Kusum K Kharbanda

Summary

Affiliation: University of Nebraska Medical Center
Country: USA

Publications

  1. ncbi request reprint Role of elevated S-adenosylhomocysteine in rat hepatocyte apoptosis: protection by betaine
    Kusum K Kharbanda
    VA Alcohol Research Center, Department of Veterans Affairs Medical Center, Research Service 151, 4101 Woolworth Avenue, Omaha, NE 68105, USA
    Biochem Pharmacol 70:1883-90. 2005
  2. pmc Proteomics reveal a concerted upregulation of methionine metabolic pathway enzymes, and downregulation of carbonic anhydrase-III, in betaine supplemented ethanol-fed rats
    Kusum K Kharbanda
    Department of Veterans Affairs Medical Centre, Omaha, NE 68105, USA
    Biochem Biophys Res Commun 381:523-7. 2009
  3. pmc Betaine treatment attenuates chronic ethanol-induced hepatic steatosis and alterations to the mitochondrial respiratory chain proteome
    Kusum K Kharbanda
    Research Service 151, Veterans Affairs Nebraska Western Iowa Health Care System, 4101 Woolworth Avenue, Omaha, NE 68105, USA
    Int J Hepatol 2012:962183. 2012
  4. doi request reprint Methionine metabolic pathway in alcoholic liver injury
    Kusum K Kharbanda
    Research Service, Veterans Affairs Nebraska Western Iowa Healthcare System, Omaha, Nebraska 68105, USA
    Curr Opin Clin Nutr Metab Care 16:89-95. 2013
  5. pmc Impact of altered methylation in cytokine signaling and proteasome function in alcohol and viral-mediated diseases
    Kusum K Kharbanda
    Research Service, Veterans Affairs Nebraska Western Iowa Health Care System, Omaha, NE 68105, USA
    Alcohol Clin Exp Res 37:1-7. 2013
  6. ncbi request reprint Effect of malondialdehyde-acetaldehyde-protein adducts on the protein kinase C-dependent secretion of urokinase-type plasminogen activator in hepatic stellate cells
    Kusum K Kharbanda
    Department of Veterans Affairs Medical Center, VA Alcohol Research Center, 4101 Woolworth Avenue, Omaha, NE 68105, USA
    Biochem Pharmacol 63:553-62. 2002
  7. ncbi request reprint Betaine attenuates alcoholic steatosis by restoring phosphatidylcholine generation via the phosphatidylethanolamine methyltransferase pathway
    Kusum K Kharbanda
    VA Alcohol Research Center, Department of Veterans Affairs Medical Center, Omaha, NE 68105, USA
    J Hepatol 46:314-21. 2007
  8. ncbi request reprint Role of transmethylation reactions in alcoholic liver disease
    Kusum K Kharbanda
    Department of Veterans Affairs Medical Center, Research Service 151, 4101 Woolworth Avenue, Omaha, Nebraska 68105, USA
    World J Gastroenterol 13:4947-54. 2007
  9. ncbi request reprint A comparison of the effects of betaine and S-adenosylmethionine on ethanol-induced changes in methionine metabolism and steatosis in rat hepatocytes
    Kusum K Kharbanda
    VA Alcohol Research Center, Department of Veterans Affairs Medical Center, Omaha, NE 68105, USA
    J Nutr 135:519-24. 2005
  10. doi request reprint Betaine administration corrects ethanol-induced defective VLDL secretion
    Kusum K Kharbanda
    Research Service 151, Department of Veterans Affairs Medical Center, 4101 Woolworth Avenue, Omaha, NE 68105, USA
    Mol Cell Biochem 327:75-8. 2009

Detail Information

Publications33

  1. ncbi request reprint Role of elevated S-adenosylhomocysteine in rat hepatocyte apoptosis: protection by betaine
    Kusum K Kharbanda
    VA Alcohol Research Center, Department of Veterans Affairs Medical Center, Research Service 151, 4101 Woolworth Avenue, Omaha, NE 68105, USA
    Biochem Pharmacol 70:1883-90. 2005
    ..We conclude that ethanol-induced alteration in methionine metabolic pathways may play a crucial role in the pathologies associated with alcoholic liver injury and that betaine administration may have beneficial therapeutic effects...
  2. pmc Proteomics reveal a concerted upregulation of methionine metabolic pathway enzymes, and downregulation of carbonic anhydrase-III, in betaine supplemented ethanol-fed rats
    Kusum K Kharbanda
    Department of Veterans Affairs Medical Centre, Omaha, NE 68105, USA
    Biochem Biophys Res Commun 381:523-7. 2009
    ..Carbonic anhydrase-III can function to resist oxidative stress, and we therefore hypothesise that carbonic anhydrase-III protein levels compromised by ethanol consumption, contribute to ethanol-induced redox stress...
  3. pmc Betaine treatment attenuates chronic ethanol-induced hepatic steatosis and alterations to the mitochondrial respiratory chain proteome
    Kusum K Kharbanda
    Research Service 151, Veterans Affairs Nebraska Western Iowa Health Care System, 4101 Woolworth Avenue, Omaha, NE 68105, USA
    Int J Hepatol 2012:962183. 2012
    ..Therefore, preservation of mitochondrial function may be another key molecular mechanism responsible for betaine hepatoprotection...
  4. doi request reprint Methionine metabolic pathway in alcoholic liver injury
    Kusum K Kharbanda
    Research Service, Veterans Affairs Nebraska Western Iowa Healthcare System, Omaha, Nebraska 68105, USA
    Curr Opin Clin Nutr Metab Care 16:89-95. 2013
    ..To outline recent advances in the understanding of the consequences of the alterations in the methionine metabolic pathway and to present new treatment options for alcoholic liver disease (ALD)...
  5. pmc Impact of altered methylation in cytokine signaling and proteasome function in alcohol and viral-mediated diseases
    Kusum K Kharbanda
    Research Service, Veterans Affairs Nebraska Western Iowa Health Care System, Omaha, NE 68105, USA
    Alcohol Clin Exp Res 37:1-7. 2013
    ..Two therapeutic modalities, betaine and S-adenosylmethionine, can correct methylation defects to attenuate many EtOH-induced liver changes, as well as improve IFN signaling pathways, thereby overcoming viral treatment resistance...
  6. ncbi request reprint Effect of malondialdehyde-acetaldehyde-protein adducts on the protein kinase C-dependent secretion of urokinase-type plasminogen activator in hepatic stellate cells
    Kusum K Kharbanda
    Department of Veterans Affairs Medical Center, VA Alcohol Research Center, 4101 Woolworth Avenue, Omaha, NE 68105, USA
    Biochem Pharmacol 63:553-62. 2002
    ..These results indicate that MAA adducts via a PKC-mediated pathway may regulate plasmin-mediated matrix degradation in the liver, thereby contributing to the progression of hepatic fibrosis...
  7. ncbi request reprint Betaine attenuates alcoholic steatosis by restoring phosphatidylcholine generation via the phosphatidylethanolamine methyltransferase pathway
    Kusum K Kharbanda
    VA Alcohol Research Center, Department of Veterans Affairs Medical Center, Omaha, NE 68105, USA
    J Hepatol 46:314-21. 2007
    ..Therefore, we sought to determine whether the protective effect of betaine is via its effect on PEMT activity...
  8. ncbi request reprint Role of transmethylation reactions in alcoholic liver disease
    Kusum K Kharbanda
    Department of Veterans Affairs Medical Center, Research Service 151, 4101 Woolworth Avenue, Omaha, Nebraska 68105, USA
    World J Gastroenterol 13:4947-54. 2007
    ..In conclusion, betaine appears to be a promising therapeutic agent in relieving the methylation and other defects associated with alcoholic abuse...
  9. ncbi request reprint A comparison of the effects of betaine and S-adenosylmethionine on ethanol-induced changes in methionine metabolism and steatosis in rat hepatocytes
    Kusum K Kharbanda
    VA Alcohol Research Center, Department of Veterans Affairs Medical Center, Omaha, NE 68105, USA
    J Nutr 135:519-24. 2005
    ..Both SAM and betaine are effective in increasing the SAM:SAH ratio in hepatocytes and in attenuating hepatic steatosis; however, only betaine can effectively methylate homocysteine and prevent increased homocysteine release by the liver...
  10. doi request reprint Betaine administration corrects ethanol-induced defective VLDL secretion
    Kusum K Kharbanda
    Research Service 151, Department of Veterans Affairs Medical Center, 4101 Woolworth Avenue, Omaha, NE 68105, USA
    Mol Cell Biochem 327:75-8. 2009
    ..By increasing PEMT-mediated PC generation, betaine results in increased fat export from the liver and attenuates the development of alcoholic fatty liver...
  11. ncbi request reprint Transforming growth factor-beta induces contraction of activated hepatic stellate cells
    Kusum K Kharbanda
    Department of Veterans Affairs Medical Center, VA Alcohol Research Center, 4101 Woolworth Avenue, Omaha, NE 68105, USA
    J Hepatol 41:60-6. 2004
    ..Recognizing the prominent roles that hepatic stellate cells (HSCs) and TGF-beta play in portal hypertension and fibrogenesis, respectively, we sought to evaluate the effect of TGF-beta on the contractility of activated HSCs...
  12. doi request reprint Alcoholic liver disease and methionine metabolism
    Kusum K Kharbanda
    Department of Veterans Affairs Medical Center, Research Service, Omaha, Nebraska 68105, USA
    Semin Liver Dis 29:155-65. 2009
    ..Thus, betaine is a promising therapeutic agent in relieving the methylation and other defects associated with alcoholic abuse...
  13. ncbi request reprint Accumulation of proteins bearing atypical isoaspartyl residues in livers of alcohol-fed rats is prevented by betaine administration: effects on protein-L-isoaspartyl methyltransferase activity
    Kusum K Kharbanda
    VA Alcohol Research Center, Department of Veterans Affairs Medical Center, Omaha, NE 68105, USA
    J Hepatol 46:1119-25. 2007
    ..We further sought to determine whether betaine administration could prevent the accumulation of these types of damaged proteins...
  14. ncbi request reprint Lysosomal leakage and lack of adaptation of hepatoprotective enzyme contribute to enhanced susceptibility to ethanol-induced liver injury in female rats
    Terrence M Donohue
    Liver Study Unit, VA Nebraska, Western Iowa Health Care Network, Omaha, Nebraska 68105, USA
    Alcohol Clin Exp Res 31:1944-52. 2007
    ..Here, we determined whether differential liver injury occurred in male and female rats after feeding these animals liquid diets containing either ethanol or isocaloric dextrose with fish oil as the sole source of lipid...
  15. pmc Impaired methylation as a novel mechanism for proteasome suppression in liver cells
    Natalia A Osna
    Liver Study Unit, The Omaha Veterans Affairs VA Medical Center, Omaha, NE 68105, USA
    Biochem Biophys Res Commun 391:1291-6. 2010
    ..We conclude that impaired methylation of proteasome subunits suppressed proteasome activity in liver cells indicating an additional, yet novel mechanism of proteasome activity regulation by ethanol...
  16. ncbi request reprint Betaine lowers elevated s-adenosylhomocysteine levels in hepatocytes from ethanol-fed rats
    Anthony J Barak
    VA Alcohol Research Center, Department of Veterans Affairs Medical Center, Omaha, NE 68105, USA
    J Nutr 133:2845-8. 2003
    ....
  17. pmc Ethanol and hepatitis C virus suppress peptide-MHC class I presentation in hepatocytes by altering proteasome function
    Natalia A Osna
    Research Service, Veterans Affairs Nebraska Western Iowa Health Care System, Omaha, Nebraska 68105, USA
    Alcohol Clin Exp Res 36:2028-35. 2012
    ..Here, we examined the effects of EtOH consumption by HCV core transgenic mice on proteasome activity in hepatocytic lysates and in partially purified 26S proteasome and the impact of these changes on antigen presentation...
  18. pmc Malondialdehyde-acetaldehyde-adducted protein inhalation causes lung injury
    Todd A Wyatt
    Research Service, Veteran Affairs Nebraska Western Iowa Health Care System, Omaha, NE 68105, USA
    Alcohol 46:51-9. 2012
    ..These data further implicate MAA-adduct formation as a potential mechanism for smoke- and alcohol-induced lung injury...
  19. pmc Relationship between oxidative stress and hepatic glutathione levels in ethanol-mediated apoptosis of polarized hepatic cells
    Benita L McVicker
    Liver Study Unit, Department of Veterans Affairs Medical Center, Research Service 151, 4101 Woolworth Avenue, Omaha, NE 68105, USA
    World J Gastroenterol 15:2609-16. 2009
    ..To investigate the role of reactive oxygen species (ROS) in ethanol-mediated cell death of polarized hepatic (WIF-B) cells...
  20. pmc Hybrid malondialdehyde and acetaldehyde protein adducts form in the lungs of mice exposed to alcohol and cigarette smoke
    Michael L McCaskill
    VA Research Service, Department of Veterans Affairs Medical Center, 985300 Nebraska Medical Center, Omaha, NE 68198, USA
    Alcohol Clin Exp Res 35:1106-13. 2011
    ..We hypothesized that chronic cigarette smoke and alcohol exposure in an in vivo mouse model would result in the in vivo formation of MAA adducts...
  21. doi request reprint Ethanol feeding potentiates the pro-inflammatory response of Kupffer cells to cellular fibronectin
    RAZIA S AZIZ-SEIBLE
    The Liver Study Unit, Department of Veterans Affairs Medical Center and the Departments of Internal Medicine and Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska 68105, USA
    Alcohol Clin Exp Res 35:717-25. 2011
    ..We propose that cFn directly stimulates Kupffer cells (KCs), which are involved in the early response to tissue damage, to produce factors that enhance the progression of alcohol-induced liver injury toward inflammation and fibrosis...
  22. ncbi request reprint Effect of chronic ethanol administration on the in vitro production of proinflammatory cytokines by rat Kupffer cells in the presence of apoptotic cells
    Benita L McVicker
    Liver Study Unit, Department of Veterans Affairs Medical Center, and Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE 68105, USA
    Alcohol Clin Exp Res 31:122-9. 2007
    ....
  23. ncbi request reprint L-Buthionine (S,R) sulfoximine depletes hepatic glutathione but protects against ethanol-induced liver injury
    Terrence M Donohue
    Liver Study Unit, U S Department of Veterans Affairs Medical Center, University of Nebraska Medical Center, Omaha, Nebraska 68105, USA
    Alcohol Clin Exp Res 31:1053-60. 2007
    ..Here we investigated whether treatment with BSO enhanced ethanol-induced liver injury in mice...
  24. pmc Ethanol blocks adenosine uptake via inhibiting the nucleoside transport system in bronchial epithelial cells
    Diane S Allen-Gipson
    Department of Internal Medicine, Pulmonary, Critical Care, Sleep and Allergy Section, 985815 Nebraska Medical Center, Omaha, NE 68198 5815, USA
    Alcohol Clin Exp Res 33:791-8. 2009
    ..Taken together, we hypothesized that ethanol impairs adenosine uptake via the nucleoside transport system...
  25. pmc Carbon tetrachloride-induced liver damage in asialoglycoprotein receptor-deficient mice
    Shana R Dalton
    Department of Internal Medicine, University of Nebraska Medical Center, Omaha, 68198 2000, USA
    Biochem Pharmacol 77:1283-90. 2009
    ..Thus, receptor-mediated endocytosis by the ASGP receptor could represent a novel molecular mechanism that is responsible for subsequent liver health or injury...
  26. ncbi request reprint Decreased proteasome activity is associated with increased severity of liver pathology and oxidative stress in experimental alcoholic liver disease
    Terrence M Donohue
    Liver Study Unit, The Omaha Department of Veterans Affairs Medical Center, Omaha, Nebraska 68105, USA
    Alcohol Clin Exp Res 28:1257-63. 2004
    ..This study examined the relationship of proteasome activity and content with ethanol-induced oxidative stress and the degree of liver injury...
  27. ncbi request reprint Malondialdehyde-acetaldehyde adducts decrease bronchial epithelial wound repair
    Todd A Wyatt
    Research Service, Department of Veterans Affairs Medical Center, 4101 Woolworth Avenue, Omaha, NE 68105, USA
    Alcohol 36:31-40. 2005
    ..Scavenger receptor-mediated activation of PKC alpha may function to reduce wound healing under conditions of alcohol and cigarette smoke exposure where malondialdehyde-acetaldehyde adducts may be present...
  28. ncbi request reprint Inhibition of markers of hepatic stellate cell activation by the hormone relaxin
    Robert G Bennett
    Department of Medical Research, Veterans Affairs Medical Center, 4101 Woolworth Ave, Omaha, NE 68105, USA
    Biochem Pharmacol 66:867-74. 2003
    ..Therefore, relaxin may be a useful approach in the treatment of hepatic fibrosis...
  29. doi request reprint Alcohol consumption decreases rat hepatic creatine biosynthesis via altered guanidinoacetate methyltransferase activity
    Kusum K Kharbanda
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska Research Service, Veterans Affairs Nebraska Western Iowa Health Care System, Omaha, Nebraska Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska
    Alcohol Clin Exp Res 38:641-8. 2014
    ..As GAMT is the major utilizer of SAM, the purpose of the study was to examine the effects of ethanol (EtOH) on liver creatine levels and GAMT activity...
  30. ncbi request reprint Inhibition of hepatic stellate cell collagen synthesis by N-(methylamino)isobutyric acid
    Thomas L Freeman
    Department of Internal Medicine, Section of Gastroenterology and Hepatology, University of Nebraska Medical Center, Omaha, NE 68105, USA
    Biochem Pharmacol 63:697-706. 2002
    ..MeAIB reduces the accumulation of collagen in CFSC-2G hepatic stellate cell cultures and in a CCl(4)-induced rat model of liver injury and fibrosis...
  31. ncbi request reprint S-adenosylmethionine prevents chronic alcohol-induced mitochondrial dysfunction in the rat liver
    Shannon M Bailey
    Department of Environmental Health Sciences, School of Public Health, University of Alabama, Birmingham, AL 35294, USA
    Am J Physiol Gastrointest Liver Physiol 291:G857-67. 2006
    ..These findings reveal new molecular targets through which SAM may work to alleviate one critical component of alcohol-induced liver injury: mitochondria dysfunction...
  32. ncbi request reprint IL-10 receptor and coreceptor expression in quiescent and activated hepatic stellate cells
    Philippe Mathurin
    Department of Pathology, Keck School of Medicine of the University of Southern California, Los Angeles 90089, USA
    Am J Physiol Gastrointest Liver Physiol 282:G981-90. 2002
    ..This shows concomitant induction of IL-10R but not CRF2-4 to that of IL-10 by activated HSC in vitro and in vivo and associated acquisition of the responsiveness to IL-10, entailing complex effects on HSC...
  33. ncbi request reprint Role of S-adenosylmethionine, folate, and betaine in the treatment of alcoholic liver disease: summary of a symposium
    Vishnudutt Purohit
    Division of Metabolism and Health Effects, National Institute of Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD, USA
    Am J Clin Nutr 86:14-24. 2007
    ..Additionally, decreased concentrations of homocysteine can down-regulate endoplasmic reticulum stress, which leads to the attenuation of apoptosis and fatty acid synthesis...