George A Kassavetis

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc A minimal RNA polymerase III transcription system
    G A Kassavetis
    Department of Biology, Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
    EMBO J 18:5042-51. 1999
  2. pmc Effects of DNA strand breaks on transcription by RNA polymerase III: insights into the role of TFIIIB and the polarity of promoter opening
    George A Kassavetis
    Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA or
    EMBO J 21:5508-15. 2002
  3. pmc Functional and structural organization of Brf, the TFIIB-related component of the RNA polymerase III transcription initiation complex
    G A Kassavetis
    Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
    Mol Cell Biol 18:5587-99. 1998
  4. ncbi request reprint Transcription factor TFIIIB and transcription by RNA polymerase III
    G A Kassavetis
    Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
    Biochem Soc Trans 34:1082-7. 2006
  5. pmc The C53/C37 subcomplex of RNA polymerase III lies near the active site and participates in promoter opening
    George A Kassavetis
    Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093 0634, USA
    J Biol Chem 285:2695-706. 2010
  6. pmc The RNA polymerase III transcription initiation factor TFIIIB participates in two steps of promoter opening
    G A Kassavetis
    Department of Biology and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
    EMBO J 20:2823-34. 2001
  7. ncbi request reprint Nhp6 is a transcriptional initiation fidelity factor for RNA polymerase III transcription in vitro and in vivo
    George A Kassavetis
    Division of Biological Sciences and Center for Molecular Genetics, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
    J Biol Chem 281:7445-51. 2006
  8. ncbi request reprint Mapping the principal interaction site of the Brf1 and Bdp1 subunits of Saccharomyces cerevisiae TFIIIB
    George A Kassavetis
    Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
    J Biol Chem 281:14321-9. 2006
  9. pmc Cloning, expression, and function of TFC5, the gene encoding the B" component of the Saccharomyces cerevisiae RNA polymerase III transcription factor TFIIIB
    G A Kassavetis
    Department of Biology, University of California at San Diego, La Jolla 92093 0634, USA
    Proc Natl Acad Sci U S A 92:9786-90. 1995
  10. pmc A common site on TBP for transcription by RNA polymerases II and III
    Oliver Schroder
    Division of Biological Sciences, Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
    EMBO J 22:5115-24. 2003

Collaborators

Detail Information

Publications30

  1. pmc A minimal RNA polymerase III transcription system
    G A Kassavetis
    Department of Biology, Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
    EMBO J 18:5042-51. 1999
    ....
  2. pmc Effects of DNA strand breaks on transcription by RNA polymerase III: insights into the role of TFIIIB and the polarity of promoter opening
    George A Kassavetis
    Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA or
    EMBO J 21:5508-15. 2002
    ....
  3. pmc Functional and structural organization of Brf, the TFIIB-related component of the RNA polymerase III transcription initiation complex
    G A Kassavetis
    Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
    Mol Cell Biol 18:5587-99. 1998
    ..The constraints established by these analyses specify a global model of the functional segments of Brf and how they fit into the structure of the TFIIIB-DNA complex...
  4. ncbi request reprint Transcription factor TFIIIB and transcription by RNA polymerase III
    G A Kassavetis
    Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
    Biochem Soc Trans 34:1082-7. 2006
    ....
  5. pmc The C53/C37 subcomplex of RNA polymerase III lies near the active site and participates in promoter opening
    George A Kassavetis
    Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093 0634, USA
    J Biol Chem 285:2695-706. 2010
    ....
  6. pmc The RNA polymerase III transcription initiation factor TFIIIB participates in two steps of promoter opening
    G A Kassavetis
    Department of Biology and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
    EMBO J 20:2823-34. 2001
    ..Removing an N-terminal domain of Brf interferes with downstream propagation of the transcription bubble to and beyond the transcriptional start site...
  7. ncbi request reprint Nhp6 is a transcriptional initiation fidelity factor for RNA polymerase III transcription in vitro and in vivo
    George A Kassavetis
    Division of Biological Sciences and Center for Molecular Genetics, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
    J Biol Chem 281:7445-51. 2006
    ..Analyses of unprocessed tRNAs from yeast lacking Nhp6a and its closely related paralogue Nhp6b demonstrate that Nhp6 is required for transcriptional initiation fidelity of some but not all tRNA genes, in vivo...
  8. ncbi request reprint Mapping the principal interaction site of the Brf1 and Bdp1 subunits of Saccharomyces cerevisiae TFIIIB
    George A Kassavetis
    Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
    J Biol Chem 281:14321-9. 2006
    ....
  9. pmc Cloning, expression, and function of TFC5, the gene encoding the B" component of the Saccharomyces cerevisiae RNA polymerase III transcription factor TFIIIB
    G A Kassavetis
    Department of Biology, University of California at San Diego, La Jolla 92093 0634, USA
    Proc Natl Acad Sci U S A 92:9786-90. 1995
    ..A truncated form of B" lacking 39 N-terminal and 107 C-terminal amino acids is also functional for transcription...
  10. pmc A common site on TBP for transcription by RNA polymerases II and III
    Oliver Schroder
    Division of Biological Sciences, Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
    EMBO J 22:5115-24. 2003
    ....
  11. ncbi request reprint A gene-specific effect of an internal deletion in the Bdp1 subunit of the RNA polymerase III transcription initiation factor TFIIIB
    Akira Ishiguro
    Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
    FEBS Lett 548:33-6. 2003
    ..It is shown that TFIIIC-dependent assembly of TFIIIB on the RPR1 promoter is specifically sensitive to this Bdp1 deletion, leading to gene-specifically defective single-round and multiple-round transcription...
  12. pmc Essential roles of Bdp1, a subunit of RNA polymerase III initiation factor TFIIIB, in transcription and tRNA processing
    Akira Ishiguro
    Division of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
    Mol Cell Biol 22:3264-75. 2002
    ..Physical interaction of RNase P with Bdp1 was demonstrated by coimmunoprecipitation and pull-down assays...
  13. ncbi request reprint Bending of the Saccharomyces cerevisiae 5S rRNA gene in transcription factor complexes
    B R Braun
    Department of Biology, University of California, San Diego, La Jolla 92093 0634
    J Biol Chem 267:22562-9. 1992
    ..Fully assembled transcription factor complexes bend the 5S rRNA gene in the same net direction as does TFIIIB alone...
  14. pmc Absolute gene occupancies by RNA polymerase III, TFIIIB, and TFIIIC in Saccharomyces cerevisiae
    Elisabetta Soragni
    Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
    J Biol Chem 283:26568-76. 2008
    ....
  15. ncbi request reprint The role of transcription initiation factor IIIB subunits in promoter opening probed by photochemical cross-linking
    George A Kassavetis
    Division of Biological Sciences and the Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
    J Biol Chem 278:17912-7. 2003
    ..In contrast, promoter opening-defective TFIIIB mutants generate significant changes of cross-linking of polymerase subunits. The weight of this evidence argues in favor of an indirect mode of action of TFIIIB in promoter opening...
  16. ncbi request reprint The role of the TATA-binding protein in the assembly and function of the multisubunit yeast RNA polymerase III transcription factor, TFIIIB
    G A Kassavetis
    Department of Biology, University of California, San Diego, La Jolla 92093 0634
    Cell 71:1055-64. 1992
    ..Incorporation of the 90 kd B" protein into the transcription complex requires TBP. The heparin-resistant TFIIIB-DNA complex retains all three of its constituent proteins, TBP, BRF, and B"...
  17. pmc Reconfiguring the connectivity of a multiprotein complex: fusions of yeast TATA-binding protein with Brf1, and the function of transcription factor IIIB
    George A Kassavetis
    Division of Biological Sciences, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
    Proc Natl Acad Sci U S A 102:15406-11. 2005
    ..The Brf1-TBP triple fusion protein also effectively replaces Brf1 function in vivo as the intact protein, creating a TBP paralogue in yeast that is privatized for pol III transcription...
  18. ncbi request reprint Transcription: adjusting to adversity by regulating RNA polymerase
    E Peter Geiduschek
    Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093 0634, USA
    Curr Biol 16:R849-51. 2006
    ....
  19. ncbi request reprint Mutational and functional analysis of a segment of the sigma family bacteriophage T4 late promoter recognition protein gp55
    Kevin Wong
    Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
    J Biol Chem 278:7073-80. 2003
    ..2 and the recently determined structures of the Thermus thermophilus and Thermus aquaticus sigma(70)-RNA polymerase holoenzymes...
  20. pmc Marking the start site of RNA polymerase III transcription: the role of constraint, compaction and continuity of the transcribed DNA strand
    Anne Grove
    Division of Biology and Center for Molecular Genetics, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
    EMBO J 21:704-14. 2002
    ..Breaks in the non-transcribed strand generate only minor effects on transcription. A structure-based model interprets these observations and explains how the transcribed strand is used to locate the transcriptional start site...
  21. pmc Human TFIID binds to core promoter DNA in a reorganized structural state
    Michael A Cianfrocco
    Biophysics Graduate Group, University of California, Berkeley, Berkeley, CA 94720, USA
    Cell 152:120-31. 2013
    ..The existence of two structurally and functionally distinct forms of TFIID suggests that the different conformers may serve as specific targets for the action of regulatory factors...
  22. pmc A single-stranded promoter for RNA polymerase III
    Oliver Schroder
    Division of Biological Sciences and Center for Molecular Genetics, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
    Proc Natl Acad Sci U S A 100:934-9. 2003
    ....
  23. pmc Multiple states of protein-DNA interaction in the assembly of transcription complexes on Saccharomyces cerevisiae 5S ribosomal RNA genes
    B R Braun
    Department of Biology, University of California, San Diego, La Jolla 92093
    Proc Natl Acad Sci U S A 86:2530-4. 1989
    ..Complexes assembled from the complete set of purified transcription factors or from a complete transcription system extend over the entire transcription unit together with almost 50 base pairs of 5' flanking sequence...
  24. pmc Domains of the Brf component of RNA polymerase III transcription factor IIIB (TFIIIB): functions in assembly of TFIIIB-DNA complexes and recruitment of RNA polymerase to the promoter
    G A Kassavetis
    Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla 92093 0634, USA
    Mol Cell Biol 17:5299-306. 1997
    ..These findings suggest a functional complementarity and reciprocity between the Brf and B" components of TFIIIB...
  25. ncbi request reprint The RNA polymerase III-recruiting factor TFIIIB induces a DNA bend between the TATA box and the transcriptional start site
    A Grove
    Department of Biology and Center for Molecular Genetics, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093 0634, USA
    J Mol Biol 285:1429-40. 1999
    ..We conclude that TFIIIB induces an additional DNA deformation between the TATA box and the start site of transcription that is likely to be more extended than the sharp kinks generated by TBP...
  26. ncbi request reprint Alignment of the B" subunit of RNA polymerase III transcription factor IIIB in its promoter complex
    S M Shah
    Department of Biology, Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
    J Biol Chem 274:28736-44. 1999
    ..Examination of two-cross-link products formed by DNA with adjacent and nearby photoactive nucleotides supports the conclusion that Brf and B" share an extended interface along the length of the TFIIIB-DNA complex...
  27. pmc Transcription of the T4 late genes
    E Peter Geiduschek
    Division of Biological Sciences, Section of Molecular Biology, University of California, San Diego, La Jolla, CA 92093 0634, USA
    Virol J 7:288. 2010
    ..The emphasis of this account is on the sites and mechanisms of actions of these three proteins, and on their roles in the formation of transcription-ready open T4 late promoter complexes...
  28. pmc Molecular genetic analysis of a prokaryotic transcriptional coactivator: functional domains of the bacteriophage T4 gene 33 protein
    J W Winkelman
    Department of Biology, University of California, San Diego, La Jolla 92093 0634
    J Bacteriol 176:1164-71. 1994
    ..The possible roles of gp33 in mediating late gene expression are discussed in the light of the identification of these functional domains...
  29. ncbi request reprint Abortive initiation by Saccharomyces cerevisiae RNA polymerase III
    P Bhargava
    Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
    J Biol Chem 274:26550-6. 1999
    ..This cleavage also occurs in the normal process of forming a productive elongation complex. The possible role of nucleolytic retraction in disengaging pol III from TFIIIB is discussed...
  30. ncbi request reprint TRF2, but not TBP, mediates the transcription of ribosomal protein genes
    Yuan Liang Wang
    Section of Molecular Biology, University of California at San Diego, La Jolla, California 92093, USA
    Genes Dev 28:1550-5. 2014
    ..Hence, a specialized TRF2-based RNA polymerase II system functions in the synthesis of RPs and complements the RNA polymerase I and III systems. ..