Research Topics
Genomes and Genes | David G KaufmanSummaryAffiliation: University of North Carolina Country: USA Publications
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Publications
Same origins of DNA replication function on the active and inactive human X chromosomesStephanie M Cohen
Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7525, USA
J Cell Biochem 88:923-31. 2003..Therefore, transcription activity at the HPRT and G6PD genes is not necessary for initiation of DNA replication at the origins mapped to these chromosomal loci...
Temporal and functional analysis of DNA replicated in early S phaseDavid G Kaufman
Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
Adv Enzyme Regul 51:257-71. 2011..Such studies could prove very valuable in studies of the mechanisms of cancer development, aging, and other processes of disordered genomic functioning...- Mapping of an origin of DNA replication in the promoter of fragile X gene FMR1Bruna P Brylawski
Department of Pathology and Laboratory Medicine, C B 7525, University of North Carolina, Chapel Hill, NC 27599 7525, USA
Exp Mol Pathol 82:190-6. 2007..The position of the origin of replication relative to the CGG repeat, and perhaps the late replication of these genes, might be important factors in the susceptibility to triplet repeat amplification at the FRAXA and FRAXE sites... - Mapping of an origin of DNA replication near the transcriptional promoter of the human HPRT geneStephanie M Cohen
Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7525, USA
J Cell Biochem 85:346-56. 2002..5 kb upstream of a previously reported autonomously replicating sequence (Sykes et al. [1988] Mol. Gen. Genet. 212:301-309)... - A late origin of DNA replication in the trinucleotide repeat region of the human FMR2 genePaul D Chastain
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina 27599 7525, USA
Cell Cycle 5:869-72. 2006.... - Transitions in replication timing in a 340 kb region of human chromosomal R-Band 1p36.1Bruna P Brylawski
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina 27599-7525, USA
J Cell Biochem 92:755-69. 2004.... - DNA replication in early S phase pauses near newly activated originsRebecca A Frum
Department of Pathology and Laboratory Medicine, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7525, USA
Cell Cycle 7:1440-8. 2008..Further, it is possible that the loss of this regulatory process in cancer cells such as T98G could be a contributing factor in the genetic instability that typifies cancers... - BRG1 co-localizes with DNA replication factors and is required for efficient replication fork progressionStephanie M Cohen
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC 27599, USA
Nucleic Acids Res 38:6906-19. 2010..This novel function of BRG1 is consistent with its requirement during embryogenesis and its role as a tumor suppressor to maintain genome stability and prevent cancer... - Early S phase DNA replication: a search for targets of carcinogenesisDavid G Kaufman
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC 27599-7525, USA
Adv Enzyme Regul 47:127-38. 2007 - Temporal differences in DNA replication during the S phase using single fiber analysis of normal human fibroblasts and glioblastoma T98G cellsRebecca A Frum
Department of Pathology and Laboratory Medicine, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
Cell Cycle 8:3133-48. 2009..Our observations suggest models to explain the temporal replication of single and clustered origins, and suggest differences in the replication program in a normal and cancer cell line... - Complementation of replication origin function in mouse embryonic stem cells by human DNA sequencesStephanie M Cohen
Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7525, USA
Genomics 84:475-84. 2004..The genetic information contained in the human sequence and surrounding mouse DNA was analyzed for cis-acting elements that might contribute to selection and functional activation of a mammalian origin of DNA replication... - Accumulation of true single strand breaks and AP sites in base excision repair deficient cellsApril M Luke
Curriculum in Toxicology, University of North Carolina, Chapel Hill, USA
Mutat Res 694:65-71. 2010..Furthermore, the Pol β-null cells displayed greater AP site formation than the parental DT40 cells. OTX use represents a facile approach for assessing SSB formation, whose benefits can also be applied to other established SSB assays... - Checkpoint regulation of replication dynamics in UV-irradiated human cellsPaul D Chastain
Department of Pathology and Laboratory Medicine, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7525 USA
Cell Cycle 5:2160-7. 2006..These findings illustrate the concordance of data derived from different experimental approaches, thus strengthening the evidence that the activation of the intra-S checkpoint by UVC is dependent on the ATR and Chk1 kinases... - Genome-wide sequence and functional analysis of early replicating DNA in normal human fibroblastsStephanie M Cohen
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina 27599, USA
BMC Genomics 7:301. 2006..This was accomplished by first creating a cosmid library containing DNA enriched in sequences that replicate early in the S phase of normal human fibroblasts. Clone ends were then sequenced and aligned to the human genome... - Early replication and the apoptotic pathwayStephanie M Cohen
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina 27599 7525, USA
J Cell Physiol 213:434-9. 2007..This finding leads us to question how and why the replication of genes in the apoptotic pathway is temporally organized in this manner. Here we discuss the possible explanations and implications of this observation... - Genistein effects on stromal cells determines epithelial proliferation in endometrial co-culturesBrante P Sampey
Department of Pathology and Laboratory Medicine, 620 Brinkhous Bullitt Building, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7525, USA
Exp Mol Pathol 90:257-63. 2011..However, results from previous studies are conflicting regarding the effects of genistein on hormone responsive cancers... - DNA replication and the GINS complex: localization on extended chromatin fibersStephanie M Cohen
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, USA
Epigenetics Chromatin 2:6. 2009..Additionally, the large size of our chromatin fibers (up to approximately 7 Mb) allowed for a more expansive analysis of the distance between active replicons than previously reported... - Abasic sites preferentially form at regions undergoing DNA replicationPaul D Chastain
Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7525, USA
FASEB J 24:3674-80. 2010..They also reveal that there is increased susceptibility to oxidative damage in DNA regions undergoing replication, which may explain the previously observed clustering of AP sites... - Effects of tibolone metabolites on human endometrial cell lines in co-cultureClaire Barbier
Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, NC 27599 7525, USA
Reprod Sci 15:75-82. 2008..Thus, the unexpected progestagenic effect of 3beta-OH-tibolone in these co-cultures may be due to metabolic activity present in the stromal cells of the co-cultures... - Expression of exogenous human telomerase in cultures of endometrial stromal cells does not alter their hormone responsivenessClaire S Barbier
Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, 27599, USA
Biol Reprod 73:106-14. 2005....
Research Grants
- CANCER SUSCEPTIBILITY AND S PHASE INITIATION SITESDavid Kaufman; Fiscal Year: 2009..Specific Aim 3: Analyze the architecture of replication and potential fork progression barriers in an early replicated region of the human genome. ..
- Estrogens, Paracrine Factors, and Endometrial CancerDavid Kaufman; Fiscal Year: 2006..This study will test whether the hypothesized mechanism explains the differing responses of endometrium to estrogens and may allow development of a better assay of effects of SERMs and phytoestrogens on endometrium. ..
- Identification of Areas of Oxidative Damage in Human Genomic DNADavid Kaufman; Fiscal Year: 2007..Finally, we will determine whether it is possible to detect visual signals from all three techniques (AP sites, FISH, DNA counterstain) in the same experimental samples. ASSESSMENT: ..
- MINORITY UNDERGRADUATE RESEARCH IN ENVIRONMENTAL HEALTHDavid Kaufman; Fiscal Year: 2007..They believe that they have made very good progress toward this goal during the first ten years of this Program. ..
- ENVIRONMENTAL PATHOLOGYDavid Kaufman; Fiscal Year: 2007..Faculty members also participate on various risk assessment panels. Excellent facilities, outstanding faculty and research projects characterize this Training Program. ..
- Transformation of Human Endometrial Epithelial CellsDavid Kaufman; Fiscal Year: 2005..abstract_text> ..
- EARLY S PHASE REPLICATION SITES AS ENVIRONMENTAL TARGETSDavid Kaufman; Fiscal Year: 2002....
- CONFOCAL LASER SCANNING MICROSCOPEDavid Kaufman; Fiscal Year: 2003..Substantial preliminary and published data have been added to project descriptions. ..
- CANCER SUSCEPTIBILITY AND S PHASE INITIATION SITESDavid Kaufman; Fiscal Year: 2003..These alterations may be another type of genetic change that initiates the process of carcinogenesis. ..