Research Topics
| M O JamesSummaryAffiliation: University of Florida Country: USA Publications
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Publications
Slow O-demethylation of methyl triclosan to triclosan, which is rapidly glucuronidated and sulfonated in channel catfish liver and intestineMargaret O James
Department of Medicinal Chemistry, University of Florida, Gainesville, FL 32610 0485, United States
Aquat Toxicol 124:72-82. 2012..These rates are much higher than the rates of demethylation of methyl triclosan, and suggest that triclosan would be rapidly cleared and unlikely to bioaccumulate in catfish tissues...
Prenatal and postnatal expression of glutathione transferase ζ 1 in human liver and the roles of haplotype and subject age in determining activity with dichloroacetateWenjun Li
Department of Medicinal Chemistry, College of Pharmacy, University of Florida, Gainesville, FL 32610 0485, USA
Drug Metab Dispos 40:232-9. 2012..In summary, we report a neonatal onset and an age-related increase in GSTZ1 protein expression during human liver development. Haplotype influenced GSTZ1 activity with DCA but not protein expression...
Intestinal bioavailability and biotransformation of 3-hydroxybenzo(a)pyrene in an isolated perfused preparation from channel catfish, Ictalurus punctatusM O James
Department of Medicinal Chemistry, College of Pharmacy, University of Florida, Gainesville, FL 32610 0485, USA
Drug Metab Dispos 29:721-8. 2001..These studies demonstrate the rapid phase 2 conjugation of a phenolic benzo(a)pyrene metabolite in intestinal mucosa, and the transfer of the phase 2 sulfate and glucuronide conjugates to blood...- Triclosan is a potent inhibitor of estradiol and estrone sulfonation in sheep placentaMargaret O James
Department of Medicinal Chemistry, College of Pharmacy, University of Florida, Gainesville, FL 32610 0485, USA
Environ Int 36:942-9. 2010..The high potency of Triclosan as an inhibitor of estrogen sulfotransferase activity raises concern about its possible effects on the ability of the placenta to supply estrogen to the fetus, and in turn on fetal growth and development... - Glucuronidation and sulfonation, in vitro, of the major endocrine-active metabolites of methoxychlor in the channel catfish, Ictalurus punctatus, and induction following treatment with 3-methylcholanthreneMargaret O James
Department of Medicinal Chemistry, University of Florida, Gainesville, FL 32610 0485, United States
Aquat Toxicol 86:227-38. 2008..This work showed that although OH-MXC and HPTE could be eliminated by glucuronidation and sulfonation, the phase II pathways were less efficient than the phase I pathway leading to formation of these endocrine-active metabolites... - Properties and regional expression of a CYP3A-like protein in channel catfish intestineMargaret O James
Department of Medicinal Chemistry, University of Florida, Gainesville, FL 32610 0485, USA
Aquat Toxicol 72:361-71. 2005..77 pmol/min/mg protein, mean +/- S.D., n = 5 and 1.45 +/- 0.42 in these respective segments. The results of this study strongly suggest that CYP3A is important in the first pass metabolism of dietary xenobiotics in untreated fish... - Increased toxicity of benzo(a)pyrene-7,8-dihydrodiol in the presence of polychlorobiphenylolsMargaret O James
Department of Medicinal Chemistry, College of Pharmacy, University of Florida, P O Box 100485, Gainesville, FL 32610 0485, USA
Mar Environ Res 58:343-6. 2004..These studies suggest a mechanism for synergy of toxicity of PAH and PCBs... - Steroid catabolism in marine and freshwater fishMargaret O James
Department of Medicinal Chemistry, University of Florida, PO Box 100485, 1600 SW Archer Road, Gainesville, FL 32610 0485, United States
J Steroid Biochem Mol Biol 127:167-75. 2011..Recent studies in this area are presented, with particular focus on phase II (conjugative) pathways... - Dietary modulation of phase 1 and phase 2 activities with benzo(a)pyrene and related compounds in the intestine but not the liver of the channel catfish, Ictalurus punctatusM O James
Department of Medicinal Chemistry, College of Pharmacy, University of Florida, Gainesville 32610 0485, USA
Drug Metab Dispos 25:346-54. 1997..Epoxide hydrolase activity with styrene oxide as substrate was not affected by diet in intestinal microsomes... - Clinical pharmacology and toxicology of dichloroacetateP W Stacpoole
Department of Medicine, College of Medicine, University of Florida, Gainesville, USA
Environ Health Perspect 106:989-94. 1998..It remains to be determined whether important differences in its metabolism and toxicology exist in humans between environmentally and clinically relevant doses... - Demethylation of the pesticide methoxychlor in liver and intestine from untreated, methoxychlor-treated, and 3-methylcholanthrene-treated channel catfish (Ictalurus punctatus): evidence for roles of CYP1 and CYP3A family isozymesLeah D Stuchal
Department of Medicinal Chemistry, Health Science Center, University of Florida, Gainesville, FL 32610, USA
Drug Metab Dispos 34:932-8. 2006..The results suggest that the formation of estrogenic metabolites from methoxychlor would be more rapid in catfish coexposed to CYP1 inducers... - Sulfation and glucuronidation of benzo[a]pyrene-7,8-dihydrodiol in intestinal mucosa of channel catfish (Ictalurus punctatus)P Van Den Hurk
Department of Medicinal Chemistry, College of Pharmacy, University of Florida, PO Box 100485, Gainesville, FL 32610, USA
Mar Environ Res 50:11-5. 2000..The slow rates for sulfation and glucuronidation of BaP-7,8-diol may explain its relatively high systemic availability when ingested or produced by intestinal phase 1 enzymes... - Glucuronidation of polychlorinated biphenylols and UDP-glucuronic acid concentrations in channel catfish liver and intestineJames C Sacco
Department of Medicinal Chemistry, University of Florida, PO Box 100485, Gainesville, FL 32610 0485, USA
Drug Metab Dispos 36:623-30. 2008..Although liver has a greater glucuronidation capacity than proximal intestine, the properties of intestinal UGTs in channel catfish enable them to efficiently glucuronidate low concentrations of OH-PCBs... - Influence of dietary Coexposure to benzo(a)pyrene on the biotransformation and distribution of 14C-methoxychlor in the channel catfish (Ictalurus punctatus)Beatrice A Nyagode
Department of Medicinal Chemistry, University of Florida, Gainesville, Florida 32610 0485, USA
Toxicol Sci 108:320-9. 2009.... - Liquid chromatography-tandem mass spectrometry method for the simultaneous determination of delta-ALA, tyrosine and creatinine in biological fluidsNatalia M Felitsyn
Department of Medicine Division of Endocrinology and Metabolism, College of Medicine, University of Florida, Gainesville, FL 32610, USA
Clin Chim Acta 350:219-30. 2004..There is currently no facile, sensitive and specific method to measure these analytes simultaneously... - Sulfonation of 17beta-estradiol and inhibition of sulfotransferase activity by polychlorobiphenylols and celecoxib in channel catfish, Ictalurus punctatusLi Quan Wang
Department of Medicinal Chemistry, College of Pharmacy, University of Florida, Gainesville, FL 32610, USA
Aquat Toxicol 81:286-92. 2007.... - Pharmacologic or genetic ablation of maleylacetoacetate isomerase increases levels of toxic tyrosine catabolites in rodentsChandramohan V Ammini
Division of Endocrinology and Metabolism, Department of Medicine, University of Florida, Gainesville, FL 32610, USA
Biochem Pharmacol 66:2029-38. 2003..These results demonstrate that pharmacologic or genetic ablation of MAAI cause potentially toxic concentrations of tyrosine intermediates to accumulate in mice and perhaps in other species... - Effects of the pesticide methoxychlor on gene expression in the liver and testes of the male largemouth bass (Micropterus salmoides)Jason L Blum
Department of Pharmacology and Therapeutics, University of Florida, Gainesville, FL 32611, USA
Aquat Toxicol 86:459-69. 2008.... - Inhibition and recovery of rat hepatic glutathione S-transferase zeta and alteration of tyrosine metabolism following dichloroacetate exposure and withdrawalXu Guo
Division of Endocrinology and Metabolism, Department of Medicine, University of Florida, FL 32610, USA
Drug Metab Dispos 34:36-42. 2006..The liver/body weight ratio increased in the high-dose group after 8 weeks of DCA. These studies demonstrate that short-term administration of DCA inhibits rat liver GSTz across the wide concentration range to which humans are exposed... - Intestinal and hepatic microsomal metabolism of testosterone and progesterone by a 3 alpha-hydroxysteroid dehydrogenase to the 3 alpha-hydroxy derivatives in the channel catfish, Ictalurus punctatusZhen Lou
Department of Medicinal Chemistry, College of Pharmacy, University of Florida, P.O. Box 100485, Gainesville 32610-0485, USA
J Steroid Biochem Mol Biol 82:413-24. 2002..Thus, 3 alpha-hydroxysteroid dehydrogenase is an important pathway in intestinal microsomes of the channel catfish... - In vitro inhibition of human hepatic and cDNA-expressed sulfotransferase activity with 3-hydroxybenzo[a]pyrene by polychlorobiphenylolsLi-Quan Wang
Department of Medicinal Chemistry, University of Florida, Gainesville, Florida 32610-0485, USA
Environ Health Perspect 113:680-7. 2005..These findings demonstrate an interaction between potentially toxic hydroxylated metabolites of PCBs and polycyclic aromatic hydrocarbons, which could result in reduced clearance by sulfonation... - Unified gas chromatographic-mass spectrometric method for quantitating tyrosine metabolites in urine and plasmaAlbert L Shroads
Division of Endocrinology and Metabolism, Department of Medicine, University of Florida, Gainesville, FL 32610, USA
J Chromatogr B Analyt Technol Biomed Life Sci 808:153-61. 2004..Thus far, the method has been successfully applied in the analysis of over 5000 urine and plasma samples from humans and rodents... - Glucuronidation in the polar bear (Ursus maritimus)James C Sacco
Department of Medicinal Chemistry, University of Florida, P.O. Box 100485, 1600 S.W. Archer Road, Room P6-20, Gainesville, FL 32610-0485, USA
Mar Environ Res 58:475-9. 2004..This study demonstrates that UGT with high activity for 3-OH-BaP and other substrates is present in polar bear liver... - Hydroxylated polychlorinated biphenyls as inhibitors of the sulfation and glucuronidation of 3-hydroxy-benzo[a]pyrenePeter van den Hurk
Department of Medicinal Chemistry, University of Florida, Gainesville, Florida 32610, USA
Environ Health Perspect 110:343-8. 2002..Inhibition of glucuronosyltransferase by low concentrations of OH-PCBs has not been reported before and may have important consequences for the bioavailability, bioaccumulation, and toxicity of other phenolic environmental contaminants... - Pharmacokinetics and metabolism of [14C]dichloroacetate in male Sprague-Dawley rats. Identification of glycine conjugates, including hippurate, as urinary metabolites of dichloroacetateM O James
Departments of Medicinal Chemistry and Pharmacodynamics, College of Pharmacy, University of Florida, Gainesville, FL 32610 0485, USA
Drug Metab Dispos 26:1134-43. 1998.... - Inhibition of sulfotransferases by xenobioticsLi Quan Wang
Department of Medicinal Chemistry, College of Pharmacy, University of Florida, Gainesville, FL 32610, USA
Curr Drug Metab 7:83-104. 2006..This manuscript will review the literature concerning the inhibition of SULTs by xenobiotics including isoform-selective effects, inhibition kinetics and health effects resulting from the inhibition... - Polychlorobiphenylols are selective inhibitors of human phenol sulfotransferase 1A1 with 4-nitrophenol as a substrateLi-Quan Wang
Department of Medicinal Chemistry, College of Pharmacy, University of Florida, Gainesville 32610-0485, USA
Chem Biol Interact 159:235-46. 2006.... - Sulfonation of environmental chemicals and their metabolites in the polar bear (Ursus maritimus)James C Sacco
Department of Medicinal Chemistry, College of Pharmacy, University of Florida, Gainesville, FL 32610, USA
Drug Metab Dispos 33:1341-8. 2005..The hexachlorinated polychlorinated biphenylols, TCPM, and PCP were poor substrates for sulfonation, suggesting that this may be one reason why these substances and structurally similar xenobiotics persist in polar bears... - Triclosan as a substrate and inhibitor of 3'-phosphoadenosine 5'-phosphosulfate-sulfotransferase and UDP-glucuronosyl transferase in human liver fractionsLi-Quan Wang
Department of Medicinal Chemistry, College of Pharmacy, University of Florida, Gainesville FL 32610-0485, USA
Drug Metab Dispos 32:1162-9. 2004..These findings reveal that the commonly used bactericide triclosan is a selective inhibitor of the glucuronidation and sulfonation of phenolic xenobiotics... - Mitochondrion as a novel site of dichloroacetate biotransformation by glutathione transferase zeta 1Wenjun Li
Department of Medicinal Chemistry, College of Pharmacy, University of Florida, Gainesville, FL 32610 0485, USA
J Pharmacol Exp Ther 336:87-94. 2011..We conclude that the mitochondrion is a novel site of DCA biotransformation catalyzed by GSTZ1, an enzyme colocalized in cytosol and mitochondrial matrix... - Stimulation of transactivation of the largemouth bass estrogen receptors alpha, beta-a, and beta-b by methoxychlor and its mono- and bis-demethylated metabolites in HepG2 cellsJason L Blum
Department of Pharmacology and Therapeutics, University of Florida, Gainesville FL 32611, USA
J Steroid Biochem Mol Biol 108:55-63. 2008.... - Sulfotransferase 2A1 forms estradiol-17-sulfate and celecoxib switches the dominant product from estradiol-3-sulfate to estradiol-17-sulfateLi-Quan Wang
Department of Medicinal Chemistry, College of Pharmacy, University of Florida, Gainesville, FL 32610, USA
J Steroid Biochem Mol Biol 96:367-74. 2005..The ability of celecoxib to alter the position of sulfonation of E2 may in part explain its success in the experimental prevention and treatment of breast cancer... - Intestinal bioavailability and biotransformation of 3,3',4,4'-tetrachlorobiphenyl (CB 77) in in situ preparations of channel catfish following dietary induction of CYP1AAdriana M Doi
Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Skip Bertman Dr, Baton Rouge, LA 70803, USA
Aquat Toxicol 77:33-42. 2006..In addition, it is likely that previous [14C]-TCB bioavailability findings in the perfused intestine may be based on TCB concentration gradients rather than biotransformation... - Binding of 3-hydroxybenzo[a]pyrene to bovine hemoglobin and albuminNarumi Sugihara
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Sanzou, Gakuen-cho, Fukuyama, Hiroshima, Japan
J Biochem Mol Toxicol 17:239-47. 2003..These results indicate that an oxidative process is involved in formation of the heme adduct and that 3-OHBaP or BaP-3G might be a precursor of the bound metabolite...