Michael A Jacobs
Affiliation: University of Washington
- Comprehensive transposon mutant library of Pseudomonas aeruginosaMichael A Jacobs
Department of Medicine, University of Washington Genome Center, Box 352145, Seattle, WA 98195 2145, USA
Proc Natl Acad Sci U S A 100:14339-44. 2003..The transposons used to generate the mutant collection have added features that should facilitate downstream studies of gene expression, protein localization, epistasis, and chromosome engineering...
- Comparison of Francisella tularensis genomes reveals evolutionary events associated with the emergence of human pathogenic strainsLaurence Rohmer
Department of Genome Sciences, University of Washington, Campus Box 357710, 1705 NE Pacific Street Seattle, Washington 98195, USA
Genome Biol 8:R102. 2007....
- Chloroplast His-to-Asp signal transduction: a potential mechanism for plastid gene regulation in Heterosigma akashiwo (Raphidophyceae)Melinda R Duplessis
Department of Biology, University of Washington, Seattle, WA 98195 5325, USA
BMC Evol Biol 7:70. 2007..Hara et Chihara [syn. H. carterae (Hulburt) F.J.R. Taylor]. The presence, structure and putative function of this protein pair are discussed in the context of their evolutionary homologues...
- Chloroplast genome sequencing analysis of Heterosigma akashiwo CCMP452 (West Atlantic) and NIES293 (West Pacific) strainsRose Ann Cattolico
Department of Biology, University of Washington, Box 355325, Seattle, WA 98195 5325, USA
BMC Genomics 9:211. 2008..The technique has been used to sequence chloroplast DNA of two Heterosigma akashiwo strains. This raphidophyte has served as a model system for studies of stramenopile chloroplast biogenesis and evolution...
- Rapid 16S rRNA next-generation sequencing of polymicrobial clinical samples for diagnosis of complex bacterial infectionsStephen J Salipante
Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA
PLoS ONE 8:e65226. 2013..The described method is sufficiently rapid (theoretically compatible with same-day turnaround times) and inexpensive for routine clinical use...
- Large-insert genome analysis technology detects structural variation in Pseudomonas aeruginosa clinical strains from cystic fibrosis patientsHillary S Hayden
Genome Center, University of Washington, Seattle, WA 98195, USA
Genomics 91:530-7. 2008..aeruginosa and validates a technology that complements emerging, short-read sequencing methods that are better suited to characterizing single-nucleotide polymorphisms than structural variation...
- How to make a defined near-saturation mutant library. Case 1: Pseudomonas aeruginosa PAO1Michael A Jacobs
Department of Medicine, University of Washington Genome Center, Seattle, WA, USA
Methods Mol Biol 416:133-52. 2008..It is clear that many avenues of research have been accelerated by this collection and that additional large mutant strain collections will further aid in defining gene function and biological processes in pathogens...
- Genomic analysis of the emergence of 20th century epidemic dysenteryLaurence Rohmer
Department of Microbiology, University of Washington, Seattle, WA, USA
BMC Genomics 15:355. 2014..Sd1 is only detected in the context of human infections, and the circumstances under which epidemics emerge and regress remain unknown...
- Multiarm high-throughput integration site detection: limitations of LAM-PCR technology and optimization for clonal analysisMichael A Harkey
Department of Transplantation Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
Stem Cells Dev 16:381-92. 2007....
- Comparison of HIV-derived lentiviral and MLV-based gammaretroviral vector integration sites in primate repopulating cellsBrian C Beard
Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
Mol Ther 15:1356-65. 2007..These data help define the relative risk of insertional mutagenesis with MLV-, HIV-, and simian immunodeficiency virus (SIV)-based vectors in a highly relevant primate model...
- A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogateLarry A Gallagher
Department of Genome Sciences, University of Washington, Campus Box 355065, 1705 NE Pacific Street, Seattle, WA 98195, USA
Proc Natl Acad Sci U S A 104:1009-14. 2007..The library provides a resource for virtually complete identification of genes involved in virulence and other nonessential processes...