James Imlay

Summary

Affiliation: University of Illinois
Country: USA

Publications

  1. ncbi request reprint How oxygen damages microbes: oxygen tolerance and obligate anaerobiosis
    James A Imlay
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Adv Microb Physiol 46:111-53. 2002
  2. ncbi request reprint Iron-sulphur clusters and the problem with oxygen
    James A Imlay
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Mol Microbiol 59:1073-82. 2006
  3. pmc Cellular defenses against superoxide and hydrogen peroxide
    James A Imlay
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Annu Rev Biochem 77:755-76. 2008
  4. ncbi request reprint Pathways of oxidative damage
    James A Imlay
    Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA
    Annu Rev Microbiol 57:395-418. 2003
  5. ncbi request reprint What biological purpose is served by superoxide reductase?
    James A Imlay
    Department of Microbiology, University of Illinois, Urbana 61801, USA
    J Biol Inorg Chem 7:659-63. 2002
  6. pmc How obligatory is anaerobiosis?
    James A Imlay
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Mol Microbiol 68:801-4. 2008
  7. pmc Substantial DNA damage from submicromolar intracellular hydrogen peroxide detected in Hpx- mutants of Escherichia coli
    Sunny Park
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 102:9317-22. 2005
  8. pmc Manganese import is a key element of the OxyR response to hydrogen peroxide in Escherichia coli
    Adil Anjem
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Mol Microbiol 72:844-58. 2009
  9. ncbi request reprint Repair of oxidized iron-sulfur clusters in Escherichia coli
    Ouliana Djaman
    Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA
    J Biol Chem 279:44590-9. 2004
  10. pmc Submicromolar hydrogen peroxide disrupts the ability of Fur protein to control free-iron levels in Escherichia coli
    Shery Varghese
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Mol Microbiol 64:822-30. 2007

Collaborators

  • Christopher Rensing
  • R I Mackie
  • FRANKLIN OUTTEN
  • DANIEL HASSETT
  • Gang Liu
  • G C Ferreira
  • JOAN VALENTINE
  • Lee Macomber
  • Sunny Park
  • Shery Varghese
  • Anh N Woodmansee
  • Sergei Korshunov
  • Radha Krishnakumar
  • Kevin R Messner
  • Surabhi Mishra
  • Pinggui Wu
  • Adil Anjem
  • Soojin Jang
  • William Munroe
  • Oleksandr Gakh
  • James M Slauch
  • Lauren Costa Seaver
  • Ouliana Djaman
  • Alexandra H Smith
  • Christopher D Pericone
  • Sergei S Korshunov
  • Jian Ku Shang
  • Elizabeth A Mollo
  • Armando Durazo
  • Byoungkwan Kim
  • Karin R C Imlay
  • Chandra Srinivasan
  • Carolyn Kingsley
  • Edith Butler Gralla
  • Amy Wu
  • Sungjo Park
  • Grazia Isaya
  • Xiaojun You
  • Maureen Craig
  • Jeffrey N Weiser
  • Yue Tang

Detail Information

Publications33

  1. ncbi request reprint How oxygen damages microbes: oxygen tolerance and obligate anaerobiosis
    James A Imlay
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Adv Microb Physiol 46:111-53. 2002
    ..Their failure to divest themselves of enzymes that can be directly damaged by molecular oxygen suggests that evolution has not yet provided economical options to them...
  2. ncbi request reprint Iron-sulphur clusters and the problem with oxygen
    James A Imlay
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Mol Microbiol 59:1073-82. 2006
    ..Thus, the history of Fe-S clusters is an unusual one that has profoundly shaped contemporary microbial ecology...
  3. pmc Cellular defenses against superoxide and hydrogen peroxide
    James A Imlay
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Annu Rev Biochem 77:755-76. 2008
    ..Current research seeks to identify these, and bacteria comprise an exceptionally accessible experimental system that has provided many of the answers. This manuscript reviews recent developments and identifies remaining puzzles...
  4. ncbi request reprint Pathways of oxidative damage
    James A Imlay
    Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA
    Annu Rev Microbiol 57:395-418. 2003
    ..The vulnerability of cells to oxidation lies at the root of obligate anaerobiosis, spontaneous mutagenesis, and the use of oxidative stress as a biological weapon...
  5. ncbi request reprint What biological purpose is served by superoxide reductase?
    James A Imlay
    Department of Microbiology, University of Illinois, Urbana 61801, USA
    J Biol Inorg Chem 7:659-63. 2002
    ..Some potential benefits of superoxide reduction have been proposed; these and several others are considered here...
  6. pmc How obligatory is anaerobiosis?
    James A Imlay
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Mol Microbiol 68:801-4. 2008
    ..Evidently, oxidative defences in anaerobes can be robust. In all likelihood, they are critical for the movement of bacteria through aerobic environments to new anaerobic habitats...
  7. pmc Substantial DNA damage from submicromolar intracellular hydrogen peroxide detected in Hpx- mutants of Escherichia coli
    Sunny Park
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 102:9317-22. 2005
    ..The rate constant of the Fenton reaction measured at physiological pH was much higher than under the acidic conditions that were used to determine the commonly cited value...
  8. pmc Manganese import is a key element of the OxyR response to hydrogen peroxide in Escherichia coli
    Adil Anjem
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Mol Microbiol 72:844-58. 2009
    ..Accordingly, during H2O2 stress, mutants that cannot import manganese and/or are unable to sequester iron suffer high rates of protein oxidation...
  9. ncbi request reprint Repair of oxidized iron-sulfur clusters in Escherichia coli
    Ouliana Djaman
    Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA
    J Biol Chem 279:44590-9. 2004
    ....
  10. pmc Submicromolar hydrogen peroxide disrupts the ability of Fur protein to control free-iron levels in Escherichia coli
    Shery Varghese
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Mol Microbiol 64:822-30. 2007
    ..The induction of Fur synthesis by the OxyR response comprises one evolutionary solution to that problem...
  11. pmc Contrasting sensitivities of Escherichia coli aconitases A and B to oxidation and iron depletion
    Shery Varghese
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    J Bacteriol 185:221-30. 2003
    ..coli synthesizes a transport system. Thus, AcnB may be retained as the primary aconitase because the lability of its exposed cluster allows E. coli to sense and respond to iron depletion...
  12. pmc High levels of intracellular cysteine promote oxidative DNA damage by driving the fenton reaction
    Sunny Park
    Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA
    J Bacteriol 185:1942-50. 2003
    ..More generally, this study provides a new example of a situation in which the vulnerability of cells to oxidative DNA damage is strongly affected by their physiological state...
  13. ncbi request reprint Micromolar intracellular hydrogen peroxide disrupts metabolism by damaging iron-sulfur enzymes
    Soojin Jang
    Department of Microbiology, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    J Biol Chem 282:929-37. 2007
    ..The extreme vulnerability of these enzymes may explain why many organisms, including mammals, deploy H2O2 to suppress microbial growth...
  14. pmc Increasing the oxidative stress response allows Escherichia coli to overcome inhibitory effects of condensed tannins
    Alexandra H Smith
    Department of Animal Sciences, University of Illinois, Urbana, Illinois 61801, USA
    Appl Environ Microbiol 69:3406-11. 2003
    ..Our results indicate that wattle condensed tannins are toxic to E. coli in aerobic medium primarily because they generate H(2)O(2). The oxidative stress response helps E. coli strains to overcome their inhibitory effect...
  15. ncbi request reprint Are respiratory enzymes the primary sources of intracellular hydrogen peroxide?
    Lauren Costa Seaver
    Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA
    J Biol Chem 279:48742-50. 2004
    ..That source has not yet been identified. In respiring cells the rate of H2O2 production was approximately 0.5% the rate of total oxygen consumption, with only modest changes when cells used different carbon sources...
  16. ncbi request reprint A mechanism by which nitric oxide accelerates the rate of oxidative DNA damage in Escherichia coli
    Anh N Woodmansee
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Mol Microbiol 49:11-22. 2003
    ..Further, mutants that lack flavin reductase were resistant to NO/H2O2, and overproducing strains were hypersensitive. We discuss the possibility that H2O2 and NO synergize when macrophages attack captive bacteria...
  17. pmc Intracellular copper does not catalyze the formation of oxidative DNA damage in Escherichia coli
    Lee Macomber
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    J Bacteriol 189:1616-26. 2007
    ..These observations do not explain how copper suppresses iron-mediated damage. However, it is clear that copper does not catalyze significant oxidative DNA damage in vivo; therefore, copper toxicity must occur by a different mechanism...
  18. pmc The iron-sulfur clusters of dehydratases are primary intracellular targets of copper toxicity
    Lee Macomber
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 106:8344-9. 2009
    ..Copper efflux by dedicated export systems, chelation by glutathione, and cluster repair by assembly systems all enhance the resistance of cells to this metal...
  19. ncbi request reprint Mechanism of superoxide and hydrogen peroxide formation by fumarate reductase, succinate dehydrogenase, and aspartate oxidase
    Kevin R Messner
    Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA
    J Biol Chem 277:42563-71. 2002
    ..In contrast, succinate dehydrogenase, with high potential clusters, generates O2*- exclusively. The identities of enzyme autoxidation products are significant because O2*- and H2O2 damage cells in different ways...
  20. pmc Why do bacteria use so many enzymes to scavenge hydrogen peroxide?
    Surabhi Mishra
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Arch Biochem Biophys 525:145-60. 2012
    ..It is hoped that the resolution of these issues will lead to an understanding of stress resistance that is more accurate and perceptive...
  21. pmc Two sources of endogenous hydrogen peroxide in Escherichia coli
    Sergei Korshunov
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Mol Microbiol 75:1389-401. 2010
    ..These two examples reveal biochemical and physiological arrangements through which evolution has minimized the rate of intracellular oxidant formation...
  22. ncbi request reprint In vitro quantitation of biological superoxide and hydrogen peroxide generation
    Kevin R Messner
    Department of Microbiology, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Methods Enzymol 349:354-61. 2002
  23. ncbi request reprint Reduced flavins promote oxidative DNA damage in non-respiring Escherichia coli by delivering electrons to intracellular free iron
    Anh N Woodmansee
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    J Biol Chem 277:34055-66. 2002
    ..coli when respiration is blocked. It remains unclear whether flavins or other reductants drive Fenton chemistry in respiring cells...
  24. ncbi request reprint A potential role for periplasmic superoxide dismutase in blocking the penetration of external superoxide into the cytosol of Gram-negative bacteria
    Sergei S Korshunov
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Mol Microbiol 43:95-106. 2002
    ..One role of periplasmic SOD may be to avert this damage. In contrast, periplasmic SOD was ineffective at lowering the extracellular super-oxide concentration and, therefore, may have little impact upon HOONO formation...
  25. ncbi request reprint Quantitation of intracellular free iron by electron paramagnetic resonance spectroscopy
    Anh N Woodmansee
    Department of Microbiology, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Methods Enzymol 349:3-9. 2002
  26. pmc Differences in enzymatic properties allow SodCI but not SodCII to contribute to virulence in Salmonella enterica serovar Typhimurium strain 14028
    Radha Krishnakumar
    Department of Microbiology, University of Illinois, Urbana, 61801, USA
    J Bacteriol 186:5230-8. 2004
    ..We propose that this novel property of SodCI is crucial to its ability to contribute to virulence in serovar Typhimurium...
  27. pmc Detection and quantification of superoxide formed within the periplasm of Escherichia coli
    Sergei Korshunov
    Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA
    J Bacteriol 188:6326-34. 2006
    ..coli. This endogenous superoxide may create oxidative stress in that compartment and be a primary substrate of CuZnSOD...
  28. pmc Structural properties of periplasmic SodCI that correlate with virulence in Salmonella enterica serovar Typhimurium
    Radha Krishnakumar
    Department of Microbiology, University of Illinois, B103 Chemical and Life Sciences Laboratory, 601 S Goodwin Ave, Urbana, IL 61801, USA
    J Bacteriol 189:4343-52. 2007
    ..We propose a model to account for the various properties of SodCI and how they contribute to bacterial survival in the phagosome...
  29. pmc Mechanism of Escherichia coli inactivation on palladium-modified nitrogen-doped titanium dioxide
    Pinggui Wu
    Department of Materials Science and Engineering, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Biomaterials 31:7526-33. 2010
    ....
  30. ncbi request reprint Bactericidal antibiotics and oxidative stress: a radical proposal
    Daniel J Hassett
    Department of Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45221, USA
    ACS Chem Biol 2:708-10. 2007
    ..This proposal represents an abrupt departure from conventional models, and it will attract further experimental tests...
  31. ncbi request reprint Mitochondrial iron detoxification is a primary function of frataxin that limits oxidative damage and preserves cell longevity
    Oleksandr Gakh
    Department of Pediatric, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Hum Mol Genet 15:467-79. 2006
    ....
  32. pmc Factors contributing to hydrogen peroxide resistance in Streptococcus pneumoniae include pyruvate oxidase (SpxB) and avoidance of the toxic effects of the fenton reaction
    Christopher D Pericone
    Departments of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Bacteriol 185:6815-25. 2003
    ..pneumoniae has a novel mechanism to avoid the toxic effects of the Fenton reaction...
  33. pmc Only one of a wide assortment of manganese-containing SOD mimicking compounds rescues the slow aerobic growth phenotypes of both Escherichia coli and Saccharomyces cerevisiae strains lacking superoxide dismutase enzymes
    William Munroe
    Department of Chemistry and Biochemistry, UCLA, Los Angeles, CA 90095 1569, USA
    J Inorg Biochem 101:1875-82. 2007
    ..Our results suggest the possibility that the beneficial effects of some of the so-called "SOD mimic drugs" may be due to some property other than in vivo superoxide dismutase activity...

Research Grants18

  1. The Physiology of Oxidative Stress in Escherichia coli
    James Imlay; Fiscal Year: 2006
    ..coli will provide a blueprint for efforts to solve key issues in oxidative stress: obligate anaerobiosis, the killing mechanism of) hagocytes, and endogenous oxidative stress in higher organisms. ..
  2. MECHANISM OF OXIDATIVE DNA DAMAGE IN MODEL ORGANISMS
    James Imlay; Fiscal Year: 2002
    ..Further, by understanding how metabolic perturbations affect DNA oxidation, we may be able to anticipate the affects of drug therapies upon mutagenesis and cell death. ..
  3. IMPACT OF SUPEROXIDE ON THE PHYSIOLOGY OF MODEL BACTERIA
    James Imlay; Fiscal Year: 2002
    ..Other, such as glucose-6-phosphate dehydrogenases, may be understandable only if some of the toxicity of these drugs arises from NADPH depletion rather than from reactive oxygen species. ..