Affiliation: University of North Carolina
- Identification of salivary mucin MUC7 binding proteins from Streptococcus gordoniiMehmet Kesimer
Department of Biochemistry, Faculty of Medicine, University of Gazi, 06510 Besevler, Ankara, Turkey
BMC Microbiol 9:163. 2009..Identification and characterization of the specific interacting proteins on the bacterial cell surface, termed adhesins, are crucial to further understand host-pathogen interactions...
- Mass spectrometric analysis of mucin core proteinsMehmet Kesimer
Department of Biochemistry and Biophysics Cystic Fibrosis Pulmonary Research Center, University of North Carolina, 4021 Thurston Bowles Bldg CB 7248, Chapel Hill, NC, USA
Methods Mol Biol 842:67-79. 2012..In this chapter, we present our current protocol employing a modified shotgun proteomic approach to identify these complex glycoproteins...
- Unpacking a gel-forming mucin: a view of MUC5B organization after granular releaseMehmet Kesimer
1Department of Biochemistry and Biophysics and Cystic Fibrosis Pulmonary Research and Treatment Center, Chapel Hill, NC, USA
Am J Physiol Lung Cell Mol Physiol 298:L15-22. 2010....
- Analyzing the functions of large glycoconjugates through the dissipative properties of their absorbed layers using the gel-forming mucin MUC5B as an exampleMehmet Kesimer
Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599, USA
Glycobiology 18:463-72. 2008..g. binding viruses, but could also be informative to the surfaces (often ciliated) onto which such mucus films are attached...
- Tracheobronchial air-liquid interface cell culture: a model for innate mucosal defense of the upper airways?Mehmet Kesimer
Dept of Biochemistry and Biophysics, Univ of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Am J Physiol Lung Cell Mol Physiol 296:L92-L100. 2009..This shows that the primary cell culture system is an important model for study of aspects of innate defense of the upper airways related specifically to mucus consisting solely of airway cell products...
- Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defenseMehmet Kesimer
Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
FASEB J 23:1858-68. 2009..Taken together, these findings suggest that airway epithelial cells release exosome-like vesicles and that these structures may be involved in diverse physiological processes in airway biology, including innate mucosal defense...
- A periciliary brush promotes the lung health by separating the mucus layer from airway epitheliaBrian Button
Cystic Fibrosis Research and Treatment Center, University of North Carolina, Chapel Hill, NC 27599 7248, USA
Science 337:937-41. 2012..The relative osmotic moduli of the mucus and periciliary brush layers explain both the stability of mucus clearance in health and its failure in airway disease...
- Identification of SPLUNC1's ENaC-inhibitory domain yields novel strategies to treat sodium hyperabsorption in cystic fibrosis airwaysCarey A Hobbs
Cystic Fibrosis Pulmonary Research and Treatment Center, Department of Prosthodontics, University of North Carolina, Chapel Hill, 7125 Thurston Bowles Bldg, Chapel Hill, NC 27599 7248, USA
FASEB J 26:4348-59. 2012..Furthermore, the robust inhibition of ENaC by the G22-A39 peptide suggests that this peptide may be suitable for treating CF lung disease...
- Mapping the protein domain structures of the respiratory mucins: a mucin proteome coverage studyRui Cao
Cystic Fibrosis and Pulmonary Research Center, Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, North Carolina, United States
J Proteome Res 11:4013-23. 2012....
- The cystic fibrosis transmembrane conductance regulator is regulated by a direct interaction with the protein phosphatase 2AWilliam R Thelin
Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
J Biol Chem 280:41512-20. 2005..Furthermore, PP2A may be a clinically relevant drug target for CF, which should be considered in future studies...
- Studying mucin secretion from human bronchial epithelial cell primary culturesLubna H Abdullah
Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, NC, USA
Methods Mol Biol 842:259-77. 2012..These results indicate that the more nonspecific assays are suitable to assess overall secretory responses by goblet cells, but are likely unsuited for specific measurements of polymeric mucins, per se...
- Innate immunity and mucus structure and functionJohn K Sheehan
Cystic Fibrosis Center, Campus Box 7248, 4019a Thurston Bowles, University of North Carolina at Chapel Hill, NC 27599, USA
Novartis Found Symp 279:155-66; discussion 167-9, 216-9. 2006....
- Reduced three-dimensional motility in dehydrated airway mucus prevents neutrophil capture and killing bacteria on airway epithelial surfacesHirotoshi Matsui
Cystic Fibrosis Pulmonary Research and Treatment Center and Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599, USA
J Immunol 175:1090-9. 2005..We conclude that concentrated ("thick") mucus inhibits neutrophil migration and killing and is a key component in the failure of defense against chronic airways infection in CF...
- Hemoglobin Raleigh results in factitiously low hemoglobin A1c when evaluated via immunoassay analyzerNina Jain
Division of Pediatric Endocrinology, Department of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
J Diabetes Complications 25:14-8. 2011..Glycosylated hemoglobin (HbA1c) is commonly used to assess long-term blood glucose control in patients with diabetes mellitus. Numerous conditions including hemoglobinopathies can alter HbA1c measurements and cause misleading results...
- Chronic exposure to beta-blockers attenuates inflammation and mucin content in a murine asthma modelLong P Nguyen
Department of Pharmacological and Pharmaceutical Sciences, University of Houston, College of Pharmacy, 4800 Calhoun, Houston, TX 77204 5037, USA
Am J Respir Cell Mol Biol 38:256-62. 2008..Similar to heart failure, our results provide a second disease model in which beta-blockers producing an acutely detrimental effect may provide a therapeutically beneficial effect with chronic administration...