Paul Janmey

Summary

Affiliation: University of Pennsylvania
Country: USA

Publications

  1. doi request reprint Cytoplasmic transport: bacteria turn to glass unless kicked
    Paul A Janmey
    Departments of Physiology and Physics and Astronomy, Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA 19104, USA Department of Physics and Astronomy, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands Electronic address
    Curr Biol 24:R226-8. 2014
  2. doi request reprint From tissue mechanics to transcription factors
    Paul A Janmey
    Departments of Physiology and Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA 19104, USA Electronic address
    Differentiation 86:112-20. 2013
  3. pmc Hypogelsolinemia, a disorder of the extracellular actin scavenger system, in patients with multiple sclerosis
    Alina Kułakowska
    Department of Neurology, Medical University of Białystok, 15 230 Białystok, Poland
    BMC Neurol 10:107. 2010
  4. pmc Bactericidal activities of the cationic steroid CSA-13 and the cathelicidin peptide LL-37 against Helicobacter pylori in simulated gastric juice
    Katarzyna Leszczynska
    Department of Diagnostic Microbiology, Medical University of Bialystok, 15 230 Bialystok, Poland
    BMC Microbiol 9:187. 2009
  5. pmc The two neutrophil members of the formylpeptide receptor family activate the NADPH-oxidase through signals that differ in sensitivity to a gelsolin derived phosphoinositide-binding peptide
    Huamei Fu
    Department of Rheumatology and Inflammation Research, University of Goteborg, Sweden
    BMC Cell Biol 5:50. 2004
  6. ncbi request reprint Biophysical properties of lipids and dynamic membranes
    P A Janmey
    Institute for Medicine and Engineering, Department of Physiology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Trends Cell Biol 16:538-46. 2006
  7. ncbi request reprint Dealing with mechanics: mechanisms of force transduction in cells
    Paul A Janmey
    Departments of Physiology and Physics, University of Pennsylvania, Institute for Medicine and Engineering, 3340 Smith Walk, Philadelphia, PA 19104, USA
    Trends Biochem Sci 29:364-70. 2004
  8. pmc Fibrin gels and their clinical and bioengineering applications
    Paul A Janmey
    Department of Physiology, Institute for Medicine and Engineering, University of Pennsylvania, 3340 Smith Walk, Philadelphia, PA 19104, USA
    J R Soc Interface 6:1-10. 2009
  9. ncbi request reprint Cytoskeletal regulation: rich in lipids
    Paul A Janmey
    Institute for Medicine and Engineering, University of Pennsylvania, 1010 Vagelos Laboratories, 3340 Smith Walk, Philadelphia, Pennsylvania 19104, USA
    Nat Rev Mol Cell Biol 5:658-66. 2004
  10. ncbi request reprint Negative normal stress in semiflexible biopolymer gels
    Paul A Janmey
    Institute for Medicine and Engineering, University of Pennsylvania, 3340 Smith Walk, Philadelphia, Pennsylvania 19104, USA
    Nat Mater 6:48-51. 2007

Collaborators

Detail Information

Publications74

  1. doi request reprint Cytoplasmic transport: bacteria turn to glass unless kicked
    Paul A Janmey
    Departments of Physiology and Physics and Astronomy, Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA 19104, USA Department of Physics and Astronomy, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands Electronic address
    Curr Biol 24:R226-8. 2014
    ..New data show that bacterial cytoplasm can solidify to resemble a soft glass, unless enzymatic activity creates motions to fluidize it. ..
  2. doi request reprint From tissue mechanics to transcription factors
    Paul A Janmey
    Departments of Physiology and Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA 19104, USA Electronic address
    Differentiation 86:112-20. 2013
    ....
  3. pmc Hypogelsolinemia, a disorder of the extracellular actin scavenger system, in patients with multiple sclerosis
    Alina Kułakowska
    Department of Neurology, Medical University of Białystok, 15 230 Białystok, Poland
    BMC Neurol 10:107. 2010
    ....
  4. pmc Bactericidal activities of the cationic steroid CSA-13 and the cathelicidin peptide LL-37 against Helicobacter pylori in simulated gastric juice
    Katarzyna Leszczynska
    Department of Diagnostic Microbiology, Medical University of Bialystok, 15 230 Bialystok, Poland
    BMC Microbiol 9:187. 2009
    ..pylori potential of cathelicidin LL-37 peptide, which is naturally present in gastric juice, its optimized synthetic analog WLBU2, and the non-peptide antibacterial agent ceragenin CSA-13...
  5. pmc The two neutrophil members of the formylpeptide receptor family activate the NADPH-oxidase through signals that differ in sensitivity to a gelsolin derived phosphoinositide-binding peptide
    Huamei Fu
    Department of Rheumatology and Inflammation Research, University of Goteborg, Sweden
    BMC Cell Biol 5:50. 2004
    ....
  6. ncbi request reprint Biophysical properties of lipids and dynamic membranes
    P A Janmey
    Institute for Medicine and Engineering, Department of Physiology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Trends Cell Biol 16:538-46. 2006
    ..Here, we highlight some recent reports that the physical properties of the membrane can help control the function of transmembrane proteins and the motor-dependent elongation of internal organelles, such as the endoplasmic reticulum...
  7. ncbi request reprint Dealing with mechanics: mechanisms of force transduction in cells
    Paul A Janmey
    Departments of Physiology and Physics, University of Pennsylvania, Institute for Medicine and Engineering, 3340 Smith Walk, Philadelphia, PA 19104, USA
    Trends Biochem Sci 29:364-70. 2004
  8. pmc Fibrin gels and their clinical and bioengineering applications
    Paul A Janmey
    Department of Physiology, Institute for Medicine and Engineering, University of Pennsylvania, 3340 Smith Walk, Philadelphia, PA 19104, USA
    J R Soc Interface 6:1-10. 2009
    ..The biochemical and mechanical properties of fibrin have recently been exploited in numerous studies that suggest its potential for applications in medicine and bioengineering...
  9. ncbi request reprint Cytoskeletal regulation: rich in lipids
    Paul A Janmey
    Institute for Medicine and Engineering, University of Pennsylvania, 1010 Vagelos Laboratories, 3340 Smith Walk, Philadelphia, Pennsylvania 19104, USA
    Nat Rev Mol Cell Biol 5:658-66. 2004
    ....
  10. ncbi request reprint Negative normal stress in semiflexible biopolymer gels
    Paul A Janmey
    Institute for Medicine and Engineering, University of Pennsylvania, 3340 Smith Walk, Philadelphia, Pennsylvania 19104, USA
    Nat Mater 6:48-51. 2007
    ..We show that these negative normal stresses can be as large as the shear stress and that this property is directly related to the nonlinear strain-stiffening behaviour of biopolymer gels...
  11. ncbi request reprint Cell mechanics: integrating cell responses to mechanical stimuli
    Paul A Janmey
    Department of Physiology, Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Annu Rev Biomed Eng 9:1-34. 2007
    ....
  12. pmc Rheology
    Paul A Janmey
    Institute for Medicine and Engineering, University of Pennsylvania, 1010 Vagelos Laboratories, 3340 Smith Walk, Philadelphia, Pennsylvania 19104, USA
    Curr Biol 18:R639-R641. 2008
  13. ncbi request reprint The antimicrobial activity of the cathelicidin LL37 is inhibited by F-actin bundles and restored by gelsolin
    Daniel J Weiner
    Division of Pulmonary Medicine, The Children s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA
    Am J Respir Cell Mol Biol 28:738-45. 2003
    ..These findings may have therapeutic implications for treatments previously thought to alter only the viscoelastic properties of airway secretions and amplify the possible advantage of gelsolin in CF treatment...
  14. ncbi request reprint Inactivation of endotoxin by human plasma gelsolin
    Robert Bucki
    Department of Physiology and Institute for Medicine and Engineering, University of Pennsylvania, 1010 Vagelos Research Laboratories, 3340 Smith Walk, Philadelphia, Pennsylvania 19104, USA buckirob mail med upenn edu
    Biochemistry 44:9590-7. 2005
    ..These results show a strong effect of LPS on plasma gelsolin function and suggest that some effects of endotoxin in vivo may be mediated or inhibited by plasma gelsolin...
  15. pmc Biomechanics: cell research and applications for the next decade
    Dennis Discher
    University of Pennsylvania, Philadelphia, PA, USA
    Ann Biomed Eng 37:847-59. 2009
    ..Such insights will no doubt inform basic biology and rational engineering of effective new approaches to clinical therapy...
  16. pmc Novel cationic lipids with enhanced gene delivery and antimicrobial activity
    David E Fein
    Institute for Medicine and Engineering, Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Mol Pharmacol 78:402-10. 2010
    ..Mixtures of DS and D(2)S can improve transfection activity over common lipofection reagents, and D(2)S has strong antimicrobial action suited for the suppression of bacterial-mediated inflammation...
  17. pmc Matrices with compliance comparable to that of brain tissue select neuronal over glial growth in mixed cortical cultures
    Penelope C Georges
    Institute for Medicine and Engineering, Sea Run Holdings Inc, Freeport, Maine, USA
    Biophys J 90:3012-8. 2006
    ....
  18. ncbi request reprint Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion
    Tony Yeung
    Institute for Medicine and Engineering, University of Pennsylvania, PA 19104, USA
    Cell Motil Cytoskeleton 60:24-34. 2005
    ..These results support the hypothesis that mechanical factors impact different cell types in fundamentally different ways, and can trigger specific changes similar to those stimulated by soluble ligands...
  19. pmc Softness, strength and self-repair in intermediate filament networks
    Oliver I Wagner
    Department of Physiology, Institute for Medicine and Engineering, University of Pennsylvania, 1010 Vagelos Laboratories, 3340 Smith Walk, Philadelphia, PA 19104, USA
    Exp Cell Res 313:2228-35. 2007
    ..The polyelectrolyte properties of IFs may enable crossbridging by multivalent counterions, but identifying the mechanisms by which IFs link into bundles and networks in vivo remains a challenge...
  20. ncbi request reprint Domain unfolding in neurofilament sidearms: effects of phosphorylation and ATP
    Helim Aranda-Espinoza
    Institute for Medicine and Engineering, 1080 Vagelos Research Laboratory, 3340 Smith Walk, University of Pennsylvania, Philadelphia, PA 19104, USA
    FEBS Lett 531:397-401. 2002
    ..However, the native phosphorylated SA requires three-fold higher force to unfold by AFM than dephosphorylated SA, suggesting a less pliant as well as larger structure when phosphorylated...
  21. ncbi request reprint Structural and functional damage sustained by mitochondria after traumatic brain injury in the rat: evidence for differentially sensitive populations in the cortex and hippocampus
    Jonathan Lifshitz
    Head Injury Center, Department of Neurosurgery, University of Pensylvania, Philadelphia, Pensylvania 19104 6316, USA
    J Cereb Blood Flow Metab 23:219-31. 2003
    ..The heterogeneous mitochondrial response to TBI may underlie posttraumatic metabolic dysfunction and contribute to the pathophysiology of TBI...
  22. pmc Cathelicidin LL-37 peptide regulates endothelial cell stiffness and endothelial barrier permeability
    Fitzroy J Byfield
    Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, 19104, USA
    Am J Physiol Cell Physiol 300:C105-12. 2011
    ..Such a mechanism may help to maintain tissue fluid homeostasis...
  23. pmc Involvement of the Na+/H+ exchanger in membrane phosphatidylserine exposure during human platelet activation
    Robert Bucki
    University of Pennsylvania, Department of Physiology, Institute for Medicine and Engineering, 1010 Vagelos Research Laboratories, 3340 Smith Walk, Philadelphia, PA 19104, USA
    Biochim Biophys Acta 1761:195-204. 2006
    ..All these results would indicate that the mechanism that results in PS exposure uses redundant pathways inextricably linked to the physio-pathological requirements of this process...
  24. pmc Plasma gelsolin modulates cellular response to sphingosine 1-phosphate
    Robert Bucki
    Univ of Pennsylvania, Inst for Medicine and Engineering, 1010 Vagelos Research Laboratories, 3340 Smith Walk, Philadelphia, PA 19104, USA
    Am J Physiol Cell Physiol 299:C1516-23. 2010
    ..These findings suggest that gelsolin-mediated regulation of S1P bioactivity may be important to maintain immunomodulatory balance at inflammatory sites...
  25. ncbi request reprint Resistance of the antibacterial agent ceragenin CSA-13 to inactivation by DNA or F-actin and its activity in cystic fibrosis sputum
    Robert Bucki
    Department of Physiology and the Institute for Medicine and Engineering, 1010 Vagelos Research Laboratories, University of Pennsylvania, 3340 Smith Walk, Philadelphia, PA 19104, USA
    J Antimicrob Chemother 60:535-45. 2007
    ....
  26. pmc Salivary mucins inhibit antibacterial activity of the cathelicidin-derived LL-37 peptide but not the cationic steroid CSA-13
    Robert Bucki
    Department of Physiology, Institute for Medicine and Engineering, University of Pennsylvania, 1010 Vagelos Research Laboratories, 3340 Smith Walk, Philadelphia, PA 19104, USA
    J Antimicrob Chemother 62:329-35. 2008
    ....
  27. doi request reprint Bone marrow-derived human mesenchymal stem cells become quiescent on soft substrates but remain responsive to chemical or mechanical stimuli
    Jessamine P Winer
    Department of Bioengineering, Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Tissue Eng Part A 15:147-54. 2009
    ..These observations suggest that mechanical signals from the elasticity of the extracellular matrix may be one of the factors that enable the bone marrow niche to maintain MSCs as a reservoir for a long period...
  28. pmc Combined antibacterial and anti-inflammatory activity of a cationic disubstituted dexamethasone-spermine conjugate
    Robert Bucki
    Department of Physiology and the Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA 19104, USA
    Antimicrob Agents Chemother 54:2525-33. 2010
    ..The combined antibacterial and anti-inflammatory activities of D2S suggest its potential as an alternative to natural CAPs in the prevention and treatment of some bacterial infections...
  29. ncbi request reprint Extracellular gelsolin binds lipoteichoic acid and modulates cellular response to proinflammatory bacterial wall components
    Robert Bucki
    University of Pennsylvania, Institute for Medicine and Engineering, Philadelphia, PA 19104, USA
    J Immunol 181:4936-44. 2008
    ..These data suggest that extracellular gelsolin is involved in the host immune recognition of LTA or LPS following release of these molecules from the bacterial outer membrane during cell division or attack by drugs and immune components...
  30. pmc Non-linear elasticity of extracellular matrices enables contractile cells to communicate local position and orientation
    Jessamine P Winer
    Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
    PLoS ONE 4:e6382. 2009
    ..Thus cells are acutely responsive to the nonlinear elasticity of their substrates and can manipulate this rheological property to induce patterning...
  31. ncbi request reprint Plasma gelsolin: function, prognostic value, and potential therapeutic use
    Robert Bucki
    University of Pennsylvania, Institute for Medicine and Engineering, Vagelos Research Laboratories, Philadelphia, PA 19104, USA
    Curr Protein Pept Sci 9:541-51. 2008
    ..This review summarizes current data supporting a role of plasma gelsolin in extracellular fluids and the potential for its use as a diagnostic marker or therapeutic treatment in several medical conditions...
  32. pmc Interaction of the gelsolin-derived antibacterial PBP 10 peptide with lipid bilayers and cell membranes
    Robert Bucki
    Department of Physiology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Antimicrob Agents Chemother 50:2932-40. 2006
    ....
  33. pmc Antibacterial activities of rhodamine B-conjugated gelsolin-derived peptides compared to those of the antimicrobial peptides cathelicidin LL37, magainin II, and melittin
    Robert Bucki
    Department of Physiology and the Institute for Medicine and Engineering, Vagelos Research Laboratories, University of Pennsylvania, Philadelphia 19104, USA
    Antimicrob Agents Chemother 48:1526-33. 2004
    ....
  34. pmc Visualizing the Ca2+-dependent activation of gelsolin by using synchrotron footprinting
    Janna G Kiselar
    Department of Physiology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461 1602, USA
    Proc Natl Acad Sci U S A 100:3942-7. 2003
    ....
  35. pmc Nonlinear elasticity of stiff filament networks: strain stiffening, negative normal stress, and filament alignment in fibrin gels
    Hyeran Kang
    Department of Physics and Astronomy, University of Pennsylvania, PA, USA
    J Phys Chem B 113:3799-805. 2009
    ....
  36. ncbi request reprint Nonlinear elasticity in biological gels
    Cornelis Storm
    Department of Physics and Astronomy, University of Pennsylvania, 209 South 33rd Street, Philadelphia, Pennsylvania 19104, USA
    Nature 435:191-4. 2005
    ..This theory shows that systems of filamentous proteins arranged in an open crosslinked mesh invariably stiffen at low strains without requiring a specific architecture or multiple elements with different intrinsic stiffness...
  37. pmc Separation of insulin signaling into distinct GLUT4 translocation and activation steps
    Makoto Funaki
    Department of Physiology, Institute for Medicine and Engineering, University of Pennsylvania, 1080 Vagelos Research Laboratories, 3340 Smith Walk, Philadelphia, PA 19104, USA
    Mol Cell Biol 24:7567-77. 2004
    ..These results suggest that GLUT4 requires translocation to the plasma membrane, as well as activation at the plasma membrane, to initiate glucose uptake, and both of these steps normally require PI 3-kinase activation...
  38. pmc PI 4,5-P2 stimulates glucose transport activity of GLUT4 in the plasma membrane of 3T3-L1 adipocytes
    Makoto Funaki
    Department of Physiology, Institute for Medicine and Engineering, University of Pennsylvania, 1080 Vagelos Research Laboratories, 3340 Smith Walk, Philadelphia, 19104, USA
    Biochim Biophys Acta 1763:889-99. 2006
    ..These results suggest that PI 4,5-P2 in the plasma membrane acts as a second messenger to activate GLUT4, possibly through F-actin remodeling...
  39. ncbi request reprint Dissecting the gelsolin-polyphosphoinositide interaction and engineering of a polyphosphoinositide-sensitive gelsolin C-terminal half protein
    Wujing Xian
    Institute for Medicine and Engineering, University of Pennsylvania, 1010 Vagelos Laboratories, 3340 Smith Walk, Philadelphia 19104, USA
    J Mol Biol 322:755-71. 2002
    ..Furthermore, the P2 site in P2Ct and the actin-binding sites of Ct do not overlap, suggesting that PPIs regulate actin binding of P2Ct through induction of structural changes, rather than through direct competition...
  40. pmc Mechanisms of mechanical signaling in development and disease
    Paul A Janmey
    Institute for Medicine and Engineering, University of Pennsylvania, 1010 Vagelos Laboratories, 3340 Smith Walk, PA 19104, USA
    J Cell Sci 124:9-18. 2011
    ....
  41. ncbi request reprint Cell type-specific response to growth on soft materials
    Penelope C Georges
    Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Appl Physiol 98:1547-53. 2005
    ..We will attempt to link the mechanoresponse to real pathological states and speculate on the possible biological significance of mechanosensing...
  42. pmc Fibroblast adaptation and stiffness matching to soft elastic substrates
    Jerome Solon
    Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Biophys J 93:4453-61. 2007
    ....
  43. pmc Spotted vesicles, striped micelles and Janus assemblies induced by ligand binding
    David A Christian
    Laboratory for Research on the Structure of Matter, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Nat Mater 8:843-9. 2009
    ....
  44. ncbi request reprint Basic rheology for biologists
    Paul A Janmey
    Department of Bioengineering, Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Methods Cell Biol 83:3-27. 2007
    ..With proper choice of experimental setup, rheologic techniques can give valuable information about cellular systems and dynamics on a timescale that is closely related to biological functions...
  45. ncbi request reprint Increased stiffness of the rat liver precedes matrix deposition: implications for fibrosis
    Penelope C Georges
    Dept of Medicine, Univ of Pennsylvania School of Medicine, Philadelphia, PA 19104 6140, USA
    Am J Physiol Gastrointest Liver Physiol 293:G1147-54. 2007
    ..We suggest that increased liver stiffness may play an important role in initiating the early stages of fibrosis...
  46. ncbi request reprint Flavonoid-mediated inhibition of actin polymerization in cold-activated platelets
    Jennifer J Pastore
    Institute for Medicine and Engineering, University of Pennsylvania, 1010 Vagelos Research Laboratories, 3340 Smith Walk, Philadelphia, PA 19104, USA
    Platelets 16:362-7. 2005
    ..These data confirm the important links that exist between agonist- and cold-mediated platelet activation, suggesting a possible advantage of incorporating the use of flavonoids to allow platelet hypothermic-storage...
  47. ncbi request reprint Tissue cells feel and respond to the stiffness of their substrate
    Dennis E Discher
    School of Engineering and Applied Science and Cell and Molecular Biology Graduate Group, University of Pennsylvania, Philadelphia, PA 19104 6315, USA
    Science 310:1139-43. 2005
    ..The feedback of local matrix stiffness on cell state likely has important implications for development, differentiation, disease, and regeneration...
  48. pmc Spreading of neutrophils: from activation to migration
    Kheya Sengupta
    Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA, USA
    Biophys J 91:4638-48. 2006
    ..The different spreading regimes correspond to distinct values of the exponent b and are related to the adhesion state of the cell. Treatment with cytochalasin-B eliminated the anisotropy in the spreading...
  49. pmc Calcium-dependent lateral organization in phosphatidylinositol 4,5-bisphosphate (PIP2)- and cholesterol-containing monolayers
    Ilya Levental
    Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Biochemistry 48:8241-8. 2009
    ..This result emphasizes the likely critical role of subphase factors and lipid head-group specificity in the formation and stability of cholesterol-dependent domains in cellular plasma membranes...
  50. pmc The interaction of neurofilaments with the microtubule motor cytoplasmic dynein
    Oliver I Wagner
    Institute of Medicine and Engineering, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6085, USA
    Mol Biol Cell 15:5092-100. 2004
    ..Together these data suggest that a specific interaction between neurofilament subunit M and cytoplasmic dynein is involved in the saltatory bidirectional motility of neurofilaments undergoing axonal transport in the neuron...
  51. pmc Absence of filamin A prevents cells from responding to stiffness gradients on gels coated with collagen but not fibronectin
    Fitzroy J Byfield
    Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Biophys J 96:5095-102. 2009
    ..These results have implications for different models of cell protrusion and adhesion and identify a key role for filamin A in altering cellular stiffness that cannot be compensated for by other actin cross-linkers in vivo...
  52. pmc Interactions of membrane-active peptides with thick, neutral, nonzwitterionic bilayers
    Kandaswamy Vijayan
    Departments of Physics, of Chemical and Biomolecular Engineering, and of Physiology and Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Phys Chem B 109:14356-64. 2005
    ..We find that although alamethicin is too small to span the bilayer, the peptide interacts with, and ruptures, thick polymer membranes...
  53. pmc Mechanically induced reactive gliosis causes ATP-mediated alterations in astrocyte stiffness
    William J Miller
    Departments of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    J Neurotrauma 26:789-97. 2009
    ....
  54. pmc Cell-cycle control by physiological matrix elasticity and in vivo tissue stiffening
    Eric A Klein
    Department of Pharmacology, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Curr Biol 19:1511-8. 2009
    ..In this report, we use hydrogel-based substrata matched to biological tissue stiffness to investigate the effects of matrix elasticity on the cell cycle...
  55. pmc The hard life of soft cells
    Paul A Janmey
    Department of Physiology, Institute for Medicine and Engineering, University of Pennsylvania, 1010 Vagelos Laboratories, Philadelphia, Pennsylvania, USA
    Cell Motil Cytoskeleton 66:597-605. 2009
    ..Cell Motil. Cytoskeleton, 2009. (c) 2009 Wiley-Liss, Inc...
  56. ncbi request reprint Phosphoinositide regulation of the actin cytoskeleton
    Helen L Yin
    Department of Physiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Annu Rev Physiol 65:761-89. 2003
    ....
  57. pmc Combined electrostatics and hydrogen bonding determine intermolecular interactions between polyphosphoinositides
    Ilya Levental
    Institute for Medicine and Engineering, Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Am Chem Soc 130:9025-30. 2008
    ....
  58. pmc Cholesterol-dependent phase separation in cell-derived giant plasma-membrane vesicles
    Ilya Levental
    Department of Bioengineering, University of Pennsylvania, 1010 Vagelos Laboratories, 3340 Smith Walk, Philadelphia, PA 19104, USA
    Biochem J 424:163-7. 2009
    ..The results of the present study extend previous observations in purified lipid systems to the complex environment of the plasma membrane and provide insight into the effect of cholesterol on lipid phase separation and abundance...
  59. pmc Delayed loss of control of plasma lipopolysaccharide levels after therapy interruption in chronically HIV-1-infected patients
    Emmanouil Papasavvas
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    AIDS 23:369-75. 2009
    ..Increased circulating levels of lipopolysaccharide (LPS) have been demonstrated in HIV-1-infected progressors. We investigated the effect of antiretroviral therapy (ART) interruptions on plasma LPS levels...
  60. doi request reprint Critical exponents for line tension and dipole density difference from lipid monolayer domain boundary fluctuations
    Michael C Heinrich
    Department of Chemistry, Institute for Medicine and Engineering, and Laboratory for Research on the Structure of Matter, University of Pennsylvania, 231 South 34th Street, Philadelphia, PA 19104, USA
    J Phys Chem B 112:8063-8. 2008
    ..Additionally, photobleaching effects and domain size influence were quantified and found to be small in our system. We suggest that the method of flicker spectroscopy can be helpful in identifying line-active compounds...
  61. ncbi request reprint Photon correlation spectroscopy of brain mitochondrial populations: application to traumatic brain injury
    Jonathan Lifshitz
    Traumatic Brain Injury Laboratory, Department of Neurosurgery, University of Pennsylvania, Philadelphia, PA 19104, USA
    Exp Neurol 197:318-29. 2006
    ..PCS provides quantitative indices of refractive index and size in isolated mitochondrial populations, aiding the evaluation of mitochondria in degenerative diseases...
  62. pmc The receptor deformation model of TCR triggering
    Zhengyu Ma
    Department of Pediatrics, The Children s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA
    FASEB J 22:1002-8. 2008
    ..By introducing mechanical force into a model of T cell signal initiation, the receptor deformation model provides potential mechanistic solutions to the sensitivity and specificity of TCR triggering...
  63. ncbi request reprint Anionic poly(amino acid)s dissolve F-actin and DNA bundles, enhance DNase activity, and reduce the viscosity of cystic fibrosis sputum
    Jay X Tang
    Dept of Physics, Brown University, Providence, Rhode Island, USA
    Am J Physiol Lung Cell Mol Physiol 289:L599-605. 2005
    ..These findings suggest that soluble multivalent anions have potential alone or in combination with other mucolytic agents to selectively dissociate the large bundles of charged biopolymers that form in CF sputum...
  64. ncbi request reprint Comparison of filamin A-induced cross-linking and Arp2/3 complex-mediated branching on the mechanics of actin filaments
    Fumihiko Nakamura
    Hematology Division, Brigham and Women s Hospital, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 277:9148-54. 2002
    ..They suggest complementary roles for actin branching and cross-linking in cellular actin mechanics in vivo...
  65. ncbi request reprint Elastic contributions dominate the viscoelastic properties of sputum from cystic fibrosis patients
    Heidi Nielsen
    Department of Chemistry, Roskilde University, DK 4000 Roskilde, Denmark
    Biophys Chem 112:193-200. 2004
    ..Severing of actin filaments lowers the elastic modulus by 30-40%, but maintains viscoelastic integrity, while reduction of thiols in the glycoproteins nearly completely fluidizes the samples...
  66. ncbi request reprint Structural analysis of gelsolin using synchrotron protein footprinting
    Janna G Kiselar
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York, 10461 1602, USA
    Mol Cell Proteomics 2:1120-32. 2003
    ..The results demonstrate that radiolytic protein footprinting can provide detailed structural information on the conformational dynamics of ligand-induced structural changes, and the data provide a detailed model for gelsolin activation...
  67. ncbi request reprint Polyacrylamide hydrogels for cell mechanics: steps toward optimization and alternative uses
    Casey E Kandow
    Department of Biology, Wayne State University, Detroit, Michigan 48202, USA
    Methods Cell Biol 83:29-46. 2007
    ..Our intention is to provide users of this gel system with a number of improved and tested options as this method advances toward optimization...
  68. pmc Metal ion-induced lateral aggregation of filamentous viruses fd and M13
    Jay X Tang
    Physics Department, Indiana University, Bloomington, Indiana 47405, USA
    Biophys J 83:566-81. 2002
    ..The understanding of metal ions-induced reversible aggregation based on solution electrostatics may lead to potential applications in molecular biology and medicine...
  69. pmc Surface-anchored monomeric agonist pMHCs alone trigger TCR with high sensitivity
    Zhengyu Ma
    Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America
    PLoS Biol 6:e43. 2008
    ..Based on these observations, we propose the receptor deformation model of TCR triggering to explain the remarkable sensitivity and specificity of TCR triggering...
  70. ncbi request reprint Role of the cytoskeleton in signaling networks
    Gabor Forgacs
    Department of Physics and Biology, University of Missouri, Columbia, MO 65211, USA
    J Cell Sci 117:2769-75. 2004
    ..The results demonstrate that the network of signaling proteins is intimately linked to the cytoskeleton, suggesting that this interconnected filamentous structure plays a crucial and distinct functional role in signal transduction...
  71. ncbi request reprint Purification of salmon thrombin and its potential as an alternative to mammalian thrombins in fibrin sealants
    Sarah E Michaud
    Hematology Division, Brigham and Women s Hospital, LMRC 302, 221 Longwood Ave, Boston, MA 02115, USA
    Thromb Res 107:245-54. 2002
    ....
  72. pmc Neurite branching on deformable substrates
    Lisa A Flanagan
    Division of Experimental Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Neuroreport 13:2411-5. 2002
    ..These results show that mechanical properties of the substrate specifically direct the formation of neurite branches, which are critical for appropriate synaptic connections during development and regeneration...
  73. ncbi request reprint Lamellar phase of stacked two-dimensional rafts of actin filaments
    Gerard C L Wong
    Materials Department, Physics Department, Biomolecular Science and Engineering Program, University of California, Santa Barbara, California 93106, USA
    Phys Rev Lett 91:018103. 2003
    ..This behavior is generic for alkali earth divalent ions Mg2+, Ca2+, Sr2+, and Ba2+, and the structural transitions are achieved without any architecture-specific actin-binding linker proteins...
  74. pmc Enhanced neurite growth from mammalian neurons in three-dimensional salmon fibrin gels
    Yo El Ju
    Division of Experimental Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Biomaterials 28:2097-108. 2007
    ..These data demonstrate that salmon fibrin encourages the growth of neurites from mammalian neurons and suggest that salmon fibrin may be a beneficial scaffold for neuronal regrowth after CNS injury...

Research Grants26

  1. Dissolution of polyelectrolyte bundles in airway fluids
    Paul Janmey; Fiscal Year: 2005
    ..abstract_text> ..
  2. Effects of phosphoinositidies on cytoskeletal structure
    Paul Janmey; Fiscal Year: 2006
    ..abstract_text> ..
  3. Mechanical control of cell growth and differentiation
    Paul Janmey; Fiscal Year: 2009
    ....
  4. Novel antibacterial agents resistant to inactivation by polyelectrolytes
    Paul A Janmey; Fiscal Year: 2010
    ..The current proposal will create or retain 4 jobs. ..
  5. Mechanical control of cell growth and differentiation
    Paul A Janmey; Fiscal Year: 2010
    ..We propose to determine optimal stiffness for specific cell functions and design soft biocompatible materials to support cell growth and function. ..
  6. REVERSIBLE FORMATION AND STRUCTURE OF ACTIN NETWORKS
    Paul Janmey; Fiscal Year: 2001
    ..A correlation to protein-phosphoinositide interactions in vivo will be made using cell-permeant peptides that selectively block gelsoline-phosphoinositide binding using platelets, neutrophils, and neuronal cells as test systems. ..
  7. MECHANISMS OF NEUROFILAMENT TRANSLOCATION
    Paul Janmey; Fiscal Year: 2004
    ....
  8. Mechanical control of cell growth and differentiation
    Paul Janmey; Fiscal Year: 2009
    ..We propose to determine optimal stiffness for specific cell functions and design soft biocompatible materials to support cell growth and function. ..