Research Topics
Genomes and GenesSpecies | B K KaySummaryAffiliation: University of Wisconsin Country: USA Publications
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Publications
Identification of a novel domain shared by putative components of the endocytic and cytoskeletal machineryB K Kay
Department of Pharmacology, University of Wisconsin Madison, 53706 1532, USA
Protein Sci 8:435-8. 1999..We have named this protein segment the ENTH domain, for Epsin N-terminal Homology domain, and hypothesize that it is a candidate for binding specific ligands and/or enzymatic activity in the cell...
Identification of enzyme inhibitors from phage-displayed combinatorial peptide librariesB K Kay
Department of Pharmacology, University of Wisconsin Madison, 1300 University Avenue, Madison, WI 53706 1532, USA
Comb Chem High Throughput Screen 4:535-43. 2001..e., as a lead compound for peptidomimetic design or as displaceable probe in high-throughput screens of libraries of small molecules)...- Screening phage-displayed combinatorial peptide librariesB K Kay
Department of Pharmacology, University of Wisconsin Madison, 1300 University Avenue, Madison, Wisconsin 53706 1532, USA
Methods 24:240-6. 2001..In this article, protocols and examples are provided to guide scientists who wish to identify peptide ligands to their favorite proteins... - Convergent evolution with combinatorial peptidesB K Kay
Department of Pharmacology, University of Wisconsin Madison, WI 53706 1532, USA
FEBS Lett 480:55-62. 2000..Thus, a fruitful approach for mapping protein-protein interactions is to isolate peptide ligands to a target protein and identify candidate interacting proteins in a sequenced genome by computer analysis... - The importance of being proline: the interaction of proline-rich motifs in signaling proteins with their cognate domainsB K Kay
Department of Pharmacology, University of Wisconsin Madison, Madison, Wisconsin 53706 1532, USA
FASEB J 14:231-41. 2000..Kay, B. K., Williamson, M. P., Sudol, M. The importance of being proline: the interaction of proline-rich motifs in signaling proteins with their cognate domains... - Intersectin, a novel adaptor protein with two Eps15 homology and five Src homology 3 domainsM Yamabhai
Department of Pharmacology, University of Wisconsin, Madison, Wisconsin 53706 1532, USA
J Biol Chem 273:31401-7. 1998..We propose that Intersectin is a component of the endocytic machinery... - Molecular mechanism of NPF recognition by EH domainsT de Beer
Department of Pharmacology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, Colorado 80262, USA
Nat Struct Biol 7:1018-22. 2000.... - Physical and functional interactions between the transactivation domain of the hematopoietic transcription factor NF-E2 and WW domainsE A Mosser
Department of Pharmacology, University of Wisconsin Medical School, Madison 53706, USA
Biochemistry 37:13686-95. 1998..Mutation of PPXY-1, but not PPXY-2, inhibited the transactivation function of NF-E2, providing support for the hypothesis that WW domain interactions are important for NF-E2-mediated transactivation... - Splice variants of intersectin are components of the endocytic machinery in neurons and nonneuronal cellsN K Hussain
Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC, H3A 2B4, Canada
J Biol Chem 274:15671-7. 1999..These data suggest that the long and short forms of intersectin are components of the endocytic machinery in neurons and nonneuronal cells... - Endocytic protein intersectin-l regulates actin assembly via Cdc42 and N-WASPN K Hussain
Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec, H3A 2B4, Canada
Nat Cell Biol 3:927-32. 2001..These studies reveal a role for intersectin-l in a novel mechanism of N-WASP activation and in regulation of the actin cytoskeleton... - Cloning of ligand targets: systematic isolation of SH3 domain-containing proteinsA B Sparks
Department of Biology, University of North Carolina, Chapel Hill 27599, USA
Nat Biotechnol 14:741-4. 1996..The isolation of entire repertoires of modular domain-containing proteins will prove invaluable in genome analysis and in bringing new targets into drug discovery programs... - A role for CAP, a novel, multifunctional Src homology 3 domain-containing protein in formation of actin stress fibers and focal adhesionsV Ribon
Department of Physiology, University of Michigan School of Medicine, Ann Arbor, Michigan 48109, USA
J Biol Chem 273:4073-80. 1998..These results suggest that CAP may mediate signals for the formation of stress fibers and focal adhesions... - The endocytic protein intersectin is a major binding partner for the Ras exchange factor mSos1 in rat brainX K Tong
Department of Neurology, Montreal Neurological Institute, Montreal, QC H3A 2B4, Canada
EMBO J 19:1263-71. 2000..These results suggest that intersectin functions in cell signaling in addition to its role in endocytosis and may link these cellular processes... - A novel, multifuntional c-Cbl binding protein in insulin receptor signaling in 3T3-L1 adipocytesV Ribon
Department of Physiology, University of Michigan School of Medicine, Ann Arbor 48109, USA
Mol Cell Biol 18:872-9. 1998..Insulin stimulation resulted in the dissociation of CAP from the insulin receptor. Taken together, these data suggest that CAP represents a novel c-Cbl binding protein in 3T3-L1 adipocytes likely to participate in insulin signaling... - Synaptojanin forms two separate complexes in the nerve terminal. Interactions with endophilin and amphiphysinK D Micheva
Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, 3801 rue University, Montreal, Quebec H3A 2B4, Canada
J Biol Chem 272:27239-45. 1997..These data demonstrate that synaptojanin is present in two separate complexes in the nerve terminal and support an important role for endophilin in the regulation of synaptojanin function... - Characterizing Class I WW domains defines key specificity determinants and generates mutant domains with novel specificitiesJ Kasanov
Department of Biology, University of North Carolina, Chapel Hill 27599-3280, USA
Chem Biol 8:231-41. 2001..The presence of these residues within an uncharacterized WW domain can be used to predict its ability to bind Pro-Xxx-Tyr containing peptide ligands... - SH3 domain-dependent interactions of endophilin with amphiphysinK D Micheva
Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Que, Canada
FEBS Lett 414:308-12. 1997..The interaction is direct and mediated through a 43 amino acid region of amphiphysin containing the putative SH3 binding site. These data further support a role for amphiphysin I, II and endophilin in synaptic vesicle endocytosis... - Isolation of peptides from phage-displayed random peptide libraries that interact with the talin-binding domain of vinculinN B Adey
Department of Biology, University of North Carolina, Chapel Hill, NC 27599 3280, USA
Biochem J 324:523-8. 1997..This study demonstrates that the vinculin-binding peptides interact with vinculin in a biologically relevant manner and represent an excellent tool for further study of the biochemistry of vinculin... - Potential for two isoforms of the A1 ribonucleoprotein in Xenopus laevisB K Kay
Department of Biology, University of North Carolina, Chapel Hill 27599 3280
Proc Natl Acad Sci U S A 87:1367-71. 1990..Our findings suggest that the isoforms are encoded by one or two genes and are the result of alternative splicing. We discuss the biological implications of having two forms of the A1 component of hnRNP particles...