Gary Johnson

Summary

Affiliation: University of Colorado Health Sciences Center
Country: USA

Publications

  1. ncbi request reprint Signal transduction. Scaffolding proteins--more than meets the eye
    Gary Johnson
    Department of Pharmacology, University of Colorado Health Sciences Center, Denver, CO 80262, USA
    Science 295:1249-50. 2002
  2. ncbi request reprint Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases
    Gary L Johnson
    Department of Pharmacology, University of Colorado Health Sciences Center, Denver, CO 80262, USA
    Science 298:1911-2. 2002
  3. ncbi request reprint Combined use of oligonucleotide and tissue microarrays identifies cancer/testis antigens as biomarkers in lung carcinoma
    Michio Sugita
    Department of Pathology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    Cancer Res 62:3971-9. 2002
  4. pmc Bradykinin antagonist dimer, CU201, inhibits the growth of human lung cancer cell lines by a "biased agonist" mechanism
    Daniel Chan
    Lung Cancer Program, University of Colorado Cancer Center, Denver, CO 80262, USA
    Proc Natl Acad Sci U S A 99:4608-13. 2002
  5. ncbi request reprint ZD1839, a selective epidermal growth factor receptor tyrosine kinase inhibitor, alone and in combination with radiation and chemotherapy as a new therapeutic strategy in non-small cell lung cancer
    David Raben
    Department of Radiation Oncology, University of Colorado Comprehensive Cancer Center, Denver, CO 80010 0510, USA
    Semin Oncol 29:37-46. 2002
  6. ncbi request reprint Ablation of MEK kinase 1 suppresses intimal hyperplasia by impairing smooth muscle cell migration and urokinase plasminogen activator expression in a mouse blood-flow cessation model
    Yan Li
    Department of Internal Medicine and Therapeutics, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
    Circulation 111:1672-8. 2005
  7. ncbi request reprint MEKK1 regulates the AP-1 dimer repertoire via control of JunB transcription and Fra-2 protein stability
    Bruce D Cuevas
    Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7365, USA
    Oncogene 24:801-9. 2005
  8. pmc JNK regulates the release of proapoptotic mitochondrial factors in reovirus-infected cells
    Penny Clarke
    Dept of Neurology B 182, University of Colorado Health Sciences Center, 4200 East 9th Ave, Denver, CO 80262, USA
    J Virol 78:13132-8. 2004
  9. ncbi request reprint MEKK2 regulates the coordinate activation of ERK5 and JNK in response to FGF-2 in fibroblasts
    Kamala Kesavan
    Department of Pharmacology and University of Colorado Cancer Center, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    J Cell Physiol 199:140-8. 2004
  10. ncbi request reprint Rac2D57N, a dominant inhibitory Rac2 mutant that inhibits p38 kinase signaling and prevents surface ruffling in bone-marrow-derived macrophages
    Amy N Abell
    Department of Pharmacology, University of Colorado Health Sciences Center, 4200 East Ninth Ave, Denver, CO 80262, USA
    J Cell Sci 117:243-55. 2004

Collaborators

Detail Information

Publications49

  1. ncbi request reprint Signal transduction. Scaffolding proteins--more than meets the eye
    Gary Johnson
    Department of Pharmacology, University of Colorado Health Sciences Center, Denver, CO 80262, USA
    Science 295:1249-50. 2002
  2. ncbi request reprint Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases
    Gary L Johnson
    Department of Pharmacology, University of Colorado Health Sciences Center, Denver, CO 80262, USA
    Science 298:1911-2. 2002
    ..The p38 MAPKs are activated by inflammatory cytokines and environmental stresses and may contribute to diseases like asthma and autoimmunity...
  3. ncbi request reprint Combined use of oligonucleotide and tissue microarrays identifies cancer/testis antigens as biomarkers in lung carcinoma
    Michio Sugita
    Department of Pathology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    Cancer Res 62:3971-9. 2002
    ....
  4. pmc Bradykinin antagonist dimer, CU201, inhibits the growth of human lung cancer cell lines by a "biased agonist" mechanism
    Daniel Chan
    Lung Cancer Program, University of Colorado Cancer Center, Denver, CO 80262, USA
    Proc Natl Acad Sci U S A 99:4608-13. 2002
    ..CU201 and similar compounds offer hope of becoming a new form of targeted therapy for tumors with neuroendocrine properties...
  5. ncbi request reprint ZD1839, a selective epidermal growth factor receptor tyrosine kinase inhibitor, alone and in combination with radiation and chemotherapy as a new therapeutic strategy in non-small cell lung cancer
    David Raben
    Department of Radiation Oncology, University of Colorado Comprehensive Cancer Center, Denver, CO 80010 0510, USA
    Semin Oncol 29:37-46. 2002
    ..Future studies will certainly combine ZD1839 with chemotherapy or radiation. ZD1839 also may be effective as a chemoprevention agent because premalignant lesions often overexpress epidermal growth factor receptor...
  6. ncbi request reprint Ablation of MEK kinase 1 suppresses intimal hyperplasia by impairing smooth muscle cell migration and urokinase plasminogen activator expression in a mouse blood-flow cessation model
    Yan Li
    Department of Internal Medicine and Therapeutics, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
    Circulation 111:1672-8. 2005
    ..Although MEK kinase 1 (MEKK1) has been shown to regulate cell migration and urokinase plasminogen activator (uPA) expression, the precise role of MEKK1 in this process remains unknown...
  7. ncbi request reprint MEKK1 regulates the AP-1 dimer repertoire via control of JunB transcription and Fra-2 protein stability
    Bruce D Cuevas
    Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7365, USA
    Oncogene 24:801-9. 2005
    ..Controlling the repertoire of a transcription factor complex is a newly defined function for an MAPK kinase kinase...
  8. pmc JNK regulates the release of proapoptotic mitochondrial factors in reovirus-infected cells
    Penny Clarke
    Dept of Neurology B 182, University of Colorado Health Sciences Center, 4200 East 9th Ave, Denver, CO 80262, USA
    J Virol 78:13132-8. 2004
    ..This is the first report demonstrating that JNK is associated with regulation of mitochondrial pathways of apoptosis following viral infection...
  9. ncbi request reprint MEKK2 regulates the coordinate activation of ERK5 and JNK in response to FGF-2 in fibroblasts
    Kamala Kesavan
    Department of Pharmacology and University of Colorado Cancer Center, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    J Cell Physiol 199:140-8. 2004
    ..Bacterial lipopolysaccharide (LPS) and TNFalpha neither activate ERK5 nor require MEKK2 for JNK activation, demonstrating specificity of MEKK2 in FGF-2 receptor signaling and control of cytokine gene expression...
  10. ncbi request reprint Rac2D57N, a dominant inhibitory Rac2 mutant that inhibits p38 kinase signaling and prevents surface ruffling in bone-marrow-derived macrophages
    Amy N Abell
    Department of Pharmacology, University of Colorado Health Sciences Center, 4200 East Ninth Ave, Denver, CO 80262, USA
    J Cell Sci 117:243-55. 2004
    ..Enhanced binding of Rac2D57N to its upstream regulators would inhibit Rac-dependent effects on actin cytoskeletal dynamics and p38 kinase signaling...
  11. ncbi request reprint Rac-MEKK3-MKK3 scaffolding for p38 MAPK activation during hyperosmotic shock
    Mark T Uhlik
    Department of Pharmacology and the Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine Chapel Hill, NC 27599 7365, USA
    Nat Cell Biol 5:1104-10. 2003
    ..The Rac-OSM-MEKK3-MKK3 complex is the mammalian counterpart of the CDC42-STE50-STE11-Pbs2 complex in Saccharomyces cerevisiae that is required for the regulation of p38 activity...
  12. ncbi request reprint MEF2C regulates c-Jun but not TNF-alpha gene expression in stimulated mast cells
    Xudong Wei
    Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80206, USA
    Eur J Immunol 33:2903-9. 2003
    ....
  13. ncbi request reprint Roles for TAB1 in regulating the IL-1-dependent phosphorylation of the TAB3 regulatory subunit and activity of the TAK1 complex
    Heidi Mendoza
    MRC Protein Phosphorylation Unit, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 409:711-22. 2008
    ....
  14. ncbi request reprint CCM1 and CCM2 protein interactions in cell signaling: implications for cerebral cavernous malformations pathogenesis
    Jon S Zawistowski
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Hum Mol Genet 14:2521-31. 2005
    ..These data indicate that the genetic heterogeneity observed in familial CCM may reflect mutation of different molecular members of a coordinated signaling complex...
  15. ncbi request reprint Hyperosmotic induction of mitogen-activated protein kinase scaffolding
    Thomas L Hilder
    Department of Pharmacology and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Methods Enzymol 428:297-312. 2007
    ....
  16. ncbi request reprint Integrated activation of MAP3Ks balances cell fate in response to stress
    Ann M Winter-Vann
    Department of Pharmacology, 1108 Mary Ellen Jones Bldg, Campus Box 7365, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7365, USA
    J Cell Biochem 102:848-58. 2007
    ..The interrelationships among different stressors are discussed, with an emphasis on how the balance of signaling through MAP3Ks controls the MAPK response to determine cell fate...
  17. ncbi request reprint MEKK4 stimulation of p38 and JNK activity is negatively regulated by GSK3beta
    Amy N Abell
    Department of Pharmacology and the Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7365, USA
    J Biol Chem 282:30476-84. 2007
    ..Thus, control of MEKK4 dimerization is regulated both positively and negatively by its interaction with specific proteins...
  18. pmc The c-jun kinase/stress-activated pathway: regulation, function and role in human disease
    Gary L Johnson
    University of North Carolina at Chapel Hill, Department of Pharmacology, and Lineberger Comprehensive Cancer Center, 31 331 LCC Chapel Hill, NC 27599, USA
    Biochim Biophys Acta 1773:1341-8. 2007
    ..In this review, we present our current understanding of JNK regulation and their involvement in homeostasis and dysregulation in human disease...
  19. pmc MEKK1 mediates the ubiquitination and degradation of c-Jun in response to osmotic stress
    Yan Xia
    Department of Neuro Oncology, Unit 1002, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030, USA
    Mol Cell Biol 27:510-7. 2007
    ..Furthermore, apoptosis induced by osmotic stress was suppressed by overexpression of c-Jun, indicating that the downregulation of c-Jun promotes apoptosis...
  20. pmc Analysis of orthologous gene expression between human pulmonary adenocarcinoma and a carcinogen-induced murine model
    Robert S Stearman
    Department of Medicine Pulmonary Sciences and Critical Care Medicine, University of Colorado Health Sciences Center, Denver, USA
    Am J Pathol 167:1763-75. 2005
    ..Thus, the A/J mouse-urethane model reflects significant molecular details of human lung AC, and comparison of changes in orthologous gene expression may provide novel insights into lung carcinogenesis...
  21. pmc Ablation of MEKK4 kinase activity causes neurulation and skeletal patterning defects in the mouse embryo
    Amy N Abell
    Department of Pharmacology, University of North Carolina, Chapel Hill, 27599 7365, USA
    Mol Cell Biol 25:8948-59. 2005
    ..Together, these data demonstrate MEKK4 regulation of p38 and that substrates downstream of p38 control cellular homeostasis. The findings are the first demonstration that MEKK4-regulated p38 activity is critical for neurulation...
  22. ncbi request reprint MEKK4 is an effector of the embryonic TRAF4 for JNK activation
    Amy N Abell
    Department of Pharmacology and Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7365, USA
    J Biol Chem 280:35793-6. 2005
    ..The findings identify MEKK4 as the MAPK kinase kinase for TRAF4 regulation of the JNK pathway...
  23. ncbi request reprint PB1 domains of MEKK2 and MEKK3 interact with the MEK5 PB1 domain for activation of the ERK5 pathway
    Kazuhiro Nakamura
    Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7365, USA
    J Biol Chem 278:36989-92. 2003
    ..The free PB1 domain of MEKK2 or MEKK3 functions effectively to inhibit the ERK5 pathway but not the p38 or JNK pathways, demonstrating the specific and unique requirement of the MEKK2 and MEKK3 PB1 domain in regulating ERK5 activation...
  24. pmc The MEKK1-JNK pathway plays a protective role in pressure overload but does not mediate cardiac hypertrophy
    Junichi Sadoshima
    Cardiovascular Research Institute, Department of Cell Biology and Molecular Medicine, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103, USA
    J Clin Invest 110:271-9. 2002
    ..MEKK1 also prevents apoptosis and inflammation, thereby protecting against heart failure and sudden death following cardiac pressure overload...
  25. pmc MEKK1 is essential for cardiac hypertrophy and dysfunction induced by Gq
    Tetsuo Minamino
    Center for Cardiovascular Development and The DeBakey Heart Center, Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 99:3866-71. 2002
    ..Thus, MEKK1 mediates cardiac hypertrophy induced by Galphaq in vivo and is a logical target for drug development in heart disease involving this pathway...
  26. pmc Reovirus-induced alterations in gene expression related to cell cycle regulation
    George J Poggioli
    Department of Microbiology, University of Colorado Health Sciences Center, Denver, Colorado 80220, USA
    J Virol 76:2585-94. 2002
    ..A hypothetical model describing serotype 3 reovirus-induced inhibition of cdc2 kinase is presented, and reovirus-induced perturbations of the G(1)-to-S, G(2)-to-M, and mitotic spindle checkpoints are discussed...
  27. ncbi request reprint Mixed-lineage kinase control of JNK and p38 MAPK pathways
    Kathleen A Gallo
    Department of Physiology, Michigan State University, East Lansing, Michigan 48824, USA
    Nat Rev Mol Cell Biol 3:663-72. 2002
    ..The Drosophila MLK Slipper regulates JNK to control dorsal closure during embryonic morphogenesis. In mammalian cells, MLKs are implicated in the control of apoptosis and are potential drug targets for many neurodegenerative diseases...
  28. ncbi request reprint TRAIL and inhibitors of apoptosis are opposing determinants for NF-kappaB-dependent, genotoxin-induced apoptosis of cancer cells
    Aaron C Spalding
    Department of Pharmacology, University of Colorado Cancer Center, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, Colorado, CO 80262, USA
    Oncogene 21:260-71. 2002
    ..These findings demonstrate that TRAIL signaling via its death receptors is a significant contributor to genotoxin-induced apoptosis in human epithelial carcinomas...
  29. ncbi request reprint MEKK1-induced apoptosis requires TRAIL death receptor activation and is inhibited by AKT/PKB through inhibition of MEKK1 cleavage
    Andrea H Bild
    Department of Pharmacology, University of Colorado, 2400 East Ninth Street, Denver, Colorado, CO 80262, USA
    Oncogene 21:6649-56. 2002
    ..Thus, MEKK1-induced apoptosis requires TRAIL death receptor activation and is blocked by AKT through inhibition of MEKK1 cleavage...
  30. ncbi request reprint Apoptosis stimulated by the 91-kDa caspase cleavage MEKK1 fragment requires translocation to soluble cellular compartments
    Thomas K Schlesinger
    Institute of Cellular Biology and Morphology, University of Lausanne, 1005 Lausanne, Switzerland
    J Biol Chem 277:10283-91. 2002
    ....
  31. ncbi request reprint Ubiquitylation of MEKK1 inhibits its phosphorylation of MKK1 and MKK4 and activation of the ERK1/2 and JNK pathways
    James A Witowsky
    Department of Pharmacology, University of Colorado Health Sciences Center and University of Colorado Cancer Center, Denver, Colorado 80262, USA
    J Biol Chem 278:1403-6. 2003
    ..MEKK1 ubiquitylation represents a mechanism for inhibiting the ability of a protein kinase to phosphorylate substrates and regulate downstream signaling pathways...
  32. ncbi request reprint MEKK1 is required for inducible urokinase-type plasminogen activator expression
    James Witowsky
    Department of Pharmacology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    J Biol Chem 278:5941-6. 2003
    ..Importantly, disrupted expression of MEKK2, a related MAPK kinase kinase, had no effect on uPA activity. Therefore, we conclude that MEKK1 expression is required for PMA- or FGF-2-induced signals to control uPA expression and function...
  33. pmc MEK kinase 2 and the adaptor protein Lad regulate extracellular signal-regulated kinase 5 activation by epidermal growth factor via Src
    Weiyong Sun
    Department of Pharmacology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    Mol Cell Biol 23:2298-308. 2003
    ....
  34. ncbi request reprint Role of the amino-terminal domains of MEKKs in the activation of NF kappa B and MAPK pathways and in the regulation of cell proliferation and apoptosis
    Christelle Bonvin
    Institute of Cellular Biology and Morphology, Lausanne University, Lausanne, Switzerland
    Cell Signal 14:123-31. 2002
    ..Our data show that the N-terminal domain of the MEKKs determines the specificity and the strength of activation of various intracellular signaling pathways and cellular responses...
  35. pmc MEKK1 regulates calpain-dependent proteolysis of focal adhesion proteins for rear-end detachment of migrating fibroblasts
    Bruce D Cuevas
    Department of Pharmacology, Craniofacial Biology, University of Colorado Health Sciences Center, Denver, CO 80262, USA
    EMBO J 22:3346-55. 2003
    ..Inhibition of ERK1/2 or calpain, but not of JNK, mimics MEKK1 deficiency. Therefore, MEKK1 regulates calpain-mediated substratum release of migrating fibroblasts...
  36. ncbi request reprint Inhibition of Src family kinases blocks epidermal growth factor (EGF)-induced activation of Akt, phosphorylation of c-Cbl, and ubiquitination of the EGF receptor
    C Kenneth Kassenbrock
    Department of Pathology, University of Colorado Health Sciences Center, Denver 80262, USA
    J Biol Chem 277:24967-75. 2002
    ..PP1 treatment blocks EGF-induced activation of the anti-apoptotic protein kinase Akt suggesting that Src may regulate activation of Akt, perhaps by a Src --> c-Cbl --> phosphatidylinositol 3'-kinase --> Akt pathway...
  37. ncbi request reprint Activation of chymotrypsin-like serine protease(s) during apoptosis detected by affinity-labeling of the enzymatic center with fluoresceinated inhibitor
    Jerzy Grabarek
    Brander Cancer Research Institute, New York Medical College, Valhalla, NY 10595, USA
    Int J Oncol 20:225-33. 2002
    ..Ser-protease(s) was different. Our approach makes it possible to simultaneously monitor activation of caspases and Ser proteases in the same live cells that are induced to apoptosis...
  38. pmc Noncanonical function of MEKK2 and MEK5 PB1 domains for coordinated extracellular signal-regulated kinase 5 and c-Jun N-terminal kinase signaling
    Kazuhiro Nakamura
    Department of Pharmacology, 1108 Mary Ellen Jones Building, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7365, USA
    Mol Cell Biol 27:4566-77. 2007
    ..The findings define how the MEKK2 and MEK5 PB1 domains are uniquely used for differential binding of two mitogen-activated protein kinase kinases, MEK5 and MKK7, for the coordinated control of ERK5 and c-Jun N-terminal kinase activation...
  39. pmc PB1 domain-dependent signaling complex is required for extracellular signal-regulated kinase 5 activation
    Kazuhiro Nakamura
    Department of Pharmacology, CB 7365, 1108 Mary Ellen Jones Building, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7365, USA
    Mol Cell Biol 26:2065-79. 2006
    ..The MEK5 PB1 domain confers stringent MAP3K regulation of ERK5 relative to more promiscuous MAP3K control of ERK1/2, JNK, and p38...
  40. ncbi request reprint Kinetics of HL-60 cell entry to apoptosis during treatment with TNF-alpha or camptothecin assayed by the stathmo-apoptosis method
    Piotr Smolewski
    Brander Cancer Research Institute, New York Medical College, 19 Bradhurst Avenue, Suite 2400, Hawthorne, NY 10532, USA
    Cytometry 47:143-9. 2002
    ..The CAI was measured at different time points for up to 48 h by flow cytometry. Bivariate analysis of DNA content and cell labeling with FLICA was used to correlate apoptosis with the cell-cycle position...
  41. ncbi request reprint Detection of caspases activation in situ by fluorochrome-labeled inhibitors of caspases (FLICA)
    Zbigniew Darzynkiewicz
    Brander Cancer Research Institute, New York Medical College, Valhalla, NY, USA
    Methods Mol Biol 203:289-99. 2002
  42. pmc Reovirus-induced alteration in expression of apoptosis and DNA repair genes with potential roles in viral pathogenesis
    Roberta L Debiasi
    Department of Pediatrics, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    J Virol 77:8934-47. 2003
    ..This provides the first comparative analysis of altered gene expression after infection with viruses of differing apoptotic phenotypes and provides insight into pathogenic mechanisms of virus-induced disease...
  43. ncbi request reprint MAPK kinase kinases (MKKKs) as a target class for small-molecule inhibition to modulate signaling networks and gene expression
    Gary L Johnson
    Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, NC 27599 7365, USA
    Curr Opin Chem Biol 9:325-31. 2005
    ....
  44. ncbi request reprint Wiring diagrams of MAPK regulation by MEKK1, 2, and 3
    Mark T Uhlik
    Department of Pharmacology, University of North Carolina School of Medicine, 1108 Mary Ellen Jones Building, CB 7365, Chapel Hill, NC 27599, USA
    Biochem Cell Biol 82:658-63. 2004
    ..We propose that signal transduction network wiring diagrams are valuable tools for hypothesis building and filtering physiologically relevant phenotypic responses from less connected protein relations in the regulation of MAPK pathways...
  45. ncbi request reprint Structural and evolutionary division of phosphotyrosine binding (PTB) domains
    Mark T Uhlik
    Department of Pharmacology and University of North Carolina School of Medicine, 1108 Mary Ellen Jones Building, Campus Box 7365, Chapel Hill, NC 27599 7365, USA
    J Mol Biol 345:1-20. 2005
    ..The signaling complexes organized by PTB domain encoded proteins are largely unknown and represents an important challenge in systems biology for the future...
  46. ncbi request reprint Sequential activation of caspases and serine proteases (serpases) during apoptosis
    Jerzy Grabarek
    Brander Cancer Research Institute New York Medical College Valhalla, New York 10595, USA
    Cell Cycle 1:124-31. 2002
    ..Their apparent molecular weight (62-65 kD) suggests that they are novel enzymes...
  47. ncbi request reprint CP-64131, an aminobenzazepine with cytokine-like properties, stimulates human neutrophil functions through the p38-MAPK pathway
    Marsha S Anderson
    Bonfils Blood Center, 717 Yosemite Street, Denver, CO 80230, USA
    J Leukoc Biol 76:477-83. 2004
    ..CP has the ability to activate specific MAPK pathways in different cell types and should prove to be an effective agonist in combination with inhibitors to study biological responses regulated by MAPKs...
  48. ncbi request reprint The in vitro production and characterization of neutrophils from embryonic stem cells
    Jonathan G Lieber
    National Jewish Medical and Research Center, 1400 Jackson St, Denver, CO 80206, USA
    Blood 103:852-9. 2004
    ..During the differentiation of these ES-derived neutrophils, regional areas of neutrophil production can be identified that have been designated as neutrophil generating regions (NGRs)...
  49. doi request reprint Homogeneous time-resolved fluorescence resonance energy transfer assay for measurement of Phox/Bem1p (PB1) domain heterodimerization
    Kazuhiro Nakamura
    Department of Pharmacology and the Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7365, USA
    J Biomol Screen 13:396-405. 2008
    ..Disruption of PB1 domain interactions represents a novel approach for selectively regulating the ERK5 signaling pathway independent of kinase active site-directed adenosine triphosphate competitive inhibitors...