Lily I Jiang

Summary

Affiliation: University of Texas Southwestern Medical Center
Country: USA

Publications

  1. ncbi Mapping the Galpha13 binding interface of the rgRGS domain of p115RhoGEF
    Zhe Chen
    Department of Biochemistry, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 278:9912-9. 2003
  2. pmc Zymosan activates protein kinase A via adenylyl cyclase VII to modulate innate immune responses during inflammation
    Lily I Jiang
    Department of Pharmacology, UT Southwestern Medical Center, Dallas, TX 75390 9041, United States
    Mol Immunol 54:14-22. 2013
  3. pmc Use of a cAMP BRET sensor to characterize a novel regulation of cAMP by the sphingosine 1-phosphate/G13 pathway
    Lily I Jiang
    Department of Pharmacology, Alliance for Cellular Signaling, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9196, USA
    J Biol Chem 282:10576-84. 2007
  4. pmc Regulation of cAMP responses by the G12/13 pathway converges on adenylyl cyclase VII
    Lily I Jiang
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 283:23429-39. 2008
  5. pmc Regions on adenylyl cyclase VII required for selective regulation by the G13 pathway
    Lily I Jiang
    Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, Texas, USA 74390 9041, USA
    Mol Pharmacol 83:587-93. 2013
  6. pmc Distinct roles of adenylyl cyclase VII in regulating the immune responses in mice
    Biyan Duan
    Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Immunol 185:335-44. 2010
  7. pmc Modulation of a GEF switch: autoinhibition of the intrinsic guanine nucleotide exchange activity of p115-RhoGEF
    Zhe Chen
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Protein Sci 20:107-17. 2011
  8. pmc Activated RhoA binds to the pleckstrin homology (PH) domain of PDZ-RhoGEF, a potential site for autoregulation
    Zhe Chen
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Biol Chem 285:21070-81. 2010
  9. ncbi Functional characterization of p115 RhoGEF
    Clark Wells
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Methods Enzymol 345:371-82. 2002
  10. ncbi Structure of the p115RhoGEF rgRGS domain-Galpha13/i1 chimera complex suggests convergent evolution of a GTPase activator
    Zhe Chen
    Department of Biochemistry, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Nat Struct Mol Biol 12:191-7. 2005

Collaborators

  • Zhe Chen
  • S R Sprang
  • Melanie Cobb
  • Min Liu
  • Qin Wang
  • Robert C Hsueh
  • Robert A Rebres
  • Rama Ranganathan
  • Xiaocui Zhu
  • Tamara I A Roach
  • Elliott M Ross
  • Paul C Sternweis
  • Stephen Gutowski
  • Biyan Duan
  • Estelle A Wall
  • Xuejun Jiang
  • Clark Wells
  • Madhusudan Natarajan
  • William D Singer
  • Ewen D Gallagher
  • Lori B Christerson
  • Clark D Wells
  • Robert E Palmer
  • Eva L Sadat
  • Richard Davis
  • Dorothy Yuan
  • Julie Collins
  • Joelle R Zavzavadjian
  • Melvin I Simon
  • Xiaoyan Robert Bao
  • Mi Sook Chang
  • Jamie Liu
  • Baljinder Randhawa
  • Iain D C Fraser
  • Adrienne Driver
  • Ying Fan Hsiung
  • Angela M Carter
  • Shahab M Danesh
  • Keng Mean Lin
  • Takao Inoue
  • Pamela M Sternweis
  • Colleen A Vanderbilt
  • Paul W Sternberg
  • Tohru Kozasa
  • Roxana Kazempour
  • David R Sherwood
  • Mandy Jackson
  • Jeffrey D Rothstein
  • Angelique W Whitehurst
  • Ewen Gallagher

Detail Information

Publications23

  1. ncbi Mapping the Galpha13 binding interface of the rgRGS domain of p115RhoGEF
    Zhe Chen
    Department of Biochemistry, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 278:9912-9. 2003
    ..This suggests that the rgRGS domain may serve a structural or allosteric role in the regulation of the nucleotide exchange activity of p115RhoGEF on Rho by Galpha(13)...
  2. pmc Zymosan activates protein kinase A via adenylyl cyclase VII to modulate innate immune responses during inflammation
    Lily I Jiang
    Department of Pharmacology, UT Southwestern Medical Center, Dallas, TX 75390 9041, United States
    Mol Immunol 54:14-22. 2013
    ..This would also help explain a well documented phenomenon, that the ability of zymosan to stimulate inflammatory cytokine responses via TLR2 appears to be weaker than other stimuli of TLR2...
  3. pmc Use of a cAMP BRET sensor to characterize a novel regulation of cAMP by the sphingosine 1-phosphate/G13 pathway
    Lily I Jiang
    Department of Pharmacology, Alliance for Cellular Signaling, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9196, USA
    J Biol Chem 282:10576-84. 2007
    ..Thus in these macrophage cells, all four major classes of G proteins can regulate intracellular cAMP...
  4. pmc Regulation of cAMP responses by the G12/13 pathway converges on adenylyl cyclase VII
    Lily I Jiang
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 283:23429-39. 2008
    ..Our results demonstrate that AC7 is a specific downstream effector of the G(12/13) pathway...
  5. pmc Regions on adenylyl cyclase VII required for selective regulation by the G13 pathway
    Lily I Jiang
    Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, Texas, USA 74390 9041, USA
    Mol Pharmacol 83:587-93. 2013
    ..A mutagenesis screen identified more specific regions of AC7 that differentially mediate its regulation by distinct pathways...
  6. pmc Distinct roles of adenylyl cyclase VII in regulating the immune responses in mice
    Biyan Duan
    Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Immunol 185:335-44. 2010
    ..These results are the first to ascribe specific functions to an AC isoform in the immune system and emphasize the importance of cAMP synthesis by this isoform in shaping the immune responses...
  7. pmc Modulation of a GEF switch: autoinhibition of the intrinsic guanine nucleotide exchange activity of p115-RhoGEF
    Zhe Chen
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Protein Sci 20:107-17. 2011
    ..It is possible that Gα subunits activate p115 in part by relieving autoinhibition imposed by the linker region...
  8. pmc Activated RhoA binds to the pleckstrin homology (PH) domain of PDZ-RhoGEF, a potential site for autoregulation
    Zhe Chen
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Biol Chem 285:21070-81. 2010
    ..PH bound to both forms of RhoA can be isolated by size-exclusion chromatography. This novel interaction between activated RhoA and PH could play a key role in regulation of RhoGEF activity in vivo...
  9. ncbi Functional characterization of p115 RhoGEF
    Clark Wells
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Methods Enzymol 345:371-82. 2002
  10. ncbi Structure of the p115RhoGEF rgRGS domain-Galpha13/i1 chimera complex suggests convergent evolution of a GTPase activator
    Zhe Chen
    Department of Biochemistry, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Nat Struct Mol Biol 12:191-7. 2005
    ..The interface between the RGS module of rgRGS and Galpha is similar to that of a Galpha-effector complex, suggesting a role for the rgRGS domain in the stimulation of the GEF activity of p115RhoGEF by Galpha13...
  11. pmc Deciphering signaling outcomes from a system of complex networks
    Robert C Hsueh
    University of Texas Southwestern Medical Center, Dallas, TX 75390 9041, USA
    Sci Signal 2:ra22. 2009
    ..This suggests that most potential interactions are not realized and that unique cellular responses are limited to discrete subsets of ligands and pathways that enhance specific cellular functions...
  12. pmc Signaling and cross-talk by C5a and UDP in macrophages selectively use PLCbeta3 to regulate intracellular free calcium
    Tamara I A Roach
    Alliance for Cellular Signaling, Northern California Institute for Research and Education, San Francisco, California 94121, USA
    J Biol Chem 283:17351-61. 2008
    ..PLCbeta3 may thus provide a selective target for inhibiting Ca(2+) responses to mediators of inflammation, including C5a, UDP, PAF, and LPA...
  13. ncbi A global analysis of cross-talk in a mammalian cellular signalling network
    Madhusudan Natarajan
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9050, USA
    Nat Cell Biol 8:571-80. 2006
    ..A global analysis of cross-talk suggests that many external stimuli converge on a relatively small number of interaction mechanisms to provide for context-dependent signalling...
  14. ncbi Ribozyme- and siRNA-mediated suppression of RGS-containing RhoGEF proteins
    Qin Wang
    Department of Pharmacology, University of Michigan, Ann Arbor 48109, USA
    Methods Enzymol 389:244-65. 2004
    ..Also, the three siRNAs targeting LARG, PDZ-RhoGEF, and p115-RhoGEF are able to discriminate the closely related sequences within this RGS-RhoGEF gene family...
  15. ncbi Thrombin and lysophosphatidic acid receptors utilize distinct rhoGEFs in prostate cancer cells
    Qin Wang
    Department of Pharmacology, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Biol Chem 279:28831-4. 2004
    ..Suppression of p115rhoGEF had no effect. Thus different rhoGEFs (LARG and PDZrhoGEF) mediate downstream rho signaling by the thrombin and LPA receptors...
  16. pmc Suppression of LPS-induced TNF-alpha production in macrophages by cAMP is mediated by PKA-AKAP95-p105
    Estelle A Wall
    Alliance for Cellular Signaling, Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Sci Signal 2:ra28. 2009
    ..These data suggest that crosstalk between the TLR4 and cAMP pathways in macrophages can be coordinated through PKA-dependent scaffolds that localize specific pools of the kinase to distinct substrates...
  17. ncbi Mechanisms for reversible regulation between G13 and Rho exchange factors
    Clark D Wells
    Department of Pharmacology, University of Texas, Southwestern Medical Center at Dallas, Texas 75390 9041, USA
    J Biol Chem 277:1174-81. 2002
    ..These results suggest a mechanism for activation of the nucleotide exchange activity of p115 RhoGEF that involves direct and coordinate interaction of Galpha(13) to both its RGS and DH domains...
  18. pmc Recognition of the activated states of Galpha13 by the rgRGS domain of PDZRhoGEF
    Zhe Chen
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Structure 16:1532-43. 2008
    ..Replacement of key residues in this motif with their counterparts in p115RhoGEF confers GAP activity...
  19. ncbi Assays and characterization of mammalian phosphatidylinositol 4,5-bisphosphate-sensitive phospholipase D
    Xuejun Jiang
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Methods Enzymol 345:328-34. 2002
  20. ncbi Regulation of Rho guanine nucleotide exchange factors by G proteins
    Paul C Sternweis
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Adv Protein Chem 74:189-228. 2007
    ..An overview of identified interactions with other signaling proteins suggests the growing spectrum of their involvement in numerous signaling pathways...
  21. ncbi p115 Rho GTPase activating protein interacts with MEKK1
    Lori B Christerson
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    J Cell Physiol 192:200-8. 2002
    ..Here we have identified an MEKK1 binding partner that offers a connection between this protein kinase and the machinery regulating cytoskeletal reorganization...
  22. ncbi RhoA binds to the amino terminus of MEKK1 and regulates its kinase activity
    Ewen D Gallagher
    Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9041, USA
    J Biol Chem 279:1872-7. 2004
    ..In summary, we have characterized a novel point at which Rho GTPases impinge upon the regulation and function of MEKK1...
  23. ncbi Caenorhabditis elegans cog-1 locus encodes GTX/Nkx6.1 homeodomain proteins and regulates multiple aspects of reproductive system development
    Robert E Palmer
    Howard Hughes Medical Institute and Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Dev Biol 252:202-13. 2002
    ..Two mutant alleles of cog-1 differentially affect alternative transcripts and cause different phenotypes, suggesting that the two forms of cog-1 have distinct functions in C. elegans...

Research Grants3

  1. Regulation of adenylyl cyclase VII and its function in the immune system
    Paul C Sternweis; Fiscal Year: 2010
    ..Results from these studies will help identify novel targets for selective therapeutic intervention in immune diseases. ..
  2. Regulation of adenylyl cyclase VII and its function in the immune system
    Li Jiang; Fiscal Year: 2009
    ..Results from these studies will help identify novel targets for selective therapeutic intervention in immune diseases. ..