Brant Isakson

Summary

Affiliation: University of Virginia
Country: USA

Publications

  1. pmc Localized expression of an Ins(1,4,5)P3 receptor at the myoendothelial junction selectively regulates heterocellular Ca2+ communication
    Brant E Isakson
    Robert M Berne Cardiovascular Research Center, and Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, P O Box 801394, Charlottesville, VA 22908, USA
    J Cell Sci 121:3664-73. 2008
  2. ncbi request reprint Ca2+ and inositol 1,4,5-trisphosphate-mediated signaling across the myoendothelial junction
    Brant E Isakson
    Department of Molecular Physiology and Biological Physics, Robert M Berne Cardiovascular Research Center, University of Virginia School of Medicine, Charlottesville 22908, USA
    Circ Res 100:246-54. 2007
  3. pmc Laminin-332 alters connexin profile, dye coupling and intercellular Ca2+ waves in ciliated tracheal epithelial cells
    Brant E Isakson
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, University of Virginia Charlottesville, Virginia 22908, USA
    Respir Res 7:105. 2006
  4. ncbi request reprint Oxidized phospholipids alter vascular connexin expression, phosphorylation, and heterocellular communication
    Brant E Isakson
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Arterioscler Thromb Vasc Biol 26:2216-21. 2006
  5. pmc Plasminogen activator inhibitor-1 regulates myoendothelial junction formation
    Katherine R Heberlein
    Robert M Berne Cardiovascular Research Center, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Circ Res 106:1092-102. 2010
  6. pmc Site-specific connexin phosphorylation is associated with reduced heterocellular communication between smooth muscle and endothelium
    Adam C Straub
    Robert M Berne Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908, USA
    J Vasc Res 47:277-86. 2010
  7. ncbi request reprint Heterocellular contact at the myoendothelial junction influences gap junction organization
    Brant E Isakson
    Cardiovascular Research Center, University of Virginia School of Medicine, Charlottesville 22908, USA
    Circ Res 97:44-51. 2005
  8. ncbi request reprint Connexin40 and connexin43 in mouse aortic endothelium: evidence for coordinated regulation
    Brant E Isakson
    Department of Molecular Physiology and Biological Physics, Robert M Berne Cardiovascular Research Center, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Am J Physiol Heart Circ Physiol 290:H1199-205. 2006
  9. pmc Incidence of protein on actin bridges between endothelium and smooth muscle in arterioles demonstrates heterogeneous connexin expression and phosphorylation
    Brant E Isakson
    Department of Molecular Physiology and Biological Physics, University of Virginia Health Science System, Charlottesville, Virginia, USA
    Am J Physiol Heart Circ Physiol 294:H2898-904. 2008
  10. pmc Oxidized phospholipid species promote in vivo differential cx43 phosphorylation and vascular smooth muscle cell proliferation
    Scott R Johnstone
    University of Virginia School of Medicine, PO Box 801394 Charlottesville VA 29908, USA
    Am J Pathol 175:916-24. 2009

Collaborators

Detail Information

Publications22

  1. pmc Localized expression of an Ins(1,4,5)P3 receptor at the myoendothelial junction selectively regulates heterocellular Ca2+ communication
    Brant E Isakson
    Robert M Berne Cardiovascular Research Center, and Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, P O Box 801394, Charlottesville, VA 22908, USA
    J Cell Sci 121:3664-73. 2008
    ..This data implicates the MEJ as being a unique cell-signaling domain in the vasculature...
  2. ncbi request reprint Ca2+ and inositol 1,4,5-trisphosphate-mediated signaling across the myoendothelial junction
    Brant E Isakson
    Department of Molecular Physiology and Biological Physics, Robert M Berne Cardiovascular Research Center, University of Virginia School of Medicine, Charlottesville 22908, USA
    Circ Res 100:246-54. 2007
    ..We conclude that 1,4,5-trisphosphate and Ca2+ originating in the VSMCs induces the secondary increase in EC [Ca2+]i but stimulation of the ECs generates a Ca2+ dependent response in the VSMCs...
  3. pmc Laminin-332 alters connexin profile, dye coupling and intercellular Ca2+ waves in ciliated tracheal epithelial cells
    Brant E Isakson
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, University of Virginia Charlottesville, Virginia 22908, USA
    Respir Res 7:105. 2006
    ....
  4. ncbi request reprint Oxidized phospholipids alter vascular connexin expression, phosphorylation, and heterocellular communication
    Brant E Isakson
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Arterioscler Thromb Vasc Biol 26:2216-21. 2006
    ..Lipoprotein-derived phospholipid oxidation products (OxPAPC) play a critical role in atherosclerosis, and we hypothesized that they may act as trigger molecules causing the changes in connexin expression...
  5. pmc Plasminogen activator inhibitor-1 regulates myoendothelial junction formation
    Katherine R Heberlein
    Robert M Berne Cardiovascular Research Center, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Circ Res 106:1092-102. 2010
    ..Plasminogen activator inhibitor-1 (PAI-1) is a biomarker for several vascular disease states; however, its target of action within the vessel wall is undefined...
  6. pmc Site-specific connexin phosphorylation is associated with reduced heterocellular communication between smooth muscle and endothelium
    Adam C Straub
    Robert M Berne Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908, USA
    J Vasc Res 47:277-86. 2010
    ..The functional consequences of phosphorylation state of the connexins (Cx) at the MEJ have not been explored...
  7. ncbi request reprint Heterocellular contact at the myoendothelial junction influences gap junction organization
    Brant E Isakson
    Cardiovascular Research Center, University of Virginia School of Medicine, Charlottesville 22908, USA
    Circ Res 97:44-51. 2005
    ..We speculate that specific gap junction organization may be a vital component of EC-VSMC contact at the MEJ...
  8. ncbi request reprint Connexin40 and connexin43 in mouse aortic endothelium: evidence for coordinated regulation
    Brant E Isakson
    Department of Molecular Physiology and Biological Physics, Robert M Berne Cardiovascular Research Center, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Am J Physiol Heart Circ Physiol 290:H1199-205. 2006
    ....
  9. pmc Incidence of protein on actin bridges between endothelium and smooth muscle in arterioles demonstrates heterogeneous connexin expression and phosphorylation
    Brant E Isakson
    Department of Molecular Physiology and Biological Physics, University of Virginia Health Science System, Charlottesville, Virginia, USA
    Am J Physiol Heart Circ Physiol 294:H2898-904. 2008
    ....
  10. pmc Oxidized phospholipid species promote in vivo differential cx43 phosphorylation and vascular smooth muscle cell proliferation
    Scott R Johnstone
    University of Virginia School of Medicine, PO Box 801394 Charlottesville VA 29908, USA
    Am J Pathol 175:916-24. 2009
    ..Taken together, these results demonstrate that post-translational phosphorylation of Cx43 could be a key factor in the pathogenesis of atherosclerosis...
  11. pmc Biological and biophysical properties of vascular connexin channels
    Scott Johnstone
    Robert M Berne Cardiovascular Research Center, University of Virginia School of Medicine, Charlottesville, Virginia 29908, USA
    Int Rev Cell Mol Biol 278:69-118. 2009
    ....
  12. ncbi request reprint Connexins: gaps in our knowledge of vascular function
    Xavier F Figueroa
    Department of Molecular Physiology and Biological Physics, Cardiovascular Research Center, University of Virginia, Charlottesville, Virginia 22908, USA
    Physiology (Bethesda) 19:277-84. 2004
    ....
  13. pmc Identification of a novel macrophage phenotype that develops in response to atherogenic phospholipids via Nrf2
    Alexandra Kadl
    Department of Pharmacology, University of Virginia, Charlottesville, VA 22908, USA
    Circ Res 107:737-46. 2010
    ..In atherosclerosis, oxidative tissue damage accompanies chronic inflammation; however, macrophage phenotypic changes in response to oxidatively modified molecules are not known...
  14. pmc The myoendothelial junction: breaking through the matrix?
    Katherine R Heberlein
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottsville, Virginia 22908, USA
    Microcirculation 16:307-22. 2009
    ..We will also highlight the need for novel approaches to studying the myoendothelial junction in order to comprehend the cellular biology associated with this structure...
  15. pmc Pannexin1 regulates ╬▒1-adrenergic receptor- mediated vasoconstriction
    Marie Billaud
    Robert M Berne Cardiovascular Research Center, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Circ Res 109:80-5. 2011
    ..Pannexins (Panx) are purine-releasing channels permeable to the vasoconstrictor ATP and thus may play a role in the coordination of vascular smooth muscle cell constriction...
  16. pmc Compartmentalized connexin 43 s-nitrosylation/denitrosylation regulates heterocellular communication in the vessel wall
    Adam C Straub
    Department of Molecular Physiology and Biological Physics, Robert M Berne Cardiovascular Research Center, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Arterioscler Thromb Vasc Biol 31:399-407. 2011
    ..To determine whether S-nitrosylation of connexins (Cxs) modulates gap junction communication between endothelium and smooth muscle...
  17. pmc The importance of serine 161 in the sodium channel beta3 subunit for modulation of Na(V)1.2 gating
    Ellen C Merrick
    Department of Anesthesiology, University of Virginia Health System, Charlottesville, VA, 22908 0710, USA
    Pflugers Arch 460:743-53. 2010
    ..These studies suggest that modification of beta3S161 may be important in modulating Na-channel gating...
  18. pmc Pannexin 1 channels mediate 'find-me' signal release and membrane permeability during apoptosis
    Faraaz B Chekeni
    Beirne B Carter Center for Immunology Research, University of Virginia, Charlottesville, Virginia 22908, USA
    Nature 467:863-7. 2010
    ..Collectively, these data identify PANX1 as a plasma membrane channel mediating the regulated release of find-me signals and selective plasma membrane permeability during apoptosis, and a new mechanism of PANX1 activation by caspases...
  19. ncbi request reprint Vascular gap junctions in hypertension
    Xavier F Figueroa
    Unidad de Regulaci├│n Neurohumoral, Departamento de Ciencias Fisiologicas, Facultad de Ciencias Biologicas, Pontificia Universidad Catolica de Chile, Santiago, Chile
    Hypertension 48:804-11. 2006
  20. ncbi request reprint Cell-cell communication in heterocellular cultures of alveolar epithelial cells
    Brant E Isakson
    Department of Physiology, University of Arizona Health Sciences Center, Tucson, AZ 85724, USA
    Am J Respir Cell Mol Biol 29:552-61. 2003
    ..In conclusion, AT1 and AT2 cells have unique connexin profiles that allow for functional coupling and distinct intercellular pathways for coordination of Ca2+ signaling...
  21. ncbi request reprint Culturing alveolar type I, alveolar type II, and mixed cell populations by altering the extracellular matrix promotes changes in intercellular signaling mechanisms
    Scott Boitano
    Department of Zollogy, University of Wyoming, Laramie, WY 82071 3166, USA
    Chest 121:35S-36S. 2002
  22. ncbi request reprint Extracellular matrix-driven alveolar epithelial cell differentiation in vitro
    Colin O Olsen
    Arizona Respiratory Center, University of Arizona Health Sciences Center, Tucson, AZ 85724 5051, USA
    Exp Lung Res 31:461-82. 2005
    ....

Research Grants4

  1. Vascular Cell Regulation of Connexins
    Brant Isakson; Fiscal Year: 2005
    ..These results will be compared to similar experiments on in situ wild type and connexin KO animals. Combined, these methods will help delineate the physiological role of connexins between EC and SMC. ..
  2. Mechanisms of Heterocellular Signaling at the Myoendothelial Junction
    Brant Isakson; Fiscal Year: 2009
    ..We propose to study the ways in which vascular cells utilize this structure to maintain vascular function. ..
  3. Mechanisms of Heterocellular Signaling at the Myoendothelial Junction
    Brant E Isakson; Fiscal Year: 2010
    ..We propose to study the ways in which vascular cells utilize this structure to maintain vascular function. ..