands
    Eur J Cell Biol 83:337-45. 2004
    ..Additionally, immunohistochemical staining showed co-expression of HSPB9 and TCTEL1 in similar stages of spermatogenesis and in tumour cells. The possible functional significance of this interaction is discussed...
  43. ncbi request reprint The roadblock light chain binds a novel region of the cytoplasmic Dynein intermediate chain
    Stephen J Susalka
    Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    J Biol Chem 277:32939-46. 2002
    ..In addition, this roadblock-binding domain is significantly downstream from both the Tctex1- and LC8-binding sites, supporting the hypothesis that multiple light chain family members can bind to the same intermediate chain...
  44. pmc Cytoplasmic dynein nomenclature
    K Kevin Pfister
    Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    J Cell Biol 171:411-3. 2005
    ..This nomenclature recognizes the two distinct cytoplasmic dynein complexes and has the flexibility to accommodate the discovery of new subunits and isoforms...