KENNETH ALLEN JOHNSON

Summary

Affiliation: University of Texas
Country: USA

Publications

  1. pmc A novel mechanism of selectivity against AZT by the human mitochondrial DNA polymerase
    Jeremiah W Hanes
    Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, The University of Texas, Austin, TX 78712, USA
    Nucleic Acids Res 35:6973-83. 2007
  2. doi request reprint A century of enzyme kinetic analysis, 1913 to 2013
    Kenneth A Johnson
    Institute for Cell and Molecular Biology, Department of Chemistry and Biochemistry, University of Texas at Austin, 2500 Speedway, MBB 3 122, Austin, TX 78735, USA
    FEBS Lett 587:2753-66. 2013
  3. doi request reprint FitSpace explorer: an algorithm to evaluate multidimensional parameter space in fitting kinetic data
    Kenneth A Johnson
    KinTek Corporation, Austin, TX 78735, USA
    Anal Biochem 387:30-41. 2009
  4. doi request reprint Global kinetic explorer: a new computer program for dynamic simulation and fitting of kinetic data
    Kenneth A Johnson
    KinTek Corporation, Austin, TX 78735, USA
    Anal Biochem 387:20-9. 2009
  5. pmc The original Michaelis constant: translation of the 1913 Michaelis-Menten paper
    Leonor Michaelis
    Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, The University of Texas, Austin, Texas 78735, United States
    Biochemistry 50:8264-9. 2011
  6. pmc Role of induced fit in enzyme specificity: a molecular forward/reverse switch
    Kenneth A Johnson
    Department of Chemistry and Biochemistry, Institute of Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA
    J Biol Chem 283:26297-301. 2008
  7. pmc The kinetic and chemical mechanism of high-fidelity DNA polymerases
    Kenneth A Johnson
    Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas, 2500 Speedway, Austin, TX 78712, USA
    Biochim Biophys Acta 1804:1041-8. 2010
  8. pmc High-cell density shake-flask expression and rapid purification of the large fragment of Thermus aquaticus DNA polymerase I using a new chemically and temperature inducible expression plasmid in Escherichia coli
    John W Brandis
    Institute for Cell and Molecular Biology, 2500 Speedway, University of Texas at Austin, Austin, TX 78712, USA
    Protein Expr Purif 63:120-7. 2009
  9. ncbi request reprint Novel mechanism of inhibition of HIV-1 reverse transcriptase by a new non-nucleoside analog, KM-1
    Louise Z Wang
    Department of Chemistry and Biochemistry, Institute of Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA
    J Biol Chem 279:38424-32. 2004
  10. doi request reprint Fitting enzyme kinetic data with KinTek Global Kinetic Explorer
    Kenneth A Johnson
    Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas, Austin, Texas, USA
    Methods Enzymol 467:601-26. 2009

Research Grants

  1. MECHANISM AND FIDELITY OF DNA REPLICATION
    Kenneth Johnson; Fiscal Year: 2006
  2. MECHANISM AND FIDELITY OF DNA REPLICATION
    Kenneth Johnson; Fiscal Year: 2003
  3. KINETIC ANALYSIS OF MICROTUBULE-DEPENDENT ATPASES
    Kenneth Johnson; Fiscal Year: 2003
  4. Elementary Steps in DNA Polymerization
    Kenneth Johnson; Fiscal Year: 2007
  5. Mechanism of mitochondrial DNA replication
    KENNETH ALLEN JOHNSON; Fiscal Year: 2010
  6. Nucleotide selectivity and drug resistance by HIV reverse transcriptase
    KENNETH ALLEN JOHNSON; Fiscal Year: 2010

Collaborators

  • ERIC KOOL
  • Ian Molineux
  • John W Brandis
  • Borries Demeler
  • CHRISTIAN WHITMAN
  • Hung wen Liu
  • Jie Zhang
  • Maurizio Brunori
  • Andrea Bellelli
  • Pär Nordlund
  • K S Anderson
  • Alexander Wong
  • Carmelinda Savino
  • B Stec
  • Jeremiah W Hanes
  • Harold R Lee
  • Muse Oke
  • James H Naismith
  • Stephen A McMahon
  • Young Sam Lee
  • Yu Chih Tsai
  • Malcolm F White
  • Scott D Auerbach
  • Lester G Carter
  • Mark Dorward
  • Leonor Michaelis
  • Joy Y Feng
  • Matthew W Kellinger
  • Dipanwita Batabyal
  • Y Whitney Yin
  • Eisuke Murakami
  • Harold Lee
  • Brooklyn A Robertson
  • Zhinan Jin
  • Jodi D Richards
  • Sandra A Helquist
  • Jason D Fowler
  • Catherine H Botting
  • Lester Carter
  • Alan Cooper
  • Martin A Walsh
  • Beverley A Yard
  • Ian M Overton
  • Aishah A Latiff
  • Jaldappagari Seetharamappa
  • Carlos Bloch
  • Malcolm W Kennedy
  • Geoffrey J Barton
  • Rosalind Tan Yan Ching
  • Garry L Taylor
  • Shashi Bhushan
  • B Martin Hallberg
  • Craig A Bingman
  • Brian G Fox
  • Won Bae Jeon
  • George N Phillips
  • Simon T M Allard
  • Louise Z Wang
  • Francesco Angelucci
  • Jeremiah Hanes
  • Kimberly A Stieglitz
  • Maud Leonora Menten
  • Roger S Goody
  • Sujin Lee
  • Jessica L McKenzie
  • Gottfried K Schroeder
  • Anne Marie McRobbie
  • Zucai Suo
  • Jessica A Brown
  • Stephen McMahon
  • Daphné Puech
  • Peter J Coote
  • Dominic J Campopiano
  • C A Johannes van Niekirk
  • Shirley Graham
  • Michal Zawadzki
  • Deborah S Parris
  • Yali Zhu
  • David M Thal
  • Heidi Erlandsen
  • Elzbieta Glaser
  • Stephen Douthwaite
  • Albert E Beuscher
  • Therese Eneqvist
  • Niels Møller Andersen
  • Ulrika B Ericsson
  • Todd Kimball
  • Blake Buchan
  • David Aceti
  • Donna Troestler

Detail Information

Publications47

  1. pmc A novel mechanism of selectivity against AZT by the human mitochondrial DNA polymerase
    Jeremiah W Hanes
    Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, The University of Texas, Austin, TX 78712, USA
    Nucleic Acids Res 35:6973-83. 2007
    ..Other azido-nucleotides (AZG, AZC and AZA) and 8-oxo-7,8-dihydroguanosine-5'-triphosphate (8-oxo-dGTP) show this same phenomena...
  2. doi request reprint A century of enzyme kinetic analysis, 1913 to 2013
    Kenneth A Johnson
    Institute for Cell and Molecular Biology, Department of Chemistry and Biochemistry, University of Texas at Austin, 2500 Speedway, MBB 3 122, Austin, TX 78735, USA
    FEBS Lett 587:2753-66. 2013
    ..Finally, several illustrations of modern transient state kinetic methods of analysis are shown which enable the elucidation of reactions occurring at the active sites of enzymes in order to relate structure and function...
  3. doi request reprint FitSpace explorer: an algorithm to evaluate multidimensional parameter space in fitting kinetic data
    Kenneth A Johnson
    KinTek Corporation, Austin, TX 78735, USA
    Anal Biochem 387:30-41. 2009
    ..In contrast, analysis of alanine racemase clearly refutes claims that global analysis of progress curves can be used to extract the free energy profiles of enzyme-catalyzed reactions...
  4. doi request reprint Global kinetic explorer: a new computer program for dynamic simulation and fitting of kinetic data
    Kenneth A Johnson
    KinTek Corporation, Austin, TX 78735, USA
    Anal Biochem 387:20-9. 2009
    ..This feature is critical to avoid overly complex models that are not supported by the data...
  5. pmc The original Michaelis constant: translation of the 1913 Michaelis-Menten paper
    Leonor Michaelis
    Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, The University of Texas, Austin, Texas 78735, United States
    Biochemistry 50:8264-9. 2011
    ..That constant was not the Michaelis constant, but rather V(max)/K(m), the specificity constant times the enzyme concentration (k(cat)/K(m) × E(0))...
  6. pmc Role of induced fit in enzyme specificity: a molecular forward/reverse switch
    Kenneth A Johnson
    Department of Chemistry and Biochemistry, Institute of Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA
    J Biol Chem 283:26297-301. 2008
    ..A conformational switch may also favor release rather than reverse reaction of the product...
  7. pmc The kinetic and chemical mechanism of high-fidelity DNA polymerases
    Kenneth A Johnson
    Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas, 2500 Speedway, Austin, TX 78712, USA
    Biochim Biophys Acta 1804:1041-8. 2010
    ....
  8. pmc High-cell density shake-flask expression and rapid purification of the large fragment of Thermus aquaticus DNA polymerase I using a new chemically and temperature inducible expression plasmid in Escherichia coli
    John W Brandis
    Institute for Cell and Molecular Biology, 2500 Speedway, University of Texas at Austin, Austin, TX 78712, USA
    Protein Expr Purif 63:120-7. 2009
    ..8-L baffle-bottomed shake-flasks each holding 1.5L of culture for a final yield of approximately 33 mg per liter or 3mg of purified enzyme per gram of cells wet weight...
  9. ncbi request reprint Novel mechanism of inhibition of HIV-1 reverse transcriptase by a new non-nucleoside analog, KM-1
    Louise Z Wang
    Department of Chemistry and Biochemistry, Institute of Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA
    J Biol Chem 279:38424-32. 2004
    ..KM-1 can bind to RT in both the absence and presence of DNA but weakens the affinity for DNA 140-fold so that it favors DNA dissociation. The data suggest that KM-1 distorts RT conformation and misaligns DNA at the active site...
  10. doi request reprint Fitting enzyme kinetic data with KinTek Global Kinetic Explorer
    Kenneth A Johnson
    Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas, Austin, Texas, USA
    Methods Enzymol 467:601-26. 2009
    ..These concepts are illustrated by example in fitting full progress curve kinetics and in fitting the time dependence of slow-onset inhibition...
  11. pmc Nucleotide-dependent conformational change governs specificity and analog discrimination by HIV reverse transcriptase
    Matthew W Kellinger
    Institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78712, USA
    Proc Natl Acad Sci U S A 107:7734-9. 2010
    ..Discrimination against 3TC-TP is based on the slower rate of incorporation due to misalignment of the substrate and/or catalytic residues...
  12. ncbi request reprint Incorporation and replication of 8-oxo-deoxyguanosine by the human mitochondrial DNA polymerase
    Jeremiah W Hanes
    Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, The University of Texas, Austin, Texas 78712, USA
    J Biol Chem 281:36241-8. 2006
    ..These reactions are likely to play important roles in oxidative stress in mitochondria related to aging and as compounded by nucleoside analogs used to treat human immunodeficiency virus infections...
  13. pmc Role of histidine 932 of the human mitochondrial DNA polymerase in nucleotide discrimination and inherited disease
    Dipanwita Batabyal
    Department of Chemistry and Biochemistry, Institute of Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA
    J Biol Chem 285:34191-201. 2010
    ..His-932 is thought to contact the β-phosphate of the incoming nucleotide, so it is perhaps surprising that H932Y appears to slow rather than accelerate pyrophosphate release...
  14. ncbi request reprint A new paradigm for DNA polymerase specificity
    Yu Chih Tsai
    Institute for Cellular and Molecular Biology, Department of Chemistry and Biochemistry, University of Texas, 2500 Speedway, Austin, Texas 78712, USA
    Biochemistry 45:9675-87. 2006
    ....
  15. ncbi request reprint Analysis of single nucleotide incorporation reactions by capillary electrophoresis
    Jeremiah W Hanes
    Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX 78712, USA
    Anal Biochem 340:35-40. 2005
    ..However, we also show that only select probes can be used, presumably due to unfavorable interactions between Pol gamma and certain 5' labels...
  16. pmc Site-specific labeling of T7 DNA polymerase with a conformationally sensitive fluorophore and its use in detecting single-nucleotide polymorphisms
    Yu Chih Tsai
    Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78712, USA
    Anal Biochem 384:136-44. 2009
    ..Finally, we show that the labeled enzyme can be used to identify single-nucleotide mutations in a procedure that could be automated...
  17. ncbi request reprint Enzymatic therapeutic index of acyclovir. Viral versus human polymerase gamma specificity
    Jeremiah W Hanes
    Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, The University of Texas, Austin, Texas 78712, USA
    J Biol Chem 282:25159-67. 2007
    ..Mitochondrial toxicity is relatively low because acyclovir is activated only in infected cells by the promiscuous viral thymidine kinase and otherwise, mitochondrial toxicity would accumulate during long term treatment...
  18. ncbi request reprint Fidelity of the human mitochondrial DNA polymerase
    Harold R Lee
    Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA
    J Biol Chem 281:36236-40. 2006
    ..We calculate an average fidelity of 1 error in 440,000 nucleotides...
  19. pmc Pre-steady-state kinetic analysis of cis-3-chloroacrylic acid dehalogenase: analysis and implications
    Brooklyn A Robertson
    Department of Chemistry and Biochemistry, College of Pharmacy, University of Texas, Austin, Texas 78712, USA
    Biochemistry 48:11737-44. 2009
    ..In the unliganded form, the same loop points away from the active site cavity. Similarly, substrate binding may cause this loop to close down on the active site and sequester the reaction from the external environment...
  20. ncbi request reprint Fidelity and processivity of reverse transcription by the human mitochondrial DNA polymerase
    Harold R Lee
    Department of Chemistry and Biochemistry, Institute of Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA
    J Biol Chem 282:31982-9. 2007
    ..The biological and biotechnological significance of these results are discussed...
  21. pmc Base pair hydrogen bonds are essential for proofreading selectivity by the human mitochondrial DNA polymerase
    Harold R Lee
    Department of Chemistry and Biochemistry, Institute of Cellular and Molecular Biology, University of Texas, Austin, TX 78712, USA
    J Biol Chem 283:14411-6. 2008
    ..The enzyme may use the alignment and prevention of fraying provided by proper hydrogen bonding and minor groove hydrogen bonding interactions as critical criteria for correct base pair recognition...
  22. pmc Importance of hydrogen bonding for efficiency and specificity of the human mitochondrial DNA polymerase
    Harold R Lee
    Department of Chemistry and Biochemistry, Institute of Cellular and Molecular Biology, University of Texas, Austin, TX 78712, USA
    J Biol Chem 283:14402-10. 2008
    ..Base pair hydrogen bonding contributes at least a third of the energy underlying nucleoside incorporation efficiency and specificity...
  23. ncbi request reprint Kinetic effects of kinesin switch I and switch II mutations
    Scott D Auerbach
    Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA
    J Biol Chem 280:37061-8. 2005
    ..These results further clarify the pathway of coupling of ATP hydrolysis to force production...
  24. ncbi request reprint Alternating site ATPase pathway of rat conventional kinesin
    Scott D Auerbach
    Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA
    J Biol Chem 280:37048-60. 2005
    ..These studies provide a baseline set of kinetic parameters against which future studies of rat kinesin mutants may be evaluated and directly correlated with the structure of the dimeric kinesin...
  25. pmc A single mutation in human mitochondrial DNA polymerase Pol gammaA affects both polymerization and proofreading activities of only the holoenzyme
    Young Sam Lee
    Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA
    J Biol Chem 285:28105-16. 2010
    ..This study provides a molecular basis for the disease symptoms exhibited by patients carrying those substitutions...
  26. ncbi request reprint Study of 1-deoxy-D-xylulose-5-phosphate reductoisomerase: synthesis and evaluation of fluorinated substrate analogues
    Alexander Wong
    Division of Medicinal Chemistry, College of Pharmacy and Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712, USA
    Org Lett 6:3625-8. 2004
    ..It was found that the 1-fluoro-DXP is a poor substrate, while both 3- and 4-fluoro-DXP behave as noncompetitive inhibitors...
  27. ncbi request reprint Toxicity of nucleoside analogues used to treat AIDS and the selectivity of the mitochondrial DNA polymerase
    Harold Lee
    Institute for Cell and Molecular Biology, Department of Chemistry and Biochemistry, University of Texas at Austin, 78712, USA
    Biochemistry 42:14711-9. 2003
    ..We will also discuss the promise and underlying challenges for the development of new analogues with lower toxicity...
  28. pmc Each monomer of the dimeric accessory protein for human mitochondrial DNA polymerase has a distinct role in conferring processivity
    Young Sam Lee
    Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA
    J Biol Chem 285:1490-9. 2010
    ..By duplicating the monomeric pol gammaB of lower eukaryotes, the dimeric mammalian proteins confer additional processivity to the holoenzyme polymerase...
  29. pmc Exonuclease removal of dideoxycytidine (zalcitabine) by the human mitochondrial DNA polymerase
    Jeremiah W Hanes
    Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, The University of Texas, Austin, TX 78712, USA
    Antimicrob Agents Chemother 52:253-8. 2008
    ....
  30. pmc Real-time measurement of pyrophosphate release kinetics
    Jeremiah W Hanes
    Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, The University of Texas, Austin, TX 78712, USA
    Anal Biochem 372:125-7. 2008
  31. pmc Relationship between antiviral activity and host toxicity: comparison of the incorporation efficiencies of 2',3'-dideoxy-5-fluoro-3'-thiacytidine-triphosphate analogs by human immunodeficiency virus type 1 reverse transcriptase and human mitochondrial DNA
    Joy Y Feng
    Gilead Sciences, Durham, North Carolina 27707, USA
    Antimicrob Agents Chemother 48:1300-6. 2004
    ..6-fold slower than the excision of dCMP. These results demonstrate that there are clear differences between HIV-1 RT and polymerase gamma in terms of preferences for substrate structure...
  32. pmc Kinetic investigation of the inhibitory effect of gemcitabine on DNA polymerization catalyzed by human mitochondrial DNA polymerase
    Jason D Fowler
    Department of Biochemistry, Ohio State Biochemistry Program, Ohio State University, Columbus, OH 43210, USA
    J Biol Chem 283:15339-48. 2008
    ..Thus, our pre-steady state kinetic studies provide direct evidence demonstrating the inhibitory effect of gemcitabine on the activity of human mitochondrial DNA polymerase...
  33. ncbi request reprint Crystal structure of a dual activity IMPase/FBPase (AF2372) from Archaeoglobus fulgidus. The story of a mobile loop
    Kimberly A Stieglitz
    Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02467, USA
    J Biol Chem 277:22863-74. 2002
    ..A direct correlation of the loop conformation with strength of Li(+) inhibition provides a useful system of classification for this extended family of enzymes...
  34. ncbi request reprint Characterization of novel reverse transcriptase and other RNA-associated catalytic activities by human DNA polymerase gamma: importance in mitochondrial DNA replication
    Eisuke Murakami
    Department of Pharmacology, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut 06520 8066, USA
    J Biol Chem 278:36403-9. 2003
    ..Our finding that Pol gamma also incorporates ribonucleotide triphosphates into a DNA primer offers a plausible enzymatic pathway for the origin of the RNA-containing mtDNA genome...
  35. ncbi request reprint Schistosoma mansoni fatty acid binding protein: specificity and functional control as revealed by crystallographic structure
    Francesco Angelucci
    Department of Biochemical Sciences A Rossi Fanelli, CNR Institute of Molecular Biology and Pathology and Istituto Pasteur Fondazione Cenci Bolognetti, University of Rome La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy
    Biochemistry 43:13000-11. 2004
    ..The binding rate constant of ligands is controlled by a slow pH dependent conformational change, which we propose to have physiological relevance...
  36. ncbi request reprint Crystal structure of the 28 kDa glutathione S-transferase from Schistosoma haematobium
    Kenneth A Johnson
    Department of Biochemical Sciences and Istituto Pasteur Fondazione Cenci Bolognetti, University of Rome La Sapienza, Rome, Italy
    Biochemistry 42:10084-94. 2003
    ..The relatively tight binding of GSH by Sh28GST explains the residually bound GSH in the crystal and supports a possible role of GSH as a tightly bound cofactor involved in the catalytic mechanism for prostaglandin D(2) synthase activity...
  37. ncbi request reprint Insights into DNA replication: the crystal structure of DNA polymerase B1 from the archaeon Sulfolobus solfataricus
    Carmelinda Savino
    Department of Biochemical Sciences and Consiglio Nazionale delle Ricerche, Institute of Molecular Biology and Pathology, University of Rome La Sapienza, P le A Moro 5, 00185 Roma, Italy
    Structure 12:2001-8. 2004
    ....
  38. pmc Unusual chromophore and cross-links in ranasmurfin: a blue protein from the foam nests of a tropical frog
    Muse Oke
    Centre for Biomolecular Science and The Scottish Structural Proteomics Facility, The University, St Andrews, UK
    Angew Chem Int Ed Engl 47:7853-6. 2008
  39. ncbi request reprint The structure of the RNA m5C methyltransferase YebU from Escherichia coli reveals a C-terminal RNA-recruiting PUA domain
    B Martin Hallberg
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, 17177 Stockholm, Sweden
    J Mol Biol 360:774-87. 2006
    ..The ribosomal protein interactions would explain why the assembled 30 S subunit, and not naked 16 S rRNA, is the preferred substrate for YebU...
  40. pmc Structure at 1.6 A resolution of the protein from gene locus At3g22680 from Arabidopsis thaliana
    Simon T M Allard
    Center for Eukaryotic Structural Genomics, Department of Biochemistry, University of Wisconsin Madison, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:647-50. 2005
    ..Protein At3g22680 shows no structural homology to any other known proteins and represents a new fold in protein conformation space...
  41. ncbi request reprint Proteolytic mechanism of a novel mitochondrial and chloroplastic PreP peptidasome
    Shashi Bhushan
    Department for Biochemistry and Biophysics, Arrhenius Laboratories for Natural Sciences, Stockholm University, S 106 91 Stockholm, Sweden
    Biol Chem 387:1087-90. 2006
    ..The overall results support the proposed mechanism...
  42. pmc Crystallization of Ranasmurfin, a blue-coloured protein from Polypedates leucomystax
    Stephen A McMahon
    Centre for Biomolecular Science and The Scottish Structural Proteomics Facility, The University of St Andrews Fife KY16 9RH, Scotland, UK
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:1124-6. 2006
    ..A fluorescence scan shows a peak at 9.676 keV, indicating that the protein binds zinc and suggesting a route for structure solution...
  43. ncbi request reprint The structure of serine palmitoyltransferase; gateway to sphingolipid biosynthesis
    Beverley A Yard
    School of Chemistry, EaStCHEM, The University of Edinburgh, West Mains Road, Edinburgh, EH9 3JJ, Scotland, UK
    J Mol Biol 370:870-86. 2007
    ..Our models provide an understanding of how these mutations may affect the activity of the enzyme...
  44. pmc Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase
    Jaldappagari Seetharamappa
    Scottish Structural Facility and Centre for Biomolecular Sciences, The University, St Andrews KY16 9ST, Scotland
    Acta Crystallogr Sect F Struct Biol Cryst Commun 63:452-6. 2007
    ..6, b = 91.3, c = 106.0 A, alpha = beta = gamma = 90 degrees. Analysis of the systematic absences along the three principal axes indicated the space group to be P2(1)2(1)2(1). A complete data set was collected to 2.5 A resolution...
  45. pmc Structure of the DNA repair helicase hel308 reveals DNA binding and autoinhibitory domains
    Jodi D Richards
    Centre for Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, Scotland
    J Biol Chem 283:5118-26. 2008
    ..These data suggest that one function of the enzyme may be in the removal of bound proteins at stalled replication forks and recombination intermediates...
  46. ncbi request reprint X-ray structure of Arabidopsis At1g77680, 12-oxophytodienoate reductase isoform 1
    Brian G Fox
    University of Wisconsin Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, 53706, USA
    Proteins 61:206-8. 2005
  47. pmc The closed structure of presequence protease PreP forms a unique 10,000 Angstroms3 chamber for proteolysis
    Kenneth A Johnson
    Department of Biochemistry and Biophysics, Arrhenius Laboratories for Natural Sciences, Stockholm University, Sweden
    EMBO J 25:1977-86. 2006
    ..The manner in which substrates are processed inside the chamber is reminiscent of the proteasome; therefore, we refer to this protein as a peptidasome...

Research Grants23

  1. MECHANISM AND FIDELITY OF DNA REPLICATION
    Kenneth Johnson; Fiscal Year: 2006
    ..abstract_text> ..
  2. MECHANISM AND FIDELITY OF DNA REPLICATION
    Kenneth Johnson; Fiscal Year: 2003
    ..The mechanism and fidelity of the mitochondrial polymerase will be established by kinetic measurements. ..
  3. KINETIC ANALYSIS OF MICROTUBULE-DEPENDENT ATPASES
    Kenneth Johnson; Fiscal Year: 2003
    ..The combination of approaches outlined here will provide rigorous and direct information to define the structural and mechanistic basis for force production by kinesin. ..
  4. Elementary Steps in DNA Polymerization
    Kenneth Johnson; Fiscal Year: 2007
    ..abstract_text> ..
  5. Mechanism of mitochondrial DNA replication
    KENNETH ALLEN JOHNSON; Fiscal Year: 2010
    ....
  6. Nucleotide selectivity and drug resistance by HIV reverse transcriptase
    KENNETH ALLEN JOHNSON; Fiscal Year: 2010
    ....