HOWARD KABACK

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint Structure and mechanism of the lactose permease
    H Ronald Kaback
    Department of Physiology and Microbiology, Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095 1662, USA
    C R Biol 328:557-67. 2005
  2. pmc The alternating access transport mechanism in LacY
    H Ronald Kaback
    Department of Physiology, University of California Los Angeles, Los Angeles, CA 90095, USA
    J Membr Biol 239:85-93. 2011
  3. pmc Site-directed alkylation and the alternating access model for LacY
    H Ronald Kaback
    Department of Physiology, University of California, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 104:491-4. 2007
  4. pmc Site-directed alkylation of LacY: effect of the proton electrochemical gradient
    Yiling Nie
    Department of Physiology, University of California Los Angeles, Los Angeles, CA 90095 1662, USA
    J Mol Biol 374:356-64. 2007
  5. pmc Binding affinity of lactose permease is not altered by the H+ electrochemical gradient
    Lan Guan
    Howard Hughes Medical Institute, Department of Physiology, Molecular Biology Institute, David Geffen School of Medicine, University of California, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 101:12148-52. 2004
  6. ncbi request reprint Interhelical packing modulates conformational flexibility in the lactose permease of Escherichia coli
    Natalia V Ermolova
    Department of Physiology, University of California Los Angeles, Los Angeles, California 90095 1662, USA
    Biochemistry 44:7669-77. 2005
  7. ncbi request reprint Characterization of a lactose permease mutant that binds IIAGlc in the absence of ligand
    Melissa Sondej
    Howard Hughes Medical Institute, Department of Physiology and Immunology, University of California, Los Angeles, California 90095 1662, USA
    Biochemistry 42:9153-9. 2003
  8. ncbi request reprint Helices VII and X in the lactose permease of Escherichia coli: proximity and ligand-induced distance changes
    Wei Zhang
    Howard Hughes Medical Institute, Departments of Physiology and Microbiology and Molecular Genetics Molecular Biology Institute, Los Angeles, CA, 90095 1622, USA
    J Mol Biol 315:53-62. 2002
  9. ncbi request reprint Aromatic stacking in the sugar binding site of the lactose permease
    Lan Guan
    Howard Hughes Medical Institute, Department of Physiology, Molecular Biology Institute, University of California Los Angeles, Los Angeles, California 90095 1662, USA
    Biochemistry 42:1377-82. 2003
  10. ncbi request reprint Binding of enzyme IIAGlc, a component of the phosphoenolpyruvate:sugar phosphotransferase system, to the Escherichia coli lactose permease
    Melissa Sondej
    Howard Hughes Medical Institute, Department of Physiology, University of California Los Angeles, Los Angeles, California 90095 1662, USA
    Biochemistry 41:5556-65. 2002

Research Grants

Collaborators

Detail Information

Publications60

  1. ncbi request reprint Structure and mechanism of the lactose permease
    H Ronald Kaback
    Department of Physiology and Microbiology, Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095 1662, USA
    C R Biol 328:557-67. 2005
    ..Based on the structure and a large body of biochemical and biophysical evidence, a mechanism is proposed in which the binding site is alternatively accessible to either side of the membrane...
  2. pmc The alternating access transport mechanism in LacY
    H Ronald Kaback
    Department of Physiology, University of California Los Angeles, Los Angeles, CA 90095, USA
    J Membr Biol 239:85-93. 2011
    ..Here, in honor of Stephan H. White's seventieth birthday, we review in camera the various biochemical/biophysical approaches that provide experimental evidence for the alternating access mechanism...
  3. pmc Site-directed alkylation and the alternating access model for LacY
    H Ronald Kaback
    Department of Physiology, University of California, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 104:491-4. 2007
    ....
  4. pmc Site-directed alkylation of LacY: effect of the proton electrochemical gradient
    Yiling Nie
    Department of Physiology, University of California Los Angeles, Los Angeles, CA 90095 1662, USA
    J Mol Biol 374:356-64. 2007
    ..The results suggest that Delta(mu (H)+), like sugar, acts to increase the probability of opening on the periplasmic side of LacY...
  5. pmc Binding affinity of lactose permease is not altered by the H+ electrochemical gradient
    Lan Guan
    Howard Hughes Medical Institute, Department of Physiology, Molecular Biology Institute, David Geffen School of Medicine, University of California, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 101:12148-52. 2004
    ....
  6. ncbi request reprint Interhelical packing modulates conformational flexibility in the lactose permease of Escherichia coli
    Natalia V Ermolova
    Department of Physiology, University of California Los Angeles, Los Angeles, California 90095 1662, USA
    Biochemistry 44:7669-77. 2005
    ..Rather, activity is dependent on more subtle interactions between the helices, and mutations that disrupt interactions between helix IV and loop 6-7 or between helices II and IV also rescue transport in the C154G mutant...
  7. ncbi request reprint Characterization of a lactose permease mutant that binds IIAGlc in the absence of ligand
    Melissa Sondej
    Howard Hughes Medical Institute, Department of Physiology and Immunology, University of California, Los Angeles, California 90095 1662, USA
    Biochemistry 42:9153-9. 2003
    ..The data are consistent with the interpretation that the double mutant is locked in an inward-facing conformation...
  8. ncbi request reprint Helices VII and X in the lactose permease of Escherichia coli: proximity and ligand-induced distance changes
    Wei Zhang
    Howard Hughes Medical Institute, Departments of Physiology and Microbiology and Molecular Genetics Molecular Biology Institute, Los Angeles, CA, 90095 1622, USA
    J Mol Biol 315:53-62. 2002
    ....
  9. ncbi request reprint Aromatic stacking in the sugar binding site of the lactose permease
    Lan Guan
    Howard Hughes Medical Institute, Department of Physiology, Molecular Biology Institute, University of California Los Angeles, Los Angeles, California 90095 1662, USA
    Biochemistry 42:1377-82. 2003
    ..The results are discussed in the context of a current model for the binding site...
  10. ncbi request reprint Binding of enzyme IIAGlc, a component of the phosphoenolpyruvate:sugar phosphotransferase system, to the Escherichia coli lactose permease
    Melissa Sondej
    Howard Hughes Medical Institute, Department of Physiology, University of California Los Angeles, Los Angeles, California 90095 1662, USA
    Biochemistry 41:5556-65. 2002
    ....
  11. pmc The Cys154-->Gly mutation in LacY causes constitutive opening of the hydrophilic periplasmic pathway
    Yiling Nie
    Department of Physiology, University of California Los Angeles, MacDonald Research Laboratories Rm 6720, 675 Charles E Young Drive South, Los Angeles, CA 90095 1662, USA
    J Mol Biol 379:695-703. 2008
    ....
  12. pmc Helix dynamics in LacY: helices II and IV
    Zhenyu Liu
    Department of Physiology, University of California, Los Angeles, Los Angeles, CA 90095 1662, USA
    J Mol Biol 396:617-26. 2010
    ..Evidence suggesting that movements of helices II and IV are coupled functionally with movements in the pseudo-symmetrically paired helices VIII and X is also presented...
  13. ncbi request reprint Exploring the role of a unique carboxyl residue in EmrE by mass spectrometry
    Adam B Weinglass
    Howard Hughes Medical Institute, Department of Physiology, Molecular Biology Institute, University of California Los Angeles, 90095 1662, USA
    J Biol Chem 280:7487-92. 2005
    ..Taken together with other biochemical data, the findings support a "time sharing" mechanism in which both Glu-14 residues in a dimer are involved in tetraphenylphosphonium and H(+) binding...
  14. pmc Exploiting luminescence spectroscopy to elucidate the interaction between sugar and a tryptophan residue in the lactose permease of Escherichia coli
    José Luis Vázquez-Ibar
    Howard Hughes Medical Institute, and Department of Physiology, Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 100:12706-11. 2003
    ..The spectroscopic data fully complement the x-ray structure and demonstrate the feasibility of fluorescence spectroscopy for studying sugar-protein interactions...
  15. pmc Conservation of residues involved in sugar/H(+) symport by the sucrose permease of Escherichia coli relative to lactose permease
    Viveka Vadyvaloo
    Department of Physiology and Microbiology, Immunology and Molecular Genetics, Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095 7327, USA
    J Mol Biol 358:1051-9. 2006
    ..Functional analyses of mutants in the homologous key residues provide strong evidence that they play a similar critical role in the mechanisms of CscB and LacY...
  16. ncbi request reprint A mutation in the lactose permease of Escherichia coli that decreases conformational flexibility and increases protein stability
    Irina N Smirnova
    Howard Hughes Medical Institute, Department of Physiology and Microbiology, University of California, Los Angeles, California 90095 1662, USA
    Biochemistry 42:3025-31. 2003
    ....
  17. pmc Site-directed alkylation of cysteine to test solvent accessibility of membrane proteins
    Lan Guan
    Department of Physiology, University of California Los Angeles, Los Angeles, California, USA
    Nat Protoc 2:2012-7. 2007
    ..Gels are dried and exposed to a PhosphoImager screen for 1-5 d, and incorporation of radioactivity is visualized. Initial results can be obtained in 24 h...
  18. pmc Structural determination of wild-type lactose permease
    Lan Guan
    Department of Physiology, University of California, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 104:15294-8. 2007
    ..Because the inward-facing conformation represents the lowest free-energy state, the rate-limiting step for transport may be the conformational change leading to the outward-facing conformation...
  19. ncbi request reprint Sugar recognition by the lactose permease of Escherichia coli
    José Luis Vázquez-Ibar
    Department of Physiology and Microbiology, Molecular Biology Institute, Howard Hughes Medical Institute, Los Angeles, CA 90095 1662, USA
    J Biol Chem 279:49214-21. 2004
    ....
  20. ncbi request reprint Site-directed sulfhydryl labeling of helix IX in the lactose permease of Escherichia coli
    Wei Zhang
    Howard Hughes Medical Institute, Department of Physiology and of Microbiology, Molecular Biology Institute, University of California, Los Angeles, California 90095 1662, USA
    Biochemistry 42:4904-8. 2003
    ..and Kaback, H. R. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 6068-6073], the findings are consistent with the idea that this face of helix IX may comprise part of the H(+) translocation pathway...
  21. pmc Direct sugar binding to LacY measured by resonance energy transfer
    Irina N Smirnova
    Department of Physiology and Microbiology, Immunology and Molecular Genetics, Molecular Biology Institute, University of California Los Angeles, Los Angeles, California 90095 7327, USA
    Biochemistry 45:15279-87. 2006
    ....
  22. ncbi request reprint Site-directed alkylation of cysteine replacements in the lactose permease of Escherichia coli: helices I, III, VI, and XI
    Natalia Ermolova
    Department of Physiology and Microbiology, University of California Los Angeles, Los Angeles, California 90095 1662, USA
    Biochemistry 45:4182-9. 2006
    ..The findings are consistent with the X-ray structure of LacY and with the alternating access model [Abramson, J., Smirnova, I., et al. (2003) Science 301, 610-615]...
  23. pmc Energetics of ligand-induced conformational flexibility in the lactose permease of Escherichia coli
    Yiling Nie
    Department of Physiology and Microbiology, Molecular Biology Institute, University of California Los Angeles, Los Angeles, California 90095, USA
    J Biol Chem 281:35779-84. 2006
    ..The findings also indicate that the structure of the mutant represents a conformational intermediate in the overall transport cycle...
  24. ncbi request reprint In vitro synthesis of lactose permease to probe the mechanism of membrane insertion and folding
    Shushi Nagamori
    Howard Hughes Medical Institute and Departments of Physiology and Microbiology, Immunology, and Molecular Genetics, Molecular Biology Institute, UCLA, Los Angeles, California 90095 1662, USA
    J Biol Chem 278:14820-6. 2003
    ..Furthermore, there is synergism between N(6) and the C-terminal half of permease during assembly, as opposed to assembly of the two halves as independent domains...
  25. ncbi request reprint Manipulating conformational equilibria in the lactose permease of Escherichia coli
    Adam B Weinglass
    Howard Hughes Medical Institute, Department of Physiology, Molecular Biology Institute, Los Angeles, CA 90095 1662, USA
    J Mol Biol 315:561-71. 2002
    ..When the two sets of mutants are combined, the equilibrium between outwardly and inwardly facing conformations and thus protonation and H(+) transfer are restored...
  26. pmc Elucidation of substrate binding interactions in a membrane transport protein by mass spectrometry
    Adam B Weinglass
    Department of Physiology, Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095 1662, USA
    EMBO J 22:1467-77. 2003
    ..The approach demonstrates that mass spectrometry can provide a powerful means of analyzing ligand interactions with integral membrane proteins...
  27. pmc Changing the lactose permease of Escherichia coli into a galactose-specific symporter
    Lan Guan
    Howard Hughes Medical Institute, Departments of Physiology and Microbiology and Molecular Genetics, Molecular Biology Institute, University of California, Los Angeles, CA 90095 1662
    Proc Natl Acad Sci U S A 99:6613-8. 2002
    ..This is in contrast to Cys-148, a neighboring residue in helix V, that interacts with the hydrophobic face of the galactosyl moiety of D-galactopyranosides...
  28. pmc Role of YidC in folding of polytopic membrane proteins
    Shushi Nagamori
    5 748 MacDonald Research Laboratories, Rm 6720, P O Box 951662, Howard Hughes Medical Institute, University of California, Los Angeles, Los Angeles, CA 90095 1662, USA
    J Cell Biol 165:53-62. 2004
    ..The results indicate that YidC plays a primary role in folding of LacY into its final tertiary conformation via an interaction that likely occurs transiently during insertion into the lipid phase of the membrane...
  29. pmc Surface-exposed positions in the transmembrane helices of the lactose permease of Escherichia coli determined by intermolecular thiol cross-linking
    Lan Guan
    Howard Hughes Medical Institute, Department of Physiology, Molecular Biology Institute, University of California, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 99:3475-80. 2002
    ..The findings confirm previous observations indicating that intermolecular cross-linking is a stochastic process resulting from random collisions and support a number of other lines of evidence that lactose permease is a monomer...
  30. pmc Engineering a terbium-binding site into an integral membrane protein for luminescence energy transfer
    José Luis Vázquez-Ibar
    Howard Hughes Medical Institute, Department of Physiology, Molecular Biology Institute, University of California, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 99:3487-92. 2002
    ..The approach should be of general use for studying static and dynamic aspects of polytopic membrane protein structure and function...
  31. ncbi request reprint Probing the mechanism of a membrane transport protein with affinity inactivators
    Lan Guan
    Howard Hughes Medical Institute, Department of Physiology, UCLA, Los Angeles, California 90095 1662, USA
    J Biol Chem 278:10641-8. 2003
    ..Thus, these inactivators behave as unique suicide substrates...
  32. pmc Intermolecular thiol cross-linking via loops in the lactose permease of Escherichia coli
    Natalia Ermolova
    Howard Hughes Medical Institute, Department of Physiology and Microbiology, University of California, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 100:10187-92. 2003
    ..In addition, evidence is presented indicating that reentrance loops are unlikely in this polytopic membrane transport protein...
  33. pmc Protonation and sugar binding to LacY
    Irina N Smirnova
    Department of Physiology and Microbiology, Molecular Biology Institute, University of California, Los Angeles, CA 90095 7327, USA
    Proc Natl Acad Sci U S A 105:8896-901. 2008
    ..The results are consistent with the conclusion that LacY is protonated before sugar binding during lactose/H(+) symport in either direction across the membrane...
  34. pmc Sequence alignment and homology threading reveals prokaryotic and eukaryotic proteins similar to lactose permease
    Vladimir N Kasho
    Department of Physiology and Microbiology, Immunology and Molecular Genetics, Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095 7327, USA
    J Mol Biol 358:1060-70. 2006
    ..Homology is observed in the TMDs of both the eukaryotic and prokaryotic proteins that contain residues involved in sugar binding and H(+) translocation in LacY...
  35. pmc Residues in the H+ translocation site define the pKa for sugar binding to LacY
    Irina Smirnova
    Department of Physiology, University of California Los Angeles, Los Angeles, California 90095 7327, USA
    Biochemistry 48:8852-60. 2009
    ..There is structural evidence for water bound in this complex, and the water could be the site of protonation responsible for the pH dependence of sugar binding...
  36. pmc Properties of a LacY efflux mutant
    Lan Guan
    Department of Physiology, University of California Los Angeles, Los Angeles, California 90095 1662, USA
    Biochemistry 48:9250-5. 2009
    ..Taken as a whole, the results suggest that the primary defect induced by the mutation may involve a decrease in affinity for H(+)...
  37. pmc Residues gating the periplasmic pathway of LacY
    Yonggang Zhou
    Department of Physiology, University of California, Los Angeles, CA 90095 7327, USA
    J Mol Biol 394:219-25. 2009
    ..The data indicate that residues Ile40 and Asn245 play a primary role in gating the periplasmic cavity and provide further support for the alternating-access model...
  38. ncbi request reprint Monitoring conformational rearrangements in the substrate-binding site of a membrane transport protein by mass spectrometry
    Adam Weinglass
    Howard Hughes Medical Institute and Department of Physiology and Microbiology, University of California, Los Angeles, Los Angeles, CA 90095, USA
    J Biol Chem 279:41858-65. 2004
    ....
  39. pmc Sugar binding induces an outward facing conformation of LacY
    Irina Smirnova
    Department of Physiology, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 104:16504-9. 2007
    ..The DEER measurements in conjunction with molecular modeling based on the x-ray structure provide strong support for the alternative access model and reveal a structure for the outward-facing conformer of LacY...
  40. pmc Clogging the periplasmic pathway in LacY
    Yiling Nie
    Department of Physiology, Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
    Biochemistry 48:738-43. 2009
    ....
  41. ncbi request reprint Binding of hydrophobic D-galactopyranosides to the lactose permease of Escherichia coli
    Miklos Sahin-Toth
    Howard Hughes Medical Institute, Department of Physiology, Molecular Biology Institute, University of California, Los Angeles, California 90095 1662, USA
    Biochemistry 41:13039-45. 2002
    ..The different positioning of this carbon atom in alpha- or beta-D-galactopyranosides thus may provide a rationale for the characteristic binding preference of the permease for alpha anomers...
  42. ncbi request reprint Integrating mass spectrometry into membrane protein drug discovery
    Adam B Weinglass
    Howard Hughes Medical Institute, Departments of Physiology and Microbiology and Molecular Genetics Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095 1662, USA
    Curr Opin Drug Discov Devel 7:589-99. 2004
    ....
  43. pmc Manipulating phospholipids for crystallization of a membrane transport protein
    Lan Guan
    Department of Physiology, University of California, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 103:1723-6. 2006
    ..Thus, it is likely that manipulation of PL is a good strategy for predominantly hydrophobic membrane proteins like LacY...
  44. pmc Lessons from lactose permease
    Lan Guan
    Department of Physiology, University of California, Los Angeles, California 90095 1662, USA
    Annu Rev Biophys Biomol Struct 35:67-91. 2006
    ..A working model for the mechanism is presented that involves alternating access of both the sugar- and H+-binding sites to either side of the membrane...
  45. pmc Sugar binding induces the same global conformational change in purified LacY as in the native bacterial membrane
    Yiling Nie
    Department of Physiology, Department of Microbiology, Immunology and Molecular Genetics, and Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 107:9903-8. 2010
    ..The results also indicate that the x-ray crystal structure reflects the structure of wild-type LacY in the native membrane in the absence of sugar...
  46. pmc Probing of the rates of alternating access in LacY with Trp fluorescence
    Irina Smirnova
    Department of Physiology, University of California, Los Angeles, CA 90095 7327, USA
    Proc Natl Acad Sci U S A 106:21561-6. 2009
    ....
  47. pmc Opening and closing of the periplasmic gate in lactose permease
    Yonggang Zhou
    Departments of Physiology and Microbiology, Immunology, and Molecular Genetics, Molecular Biology Institute, University of California, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 105:3774-8. 2008
    ..The results provide direct support for the argument that transport via LacY involves opening and closing of a large periplasmic cavity...
  48. pmc Single-molecule FRET reveals sugar-induced conformational dynamics in LacY
    Devdoot S Majumdar
    Molecular Biology Institute, Department of Physiology, California NanoSystems Institute, University of California, Los Angeles, CA 90095 1662, USA
    Proc Natl Acad Sci U S A 104:12640-5. 2007
    ..The results are consistent with the alternating access model and indicate that the defect in the mutant is due to impaired ligand-induced flexibility on the periplasmic side...
  49. ncbi request reprint Lactose permease as a paradigm for membrane transport proteins (Review)
    Jeff Abramson
    Department of Biological Sciences, Imperial College London, London SW7 2AZ, UK
    Mol Membr Biol 21:227-36. 2004
    ..In addition, we make some brief comparisons between the structures of LacY and GlpT...
  50. ncbi request reprint Structural comparison of lactose permease and the glycerol-3-phosphate antiporter: members of the major facilitator superfamily
    Jeff Abramson
    Department of Biological Sciences, Imperial College London, London SW7 2AZ, UK
    Curr Opin Struct Biol 14:413-9. 2004
    ..All three structures reveal 12 transmembrane helices forming two distinct domains, and could imply that members of this superfamily have preserved both secondary and tertiary structure elements during evolution...
  51. ncbi request reprint Asymmetric binding of membrane proteins to GroEL
    Jingchuan Sun
    Microscopy and Imaging Center, Texas A and M University, College Station, TX 77843 2257, USA
    Arch Biochem Biophys 434:352-7. 2005
    ..Each of the membrane proteins was visualized in one of the center cavities of GroEL using single particle analysis...
  52. ncbi request reprint The lactose permease of Escherichia coli: overall structure, the sugar-binding site and the alternating access model for transport
    Jeff Abramson
    Department of Biological Sciences, Imperial College London, London SW7 2AZ, UK
    FEBS Lett 555:96-101. 2003
    ....
  53. ncbi request reprint Structure and mechanism of the lactose permease of Escherichia coli
    Jeff Abramson
    Department of Biological Sciences, Imperial College London, London SW7 2AZ, UK
    Science 301:610-5. 2003
    ..We propose a possible mechanism for lactose/proton symport (co-transport) consistent with both the structure and a large body of experimental data...
  54. pmc Functional overlap but lack of complete cross-complementation of Streptococcus mutans and Escherichia coli YidC orthologs
    Yuxia Dong
    Department of Chemistry, The Ohio State University, Columbus, Ohio 43210, USA
    J Bacteriol 190:2458-69. 2008
    ....
  55. pmc Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY
    Osman Mirza
    Department of Biological Sciences, Membrane Protein Crystallography Group, Imperial College London, London, UK
    EMBO J 25:1177-83. 2006
    ..Thus, substrate induces formation of the sugar-binding site, as well as the initial step in H+ transduction...
  56. ncbi request reprint Ligand-induced differences in secondary structure of the Vibrio parahaemolyticus Na+/galactose cotransporter
    Johannes le Coutre
    Nestlé Research Center Lausanne, Vers chez les Blanc, 1000 Lausanne 26, Switzerland
    Biochemistry 41:8082-6. 2002
    ..The data demonstrate discrete conformational changes from one intermediate to the next during the catalytic cycle of the protein and are interpreted in a model of the symport reaction mechanism...
  57. pmc SecY alterations that impair membrane protein folding and generate a membrane stress
    Nobuyuki Shimohata
    Institute for Virus Research, Kyoto University, Sakyo ku, Kyoto 606 8507, Japan
    J Cell Biol 176:307-17. 2007
    ..The results demonstrate that normal SecY function is required for the folding of membrane proteins after their insertion into the translocon...
  58. ncbi request reprint The Sec-independent function of Escherichia coli YidC is evolutionary-conserved and essential
    Edwin van Bloois
    Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands
    J Biol Chem 280:12996-3003. 2005
    ..Together, our data indicate that the Sec-independent function of YidC is conserved and essential for cell growth...
  59. pmc An approach to membrane protein structure without crystals
    Paul L Sorgen
    Biochemistry Department, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 99:14037-40. 2002
    ..Here we demonstrate that this information is sufficient to calculate a structural model at the level of helix packing or better...
  60. pmc Structural conservation in the major facilitator superfamily as revealed by comparative modeling
    Eyal Vardy
    Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, 91904 Israel
    Protein Sci 13:1832-40. 2004
    ..The models are based on the two MFS structures and on experimental data. The models for both proteins are in good agreement with the data available and support the notion of a shared fold for all MFS proteins...

Research Grants18

  1. Structural Basis for Mechanism of Secondary Transporters
    HOWARD RONALD KABACK; Fiscal Year: 2010
    ..In order to understand its mechanism of action, however, it is essential to obtain structures of LacY in more than the single form that we have obtained, which is the purpose of this proposal. ..
  2. Dynamics of the Lactose Permease of Escherichia coli
    HOWARD KABACK; Fiscal Year: 2009
    ..abstract_text> ..
  3. Dynamics of the Lactose Permease of Escherichia coli
    H Kaback; Fiscal Year: 2007
    ..abstract_text> ..
  4. Structural Basis for Mechanism of Secondary Transporters
    H Kaback; Fiscal Year: 2007
    ..X-ray structures of these transport proteins in multiple conformations will have important impact on biology and medicine. ..
  5. New Approaches to Membrane Protein Structure
    HOWARD KABACK; Fiscal Year: 2004
    ..Site-specific alkylation combined with mass spectrometry will be used to determine changes in the protonation of His322 (helix X) upon ligand binding. ..
  6. NEW APPROACHES TO MEMBRANE PROTEIN STRUCTURES
    H Kaback; Fiscal Year: 2000
    ....
  7. Dynamics of the Lactose Permease of Escherichia coli
    HOWARD RONALD KABACK; Fiscal Year: 2010
    ....