A W Johnson

Summary

Affiliation: University of Texas
Country: USA

Publications

  1. pmc Rat1p and Xrn1p are functionally interchangeable exoribonucleases that are restricted to and required in the nucleus and cytoplasm, respectively
    A W Johnson
    Department of Microbiology and Institute for Cellular and Molecular Biology, University of Texas at Austin, 78712 1095, USA
    Mol Cell Biol 17:6122-30. 1997
  2. pmc Ribosome stalk assembly requires the dual-specificity phosphatase Yvh1 for the exchange of Mrt4 with P0
    Kai Yin Lo
    Department of Chemistry and Biochemistry, Section of Molecular Genetics and Microbiology, The Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
    J Cell Biol 186:849-62. 2009
  3. pmc Integrity of the P-site is probed during maturation of the 60S ribosomal subunit
    Cyril Bussiere
    Section of Molecular Genetics and Microbiology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
    J Cell Biol 197:747-59. 2012
  4. pmc Characterization of the nuclear export adaptor protein Nmd3 in association with the 60S ribosomal subunit
    Jayati Sengupta
    Wadsworth Center, New York State Department of Health, Albany, NY 12201, USA
    J Cell Biol 189:1079-86. 2010
  5. pmc Rational extension of the ribosome biogenesis pathway using network-guided genetics
    Zhihua Li
    Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas, USA
    PLoS Biol 7:e1000213. 2009
  6. pmc Of blood, bones, and ribosomes: is Swachman-Diamond syndrome a ribosomopathy?
    Arlen W Johnson
    Section of Molecular Genetics and Microbiology, The University of Texas at Austin, Texas 78712, USA
    Genes Dev 25:898-900. 2011
  7. ncbi request reprint Nuclear export of ribosomal subunits
    Arlen W Johnson
    Section of Molecular Genetics and Microbiology and Institute for Cellular and Molecular Biology, University of Texas at Austin, 78712, USA
    Trends Biochem Sci 27:580-5. 2002
  8. pmc Nascent 60S ribosomal subunits enter the free pool bound by Nmd3p
    J H Ho
    Section of Molecular Genetics and Microbiology and the Institute for Cellular and Molecular Biology, The University of Texas at Austin, 78712 1095, USA
    RNA 6:1625-34. 2000
  9. pmc Nmd3p is a Crm1p-dependent adapter protein for nuclear export of the large ribosomal subunit
    J H Ho
    Section of Molecular Genetics and Microbiology and the Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712, USA
    J Cell Biol 151:1057-66. 2000
  10. pmc Saccharomyces cerevisiae RAI1 (YGL246c) is homologous to human DOM3Z and encodes a protein that binds the nuclear exoribonuclease Rat1p
    Y Xue
    Section of Molecular Genetics and Microbiology and Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712 1095, USA
    Mol Cell Biol 20:4006-15. 2000

Research Grants

Collaborators

Detail Information

Publications28

  1. pmc Rat1p and Xrn1p are functionally interchangeable exoribonucleases that are restricted to and required in the nucleus and cytoplasm, respectively
    A W Johnson
    Department of Microbiology and Institute for Cellular and Molecular Biology, University of Texas at Austin, 78712 1095, USA
    Mol Cell Biol 17:6122-30. 1997
    ..It is likely that the higher eukaryotic homologs of these proteins will function similarly in the cytoplasm and nucleus...
  2. pmc Ribosome stalk assembly requires the dual-specificity phosphatase Yvh1 for the exchange of Mrt4 with P0
    Kai Yin Lo
    Department of Chemistry and Biochemistry, Section of Molecular Genetics and Microbiology, The Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
    J Cell Biol 186:849-62. 2009
    ..The initial assembly of the ribosome with Mrt4 may provide functional compartmentalization of ribosome assembly in addition to the spatial separation afforded by the nuclear envelope...
  3. pmc Integrity of the P-site is probed during maturation of the 60S ribosomal subunit
    Cyril Bussiere
    Section of Molecular Genetics and Microbiology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
    J Cell Biol 197:747-59. 2012
    ..We propose that Efl1 promotes a functional check of the integrity of the 60S subunit before its first round of translation...
  4. pmc Characterization of the nuclear export adaptor protein Nmd3 in association with the 60S ribosomal subunit
    Jayati Sengupta
    Wadsworth Center, New York State Department of Health, Albany, NY 12201, USA
    J Cell Biol 189:1079-86. 2010
    ..This cryo-EM map is a first step toward a molecular understanding of the functional role and release mechanism of Nmd3...
  5. pmc Rational extension of the ribosome biogenesis pathway using network-guided genetics
    Zhihua Li
    Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas, USA
    PLoS Biol 7:e1000213. 2009
    ....
  6. pmc Of blood, bones, and ribosomes: is Swachman-Diamond syndrome a ribosomopathy?
    Arlen W Johnson
    Section of Molecular Genetics and Microbiology, The University of Texas at Austin, Texas 78712, USA
    Genes Dev 25:898-900. 2011
    ..917-929) establish that SBDS functions in ribosome synthesis by promoting the recycling of eukaryotic initiation factor 6 (eIF6) in a GTP-dependent manner. This work supports the idea that a ribosomopathy may underlie this syndrome...
  7. ncbi request reprint Nuclear export of ribosomal subunits
    Arlen W Johnson
    Section of Molecular Genetics and Microbiology and Institute for Cellular and Molecular Biology, University of Texas at Austin, 78712, USA
    Trends Biochem Sci 27:580-5. 2002
    ..This structural proofreading is extended into the cytoplasm, where it is believed that several energy-requiring steps release shuttling factors from the subunit, allowing it to function in translation...
  8. pmc Nascent 60S ribosomal subunits enter the free pool bound by Nmd3p
    J H Ho
    Section of Molecular Genetics and Microbiology and the Institute for Cellular and Molecular Biology, The University of Texas at Austin, 78712 1095, USA
    RNA 6:1625-34. 2000
    ..These results suggest that Nmd3p recruits nascent 60S subunits into the pool of free 60S subunits and exchanges on 60S subunits as they recycle during translation...
  9. pmc Nmd3p is a Crm1p-dependent adapter protein for nuclear export of the large ribosomal subunit
    J H Ho
    Section of Molecular Genetics and Microbiology and the Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712, USA
    J Cell Biol 151:1057-66. 2000
    ..These results identify Crm1p as the receptor for Nmd3p export. Thus, export of the 60S subunit is mediated by the adapter protein Nmd3p in a Crm1p-dependent pathway...
  10. pmc Saccharomyces cerevisiae RAI1 (YGL246c) is homologous to human DOM3Z and encodes a protein that binds the nuclear exoribonuclease Rat1p
    Y Xue
    Section of Molecular Genetics and Microbiology and Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712 1095, USA
    Mol Cell Biol 20:4006-15. 2000
    ..Thus, deletion of RAI1 affects both 5' and 3' processing reactions of 5.8S rRNA. Consistent with the in vivo data suggesting that RAI1 enhances RAT1 function, purified Rai1p stabilized the in vitro exoribonuclease activity of Rat1p...
  11. pmc NMD3 encodes an essential cytoplasmic protein required for stable 60S ribosomal subunits in Saccharomyces cerevisiae
    J H Ho
    Department of Microbiology and Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas, USA
    Mol Cell Biol 19:2389-99. 1999
    ..However, these proteins all have an amino-terminal domain containing four repeats of Cx2C, reminiscent of zinc-binding proteins, implicated in nucleic acid binding or protein oligomerization...
  12. pmc Mutational analysis of exoribonuclease I from Saccharomyces cerevisiae
    A M Page
    Department of Microbiology and the Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712 1095, USA
    Nucleic Acids Res 26:3707-16. 1998
    ....
  13. pmc A cis-acting element known to block 3' mRNA degradation enhances expression of polyA-minus mRNA in wild-type yeast cells and phenocopies a ski mutant
    J T Brown
    Department of Molecular Genetics and Microbiology and The Institute for Cellular and Molecular Biology, The University of Texas at Austin, 78712-1095, USA
    RNA 7:1566-77. 2001
    ..Furthermore, a polyA-minus mRNA is expressed in wild-type cells when terminated in an element known to inhibit 3' decay in cis...
  14. pmc Inhibition of mRNA turnover in yeast by an xrn1 mutation enhances the requirement for eIF4E binding to eIF4G and for proper capping of transcripts by Ceg1p
    J T Brown
    Section of Molecular Genetics and Microbiology and the Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712 1095, USA
    Genetics 155:31-42. 2000
    ....
  15. pmc The yeast antiviral proteins Ski2p, Ski3p, and Ski8p exist as a complex in vivo
    J T Brown
    Section of Molecular Genetics and Microbiology and the Institute for Cellular and Molecular Biology, The University of Texas at Austin, 78712 1095, USA
    RNA 6:449-57. 2000
    ..These data support the idea that Ski2p, Ski3p, and Ski8p function in the cytoplasm in a 3'-mRNA degradation pathway...
  16. pmc Nuclear recycling of the pre-60S ribosomal subunit-associated factor Arx1 depends on Rei1 in Saccharomyces cerevisiae
    Nai Jung Hung
    Section of Molecular Genetics and Microbiology and Institute for Cellular and Molecular Biology, 1 University Station, A5000, The University of Texas at Austin, Austin, Texas 78712 0162, USA
    Mol Cell Biol 26:3718-27. 2006
    ..Furthermore, these results suggest that Rei1 is needed for release of Arx1 from nascent 60S subunits after export to the cytoplasm but not for the subsequent nuclear import of Arx1...
  17. pmc Arx1 is a nuclear export receptor for the 60S ribosomal subunit in yeast
    Nai Jung Hung
    Section of Molecular Genetics and Microbiology and the Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
    Mol Biol Cell 19:735-44. 2008
    ..These results show that Arx1 can directly bridge the interaction between the pre-60S particle and the NPC and thus is a third export receptor for the 60S subunit in yeast...
  18. pmc Defining the pathway of cytoplasmic maturation of the 60S ribosomal subunit
    Kai Yin Lo
    Section of Molecular Genetics and Microbiology, University of Texas at Austin, Austin, TX 78712, USA
    Mol Cell 39:196-208. 2010
    ..Finally, the release of Tif6 is a prerequisite for the release of the nuclear export adaptor Nmd3. Establishing this pathway provides an important conceptual framework for understanding ribosome maturation...
  19. pmc Bud23 methylates G1575 of 18S rRNA and is required for efficient nuclear export of pre-40S subunits
    Joshua White
    Section of Molecular Genetics and Microbiology, 1 University Station, A5000, The University of Texas at Austin, Austin, TX 78712 0162, USA
    Mol Cell Biol 28:3151-61. 2008
    ..Thus, Bud23 protein, but not its methyltransferase activity, is important for biogenesis and export of the 40S subunit in yeast...
  20. ncbi request reprint Mutational analysis of the ribosomal protein Rpl10 from yeast
    Anne Hofer
    Section of Molecular Genetics and Microbiology, Institute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station A5000, Austin, TX 78712, USA
    J Biol Chem 282:32630-9. 2007
    ..We also found that mutant Rpl10 proteins were engineered to be unable to bind to the ribosome accumulated in the nucleus. This was unexpected and may suggest a nuclear role for Rpl10...
  21. ncbi request reprint Novel interaction of the 60S ribosomal subunit export adapter Nmd3 at the nuclear pore complex
    Matthew West
    Section of Molecular Genetics and Microbiology and the Institute for Cellular and Molecular Biology, The University of Texas, Austin, TX 78712, USA
    J Biol Chem 282:14028-37. 2007
    ..These results suggest that in the absence of the ribosome, Nmd3 is not efficiently released from Crm1 at the NPC...
  22. pmc The specialized cytosolic J-protein, Jjj1, functions in 60S ribosomal subunit biogenesis
    Alison E Meyer
    Department of Biochemistry, 433 Babcock Drive, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 104:1558-63. 2007
    ....
  23. ncbi request reprint Mapping the functional domains of yeast NMD3, the nuclear export adapter for the 60 S ribosomal subunit
    John Hedges
    Section of Molecular Genetics and Microbiology and the Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78172 1095, USA
    J Biol Chem 281:36579-87. 2006
    ..We show that mutations in two regions of Nmd3p affect 60 S binding, suggesting that its binding to the subunit is multivalent...
  24. pmc Domain interactions within the Ski2/3/8 complex and between the Ski complex and Ski7p
    Lingna Wang
    Section of Molecular Genetics and Microbiology, The University of Texas, Austin, TX 78712 0162, USA
    RNA 11:1291-302. 2005
    ..In addition, inactivating the Ski complex by mutating conserved residues in the DEVH helicase motif of Ski2 did not abrogate its interaction with Ski7p, indicating that Ski2p function is not necessary for this interaction...
  25. pmc Defining the order in which Nmd3p and Rpl10p load onto nascent 60S ribosomal subunits
    Matthew West
    Section of Molecular Genetics and Microbiology, ESB 325, The University of Texas at Austin, Austin, TX 78712 1095, USA
    Mol Cell Biol 25:3802-13. 2005
    ..These results support the idea that Sqt1p loads Rpl10p onto the Nmd3p-bound subunit after export to the cytoplasm and that Rpl10p loading involves the GTPase Lsg1p...
  26. pmc Release of the export adapter, Nmd3p, from the 60S ribosomal subunit requires Rpl10p and the cytoplasmic GTPase Lsg1p
    John Hedges
    Section of Molecular Genetics and Microbiology, Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712 1095, USA
    EMBO J 24:567-79. 2005
    ..These results suggest a coupling between recycling the 60S export adapter and activation of 60S subunits for translation...
  27. pmc Coordinated nuclear export of 60S ribosomal subunits and NMD3 in vertebrates
    Christopher R Trotta
    Department of Biomolecular Chemistry, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706, USA
    EMBO J 22:2841-51. 2003
    ..We propose that NMD3 acts as an adaptor for CRM1-Ran.GTP-mediated 60S subunit export, by a mechanism that is conserved from vertebrates to yeast...
  28. pmc The role of Rat1 in coupling mRNA 3'-end processing to transcription termination: implications for a unified allosteric-torpedo model
    Weifei Luo
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, University of Colorado Health Sciences Center at Fitzsimons, Aurora, Colorado 80045, USA
    Genes Dev 20:954-65. 2006
    ..Our results suggest a unified allosteric/torpedo model in which Rat1 is not a dedicated termination factor, but is an integrated component of the cleavage/polyadenylation apparatus...

Research Grants15

  1. Export and Maturation of Ribosomal Subunits
    Arlen W Johnson; Fiscal Year: 2010
    ..Understanding these pathways and how they are integrated with other cellular pathways will provide the intellectual underpinning for investigators carrying out translational research. ..
  2. MATURATION OF THE LARGE RIBOSOMAL SUBUNIT IN YEAST
    Arlen Johnson; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  3. MATURATION OF THE LARGE RIBOSOMAL SUBUNIT IN YEAST
    Arlen Johnson; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  4. MATURATION OF THE LARGE RIBOSOMAL SUBUNIT IN YEAST
    Arlen Johnson; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  5. MATURATION OF THE LARGE RIBOSOMAL SUBUNIT IN YEAST
    Arlen Johnson; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  6. MATURATION OF THE LARGE RIBOSOMAL SUBUNIT IN YEAST
    Arlen Johnson; Fiscal Year: 2005
    ..3) Determine the function of Arx1 p and Reilp, two functionally related proteins that act at the polypeptide exit tunnel on the nascent large ribosomal subunit during export and as it enters the cytoplasmic pool of active subunits. ..
  7. MATURATION OF THE LARGE RIBOSOMAL SUBUNIT IN YEAST
    Arlen Johnson; Fiscal Year: 2004
    ..Two models accounting for these results are that this complex is required for normal 60S function or that this complex acts in a 3'-mRNA degradation pathway. Experiments will be carried out to distinguish between these two models. ..
  8. MATURATION OF THE LARGE RIBOSOMAL SUBUNIT IN YEAST
    Arlen Johnson; Fiscal Year: 2003
    ..Two models accounting for these results are that this complex is required for normal 60S function or that this complex acts in a 3'-mRNA degradation pathway. Experiments will be carried out to distinguish between these two models. ..
  9. MATURATION OF THE LARGE RIBOSOMAL SUBUNIT IN YEAST
    Arlen Johnson; Fiscal Year: 2002
    ..Two models accounting for these results are that this complex is required for normal 60S function or that this complex acts in a 3'-mRNA degradation pathway. Experiments will be carried out to distinguish between these two models. ..
  10. MATURATION OF THE LARGE RIBOSOMAL SUBUNIT IN YEAST
    Arlen Johnson; Fiscal Year: 2001
    ..Two models accounting for these results are that this complex is required for normal 60S function or that this complex acts in a 3'-mRNA degradation pathway. Experiments will be carried out to distinguish between these two models. ..
  11. MRNA DEGRADATION AND TRANSLATION CONTROL IN YEAST
    Arlen Johnson; Fiscal Year: 1999
    ..Many proteins involved in RNA turnover and translation control appear highly conserved among eukaryotes and are thought to help the cell respond to such challenges as pathogenic infection and oncogenesis. ..
  12. MATURATION OF THE LARGE RIBOSOMAL SUBUNIT IN YEAST
    Arlen Johnson; Fiscal Year: 2009
    ..3) Determine the function of Arx1 p and Reilp, two functionally related proteins that act at the polypeptide exit tunnel on the nascent large ribosomal subunit during export and as it enters the cytoplasmic pool of active subunits. ..