Research Topics
| Kusum K KharbandaSummaryAffiliation: University of Nebraska Medical Center Country: USA Publications
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Detail Information
Publications
Role of elevated S-adenosylhomocysteine in rat hepatocyte apoptosis: protection by betaineKusum K Kharbanda
VA Alcohol Research Center, Department of Veterans Affairs Medical Center, Research Service 151, 4101 Woolworth Avenue, Omaha, NE 68105, USA
Biochem Pharmacol 70:1883-90. 2005..We conclude that ethanol-induced alteration in methionine metabolic pathways may play a crucial role in the pathologies associated with alcoholic liver injury and that betaine administration may have beneficial therapeutic effects...
Proteomics reveal a concerted upregulation of methionine metabolic pathway enzymes, and downregulation of carbonic anhydrase-III, in betaine supplemented ethanol-fed ratsKusum K Kharbanda
Department of Veterans Affairs Medical Centre, Omaha, NE 68105, USA
Biochem Biophys Res Commun 381:523-7. 2009..Carbonic anhydrase-III can function to resist oxidative stress, and we therefore hypothesise that carbonic anhydrase-III protein levels compromised by ethanol consumption, contribute to ethanol-induced redox stress...
Methionine metabolic pathway in alcoholic liver injuryKusum K Kharbanda
Research Service, Veterans Affairs Nebraska Western Iowa Healthcare System, Omaha, Nebraska 68105, USA
Curr Opin Clin Nutr Metab Care 16:89-95. 2013..To outline recent advances in the understanding of the consequences of the alterations in the methionine metabolic pathway and to present new treatment options for alcoholic liver disease (ALD)...- Impact of altered methylation in cytokine signaling and proteasome function in alcohol and viral-mediated diseasesKusum K Kharbanda
Research Service, Veterans Affairs Nebraska Western Iowa Health Care System, Omaha, NE 68105, USA
Alcohol Clin Exp Res 37:1-7. 2013..Two therapeutic modalities, betaine and S-adenosylmethionine, can correct methylation defects to attenuate many EtOH-induced liver changes, as well as improve IFN signaling pathways, thereby overcoming viral treatment resistance... - Alcoholic liver disease and methionine metabolismKusum K Kharbanda
Department of Veterans Affairs Medical Center, Research Service, Omaha, Nebraska 68105, USA
Semin Liver Dis 29:155-65. 2009..Thus, betaine is a promising therapeutic agent in relieving the methylation and other defects associated with alcoholic abuse... - Betaine administration corrects ethanol-induced defective VLDL secretionKusum K Kharbanda
Research Service 151, Department of Veterans Affairs Medical Center, 4101 Woolworth Avenue, Omaha, NE 68105, USA
Mol Cell Biochem 327:75-8. 2009..By increasing PEMT-mediated PC generation, betaine results in increased fat export from the liver and attenuates the development of alcoholic fatty liver... - Role of transmethylation reactions in alcoholic liver diseaseKusum K Kharbanda
Department of Veterans Affairs Medical Center, Research Service 151, 4101 Woolworth Avenue, Omaha, Nebraska 68105, USA
World J Gastroenterol 13:4947-54. 2007..In conclusion, betaine appears to be a promising therapeutic agent in relieving the methylation and other defects associated with alcoholic abuse... - Effect of malondialdehyde-acetaldehyde-protein adducts on the protein kinase C-dependent secretion of urokinase-type plasminogen activator in hepatic stellate cellsKusum K Kharbanda
Department of Veterans Affairs Medical Center, VA Alcohol Research Center, 4101 Woolworth Avenue, Omaha, NE 68105, USA
Biochem Pharmacol 63:553-62. 2002..These results indicate that MAA adducts via a PKC-mediated pathway may regulate plasmin-mediated matrix degradation in the liver, thereby contributing to the progression of hepatic fibrosis... - Accumulation of proteins bearing atypical isoaspartyl residues in livers of alcohol-fed rats is prevented by betaine administration: effects on protein-L-isoaspartyl methyltransferase activityKusum K Kharbanda
VA Alcohol Research Center, Department of Veterans Affairs Medical Center, Omaha, NE 68105, USA
J Hepatol 46:1119-25. 2007..We further sought to determine whether betaine administration could prevent the accumulation of these types of damaged proteins... - Betaine attenuates alcoholic steatosis by restoring phosphatidylcholine generation via the phosphatidylethanolamine methyltransferase pathwayKusum K Kharbanda
VA Alcohol Research Center, Department of Veterans Affairs Medical Center, Omaha, NE 68105, USA
J Hepatol 46:314-21. 2007..Therefore, we sought to determine whether the protective effect of betaine is via its effect on PEMT activity... - A comparison of the effects of betaine and S-adenosylmethionine on ethanol-induced changes in methionine metabolism and steatosis in rat hepatocytesKusum K Kharbanda
VA Alcohol Research Center, Department of Veterans Affairs Medical Center, Omaha, NE 68105, USA
J Nutr 135:519-24. 2005..Both SAM and betaine are effective in increasing the SAM:SAH ratio in hepatocytes and in attenuating hepatic steatosis; however, only betaine can effectively methylate homocysteine and prevent increased homocysteine release by the liver... - Transforming growth factor-beta induces contraction of activated hepatic stellate cellsKusum K Kharbanda
Department of Veterans Affairs Medical Center, VA Alcohol Research Center, 4101 Woolworth Avenue, Omaha, NE 68105, USA
J Hepatol 41:60-6. 2004..Recognizing the prominent roles that hepatic stellate cells (HSCs) and TGF-beta play in portal hypertension and fibrogenesis, respectively, we sought to evaluate the effect of TGF-beta on the contractility of activated HSCs... - Lysosomal leakage and lack of adaptation of hepatoprotective enzyme contribute to enhanced susceptibility to ethanol-induced liver injury in female ratsTerrence M Donohue
Liver Study Unit, VA Nebraska, Western Iowa Health Care Network, Omaha, Nebraska 68105, USA
Alcohol Clin Exp Res 31:1944-52. 2007..Here, we determined whether differential liver injury occurred in male and female rats after feeding these animals liquid diets containing either ethanol or isocaloric dextrose with fish oil as the sole source of lipid... - Impaired methylation as a novel mechanism for proteasome suppression in liver cellsNatalia A Osna
Liver Study Unit, The Omaha Veterans Affairs VA Medical Center, Omaha, NE 68105, USA
Biochem Biophys Res Commun 391:1291-6. 2010..We conclude that impaired methylation of proteasome subunits suppressed proteasome activity in liver cells indicating an additional, yet novel mechanism of proteasome activity regulation by ethanol... - Betaine lowers elevated s-adenosylhomocysteine levels in hepatocytes from ethanol-fed ratsAnthony J Barak
VA Alcohol Research Center, Department of Veterans Affairs Medical Center, Omaha, NE 68105, USA
J Nutr 133:2845-8. 2003.... - Malondialdehyde-acetaldehyde-adducted protein inhalation causes lung injuryTodd A Wyatt
Research Service, Veteran Affairs Nebraska Western Iowa Health Care System, Omaha, NE 68105, USA
Alcohol 46:51-9. 2012..These data further implicate MAA-adduct formation as a potential mechanism for smoke- and alcohol-induced lung injury... - Relationship between oxidative stress and hepatic glutathione levels in ethanol-mediated apoptosis of polarized hepatic cellsBenita L McVicker
Liver Study Unit, Department of Veterans Affairs Medical Center, Research Service 151, 4101 Woolworth Avenue, Omaha, NE 68105, USA
World J Gastroenterol 15:2609-16. 2009..To investigate the role of reactive oxygen species (ROS) in ethanol-mediated cell death of polarized hepatic (WIF-B) cells... - Hybrid malondialdehyde and acetaldehyde protein adducts form in the lungs of mice exposed to alcohol and cigarette smokeMichael L McCaskill
VA Research Service, Department of Veterans Affairs Medical Center, 985300 Nebraska Medical Center, Omaha, NE 68198, USA
Alcohol Clin Exp Res 35:1106-13. 2011..We hypothesized that chronic cigarette smoke and alcohol exposure in an in vivo mouse model would result in the in vivo formation of MAA adducts... - Ethanol feeding potentiates the pro-inflammatory response of Kupffer cells to cellular fibronectinRAZIA S AZIZ-SEIBLE
The Liver Study Unit, Department of Veterans Affairs Medical Center and the Departments of Internal Medicine and Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska 68105, USA
Alcohol Clin Exp Res 35:717-25. 2011..We propose that cFn directly stimulates Kupffer cells (KCs), which are involved in the early response to tissue damage, to produce factors that enhance the progression of alcohol-induced liver injury toward inflammation and fibrosis... - L-Buthionine (S,R) sulfoximine depletes hepatic glutathione but protects against ethanol-induced liver injuryTerrence M Donohue
Liver Study Unit, U S Department of Veterans Affairs Medical Center, University of Nebraska Medical Center, Omaha, Nebraska 68105, USA
Alcohol Clin Exp Res 31:1053-60. 2007..Here we investigated whether treatment with BSO enhanced ethanol-induced liver injury in mice... - Carbon tetrachloride-induced liver damage in asialoglycoprotein receptor-deficient miceShana R Dalton
Department of Internal Medicine, University of Nebraska Medical Center, Omaha, 68198 2000, USA
Biochem Pharmacol 77:1283-90. 2009..Thus, receptor-mediated endocytosis by the ASGP receptor could represent a novel molecular mechanism that is responsible for subsequent liver health or injury... - Ethanol blocks adenosine uptake via inhibiting the nucleoside transport system in bronchial epithelial cellsDiane S Allen-Gipson
Department of Internal Medicine, Pulmonary, Critical Care, Sleep and Allergy Section, 985815 Nebraska Medical Center, Omaha, NE 68198 5815, USA
Alcohol Clin Exp Res 33:791-8. 2009..Taken together, we hypothesized that ethanol impairs adenosine uptake via the nucleoside transport system... - Malondialdehyde-acetaldehyde adducts decrease bronchial epithelial wound repairTodd A Wyatt
Research Service, Department of Veterans Affairs Medical Center, 4101 Woolworth Avenue, Omaha, NE 68105, USA
Alcohol 36:31-40. 2005..Scavenger receptor-mediated activation of PKC alpha may function to reduce wound healing under conditions of alcohol and cigarette smoke exposure where malondialdehyde-acetaldehyde adducts may be present... - Effect of chronic ethanol administration on the in vitro production of proinflammatory cytokines by rat Kupffer cells in the presence of apoptotic cellsBenita L McVicker
Liver Study Unit, Department of Veterans Affairs Medical Center, and Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE 68105, USA
Alcohol Clin Exp Res 31:122-9. 2007.... - Ethanol and hepatitis C virus suppress peptide-MHC class I presentation in hepatocytes by altering proteasome functionNatalia A Osna
Research Service, Veterans Affairs Nebraska Western Iowa Health Care System, Omaha, Nebraska 68105, USA
Alcohol Clin Exp Res 36:2028-35. 2012..Here, we examined the effects of EtOH consumption by HCV core transgenic mice on proteasome activity in hepatocytic lysates and in partially purified 26S proteasome and the impact of these changes on antigen presentation... - Decreased proteasome activity is associated with increased severity of liver pathology and oxidative stress in experimental alcoholic liver diseaseTerrence M Donohue
Liver Study Unit, The Omaha Department of Veterans Affairs Medical Center, Omaha, Nebraska 68105, USA
Alcohol Clin Exp Res 28:1257-63. 2004..This study examined the relationship of proteasome activity and content with ethanol-induced oxidative stress and the degree of liver injury... - Betaine treatment attenuates chronic ethanol-induced hepatic steatosis and alterations to the mitochondrial respiratory chain proteomeKusum K Kharbanda
Research Service 151, Veterans Affairs Nebraska Western Iowa Health Care System, 4101 Woolworth Avenue, Omaha, NE 68105, USA
Int J Hepatol 2012:962183. 2012..Therefore, preservation of mitochondrial function may be another key molecular mechanism responsible for betaine hepatoprotection... - Inhibition of markers of hepatic stellate cell activation by the hormone relaxinRobert G Bennett
Department of Medical Research, Veterans Affairs Medical Center, 4101 Woolworth Ave, Omaha, NE 68105, USA
Biochem Pharmacol 66:867-74. 2003..Therefore, relaxin may be a useful approach in the treatment of hepatic fibrosis... - Inhibition of hepatic stellate cell collagen synthesis by N-(methylamino)isobutyric acidThomas L Freeman
Department of Internal Medicine, Section of Gastroenterology and Hepatology, University of Nebraska Medical Center, Omaha, NE 68105, USA
Biochem Pharmacol 63:697-706. 2002..MeAIB reduces the accumulation of collagen in CFSC-2G hepatic stellate cell cultures and in a CCl(4)-induced rat model of liver injury and fibrosis... - IL-10 receptor and coreceptor expression in quiescent and activated hepatic stellate cellsPhilippe Mathurin
Department of Pathology, Keck School of Medicine of the University of Southern California, Los Angeles 90089, USA
Am J Physiol Gastrointest Liver Physiol 282:G981-90. 2002..This shows concomitant induction of IL-10R but not CRF2-4 to that of IL-10 by activated HSC in vitro and in vivo and associated acquisition of the responsiveness to IL-10, entailing complex effects on HSC... - S-adenosylmethionine prevents chronic alcohol-induced mitochondrial dysfunction in the rat liverShannon M Bailey
Department of Environmental Health Sciences, School of Public Health, University of Alabama, Birmingham, AL 35294, USA
Am J Physiol Gastrointest Liver Physiol 291:G857-67. 2006..These findings reveal new molecular targets through which SAM may work to alleviate one critical component of alcohol-induced liver injury: mitochondria dysfunction... - Role of S-adenosylmethionine, folate, and betaine in the treatment of alcoholic liver disease: summary of a symposiumVishnudutt Purohit
Division of Metabolism and Health Effects, National Institute of Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD, USA
Am J Clin Nutr 86:14-24. 2007..Additionally, decreased concentrations of homocysteine can down-regulate endoplasmic reticulum stress, which leads to the attenuation of apoptosis and fatty acid synthesis...