M K Johnson

Summary

Affiliation: University of Georgia
Country: USA

Publications

  1. ncbi request reprint Iron-sulfur proteins: new roles for old clusters
    M K Johnson
    Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, GA 30602, USA
    Curr Opin Chem Biol 2:173-81. 1998
  2. ncbi request reprint Effect of serinate ligation at each of the iron sites of the [Fe4S4] cluster of Pyrococcus furiosus ferredoxin on the redox, spectroscopic, and biological properties
    P S Brereton
    Department of Biochemistry, Center for Metalloenzyme Studies, University of Georgia, Athens 30602, USA
    Biochemistry 38:10594-605. 1999
  3. ncbi request reprint Sulfur transfer from IscS to IscU: the first step in iron-sulfur cluster biosynthesis
    A D Smith
    Department of Chemistry, Center for Metalloenzyme Studies, University of Georgia, Athens, GA 30602, USA
    J Am Chem Soc 123:11103-4. 2001
  4. ncbi request reprint Evidence that the fourth ligand to the [2Fe-2S] cluster in animal ferrochelatase is a cysteine. Characterization of the enzyme from Drosophila melanogaster
    V M Sellers
    Department of Microbiology, and the Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
    J Biol Chem 273:22311-6. 1998
  5. ncbi request reprint Site-directed mutagenesis and spectroscopic characterization of human ferrochelatase: identification of residues coordinating the [2Fe-2S] cluster
    B R Crouse
    Department of Microbiology, University of Georgia, Athens 30602, USA
    Biochemistry 35:16222-9. 1996
  6. ncbi request reprint [2Fe-2S] to [4Fe-4S] cluster conversion in Escherichia coli biotin synthase
    E C Duin
    Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
    Biochemistry 36:11811-20. 1997
  7. ncbi request reprint Role of the [Fe4S4] cluster in mediating disulfide reduction in spinach ferredoxin:thioredoxin reductase
    C R Staples
    Department of Chemistry and the Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
    Biochemistry 37:4612-20. 1998
  8. ncbi request reprint Superoxide reductase: fact or fiction?
    Michael W W Adams
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens 30602, USA
    J Biol Inorg Chem 7:647-52. 2002
  9. ncbi request reprint A pure S = 3/2 [Fe4S4]+ cluster in the A33Y variant of Pyrococcus furiosus ferredoxin
    R E Duderstadt
    Department of Chemistry, Center for Metalloenzyme Studies, University of Georgia, Athens 30602, USA
    FEBS Lett 454:21-6. 1999
  10. ncbi request reprint IscU as a scaffold for iron-sulfur cluster biosynthesis: sequential assembly of [2Fe-2S] and [4Fe-4S] clusters in IscU
    J N Agar
    Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
    Biochemistry 39:7856-62. 2000

Collaborators

  • Michael W W Adams
  • Donald M Kurtz
  • Peter L Hagedoorn
  • Graham N George
  • John Peters
  • George Georgiou
  • Evert C Duin
  • Nicholas Rouhier
  • Reiner Hedderich
  • H A Dailey
  • Rudolf K Thauer
  • Michael D Clay
  • Michael W W Adams
  • Francis E Jenney
  • Sanchayita Sen
  • Tran Chin Yang
  • Elizabeth M Walters
  • Guy N L Jameson
  • V M Sellers
  • Abhishek Dey
  • Brian M Hoffman
  • Rangan Krishnan
  • J N Agar
  • Lance C Seefeldt
  • Christopher A Cosper
  • Boi Hanh Huynh
  • Wanda Manieri
  • Patricia C Dos Santos
  • Peter Schürmann
  • P S Brereton
  • R E Duderstadt
  • Veronica V Pollock
  • C R Staples
  • A D Smith
  • J Frazzon
  • D R Dean
  • M W Adams
  • Edward I Solomon
  • Britt Hedman
  • Keith O Hodgson
  • Arathi Krishnakumar
  • Irene Y Kung
  • Robert K Szilagyi
  • Jammi McClead
  • Rebecca L McNaughton
  • Dominique A Glauser
  • Florence Bourquin
  • Ricardo Garcia-Serres
  • Robert Igarashi
  • Jeverson Frazzon
  • Archer D Smith
  • Valerie L Cash
  • Dennis R Dean
  • Archer Smith
  • Eric D Coulter
  • Michael J Barber
  • Richard C Conover
  • Joseph P Emerson
  • Hak Joon Noh
  • B R Crouse
  • K A Johnson
  • I J Amster
  • B H Huynh
  • A T Kowal
  • C Krebs
  • P Schürmann
  • K F Wang
  • J Telser
  • D B Knaff
  • A L Stritt-Etter
  • E Gaymard
  • B M Hoffman
  • M G Finnegan
  • A Manodori
  • I Schröder
  • R P Gunsalus
  • M T Werth
  • G Cecchini

Detail Information

Publications28

  1. ncbi request reprint Iron-sulfur proteins: new roles for old clusters
    M K Johnson
    Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, GA 30602, USA
    Curr Opin Chem Biol 2:173-81. 1998
    ....
  2. ncbi request reprint Effect of serinate ligation at each of the iron sites of the [Fe4S4] cluster of Pyrococcus furiosus ferredoxin on the redox, spectroscopic, and biological properties
    P S Brereton
    Department of Biochemistry, Center for Metalloenzyme Studies, University of Georgia, Athens 30602, USA
    Biochemistry 38:10594-605. 1999
    ..furiosus. Hence, the mode of ligation at each Fe atom in the [Fe(4)S(4)] cluster appears to have little effect on the interaction and the electron transfer between Fd and FNOR...
  3. ncbi request reprint Sulfur transfer from IscS to IscU: the first step in iron-sulfur cluster biosynthesis
    A D Smith
    Department of Chemistry, Center for Metalloenzyme Studies, University of Georgia, Athens, GA 30602, USA
    J Am Chem Soc 123:11103-4. 2001
  4. ncbi request reprint Evidence that the fourth ligand to the [2Fe-2S] cluster in animal ferrochelatase is a cysteine. Characterization of the enzyme from Drosophila melanogaster
    V M Sellers
    Department of Microbiology, and the Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
    J Biol Chem 273:22311-6. 1998
    ....
  5. ncbi request reprint Site-directed mutagenesis and spectroscopic characterization of human ferrochelatase: identification of residues coordinating the [2Fe-2S] cluster
    B R Crouse
    Department of Microbiology, University of Georgia, Athens 30602, USA
    Biochemistry 35:16222-9. 1996
    ..Such anomalous coordination could account for the cluster lability compared to similar clusters with complete cysteinyl ligation and hence may be intrinsic to the proposed regulatory role for this cluster in mammalian ferrochelatases...
  6. ncbi request reprint [2Fe-2S] to [4Fe-4S] cluster conversion in Escherichia coli biotin synthase
    E C Duin
    Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
    Biochemistry 36:11811-20. 1997
    ....
  7. ncbi request reprint Role of the [Fe4S4] cluster in mediating disulfide reduction in spinach ferredoxin:thioredoxin reductase
    C R Staples
    Department of Chemistry and the Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
    Biochemistry 37:4612-20. 1998
    ....
  8. ncbi request reprint Superoxide reductase: fact or fiction?
    Michael W W Adams
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens 30602, USA
    J Biol Inorg Chem 7:647-52. 2002
    ..In particular, the role of otherwise well-characterized proteins like rubrerythrin, NADH peroxidase, and rubredoxin:oxygen oxidoreductase in "anaerobic" oxygen metabolism has yet to be established...
  9. ncbi request reprint A pure S = 3/2 [Fe4S4]+ cluster in the A33Y variant of Pyrococcus furiosus ferredoxin
    R E Duderstadt
    Department of Chemistry, Center for Metalloenzyme Studies, University of Georgia, Athens 30602, USA
    FEBS Lett 454:21-6. 1999
    ....
  10. ncbi request reprint IscU as a scaffold for iron-sulfur cluster biosynthesis: sequential assembly of [2Fe-2S] and [4Fe-4S] clusters in IscU
    J N Agar
    Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
    Biochemistry 39:7856-62. 2000
    ....
  11. ncbi request reprint Function of the [2FE-2S] cluster in mammalian ferrochelatase: a possible role as a nitric oxide sensor
    V M Sellers
    Department of Microbiology, University of Georgia, Athens, Georgia 30602, USA
    Biochemistry 35:2699-704. 1996
    ..The potential physiological relevance of these data to the anemias that are found in individuals with chronic infections is discussed...
  12. ncbi request reprint Spectroscopic characterization of the [Fe(His)(4)(Cys)] site in 2Fe-superoxide reductase from Desulfovibrio vulgaris
    Michael D Clay
    Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, GA 30602, USA
    J Biol Inorg Chem 8:671-82. 2003
    ..furiosus 1Fe-SOR and D. vulgaris 2Fe-SOR has confirmed the existence of novel ferrocyanide adducts of the ferric [Fe(His)(4)(Cys)] sites in both 1Fe- and 2Fe-SORs...
  13. pmc Geometries and electronic structures of cyanide adducts of the non-heme iron active site of superoxide reductases: vibrational and ENDOR studies
    Michael D Clay
    Department of Chemistry, University of Georgia, Athens, Georgia 30602, USA
    Biochemistry 45:427-38. 2006
    ..The implications of these results for understanding the mechanism of SOR are discussed...
  14. ncbi request reprint Spectroscopic studies of Pyrococcus furiosus superoxide reductase: implications for active-site structures and the catalytic mechanism
    Michael D Clay
    Department of Chemistry and the Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
    J Am Chem Soc 124:788-805. 2002
    ..The structural and electronic properties of the SOR active site are discussed in relation to the role and bonding of the axial cysteine residue and the recent proposals for the catalytic mechanism...
  15. ncbi request reprint Resonance Raman characterization of the mononuclear iron active-site vibrations and putative electron transport pathways in Pyrococcus furiosus superoxide reductase
    Michael D Clay
    Department of Chemistry, Center for Metalloenzyme Studies, University of Georgia, Athens, GA 30602, USA
    Biochemistry 41:9833-41. 2002
    ....
  16. pmc Nitric oxide binding at the mononuclear active site of reduced Pyrococcus furiosus superoxide reductase
    Michael D Clay
    Department of Chemistry, University of Georgia, Athens, GA 30602, USA
    Proc Natl Acad Sci U S A 100:3796-801. 2003
    ....
  17. ncbi request reprint Effect of cysteine to serine mutations on the properties of the [4Fe-4S] center in Escherichia coli fumarate reductase
    A T Kowal
    Department of Chemistry, University of Georgia, Athens 30602, USA
    Biochemistry 34:12284-93. 1995
    ..The role of the low potential [4Fe-4S] cluster in mediating electron transfer from menaquinol to the FAD active site is discussed in light of these mutagenesis results...
  18. ncbi request reprint Spectroscopic characterization of site-specific [Fe(4)S(4)] cluster chemistry in ferredoxin:thioredoxin reductase: implications for the catalytic mechanism
    Elizabeth M Walters
    Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
    J Am Chem Soc 127:9612-24. 2005
    ..These results provide molecular level insights into the catalytic mechanism of FTR, and two viable mechanisms are proposed...
  19. ncbi request reprint Comparing the electronic properties of the low-spin cyano-ferric [Fe(N4)(Cys)] active sites of superoxide reductase and p450cam using ENDOR spectroscopy and DFT calculations
    Tran Chin Yang
    Department of Chemistry and Biochemistry, Center for Metalloenzyme Studies, University of Georgia, Athens, GA 30602, USA
    J Am Chem Soc 128:16566-78. 2006
    ....
  20. pmc Functional, structural, and spectroscopic characterization of a glutathione-ligated [2Fe-2S] cluster in poplar glutaredoxin C1
    Nicolas Rouhier
    Unité Mixte de Recherche 1136, Institut National de la Recherche Agronomique, Institut Fédératif de Recherche 110, Genomics, Ecology, Nancy University, BP 239, 54506 Vandoeuvre les Nancy Cedex, France
    Proc Natl Acad Sci U S A 104:7379-84. 2007
    ..In light of these results, the possible involvement of plant glutaredoxins in oxidative stress sensing or iron-sulfur biosynthesis is discussed with respect to their intracellular localization...
  21. ncbi request reprint Insights into the role of nucleotide-dependent conformational change in nitrogenase catalysis: Structural characterization of the nitrogenase Fe protein Leu127 deletion variant with bound MgATP
    Sanchayita Sen
    Department of Chemistry and Biochemistry, Montana State University, 108 Gaines Hall, Bozeman, MT 59717, USA
    J Inorg Biochem 100:1041-52. 2006
    ..This could be critical to the tight control of MgATP hydrolysis observed within the nitrogenase complex and may be important for maintaining unidirectional electron flow toward substrate reduction...
  22. ncbi request reprint Heterodisulfide reductase from Methanothermobacter marburgensis contains an active-site [4Fe-4S] cluster that is directly involved in mediating heterodisulfide reduction
    Evert C Duin
    Max Planck Institut für Terrestrische Mikrobiologie and Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, Karl von Frisch Strasse, D 35043, Marburg, Germany
    FEBS Lett 512:263-8. 2002
    ..The catalytic mechanism of HDR is discussed in light of the crystallographic and spectroscopic studies of the related chloroplast ferredoxin:thioredoxin reductase class of disulfide reductases...
  23. ncbi request reprint Spectroscopic evidence for site specific chemistry at a unique iron site of the [4Fe-4S] cluster in ferredoxin:thioredoxin reductase
    Guy N L Jameson
    Department of Physics, Emory University, Atlanta, Georgia 30322, USA
    J Am Chem Soc 125:1146-7. 2003
    ....
  24. ncbi request reprint Spectroscopic investigation of the nickel-containing porphinoid cofactor F(430). Comparison of the free cofactor in the (+)1, (+)2 and (+)3 oxidation states with the cofactor bound to methyl-coenzyme M reductase in the silent, red and ox forms
    Evert C Duin
    Department of Chemistry and Biochemistry, Auburn University, Auburn, AL 36849, USA
    J Biol Inorg Chem 9:563-76. 2004
    ..The reinterpretation of MCRox1 as a formal Ni(III) species rather than an Ni(I) species obviates the need to invoke a two-electron reduction of the F(430) macrocyclic ligand on reductive activation of MCRox1 to yield MCRred1...
  25. ncbi request reprint Iron-sulfur cluster assembly: NifU-directed activation of the nitrogenase Fe protein
    Patricia C Dos Santos
    Department of Biochemistry, Virginia Tech, Blacksburg, Virginia 24061, USA
    J Biol Chem 279:19705-11. 2004
    ..These in vivo experiments were supported by in vitro [Fe-S] cluster assembly and transfer experiments involving the activation of an apo-form of the nitrogenase Fe protein...
  26. ncbi request reprint A conformational mimic of the MgATP-bound "on state" of the nitrogenase iron protein
    Sanchayita Sen
    Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717, USA
    Biochemistry 43:1787-97. 2004
    ....
  27. ncbi request reprint Biotin sulfoxide reductase: Tryptophan 90 is required for efficient substrate utilization
    Veronica V Pollock
    Department of Biochemistry and Molecular Biology, College of Medicine, University of South Florida, Tampa, FL 33612, USA
    Arch Biochem Biophys 409:315-26. 2003
    ..These results strongly suggest that W90 in BSOR plays a critical role in catalysis by serving as a hydrogen bond donor to the oxo group on the Mo(VI) center...
  28. pmc Sulfur K-edge X-ray absorption spectroscopy and density functional theory calculations on superoxide reductase: role of the axial thiolate in reactivity
    Abhishek Dey
    Department of Chemistry, Stanford University, Stanford, California 94305, USA
    J Am Chem Soc 129:12418-31. 2007
    ..However, in contrast to FeSOD, the 5C FeIII site of SOR, which is more positively charged, is calculated to have a high affinity for binding a sixth anionic ligand, which would inhibit its SOD activity...