Research Topics
Genomes and Genes | Suzanne T IldstadSummaryAffiliation: University of Louisville Country: USA Publications
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Publications
Preconditioning of NOD mice with anti-CD8 mAb and costimulatory blockade enhances chimerism and tolerance and prevents diabetes, while depletion of alpha beta-TCR+ and CD4+ cells negates the effectSuzanne T Ildstad
Institute for Cellular Therapeutics, Department of Pathology, University of Louisville, 570 South Preston St, Suite 404, Louisville, KY 40202 1760, USA
Blood 105:2577-84. 2005..Notably, disease prevention and reversal of autoimmunity was absolutely correlated with the establishment of chimerism. These studies have important implications for the design of novel clinical approaches to treat type 1 diabetes...- Flt3-ligand-mobilized peripheral blood, but not Flt3-ligand-expanded bone marrow, facilitating cells promote establishment of chimerism and toleranceYiming Huang
Institute for Cellular Therapeutics, University of Louisville, 570 S. Preston Street, Suite 404, Louisville, Kentucky 40202-1760, USA
Stem Cells 24:936-48. 2006..These findings may have implications in development of strategies for retaining function of ex vivo manipulated FCs and HSCs... 
Strategic nonmyeloablative conditioning: CD154:CD40 costimulatory blockade at primary bone marrow transplantation promotes engraftment for secondary bone marrow transplantation after engraftment failureHong Xu
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202, USA
J Immunol 181:6616-24. 2008..The prevention of generation of anti-donor Abs at primary BMT is critical for successful secondary BMT...- Dissociation between peripheral blood chimerism and tolerance to hindlimb composite tissue transplants: preferential localization of chimerism in donor boneDina N Rahhal
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
Transplantation 88:773-81. 2009..Mixed chimerism induces donor-specific tolerance to composite tissue allotransplants (CTAs). In the present studies, we used a nonmyeloablative conditioning approach to establish chimerism and promote CTA acceptance... - Plasmacytoid precursor dendritic cells from NOD mice exhibit impaired function: are they a component of diabetes pathogenesis?Yiming Huang
Institute for Cellular Therapeutics, University of Louisville, Louisville, Kentucky, USA
Diabetes 57:2360-70. 2008..Here, we evaluated the phenotype and function of CD8(+)/TCR(-) FCs from NOD mice... 
Innate and adaptive immune responses are tolerized in chimeras prepared with nonmyeloablative conditioningHong Xu
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
Transplantation 93:469-76. 2012..Mixed chimerism is an effective approach for tolerance induction in transplantation. Strategies to achieve mixed chimerism with relatively low toxicity have significantly expanded the clinical use of chimerism...- Evidence that FoxP3+ regulatory T cells may play a role in promoting long-term acceptance of composite tissue allotransplantsLarry D Bozulic
Regenerex, LLC, Louisville, KY, USA
Transplantation 91:908-15. 2011..In this study, we evaluated the role of Treg in peripheral tolerance to composite tissue allografts (CTA)... - Bone marrow transplantation temporarily improves pancreatic function in streptozotocin-induced diabetes: potential involvement of very small embryonic-like cellsYiming Huang
Institute for Cellular Therapeutics, James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA
Transplantation 89:677-85. 2010..The role of bone marrow (BM)-derived cells in pancreatic beta-cell regeneration remains unresolved. We examined whether BM-derived cells are recruited to the site of moderate pancreatic injury and contribute to beta-cell regeneration... - NK cells play a critical role in the regulation of class I-deficient hemopoietic stem cell engraftment: evidence for NK tolerance correlates with receptor editingYiming Huang
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202, USA
J Immunol 175:3753-61. 2005..Moreover, our data show that bone marrow-derived elements significantly contribute to NK cell development and tolerance... - Addition of cyclophosphamide to T-cell depletion-based nonmyeloablative conditioning allows donor T-cell engraftment and clonal deletion of alloreactive host T-cells after bone marrow transplantationHong Xu
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
Transplantation 83:954-63. 2007..In the present studies, we demonstrate that nonmyeloablative conditioning and BM cell infusion modulate innate and adaptive host immune responses... - Plasmacytoid precursor dendritic cells facilitate allogeneic hematopoietic stem cell engraftmentIsabelle J Fugier-Vivier
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202, USA
J Exp Med 201:373-83. 2005..These results are the first to define a direct functional role for p-preDCs in HSC engraftment, and also suggest that p-preDCs need to be in a certain state of maturation/activation to be fully functional... - A delay in bone marrow transplantation after partial conditioning improves engraftmentHong Xu
Institute for Cellular Therapeutics, University of Louisville, Kentucky 40202, USA
Transplantation 77:819-26. 2004.... - Flt3-ligand treatment prevents diabetes in NOD micePaula M Chilton
Institute for Cellular Therapeutics, University of Louisville, Louisville, Kentucky 40202 1760, USA
Diabetes 53:1995-2002. 2004..Therefore, Flt3-ligand treatment and/or the establishment of mixed chimerism in prediabetic candidates may provide a benign and novel approach to treat diabetes... - Costimulatory blockade of CD154-CD40 in combination with T-cell lymphodepletion results in prevention of allogeneic sensitizationHong Xu
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
Blood 111:3266-75. 2008..These findings may be clinically relevant to prevent allosensitization with minimal toxicity and point to humoral immunity as playing a dominant role in alloreactivity in sensitized recipients... - CD45 congenic bone marrow transplantation: evidence for T cell-mediated immunityHong Xu
Institute for Cellular Therapeutics, University of Louisville, 570 South Preston Street, Louisville, Kentucky 40202-1760, USA
Stem Cells 22:1039-48. 2004.... - Endogenous bone marrow derived cells express retinal pigment epithelium cell markers and migrate to focal areas of RPE damageYang Li
Department of Ophthalmology and Visual Sciences, University of Louisville, Louisville, Kentucky, USA
Invest Ophthalmol Vis Sci 48:4321-7. 2007.... - Humoral immunity is the dominant barrier for allogeneic bone marrow engraftment in sensitized recipientsHong Xu
Institute for Cellular Therapeutics, Ste 404, University of Louisville, 570 S Preston St, Louisville, KY 40202-1760, USA
Blood 108:3611-9. 2006..These findings have important implications for the management of sensitized transplant recipients and for xenotransplantation in which B-cell reactivity is a predominant barrier... - TNF-alpha is critical to facilitate hemopoietic stem cell engraftment and functionFrancine Rezzoug
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
J Immunol 180:49-57. 2008..These data demonstrate a critical role for TNF-alpha in mediating FC function. FC may have a significant impact upon the safe use of chimerism to establish tolerance to transplanted organs and tissue... - Production of donor T cells is critical for induction of donor-specific tolerance and maintenance of chimerismHong Xu
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202, USA
J Immunol 172:1463-71. 2004..In addition, donor T cell production directly correlates with the deletion of potentially alloreactive cells... - A clinically feasible approach to induce delayed tolerance in recipients of prior kidney or vascularized composite allotransplantsBo Chen
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY, USA
Transplantation 94:671-8. 2012..Here, we developed a delayed tolerance induction strategy with mixed chimerism through BMT in prior kidney or VCA recipients... - Plasmacytoid dendritic cells regulate autoreactive B cell activation via soluble factors and in a cell-to-cell contact mannerChuanlin Ding
Tumor Immunobiology Program, James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
J Immunol 183:7140-9. 2009..These data suggest the dynamic interplay between pDCs and B cells is required for full activation of autoreactive B cells upon TLR or BCR stimulation... - Differential outcomes in prediabetic vs. overtly diabetic NOD mice nonmyeloablatively conditioned with costimulatory blockadeLarry D Bozulic
Regenerex, LLC, Louisville, KY, USA
Exp Hematol 39:977-85. 2011..Here, we investigated whether multimodal costimulatory blockade would enhance chimerism and promote islet allograft tolerance in spontaneously diabetic nonobese diabetic (NOD) mice... - Hematopoietic stem cells from NOD mice exhibit autonomous behavior and a competitive advantage in allogeneic recipientsPaula M Chilton
Institute for Cellular Therapeutics, University of Louisville, 570 South Preston St, Suite 404, Louisville, KY 40202-1760, USA
Blood 105:2189-97. 2005..These findings may have important implications to understand the evolution of autoimmune disease and impact on potential strategies for cure... - CD8α+ plasmacytoid precursor DCs induce antigen-specific regulatory T cells that enhance HSC engraftment in vivoYiming Huang
Institute for Cellular Therapeutics, University of Louisville, 570 S Preston St, Louisville, KY 40202, USA
Blood 117:2494-505. 2011..These data suggest that FCs induce the production of antigen-specific Tregs in vivo, which potently enhance engraftment of allogeneic HSCs. FCs hold clinical potential because of their ability to remain tolerogenic in vivo... - Fms-related tyrosine kinase 3 expression discriminates hematopoietic stem cells subpopulations with differing engraftment-potential: identifying the most potent combinationYiming Huang
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
Transplantation 85:1175-84. 2008..FL significantly mobilized c-Kit(hi) and c-Kit(lo) Sca-1(+)Lin(-) cells into peripheral blood (PB). Here, we evaluated the influence of Flt3 expression on long-term repopulating ability of HSC subpopulations... - Postinfarct cytokine therapy regenerates cardiac tissue and improves left ventricular functionBuddhadeb Dawn
Institute of Molecular Cardiology, University of Louisville, Louisville, KY 40292, USA
Circ Res 98:1098-105. 2006.... - The beneficial effects of postinfarct cytokine combination therapy are sustained during long-term follow-upSantosh K Sanganalmath
Institute of Molecular Cardiology, University of Louisville, Division of Cardiovascular Medicine, 550 S Jackson St, ACB, 3rd Floor, Louisville, KY 40292, USA
J Mol Cell Cardiol 47:528-35. 2009..To our knowledge, this is the first long-term study of cytokines in postinfarction LV remodeling. The results reveal heretofore unknown differential actions of cytokines and have important translational implications... - Graft facilitating cells are derived from hematopoietic stem cells and functionally require CD3, but are distinct from T lymphocytesH Leighton Grimes
Institute for Cellular Therapeutics University of Louisville, Louisville, KY 40202, USA
Exp Hematol 32:946-54. 2004..The unique function(s) of FC make them an attractive focus for new cell-based therapeutic approaches to enhance HSC engraftment while reducing toxicity, especially when limiting numbers of HSC are available... - A modified model of hindlimb osteomyocutaneous flap for the study of tolerance to composite tissue allograftsLarry A Adamson
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
Microsurgery 27:630-6. 2007..When compared with the classic hind limb transplantation model, these results demonstrate that our heterotopic hind limb flap is less morbid and as an effective experimental model for the study of CTA tolerance... - Cells expressing early cardiac markers reside in the bone marrow and are mobilized into the peripheral blood after myocardial infarctionMagda Kucia
Stem Cell Biology Program, University of Louisville, Louisville, Ken 40202, USA
Circ Res 95:1191-9. 2004..The present findings provide a novel paradigm that could reconcile current controversies and a rationale for investigating the use of BM-derived cardiac progenitors for myocardial regeneration... - Identification of inducible nitric oxide synthase in peripheral blood cells as a mediator of myocardial ischemia/reperfusion injuryYiru Guo
Institute of Molecular Cardiology, University of Louisville, 550 S Jackson Street ACB Bldg, 3rd Floor, Louisville, KY 40202, USA
Basic Res Cardiol 107:253. 2012..The results support heretofore unknown differential actions of iNOS depending on cell source and have important translational implications... - Science of composite tissue allotransplantationBruce Swearingen
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
Transplantation 86:627-35. 2008..This review focuses on the science of CTA, provides a snapshot of where we are in the clinic, and discusses prospects for the future to make the procedures even more widely available... - Sensitized recipients exhibit accelerated but not hyperacute rejection of vascularized composite tissue allograftsShengli Wu
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
Transplantation 92:627-33. 2011..Sensitization is considered a contraindication to VCTA. However, the role of sensitization in VCTA rejection is largely unstudied... - Systemically transferred hematopoietic stem cells home to the subretinal space and express RPE-65 in a mouse model of retinal pigment epithelium damagePelin Atmaca-Sonmez
Department of Ophthalmology and Visual Sciences, University of Louisville, 301 E. Muhammad Ali Blvd, Louisville, KY 40202, USA
Exp Eye Res 83:1295-302. 2006..Furthermore, the RPE-specific marker RPE-65 was expressed on adoptively transferred HSC in the denuded areas... - Matching at the MHC class I K locus is essential for long-term engraftment of purified hematopoietic stem cells: a role for host NK cells in regulating HSC engraftmentYiming Huang
Institute for Cellular Therapeutics and the Department of Surgery, University of Louisville, Louisville, KY, USA
Blood 104:873-80. 2004..Therefore, MHC class I may play a critical role in the initiating events that dictate HSC engraftment and/or NK-mediated rejection following allogeneic transplantation... - Hematopoietic stem cells from the marrow of mice treated with Flt3 ligand are significantly expanded but exhibit reduced engraftment potentialYvonne M Mueller
Institute for Cellular Therapeutics, University of Louisville, Louisville, Kentucky, 40202-1760, USA
Transplantation 73:1177-85. 2002..The reduced syngeneic engraftment of HSC indicates that FL treatment induces intrinsic changes in HSC, resulting in failure of long-term engraftment or self-renewal despite no change in characteristic phenotype of HSC... - Stem cell-based therapeutic applications in retinal degenerative diseasesYiming Huang
Institute for Cellular Therapeutics, University of Louisville, 570 S Preston Street, Suite 404, Louisville, KY 40202 1760, USA
Stem Cell Rev 7:434-45. 2011..In this review, we will focus on stem cell-based therapies in the pipeline and summarize progress in treatment of retinal degenerative disease... - Novel regulatory therapies for prevention of Graft-versus-host diseaseJoseph Leventhal
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY, USA
BMC Med 10:48. 2012.... - Composite tissue allotransplantation in chimeric hosts: part I. Prevention of graft-versus-host diseaseVijay S Gorantla
Division of Plastic and Reconstructive Surgery, Plastic Surgery Research, University of Louisville, 511 South Floyd Street, Louisville, KY 40292, USA
Transplantation 75:922-32. 2003.... - Stem cell-based strategies for the treatment of type 1 diabetes mellitusYujie Wen
University of Louisville, Institute for Cellular Therapeutics, Louisville, KY 40202 1760, USA
Expert Opin Biol Ther 11:41-53. 2011..Stem cell-based strategies to address the problems of shortage in β cells, autoimmune and alloimmune responses have become an area of intense study... - Facilitating cells as a venue to establish mixed chimerism and toleranceEric G Jacquet
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202-1760, USA
Pediatr Transplant 7:348-57. 2003..This invited review focuses on recent developments in stem cell chimerism and tolerance that could bring the use of this approach to induce tolerance to solid organ transplantation one step closer to reality... - CD8(+), alphabeta-TCR(+), and gammadelta-TCR(+) cells in the recipient hematopoietic environment mediate resistance to engraftment of allogeneic donor bone marrowHong Xu
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202, USA
J Immunol 168:1636-43. 2002..Targeting of specific recipient cellular populations may permit conditioning approaches to allow mixed chimerism with minimal morbidity and could potentially avoid the requirement for myelotoxic agents altogether... - Tolerance induction in HLA disparate living donor kidney transplantation by donor stem cell infusion: durable chimerism predicts outcomeJoseph Leventhal
Comprehensive Transplant Center, Northwestern Memorial Hospital, Chicago, IL, USA
Transplantation 95:169-76. 2013..Here we provide intermediate-term follow-up on this phase II trial... - Past, present, and future prospects for inducing donor-specific transplantation tolerance for composite tissue allotransplantationLarry D Bozulic
Institute for Cellular Therapeutics, Louisville, Kentucky
Semin Plast Surg 21:213-25. 2007..This article reviews the history of CTA, current challenges and complications, and offers future directions for CTA research in strategies to induce tolerance... - Transplantation of the hand, face, and composite structures: evolution and current statusGordon R Tobin
Division of Plastic Surgery, Department of Surgery, University of Louisville, Louisville, KY 40292, USA
Clin Plast Surg 34:271-8, ix-x. 2007..All failures reported have been caused by lapses in these parameters. This early experience shows a great potential for application of these new procedures to the most challenging reconstructive needs... - Facilitating cells: novel promoters of stem cell alloengraftment and donor-specific transplantation tolerance in the absence of GVHDYolonda L Colson
Division of Thoracic Surgery, Department of Surgery, Brigham and Women s Hospital, Boston, MA 02115, USA
Crit Rev Oncol Hematol 61:26-43. 2007.... - Mixed xenogeneic chimerism induces donor-specific humoral and cellular immune tolerance for cardiac xenograftsYolonda L Colson
Department of Surgery, Division of Thoracic Surgery, Brigham and Women s Hospital, Boston, MA 02115, USA
J Immunol 173:5827-34. 2004.... - Baboon bone-marrow xenotransplant in a patient with advanced HIV disease: case report and 8-year follow-upMarian G Michaels
University of Pittsburgh, School of Medicine, Pittsburgh, PA, USA
Transplantation 78:1582-9. 2004..Long-term improvement was not achieved; however, no adverse events occurred, and no evidence of transmission of xenogeneic infections was found...