Research Topics
| Nicole KingSummaryAffiliation: University of California Country: USA Publications
Research Grants
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Detail Information
Publications
The genome of the choanoflagellate Monosiga brevicollis and the origin of metazoansNicole King
Department of Molecular and Cell Biology and the Center for Integrative Genomics, University of California, Berkeley, California 94720, USA
Nature 451:783-8. 2008..The completion of the M. brevicollis genome allows us to reconstruct with increasing resolution the genomic changes that accompanied the origin of metazoans...
The premetazoan ancestry of cadherinsMonika Abedin
Department of Molecular and Cell Biology and Center for Integrative Genomics, University of California at Berkeley, Berkeley, CA 94720, USA
Science 319:946-8. 2008....
Separation of choanoflagellate and bacterial genomic DNANicole King
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
CSH Protoc 2009:pdb.prot5154. 2009..The final DNA sample from this protocol is expected to be 90%-95% enriched for choanoflagellate genomic DNA and to provide an adequate template for genome sequencing projects...- Preparation of High-Molecular-Weight Genomic DNA from Monosiga brevicollis and Other ChoanoflagellatesNicole King
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
CSH Protoc 2009:pdb.prot5153. 2009..This protocol describes a simple genomic DNA isolation strategy that should be effective for almost all choanoflagellate species and will provide genomic templates suitable for polymerase chain reactions and Southern blot analysis... - Isolation of single choanoflagellate cells from field samples and establishment of clonal culturesNicole King
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
CSH Protoc 2009:pdb.prot5147. 2009..For the latter technique, repeating the isolation procedure using higher dilutions is advised to ensure that only one cell is the basis of an established culture... - Starting and Maintaining Monosiga brevicollis CulturesNicole King
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
CSH Protoc 2009:pdb.prot5148. 2009..These cultures can then be maintained and expanded in preparation for DNA or RNA isolation or cell biological assays... - Long-term frozen storage of choanoflagellate culturesNicole King
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
CSH Protoc 2009:pdb.prot5149. 2009..To prevent cross-contamination among vials stored in the liquid phase of a liquid nitrogen freezer, the use of CryoFlex tubing to fully seal the vials is encouraged... - Visualizing the Subcellular Localization of Actin, {beta}-Tubulin, and DNA in Monosiga brevicollisNicole King
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
CSH Protoc 2009:pdb.prot5150. 2009..The central challenge of this protocol is to treat the cells gently so that they retain their ultrastructure and remain attached to the coverslips during processing... - Preparation of Total RNA from Monosiga brevicollis and Other ChoanoflagellatesNicole King
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
CSH Protoc 2009:pdb.prot5151. 2009..g., those using TRIzol) are sufficient for many purposes, the approach described here minimizes RNA degradation and provides a high-quality template for downstream protocols, including real-time polymerase chain reaction... - Rapid Preparation of Genomic DNA from Monosiga brevicollis and Other ChoanoflagellatesNicole King
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
CSH Protoc 2009:pdb.prot5152. 2009..The resulting genomic DNA provides a suitable template for a variety of polymerase chain reaction (PCR) techniques, but is too impure for procedures that require very high-quality DNA such as Southern blotting... - Early evolution of animal cell signaling and adhesion genesScott A Nichols
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 103:12451-6. 2006..From these data, we infer that key signaling and adhesion genes were in place early in animal evolution, before the divergence of sponge and eumetazoan lineages... - The unicellular ancestry of animal developmentNicole King
Department of Molecular and Cell Biology, University of California, Berkeley, 142 Life Sciences Addition, 3200, 94720, USA
Dev Cell 7:313-25. 2004..The protozoan perspective on animal origins promises to provide a valuable window into the distant past and into the cellular bases of animal development... - Complete genome sequence of Algoriphagus sp. PR1, bacterial prey of a colony-forming choanoflagellateRosanna A Alegado
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3200, USA
J Bacteriol 193:1485-6. 2011..Here we announce the complete genome sequence of Algoriphagus sp. PR1 and initial findings from its annotation... - Premetazoan ancestry of the Myc-Max networkSusan L Young
Department of Molecular and Cell Biology, Division of Genetics, Genomics and Development, University of California, Berkeley, CA, USA
Mol Biol Evol 28:2961-71. 2011..Furthermore, core features of metazoan Myc function, including heterodimerization with Max, binding to E-box sequences in DNA, and localization to the nucleus, predate the origin of metazoans... - Diverse evolutionary paths to cell adhesionMonika Abedin
Department of Molecular and Cell Biology, Division of Genetics, Genomics, and Development, University of California at Berkeley, Berkeley, California 94720 3200, USA
Trends Cell Biol 20:734-42. 2010..Understanding the unicellular ancestry of cell adhesion helps illuminate the basic cell biology of multicellular development in modern organisms... - A bacterial sulfonolipid triggers multicellular development in the closest living relatives of animalsRosanna A Alegado
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States
elife 1:e00013. 2012..DOI:http://dx.doi.org/10.7554/eLife.00013.001... - The choanoflagellates: heterotrophic nanoflagellates and sister group of the metazoaNicole King
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
CSH Protoc 2009:pdb.emo116. 2009..Because of the similarity in morphology and ecology shown across the order Choanoflagellida, the protocols mentioned in this article should be transferable to most choanoflagellate species... - ChoanoflagellatesNicole King
Department of Integrative Biology, University of California, Berkeley, Berkeley, California 94720, USA
Curr Biol 15:R113-4. 2005 - Cell differentiation and morphogenesis in the colony-forming choanoflagellate Salpingoeca rosettaMark J Dayel
Department of Molecular and Cell Biology, Division of Genetics, Genomics, and Development, Center for Integrative Genomics, University of California, Berkeley, 94720, USA
Dev Biol 357:73-82. 2011..Together, these results help establish S. rosetta as a model system for studying simple multicellularity in choanoflagellates and provide an experimental framework for investigating the origin of animal multicellularity and development... - Evolution of key cell signaling and adhesion protein families predates animal originsNicole King
Howard Hughes Medical Institute (HHMI, University of Wisconsin, 1525 Linden Drive, Madison, WI 53706, USA
Science 301:361-3. 2003..The expression in choanoflagellates of proteins involved in cell interactions in Metazoa demonstrates that these proteins evolved before the origin of animals and were later co-opted for development... - Conflicting phylogenetic signals at the base of the metazoan treeAntonis Rokas
Howard Hughes Medical Institute, University of Wisconsin-Madison, 1525 Linden Drive, Madison, WI 53706-1596, USA
Evol Dev 5:346-59. 2003..These factors raise concerns about the ability to resolve the phylogenetic history of animals with molecular sequences. A consensus view of animal evolution may require investigations of genome-scale characters... - The origins of multicellularity: a multi-taxon genome initiativeIñaki Ruiz Trillo
Canadian Institute for Advanced Research, Program in Evolutionary Biology, Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada
Trends Genet 23:113-8. 2007..Here, we introduce this initiative and the biological questions that underpin it, summarize the rationale guiding the choice of organisms and discuss the anticipated benefits to the broader scientific community... - Sterols in a unicellular relative of the metazoansRobin B Kodner
Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA
Proc Natl Acad Sci U S A 105:9897-902. 2008..brevicollis display characteristics of both fungal and poriferan sterol biosynthesis. This is an example in which genomic and biochemical information have been used together to investigate the taxonomic specificity of a fossil biomarker... - Signaling properties of a non-metazoan Src kinase and the evolutionary history of Src negative regulationWanqing Li
Department of Physiology and Biophysics, School of Medicine, Stony Brook University, Stony Brook, NY 11794, USA
J Biol Chem 283:15491-501. 2008..Thus, Src autoinhibition likely evolved more recently within the metazoan lineage, and it may have played a role in the establishment of intercellular signaling in metazoans... - Association study of a novel functional polymorphism of the serotonin transporter gene in bipolar disorder and suicidal behaviourVincenzo De Luca
Neurogenetics Section, Clarke Site, Centre for Addiction and Mental Health, Department of Psychiatry, University of Toronto, 250 College Street R 30, Toronto, Ontario, Canada, M5T 1R8, Canada
Psychopharmacology (Berl) 182:128-31. 2005..84 df=4 P=0.76). These findings suggest that this new polymorphism in the 5-HTT gene may not influence suicidal behaviour in patients with bipolar disorder... - Genetic dissection of the sporulation protein SpoIIE and its role in asymmetric division in Bacillus subtilisKaren Carniol
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
J Bacteriol 187:3511-20. 2005..Additionally, we show that neither the phosphatase function of SpoIIE nor the N-terminal, membrane-spanning region is needed for the switch to asymmetric division... - A linkage study between the GABAA beta2 and GABAA gamma2 subunit genes and major psychosesAlda M Ambrósio
Unit of Clinical and Molecular Genetics, National Institute of Legal Medicine, Faculty of Medicine, University of Coimbra, Largo da Se Nova, 3000 213 Coimbra, Portugal
CNS Spectr 10:57-61. 2005..Alterations of the gamma-aminobutyric acid (GABA) system have been implicated in the pathophysiology of major psychoses... - Genome-scale approaches to resolving incongruence in molecular phylogeniesAntonis Rokas
Howard Hughes Medical Institute, Laboratory of Molecular Biology, R. M. Bock Laboratories, University of Wisconsin-Madison, 1525 Linden Drive, Madison, Wisconsin 53706, USA
Nature 425:798-804. 2003..These results have important implications for resolving branches of the tree of life... - Comparative biology and genomics join forces to decipher the diversity of lifeNicole King
Howard Hughes Medical Institute and University of Wisconsin, Madison, 1525 Linden Drive, Madison, WI 53706, USA
Genome Biol 3:REPORTS4023. 2002..A report on the Cold Spring Harbor Laboratory meeting on the Evolution of Developmental Diversity, Cold Spring Harbor, NY, USA, 17-21 April 2002...
Research Grants
- Choanoflagellate colony formation as a simple model for animal multicellularityNicole King; Fiscal Year: 2010..By reconstructing the ancestry of intercellular interactions, this research aims to provide new insights into animal development, health and disease. ..