Nicole King

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Insights into the origin of metazoan filopodia and microvilli
    Arnau Sebé-Pedrós
    Institut de Biologia Evolutiva CSIC Universitat Pompeu Fabra, Barcelona, Catalonia, Spain
    Mol Biol Evol 30:2013-23. 2013
  2. doi request reprint The genomic and cellular foundations of animal origins
    Daniel J Richter
    Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3200 email
    Annu Rev Genet 47:509-37. 2013
  3. pmc The genome of the choanoflagellate Monosiga brevicollis and the origin of metazoans
    Nicole King
    Department of Molecular and Cell Biology and the Center for Integrative Genomics, University of California, Berkeley, California 94720, USA
    Nature 451:783-8. 2008
  4. doi request reprint The premetazoan ancestry of cadherins
    Monika Abedin
    Department of Molecular and Cell Biology and Center for Integrative Genomics, University of California at Berkeley, Berkeley, CA 94720, USA
    Science 319:946-8. 2008
  5. pmc Algoriphagus machipongonensis sp. nov., co-isolated with a colonial choanoflagellate
    Rosanna A Alegado
    Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA 94720, USA
    Int J Syst Evol Microbiol 63:163-8. 2013
  6. ncbi request reprint The unicellular ancestry of animal development
    Nicole King
    Department of Molecular and Cell Biology, University of California, Berkeley, 142 Life Sciences Addition, 3200, 94720, USA
    Dev Cell 7:313-25. 2004
  7. pmc Premetazoan ancestry of the Myc-Max network
    Susan L Young
    Department of Molecular and Cell Biology, Division of Genetics, Genomics and Development, University of California, Berkeley, CA, USA
    Mol Biol Evol 28:2961-71. 2011
  8. ncbi request reprint Isolation of single choanoflagellate cells from field samples and establishment of clonal cultures
    Nicole King
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
    CSH Protoc 2009:pdb.prot5147. 2009
  9. ncbi request reprint Separation of choanoflagellate and bacterial genomic DNA
    Nicole King
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
    CSH Protoc 2009:pdb.prot5154. 2009
  10. ncbi request reprint Long-term frozen storage of choanoflagellate cultures
    Nicole King
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
    CSH Protoc 2009:pdb.prot5149. 2009

Collaborators

Detail Information

Publications35

  1. pmc Insights into the origin of metazoan filopodia and microvilli
    Arnau Sebé-Pedrós
    Institut de Biologia Evolutiva CSIC Universitat Pompeu Fabra, Barcelona, Catalonia, Spain
    Mol Biol Evol 30:2013-23. 2013
    ....
  2. doi request reprint The genomic and cellular foundations of animal origins
    Daniel J Richter
    Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3200 email
    Annu Rev Genet 47:509-37. 2013
    ..The ancestral functions of these and other genes may eventually be revealed through studies of gene and genome function in early-branching animals and their closest non-animal relatives. ..
  3. pmc The genome of the choanoflagellate Monosiga brevicollis and the origin of metazoans
    Nicole King
    Department of Molecular and Cell Biology and the Center for Integrative Genomics, University of California, Berkeley, California 94720, USA
    Nature 451:783-8. 2008
    ..The completion of the M. brevicollis genome allows us to reconstruct with increasing resolution the genomic changes that accompanied the origin of metazoans...
  4. doi request reprint The premetazoan ancestry of cadherins
    Monika Abedin
    Department of Molecular and Cell Biology and Center for Integrative Genomics, University of California at Berkeley, Berkeley, CA 94720, USA
    Science 319:946-8. 2008
    ....
  5. pmc Algoriphagus machipongonensis sp. nov., co-isolated with a colonial choanoflagellate
    Rosanna A Alegado
    Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA 94720, USA
    Int J Syst Evol Microbiol 63:163-8. 2013
    ..nov. is proposed. The type strain is PR1(T) (= ATCC BAA-2233(T) = DSM 24695(T))...
  6. ncbi request reprint The unicellular ancestry of animal development
    Nicole King
    Department of Molecular and Cell Biology, University of California, Berkeley, 142 Life Sciences Addition, 3200, 94720, USA
    Dev Cell 7:313-25. 2004
    ..The protozoan perspective on animal origins promises to provide a valuable window into the distant past and into the cellular bases of animal development...
  7. pmc Premetazoan ancestry of the Myc-Max network
    Susan L Young
    Department of Molecular and Cell Biology, Division of Genetics, Genomics and Development, University of California, Berkeley, CA, USA
    Mol Biol Evol 28:2961-71. 2011
    ..Furthermore, core features of metazoan Myc function, including heterodimerization with Max, binding to E-box sequences in DNA, and localization to the nucleus, predate the origin of metazoans...
  8. ncbi request reprint Isolation of single choanoflagellate cells from field samples and establishment of clonal cultures
    Nicole King
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
    CSH Protoc 2009:pdb.prot5147. 2009
    ..For the latter technique, repeating the isolation procedure using higher dilutions is advised to ensure that only one cell is the basis of an established culture...
  9. ncbi request reprint Separation of choanoflagellate and bacterial genomic DNA
    Nicole King
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
    CSH Protoc 2009:pdb.prot5154. 2009
    ..The final DNA sample from this protocol is expected to be 90%-95% enriched for choanoflagellate genomic DNA and to provide an adequate template for genome sequencing projects...
  10. ncbi request reprint Long-term frozen storage of choanoflagellate cultures
    Nicole King
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
    CSH Protoc 2009:pdb.prot5149. 2009
    ..To prevent cross-contamination among vials stored in the liquid phase of a liquid nitrogen freezer, the use of CryoFlex tubing to fully seal the vials is encouraged...
  11. ncbi request reprint Rapid Preparation of Genomic DNA from Monosiga brevicollis and Other Choanoflagellates
    Nicole King
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
    CSH Protoc 2009:pdb.prot5152. 2009
    ..The resulting genomic DNA provides a suitable template for a variety of polymerase chain reaction (PCR) techniques, but is too impure for procedures that require very high-quality DNA such as Southern blotting...
  12. ncbi request reprint Preparation of Total RNA from Monosiga brevicollis and Other Choanoflagellates
    Nicole King
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
    CSH Protoc 2009:pdb.prot5151. 2009
    ..g., those using TRIzol) are sufficient for many purposes, the approach described here minimizes RNA degradation and provides a high-quality template for downstream protocols, including real-time polymerase chain reaction...
  13. ncbi request reprint Visualizing the Subcellular Localization of Actin, {beta}-Tubulin, and DNA in Monosiga brevicollis
    Nicole King
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
    CSH Protoc 2009:pdb.prot5150. 2009
    ..The central challenge of this protocol is to treat the cells gently so that they retain their ultrastructure and remain attached to the coverslips during processing...
  14. ncbi request reprint Starting and Maintaining Monosiga brevicollis Cultures
    Nicole King
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
    CSH Protoc 2009:pdb.prot5148. 2009
    ..These cultures can then be maintained and expanded in preparation for DNA or RNA isolation or cell biological assays...
  15. pmc Early evolution of animal cell signaling and adhesion genes
    Scott A Nichols
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 103:12451-6. 2006
    ..From these data, we infer that key signaling and adhesion genes were in place early in animal evolution, before the divergence of sponge and eumetazoan lineages...
  16. ncbi request reprint Preparation of High-Molecular-Weight Genomic DNA from Monosiga brevicollis and Other Choanoflagellates
    Nicole King
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
    CSH Protoc 2009:pdb.prot5153. 2009
    ..This protocol describes a simple genomic DNA isolation strategy that should be effective for almost all choanoflagellate species and will provide genomic templates suitable for polymerase chain reactions and Southern blot analysis...
  17. pmc Cell differentiation and morphogenesis in the colony-forming choanoflagellate Salpingoeca rosetta
    Mark J Dayel
    Department of Molecular and Cell Biology, Division of Genetics, Genomics, and Development, Center for Integrative Genomics, University of California, Berkeley, 94720, USA
    Dev Biol 357:73-82. 2011
    ..Together, these results help establish S. rosetta as a model system for studying simple multicellularity in choanoflagellates and provide an experimental framework for investigating the origin of animal multicellularity and development...
  18. pmc Diverse evolutionary paths to cell adhesion
    Monika Abedin
    Department of Molecular and Cell Biology, Division of Genetics, Genomics, and Development, University of California at Berkeley, Berkeley, California 94720 3200, USA
    Trends Cell Biol 20:734-42. 2010
    ..Understanding the unicellular ancestry of cell adhesion helps illuminate the basic cell biology of multicellular development in modern organisms...
  19. pmc A bacterial sulfonolipid triggers multicellular development in the closest living relatives of animals
    Rosanna A Alegado
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States
    elife 1:e00013. 2012
    ..DOI:http://dx.doi.org/10.7554/eLife.00013.001...
  20. ncbi request reprint The choanoflagellates: heterotrophic nanoflagellates and sister group of the metazoa
    Nicole King
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
    CSH Protoc 2009:pdb.emo116. 2009
    ..Because of the similarity in morphology and ecology shown across the order Choanoflagellida, the protocols mentioned in this article should be transferable to most choanoflagellate species...
  21. ncbi request reprint Choanoflagellates
    Nicole King
    Department of Integrative Biology, University of California, Berkeley, Berkeley, California 94720, USA
    Curr Biol 15:R113-4. 2005
  22. pmc Complete genome sequence of Algoriphagus sp. PR1, bacterial prey of a colony-forming choanoflagellate
    Rosanna A Alegado
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3200, USA
    J Bacteriol 193:1485-6. 2011
    ..Here we announce the complete genome sequence of Algoriphagus sp. PR1 and initial findings from its annotation...
  23. pmc Prey Capture and Phagocytosis in the Choanoflagellate Salpingoeca rosetta
    Mark J Dayel
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, United States of America
    PLoS ONE 9:e95577. 2014
    ..This study offers insight into the process of prey ingestion by S. rosetta, and provides a context within which to consider potential ecological differences between solitary cells and colonies in choanoflagellates. ..
  24. pmc Evidence for sex and recombination in the choanoflagellate Salpingoeca rosetta
    Tera C Levin
    Howard Hughes Medical Institute and the Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Curr Biol 23:2176-80. 2013
    ....
  25. ncbi request reprint Conflicting phylogenetic signals at the base of the metazoan tree
    Antonis Rokas
    Howard Hughes Medical Institute, University of Wisconsin Madison, 1525 Linden Drive, Madison, WI 53706 1596, USA
    Evol Dev 5:346-59. 2003
    ..These factors raise concerns about the ability to resolve the phylogenetic history of animals with molecular sequences. A consensus view of animal evolution may require investigations of genome-scale characters...
  26. ncbi request reprint The origins of multicellularity: a multi-taxon genome initiative
    Inaki Ruiz-Trillo
    Canadian Institute for Advanced Research, Program in Evolutionary Biology, Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada
    Trends Genet 23:113-8. 2007
    ..Here, we introduce this initiative and the biological questions that underpin it, summarize the rationale guiding the choice of organisms and discuss the anticipated benefits to the broader scientific community...
  27. ncbi request reprint Evolution of key cell signaling and adhesion protein families predates animal origins
    Nicole King
    Howard Hughes Medical Institute HHMI, University of Wisconsin, 1525 Linden Drive, Madison, WI 53706, USA
    Science 301:361-3. 2003
    ..The expression in choanoflagellates of proteins involved in cell interactions in Metazoa demonstrates that these proteins evolved before the origin of animals and were later co-opted for development...
  28. ncbi request reprint Genome-scale approaches to resolving incongruence in molecular phylogenies
    Antonis Rokas
    Howard Hughes Medical Institute, Laboratory of Molecular Biology, R M Bock Laboratories, University of Wisconsin Madison, 1525 Linden Drive, Madison, Wisconsin 53706, USA
    Nature 425:798-804. 2003
    ..These results have important implications for resolving branches of the tree of life...
  29. ncbi request reprint A linkage study between the GABAA beta2 and GABAA gamma2 subunit genes and major psychoses
    Alda M Ambrósio
    Unit of Clinical and Molecular Genetics, National Institute of Legal Medicine, Faculty of Medicine, University of Coimbra, Largo da Se Nova, 3000 213 Coimbra, Portugal
    CNS Spectr 10:57-61. 2005
    ..Alterations of the gamma-aminobutyric acid (GABA) system have been implicated in the pathophysiology of major psychoses...
  30. pmc Genetic dissection of the sporulation protein SpoIIE and its role in asymmetric division in Bacillus subtilis
    Karen Carniol
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    J Bacteriol 187:3511-20. 2005
    ..Additionally, we show that neither the phosphatase function of SpoIIE nor the N-terminal, membrane-spanning region is needed for the switch to asymmetric division...
  31. ncbi request reprint Association study of a novel functional polymorphism of the serotonin transporter gene in bipolar disorder and suicidal behaviour
    Vincenzo De Luca
    Neurogenetics Section, Clarke Site, Centre for Addiction and Mental Health, Department of Psychiatry, University of Toronto, 250 College Street R 30, Toronto, Ontario, Canada, M5T 1R8, Canada
    Psychopharmacology (Berl) 182:128-31. 2005
    ..84 df=4 P=0.76). These findings suggest that this new polymorphism in the 5-HTT gene may not influence suicidal behaviour in patients with bipolar disorder...
  32. pmc Signaling properties of a non-metazoan Src kinase and the evolutionary history of Src negative regulation
    Wanqing Li
    Department of Physiology and Biophysics, School of Medicine, Stony Brook University, Stony Brook, NY 11794, USA
    J Biol Chem 283:15491-501. 2008
    ..Thus, Src autoinhibition likely evolved more recently within the metazoan lineage, and it may have played a role in the establishment of intercellular signaling in metazoans...
  33. pmc Sterols in a unicellular relative of the metazoans
    Robin B Kodner
    Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 105:9897-902. 2008
    ..brevicollis display characteristics of both fungal and poriferan sterol biosynthesis. This is an example in which genomic and biochemical information have been used together to investigate the taxonomic specificity of a fossil biomarker...
  34. pmc Comparative biology and genomics join forces to decipher the diversity of life
    Nicole King
    Howard Hughes Medical Institute and University of Wisconsin, Madison, 1525 Linden Drive, Madison, WI 53706, USA
    Genome Biol 3:REPORTS4023. 2002
    ..A report on the Cold Spring Harbor Laboratory meeting on the Evolution of Developmental Diversity, Cold Spring Harbor, NY, USA, 17-21 April 2002...

Research Grants1

  1. Choanoflagellate colony formation as a simple model for animal multicellularity
    Nicole King; Fiscal Year: 2010
    ..By reconstructing the ancestry of intercellular interactions, this research aims to provide new insights into animal development, health and disease. ..