Research Topics
| Ashwani KhannaSummaryAffiliation: University of Maryland Country: USA Publications
Research Grants
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Detail Information
Publications
Enhanced susceptibility of cyclin kinase inhibitor p21 knockout mice to high fat diet induced atherosclerosisAshwani K Khanna
Department of Medicine Cardiology, University of Maryland, Baltimore, USA
J Biomed Sci 16:66. 2009..This opens the possibility of p21 protein as a therapeutic tool to control progression of atherosclerosis...- Targeted in vitro and in vivo gene transfer into T lymphocytes: potential of direct inhibition of allo-immune activationAshwani K Khanna
Department of Medicine, Division of Cardiology, University of Maryland, Baltimore, MD 21201, USA
BMC Immunol 7:26. 2006..Lymphocyte proliferation was quantified by 3H-thymidine uptake assay; IL-2 mRNA expression was studied by RT-PCR and using Real Time PCR assay, we monitored the CD3, p21, TNF-alpha and IFN-gamma mRNA expression... 
NADPH oxidase subunits (NOX-1, p22phox, Rac-1) and tacrolimus-induced nephrotoxicity in a rat renal transplant modelAshwani K Khanna
Division of Cardiology, University of Maryland, 22 S Greene Street S 3B08, Baltimore, MD 21201, USA
Nephrol Dial Transplant 22:376-85. 2007..We examined whether anti-TGF-beta antibody limits nephrotoxicity produced by tacrolimus (TAC) and whether this altered genes that regulate oxidative stress...- Expression of TGF-beta and fibrogenic genes in transplant recipients with tacrolimus and cyclosporine nephrotoxicityAshwani Khanna
Department of Medicine, Division of Nephrology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
Kidney Int 62:2257-63. 2002.... - Wideband high-frequency echocardiography to evaluate myocardial infarct sizeJianping Xu
Division of Cardiology, University of Maryland School of Medicine, 655 W Baltimore St, Baltimore, MD 21201, USA
J Ultrasound Med 28:1527-34. 2009..This study was designed to validate the feasibility of wideband high-frequency ultrasound imaging to resolve in vivo the degree, location, and morphologic changes of myocardial infarction (MI) in a rat model... - Reciprocal role of cyclins and cyclin kinase inhibitor p21WAF1/CIP1 on lymphocyte proliferation, allo-immune activation and inflammationAshwani K Khanna
Department of Medicine Nephrology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
BMC Immunol 6:22. 2005..We hypothesize that the cell cycle control and inflammation are parallel events, inhibition of cellular proliferation by cyclin kinase inhibitor specifically p21 will limit inflammation and prevent allograft rejection... - Post-translational modification of manganese superoxide dismutase in acutely rejecting cardiac transplants: role of inducible nitric oxide synthaseVani Nilakantan
Division of Transplant Surgery, Department of Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
J Heart Lung Transplant 24:1591-9. 2005..In this study, we examined the effect of inducible nitric oxide synthase (iNOS) on MnSOD expression, activity and nitration in acutely rejecting cardiac transplants... - Antagonizing reactive oxygen by treatment with a manganese (III) metalloporphyrin-based superoxide dismutase mimetic in cardiac transplantsVani Nilakantan
Department of Surgery, Division of Transplant Surgery, Medical College of Wisconsin, Milwaukee, Wis 53226, USA
J Thorac Cardiovasc Surg 131:898-906. 2006..CONCLUSIONS: The mechanism of the beneficial effect of MnTmPyP on graft function appear related, in part, to scavenging O2*- and by decreasing apoptotic signaling rather than an effect on inflammatory cytokine gene expression... - Beneficial effect of alpha-tocopheryl succinate in rat cardiac transplantsThanh K Nguyen
Department of Surgery, Division of Transplant Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
J Heart Lung Transplant 25:707-15. 2006..This might explain the equipotent action of alpha-TPGS vs low-dose CsA and its action to potentiate graft survival and limit graft rejection and dysfunction... - Nitric oxide formation in acutely rejecting cardiac allografts correlates with GTP cyclohydrolase I activityGalen M Pieper
Division of Transplant Surgery, Department of Surgery, Medical College of Wisconsin, Milwaukee, WI, USA
Biochem J 391:541-7. 2005..These findings provide insight into a potential pathway regulating NO bioactivity in graft rejection. Such knowledge may potentially assist in the design of newer strategies to prevent acute graft rejection... - Recombinant p21 protein inhibits lymphocyte proliferation and transcription factorsAshwani K Khanna
Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53226, USA
J Immunol 174:7610-7. 2005.... - Protective mechanisms of a metalloporphyrinic peroxynitrite decomposition catalyst, WW85, in rat cardiac transplantsGalen M Pieper
Division of Transplant Surgery, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, Wisconsin 53226, USA
J Pharmacol Exp Ther 314:53-60. 2005..Rather, effects seem to be downstream on decreased protein nitration, decreased lipid peroxidation, and decreased PARP activation... - Anti-transforming growth factor antibody at low but not high doses limits cyclosporine-mediated nephrotoxicity without altering rat cardiac allograft survival: potential of therapeutic applicationsAshwani K Khanna
Division of Nephrology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
Circulation 110:3822-9. 2004..Transforming growth factor-beta (TGF-beta) is one of the most potent mediators of the fibrogenic effects of cyclosporine... - Treatment with {alpha}-phenyl-N-tert-butylnitrone, a free radical-trapping agent, abrogates inflammatory cytokine gene expression during alloimmune activation in rat cardiac allograftsGalen M Pieper
Division of Transplant Surgery, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, WI 53226, USA
J Pharmacol Exp Ther 312:774-9. 2005..Thus, PBN retains significant anti-inflammatory properties through its action to down-regulate cytokine gene expression that contribute to protection against acute alloimmune activation in cardiac allografts... - Mechanisms of the protective action of diethyldithiocarbamate-iron complex on acute cardiac allograft rejectionGalen M Pieper
Division of Transplant Surgery, Medical College of Wisconsin, 9200 W Wisconsin Avenue, Milwaukee, WI 53226, USA
Am J Physiol Heart Circ Physiol 284:H1542-51. 2003..Gene expression for the proinflammatory cytokine interferon-gamma was also decreased by DETC-Fe. Thus DETC-Fe limits NF-kappaB-dependent gene expression and possesses significant immunosuppressive properties... - The immunosuppressive agent tacrolimus induces p21WAF/CIP1WAF1/CIP1 via TGF-beta secretionAshwani K Khanna
Medical College of Wisconsin, 8701, Watertown Plank Road, Milwaukee, WI 53226, USA
Biochem Biophys Res Commun 303:266-72. 2003..Strategies involving p21WAF/CIP1 induction should be considered a viable alternative strategy to achieve immunosuppression possibly with reduced toxicity associated with current immunosuppression... - Analysis of transforming growth factor-beta and profibrogenic molecules in a rat cardiac allograft model treated with cyclosporineAshwani K Khanna
Department of Medicine (Nephrology, Medical College of Wisconsin, Milwaukee, Wisconsin 53215, USA
Transplantation 73:1543-9. 2002..Therapies designed to inhibit expression of TGF-beta could ameliorate CsA-associated nephrotoxicity in cardiac transplant recipients... - TGF-beta1, FGF-2, and receptor mRNA expression in suture mesenchyme and dura versus underlying brain in fusing and nonfusing mouse cranial suturesArun K Gosain
Department of Plastic and Reconstructive Surgery, Medical College of Wisconsin, 9200 W Wisconsin Avenue, Milwaukee, WI 53226, USA
Plast Reconstr Surg 113:1675-84. 2004..Understanding these differences may eventually help to identify possible targets and time windows by which to most effectively modulate cranial suture fusion... - Inhibition of nitrosylation, nitration, lymphocyte proliferation, and gene expression in acute and delayed cardiac allograft rejection by an orally active dithiocarbamateGalen M Pieper
Department of Surgery Division of Transplant Surgery, Medical College of Wisconsin, Milwaukee, WI 53226, USA
J Cardiovasc Pharmacol 43:522-30. 2004..These studies suggest that NOX-700 is protective in cardiac rejection, in part, by scavenging of NO and by limiting lymphocyte activation infiltration... - Variable efficacy of N6-(1-iminoethyl)-L-lysine in acute cardiac transplant rejectionGalen M Pieper
Division of Transplant Surgery, Department of Surgery, Cardiovascular Center, Medical College of Wisconsin, Milwaukee 53226, USA
Am J Physiol Heart Circ Physiol 286:H525-34. 2004..These studies have important implications in understanding the dual role of NO in acute rejection and help to reconcile discrepancies in the literature...
Research Grants
- TGF-BETA AND IMMUNOSUPPRESSION IN ORGAN TRANSPLANTATIONAshwani Khanna; Fiscal Year: 2002..These studies will directly determine the role (if any) of the TGF-B and/or p21 pathways in the efficacy, and TGF-B in the toxicity, induced by CsA and tacrolimus. ..