James R Johnson

Summary

Affiliation: University of Minnesota
Country: USA

Publications

  1. doi request reprint Molecular Analysis of Escherichia coli from retail meats (2002-2004) from the United States National Antimicrobial Resistance Monitoring System
    James R Johnson
    Department of Medicine, University of Minnesota, Veterans Affairs Medical Center, Minneapolis, Minnesota 55417, USA
    Clin Infect Dis 49:195-201. 2009
  2. pmc Comparison of extraintestinal pathogenic Escherichia coli strains from human and avian sources reveals a mixed subset representing potential zoonotic pathogens
    Timothy J Johnson
    Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, Minnesota 551081, USA
    Appl Environ Microbiol 74:7043-50. 2008
  3. pmc Escherichia coil cluster evaluation
    James R Johnson
    Emerg Infect Dis 13:520. 2007
  4. pmc Extended-Spectrum B-Lactamase-producing Escherichia coli in Neonatal Care Unit
    James R Johnson
    Veterans Affairs Medical Center, Minneapolis, Minnesota, USA
    Emerg Infect Dis 17:153-1154. 2011
  5. pmc Abrupt emergence of a single dominant multidrug-resistant strain of Escherichia coli
    James R Johnson
    Veterans Affairs Medical Center and University of Minnesota, Minneapolis, USA
    J Infect Dis 207:919-28. 2013
  6. pmc In vitro comparison of nitrofurazone- and silver alloy-coated foley catheters for contact-dependent and diffusible inhibition of urinary tract infection-associated microorganisms
    James R Johnson
    VA Medical Center and University of Minnesota, Minneapolis, Minnesota, USA
    Antimicrob Agents Chemother 56:4969-72. 2012
  7. pmc Molecular epidemiological analysis of Escherichia coli sequence type ST131 (O25:H4) and blaCTX-M-15 among extended-spectrum-β-lactamase-producing E. coli from the United States, 2000 to 2009
    James R Johnson
    Veterans Affairs Medical Center, Minneapolis, Minnesota, USA
    Antimicrob Agents Chemother 56:2364-70. 2012
  8. pmc Comparison of Escherichia coli ST131 pulsotypes, by epidemiologic traits, 1967-2009
    James R Johnson
    Veterans Affairs Medical Center, Minneapolis, Minnesota 55417, USA
    Emerg Infect Dis 18:598-607. 2012
  9. doi request reprint Global distribution and epidemiologic associations of Escherichia coli clonal group A, 1998-2007
    James R Johnson
    Veterans Affairs Medical Center, Minneapolis, Minnesota 55417, USA
    Emerg Infect Dis 17:2001-9. 2011
  10. pmc Virulence of Escherichia coli clinical isolates in a murine sepsis model in relation to sequence type ST131 status, fluoroquinolone resistance, and virulence genotype
    James R Johnson
    Veterans Affairs Medical Center, Minneapolis, Minnesota, USA
    Infect Immun 80:1554-62. 2012

Research Grants

  1. Resistant E. coli in Humans and Poultry
    James Johnson; Fiscal Year: 2004
  2. Resistant E.coli in Humans and Poultry
    James Johnson; Fiscal Year: 2005
  3. Infectious Disease Training in Clinical Investigation
    James Johnson; Fiscal Year: 2007

Detail Information

Publications102 found, 100 shown here

  1. doi request reprint Molecular Analysis of Escherichia coli from retail meats (2002-2004) from the United States National Antimicrobial Resistance Monitoring System
    James R Johnson
    Department of Medicine, University of Minnesota, Veterans Affairs Medical Center, Minneapolis, Minnesota 55417, USA
    Clin Infect Dis 49:195-201. 2009
    ..The origins and virulence potential of meat product-associated Escherichia coli are undefined...
  2. pmc Comparison of extraintestinal pathogenic Escherichia coli strains from human and avian sources reveals a mixed subset representing potential zoonotic pathogens
    Timothy J Johnson
    Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, Minnesota 551081, USA
    Appl Environ Microbiol 74:7043-50. 2008
    ..Also, the widespread occurrence of plasmids among NMEC strains and members of the mixed cluster suggests that plasmid-mediated virulence in these pathotypes warrants further attention...
  3. pmc Escherichia coil cluster evaluation
    James R Johnson
    Emerg Infect Dis 13:520. 2007
  4. pmc Extended-Spectrum B-Lactamase-producing Escherichia coli in Neonatal Care Unit
    James R Johnson
    Veterans Affairs Medical Center, Minneapolis, Minnesota, USA
    Emerg Infect Dis 17:153-1154. 2011
    ..This finding advances our understanding of how extended-spectrum B-lactamase-positive (and, by extension, other antimicrobial drug-resistant or virulent strains) E. coli can spread within the community...
  5. pmc Abrupt emergence of a single dominant multidrug-resistant strain of Escherichia coli
    James R Johnson
    Veterans Affairs Medical Center and University of Minnesota, Minneapolis, USA
    J Infect Dis 207:919-28. 2013
    ..Fluoroquinolone-resistant Escherichia coli are increasingly prevalent. Their clonal origins--potentially critical for control efforts--remain undefined...
  6. pmc In vitro comparison of nitrofurazone- and silver alloy-coated foley catheters for contact-dependent and diffusible inhibition of urinary tract infection-associated microorganisms
    James R Johnson
    VA Medical Center and University of Minnesota, Minneapolis, Minnesota, USA
    Antimicrob Agents Chemother 56:4969-72. 2012
    ..Although inhibition waned with catheter preincubation in saline, some organisms were inhibited even after a 48-h catheter preincubation, especially by the nitrofurazone-coated catheter...
  7. pmc Molecular epidemiological analysis of Escherichia coli sequence type ST131 (O25:H4) and blaCTX-M-15 among extended-spectrum-β-lactamase-producing E. coli from the United States, 2000 to 2009
    James R Johnson
    Veterans Affairs Medical Center, Minneapolis, Minnesota, USA
    Antimicrob Agents Chemother 56:2364-70. 2012
    ..coli strains in the United States, among children and adults alike. Enhanced virulence and antimicrobial resistance have likely promoted the epidemiological success of these emerging public health threats...
  8. pmc Comparison of Escherichia coli ST131 pulsotypes, by epidemiologic traits, 1967-2009
    James R Johnson
    Veterans Affairs Medical Center, Minneapolis, Minnesota 55417, USA
    Emerg Infect Dis 18:598-607. 2012
    ..coli ST131 have recently emerged and diffused extensively among locales while maintaining a comparatively restricted host/source range. Identification of factors contributing to this behavior of ST131 could help protect public health...
  9. doi request reprint Global distribution and epidemiologic associations of Escherichia coli clonal group A, 1998-2007
    James R Johnson
    Veterans Affairs Medical Center, Minneapolis, Minnesota 55417, USA
    Emerg Infect Dis 17:2001-9. 2011
    ..The stable prevalence of CGA over time suggests full emergence by the late 1990s, followed by variable endemicity worldwide as an antimicrobial drug-resistant public health threat...
  10. pmc Virulence of Escherichia coli clinical isolates in a murine sepsis model in relation to sequence type ST131 status, fluoroquinolone resistance, and virulence genotype
    James R Johnson
    Veterans Affairs Medical Center, Minneapolis, Minnesota, USA
    Infect Immun 80:1554-62. 2012
    ..Additionally, the extensive within-ST virulence diversity suggests an opportunity to compare closely related strains to identify the responsible genetic determinants...
  11. pmc Effect of inactivation of the global oxidative stress regulator oxyR on the colonization ability of Escherichia coli O1:K1:H7 in a mouse model of ascending urinary tract infection
    James R Johnson
    University of Minnesota Department of Medicine, Infectious Diseases 111F, Minneapolis VA Medical Center, 1 Veterans Drive, Minneapolis, MN 55417, USA
    Infect Immun 74:461-8. 2006
    ..coli. They also suggest that neutrophil phagocyte oxidase is not critical for defense against E. coli UTI and that the major oxidative stresses against which OxyR protects E. coli within the host milieu are not phagocyte derived...
  12. ncbi request reprint Systematic review: antimicrobial urinary catheters to prevent catheter-associated urinary tract infection in hospitalized patients
    James R Johnson
    Veterans Affairs Medical Center and University of Minnesota, Minneapolis, Minnesota 55417, USA
    Ann Intern Med 144:116-26. 2006
    ..The efficacy of antimicrobial urinary catheters in hospitalized patients is poorly defined...
  13. pmc Bacterial characteristics in relation to clinical source of Escherichia coli isolates from women with acute cystitis or pyelonephritis and uninfected women
    James R Johnson
    Infectious Diseases 111F, Minneapolis VA Medical Center, 1 Veterans Drive, Minneapolis, MN 55417, USA
    J Clin Microbiol 43:6064-72. 2005
    ..These findings identify potential vaccine targets, suggest that urovirulence is multiply determined, and confirm the urovirulence of specific E. coli clonal groups, including recently recognized CGA...
  14. ncbi request reprint In vitro activity of available antimicrobial coated Foley catheters against Escherichia coli, including strains resistant to extended spectrum cephalosporins
    James R Johnson
    Veterans Affairs Medical Center and University of Minnesota, Minneapolis, Minnesota 55417, USA
    J Urol 184:2572-7. 2010
    ..We determined the activity, comparative potency and effect durability of commercially available, antimicrobial coated Foley catheters against Escherichia coli isolates, including extended spectrum cephalosporin resistant strains...
  15. pmc Spontaneous conversion to quinolone and fluoroquinolone resistance among wild-type Escherichia coli isolates in relation to phylogenetic background and virulence genotype
    James R Johnson
    Medical Service, VA Medical Center, Minneapolis, Minnesota 55417, USA
    Antimicrob Agents Chemother 49:4739-44. 2005
    ..A more likely explanation is the importation of resistant strains from an as-yet-undefined low-virulence external selection reservoir...
  16. ncbi request reprint Molecular epidemiology of extraintestinal pathogenic (uropathogenic) Escherichia coli
    James R Johnson
    Mucosal and Vaccine Research Center, Infectious Diseases 111F, VA Medical Center, 1 Veterans Drive, Minneapolis, MN 55417, USA
    Int J Med Microbiol 295:383-404. 2005
    ..Published by Elsevier GmbH...
  17. pmc Phylogenetic and pathotypic comparison of concurrent urine and rectal Escherichia coli isolates from men with febrile urinary tract infection
    James R Johnson
    Mucosal and Vaccine Research Center, Minneapolis VA Medical Center, 1 Veterans Drive, Minneapolis, MN 55417, USA
    J Clin Microbiol 43:3895-900. 2005
    ..They also identify specific bacterial traits as potential targets for anti-FUTI interventions...
  18. ncbi request reprint Contamination of retail foods, particularly turkey, from community markets (Minnesota, 1999-2000) with antimicrobial-resistant and extraintestinal pathogenic Escherichia coli
    James R Johnson
    Mucosal and Vaccine Research Center, Minneapolis VA Medical Center, Minneapolis, Minnesota 55417, USA
    Foodborne Pathog Dis 2:38-49. 2005
    ....
  19. ncbi request reprint Virulence characteristics and phylogenetic background of multidrug-resistant and antimicrobial-susceptible clinical isolates of Escherichia coli from across the United States, 2000-2001
    James R Johnson
    Mucosal and Vaccine Research Center, Veterans Affairs Medical Center, and Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55417, USA
    J Infect Dis 190:1739-44. 2004
    ..Increases in antimicrobial resistance in Escherichia coli have been paralleled by an increasing incidence of E. coli sepsis, suggesting a possible link between resistance and virulence...
  20. ncbi request reprint Escherichia coli colonization patterns among human household members and pets, with attention to acute urinary tract infection
    James R Johnson
    Mucosal and Vaccine Research Center, Minneapolis Veterans Affairs Medical Center, Minnesota, Minneapolis 55417, USA
    J Infect Dis 197:218-24. 2008
    ..Within-household transmission of Escherichia coli may promote urinary tract infection (UTI) but is poorly understood...
  21. ncbi request reprint Similarity between human and chicken Escherichia coli isolates in relation to ciprofloxacin resistance status
    James R Johnson
    Mucosal and Vaccine Research Center, University of Minnesota, Minneapolis, USA
    J Infect Dis 194:71-8. 2006
    ..The food supply is suspected to be a source of fluoroquinolone-resistant Escherichia coli that cause disease in humans, but supporting molecular data are lacking...
  22. pmc Molecular epidemiology and phylogenetic distribution of the Escherichia coli pks genomic island
    James R Johnson
    Medical Service, Veterans Affairs Medical Center, Minneapolis, Minnesota 55417, USA
    J Clin Microbiol 46:3906-11. 2008
    ..This extends previous work regarding the pks island and supports investigation of the colibactin system as a potential therapeutic target...
  23. ncbi request reprint Bacterial characteristics as predictors of posttherapy recurrent bacteriuria among children with acute uncomplicated cystitis caused by Escherichia coli
    James R Johnson
    Mucosal and Vaccine Research Center, Minneapolis Veterans Affairs Medical Center, Minneapolis, MN, USA
    Pediatr Infect Dis J 26:1151-3. 2007
    ..Rapid testing conceivably could facilitate rational selection of treatment duration for pediatric cystitis. Certain traits might represent good targets for preventive interventions...
  24. pmc Virulence genotypes and phylogenetic background of Escherichia coli serogroup O6 isolates from humans, dogs, and cats
    James R Johnson
    Mucosal and Vaccine Research Center, Veterans Affairs Medical Center, and Department of Medicine, University of Minnesota, Minneapolis, Minnesota, USA
    J Clin Microbiol 46:417-22. 2008
    ..coli clones. In conclusion, the close similarity in the genomic backbone and virulence genotype between certain human- and animal-source E. coli isolates within serogroup O6 supports the hypothesis of zoonotic potential...
  25. pmc Multiple-host sharing, long-term persistence, and virulence of Escherichia coli clones from human and animal household members
    James R Johnson
    Departments of Medicine and Psychiatry, Veterans Affairs Medical Center, University of Minnesota, Minneapolis, Minnesota 55417, USA
    J Clin Microbiol 46:4078-82. 2008
    ..Virulence genes, group B2, and having caused cystitis (in the mother or dog) corresponded to colonization endpoints (number of samples, colonies, hosts, and dates). The dog's cystitis clone was the most extensively recovered clone...
  26. pmc Antimicrobial drug-resistant Escherichia coli from humans and poultry products, Minnesota and Wisconsin, 2002-2004
    James R Johnson
    Minneapolis Veterans Affairs Medical Center, Minneapolis, Minnesota 55417, USA
    Emerg Infect Dis 13:838-46. 2007
    ..Many drug-resistant human fecal E. coli isolates may originate from poultry, whereas drug-resistant poultry source E. coli isolates likely originate from susceptible poultry-source precursors...
  27. pmc Epidemic clonal groups of Escherichia coli as a cause of antimicrobial-resistant urinary tract infections in Canada, 2002 to 2004
    James R Johnson
    Infectious Diseases 111F, Minneapolis VA Medical Center, 1 Veterans Drive, Minneapolis, MN 55417, USA
    Antimicrob Agents Chemother 53:2733-9. 2009
    ..coli ST131 being the major contributors. These clonal groups appear to be more virulent than comparably resistant isolates, possibly contributing to their success as emerging multi-drug-resistant pathogens...
  28. pmc Sharing of Escherichia coli sequence type ST131 and other multidrug-resistant and Urovirulent E. coli strains among dogs and cats within a household
    James R Johnson
    Infectious Diseases 111F, Veterans Affairs Medical Center, 1 Veterans Drive, Minneapolis, MN 55417, USA
    J Clin Microbiol 47:3721-5. 2009
    ..Of the four other colonizing strains identified, two were MDR and two colonized multiple hosts. The ST131 strain uniquely exhibited high resistance and virulence scores...
  29. ncbi request reprint Sharing of virulent Escherichia coli clones among household members of a woman with acute cystitis
    James R Johnson
    Mucosal and Vaccine Research Center, Veterans Affairs Medical Center, Minneapolis, MN 55417, USA
    Clin Infect Dis 43:e101-8. 2006
    ..Within-household transmission of extraintestinal pathogenic Escherichia coli (ExPEC) may contribute to the pathogenesis of urinary tract infection (UTI), but this is poorly understood...
  30. pmc Escherichia coli sequence type ST131 as an emerging fluoroquinolone-resistant uropathogen among renal transplant recipients
    James R Johnson
    Infectious Diseases 111F, VA Medical Center, 1 Veterans Drive, Minneapolis, MN 55417, USA
    Antimicrob Agents Chemother 54:546-50. 2010
    ..ST131 emerged significantly during the study period. These findings suggest that E. coli ST131 may constitute an important new multidrug-resistant threat to renal transplant recipients...
  31. doi request reprint Within-household sharing of a fluoroquinolone-resistant Escherichia coli sequence type ST131 strain causing pediatric osteoarticular infection
    James R Johnson
    Infectious Diseases Section, Veterans Affairs Medical Center, Minneapolis, MN 55417, USA
    Pediatr Infect Dis J 29:473-5. 2010
    ..coli strain with the father. Within-household transmission can contribute to the dissemination of the emerging, multidrug-resistant ST131 clonal group, which has evident invasive potential for otherwise-healthy children...
  32. ncbi request reprint Experimental mouse lethality of Escherichia coli isolates, in relation to accessory traits, phylogenetic group, and ecological source
    James R Johnson
    Mucosal and Vaccine Research Center, VA Medical Center, University of Minnesota, Minneapolis, MN 55417, USA
    J Infect Dis 194:1141-50. 2006
    ..Whether accessory traits, phylogenetic background, or ecological source best predicts extraintestinal virulence within Escherichia coli is undefined...
  33. doi request reprint Escherichia coli sequence type ST131 as the major cause of serious multidrug-resistant E. coli infections in the United States
    James R Johnson
    Infectious Diseases, VA Medical Center, Minneapolis, MN 55417, USA
    Clin Infect Dis 51:286-94. 2010
    ..Escherichia coli sequence type ST131 (O25:H4), associated with the CTX-M-15 extended-spectrum beta-lactamase, has emerged internationally as a multidrug-resistant pathogen but has received little attention in the United States...
  34. ncbi request reprint Phylogenetic relationships among clonal groups of extraintestinal pathogenic Escherichia coli as assessed by multi-locus sequence analysis
    James R Johnson
    Mucosal and Vaccine Research Center, Minneapolis VA Medical Center, Infectious Diseases 111F, Minneapolis, MN 55417, USA
    Microbes Infect 8:1702-13. 2006
    ....
  35. ncbi request reprint Antimicrobial-resistant and extraintestinal pathogenic Escherichia coli in retail foods
    James R Johnson
    Mucosal and Vaccine Research Center, Veterans Affairs Medical Center, and Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55417, USA
    J Infect Dis 191:1040-9. 2005
    ..coli (ExPEC) strains and, increasingly, with antimicrobial resistance. The food supply may disseminate ExPEC and antimicrobial-resistant E. coli...
  36. ncbi request reprint Host-pathogen relationships among Escherichia coli isolates recovered from men with febrile urinary tract infection
    James R Johnson
    Mucosal and Vaccine Research Center, Minneapolis Veterans Affairs Medical Center, Minneapolis, MN 55417, USA
    Clin Infect Dis 40:813-22. 2005
    ..Host-pathogen relationships in men with febrile urinary tract infection (FUTI) are poorly understood...
  37. pmc Distribution and characteristics of Escherichia coli clonal group A
    James R Johnson
    Infectious Diseases 111F, Minneapolis VA Medical Center, 1 Veterans Drive, Minneapolis, MN 55417, USA
    Emerg Infect Dis 11:141-5. 2005
    ..3%; p < 0.001) and those resistant to trimethoprim-sulfamethoxazole (10% vs. 1.7%; p < 0.001). The extensive antimicrobial resistance and virulence profiles of clonal group A may underlie its recent widespread emergence...
  38. ncbi request reprint Microbial virulence determinants and the pathogenesis of urinary tract infection
    James R Johnson
    Infectious Diseases 111F, Veterans Affairs Medical Center, One Veteran s Drive, Minneapolis, MN 55417, USA
    Infect Dis Clin North Am 17:261-78, viii. 2003
    ..Although the true evolutionary basis for ExPEC is unknown, the virulence factors of ExPEC serve as useful epidemiologic markers and in the future may provide effective targets for anti-UTI interventions...
  39. pmc Extraintestinal pathogenic Escherichia coli as a cause of invasive nonurinary infections
    James R Johnson
    Mucosal and Vaccine Research Center, VA Medical Center, University of Minnesota, Minneapolis, Minnesota 55417, USA
    J Clin Microbiol 41:5798-802. 2003
    ..e., extraintestinal pathogenic E. coli...
  40. pmc Enterococcal aggregation substance and binding substance are not major contributors to urinary tract colonization by Enterococcus faecalis in a mouse model of ascending unobstructed urinary tract infection
    James R Johnson
    Mucosal and Vaccine Research Center, VA Medical Center and Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55417, USA
    Infect Immun 72:2445-8. 2004
    ..These data suggest that AS and EBS do not contribute to upper or lower urinary tract colonization by E. faecalis and that growth in urine does not induce AS expression by strains carrying plasmids in the pCF10 family...
  41. pmc Identification of urovirulence traits in Escherichia coli by comparison of urinary and rectal E. coli isolates from dogs with urinary tract infection
    James R Johnson
    Medical Service, VA Medical Center and Department of Medicine, University of Minnesota, Minneapolis, 55417, USA
    J Clin Microbiol 41:337-45. 2003
    ....
  42. pmc Detection of the Escherichia coli group 2 polysaccharide capsule synthesis Gene kpsM by a rapid and specific PCR-based assay
    James R Johnson
    Mucosal and Vaccine Research Center, Minneapolis VA Medical Center, Minnesota
    J Clin Microbiol 42:1773-6. 2004
    ....
  43. ncbi request reprint Phylogenetic background and virulence profiles of fluoroquinolone-resistant clinical Escherichia coli isolates from the Netherlands
    James R Johnson
    Medical Service, Veterans Affairs Medical Center, Minneapolis, Minnesota, USA
    J Infect Dis 186:1852-6. 2002
    ..These FQREC resemble animal-source E. coli and presumably pose little threat to noncompromised hosts. Similar analysis of other FQREC is needed...
  44. doi request reprint Virulence genotypes and phylogenetic background of fluoroquinolone-resistant and susceptible Escherichia coli urine isolates from dogs with urinary tract infection
    James R Johnson
    Veterans Affairs Medical Center, Minneapolis, MN, USA
    Vet Microbiol 136:108-14. 2009
    ..They also identify dogs as a possible reservoir of drug-resistant ExPEC for transmission to other pets and humans...
  45. pmc Rapid and specific detection of Escherichia coli clonal group A by gene-specific PCR
    James R Johnson
    Infectious Diseases 111F, Minneapolis VA Medical Center, One Veterans Dr, Minneapolis, MN 55417, USA
    J Clin Microbiol 42:2618-22. 2004
    ..The new CGA-specific PCR assay, which exhibited interlaboratory reproducibility and stability under various experimental conditions, should allow the rapid and specific detection of CGA by any laboratory equipped for diagnostic PCR...
  46. pmc Rapid and specific detection of the O15:K52:H1 clonal group of Escherichia coli by gene-specific PCR
    James R Johnson
    Mucosal and Vaccine Research Center, Minneapolis VA Medical Center, 1 Veterans Dr, Minneapolis, MN 55417, USA
    J Clin Microbiol 42:3841-3. 2004
    ..e., G594A. In experiments comparing various reference typing methods, the new primers provided 100% sensitivity and specificity for the O15:K52:H1 clonal group, including 162 diverse clinical and reference E. coli isolates...
  47. ncbi request reprint Uropathogenic Escherichia coli as agents of diverse non-urinary tract extraintestinal infections
    James R Johnson
    Medical Service, Veterans Affairs Medical Center, and Department of Medicine, University of Minnesota, Minneapolis, MN 55417, USA
    J Infect Dis 186:859-64. 2002
    ..They also provide novel evidence that multidrug-resistant epidemic clonal group A can cause extraintestinal infections other than uncomplicated urinary tract infections and can cause them in hosts other than young women...
  48. ncbi request reprint Extraintestinal pathogenic Escherichia coli: "the other bad E coli"
    James R Johnson
    Infectious Diseases 111F, Medical Service, Veterans Affairs Medical Center, 1 Veterans Drive, Minneapolis, MN 55417, USA
    J Lab Clin Med 139:155-62. 2002
    ....
  49. ncbi request reprint Phylogenetic origin and virulence genotype in relation to resistance to fluoroquinolones and/or extended-spectrum cephalosporins and cephamycins among Escherichia coli isolates from animals and humans
    James R Johnson
    Medical Service, Veterans Affairs Medical Center, Minneapolis, MN 55417, USA
    J Infect Dis 188:759-68. 2003
    ..Thus, in E. coli, antibiotic resistance predicts phylogenetic background and virulence potential in a complex, context-dependent fashion...
  50. ncbi request reprint Extended virulence genotypes and phylogenetic background of Escherichia coli isolates from patients with cystitis, pyelonephritis, or prostatitis
    James R Johnson
    Medical Service, VA Medical Center, Minneapolis, MN 55417, USA
    J Infect Dis 191:46-50. 2005
    ..Thus, interventions that target conserved uro-VFs may be possible, despite the likely existence of syndrome-specific pathogenetic mechanisms and/or host defense systems...
  51. pmc Virulence genotype and phylogenetic origin in relation to antibiotic resistance profile among Escherichia coli urine sample isolates from Israeli women with acute uncomplicated cystitis
    James R Johnson
    Infectious Diseases, Minneapolis VA Medical Center, One Veterans Dr, Minneapolis, MN 55417, USA
    Antimicrob Agents Chemother 49:26-31. 2005
    ..The basis for the concentration of ciprofloxacin resistance in non-B2 phylogenetic groups remains unknown...
  52. pmc High-frequency secondary mutations after suicide-driven allelic exchange mutagenesis in extraintestinal pathogenic Escherichia coli
    James R Johnson
    Mucosal and Vaccine Research Center, Infectious Diseases 111F, Veterans Affairs Medical Center, One Veterans Drive, Minneapolis, MN 55417, USA
    J Bacteriol 185:5301-5. 2003
    ..Caution is warranted when attributing the observed phenotypic changes to the intended mutation...
  53. pmc Isolation and molecular characterization of nalidixic acid-resistant extraintestinal pathogenic Escherichia coli from retail chicken products
    James R Johnson
    Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55417, USA
    Antimicrob Agents Chemother 47:2161-8. 2003
    ....
  54. pmc Global molecular epidemiology of the O15:K52:H1 extraintestinal pathogenic Escherichia coli clonal group: evidence of distribution beyond Europe
    James R Johnson
    Medical Service, Minneapolis VA Medical Center, MN 55417, USA
    J Clin Microbiol 40:1913-23. 2002
    ..These findings indicate that the O15:K52:H1 clonal group is broadly distributed beyond Europe, exhibits previously unrecognized phenotypic and genotypic diversity, and contributes significantly to extraintestinal infections in humans...
  55. ncbi request reprint Virulence factor profiles and phylogenetic background of Escherichia coli isolates from veterans with bacteremia and uninfected control subjects
    Mark R Sannes
    Mucosal and Vaccine Research Center and Medical Service, Veterans Affairs Medical Center, Minneapolis, Minnesota, USA
    J Infect Dis 190:2121-8. 2004
    ..The present study sought to determine specifically which virulence factors (VFs) and phylogenetic groups of E. coli are epidemiologically associated with bacteremia...
  56. pmc Escherichia coli sequence type 131 (ST131) subclone H30 as an emergent multidrug-resistant pathogen among US veterans
    Aylin Colpan
    University of Minnesota, Minneapolis
    Clin Infect Dis 57:1256-65. 2013
    ..Escherichia coli sequence type 131 (ST131), typically fluoroquinolone-resistant (FQ-R) and/or extended-spectrum β-lactamase (ESBL)-producing, has emerged globally. We assessed its prevalence and characteristics among US veterans...
  57. ncbi request reprint Antimicrobial resistance of Escherichia coli strains isolated from urine of women with cystitis or pyelonephritis and feces of dogs and healthy humans
    Mark R Sannes
    Medical Service, Minneapolis Veterans Affairs Medical Center, Minneapolis, MN 55417, USA
    J Am Vet Med Assoc 225:368-73. 2004
    ..To assess the prevalence and patterns of antimicrobial resistance among Escherichia coli strains isolated from the urine of women with cystitis or pyelonephritis and from fecal samples from dogs and healthy humans...
  58. doi request reprint Predictors of antimicrobial-resistant Escherichia coli in the feces of vegetarians and newly hospitalized adults in Minnesota and Wisconsin
    Mark R Sannes
    Infectious Diseases Section, Veterans Affairs Medical Center, Minneapolis, MN 55417, USA
    J Infect Dis 197:430-4. 2008
    ..coli did not prove to be such. Other exposures, including household-, community-, and population-level effects, may be more important...
  59. ncbi request reprint OxyR contributes to the virulence of a Clonal Group A Escherichia coli strain (O17:K+:H18) in animal models of urinary tract infection, subcutaneous infection, and systemic sepsis
    James R Johnson
    Veterans Affairs Medical Center, 1 Veterans Drive, Minneapolis, MN 55417, USA University of Minnesota, 420 Delaware St S E, Minneapolis, MN 55455, USA
    Microb Pathog 64:1-5. 2013
    ..coli infections. ..
  60. ncbi request reprint Epidemiological correlates of virulence genotype and phylogenetic background among Escherichia coli blood isolates from adults with diverse-source bacteremia
    James R Johnson
    Medical Service, Infectious Diseases, Minneapolis Veterans Affairs Medical Center, Minneapolis, MN 55417, USA
    J Infect Dis 185:1439-47. 2002
    ..coli...
  61. ncbi request reprint Geographical distribution of antimicrobial resistance among Escherichia coli causing acute uncomplicated pyelonephritis in the United States
    Mark R Sannes
    Medical Service, Infectious Disease Section 111F, VA Medical Center, University of Minnesota, One Veterans Drive, Minneapolis, MN 55417, USA
    FEMS Immunol Med Microbiol 42:213-8. 2004
    ..coli within the US, which is relevant both to clinical practice and to understanding the basis for the current epidemic of antimicrobial resistance in E. coli...
  62. pmc Colonization with extraintestinal pathogenic Escherichia coli among nursing home residents and its relationship to fluoroquinolone resistance
    Joel N Maslow
    Section of Infectious Diseases, Veterans Affairs Medical Center, University and Woodland Ave, Philadelphia, PA 19104, USA
    Antimicrob Agents Chemother 48:3618-20. 2004
    ..coli, and 51% were colonized with fluoroquinolone-resistant E. coli. Among 80 unique isolates, adhesins correlated negatively and aerobactin correlated positively with fluoroquinolone resistance...
  63. ncbi request reprint A disseminated multidrug-resistant clonal group of uropathogenic Escherichia coli in pyelonephritis
    James R Johnson
    Medical Service, Minneapolis VA Medical Center and Department of Medicine, University of Minnesota, Minneapolis, MN, USA
    Lancet 359:2249-51. 2002
    ..Thus, CGA is broadly disseminated and contributes to co-trimoxazole resistance in pyelonephritis as well as in cystitis...
  64. pmc Susceptibility to alternative oral antimicrobial agents in relation to sequence type ST131 status and Coresistance phenotype among recent Escherichia coli isolates from U.S. veterans
    James R Johnson
    Veterans Affairs Medical Center, Minneapolis, Minnesota, USA
    Antimicrob Agents Chemother 57:4856-60. 2013
    ..coli isolates resistant to fluoroquinolones, ESCs, and/or TMP-SMZ and suggest that determination of ST131 status could help guide initial antimicrobial selection, pending susceptibility results. ..
  65. ncbi request reprint Complications of Foley catheters--is infection the greatest risk?
    Anne Marie Leuck
    VA Medical Center, University of Minnesota, Minneapolis, Minnesota, USA
    J Urol 187:1662-6. 2012
    ..Although symptomatic urinary tract infection and asymptomatic bacteriuria are frequently discussed, genitourinary trauma receives comparatively little attention...
  66. ncbi request reprint Urinary tract infection in male veterans: treatment patterns and outcomes
    Dimitri M Drekonja
    Minneapolis Veterans Affairs Health Care System, Minneapolis, MN 55417, USA
    JAMA Intern Med 173:62-8. 2013
    ..The optimal treatment duration and current treatment patterns for urinary tract infection (UTI) in men are unknown. We used Veterans Affairs administrative data to study male UTI treatment and outcomes...
  67. ncbi request reprint Concurrent fecal colonization with extraintestinal pathogenic Escherichia coli in a homosexual man with recurrent urinary tract infection and in his male sex partner
    James R Johnson
    Medical Service, Veterans Affairs Medical Center, and Department of Medicine, University of Minnesota, Minneapolis, MN, 55417, USA
    Clin Infect Dis 35:E65-8. 2002
    ..coli O1/O2:K1:H7 clonal group (which is associated with urosepsis), whereas genetically distinct E. coli strains were recovered from a jar of petroleum jelly used by the couple during sexual activity...
  68. pmc Systematic review of the literature on comparative effectiveness of antiviral treatments for chronic hepatitis B infection
    Tatyana A Shamliyan
    Division of Health Policy and Management, Minnesota Evidence based Practice Center, University of Minnesota School of Public Health, 420 Delaware Street SE, D330 5 Mayo MMC 729, Minneapolis, MN 55455, USA
    J Gen Intern Med 26:326-39. 2011
    ..To evaluate the comparative effectiveness of antiviral drugs in adults with chronic hepatitis B monoinfection for evidence-based decision-making...
  69. ncbi request reprint Antimicrobial urinary catheters: a systematic review
    Dimitri M Drekonja
    Infectious Diseases, Minneapolis Veterans Affairs Medical Center, Minneapolis, MN, USA
    Expert Rev Med Devices 5:495-506. 2008
    ....
  70. doi request reprint Treatment of non-life-threatening methicillin-resistant Staphylococcus aureus infections with alternative antimicrobial agents: a 2-year retrospective review
    Dimitri M Drekonja
    Minneapolis Veterans Affairs Medical Center, Minneapolis, MN 55417, USA
    Diagn Microbiol Infect Dis 63:201-7. 2009
    ..Alternative agents can be used successfully to treat non-life-threatening MRSA infections in appropriate patients. Randomized comparative trials are needed...
  71. doi request reprint Foley catheter practices and knowledge among Minnesota physicians
    Dimitri M Drekonja
    Minneapolis Veterans Affairs Medical Center, 1 Veterans Drive, Minneapolis, MN 55417, USA
    Am J Infect Control 38:694-700. 2010
    ....
  72. ncbi request reprint Medical and economic impact of extraintestinal infections due to Escherichia coli: focus on an increasingly important endemic problem
    Thomas A Russo
    Division of Infectious Diseases, Department of Medicine and Microbiology, The Center for Microbial Pathogenesis, VA Medical Center, University at Buffalo, Buffalo, NY 14214 3001, USA
    Microbes Infect 5:449-56. 2003
    ..coli. New treatments and prevention measures will be needed for improved outcomes and a diminished disease burden...
  73. ncbi request reprint Antiviral therapy for adults with chronic hepatitis B: a systematic review for a National Institutes of Health Consensus Development Conference
    Tatyana A Shamliyan
    Minnesota Evidence based Practice Center, Division of Health Policy and Management, University of Minnesota School of Public Health, 420 Delaware Street SE, Minneapolis, MN 55455, USA
    Ann Intern Med 150:111-24. 2009
    ..Chronic hepatitis B infection can lead to liver failure, hepatocellular carcinoma, and death...
  74. doi request reprint Internet survey of Foley catheter practices and knowledge among Minnesota nurses
    Dimitri M Drekonja
    Minneapolis Veterans Affairs Medical Center, Minneapolis, MN 55417, USA
    Am J Infect Control 38:31-7. 2010
    ..Although nurses insert and care for many Foley catheters, little is known about nurses' attitudes and knowledge regarding indications for catheter use or methods to prevent catheter-associated urinary tract infection (CAUTI)...
  75. doi request reprint Urinary tract infections
    Dimitri M Drekonja
    Minneapolis Veterans Affairs Medical Center, Infectious Diseases 111F, 1 Veterans Drive, Minneapolis, MN 55417, USA
    Prim Care 35:345-67, vii. 2008
    ..Further research is needed regarding rapid diagnosis of UTI, accurate presumptive identification of patients with resistant pathogens, and development of new antimicrobials for drug-resistant UTI...
  76. pmc Community transmission in the United States of a CTX-M-15-producing sequence type ST131 Escherichia coli strain resulting in death
    Robert C Owens
    Division of Infectious Diseases, Department of Medicine, Maine Medical Center, Portland, Maine, USA
    J Clin Microbiol 49:3406-8. 2011
    ..Susceptibility results (reported on day 4) showed resistance to the initially selected regimen...
  77. ncbi request reprint Outcomes of a Hepatitis C screening program at a large urban VA medical center
    Holly Groom
    Department of Medicine, University of Minnesota, Minneapolis, MN, USA
    J Clin Gastroenterol 42:97-106. 2008
    ..To determine the outcomes of implementing clinical care guidelines for Hepatitis C screening, evaluation, and treatment in a large urban Veterans Affairs Medical Center...
  78. ncbi request reprint Phylogenetic distribution of virulence-associated genes among Escherichia coli isolates associated with neonatal bacterial meningitis in the Netherlands
    James R Johnson
    Medical Service, Veterans Affairs Medical Center, and Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55417, USA
    J Infect Dis 185:774-84. 2002
    ..These findings provide novel insights into the phylogenetic origins of NBM-associated E. coli and suggest numerous new potential targets for preventive interventions against this dire disease...
  79. pmc Virulence factors in Escherichia coli
    James R Johnson
    J Clin Microbiol 43:6221; author reply 6221-2. 2005
  80. pmc Drug-resistant Escherichia coli, Rural Idaho
    Elizabeth L Hannah
    University of Utah School of Medicine, Salt Lake City, Utah 84132 2901, USA
    Emerg Infect Dis 11:1614-7. 2005
    ..coli was associated with recent use of antimicrobial agents in the household. Household clustering of drug-resistant E. coli was observed. Most carriers of drug-resistant E. coli lacked conventional risk factors...
  81. pmc Fluoroquinolone-resistant Escherichia coli, Indonesia
    Kuntaman Kuntaman
    Airlangga University, Surabaya, Indonesia
    Emerg Infect Dis 11:1363-9. 2005
    ..Hypervirulent strains from the B2 cluster were underrepresented (1%). Because FQ-resistant E. coli can cause disease, especially nosocomial infections in immunocompromised patients, spread of such strains must be stopped...
  82. pmc Quinolone-resistant uropathogenic Escherichia coli strains from phylogenetic group B2 have fewer virulence factors than their susceptible counterparts
    Juan P Horcajada
    Service of Infectious Diseases, Hospital Clinic Universitari IDIBAPS, Villaroel 170, 08036 Barcelona, Spain
    J Clin Microbiol 43:2962-4. 2005
    ..Hemolysin, cytotoxic necrotizing factor 1, and S and F1C fimbriae genes were less prevalent among nalidixic acid-resistant E. coli strains. Quinolone resistance may be associated with a decrease in the presence of some virulence factors...
  83. ncbi request reprint Possible animal origin of human-associated, multidrug-resistant, uropathogenic Escherichia coli
    Meena Ramchandani
    Division of Infectious Diseases, School of Public Health, University of California, Berkeley, CA 94720, USA
    Clin Infect Dis 40:251-7. 2005
    ..The present study attempted to determine if CgA strains could be traced to food animals...
  84. ncbi request reprint Haemolytic Escherichia coli isolated from dogs with diarrhea have characteristics of both uropathogenic and necrotoxigenic strains
    Marjanca Starcic
    Department of Infectious Diseases and Immunology, Division of Bacteriology, Veterinary Faculty, University of Utrecht, P O Box 80 165, 3584 CL Utrecht, The Netherlands
    Vet Microbiol 85:361-77. 2002
    ..If this is the case and this type of isolate is "bifunctional", domestic animals likely constitute a reservoir of NTEC strains which can be also pathogenic for humans...
  85. ncbi request reprint Virulence factors predict Escherichia coli colonization patterns among human and animal household members
    Andrew C Murray
    Ann Intern Med 140:848-9. 2004
  86. pmc Cephalosporin-resistant Escherichia coli among summer camp attendees with salmonellosis
    Guillem Prats
    Department of Microbiology, Universitat Autonoma, Hospital Vall d Hebron, P Vall d Hebron 119 129, 08035 Barcelona, Spain
    Emerg Infect Dis 9:1273-80. 2003
  87. pmc Escherichia coli pathogenicity island-like domains
    James R Johnson
    Infect Immun 70:7185; author reply 7185-6. 2002
  88. pmc IroN functions as a siderophore receptor and is a urovirulence factor in an extraintestinal pathogenic isolate of Escherichia coli
    Thomas A Russo
    Department of Medicine, University of Buffalo, New York 14214, USA
    Infect Immun 70:7156-60. 2002
    ..Taken together, these findings establish that IroN is a siderophore receptor and a urovirulence factor. However, uncertainty exists as to the mechanism(s) via which IroN contributes to urovirulence...
  89. pmc Characterization of Stg fimbriae from an avian pathogenic Escherichia coli O78:K80 strain and assessment of their contribution to colonization of the chicken respiratory tract
    Maria H Lymberopoulos
    INRS Institut Armand Frappier, 531 Boul des Prairies, Laval, Quebec, Canada
    J Bacteriol 188:6449-59. 2006
    ..Stg fimbriae, together with other adhesins, may therefore contribute to the colonization of avian respiratory tissues by certain APEC strains...
  90. ncbi request reprint Quinolone, fluoroquinolone and trimethoprim/sulfamethoxazole resistance in relation to virulence determinants and phylogenetic background among uropathogenic Escherichia coli
    Eva Moreno
    Microbiology Department, Hospital Vall d Hebron, Universitat Autonoma de Barcelona, Barcelona, Spain
    J Antimicrob Chemother 57:204-11. 2006
    ..The goal of this study was to assess how resistance to quinolones, fluoroquinolones and trimethoprim/sulfamethoxazole relates to the virulence potential and phylogenetic background of clinical Escherichia coli isolates...
  91. pmc Risk factors for trimethoprim-sulfamethoxazole resistance in patients with acute uncomplicated cystitis
    Richard Colgan
    Department of Family Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Antimicrob Agents Chemother 52:846-51. 2008
    ..TMP-SMX r isolates were more likely to belong to CGA. Knowledge of these risk factors for TMP-SMX resistance could facilitate the accurate selection of empirical therapy...
  92. pmc Modulation of host innate immune response in the bladder by uropathogenic Escherichia coli
    Benjamin K Billips
    Department of Urology, Northwestern University Feinberg School of Medicine, 16 703 Tarry, 303 East Chicago Avenue, Chicago, IL 60611, USA
    Infect Immun 75:5353-60. 2007
    ..These data indicate that a broad phylogenetic range of clinical E. coli isolates, including UPEC, may evade the activation of innate immune response in the urinary tract, thereby providing a pathogenic advantage...
  93. ncbi request reprint Fim operon variation in the emergence of Enterohemorrhagic Escherichia coli: an evolutionary and functional analysis
    Nurmohammad Shaikh
    Washington University School of Medicine, St Louis, MO 63110, USA
    FEMS Microbiol Lett 273:58-63. 2007
    ..coli (EHEC) tested. An N135K FimH mutation in the two most evolved O157:H7 clusters is not found in other EHEC. These data refine the evolutionary history of an emerging pathogen...
  94. pmc Enteroaggregative Escherichia coli related to uropathogenic clonal group A
    Faith Wallace-Gadsden
    Department of Biology, Haverford College, Haverford, Pennsylvania 19041, USA
    Emerg Infect Dis 13:757-60. 2007
    ..coli clonal group A belongs. This finding suggests a recent common ancestor for these distinct groups of pathogenic E. coli...
  95. pmc Occurrence of antibiotic-resistant uropathogenic Escherichia coli clonal group A in wastewater effluents
    Laura A Boczek
    US Environmental Protection Agency, Cincinnati, OH, USA
    Appl Environ Microbiol 73:4180-4. 2007
    ..The occurrence of "human-type" CGA in wastewater effluents demonstrates a potential mode for the dissemination of this clonal group in the environment, with possible secondary transmission to new human or animal hosts...
  96. pmc A killed, genetically engineered derivative of a wild-type extraintestinal pathogenic E. coli strain is a vaccine candidate
    Thomas A Russo
    Department of Medicine, University at Buffalo, and Veterans Administration Western New York Healthcare System, Buffalo, NY 14214, United States
    Vaccine 25:3859-70. 2007
    ..Taken together, these studies support the concept that formalin-killed genetically engineered ExPEC derivatives are whole cell vaccine candidates to prevent infections due to ExPEC...
  97. pmc The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes
    Timothy J Johnson
    Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, 1802 Elwood Drive, VMRI 2, Iowa State University, Ames, IA 50011, USA
    J Bacteriol 189:3228-36. 2007
    ..Future studies are necessary to assess the ability of APEC to overcome the hurdles necessary for such a food-borne transmission, and epidemiological studies are required to confirm that such a phenomenon actually occurs...
  98. ncbi request reprint Determination of Escherichia coli O types by allele-specific polymerase chain reaction: application to the O types involved in human septicemia
    Olivier Clermont
    Institut National de la Santé et de la Recherche Médicale INSERM U722 and Faculté de Médecine Xavier Bichat, Universite Paris 7 Denis Diderot, 75018 Paris, France
    Diagn Microbiol Infect Dis 57:129-36. 2007
    ..coli. This method allowed us to determine the O types of 153 strains previously typed using reference methods with an accuracy exceeding 90%. Moreover, some rough or nonagglutinating strains can be successfully typed...
  99. pmc Ciprofloxacin-resistant gram-negative bacilli in the fecal microflora of children
    Xuan Qin
    Department of Laboratory Medicine, Children s Hospital and Regional Medical Center, Seattle, WA, USA
    Antimicrob Agents Chemother 50:3325-9. 2006
    ..The sources of these resistant organisms are unknown. This underrecognized reservoir of drug-resistant potential pathogens poses public health challenges...
  100. pmc Iha from an Escherichia coli urinary tract infection outbreak clonal group A strain is expressed in vivo in the mouse urinary tract and functions as a catecholate siderophore receptor
    Simon Leveille
    INRS Institut Armand Frappier, 531 Boul des Prairies, Laval H7V 1B7, Quebec, Canada
    Infect Immun 74:3427-36. 2006
    ..Iha thus represents a Fur-regulated catecholate siderophore receptor that, uniquely, exhibits an adherence-enhancing phenotype and is the first described urovirulence factor identified in a CGA strain...
  101. pmc Clonal analysis reveals high rate of structural mutations in fimbrial adhesins of extraintestinal pathogenic Escherichia coli
    Scott J Weissman
    Division of Infectious Disease, Immunology and Rheumatology, Department of Pediatrics, University of Washington, Seattle, WA, USA
    Mol Microbiol 59:975-88. 2006
    ..coli are under strong positive selection (Dn/Ds > 1 for fimH and papG) for functionally adaptive amino acid replacements...

Research Grants3

  1. Resistant E. coli in Humans and Poultry
    James Johnson; Fiscal Year: 2004
    ..Does the research address a current and compelling problem of antimicrobial resistance that is of high public health importance and for which research is needed? [yes]. ..
  2. Resistant E.coli in Humans and Poultry
    James Johnson; Fiscal Year: 2005
    ..Does the research address a current and compelling problem of antimicrobial resistance that is of high public health importance and for which research is needed? [yes]. ..
  3. Infectious Disease Training in Clinical Investigation
    James Johnson; Fiscal Year: 2007
    ..unreadable] [unreadable]..