Research Topics
Species | T K KerppolaSummaryAffiliation: University of Michigan Country: USA Publications
Research Grants
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Detail Information
Publications
Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cellsTom K Kerppola
Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
Nat Protoc 1:1278-86. 2006..It is technically straightforward and can be performed using a regular fluorescence microscope and standard molecular biology and cell culture reagents...- Fos and Jun bend the AP-1 site: effects of probe geometry on the detection of protein-induced DNA bendingT K Kerppola
Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor 48109 0650, USA
Proc Natl Acad Sci U S A 93:10117-22. 1996..The analogous phase- and shape-dependence of the electrophoretic mobilities of the Fos-Jun-AP-1 complex and an intrinsic DNA bend confirm that Fos and Jun bend DNA, which may contribute to their functions in transcription regulation... - Overcoming uncertainty through advances in fluorescence imaging of molecular processes in cellsTom K Kerppola
Department of Biological Chemistry, Howard Hughes Medical Institute, University of Michigan School of Medicine, Ann Arbor 48109-0650, USA
Methods 45:183-4. 2008 - Changes in the distributions and dynamics of polycomb repressive complexes during embryonic stem cell differentiationXiaojun Ren
Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109 0650
Mol Cell Biol 28:2884-95. 2008..Epigenetic reprogramming during ES cell differentiation is therefore associated with global changes in the subnuclear distributions and dynamics of CBX protein complexes... - Visualization of molecular interactions by fluorescence complementationTom K Kerppola
Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109 0650, USA
Nat Rev Mol Cell Biol 7:449-56. 2006..Modified forms of this assay have been used to visualize the competition between alternative interaction partners and the covalent modification of proteins by ubiquitin-family peptides... - Bimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cellsTom K Kerppola
Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109 0650, USA
Annu Rev Biophys 37:465-87. 2008..The BiFC assay is technically straightforward and can be performed using standard molecular biology and cell culture reagents and a regular fluorescence microscope or flow cytometer... - Visualization of molecular interactions using bimolecular fluorescence complementation analysis: characteristics of protein fragment complementationTom K Kerppola
Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109 0650, USA
Chem Soc Rev 38:2876-86. 2009..The review will be of interest to scientists interested in the investigation of macromolecular interactions or modifications who need exquisite sensitivity for the detection of their complexes or conjugates of interest... - Polycomb group complexes--many combinations, many functionsTom K Kerppola
Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109 0650, USA
Trends Cell Biol 19:692-704. 2009..Future studies of this enigmatic group of developmental regulators are certain to produce unanticipated discoveries... - The transcription activation domains of Fos and Jun induce DNA bending through electrostatic interactionsT K Kerppola
Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor 48109 0650, USA
EMBO J 16:2907-16. 1997..Consequently, regions outside the minimal DNA-binding domain can influence DNA structure, and may thereby contribute to the architectural reorganization of the promoter region required for gene activation... 
Transcriptional cooperativity: bending over backwards and doing the flipT K Kerppola
Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor 48109 0650, USA
Structure 6:549-54. 1998..Studies of the interaction between Fos-Jun and NFAT1 in solution corroborate the crystallographic analysis. These results manifest the flexibility required for cooperative binding to composite regulatory elements...- Comparison of DNA bending by Fos-Jun and phased A tracts by multifactorial phasing analysisT K Kerppola
Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109 0650, USA
Biochemistry 36:10872-84. 1997..This reversal of the phase dependence of the electrophoretic mobility variation was also observed for complexes formed by truncated Fos and Jun. Thus, the phase-dependent mobility variation of Fos and Jun complexes is due to DNA bending... - Dynamics of Fos-Jun-NFAT1 complexesV R Ramirez-Carrozzi
Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109-0650, USA
Proc Natl Acad Sci U S A 98:4893-8. 2001..Thus, the orientation of heterodimer binding can influence both the dynamics and promoter selectivity of multiprotein transcription regulatory complexes... - Long-range electrostatic interactions influence the orientation of Fos-Jun binding at AP-1 sitesV R Ramirez-Carrozzi
Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109-0650, USA
J Mol Biol 305:411-27. 2001..Consequently, long-range electrostatic interactions influence the architecture of nucleoprotein complexes... - Control of the orientation of Fos-Jun binding and the transcriptional cooperativity of Fos-Jun-NFAT1 complexesV R Ramirez-Carrozzi
Howard Hughes Medical InstituteM Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109-0650, USA
J Biol Chem 276:21797-808. 2001..Consequently, the orientation of Fos-Jun binding can influence transcriptional activity by altering cooperative interactions with other transcription regulatory proteins... - Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementationChang Deng Hu
Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USA
Mol Cell 9:789-98. 2002..These results attest to the general applicability of the BiFC assay for studies of protein interactions... - Close encounters of many kinds: Fos-Jun interactions that mediate transcription regulatory specificityY Chinenov
Howard Hughes Medical Institute, University of Michigan Medical School Ann Arbor, Michigan, MI 48109-0650, USA
Oncogene 20:2438-52. 2001..The gene-specific architecture of these complexes can mediate the selective control of transcriptional activity... - Visualization of protein interactions in living cells using bimolecular fluorescence complementation (BiFC) analysisChang-Deng Hu
Howard Hughes Medical Institute and University of Michigan Medical School, Ann Arbor, Michigan, USA
Curr Protoc Cell Biol . 2006..This enables comparison of subcellular distributions of different protein complexes in the same cell and allows analysis of competition between mutually exclusive interaction partners... - Different polycomb group CBX family proteins associate with distinct regions of chromatin using nonhomologous protein sequencesClaudius Vincenz
Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109 0650, USA
Proc Natl Acad Sci U S A 105:16572-7. 2008..We conclude that different CBX proteins are recruited to distinct chromatin regions through nonconserved interactions, expanding the regulatory diversity of polycomb group proteins... - Bimolecular fluorescence complementation: visualization of molecular interactions in living cellsTom K Kerppola
Department of Biological Chemistry, Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
Methods Cell Biol 85:431-70. 2008..These fluorescence complementation assays have a great potential to illuminate a variety of biological interactions in the future... - Bimolecular fluorescence complementation analysis of inducible protein interactions: effects of factors affecting protein folding on fluorescent protein fragment associationAaron M Robida
Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109 0650, USA
J Mol Biol 394:391-409. 2009..Conditional BiFC complex formation depends on the folding efficiencies of fluorescent protein fragments and can be affected by the cellular protein folding environment... - Ubiquitin-mediated fluorescence complementation reveals that Jun ubiquitinated by Itch/AIP4 is localized to lysosomesDeyu Fang
Howard Hughes Medical Institute, Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109 0650, USA
Proc Natl Acad Sci U S A 101:14782-7. 2004..The visualization of ubiquitinated Jun in living cells has uncovered a lysosomal pathway for Jun degradation that involves ubiquitination by Itch/AIP4... - Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysisChang-Deng Hu
Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109-0650, USA
Nat Biotechnol 21:539-45. 2003..Multicolor BiFC enables visualization of interactions between different proteins in the same cell and comparison of the efficiencies of complex formation with alternative interaction partners... - Visualization of protein interactions in living cells using bimolecular fluorescence complementation (BiFC) analysisChang-Deng Hu
Howard Hughes Medical Institute and University of Michigan Medical School, Ann Arbor, Michigan, USA
Curr Protoc Protein Sci . 2005..This enables comparison of subcellular distributions of different protein complexes in the same cell and allows analysis of competition between mutually exclusive interaction partners... - Lysosomal localization of ubiquitinated Jun requires multiple determinants in a lysine-27-linked polyubiquitin conjugateHiromi Ikeda
Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109 0650, USA
Mol Biol Cell 19:4588-601. 2008..Lysosomal localization of the conjugate requires determinants in Jun and in ubiquitin that are recognized in part by TSG101 and HRS, facilitating selective translocation and degradation of ubiquitinated Jun... - Opposing roles of FoxP1 and Nfat3 in transcriptional control of cardiomyocyte hypertrophyShoumei Bai
Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109 0650, USA
Mol Cell Biol 31:3068-80. 2011..FoxP1 counteracted hypertrophic cardiomyocyte growth, and connexin 43 mislocalization caused by cnNfat3 expression. These data suggest that the opposing transcriptional activities of FoxP1 and Nfat3 maintain cardiomyocyte homeostasis... - REST interacts with Cbx proteins and regulates polycomb repressive complex 1 occupancy at RE1 elementsXiaojun Ren
Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109 0650, USA
Mol Cell Biol 31:2100-10. 2011..The opposite effects of REST on PRC1 occupancy at different RE1 elements contributed to the gene-specific control of PRC1 functions during ES cell differentiation... - Synergistic transcription activation by Maf and Sox and their subnuclear localization are disrupted by a mutation in Maf that causes cataractNirmala Rajaram
Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109-0650, USA
Mol Cell Biol 24:5694-709. 2004..The mislocalization of normal cellular proteins to these foci provides a potential explanation for the dominant disease phenotype of the R288P mutation in Maf... - Visualization of Myc/Max/Mad family dimers and the competition for dimerization in living cellsAsya V Grinberg
Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109 0650, USA
Mol Cell Biol 24:4294-308. 2004..The distinct subcellular locations and the differences between the efficiencies of dimerization with Max indicate that Mad3 and Mad4 are likely to modulate transcription activation by Myc at least in part through distinct mechanisms... 
Visualization of protein interactions in living Caenorhabditis elegans using bimolecular fluorescence complementation analysisY John Shyu
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907 2091, USA
Nat Protoc 3:588-96. 2008..When expression of BiFC fusion proteins is induced by heat shock, the fluorescent signals can be visualized as early as 30 min after induction and last for 24 h in transgenic animals. The entire procedure takes 2-3 weeks to complete...- The F-box protein Skp2 participates in c-Myc proteosomal degradation and acts as a cofactor for c-Myc-regulated transcriptionNatalie von der Lehr
Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, 750 07 Uppsala, Sweden
Mol Cell 11:1189-200. 2003..The results suggest that Skp2 is a transcriptional cofactor for c-Myc and indicates a close relationship between transcription activation and transcription factor ubiquitination... - Probing FinO-FinP RNA interactions by site-directed protein-RNA crosslinking and gelFRETAlexandru F Ghetu
Department of Biochemistry, University of Alberta, Edmonton, Canada
RNA 8:816-23. 2002..These data suggest that significant conformational adjustments in the protein and/or the RNA accompany complex formation... - Complementary methods for studies of protein interactions in living cellsTom K Kerppola
Nat Methods 3:969-71. 2006
Research Grants
- Optimization of Bimolecular Fluorescence Complementation Probes for New Imaging FTom Kerppola; Fiscal Year: 2009..This work will improve our understanding of interactions among cellular components that are important for the health of the cell and the organism. ..
- Optimization of Bimolecular Fluorescence Complementation Probes for New Imaging FTzvi Tzfira; Fiscal Year: 2010..This work will improve our understanding of interactions among cellular components that are important for the health of the cell and the organism. ..
- Optimization of Bimolecular Fluorescence Complementation Probes for New Imaging FTom Kerppola; Fiscal Year: 2009..This work will improve our understanding of interactions among cellular components that are important for the health of the cell and the organism. ..
- Visualization of Combinatorial Epigenetic Marks and Complexes in Animals Using FlTom Kerppola; Fiscal Year: 2010..In this project, we develop molecules that enable researchers to see the combinations of tags in individual cells in living animals. ..