Research Topics
| George L KenyonSummaryAffiliation: University of Michigan Country: USA Publications
| Collaborators
|
Detail Information
Publications
Defining the mandate of proteomics in the post-genomics era: workshop reportGeorge L Kenyon
College of Pharmacy, University of Michigan, Ann Arbor, MI 48109 1065, USA
Mol Cell Proteomics 1:763-80. 2002..Looking back 10 to 20 years from now, the question is: Will we have done the job wisely or wastefully? This report summarizes the presentations made at a symposium at the National Academy of Sciences on February 25, 2002...- How I became a biochemistGeorge L Kenyon
College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109, USA
IUBMB Life 57:375-8. 2005 
Using directed evolution to probe the substrate specificity of mandelamide hydrolasePan Fen Wang
College of Pharmacy, University of Michigan, 428 Church St, Ann Arbor, MI 48109, USA
Protein Eng Des Sel 22:103-10. 2009..This reduction in activity increased to six orders of magnitude for the G202V variant...- Relating structure to mechanism in creatine kinaseMichael J McLeish
College of Pharmacy, University of Michigan, Ann Arbor, MI, USA
Crit Rev Biochem Mol Biol 40:1-20. 2005..This review summarizes and correlates the more significant mechanistic and structural studies on creatine kinase... 
Identification and characterization of a mandelamide hydrolase and an NAD(P)+-dependent benzaldehyde dehydrogenase from Pseudomonas putida ATCC 12633Michael J McLeish
College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109, USA
J Bacteriol 185:2451-6. 2003..The mdlY and mdlD genes were isolated and expressed in Escherichia coli, and the purified gene products were characterized as a mandelamide hydrolase and an NAD(P)(+)-dependent benzaldehyde dehydrogenase, respectively...- Loop movement and catalysis in creatine kinasePan-Fen Wang
College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109, USA
IUBMB Life 57:355-62. 2005..Finally, it is clear that neither His66 nor Asp326 are responsible for the pKs observed in the pH-rate profile for HMCK... - Physical, kinetic and spectrophotometric studies of a NAD(P)-dependent benzaldehyde dehydrogenase from Pseudomonas putida ATCC 12633Catherine K Yeung
Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, 428 Church Street, Ann Arbor, MI 48109, USA
Biochim Biophys Acta 1784:1248-55. 2008..2 to 7.1 while those on the descending limb range from 9.6 to 9.9. A spectrophotometric approach was used to show that the pKa of Cys249 was 8.4, i.e., Cys249 is not responsible for the pKas observed in the pH-rate profiles... - Mandelamide hydrolase from Pseudomonas putida: characterization of a new member of the amidase signature familyKota N Gopalakrishna
College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109-1065, USA
Biochemistry 43:7725-35. 2004..Overall, the MAH data are similar to those of fatty acid amide hydrolase and support the suggestion that there are two distinct subgroups within the amidase signature family... - Saturation mutagenesis of putative catalytic residues of benzoylformate decarboxylase provides a challenge to the accepted mechanismAlejandra Yep
College of Pharmacy, University of Michigan, 428 Church Street, Ann Arbor, MI 48109 1065, USA
Proc Natl Acad Sci U S A 105:5733-8. 2008.... - Expression of Torpedo californica creatine kinase in Escherichia coli and purification from inclusion bodiesPan-Fen Wang
College of Pharmacy, University of Michigan, 428 Church St, Ann Arbor, MI 48109-1065, USA
Protein Expr Purif 26:89-95. 2002..The purification described here is in marked contrast with earlier attempts at purification of this isozyme where, in a process yielding less than 1mg/L culture, enzyme with a specific activity of ca. 5U/mg was obtained... - Heterogeneity of Escherichia coli-expressed human muscle creatine kinasePan-Fen Wang
College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109, USA
IUBMB Life 58:421-8. 2006..Two-dimensional IEF analysis showed that an equilibrium was established between the four bands, suggesting that the four components were unstable and generated only when the protein was subjected to IEF... - Exploring the role of the active site cysteine in human muscle creatine kinasePan Fen Wang
Department of Medicinal Chemistry, University of Michigan, 428 Church Street, Ann Arbor, Michigan 48109, USA
Biochemistry 45:11464-72. 2006..Finally we use molecular dynamics simulations to demonstrate that, in order to maintain the linear alignment necessary for associative inline transfer of a phosphoryl group, Cys283 needs to be ionized... - Exploring the active site of benzaldehyde lyase by modeling and mutagenesisMalea M Kneen
College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, USA
Biochim Biophys Acta 1753:263-71. 2005..The BAL A28S variant exhibited 7% of WT activity in the BAL assay but, in the most intriguing result, this variant was able to catalyze the decarboxylation of benzoylformate. Conversely, the BFD S26A variant was unable to cleave benzoin... - Determinants of substrate specificity in KdcA, a thiamin diphosphate-dependent decarboxylaseAlejandra Yep
College of Pharmacy, University of Michigan, 428 Church Street, Ann Arbor, MI 48109-1065, USA
Bioorg Chem 34:325-36. 2006..The results show that the mutations all lowered the Km value for pyruvate and both the S286Y and F381W variants also had greatly increased values of k(cat) with pyruvate as a substrate... - Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putidaPetra Siegert
Institute of Molecular Enzyme Technology, , , , Germany
Protein Eng Des Sel 18:345-57. 2005.... - Structural and kinetic analysis of catalysis by a thiamin diphosphate-dependent enzyme, benzoylformate decarboxylaseElena S Polovnikova
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA
Biochemistry 42:1820-30. 2003..H281 is involved in protonation of the enamine. Surprisingly, S26 appears to be involved not only in substrate binding but also in other steps of the reaction... - Isoleucine 69 and valine 325 form a specificity pocket in human muscle creatine kinaseWalter R P Novak
Department of Biopharmaceutical Sciences, University of California, 600 16 Street, San Francisco, California 94143, USA
Biochemistry 43:13766-74. 2004..This study enhances our understanding of how the active sites of phosphagen kinases have evolved to recognize their respective substrates and catalyze their reactions... - Probing the active center of benzaldehyde lyase with substitutions and the pseudosubstrate analogue benzoylphosphonic acid methyl esterGabriel S Brandt
Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454, USA
Biochemistry 47:7734-43. 2008..We also delineated the role of His29 (the sole potential acid-base catalyst in the active site other than the highly conserved Glu50) and Trp163 in cofactor activation and catalysis by benzaldehyde lyase... - Novel mechanism of inhibition of HIV-1 reverse transcriptase by a new non-nucleoside analog, KM-1Louise Z Wang
Department of Chemistry and Biochemistry, Institute of Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA
J Biol Chem 279:38424-32. 2004..KM-1 can bind to RT in both the absence and presence of DNA but weakens the affinity for DNA 140-fold so that it favors DNA dissociation. The data suggest that KM-1 distorts RT conformation and misaligns DNA at the active site... - Mechanism of benzaldehyde lyase studied via thiamin diphosphate-bound intermediates and kinetic isotope effectsSumit Chakraborty
Department of Chemistry, Rutgers University, Newark, New Jersey 07102, USA
Biochemistry 47:3800-9. 2008..Neither adduct underwent further reaction. An important finding of the studies is that all thiamin-related intermediates are in a chiral environment on benzaldehyde lyase as reflected by their circular dichroism signatures... - Elucidation of the chemistry of enzyme-bound thiamin diphosphate prior to substrate binding: defining internal equilibria among tautomeric and ionization statesNatalia Nemeria
Department of Chemistry, Rutgers University, Newark, New Jersey 07102, USA
Biochemistry 46:10739-44. 2007..The dramatic influence of the protein on the internal equilibria also points to conditions under which the long-elusive ylide intermediate could be stabilized... - Mechanism-based inactivation of benzoylformate decarboxylase, a thiamin diphosphate-dependent enzymeAsim K Bera
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
J Am Chem Soc 129:4120-1. 2007 - The 2.1 A structure of Torpedo californica creatine kinase complexed with the ADP-Mg(2+)-NO(3)(-)-creatine transition-state analogue complexSushmita D Lahiri
Department of Physiology and Biophysics, Boston University School of Medicine, Boston, Massachusetts 02155, USA
Biochemistry 41:13861-7. 2002..In addition, the active site of the transition-state analogue complex is completely occluded from solvent, unlike the ADP-Mg(2+)-bound monomer and the unliganded structures reported previously...