Research Topics
| Michael JenningsSummaryAffiliation: University of Arkansas for Medical Sciences Country: USA Publications
Research Grants
| Collaborators
|
Detail Information
Publications
Inactivation of Saccharomyces cerevisiae sulfate transporter Sul2p: use it and lose itMichael L Jennings
Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA
Biophys J 102:768-76. 2012..02 mM SO(4)(=) to S-starved met3Δ cells. This transient efflux provides further protection against excessive SO(4)(=) influx and may represent an alternate transport mode of Sul2p...- Chloride homeostasis in Saccharomyces cerevisiae: high affinity influx, V-ATPase-dependent sequestration, and identification of a candidate Cl- sensorMichael L Jennings
Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
J Gen Physiol 131:379-91. 2008..This is the first example of a biological system that can regulate cellular Cl- at concentrations far below 1 mM... 
Direct estimate of 1:1 stoichiometry of K(+)-Cl(-) cotransport in rabbit erythrocytesM L Jennings
Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
Am J Physiol Cell Physiol 281:C825-32. 2001..The ratio of the NEM-stimulated K(+) to Cl(-) efflux is 1.12 +/- 0.26 (SD, n = 5). We conclude that K(+)-Cl(-) cotransport in rabbit red blood cells has a stoichiometry of 1:1...- Transport and regulatory characteristics of the yeast bicarbonate transporter homolog Bor1pMichael L Jennings
Dept of Physiology and Biophysics, University of Arkansas for Medical Sciences, 4301 W Markham St, Mail Slot 505, Little Rock, AR 72205, USA
Am J Physiol Cell Physiol 293:C468-76. 2007..A possible role in vacuolar dynamics for Bor1p was recently reported by Decker and Wickner. Under the conditions used presently, there appears to be mildly abnormal vacuolar morphology in the deletant strain... - Evidence for a second binding/transport site for chloride in erythrocyte anion transporter AE1 modified at glutamate 681Michael L Jennings
Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
Biophys J 88:2681-91. 2005..The effects of graded modification of E681 or inhibition by H(2)DIDS are consistent with the idea that the new Cl(-) binding site is on the same E681OH-modified subunit of the AE1 dimer as the normal transport site... - Pre-steady state transport by erythrocyte band 3 protein: uphill countertransport induced by the impermeant inhibitor H2DIDSM L Jennings
Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock 72205, USA
Biochem Cell Biol 76:807-13. 1998..These results are completely consistent with a ping-pong mechanism of anion exchange in which the catalytic cycle consists of one pair of exchanging anions per subunit of the band 3 dimer... - Volume-sensitive K(+)/Cl(-) cotransport in rabbit erythrocytes. Analysis of the rate-limiting activation and inactivation eventsM L Jennings
Department of Physiology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
J Gen Physiol 114:743-58. 1999..The rate-limiting inactivation event may be mediated by a protein kinase that is inhibited, either directly or indirectly, by cell swelling, low Mg(2+), acid pH, and NEM... - Topology of the anion exchange protein AE1: the controversial sidedness of lysine 743Hiroyuki Kuma
Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, 4301 West Markham Street, Little Rock, AR 72205, USA
Biochemistry 41:3380-8. 2002..Therefore, the actions of trypsin on intact cells, resealed ghosts, unsealed ghosts, inside-out vesicles, and microsomes from HEK293 cells all indicate that K743 is cytoplasmic and not extracellular... - Mouse Ae1 E699Q mediates SO42-i/anion-o exchange with [SO42-]i-dependent reversal of wild-type pHo sensitivityMarina N Chernova
Molecular and Vascular Medicine Unit, Beth Israel Deaconess Med Ctr, 330 Brookline Ave, Boston, MA 02215, USA
Am J Physiol Cell Physiol 295:C302-12. 2008.... - Aquaporin-2: COOH terminus is necessary but not sufficient for routing to the apical membranePeter M T Deen
Department of Cell Physiology, University Medical Center St Radboud, Nijmegen 6500 HB, The Netherlands
Am J Physiol Renal Physiol 282:F330-40. 2002..The study shows that the AQP2 COOH tail is necessary but not sufficient for routing to the AM and suggests that other parts of AQP2 are needed for AQP2 accumulation in intracellular vesicles... - Biotin dependency due to a defect in biotin transportRebecca Mardach
Regional Metabolic Service, Kaiser Permanente, Los Angeles, California, USA
J Clin Invest 109:1617-23. 2002..These results provide evidence for a novel genetic defect in biotin transport. This child is the first known with this defect, which should now be included in the identified causes of biotin dependency...
Research Grants
- MECHANISMS OF SULFATE TRANSPORT AND REGULATIONMichael Jennings; Fiscal Year: 2007..abstract_text> ..
- BIOCHEMICAL STUDIES OF ERYTHROCYTE ANION EXCHANGEMichael Jennings; Fiscal Year: 2001..The mutagenesis will be performed in band 3 expressed in both Xenopus oocytes and in yeast. ..
- MECHANISMS OF SULFATE TRANSPORT AND REGULATIONMichael Jennings; Fiscal Year: 2007..These experiments will provide new insights into the regulation of a basic pathway (SO4 = supply to sulfotransferases) that is of importance not only in the skeletal system but in many branches of human pathophysiology. ..