Research Topics
| LAURA KIESSLINGSummaryAffiliation: University of Wisconsin Country: USA Publications
Research Grants
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Detail Information
Publications
Glycopolymer probes of signal transductionLaura L Kiessling
Department of Chemistry, University of Wisconsin Madison, 1101 University Ave, Madison, WI 53706, USA
Chem Soc Rev 42:4476-91. 2013..Our objective is to illustrate how these powerful tools can reveal the molecular mechanisms that underlie carbohydrate-mediated signal transduction...
Symbiosis: Chemical biology at WisconsinLaura L Kiessling
Department of Biochemistry and Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, Wisconsin 53706, USA
ACS Chem Biol 1:481-4. 2006
Synthetic multivalent ligands as probes of signal transductionLaura L Kiessling
Department of Chemistry, University of Wisconsin Madison, 1101 University Ave, Madison, WI 53706, USA
Angew Chem Int Ed Engl 45:2348-68. 2006..Multivalent ligands can be generated that possess a variety of sizes, shapes, valencies, orientations, and densities of binding elements. This Review focuses on the use of synthetic multivalent ligands to characterize receptor function...- A strategy for designing inhibitors of beta-amyloid toxicityJ Ghanta
Department of Chemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
J Biol Chem 271:29525-8. 1996..Significantly, these results demonstrate that complete disruption of amyloid fibril formation is not necessary for abrogation of toxicity... - Chemical approaches to glycobiologyLaura L Kiessling
Department of Chemistry, University of Wisconsin Madison, Wisconsin 53706, USA
Annu Rev Biochem 79:619-53. 2010..These examples illustrate how chemical glycobiology is providing new insight into the functional roles of glycans and new opportunities to interfere with or exploit these roles... - Hitting the sweet spotLaura L Kiessling
Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA
Nat Biotechnol 20:234-5. 2002 - Synthetic multivalent ligands in the exploration of cell-surface interactionsL L Kiessling
Departments of Chemistry and Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
Curr Opin Chem Biol 4:696-703. 2000.... - Transforming the cell surface through proteolysisL L Kiessling
Department of Chemistry, University of Wisconsin Madison 53706, USA
Chem Biol 5:R49-62. 1998..Growing evidence suggesting that protein shedding and protein function are closely linked may lead to new strategies for the treatment of a wide range of diseases... - Bifunctional ligands that target cells displaying the alpha v beta3 integrinRobert M Owen
Department of Chemistry, University of Wisconsin Madison, Madison, WI 53706, USA
Chembiochem 8:68-82. 2007..We anticipate that our modifiable alpha v beta3-binding ligands will be valuable in a variety of applications, including drug delivery and tumor targeting... - A defined glycosaminoglycan-binding substratum for human pluripotent stem cellsJoseph R Klim
Cell and Molecular Biology Program, University of Wisconsin Madison, Madison, Wisconsin, USA
Nat Methods 7:989-94. 2010..The peptides supported growth of eight pluripotent cell lines on a variety of scaffolds. Our results indicate that synthetic substrates that recognize cell-surface glycans can facilitate the long-term culture of pluripotent stem cells... - Chemical probes of bacterial signal transduction reveal that repellents stabilize and attractants destabilize the chemoreceptor arrayM Jack Borrok
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
ACS Chem Biol 3:101-9. 2008..Moreover, our data demonstrate that repellents can be transformed into attractants merely by their multivalent display. These results have implications for designing agonists and antagonists for other signaling systems... - Non-carbohydrate inhibitors of the lectin DC-SIGNM Jack Borrok
Department of Biochemistry and Chemistry, University of Wisconsin, Madison, WI 53706, USA
J Am Chem Soc 129:12780-5. 2007..Thus, we anticipate that these non-carbohydrate inhibitors can be used to illuminate the role of DC-SIGN in pathogenesis and immune function... - Synthetic glycoprotein mimics inhibit L-selectin-mediated rolling and promote L-selectin sheddingPatricia Mowery
Department of Biochemistry, 433 Babcock Drive, University of Wisconsin-Madison, Madison, WI 53706 USA
Chem Biol 11:725-32. 2004..Thus, their inhibitory potency may arise from their ability to induce shedding. Our data indicate that screening for compounds that promote L-selectin release can identify ligands that inhibit rolling... - Inter-receptor communication through arrays of bacterial chemoreceptorsJason E Gestwicki
Department of Chemistry, University of Wisconsin-Madison, 53706, USA
Nature 415:81-4. 2002..These results demonstrate that the entire array is involved in sensing. This mode of information exchange has general implications for the processing of signals by cellular receptors... - Conserved amplification of chemotactic responses through chemoreceptor interactionsAllison C Lamanna
Department of Biochemistry, University of Wisconsin at Madison, Madison, Wisconsin 53706, USA
J Bacteriol 184:4981-7. 2002..coli, signaling information is transferred among chemoreceptors in B. subtilis. These results suggest that interreceptor communication may be a general mechanism for modulating chemotactic responses in bacteria... - Influencing receptor-ligand binding mechanisms with multivalent ligand architectureJason E Gestwicki
Departments of Biochemistry and Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA
J Am Chem Soc 124:14922-33. 2002..Diversity-oriented syntheses of multivalent ligands coupled with effective assays that can be used to compare the contributions of different binding parameters may afford ligands that function by specific mechanisms... - A polymer scaffold for protein oligomerizationByron R Griffith
Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA
J Am Chem Soc 126:1608-9. 2004..We demonstrate that they can oligomerize fibroblast growth factor-8b (FGF-8b) and promote FGF-8b-mediated cell proliferation in the absence of heparin... - Potent ligands for prokaryotic UDP-galactopyranose mutase that exploit an enzyme subsiteEmily C Dykhuizen
Department of Chemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
Org Lett 11:193-6. 2009..The potency of these compounds stems from their ability to access both the substrate binding pocket and an adjacent site... - Synthesis and applications of end-labeled neoglycopolymersRobert M Owen
Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA
Org Lett 4:2293-6. 2002..The data reveal that these multivalent ligands interact with multiple copies of L-selectin... - Structure-based design of a periplasmic binding protein antagonist that prevents domain closureM Jack Borrok
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
ACS Chem Biol 4:447-56. 2009..These findings provide a blueprint for the design of new bacterial PBP inhibitors. Given the key role of PBPs in microbial physiology, we anticipate that PBP antagonists will have widespread uses as probes and antimicrobial agents... - Ligand binding and substrate discrimination by UDP-galactopyranose mutaseTodd D Gruber
Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706 1544, USA
J Mol Biol 391:327-40. 2009..To understand the consequences of these differences, we derived a model for the productive UGM-substrate complex that highlights interactions that can contribute to catalysis and substrate discrimination... - A tethering mechanism for length control in a processive carbohydrate polymerizationJohn F May
Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706, USA
Proc Natl Acad Sci U S A 106:11851-6. 2009..The strength of interaction of that tether with the polymerase influences the length of the resultant polymer. Thus, our data identify a mechanism for length control by a template-independent polymerase... - High-throughput discovery of synthetic surfaces that support proliferation of pluripotent cellsRatmir Derda
Department of Chemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
J Am Chem Soc 132:1289-95. 2010..Our results indicate that this screening strategy is a productive avenue for the generation of materials that control the growth and differentiation of cells... - Selective immobilization of multivalent ligands for surface plasmon resonance and fluorescence microscopyJason E Gestwicki
Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA
Anal Biochem 305:149-55. 2002..Together, our results indicate that synthetic surfaces can be created by selective immobilization of multivalent ligands and that these surfaces are capable of binding soluble and cell-surface-associated receptors with high affinity... - Synthesis of galactofuranose-based acceptor substrates for the study of the carbohydrate polymerase GlfT2Rebecca A Splain
Department of Chemistry, University of Wisconsin, Madison, WI 53706, USA
Bioorg Med Chem 18:3753-9. 2010..Moreover, our investigations indicate that lipids possessing but a single galactofuranose residue can act as substrates for GlfT2... - Unexpected enhancement in biological activity of a GPCR ligand induced by an oligoethylene glycol substituentChutima Jiarpinitnun
Department of Chemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
J Am Chem Soc 132:8844-5. 2010.... - A general glycomimetic strategy yields non-carbohydrate inhibitors of DC-SIGNKathleen C A Garber
Department of Chemistry, University of Wisconsin Madison, 1101 University Avenue, Madison, WI 53706, USA
Chem Commun (Camb) 46:6747-9. 2010..Shikimic acid can be transformed into monovalent and multivalent glycomimetics that target different members of the C-type lectin class, including DC-SIGN, a dendritic cell lectin that facilitates HIV transmission... - Synthesis of fluorogenic polymers for visualizing cellular internalizationShane L Mangold
Department of Chemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
Org Lett 10:2997-3000. 2008..The utility of ligands of this type is highlighted by visualizing multivalent antigen internalization in live B cells... - A polymeric domain that promotes cellular internalizationErin M Kolonko
Department of Chemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
J Am Chem Soc 130:5626-7. 2008..Because the synthesis of such materials is modular, we anticipate that compounds of this type can be fashioned that facilitate the delivery of cargo via end-cap derivatization or block copolymer synthesis... - Stereoselective N-glycosylation by Staudinger ligationYi He
Departments of Chemistry and Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA
Org Lett 6:4479-82. 2004..Our results provide precedence for the use of this powerful amide-bond-forming reaction for N-glycopeptide synthesis. [reaction: see text].. - Visualization and characterization of receptor clusters by transmission electron microscopyJason E Gestwicki
Departments of Chemistry and Biochemistry, University of Wisconsin, 1101 University Avenue, Madison, Wisconsin 53706, USA
Methods Enzymol 362:301-12. 2003 - Chemical probes of UDP-galactopyranose mutaseErin E Carlson
Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA
Chem Biol 13:825-37. 2006..Our studies offer a blueprint for identifying inhibitors of the growing family of UGM homologs and provide insight into UGM inhibition... - Synthesis of a multivalent display of a CD22-binding trisaccharideZhi-Qiang Yang
Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA
Carbohydr Res 337:1605-13. 2002..3. A fluorescein-labeled version of the multivalent ligand binds to cells expressing CD22... - Activating B cell signaling with defined multivalent ligandsErik B Puffer
Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
ACS Chem Biol 2:252-62. 2007..Our data suggest a link between the mechanisms underlying signal initiation by receptors that must respond with high sensitivity... - Conformational changes of glucose/galactose-binding protein illuminated by open, unliganded, and ultra-high-resolution ligand-bound structuresM Jack Borrok
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
Protein Sci 16:1032-41. 2007..Together, these structures provide insight into how the hinge-bending movement of GGBP facilitates ligand binding, transport, and signaling... - Defined substrates for human embryonic stem cell growth identified from surface arraysRatmir Derda
Department of Chemistry, 1101 University Avenue, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
ACS Chem Biol 2:347-55. 2007..Our results demonstrate that synthetic substrates for promoting and probing human ES cell self-renewal can be discovered through SAM surface arrays... - Sialylated multivalent antigens engage CD22 in trans and inhibit B cell activationAdam H Courtney
Departments of Biochemistry and Chemistry, University of Wisconsin, Madison, WI 53706, USA
Proc Natl Acad Sci U S A 106:2500-5. 2009..The ability of sialylated antigens to inhibit BCR signaling through trans CD22 interactions reveals a previously unrecognized role for the Siglec-family of receptors as modulators of immune signaling... - Fostering major breakthroughsLaura L Kiessling
ACS Chem Biol 1:1-2. 2006 - A higher degree of difficultyLaura L Kiessling
ACS Chem Biol 2:197-9. 2007 - Trophoblast L-selectin-mediated adhesion at the maternal-fetal interfaceOlga D Genbacev
Departments of Stomatology, Anatomy, and Pharmaceutical Chemistry, University of California, San Francisco, CA 94143, USA
Science 299:405-8. 2003..These results suggest that trophoblast L-selectin mediates interactions with the uterus and that this adhesion mechanism may be critical to establishing human pregnancy... - Link between chemotactic response to Ni2+ and its adsorption onto the Escherichia coli cell surfaceDavid Borrok
Department of Civil Engineering and Geological Sciences, University of Notre Dame, Notre Dame, Indiana 46556, USA
Environ Sci Technol 39:5227-33. 2005..Our data suggest that specified changes in environmental conditions can be used to tune chemotactic responses in natural biological and geological settings...
Research Grants
- Synthetic Ligands for Modulating B-Cell ResponsesLaura L Kiessling; Fiscal Year: 2010..g., for treating autoimmune diseases). The goal of this proposal is to synthesize new compounds that can be used to control B cell responses. ..
- The Chemistry and Biology of Galactofuranose ResiduesLaura L Kiessling; Fiscal Year: 2010..The goal of this project is to understand key steps in mycobacterial cell wall biosynthesis that are not targeted by any current drugs and find inhibitors from which new types of drugs could be developed. ..
- PROBES OF CARBOHYDRATE FUNCTIONLAURA KIESSLING; Fiscal Year: 2006..We anticipate that the results from our studies will provide new tools for the investigation of DC-SIGN and DC-SIGNR function in particular and for probing C-type lectin function in general. ..
- The Chemistry and Biology of Galactofuranose ResiduesLAURA KIESSLING; Fiscal Year: 2007..Moreover, we hope that by pursuing the proposed investigations, new leads for the treatment of tuberculosis may emerge. ..
- Chemical Biology Interface Training GrantLAURA KIESSLING; Fiscal Year: 2007..This program will continue to provide CBI trainees with unique opportunities that will prepare them for leadership roles in academia and industry. ..
- Mulitvalent Ligands as EffectorsLAURA KIESSLING; Fiscal Year: 2007..Moreover, neutrophil chemotaxis occurs in the inflammatory/immune response. Thus, conclusions from our studies will be broadly applicable to understanding other signaling pathways involved in human disease. ..
- Glycopeptides and Other Non-Natural Variants: Probes of Carbohydrate FunctionLAURA KIESSLING; Fiscal Year: 2009..This project is aimed at identifying DC-SIGN inhibitors and using compounds that bind to DC-SIGN to study its function. ..
- Synthetic Ligands for Modulating B-Cell ResponsesLAURA KIESSLING; Fiscal Year: 2009..g., for treating autoimmune diseases). The goal of this proposal is to synthesize new compounds that can be used to control B cell responses. ..
- Mulitvalent Ligands as EffectorsLAURA KIESSLING; Fiscal Year: 2009..Moreover, neutrophil chemotaxis occurs in the inflammatory/immune response. Thus, conclusions from our studies will be broadly applicable to understanding other signaling pathways involved in human disease. ..
- Glycopeptides and Other Non-Natural Variants: Probes of Carbohydrate FunctionLaura L Kiessling; Fiscal Year: 2010..This project is aimed at identifying DC-SIGN inhibitors and using compounds that bind to DC-SIGN to study its function. ..
- Glycopeptides and Other Non-Natural Variants: Probes of Carbohydrate FunctionLAURA KIESSLING; Fiscal Year: 2009..This project is aimed at identifying DC-SIGN inhibitors and using compounds that bind to DC-SIGN to study its function. ..
- Synthetic Ligands for Modulating B-Cell ResponsesLaura L Kiessling; Fiscal Year: 2011..g., for vaccine development), and those that block B cell activation could lead to tolerance (e.g., for treating autoimmune diseases). The goal of this proposal is to synthesize new compounds that can be used to control B cell responses. ..
- Synthetic Ligands for Modulating B-Cell ResponsesLAURA KIESSLING; Fiscal Year: 2006..We anticipate that our studies will provide insight into the signaling processes and may lead to new strategies for the generation of vaccines and/or compounds that inhibit autoimmune responses. ..
- MULTIVALENT PROTEIN CARBOHYDRATE INTERACTIONSLAURA KIESSLING; Fiscal Year: 2000..The tools and information gained from investigating multidentate interactions with the mannose-binding proteins will be applied to the study of the selectins. ..
- PROBES OF CARBOHYDRATE FUNCTIONLAURA KIESSLING; Fiscal Year: 2002..It is anticipated that these studies will lead to the development of new methodologies for the modulation of cell-cell and carbohydrate-receptor interactions. ..
- Multivalent Ligands as EffectorsLAURA KIESSLING; Fiscal Year: 2004..Our studies of leukocyte chemotaxis may lead to new strategies for the control of inflammation and a deeper understanding of the role of receptor clustering in GPCR-mediated signal amplification and integration. ..
- Glycopeptides and Other Non-Natural Variants: Probes of Carbohydrate FunctionLaura L Kiessling; Fiscal Year: 2011..This project is aimed at identifying DC-SIGN inhibitors and using compounds that bind to DC-SIGN to study its function. ..