Research Topics
| Jay KeaslingSummaryAffiliation: University of California Country: USA Publications
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Publications
Synthetic biology and the development of tools for metabolic engineeringJay D Keasling
Synthetic Biology Engineering Research Center, University of California, Berkeley, CA 94720, USA
Metab Eng 14:189-95. 2012..The development of these tools significantly reduced the costs and time to develop the antimalarial drug artemisinin, but the availability of more tools could have reduced these costs substantially...
Supplementation of intracellular XylR leads to coutilization of hemicellulose sugarsDan Groff
Joint BioEnergy Institute, Emeryville, California, USA
Appl Environ Microbiol 78:2221-9. 2012..This strain is a useful starting point for the development of an E. coli biocatalyst that can simultaneously convert all biomass sugars...- BglBricks: A flexible standard for biological part assemblyJ Christopher Anderson
Department of Bioengineering, University of California, Berkeley, CA 94720, USA
J Biol Eng 4:1. 2010..These tools will provide a foundation from which to transform genetic engineering from a technically intensive art into a purely design-based discipline... - Central metabolism in Mycobacterium smegmatis during the transition from O2-rich to O2-poor conditions as studied by isotopomer-assisted metabolite analysisYinjie J Tang
Department of Energy, Environmental and Chemical Engineering, Washington University, St Louis, MO 63130, USA
Biotechnol Lett 31:1233-40. 2009.... - Global analysis of host response to induction of a latent bacteriophageRobin E Osterhout
Department of Bioengineering and Howard Hughes Medical Institute, University of California at Berkeley, Berkeley, CA 94720, USA
BMC Microbiol 7:82. 2007..To address this question, we collected transcriptional profiles of Escherichia coli and bacteriophage lambda throughout lysogenic induction by UV light... - Induction of multiple pleiotropic drug resistance genes in yeast engineered to produce an increased level of anti-malarial drug precursor, artemisinic acidDae Kyun Ro
Department of Biological Sciences, University of Calgary, Calgary, T2N 1N4, Canada
BMC Biotechnol 8:83. 2008..annua. In order to develop an industrially competent yeast strain, detailed analyses of microbial physiology and development of gene expression strategies are required... - A rapid and inexpensive labeling method for microarray gene expression analysisMario Ouellet
The Joint Bioenergy Institute, Lawrence Berkeley National Laboratory, Emeryville, USA
BMC Biotechnol 9:97. 2009.... - Manufacturing molecules through metabolic engineeringJay D Keasling
Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA 94608, USA
Science 330:1355-8. 2010..In any future, metabolic engineering will soon rival and potentially eclipse synthetic organic chemistry... - A framework and model system to investigate linear system behavior in Escherichia coliMeghdad Hajimorad
Synthetic Biology Engineering Research Center, University of California, Berkeley, CA 94720, USA
J Biol Eng 5:3. 2011..abstract:.. - Synthetic biology for synthetic chemistryJay D Keasling
Department of Chemical Engineering, University of California, Berkeley, Berkeley, California 94720, USA
ACS Chem Biol 3:64-76. 2008..Some of the most important tools for engineering bacterial metabolism and their use for production of the antimalarial drug artemisinin are reviewed... - Modular engineering of L-tyrosine production in Escherichia coliDarmawi Juminaga
California Institute for Quantitative Biosciences and Berkeley Center for Synthetic Biology, University of California, Berkeley, California, USA
Appl Environ Microbiol 78:89-98. 2012..This work demonstrates the utility of targeted proteomics and metabolite profiling in pathway construction and optimization, which should be applicable to other metabolic pathways... - Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanolEric J Steen
Joint BioEnergy Institute, 5885 Hollis Avenue, Emeryville, CA 94608, USA
Microb Cell Fact 7:36. 2008..coli. Using metabolite analysis, we were able to determine which steps in the n-butanol biosynthetic pathway were the most problematic and ripe for future improvement... 
Engineering Escherichia coli for production of functionalized terpenoids using plant P450sMichelle C Y Chang
California Institute for Quantitative Biomedical Research, University of California, Berkeley, Berkeley, California 94720 3224, USA
Nat Chem Biol 3:274-7. 2007..coli at high titer using native plant P450s...- Balancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coliDouglas J Pitera
Department of Chemical Engineering, University of California, Berkeley, CA 94720 1462, USA
Metab Eng 9:193-207. 2007..These results demonstrate that balancing carbon flux through the heterologous pathway is a key determinant in optimizing isoprenoid biosynthesis in microbial hosts... - Production of the antimalarial drug precursor artemisinic acid in engineered yeastDae-Kyun Ro
California Institute of Quantitative Biomedical Research, Lawrence Berkeley National Laboratory, University of California, Berkeley, California 94720, USA
Nature 440:940-3. 2006..annua, yield optimization and industrial scale-up will be required to raise artemisinic acid production to a level high enough to reduce artemisinin combination therapies to significantly below their current prices... - Engineering a mevalonate pathway in Escherichia coli for production of terpenoidsVincent J J Martin
Department of Chemical Engineering, 201 Gilman Hall, University of California, Berkeley, California 94720-1462, USA
Nat Biotechnol 21:796-802. 2003.... - Identification of isopentenol biosynthetic genes from Bacillus subtilis by a screening method based on isoprenoid precursor toxicitySydnor T Withers
Department of Chemical Engineering, University of California, Berkeley, California, USA
Appl Environ Microbiol 73:6277-83. 2007..Expression of nudF in E. coli engineered with the mevalonate-based isopentenyl pyrophosphate biosynthetic pathway resulted in the production of isopentenol... - Heterologous protein production in Escherichia coli using the propionate-inducible pPro system by conventional and auto-induction methodsSung Kuk Lee
Department of Chemical Engineering, University of California, Berkeley, CA 94720, USA
Protein Expr Purif 61:197-203. 2008..These results strongly indicate that the pPro system is well-suited for overproduction of recombinant proteins... - High-throughput metabolic engineering: advances in small-molecule screening and selectionJeffrey A Dietrich
UCSF UCB Joint Graduate Group in Bioengineering, Berkeley, California 94720, USA
Annu Rev Biochem 79:563-90. 2010..We conclude by providing our prospectus for the future, focusing on transcription factor-based detection systems as a natural microbial mode of small-molecule detection... - Synthetic protein scaffolds provide modular control over metabolic fluxJohn E Dueber
California Institute of Quantitative Biomedical Research QB3, University of California, Berkeley, California, USA
Nat Biotechnol 27:753-9. 2009..5 g/l) without the synthetic complex. These strategies should prove generalizeable to other metabolic pathways and programmable for fine-tuning pathway flux... - Effects of the sequence and size of non-polar residues on self-assembly of amphiphilic peptidesKai Wang
Department of Chemical Engineering, University of California, Berkeley, CA 94720, USA
Int J Biol Macromol 36:232-40. 2005..The sequence and steric size of non-polar residues are shown to cause variations in peptide secondary structures and create significant differences in the matrix morphology of self-assembled peptides... - Effect of glucose or glycerol as the sole carbon source on gene expression from the Salmonella prpBCDE promoter in Escherichia coliSung Kuk Lee
Department of Chemical Engineering, University of California, USA
Biotechnol Prog 22:1547-51. 2006..The expression system is comparable to the widely used T7 promoter-driven expression systems in glucose or glycerol minimal medium... - Biosynthesis of plant isoprenoids: perspectives for microbial engineeringJames Kirby
California Institute of Quantitative Biomedical Research, University of California, Berkeley, California 94720, USA
Annu Rev Plant Biol 60:335-55. 2009..Here, we review investigations into isoprenoid function and gene discovery in plants as well as the latest advances in isoprenoid pathway engineering in both plant and microbial hosts... - Engineering of the pyruvate dehydrogenase bypass in Saccharomyces cerevisiae for high-level production of isoprenoidsYoichiro Shiba
Berkeley Center for Synthetic Biology, Department of Chemical Engineering, University of California, 717 Potter Street, Building 977, Mail code 3224, Berkeley, CA 94720 3224, USA
Metab Eng 9:160-8. 2007..This work will be generally applicable to the production of a broad range of isoprenoids in yeast... - Production of isoprenoid pharmaceuticals by engineered microbesMichelle C Y Chang
California Institute for Quantitative Biomedical Research, University of California-Berkeley, Berkeley, CA 94720, USA
Nat Chem Biol 2:674-81. 2006..In this Perspective, we discuss ongoing research aimed toward the production of terpenoid natural products in genetically engineered Escherichia coli and Saccharomyces cerevisiae... - Metabolic engineering of microorganisms for isoprenoid productionJames Kirby
California Institute of Quantitative Biomedical Research, University of California, Berkeley, CA 94720, USA
Nat Prod Rep 25:656-61. 2008..Here we review recent advances in the metabolic engineering of microbes for isoprenoid production as well as some novel approaches to gene discovery and expression... - High-level production of amorpha-4,11-diene in a two-phase partitioning bioreactor of metabolically engineered Escherichia coliJack D Newman
Department of Chemical Engineering, University of California, Berkeley, California, USA
Biotechnol Bioeng 95:684-91. 2006..5 g/L of culture medium. A standard of amorphadiene collected from the off-gas showed that the caryophyllene standard overestimated amorphadiene production by approximately 30%... - Optimization of the mevalonate-based isoprenoid biosynthetic pathway in Escherichia coli for production of the anti-malarial drug precursor amorpha-4,11-dieneJennifer R Anthony
California Institute for Quantitative Biomedical Research, University of California, Berkeley, CA 94720, USA
Metab Eng 11:13-9. 2009..The methods demonstrated here are applicable for identifying and eliminating rate-limiting steps in other constructed biosynthetic pathways... - Mono and diterpene production in Escherichia coliK Kinkead Reiling
Department of Chemical Engineering, The University of California Berkeley, California 94720 1462, USA
Biotechnol Bioeng 87:200-12. 2004..Construction and functional expression of GGPP and GPP operons provides an in vivo precursor platform host for the future engineering of di- and monoterpene cyclases and the overproduction of terpenes in bacteria... - Optimization of DsRed production in Escherichia coli: effect of ribosome binding site sequestration on translation efficiencyBrian F Pfleger
Department of Chemical Engineering, University of California Berkeley, California, USA
Biotechnol Bioeng 92:553-8. 2005..The new DsRed, designated here as RFP(EC) for E. coli optimized red fluorescent protein, fluoresces 2.5 times greater than DsRed-Express when expressed from the same vector... - A propionate-inducible expression system for enteric bacteriaSung Kuk Lee
Department of Chemical Engineering, University of California, Berkeley, CA 94720, USA
Appl Environ Microbiol 71:6856-62. 2005..Since the prpBCDE promoter has CAP-dependent activation, the prpR-P(prpB) system exhibited negligible basal expression by addition of glucose to the medium... - A novel semi-biosynthetic route for artemisinin production using engineered substrate-promiscuous P450(BM3)Jeffrey A Dietrich
UCSF UCB Joint Graduate Group in Bioengineering, University of California at Berkeley, Berkeley, CA 94720, USA
ACS Chem Biol 4:261-7. 2009..coli. We also demonstrate high-yielding, selective transformations to dihydroartemisinic acid, the immediate precursor to the high-value antimalarial drug artemisinin... - Directed evolution of AraC for improved compatibility of arabinose- and lactose-inducible promotersSung Kuk Lee
Department of Chemical Engineering, University of California, Berkeley, California 94720, USA
Appl Environ Microbiol 73:5711-5. 2007..Detailed studies indicate that the AraC dimerization domain and C terminus are important for the increased sensitivity of AraC to arabinose... - Functional genomic study of exogenous n-butanol stress in Escherichia coliBecky J Rutherford
Department of Chemical Engineering, University of California, Berkeley, CA 94720, USA
Appl Environ Microbiol 76:1935-45. 2010..Cellular engineering based on these cues may assist in developing a high-titer, n-butanol-producing host... - Microbial production of fatty-acid-derived fuels and chemicals from plant biomassEric J Steen
Joint BioEnergy Institute, Emeryville, California 94608, USA
Nature 463:559-62. 2010..Furthermore, we show engineering of the biodiesel-producing cells to express hemicellulases, a step towards producing these compounds directly from hemicellulose, a major component of plant-derived biomass... - Genes involved in long-chain alkene biosynthesis in Micrococcus luteusHarry R Beller
Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA 94608, USA
Appl Environ Microbiol 76:1212-23. 2010.... - Cloning of casbene and neocembrene synthases from Euphorbiaceae plants and expression in Saccharomyces cerevisiaeJames Kirby
California Institute for Quantitative Biosciences QB3, University of California, Berkeley, CA 94720, USA
Phytochemistry 71:1466-73. 2010..Casbene production levels of 31 mg/L were achieved in S. cerevisiae and we discuss strategies to further increase production by maximizing flux through the mevalonate pathway... - Expression of a synthetic Artemesia annua amorphadiene synthase in Aspergillus nidulans yields altered product distributionDavid Lubertozzi
Department of Chemical Engineering, University of California, Berkeley, CA 94720, USA
J Ind Microbiol Biotechnol 35:1191-8. 2008..In contrast, expression of ADS in Escherichia coli produced almost exclusively amorpha-4,11-diene. These results indicate that the host environment can greatly impact the terpenes produced from terpene synthases... - Enzyme immobilization via silaffin-mediated autoencapsulation in a biosilica supportWesley D Marner
Dept of Chemical Engineering, University of California, Berkeley, CA 94720, USA
Biotechnol Prog 25:417-23. 2009..Under certain circumstances, the enzymatically-active biosilica displayed improved stability relative to free enzyme at elevated temperatures... - Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuelsSung Kuk Lee
Joint BioEnergy Institute, Emeryville, CA 95608, USA
Curr Opin Biotechnol 19:556-63. 2008.... - Importance of systems biology in engineering microbes for biofuel productionAindrila Mukhopadhyay
Joint BioEnergy Institute, Emeryville, CA 95608, United States
Curr Opin Biotechnol 19:228-34. 2008.... - Microbial sensors for small molecules: development of a mevalonate biosensorBrian F Pfleger
Department of Chemical Engineering, University of California Berkeley, Berkeley, CA 94720 1462, USA
Metab Eng 9:30-8. 2007.... - Combinatorial engineering of intergenic regions in operons tunes expression of multiple genesBrian F Pfleger
Department of Chemical Engineering, University of California-Berkeley, Berkeley, CA 94720-1462, USA
Nat Biotechnol 24:1027-32. 2006..This technology should be useful for optimizing the expression of multiple genes in synthetic operons, both in prokaryotes and eukaryotes... - Metabolic engineering of a novel propionate-independent pathway for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in recombinant Salmonella enterica serovar typhimuriumIlana S Aldor
Department of Chemical Engineering, University of California, Berkeley, California 94720 1462, USA
Appl Environ Microbiol 68:3848-54. 2002..It was possible to vary the average HV fraction in the copolymer by adjusting the arabinose or cyanocobalamin (precursor of coenzyme B12) concentration in the medium... - Catabolite repression of the propionate catabolic genes in Escherichia coli and Salmonella enterica: evidence for involvement of the cyclic AMP receptor proteinSung Kuk Lee
Department of Chemical Engineering, University of California, Berkeley, CA 94720 1462, USA
J Bacteriol 187:2793-800. 2005.... - Propionate-regulated high-yield protein production in Escherichia coliSung Kuk Lee
Department of Chemical Engineering and Bioengineering, University of California, Synthetic Biology Department, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
Biotechnol Bioeng 93:912-8. 2006..In addition, propionate is an inexpensive, simple-to-use, nontoxic inducer that is attractive for large-scale protein production. Hence, this new system is highly complementary to the widely used T7 promoter-driven expression systems... - Amplification of HMG-CoA reductase production enhances carotenoid accumulation in Neurospora crassaGuang-Yi Wang
Department of Chemical Engineering, University of California at Berkeley, Berkeley, California 94720-1462, USA
Metab Eng 4:193-201. 2002..5-fold, respectively, relative to the wild-type strain. Over-expression of cHMG1 under control of the alcA promoter increased production of lycopene and neurosporaxanthin 3- and 2-fold, respectively... - Effect of gene location, mRNA secondary structures, and RNase sites on expression of two genes in an engineered operonChristina D Smolke
Department of Chemical Engineering, University of California, Berkeley 94720 1462, USA
Biotechnol Bioeng 80:762-76. 2002..By changing gene location and incorporating secondary structures and RNase E sites the relative steady-state transcript and protein levels encoded by the two reporter genes could be changed up to 100-fold and 750-fold, respectively... - Microbioreactor arrays with parametric control for high-throughput experimentationMichel M Maharbiz
Berkeley Sensor and Actuator Center (BSAC, Dept. of Electrical Engineering and Computer Science, University of California at Berkeley, Berkeley, California 94720, USA
Biotechnol Bioeng 86:485-90. 2004..Growth data are presented for Escherichia coli cultured in the array with varying microaerobic conditions using electrochemically generated oxygen. Additionally, we present data on carbon dioxide generation for pH dosing... - Improved assembly of multimeric genes for the biosynthetic production of protein polymersNichole L Goeden-Wood
Department of Chemical Engineering, University of California at Berkeley, Berkeley, California 94720-1462, USA
Biomacromolecules 3:874-9. 2002..Circular dichroism studies on the artificial protein poly-EAK9 demonstrate the formation of a beta-sheet structure in aqueous solution... - Application of functional genomics to pathway optimization for increased isoprenoid productionLance Kizer
Department of Chemical Engineering, University of California, Berkeley, California 94720 1462, USA
Appl Environ Microbiol 74:3229-41. 2008..This work demonstrates the utility of using transcriptomic and metabolomic methods to optimize synthetic biological systems... - Biosynthesis and engineering of isoprenoid small moleculesSydnor T Withers
Department of Chemical Engineering, University of California, Berkeley, CA, USA
Appl Microbiol Biotechnol 73:980-90. 2007..Finally, we will describe the efforts to produce to commercial terpenoids, paclitaxel and artemisinin... - Modulation of gene expression from the arabinose-inducible araBAD promoterA Khlebnikov
Department of Chemical Engineering, University of California, Berkeley, CA 94720-1462, USA
J Ind Microbiol Biotechnol 29:34-7. 2002.... - Effect of lacY expression on homogeneity of induction from the P(tac) and P(trc) promoters by natural and synthetic inducersArtem Khlebnikov
Department of Chemical Engineering, University of California, Berkeley, California 94720-1462, USA
Biotechnol Prog 18:672-4. 2002..In contrast, induction with IPTG gave rise to a single population of cells at all inducer concentrations in both lacY and lacY(+) strains... - A Salmonella-based, propionate-inducible, expression system for Salmonella entericaSung Kuk Lee
Department of Chemical Engineering, University of California, Berkeley, 94720, USA
Gene 377:6-11. 2006..In contrast, the prpB(-) strain showed very high background expression in both culture tubes and shake flasks... - Biosensors and their applications in microbial metabolic engineeringFuzhong Zhang
Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA 94608, USA
Trends Microbiol 19:323-9. 2011..This review will focus on recent developments in biosensor design, and their applications for controlling microbial behavior... - Designed divergent evolution of enzyme functionYasuo Yoshikuni
UCSF/UCB Joint Graduate Group in Bioengineering, Lawrence Berkeley National Laboratory, Berkeley, California 94710, USA
Nature 440:1078-82. 2006..Creation of these enzymes demonstrates the feasibility of exploiting the underlying evolvability of this scaffold, and provides evidence that rational approaches based on these ideas are useful for enzyme design... - Metabolic engineering for drug discovery and developmentChaitan Khosla
Department of Chemical Engineering, Stanford University, Stanford, California 94305, USA
Nat Rev Drug Discov 2:1019-25. 2003 - Advanced biofuel production in microbesPamela P Peralta-Yahya
Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA 94608, USA
Biotechnol J 5:147-62. 2010..We concentrate on the metabolic engineering of genetically tractable organisms such as Escherichia coli and Saccharomyces cerevisiae for the production of these advanced biofuels... - Engineering static and dynamic control of synthetic pathwaysWilliam J Holtz
Department of Electrical Engineering and Computer Science, University of California at Berkeley, Berkeley, CA 94720, USA
Cell 140:19-23. 2010..The next frontier is to create synthetic pathways that adapt to changing environments... - Microbioreactor arrays with parametric control for high-throughput experimentationMichel M Maharbiz
Berkeley Sensor and Actuator Center (BSAC, Dept. of Electrical Engineering and Computer Science, 497 Cory Hall, University of California at Berkeley, Berkeley, California 94720, USA
Biotechnol Bioeng 85:376-81. 2004..Growth data are presented for Escherichia coli cultured in the array with varying microaerobic conditions using electrochemically generated oxygen. Additionally, we present data on carbon dioxide generation for pH dosing... - Rationally engineered biotransformation of p-nitrophenolMatthew de la Peña Mattozzi
Dept of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA
Biotechnol Prog 26:616-21. 2010..Although P. putida harboring the refactored construct was incapable of growing exclusively on PNP as a carbon source, evidence that the engineered pathway was functional was demonstrated by the induced expression of chromosomal pcaIJF... - Chlorxanthomycin, a fluorescent, chlorinated, pentacyclic pyrene from a Bacillus spAndrew Magyarosy
Department of Chemical Engineering, University of California, Berkeley, California 94720-1462, USA
Appl Environ Microbiol 68:4095-101. 2002..7865. Chlorxanthomycin appears to be located in the cytoplasm, does not diffuse out of the cells into the culture medium, and has selective antibiotic activity... - Programming cellular functionChristopher A Voigt
Department of Pharmaceutical Chemistry and California Institute for Quantitative Biomedical Research QB3, University of California, San Francisco, San Francisco, California 94143 2540, USA
Nat Chem Biol 1:304-7. 2005..A meeting was held in August 2005 to present progress in the field and to discuss topics in ethics, safety and security... - Process design for microbial plastic factories: metabolic engineering of polyhydroxyalkanoatesIlana S Aldor
Department of Chemical Engineering, 201 Gilman Hall, University of California, Berkeley, CA 94720 1462, USA
Curr Opin Biotechnol 14:475-83. 2003..In addition, mathematical models and molecular methods can be used to elucidate metabolically engineered systems and to identify targets for performance improvement... - Manipulation of intracellular magnesium levels in Saccharomyces cerevisiae with deletion of magnesium transportersBernardo M T da Costa
Department of Chemical Engineering, University of California, Berkeley, CA 94720 3224, USA
Appl Microbiol Biotechnol 77:411-25. 2007..cerevisiae and overexpression of magnesium transporters from A. thaliana also affected the intracellular concentrations of a range of metal ions, which suggests that cells use non-specific transporters to help regulate metal homeostasis... - Design and construction of a double inversion recombination switch for heritable sequential genetic memoryTimothy S Ham
Department of Bioengineering, University of California, Berkeley, California, United States of America
PLoS ONE 3:e2815. 2008..Further, it is in theory possible to create complex sequential logics by careful positioning of recombinase recognition sites in the sequence... - Biofuel alternatives to ethanol: pumping the microbial wellJ L Fortman
Department of Chemical Engineering, University of California, 717 Potter Street, Building 977, Mail code 3224 Berkeley, CA 94720 3224, USA
Trends Biotechnol 26:375-81. 2008..To highlight additional options, we review advances in microbial engineering for the production of other potential fuel molecules, using a variety of biosynthetic pathways... - A tightly regulated inducible expression system utilizing the fim inversion recombination switchTimothy S Ham
Department of Bioengineering, University of California, Berkeley, 94720, USA
Biotechnol Bioeng 94:1-4. 2006..These characteristics make our system suitable for large-scale expression or for production of toxic proteins... - Absence of diauxie during simultaneous utilization of glucose and Xylose by Sulfolobus acidocaldariusChijioke J Joshua
Department of Plant and Microbial Biology, University of California, Berkeley, California, USA
J Bacteriol 193:1293-301. 2011..The mechanism by which the organism utilized a mixture of sugars has yet to be elucidated... - Engineering the lycopene synthetic pathway in E. coli by comparison of the carotenoid genes of Pantoea agglomerans and Pantoea ananatisSang Hwal Yoon
Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, 660 701, South Korea
Appl Microbiol Biotechnol 74:131-9. 2007..agglomerans and P. ananatis. As there was no significant difference in lycopene production between E. coli harboring P. agglomerans crtE and A. fulgidus gps, farnesyl diphosphate (FPP) synthesis was not rate-limiting in E. coli... - Increased beta-carotene production in recombinant Escherichia coli harboring an engineered isoprenoid precursor pathway with mevalonate additionSang Hwal Yoon
Division of Applied Life Science BK21, EB NCRC and PMBBRC, and Division of Forest Science, Gyeongsang National University, Jinju 660 701, Korea
Biotechnol Prog 23:599-605. 2007..5% (w/v) glycerol, beta-carotene production of 503 mg/L in concentration and 49.3 mg/g DCW in content was obtained at 144 h, which was the highest level of carotenoid production in E. coli ever reported in the literature... - Identification of genes affecting lycopene accumulation in Escherichia coli using a shot-gun methodMin Jung Kang
Amicogen Inc, Jinsung, Jinju 660 852, Korea
Biotechnol Bioeng 91:636-42. 2005..The co-expression of appY with dxs produced eight times the amount of lycopene (4.7 mg/g DCW) that was produced without expression of both genes (0.6 mg/g DCW)... - Charge-associated effects of fullerene derivatives on microbial structural integrity and central metabolismYinjie J Tang
Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
Nano Lett 7:754-60. 2007..The results presented here favor the hypothesis that fullerenes cause more membrane stress 4-6 than perturbation to energy metabolism.7... - Metabolic flux elucidation for large-scale models using 13C labeled isotopesPatrick F Suthers
Department of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802, USA
Metab Eng 9:387-405. 2007..Finally, we discuss the effect of reducing the model, as well as shed light onto the customization of the developed computational framework to other systems... - Separation and mass spectrometry in microbial metabolomicsDavid E Garcia
Joint BioEnergy Institute, Emeryville, CA 94608, USA
Curr Opin Microbiol 11:233-9. 2008.... - Cell-wide responses to low-oxygen exposure in Desulfovibrio vulgaris HildenboroughAindrila Mukhopadhyay
Berkeley Center for Synthetic Biology, 717 Potter Street, Berkeley, CA 94720, USA
J Bacteriol 189:5996-6010. 2007..vulgaris and suggest that while exposure to air is highly detrimental to D. vulgaris, this bacterium can successfully cope with periodic exposure to low O(2) levels in its environment... - Morphology of artificial silica matrices formed via autosilification of a silaffin/protein polymer chimeraWesley D Marner
Department of Chemical Engineering, University of California, Berkeley, California 94720, USA
Biomacromolecules 9:1-5. 2008.... - Microbially derived artemisinin: a biotechnology solution to the global problem of access to affordable antimalarial drugsVictoria Hale
The Institute for OneWorld Health, 50 California Street, San Francisco, CA 94111, USA
Am J Trop Med Hyg 77:198-202. 2007..By making life-saving ACTs affordable to the people who most need them, the Artemisinin Project hopes to show that the power of biotechnology can be harnessed to provide solutions to global health problems... - Redirection of flux through the FPP branch-point in Saccharomyces cerevisiae by down-regulating squalene synthaseEric M Paradise
Department of Chemical Engineering, University of California, Berkeley, California 94720, USA
Biotechnol Bioeng 100:371-8. 2008..The levels of these three metabolites were dependent not only upon the level of ERG9 repression, but also the timing of its repression relative to the induction of ADS and genes responsible for enhancing flux to FPP... - Isotopomer distributions in amino acids from a highly expressed protein as a proxy for those from total proteinAfshan S Shaikh
Department of Chemical Engineering, University of California, Berkeley, California 94720, USA
Anal Chem 80:886-90. 2008..This study provides the foundation to extend isotopomer-based flux analysis to study metabolism of individual strains in microbial communities... - Flux analysis of central metabolic pathways in Geobacter metallireducens during reduction of soluble Fe(III)-nitrilotriacetic acidYinjie J Tang
Synthetic Biology Department, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 3224, USA
Appl Environ Microbiol 73:3859-64. 2007..These findings enable a better understanding of the relationship between genome annotation and extant metabolic pathways in G. metallireducens... - Evaluation of the effects of various culture conditions on Cr(VI) reduction by Shewanella oneidensis MR-1 in a novel high-throughput mini-bioreactorYinjie J Tang
Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA
Biotechnol Bioeng 95:176-84. 2006..Besides its use for examining parameters of interest to environmental remediation, the device will also allow one to quickly assess parameters for optimal production of recombinant proteins or secondary metabolites... - Metabolic engineering of Pseudomonas putida for the utilization of parathion as a carbon and energy sourceAndy W Walker
Department of Chemical Engineering, University of California, Berkeley 94720-1462, USA
Biotechnol Bioeng 78:715-21. 2002..8 mM parathion as a source of carbon and energy. Degradation studies showed that parathion formed a separate dense, non-aqueous phase liquid phase but was still bioavailable... - Coenzyme Q10 production in recombinant Escherichia coli strains engineered with a heterologous decaprenyl diphosphate synthase gene and foreign mevalonate pathwayHossein Shahbani Zahiri
Department of Microbiology, Gyeongsang National University, Gazwa-dong 900, Jinju 660-701, Korea
Metab Eng 8:406-16. 2006..However, it provided CoQ(10) production of up to 1,706+/-86 microg/gDCW, which was roughly 1.9 times higher than that obtained by ddsA alone... - Uranyl precipitation by Pseudomonas aeruginosa via controlled polyphosphate metabolismNeil Renninger
Department of Chemical Engineering, University of California at Berkeley, Berkeley, CA 94720-1462, USA
Appl Environ Microbiol 70:7404-12. 2004..Lethal doses of radiation did not halt phosphate secretion from polyphosphate-filled cells under carbon starvation conditions... - Dibenzothiophene biodesulfurization pathway improvement using diagnostic GFP fusionsDavid S Reichmuth
Department of Chemical Engineering, University of California, Berkeley, CA 94720, USA
Biotechnol Bioeng 88:94-9. 2004..When cotransformed with the full dszABC operon, the mutant dszB increased the rate of desulfurization ninefold relative to that using the native dszB... - Post-translational modifications of Desulfovibrio vulgaris Hildenborough sulfate reduction pathway proteinsSara P Gaucher
Sandia National Laboratory, Livermore, California 94550, USA
J Proteome Res 7:2320-31. 2008..Here, we discuss our methods and implications of potential trimethylation in the D. vulgaris sulfate reduction pathway... - Engineering triterpene production in Saccharomyces cerevisiae-beta-amyrin synthase from Artemisia annuaJames Kirby
California Institute for Quantitative Biomedical Research, University of California, Berkeley, CA 94720, USA
FEBS J 275:1852-9. 2008..L(-1) culture. As we have observed a 12-fold increase in squalene levels, it appears that this strain has the capacity for even higher beta-amyrin production. Options for further engineering efforts are explored... - Metabolic engineering delivers next-generation biofuelsJay D Keasling
Nat Biotechnol 26:298-9. 2008 - Enhanced lycopene production in Escherichia coli engineered to synthesize isopentenyl diphosphate and dimethylallyl diphosphate from mevalonateSang-Hwal Yoon
Department of Food Science and Nutrition, Gyeongsang National University, Jinju, Korea
Biotechnol Bioeng 94:1025-32. 2006..6 mM mevalonate for the mevalonate lower pathway, and 0.5% (w/v) Tween 80 to prevent clump formation, lycopene production was 102 mg/L and 22 mg/g dry cell weight, and cell growth had an OD(600) value of 15 for 72 h...
Research Grants
- Biosensors for metabolic engineeringJay Keasling; Fiscal Year: 2006..abstract_text> ..