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Genomes and Genes | M K JohnsonSummaryAffiliation: University of Georgia Country: USA Publications
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Publications
Iron-sulfur proteins: new roles for old clustersM K Johnson
Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, GA 30602, USA
Curr Opin Chem Biol 2:173-81. 1998....- Effect of serinate ligation at each of the iron sites of the [Fe4S4] cluster of Pyrococcus furiosus ferredoxin on the redox, spectroscopic, and biological propertiesP S Brereton
Department of Biochemistry, Center for Metalloenzyme Studies, University of Georgia, Athens 30602, USA
Biochemistry 38:10594-605. 1999..furiosus. Hence, the mode of ligation at each Fe atom in the [Fe(4)S(4)] cluster appears to have little effect on the interaction and the electron transfer between Fd and FNOR... - Sulfur transfer from IscS to IscU: the first step in iron-sulfur cluster biosynthesisA D Smith
Department of Chemistry, Center for Metalloenzyme Studies, University of Georgia, Athens, GA 30602, USA
J Am Chem Soc 123:11103-4. 2001 - Evidence that the fourth ligand to the [2Fe-2S] cluster in animal ferrochelatase is a cysteine. Characterization of the enzyme from Drosophila melanogasterV M Sellers
Department of Microbiology, and the Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
J Biol Chem 273:22311-6. 1998.... - Site-directed mutagenesis and spectroscopic characterization of human ferrochelatase: identification of residues coordinating the [2Fe-2S] clusterB R Crouse
Department of Microbiology, University of Georgia, Athens 30602, USA
Biochemistry 35:16222-9. 1996..Such anomalous coordination could account for the cluster lability compared to similar clusters with complete cysteinyl ligation and hence may be intrinsic to the proposed regulatory role for this cluster in mammalian ferrochelatases... - [2Fe-2S] to [4Fe-4S] cluster conversion in Escherichia coli biotin synthaseE C Duin
Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
Biochemistry 36:11811-20. 1997.... - Role of the [Fe4S4] cluster in mediating disulfide reduction in spinach ferredoxin:thioredoxin reductaseC R Staples
Department of Chemistry and the Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
Biochemistry 37:4612-20. 1998.... - Superoxide reductase: fact or fiction?Michael W W Adams
Department of Biochemistry and Molecular Biology, University of Georgia, Athens 30602, USA
J Biol Inorg Chem 7:647-52. 2002..In particular, the role of otherwise well-characterized proteins like rubrerythrin, NADH peroxidase, and rubredoxin:oxygen oxidoreductase in "anaerobic" oxygen metabolism has yet to be established... - A pure S = 3/2 [Fe4S4]+ cluster in the A33Y variant of Pyrococcus furiosus ferredoxinR E Duderstadt
Department of Chemistry, Center for Metalloenzyme Studies, University of Georgia, Athens 30602, USA
FEBS Lett 454:21-6. 1999.... - IscU as a scaffold for iron-sulfur cluster biosynthesis: sequential assembly of [2Fe-2S] and [4Fe-4S] clusters in IscUJ N Agar
Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
Biochemistry 39:7856-62. 2000.... - Function of the [2FE-2S] cluster in mammalian ferrochelatase: a possible role as a nitric oxide sensorV M Sellers
Department of Microbiology, University of Georgia, Athens, Georgia 30602, USA
Biochemistry 35:2699-704. 1996..The potential physiological relevance of these data to the anemias that are found in individuals with chronic infections is discussed... - Spectroscopic characterization of the [Fe(His)(4)(Cys)] site in 2Fe-superoxide reductase from Desulfovibrio vulgarisMichael D Clay
Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, GA 30602, USA
J Biol Inorg Chem 8:671-82. 2003..furiosus 1Fe-SOR and D. vulgaris 2Fe-SOR has confirmed the existence of novel ferrocyanide adducts of the ferric [Fe(His)(4)(Cys)] sites in both 1Fe- and 2Fe-SORs... 
Geometries and electronic structures of cyanide adducts of the non-heme iron active site of superoxide reductases: vibrational and ENDOR studiesMichael D Clay
Department of Chemistry, University of Georgia, Athens, Georgia 30602, USA
Biochemistry 45:427-38. 2006..The implications of these results for understanding the mechanism of SOR are discussed...- Spectroscopic studies of Pyrococcus furiosus superoxide reductase: implications for active-site structures and the catalytic mechanismMichael D Clay
Department of Chemistry and the Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
J Am Chem Soc 124:788-805. 2002..The structural and electronic properties of the SOR active site are discussed in relation to the role and bonding of the axial cysteine residue and the recent proposals for the catalytic mechanism... - Resonance Raman characterization of the mononuclear iron active-site vibrations and putative electron transport pathways in Pyrococcus furiosus superoxide reductaseMichael D Clay
Department of Chemistry, Center for Metalloenzyme Studies, University of Georgia, Athens, GA 30602, USA
Biochemistry 41:9833-41. 2002.... - Nitric oxide binding at the mononuclear active site of reduced Pyrococcus furiosus superoxide reductaseMichael D Clay
Department of Chemistry, University of Georgia, Athens, GA 30602, USA
Proc Natl Acad Sci U S A 100:3796-801. 2003.... - Effect of cysteine to serine mutations on the properties of the [4Fe-4S] center in Escherichia coli fumarate reductaseA T Kowal
Department of Chemistry, University of Georgia, Athens 30602, USA
Biochemistry 34:12284-93. 1995..The role of the low potential [4Fe-4S] cluster in mediating electron transfer from menaquinol to the FAD active site is discussed in light of these mutagenesis results... - Spectroscopic characterization of site-specific [Fe(4)S(4)] cluster chemistry in ferredoxin:thioredoxin reductase: implications for the catalytic mechanismElizabeth M Walters
Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
J Am Chem Soc 127:9612-24. 2005..These results provide molecular level insights into the catalytic mechanism of FTR, and two viable mechanisms are proposed... - Comparing the electronic properties of the low-spin cyano-ferric [Fe(N4)(Cys)] active sites of superoxide reductase and p450cam using ENDOR spectroscopy and DFT calculationsTran-Chin Yang
Department of Chemistry and Biochemistry, Center for Metalloenzyme Studies, University of Georgia, Athens, GA 30602, USA
J Am Chem Soc 128:16566-78. 2006.... - Functional, structural, and spectroscopic characterization of a glutathione-ligated [2Fe-2S] cluster in poplar glutaredoxin C1Nicolas Rouhier
Unité Mixte de Recherche 1136, Institut National de la Recherche Agronomique, Institut Fédératif de Recherche 110, Genomics, Ecology, Nancy University, BP 239, 54506 Vandoeuvre les Nancy Cedex, France
Proc Natl Acad Sci U S A 104:7379-84. 2007..In light of these results, the possible involvement of plant glutaredoxins in oxidative stress sensing or iron-sulfur biosynthesis is discussed with respect to their intracellular localization... - Insights into the role of nucleotide-dependent conformational change in nitrogenase catalysis: Structural characterization of the nitrogenase Fe protein Leu127 deletion variant with bound MgATPSanchayita Sen
Department of Chemistry and Biochemistry, Montana State University, 108 Gaines Hall, Bozeman, MT 59717, USA
J Inorg Biochem 100:1041-52. 2006..This could be critical to the tight control of MgATP hydrolysis observed within the nitrogenase complex and may be important for maintaining unidirectional electron flow toward substrate reduction... - Heterodisulfide reductase from Methanothermobacter marburgensis contains an active-site [4Fe-4S] cluster that is directly involved in mediating heterodisulfide reductionEvert C Duin
Max Planck Institut für Terrestrische Mikrobiologie and Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, Karl von Frisch Strasse, D 35043, Marburg, Germany
FEBS Lett 512:263-8. 2002..The catalytic mechanism of HDR is discussed in light of the crystallographic and spectroscopic studies of the related chloroplast ferredoxin:thioredoxin reductase class of disulfide reductases... - Spectroscopic evidence for site specific chemistry at a unique iron site of the [4Fe-4S] cluster in ferredoxin:thioredoxin reductaseGuy N L Jameson
Department of Physics, Emory University, Atlanta, Georgia 30322, USA
J Am Chem Soc 125:1146-7. 2003.... - Spectroscopic investigation of the nickel-containing porphinoid cofactor F(430). Comparison of the free cofactor in the (+)1, (+)2 and (+)3 oxidation states with the cofactor bound to methyl-coenzyme M reductase in the silent, red and ox formsEvert C Duin
Department of Chemistry and Biochemistry, Auburn University, Auburn, AL 36849, USA
J Biol Inorg Chem 9:563-76. 2004..The reinterpretation of MCRox1 as a formal Ni(III) species rather than an Ni(I) species obviates the need to invoke a two-electron reduction of the F(430) macrocyclic ligand on reductive activation of MCRox1 to yield MCRred1... - Iron-sulfur cluster assembly: NifU-directed activation of the nitrogenase Fe proteinPatricia C Dos Santos
Department of Biochemistry, Virginia Tech, Blacksburg, Virginia 24061, USA
J Biol Chem 279:19705-11. 2004..These in vivo experiments were supported by in vitro [Fe-S] cluster assembly and transfer experiments involving the activation of an apo-form of the nitrogenase Fe protein... - A conformational mimic of the MgATP-bound "on state" of the nitrogenase iron proteinSanchayita Sen
Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717, USA
Biochemistry 43:1787-97. 2004.... - Biotin sulfoxide reductase: Tryptophan 90 is required for efficient substrate utilizationVeronica V Pollock
Department of Biochemistry and Molecular Biology, College of Medicine, University of South Florida, Tampa, FL 33612, USA
Arch Biochem Biophys 409:315-26. 2003..These results strongly suggest that W90 in BSOR plays a critical role in catalysis by serving as a hydrogen bond donor to the oxo group on the Mo(VI) center... - Sulfur K-edge X-ray absorption spectroscopy and density functional theory calculations on superoxide reductase: role of the axial thiolate in reactivityAbhishek Dey
Department of Chemistry, Stanford University, Stanford, California 94305, USA
J Am Chem Soc 129:12418-31. 2007..However, in contrast to FeSOD, the 5C FeIII site of SOR, which is more positively charged, is calculated to have a high affinity for binding a sixth anionic ligand, which would inhibit its SOD activity...