Research Topics
| George A KassavetisSummaryAffiliation: University of California Country: USA Publications
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Detail Information
Publications
Effects of DNA strand breaks on transcription by RNA polymerase III: insights into the role of TFIIIB and the polarity of promoter openingGeorge A Kassavetis
Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA or
EMBO J 21:5508-15. 2002....- The RNA polymerase III transcription initiation factor TFIIIB participates in two steps of promoter openingG A Kassavetis
Department of Biology and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
EMBO J 20:2823-34. 2001..Removing an N-terminal domain of Brf interferes with downstream propagation of the transcription bubble to and beyond the transcriptional start site... - A minimal RNA polymerase III transcription systemG A Kassavetis
Department of Biology, Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
EMBO J 18:5042-51. 1999.... - Functional and structural organization of Brf, the TFIIB-related component of the RNA polymerase III transcription initiation complexG A Kassavetis
Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
Mol Cell Biol 18:5587-99. 1998..The constraints established by these analyses specify a global model of the functional segments of Brf and how they fit into the structure of the TFIIIB-DNA complex... 
Transcription factor TFIIIB and transcription by RNA polymerase IIIG A Kassavetis
Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
Biochem Soc Trans 34:1082-7. 2006....- The C53/C37 subcomplex of RNA polymerase III lies near the active site and participates in promoter openingGeorge A Kassavetis
Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093 0634, USA
J Biol Chem 285:2695-706. 2010.... - Nhp6 is a transcriptional initiation fidelity factor for RNA polymerase III transcription in vitro and in vivoGeorge A Kassavetis
Division of Biological Sciences and Center for Molecular Genetics, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
J Biol Chem 281:7445-51. 2006..Analyses of unprocessed tRNAs from yeast lacking Nhp6a and its closely related paralogue Nhp6b demonstrate that Nhp6 is required for transcriptional initiation fidelity of some but not all tRNA genes, in vivo... - Mapping the principal interaction site of the Brf1 and Bdp1 subunits of Saccharomyces cerevisiae TFIIIBGeorge A Kassavetis
Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
J Biol Chem 281:14321-9. 2006.... - Cloning, expression, and function of TFC5, the gene encoding the B" component of the Saccharomyces cerevisiae RNA polymerase III transcription factor TFIIIBG A Kassavetis
Department of Biology, University of California at San Diego, La Jolla 92093 0634, USA
Proc Natl Acad Sci U S A 92:9786-90. 1995..A truncated form of B" lacking 39 N-terminal and 107 C-terminal amino acids is also functional for transcription... - A common site on TBP for transcription by RNA polymerases II and IIIOliver Schroder
Division of Biological Sciences, Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
EMBO J 22:5115-24. 2003.... - A gene-specific effect of an internal deletion in the Bdp1 subunit of the RNA polymerase III transcription initiation factor TFIIIBAkira Ishiguro
Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
FEBS Lett 548:33-6. 2003..It is shown that TFIIIC-dependent assembly of TFIIIB on the RPR1 promoter is specifically sensitive to this Bdp1 deletion, leading to gene-specifically defective single-round and multiple-round transcription... - Essential roles of Bdp1, a subunit of RNA polymerase III initiation factor TFIIIB, in transcription and tRNA processingAkira Ishiguro
Division of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
Mol Cell Biol 22:3264-75. 2002..Physical interaction of RNase P with Bdp1 was demonstrated by coimmunoprecipitation and pull-down assays... - Bending of the Saccharomyces cerevisiae 5S rRNA gene in transcription factor complexesB R Braun
Department of Biology, University of California, San Diego, La Jolla 92093 0634
J Biol Chem 267:22562-9. 1992..Fully assembled transcription factor complexes bend the 5S rRNA gene in the same net direction as does TFIIIB alone... - Absolute gene occupancies by RNA polymerase III, TFIIIB, and TFIIIC in Saccharomyces cerevisiaeElisabetta Soragni
Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
J Biol Chem 283:26568-76. 2008.... - The role of transcription initiation factor IIIB subunits in promoter opening probed by photochemical cross-linkingGeorge A Kassavetis
Division of Biological Sciences and the Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
J Biol Chem 278:17912-7. 2003..In contrast, promoter opening-defective TFIIIB mutants generate significant changes of cross-linking of polymerase subunits. The weight of this evidence argues in favor of an indirect mode of action of TFIIIB in promoter opening... - The role of the TATA-binding protein in the assembly and function of the multisubunit yeast RNA polymerase III transcription factor, TFIIIBG A Kassavetis
Department of Biology, University of California, San Diego, La Jolla 92093 0634
Cell 71:1055-64. 1992..Incorporation of the 90 kd B" protein into the transcription complex requires TBP. The heparin-resistant TFIIIB-DNA complex retains all three of its constituent proteins, TBP, BRF, and B"... - Transcription: adjusting to adversity by regulating RNA polymeraseE Peter Geiduschek
Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093 0634, USA
Curr Biol 16:R849-51. 2006.... - Reconfiguring the connectivity of a multiprotein complex: fusions of yeast TATA-binding protein with Brf1, and the function of transcription factor IIIBGeorge A Kassavetis
Division of Biological Sciences, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
Proc Natl Acad Sci U S A 102:15406-11. 2005..The Brf1-TBP triple fusion protein also effectively replaces Brf1 function in vivo as the intact protein, creating a TBP paralogue in yeast that is privatized for pol III transcription... - Marking the start site of RNA polymerase III transcription: the role of constraint, compaction and continuity of the transcribed DNA strandAnne Grove
Division of Biology and Center for Molecular Genetics, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
EMBO J 21:704-14. 2002..Breaks in the non-transcribed strand generate only minor effects on transcription. A structure-based model interprets these observations and explains how the transcribed strand is used to locate the transcriptional start site... - Mutational and functional analysis of a segment of the sigma family bacteriophage T4 late promoter recognition protein gp55Kevin Wong
Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
J Biol Chem 278:7073-80. 2003..2 and the recently determined structures of the Thermus thermophilus and Thermus aquaticus sigma(70)-RNA polymerase holoenzymes... - Human TFIID binds to core promoter DNA in a reorganized structural stateMichael A Cianfrocco
Biophysics Graduate Group, University of California, Berkeley, Berkeley, CA 94720, USA
Cell 152:120-31. 2013..The existence of two structurally and functionally distinct forms of TFIID suggests that the different conformers may serve as specific targets for the action of regulatory factors... - A single-stranded promoter for RNA polymerase IIIOliver Schroder
Division of Biological Sciences and Center for Molecular Genetics, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0634, USA
Proc Natl Acad Sci U S A 100:934-9. 2003.... - The RNA polymerase III-recruiting factor TFIIIB induces a DNA bend between the TATA box and the transcriptional start siteA Grove
Department of Biology and Center for Molecular Genetics, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093 0634, USA
J Mol Biol 285:1429-40. 1999..We conclude that TFIIIB induces an additional DNA deformation between the TATA box and the start site of transcription that is likely to be more extended than the sharp kinks generated by TBP... - Multiple states of protein-DNA interaction in the assembly of transcription complexes on Saccharomyces cerevisiae 5S ribosomal RNA genesB R Braun
Department of Biology, University of California, San Diego, La Jolla 92093
Proc Natl Acad Sci U S A 86:2530-4. 1989..Complexes assembled from the complete set of purified transcription factors or from a complete transcription system extend over the entire transcription unit together with almost 50 base pairs of 5' flanking sequence... - Alignment of the B" subunit of RNA polymerase III transcription factor IIIB in its promoter complexS M Shah
Department of Biology, Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
J Biol Chem 274:28736-44. 1999..Examination of two-cross-link products formed by DNA with adjacent and nearby photoactive nucleotides supports the conclusion that Brf and B" share an extended interface along the length of the TFIIIB-DNA complex... - Domains of the Brf component of RNA polymerase III transcription factor IIIB (TFIIIB): functions in assembly of TFIIIB-DNA complexes and recruitment of RNA polymerase to the promoterG A Kassavetis
Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla 92093 0634, USA
Mol Cell Biol 17:5299-306. 1997..These findings suggest a functional complementarity and reciprocity between the Brf and B" components of TFIIIB... - Transcription of the T4 late genesE Peter Geiduschek
Division of Biological Sciences, Section of Molecular Biology, University of California, San Diego, La Jolla, CA 92093 0634, USA
Virol J 7:288. 2010..The emphasis of this account is on the sites and mechanisms of actions of these three proteins, and on their roles in the formation of transcription-ready open T4 late promoter complexes... - Molecular genetic analysis of a prokaryotic transcriptional coactivator: functional domains of the bacteriophage T4 gene 33 proteinJ W Winkelman
Department of Biology, University of California, San Diego, La Jolla 92093 0634
J Bacteriol 176:1164-71. 1994..The possible roles of gp33 in mediating late gene expression are discussed in the light of the identification of these functional domains... - Abortive initiation by Saccharomyces cerevisiae RNA polymerase IIIP Bhargava
Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
J Biol Chem 274:26550-6. 1999..This cleavage also occurs in the normal process of forming a productive elongation complex. The possible role of nucleolytic retraction in disengaging pol III from TFIIIB is discussed...