George L Kenyon

Summary

Affiliation: University of Michigan
Country: USA

Publications

  1. ncbi request reprint How I became a biochemist
    George L Kenyon
    College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109, USA
    IUBMB Life 57:375-8. 2005
  2. ncbi request reprint Defining the mandate of proteomics in the post-genomics era: workshop report
    George L Kenyon
    College of Pharmacy, University of Michigan, Ann Arbor, MI 48109 1065, USA
    Mol Cell Proteomics 1:763-80. 2002
  3. doi request reprint Using directed evolution to probe the substrate specificity of mandelamide hydrolase
    Pan Fen Wang
    College of Pharmacy, University of Michigan, 428 Church St, Ann Arbor, MI 48109, USA
    Protein Eng Des Sel 22:103-10. 2009
  4. ncbi request reprint Relating structure to mechanism in creatine kinase
    Michael J McLeish
    College of Pharmacy, University of Michigan, Ann Arbor, MI, USA
    Crit Rev Biochem Mol Biol 40:1-20. 2005
  5. pmc Identification and characterization of a mandelamide hydrolase and an NAD(P)+-dependent benzaldehyde dehydrogenase from Pseudomonas putida ATCC 12633
    Michael J McLeish
    College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Bacteriol 185:2451-6. 2003
  6. ncbi request reprint Loop movement and catalysis in creatine kinase
    Pan Fen Wang
    College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109, USA
    IUBMB Life 57:355-62. 2005
  7. doi request reprint Physical, kinetic and spectrophotometric studies of a NAD(P)-dependent benzaldehyde dehydrogenase from Pseudomonas putida ATCC 12633
    Catherine K Yeung
    Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, 428 Church Street, Ann Arbor, MI 48109, USA
    Biochim Biophys Acta 1784:1248-55. 2008
  8. ncbi request reprint Mandelamide hydrolase from Pseudomonas putida: characterization of a new member of the amidase signature family
    Kota N Gopalakrishna
    College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109 1065, USA
    Biochemistry 43:7725-35. 2004
  9. pmc Saturation mutagenesis of putative catalytic residues of benzoylformate decarboxylase provides a challenge to the accepted mechanism
    Alejandra Yep
    College of Pharmacy, University of Michigan, 428 Church Street, Ann Arbor, MI 48109 1065, USA
    Proc Natl Acad Sci U S A 105:5733-8. 2008
  10. ncbi request reprint Expression of Torpedo californica creatine kinase in Escherichia coli and purification from inclusion bodies
    Pan Fen Wang
    College of Pharmacy, University of Michigan, 428 Church St, Ann Arbor, MI 48109 1065, USA
    Protein Expr Purif 26:89-95. 2002

Collaborators

  • Michael J McLeish
  • John A Gerlt
  • D J Galas
  • G G Petsko
  • F Jordan
  • Natalia Nemeria
  • Karen N Allen
  • KENNETH ALLEN JOHNSON
  • Pan Fen Wang
  • Alejandra Yep
  • Malea M Kneen
  • Sumit Chakraborty
  • Asim K Bera
  • Walter R P Novak
  • Patricia C Babbitt
  • Gabriel S Brandt
  • Catherine K Yeung
  • Allen J Flynn
  • Miriam S Hasson
  • Petra Siegert
  • Kota N Gopalakrishna
  • Louise Z Wang
  • Elena S Polovnikova
  • Sushmita D Lahiri
  • Dagmar Ringe
  • Juliatek Roestamadji
  • Lena S Polovnikova
  • Theodore S Widlanski
  • Jan H Jensen
  • Kenneth M Merz
  • Guanglei Cui
  • Mor M Naor
  • Martina Pohl
  • Hans Iding
  • Martin Baumann
  • Irina D Pogozheva
  • Adriano D Andricopulo
  • Betty H Stewart
  • John T Burgner
  • Eduard A Sergienko
  • Natalie L Anderson
  • John S Cantwell

Detail Information

Publications23

  1. ncbi request reprint How I became a biochemist
    George L Kenyon
    College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109, USA
    IUBMB Life 57:375-8. 2005
  2. ncbi request reprint Defining the mandate of proteomics in the post-genomics era: workshop report
    George L Kenyon
    College of Pharmacy, University of Michigan, Ann Arbor, MI 48109 1065, USA
    Mol Cell Proteomics 1:763-80. 2002
    ..Looking back 10 to 20 years from now, the question is: Will we have done the job wisely or wastefully? This report summarizes the presentations made at a symposium at the National Academy of Sciences on February 25, 2002...
  3. doi request reprint Using directed evolution to probe the substrate specificity of mandelamide hydrolase
    Pan Fen Wang
    College of Pharmacy, University of Michigan, 428 Church St, Ann Arbor, MI 48109, USA
    Protein Eng Des Sel 22:103-10. 2009
    ..This reduction in activity increased to six orders of magnitude for the G202V variant...
  4. ncbi request reprint Relating structure to mechanism in creatine kinase
    Michael J McLeish
    College of Pharmacy, University of Michigan, Ann Arbor, MI, USA
    Crit Rev Biochem Mol Biol 40:1-20. 2005
    ..This review summarizes and correlates the more significant mechanistic and structural studies on creatine kinase...
  5. pmc Identification and characterization of a mandelamide hydrolase and an NAD(P)+-dependent benzaldehyde dehydrogenase from Pseudomonas putida ATCC 12633
    Michael J McLeish
    College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Bacteriol 185:2451-6. 2003
    ..The mdlY and mdlD genes were isolated and expressed in Escherichia coli, and the purified gene products were characterized as a mandelamide hydrolase and an NAD(P)(+)-dependent benzaldehyde dehydrogenase, respectively...
  6. ncbi request reprint Loop movement and catalysis in creatine kinase
    Pan Fen Wang
    College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109, USA
    IUBMB Life 57:355-62. 2005
    ..Finally, it is clear that neither His66 nor Asp326 are responsible for the pKs observed in the pH-rate profile for HMCK...
  7. doi request reprint Physical, kinetic and spectrophotometric studies of a NAD(P)-dependent benzaldehyde dehydrogenase from Pseudomonas putida ATCC 12633
    Catherine K Yeung
    Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, 428 Church Street, Ann Arbor, MI 48109, USA
    Biochim Biophys Acta 1784:1248-55. 2008
    ..2 to 7.1 while those on the descending limb range from 9.6 to 9.9. A spectrophotometric approach was used to show that the pKa of Cys249 was 8.4, i.e., Cys249 is not responsible for the pKas observed in the pH-rate profiles...
  8. ncbi request reprint Mandelamide hydrolase from Pseudomonas putida: characterization of a new member of the amidase signature family
    Kota N Gopalakrishna
    College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109 1065, USA
    Biochemistry 43:7725-35. 2004
    ..Overall, the MAH data are similar to those of fatty acid amide hydrolase and support the suggestion that there are two distinct subgroups within the amidase signature family...
  9. pmc Saturation mutagenesis of putative catalytic residues of benzoylformate decarboxylase provides a challenge to the accepted mechanism
    Alejandra Yep
    College of Pharmacy, University of Michigan, 428 Church Street, Ann Arbor, MI 48109 1065, USA
    Proc Natl Acad Sci U S A 105:5733-8. 2008
    ....
  10. ncbi request reprint Expression of Torpedo californica creatine kinase in Escherichia coli and purification from inclusion bodies
    Pan Fen Wang
    College of Pharmacy, University of Michigan, 428 Church St, Ann Arbor, MI 48109 1065, USA
    Protein Expr Purif 26:89-95. 2002
    ..The purification described here is in marked contrast with earlier attempts at purification of this isozyme where, in a process yielding less than 1mg/L culture, enzyme with a specific activity of ca. 5U/mg was obtained...
  11. ncbi request reprint Heterogeneity of Escherichia coli-expressed human muscle creatine kinase
    Pan Fen Wang
    College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109, USA
    IUBMB Life 58:421-8. 2006
    ..Two-dimensional IEF analysis showed that an equilibrium was established between the four bands, suggesting that the four components were unstable and generated only when the protein was subjected to IEF...
  12. pmc Exploring the role of the active site cysteine in human muscle creatine kinase
    Pan Fen Wang
    Department of Medicinal Chemistry, University of Michigan, 428 Church Street, Ann Arbor, Michigan 48109, USA
    Biochemistry 45:11464-72. 2006
    ..Finally we use molecular dynamics simulations to demonstrate that, in order to maintain the linear alignment necessary for associative inline transfer of a phosphoryl group, Cys283 needs to be ionized...
  13. ncbi request reprint Exploring the active site of benzaldehyde lyase by modeling and mutagenesis
    Malea M Kneen
    College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, USA
    Biochim Biophys Acta 1753:263-71. 2005
    ..The BAL A28S variant exhibited 7% of WT activity in the BAL assay but, in the most intriguing result, this variant was able to catalyze the decarboxylation of benzoylformate. Conversely, the BFD S26A variant was unable to cleave benzoin...
  14. ncbi request reprint Determinants of substrate specificity in KdcA, a thiamin diphosphate-dependent decarboxylase
    Alejandra Yep
    College of Pharmacy, University of Michigan, 428 Church Street, Ann Arbor, MI 48109 1065, USA
    Bioorg Chem 34:325-36. 2006
    ..The results show that the mutations all lowered the Km value for pyruvate and both the S286Y and F381W variants also had greatly increased values of k(cat) with pyruvate as a substrate...
  15. ncbi request reprint Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida
    Petra Siegert
    Institute of Molecular Enzyme Technology, Heinrich Heine University of Dusseldorf, Research Centre Julich, D 52426 J├╝lich, Germany
    Protein Eng Des Sel 18:345-57. 2005
    ....
  16. ncbi request reprint Structural and kinetic analysis of catalysis by a thiamin diphosphate-dependent enzyme, benzoylformate decarboxylase
    Elena S Polovnikova
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 1392, USA
    Biochemistry 42:1820-30. 2003
    ..H281 is involved in protonation of the enamine. Surprisingly, S26 appears to be involved not only in substrate binding but also in other steps of the reaction...
  17. ncbi request reprint Isoleucine 69 and valine 325 form a specificity pocket in human muscle creatine kinase
    Walter R P Novak
    Department of Biopharmaceutical Sciences, University of California, 600 16 Street, San Francisco, California 94143, USA
    Biochemistry 43:13766-74. 2004
    ..This study enhances our understanding of how the active sites of phosphagen kinases have evolved to recognize their respective substrates and catalyze their reactions...
  18. pmc Probing the active center of benzaldehyde lyase with substitutions and the pseudosubstrate analogue benzoylphosphonic acid methyl ester
    Gabriel S Brandt
    Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454, USA
    Biochemistry 47:7734-43. 2008
    ..We also delineated the role of His29 (the sole potential acid-base catalyst in the active site other than the highly conserved Glu50) and Trp163 in cofactor activation and catalysis by benzaldehyde lyase...
  19. ncbi request reprint Novel mechanism of inhibition of HIV-1 reverse transcriptase by a new non-nucleoside analog, KM-1
    Louise Z Wang
    Department of Chemistry and Biochemistry, Institute of Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA
    J Biol Chem 279:38424-32. 2004
    ..KM-1 can bind to RT in both the absence and presence of DNA but weakens the affinity for DNA 140-fold so that it favors DNA dissociation. The data suggest that KM-1 distorts RT conformation and misaligns DNA at the active site...
  20. doi request reprint Mechanism of benzaldehyde lyase studied via thiamin diphosphate-bound intermediates and kinetic isotope effects
    Sumit Chakraborty
    Department of Chemistry, Rutgers University, Newark, New Jersey 07102, USA
    Biochemistry 47:3800-9. 2008
    ..Neither adduct underwent further reaction. An important finding of the studies is that all thiamin-related intermediates are in a chiral environment on benzaldehyde lyase as reflected by their circular dichroism signatures...
  21. ncbi request reprint Elucidation of the chemistry of enzyme-bound thiamin diphosphate prior to substrate binding: defining internal equilibria among tautomeric and ionization states
    Natalia Nemeria
    Department of Chemistry, Rutgers University, Newark, New Jersey 07102, USA
    Biochemistry 46:10739-44. 2007
    ..The dramatic influence of the protein on the internal equilibria also points to conditions under which the long-elusive ylide intermediate could be stabilized...
  22. ncbi request reprint Mechanism-based inactivation of benzoylformate decarboxylase, a thiamin diphosphate-dependent enzyme
    Asim K Bera
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Am Chem Soc 129:4120-1. 2007
  23. ncbi request reprint The 2.1 A structure of Torpedo californica creatine kinase complexed with the ADP-Mg(2+)-NO(3)(-)-creatine transition-state analogue complex
    Sushmita D Lahiri
    Department of Physiology and Biophysics, Boston University School of Medicine, Boston, Massachusetts 02155, USA
    Biochemistry 41:13861-7. 2002
    ..In addition, the active site of the transition-state analogue complex is completely occluded from solvent, unlike the ADP-Mg(2+)-bound monomer and the unliganded structures reported previously...