Hans Keirstead

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint Stem cell transplantation into the central nervous system and the control of differentiation
    H S Keirstead
    Reeve Irvine Research Center, Department of Anatomy and Neurobiology, University of California, Irvine, California 92697 4292, USA
    J Neurosci Res 63:233-6. 2001
  2. ncbi request reprint Transplantation of glial-committed progenitor cells into a viral model of multiple sclerosis induces remyelination in the absence of an attenuated inflammatory response
    Jenny L Hardison
    Department of Molecular Biology and Biochemistry, 3205 McGaugh Hall, University of California, Irvine, 92697 3900, USA
    Exp Neurol 197:420-9. 2006
  3. ncbi request reprint Cellular therapies in motor neuron diseases
    Mamatha S Nayak
    Department of Neurology, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, MD 21287, USA
    Biochim Biophys Acta 1762:1128-38. 2006
  4. ncbi request reprint Therapeutic neutralization of CXCL10 decreases secondary degeneration and functional deficit after spinal cord injury in mice
    Rafael Gonzalez
    University of California, Reeve Irvine Research Center, Department of Anatomy and Neurobiology, 2111 Gillespie Neuroscience Research Facility, College of Medicine, Irvine, CA 92697 4292, USA
    Regen Med 2:771-83. 2007
  5. pmc Histological and functional benefit following transplantation of motor neuron progenitors to the injured rat spinal cord
    Sharyn L Rossi
    Department of Anatomy and Neurobiology, School of Medicine, Reeve Irvine Research Center, University of California at Irvine, Irvine, California, United States of America
    PLoS ONE 5:e11852. 2010
  6. pmc Derivation of high purity neuronal progenitors from human embryonic stem cells
    Gabriel Nistor
    Reeve Irvine Research Center, Department of Anatomy and Neurobiology, School of Medicine, Sue and Bill Gross Hall, a CIRM Institute, University of California Irvine, Irvine, California, United States of America
    PLoS ONE 6:e20692. 2011
  7. pmc Immunological demyelination triggers macrophage/microglial cells activation without inducing astrogliosis
    Frank Cloutier
    Reeve Irvine Research Center, Sue and Bill Gross Stem Cell Research Center, School of Medicine, University of California at Irvine, 845 Health Sciences Road, Irvine, CA 92697, USA Département de Biologie de l Université de Moncton, 18 Antonine Maillet, Moncton, NB, Canada E1A 3E9
    Clin Dev Immunol 2013:812456. 2013
  8. pmc Equivalence of conventionally-derived and parthenote-derived human embryonic stem cells
    Julie V Harness
    Reeve Irvine Research Center, Sue and Bill Gross Stem Cell Research Center, Department of Anatomy and Neurobiology, School of Medicine, University of California at Irvine, Irvine, California, United States of America
    PLoS ONE 6:e14499. 2011
  9. pmc Human motor neuron progenitor transplantation leads to endogenous neuronal sparing in 3 models of motor neuron loss
    Tanya J Wyatt
    Department of Anatomy and Neurobiology, Reeve Irvine Research Center, Sue and Bill Gross Stem Cell Research Center, School of Medicine, 2002 Sue and Bill Gross Hall A CIRM Institute, University of California at Irvine, Irvine, CA 92697 4292, USA
    Stem Cells Int 2011:207230. 2011
  10. pmc Ascending central canal dilation and progressive ependymal disruption in a contusion model of rodent chronic spinal cord injury
    Milan Radojicic
    Reeve Irvine Research Center, Department of Anatomy and Neurobiology, University of California at Irvine, 2111 Gillespie Neuroscience Research Facility, Irvine, CA 92697 4292, USA
    BMC Neurol 7:30. 2007

Research Grants

Collaborators

Detail Information

Publications42

  1. ncbi request reprint Stem cell transplantation into the central nervous system and the control of differentiation
    H S Keirstead
    Reeve Irvine Research Center, Department of Anatomy and Neurobiology, University of California, Irvine, California 92697 4292, USA
    J Neurosci Res 63:233-6. 2001
    ....
  2. ncbi request reprint Transplantation of glial-committed progenitor cells into a viral model of multiple sclerosis induces remyelination in the absence of an attenuated inflammatory response
    Jenny L Hardison
    Department of Molecular Biology and Biochemistry, 3205 McGaugh Hall, University of California, Irvine, 92697 3900, USA
    Exp Neurol 197:420-9. 2006
    ....
  3. ncbi request reprint Cellular therapies in motor neuron diseases
    Mamatha S Nayak
    Department of Neurology, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, MD 21287, USA
    Biochim Biophys Acta 1762:1128-38. 2006
    ..We review pre-clinical data in rodents with each of these approaches and discuss advances and regulatory issues regarding the use of cellular therapies in human motor neuron diseases...
  4. ncbi request reprint Therapeutic neutralization of CXCL10 decreases secondary degeneration and functional deficit after spinal cord injury in mice
    Rafael Gonzalez
    University of California, Reeve Irvine Research Center, Department of Anatomy and Neurobiology, 2111 Gillespie Neuroscience Research Facility, College of Medicine, Irvine, CA 92697 4292, USA
    Regen Med 2:771-83. 2007
    ..Notably, this therapeutic treatment also promoted revascularization of the injured spinal cord and functional recovery. These data suggest that anti-CXCL10 antibody treatment is a viable therapeutic strategy for acute SCI...
  5. pmc Histological and functional benefit following transplantation of motor neuron progenitors to the injured rat spinal cord
    Sharyn L Rossi
    Department of Anatomy and Neurobiology, School of Medicine, Reeve Irvine Research Center, University of California at Irvine, Irvine, California, United States of America
    PLoS ONE 5:e11852. 2010
    ..Motor neuron loss is characteristic of cervical spinal cord injury (SCI) and contributes to functional deficit...
  6. pmc Derivation of high purity neuronal progenitors from human embryonic stem cells
    Gabriel Nistor
    Reeve Irvine Research Center, Department of Anatomy and Neurobiology, School of Medicine, Sue and Bill Gross Hall, a CIRM Institute, University of California Irvine, Irvine, California, United States of America
    PLoS ONE 6:e20692. 2011
    ..Our method of deriving neuronal progenitors from hESCs renders the process amenable to therapeutic and commercial use...
  7. pmc Immunological demyelination triggers macrophage/microglial cells activation without inducing astrogliosis
    Frank Cloutier
    Reeve Irvine Research Center, Sue and Bill Gross Stem Cell Research Center, School of Medicine, University of California at Irvine, 845 Health Sciences Road, Irvine, CA 92697, USA Département de Biologie de l Université de Moncton, 18 Antonine Maillet, Moncton, NB, Canada E1A 3E9
    Clin Dev Immunol 2013:812456. 2013
    ....
  8. pmc Equivalence of conventionally-derived and parthenote-derived human embryonic stem cells
    Julie V Harness
    Reeve Irvine Research Center, Sue and Bill Gross Stem Cell Research Center, Department of Anatomy and Neurobiology, School of Medicine, University of California at Irvine, Irvine, California, United States of America
    PLoS ONE 6:e14499. 2011
    ..The objective of this work was to determine points of equivalence and differences between conventionally-derived hESC and parthenote-derived hESC lines (phESC) in the undifferentiated state and during neural differentiation...
  9. pmc Human motor neuron progenitor transplantation leads to endogenous neuronal sparing in 3 models of motor neuron loss
    Tanya J Wyatt
    Department of Anatomy and Neurobiology, Reeve Irvine Research Center, Sue and Bill Gross Stem Cell Research Center, School of Medicine, 2002 Sue and Bill Gross Hall A CIRM Institute, University of California at Irvine, Irvine, CA 92697 4292, USA
    Stem Cells Int 2011:207230. 2011
    ..The ability to maintain dying motor neurons by delivering motor neuron-specific neurotrophic support represents a powerful treatment strategy for diseases characterized by motor neuron loss...
  10. pmc Ascending central canal dilation and progressive ependymal disruption in a contusion model of rodent chronic spinal cord injury
    Milan Radojicic
    Reeve Irvine Research Center, Department of Anatomy and Neurobiology, University of California at Irvine, 2111 Gillespie Neuroscience Research Facility, Irvine, CA 92697 4292, USA
    BMC Neurol 7:30. 2007
    ..At present, no pathological mechanisms satisfactorily explain the ongoing degeneration...
  11. ncbi request reprint Stem cells for the treatment of myelin loss
    Hans S Keirstead
    Reeve Irvine Research Center, Department of Anatomy and Neurobiology, School of Medicine, 2111 Gillespie Neuroscience Research Facility, University of California at Irvine, Irvine, CA 92697 4292, USA
    Trends Neurosci 28:677-83. 2005
    ..Here, I present the emerging perspective that stem-cell-mediated remyelination of the adult CNS is a viable therapeutic strategy, and discuss the challenges to remyelination posed by the environment of acute and chronic injuries...
  12. ncbi request reprint A noninvasive ultrasonographic method to evaluate bladder function recovery in spinal cord injured rats
    Hans S Keirstead
    Reeve Irvine Research Center, Department of Anatomy and Neurobiology, 2111 Gillespie Neuroscience Research Facility, College of Medicine, University of California Irvine, Irvine, CA 92697 4292, USA
    Exp Neurol 194:120-7. 2005
    ....
  13. ncbi request reprint Human embryonic stem cell-derived oligodendrocyte progenitor cell transplants remyelinate and restore locomotion after spinal cord injury
    Hans S Keirstead
    Department of Anatomy and Neurobiology, Reeve Irvine Research Center, College of Medicine, University of California at Irvine, Irvine, California 92697 4292, USA
    J Neurosci 25:4694-705. 2005
    ..These studies document the feasibility of predifferentiating hESCs into functional OPCs and demonstrate their therapeutic potential at early time points after spinal cord injury...
  14. doi request reprint Safety assessment of myogenic stem cell transplantation and resulting tumor formation
    Stephanie A Jacobs
    From the Division of Urogynecology, Department of Obstetrics and Gynecology, University of California, Irvine Medical Center, Orange, CA and Department of Anatomy and Neurobiology Reeve Irvine Research Center, University of California Irvine, Irvine, CA
    Female Pelvic Med Reconstr Surg 19:362-8. 2013
    ..To assess for stem cell migration to liver and lung after transplantation in injured rat anal sphincters. To evaluate histological findings of unanticipated ectopic foci of growth...
  15. pmc Human embryonic stem cell-derived oligodendrocyte progenitor cell transplants improve recovery after cervical spinal cord injury
    Jason Sharp
    Reeve Irvine Research Center, Sue and Bill Gross Stem Cell Research Center, Department of Anatomy and Neurobiology, School of Medicine, 2111 Gillespie Neuroscience Research Facility, University of California at Irvine, Irvine, California 92697 4292, USA
    Stem Cells 28:152-63. 2010
    ..These findings further our understanding of the histopathology and functional outcomes of cervical SCI, define potential therapeutic targets, and support the use of these cells as a treatment for cervical SCI...
  16. ncbi request reprint Remyelination, axonal sparing, and locomotor recovery following transplantation of glial-committed progenitor cells into the MHV model of multiple sclerosis
    Minodora O Totoiu
    Reeve Irvine Research Center, Department of Anatomy and Neurobiology, College of Medicine, University of California at Irvine, 2111 Gillespie Neuroscience Research Facility, Irvine, CA 92697 4292, USA
    Exp Neurol 187:254-65. 2004
    ..This study demonstrates for the first time that transplant-mediated remyelination is possible in the pathogenic environment of the MHV demyelination model and that it is associated with locomotor improvement...
  17. ncbi request reprint Endogenous neurogenesis replaces oligodendrocytes and astrocytes after primate spinal cord injury
    Hong Yang
    Department of Neurosciences, University of California, San Diego, La Jolla, California 92093 0626, USA
    J Neurosci 26:2157-66. 2006
    ..Thus, cell replacement is an extensive natural compensatory response to injury in the primate spinal cord that contributes to neural repair and is a potential target for therapeutic enhancement...
  18. doi request reprint In vivo recovery of the injured anal sphincter after repair and injection of myogenic stem cells: an experimental model
    Felicia L Lane
    1 Division of Urogynecology, Department of Obstetrics and Gynecology, University of California Irvine, Orange, California 2 Department of Anatomy and Neurobiology, Reeve Irvine Research Center, University of California Irvine, Orange, California
    Dis Colon Rectum 56:1290-7. 2013
    ..This study aims to evaluate in vivo function of the external anal sphincter after transection and repair augmented with myogenic stem cells, and to establish normative electromyography parameters of the rodent external anal sphincter...
  19. ncbi request reprint Combined demyelination plus Schwann cell transplantation therapy increases spread of cells and axonal regeneration following contusion injury
    Roya Azanchi
    Reeve Irvine Research Center, Department of Anatomy and Neurobiology, College of Medicine, University of California at Irvine, 92697 4292, USA
    J Neurotrauma 21:775-88. 2004
    ..These findings suggest that this combination therapy may overcome a central limitation of transplant strategies in which the permissive environment provided remains at the implantation site...
  20. ncbi request reprint Spinal cord injury is accompanied by chronic progressive demyelination
    Minodora O Totoiu
    Reeve Irvine Research Center, Department of Anatomy and Neurobiology, University of California at Irvine, 92697 4292, USA
    J Comp Neurol 486:373-83. 2005
    ..These studies demonstrate for the first time that spinal cord injury is accompanied by chronic progressive demyelination, and they substantiate demyelination as a target for therapeutic intervention...
  21. ncbi request reprint Stem cells for the treatment of spinal cord injury
    Margaret Coutts
    Reeve Irvine Research Center, Stem Cell Research Center, Department of Anatomy and Neurobiology, College of Medicine, University of California Irvine, Irvine, CA, USA
    Exp Neurol 209:368-77. 2008
    ..With a growing understanding of these practical issues, stem cell biology, and spinal cord injury pathophysiology, stem cell-based therapies are moving closer to clinical application...
  22. ncbi request reprint Derivation of oligodendrocyte progenitor cells from human embryonic stem cells
    Jason Sharp
    Keirstead Research Group, Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA, USA
    Methods Mol Biol 767:399-409. 2011
    ..Here, we describe a protocol to generate oligodendroglial lineage-specific cells in high purity from human embryonic stem cells...
  23. doi request reprint A novel model to measure the regenerative potential of the peripheral nervous system after experimental immunological demyelination
    Aaron M Kosins
    Department of Tissue Engineering and Regenerative Medicine, Reeve Irvine Research Center, Aesthetic and Plastic Surgery Institute, University of California, Irvine, Calif 92868, USA
    Plast Reconstr Surg 123:1688-96. 2009
    ....
  24. pmc Myelin pathogenesis and functional deficits following SCI are age-associated
    Monica M Siegenthaler
    Reeve Irvine Research Center, Sue and Bill Gross Stem Cell Research Center, Department of Anatomy and Neurobiology, School of Medicine, University of California at Irvine, Irvine, CA 92697 4292, USA
    Exp Neurol 213:363-71. 2008
    ....
  25. ncbi request reprint Therapeutic applications of oligodendrocyte precursors derived from human embryonic stem cells
    Jason Sharp
    Reeve Irvine Research Center, Department of Anatomy and Neurobiology, University of California at Irvine, 2111 Gillespie Neuroscience Research Facility, Irvine, CA 92697 4292, United States
    Curr Opin Biotechnol 18:434-40. 2007
  26. ncbi request reprint Assessment of behavior in animal models of spinal cord injury
    J Patrick Kesslak
    Institute for Brain Aging and Dementia, Department of Neurology, University of California at Irvine, Irvine, California 92697 4540, USA
    J Spinal Cord Med 26:323-8. 2003
    ..The appropriate use and interpretation of these methods is critical for proper study design, interpretation of experimental results, and extrapolation to clinical relevance...
  27. ncbi request reprint Human embryonic stem cells differentiate into oligodendrocytes in high purity and myelinate after spinal cord transplantation
    Gabriel I Nistor
    Reeve Irvine Research Center, Department of Anatomy and Neurobiology, 2111 Gillespie Neuroscience Research Facility, Gillespie Neuroscience Research Facility, College of Medicine, University of California at Irvine, Irvine, CA 92697, USA
    Glia 49:385-96. 2005
    ....
  28. ncbi request reprint Transplantation of human embryonic stem cell-derived oligodendrocyte progenitors into rat spinal cord injuries does not cause harm
    Frank Cloutier
    University of California at Irvine, Reeve Irvine Research Center, Department of Anatomy, College of Medicine, 2111 Gillespie Neuroscience Research Facility, Irvine, CA 92697 4292, USA
    Regen Med 1:469-79. 2006
    ..In addition, our results indicate that transplantation of human embryonic stem cell-derived oligodendrocyte progenitor cells into the injured spinal cord is not associated with a decline in locomotor function...
  29. pmc Voluntary running attenuates age-related deficits following SCI
    Monica M Siegenthaler
    Reeve Irvine Research Center, Department of Anatomy and Neurobiology, School of Medicine, University of California at Irvine, Irvine, CA 92697 4292, USA
    Exp Neurol 210:207-16. 2008
    ..Importantly, our data indicate that exercise attenuates these age-related deficits following SCI...
  30. ncbi request reprint Neutralization of the chemokine CXCL10 reduces apoptosis and increases axon sprouting after spinal cord injury
    Janette Glaser
    Department of Anatomy and Neurobiology, Reeve Irvine Research Center, University of California at Irvine, 92697 4292, USA
    J Neurosci Res 84:724-34. 2006
    ..These findings indicate that anti-CXCL10 antibody treatment provides an environment that reduces apoptosis and increases axon sprouting following injury to the adult spinal cord...
  31. ncbi request reprint Stem cells and spinal cord regeneration
    Sharyn L Rossi
    Reeve Irvine Research Center, Sue and Bill Gross Stem Cell Research Center, Department of Anatomy and Neurobiology, School of Medicine, 2111 Gillespie Neuroscience Research Facility, University of California at Irvine, Irvine, CA 92697 4292, USA
    Curr Opin Biotechnol 20:552-62. 2009
    ..High purity populations derived from stem cells will serve to better delineate lineage-specific mechanisms of repair, while providing both neurotrophic and phenotypic benefits to the injured spinal cord...
  32. doi request reprint Endogenous remyelination is induced by transplant rejection in a viral model of multiple sclerosis
    Maya N Hatch
    Reeve Irvine Research Center, Department of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, CA 92697 4292, United States
    J Neuroimmunol 212:74-81. 2009
    ..These data suggest that remyelination was initiated by the local response to xenograft transplantation. These findings illustrate the complexities of OPC transplantation into areas of robust immune-mediated pathology...
  33. doi request reprint Stem cell-based cell replacement strategies for the central nervous system
    Jason Sharp
    Reeve Irvine Research Center, Sue and Bill Gross Stem Cell Research Center, Department of Anatomy and Neurobiology, School of Medicine, 2111 Gillespie Neuroscience Research Facility, University of California at Irvine, Irvine, CA 92697 4292, United States
    Neurosci Lett 456:107-11. 2009
    ..Here we present emerging perspectives on the development of neuronal and glial cells from stem cells for clinical application to CNS diseases and injury...
  34. ncbi request reprint The extent of myelin pathology differs following contusion and transection spinal cord injury
    Monica M Siegenthaler
    Reeve Irvine Research Center, Departments of Anatomy and Neurobiology, College of Medicine, University of California at Irvine, Irvine, California 92697 4292, USA
    J Neurotrauma 24:1631-46. 2007
    ..These data indicate for the first time that myelin pathology differs substantially following contusion and transection SCI...
  35. doi request reprint Derivation of high-purity oligodendroglial progenitors
    Maya N Hatch
    Department of Anatomy and Neurobiology, Reeve Irvine Research Center, University of California at Irvine, Irvine, CA, USA
    Methods Mol Biol 549:59-75. 2009
    ..This chapter outlines methods of generating high-purity OPCs from multipotent neonatal progenitor or human embryonic stem cells...
  36. ncbi request reprint Allogenic myoblast transplantation in the rat anal sphincter
    Jocelyn B Craig
    From the Division of Urogynecology, Department of Obstetrics and Gynecology, and Department of Anatomy and Neurobiology, Reeve Irvine Research Center, University of California Irvine, Orange, CA
    Female Pelvic Med Reconstr Surg 16:205-8. 2010
    ..To determine the feasibility of injecting rat myoblasts into the intact anal sphincter as a potential treatment for anal incontinence, and to detect transferred myoblast survival and integration...
  37. ncbi request reprint Reducing inflammation decreases secondary degeneration and functional deficit after spinal cord injury
    Rafael Gonzalez
    Reeve Irvine Research Center, Department of Anatomy and Neurobiology, University of California at Irvine, 2111 Gillespie Neuroscience Research Facility, Irvine, CA 92697 4292, USA
    Exp Neurol 184:456-63. 2003
    ..We conclude that CXCL10 plays a critical role in recruitment of T lymphocytes to sites of spinal cord injury, and that a reduction of T-lymphocyte recruitment significantly enhances tissue preservation and functional outcome...
  38. ncbi request reprint Septations in chronic spinal cord injury cavities contain axons
    Milan Radojicic
    Reeve Irvine Research Center, Department of Anatomy and Neurobiology, 2111 Gillespie Neuroscience Research Facility, College of Medicine, University of California Irvine, Irvine, CA 92697 4292, USA
    Exp Neurol 196:339-41. 2005
    ..Here, we show for the first time in a rodent model of chronic SCI that the septations spanning cavities contain axons...
  39. ncbi request reprint Neutralization of the chemokine CXCL10 enhances tissue sparing and angiogenesis following spinal cord injury
    Janette Glaser
    Department of Anatomy and Neurobiology, Reeve Irvine Research Center, University of California at Irvine, Irvine, California 92697, USA
    J Neurosci Res 77:701-8. 2004
    ..Improved blood flow and oxygen supply to the injury site may contribute to the functional improvement associated with this treatment...
  40. doi request reprint Trophic factors GDNF and BDNF improve function of retinal sheet transplants
    Pamela B Yang
    Anatomy and Neurobiology, Univ of California, Irvine, CA, USA
    Exp Eye Res 91:727-38. 2010
    ..In conclusion, treatment of retinal transplants with GDNF and BDNF improved the restoration of visual responses in RD rats; and GDNF appears to exert greater overall restoration than BDNF...
  41. pmc Three-dimensional optical coherence tomography imaging of retinal sheet implants in live rats
    Magdalene J Seiler
    Department of Anatomy and Neurobiology, University of California, Reeve Irvine Research Center, Sue and Bill Gross Stem Cell Research Center, Irvine, CA, United States
    J Neurosci Methods 188:250-7. 2010
    ..To obtain three-dimensional images from retinal transplants in live animals and evaluate the placement and structural quality of the transplants...
  42. ncbi request reprint Three-dimensional early retinal progenitor 3D tissue constructs derived from human embryonic stem cells
    Gabriel Nistor
    Department of Anatomy and Neurobiology, Reeve Irvine Research Center, Sue and Bill Gross Stem Cell Research Center, University of California at Irvine, Irvine, CA, USA
    J Neurosci Methods 190:63-70. 2010
    ..To develop three-dimensional (3D) constructs of retinal pigment epithelium (RPE) and early retina progenitor cells from human embryonic stem cells (hESCs)...

Research Grants5

  1. Demyelination Plus Transplantation to Study CNS Repair
    Hans Keirstead; Fiscal Year: 2002
    ..abstract_text> ..
  2. Demyelination Plus Transplantation to Study CNS Repair
    Hans Keirstead; Fiscal Year: 2003
    ..abstract_text> ..
  3. Demyelination Plus Transplantation to Study CNS Repair
    Hans Keirstead; Fiscal Year: 2004
    ..abstract_text> ..
  4. Demyelination Plus Transplantation to Study CNS Repair
    Hans Keirstead; Fiscal Year: 2005
    ..abstract_text> ..
  5. Demyelination Plus Transplantation to Study CNS Repair
    Hans Keirstead; Fiscal Year: 2006
    ..abstract_text> ..