Research Topics
| G A KassavetisSummaryAffiliation: University of California Country: USA Publications
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Detail Information
Publications
Domains of the Brf component of RNA polymerase III transcription factor IIIB (TFIIIB): functions in assembly of TFIIIB-DNA complexes and recruitment of RNA polymerase to the promoterG A Kassavetis
Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla 92093 0634, USA
Mol Cell Biol 17:5299-306. 1997..These findings suggest a functional complementarity and reciprocity between the Brf and B" components of TFIIIB...- Transcription factor IIIB generates extended DNA interactions in RNA polymerase III transcription complexes on tRNA genesG A Kassavetis
Department of Biology, University of California, San Diego, La Jolla 92093
Mol Cell Biol 9:2551-66. 1989.... - Functional and structural organization of Brf, the TFIIB-related component of the RNA polymerase III transcription initiation complexG A Kassavetis
Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
Mol Cell Biol 18:5587-99. 1998..The constraints established by these analyses specify a global model of the functional segments of Brf and how they fit into the structure of the TFIIIB-DNA complex... 
S. cerevisiae TFIIIB is the transcription initiation factor proper of RNA polymerase III, while TFIIIA and TFIIIC are assembly factorsG A Kassavetis
Department of Biology, University of California, San Diego, La Jolla 92093
Cell 60:235-45. 1990..Pol III unwinds at least 14 bp of DNA at the SUP4 transcription start in a temperature-dependent process. The unwound DNA segment moves downstream with nascent RNA as a transcription bubble of approximately the same size...- Cloning, expression, and function of TFC5, the gene encoding the B" component of the Saccharomyces cerevisiae RNA polymerase III transcription factor TFIIIBG A Kassavetis
Department of Biology, University of California at San Diego, La Jolla 92093 0634, USA
Proc Natl Acad Sci U S A 92:9786-90. 1995..A truncated form of B" lacking 39 N-terminal and 107 C-terminal amino acids is also functional for transcription... - Saccharomyces cerevisiae transcription factors IIIB and IIIC bend the DNA of a tRNA(Gln) geneT Leveillard
Department of Biology, University of California, San Diego, La Jolla 92093
J Biol Chem 266:5162-8. 1991..Both transcription factors bend DNA, TFIIIB more than TFIIIC. The effective centers of the transcription factor IIIB- and IIIC-induced bends are not in helical phase... - The RNA polymerase III transcription initiation factor TFIIIB participates in two steps of promoter openingG A Kassavetis
Department of Biology and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
EMBO J 20:2823-34. 2001..Removing an N-terminal domain of Brf interferes with downstream propagation of the transcription bubble to and beyond the transcriptional start site... - The RNA polymerase III-recruiting factor TFIIIB induces a DNA bend between the TATA box and the transcriptional start siteA Grove
Department of Biology and Center for Molecular Genetics, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093 0634, USA
J Mol Biol 285:1429-40. 1999..We conclude that TFIIIB induces an additional DNA deformation between the TATA box and the start site of transcription that is likely to be more extended than the sharp kinks generated by TBP... - Bending of the Saccharomyces cerevisiae 5S rRNA gene in transcription factor complexesB R Braun
Department of Biology, University of California, San Diego, La Jolla 92093 0634
J Biol Chem 267:22562-9. 1992..Fully assembled transcription factor complexes bend the 5S rRNA gene in the same net direction as does TFIIIB alone... - A minimal RNA polymerase III transcription systemG A Kassavetis
Department of Biology, Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
EMBO J 18:5042-51. 1999.... - Alignment of the B" subunit of RNA polymerase III transcription factor IIIB in its promoter complexS M Shah
Department of Biology, Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
J Biol Chem 274:28736-44. 1999..Examination of two-cross-link products formed by DNA with adjacent and nearby photoactive nucleotides supports the conclusion that Brf and B" share an extended interface along the length of the TFIIIB-DNA complex... - Multiple states of protein-DNA interaction in the assembly of transcription complexes on Saccharomyces cerevisiae 5S ribosomal RNA genesB R Braun
Department of Biology, University of California, San Diego, La Jolla 92093
Proc Natl Acad Sci U S A 86:2530-4. 1989..Complexes assembled from the complete set of purified transcription factors or from a complete transcription system extend over the entire transcription unit together with almost 50 base pairs of 5' flanking sequence... - Topography of transcription factor complexes on the Saccharomyces cerevisiae 5 S RNA geneB R Braun
Department of Biology, University of California, San Diego, La Jolla 92093 0634
J Mol Biol 228:1063-77. 1992..A 90 kDa component that was not previously recognized as an integral part of TFIIIC has been specifically located at the 3' end of the 5 S rRNA gene... - A post-recruitment function for the RNA polymerase III transcription-initiation factor IIIBG A Kassavetis
Department of Biology and Center for Molecular Genetics, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
Proc Natl Acad Sci U S A 95:9196-201. 1998..It is proposed that the normal function of TFIIIB combines pol III recruitment with an active role in a subsequent step of transcriptional initiation leading to promoter opening... - Abortive initiation by Saccharomyces cerevisiae RNA polymerase IIIP Bhargava
Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
J Biol Chem 274:26550-6. 1999..This cleavage also occurs in the normal process of forming a productive elongation complex. The possible role of nucleolytic retraction in disengaging pol III from TFIIIB is discussed... - Transcription factor TFIIIB and transcription by RNA polymerase IIIG A Kassavetis
Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
Biochem Soc Trans 34:1082-7. 2006.... - Molecular genetic analysis of a prokaryotic transcriptional coactivator: functional domains of the bacteriophage T4 gene 33 proteinJ W Winkelman
Department of Biology, University of California, San Diego, La Jolla 92093 0634
J Bacteriol 176:1164-71. 1994..The possible roles of gp33 in mediating late gene expression are discussed in the light of the identification of these functional domains... - The role of the TATA-binding protein in the assembly and function of the multisubunit yeast RNA polymerase III transcription factor, TFIIIBG A Kassavetis
Department of Biology, University of California, San Diego, La Jolla 92093 0634
Cell 71:1055-64. 1992..Incorporation of the 90 kd B" protein into the transcription complex requires TBP. The heparin-resistant TFIIIB-DNA complex retains all three of its constituent proteins, TBP, BRF, and B"...