Suzanne T Ildstad

Summary

Affiliation: University of Louisville
Country: USA

Publications

  1. pmc Dissociation between peripheral blood chimerism and tolerance to hindlimb composite tissue transplants: preferential localization of chimerism in donor bone
    Dina N Rahhal
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
    Transplantation 88:773-81. 2009
  2. ncbi Flt3-ligand-mobilized peripheral blood, but not Flt3-ligand-expanded bone marrow, facilitating cells promote establishment of chimerism and tolerance
    Yiming Huang
    Institute for Cellular Therapeutics, University of Louisville, 570 S Preston Street, Suite 404, Louisville, Kentucky 40202 1760, USA
    Stem Cells 24:936-48. 2006
  3. pmc Strategic nonmyeloablative conditioning: CD154:CD40 costimulatory blockade at primary bone marrow transplantation promotes engraftment for secondary bone marrow transplantation after engraftment failure
    Hong Xu
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202, USA
    J Immunol 181:6616-24. 2008
  4. pmc Plasmacytoid precursor dendritic cells from NOD mice exhibit impaired function: are they a component of diabetes pathogenesis?
    Yiming Huang
    Institute for Cellular Therapeutics, University of Louisville, Louisville, Kentucky, USA
    Diabetes 57:2360-70. 2008
  5. pmc Bone marrow transplantation temporarily improves pancreatic function in streptozotocin-induced diabetes: potential involvement of very small embryonic-like cells
    Yiming Huang
    Institute for Cellular Therapeutics, James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA
    Transplantation 89:677-85. 2010
  6. pmc Innate and adaptive immune responses are tolerized in chimeras prepared with nonmyeloablative conditioning
    Hong Xu
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
    Transplantation 93:469-76. 2012
  7. ncbi NK cells play a critical role in the regulation of class I-deficient hemopoietic stem cell engraftment: evidence for NK tolerance correlates with receptor editing
    Yiming Huang
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202, USA
    J Immunol 175:3753-61. 2005
  8. ncbi Addition of cyclophosphamide to T-cell depletion-based nonmyeloablative conditioning allows donor T-cell engraftment and clonal deletion of alloreactive host T-cells after bone marrow transplantation
    Hong Xu
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
    Transplantation 83:954-63. 2007
  9. pmc Evidence that FoxP3+ regulatory T cells may play a role in promoting long-term acceptance of composite tissue allotransplants
    Larry D Bozulic
    Regenerex, LLC, Louisville, KY, USA
    Transplantation 91:908-15. 2011
  10. pmc Simultaneous bone marrow and composite tissue transplantation in rats treated with nonmyeloablative conditioning promotes tolerance
    Hong Xu
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
    Transplantation 95:301-8. 2013

Collaborators

Detail Information

Publications47

  1. pmc Dissociation between peripheral blood chimerism and tolerance to hindlimb composite tissue transplants: preferential localization of chimerism in donor bone
    Dina N Rahhal
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
    Transplantation 88:773-81. 2009
    ..Mixed chimerism induces donor-specific tolerance to composite tissue allotransplants (CTAs). In the present studies, we used a nonmyeloablative conditioning approach to establish chimerism and promote CTA acceptance...
  2. ncbi Flt3-ligand-mobilized peripheral blood, but not Flt3-ligand-expanded bone marrow, facilitating cells promote establishment of chimerism and tolerance
    Yiming Huang
    Institute for Cellular Therapeutics, University of Louisville, 570 S Preston Street, Suite 404, Louisville, Kentucky 40202 1760, USA
    Stem Cells 24:936-48. 2006
    ..These findings may have implications in development of strategies for retaining function of ex vivo manipulated FCs and HSCs...
  3. pmc Strategic nonmyeloablative conditioning: CD154:CD40 costimulatory blockade at primary bone marrow transplantation promotes engraftment for secondary bone marrow transplantation after engraftment failure
    Hong Xu
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202, USA
    J Immunol 181:6616-24. 2008
    ..The prevention of generation of anti-donor Abs at primary BMT is critical for successful secondary BMT...
  4. pmc Plasmacytoid precursor dendritic cells from NOD mice exhibit impaired function: are they a component of diabetes pathogenesis?
    Yiming Huang
    Institute for Cellular Therapeutics, University of Louisville, Louisville, Kentucky, USA
    Diabetes 57:2360-70. 2008
    ..Here, we evaluated the phenotype and function of CD8(+)/TCR(-) FCs from NOD mice...
  5. pmc Bone marrow transplantation temporarily improves pancreatic function in streptozotocin-induced diabetes: potential involvement of very small embryonic-like cells
    Yiming Huang
    Institute for Cellular Therapeutics, James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA
    Transplantation 89:677-85. 2010
    ..The role of bone marrow (BM)-derived cells in pancreatic beta-cell regeneration remains unresolved. We examined whether BM-derived cells are recruited to the site of moderate pancreatic injury and contribute to beta-cell regeneration...
  6. pmc Innate and adaptive immune responses are tolerized in chimeras prepared with nonmyeloablative conditioning
    Hong Xu
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
    Transplantation 93:469-76. 2012
    ..Mixed chimerism is an effective approach for tolerance induction in transplantation. Strategies to achieve mixed chimerism with relatively low toxicity have significantly expanded the clinical use of chimerism...
  7. ncbi NK cells play a critical role in the regulation of class I-deficient hemopoietic stem cell engraftment: evidence for NK tolerance correlates with receptor editing
    Yiming Huang
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202, USA
    J Immunol 175:3753-61. 2005
    ..Moreover, our data show that bone marrow-derived elements significantly contribute to NK cell development and tolerance...
  8. ncbi Addition of cyclophosphamide to T-cell depletion-based nonmyeloablative conditioning allows donor T-cell engraftment and clonal deletion of alloreactive host T-cells after bone marrow transplantation
    Hong Xu
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
    Transplantation 83:954-63. 2007
    ..In the present studies, we demonstrate that nonmyeloablative conditioning and BM cell infusion modulate innate and adaptive host immune responses...
  9. pmc Evidence that FoxP3+ regulatory T cells may play a role in promoting long-term acceptance of composite tissue allotransplants
    Larry D Bozulic
    Regenerex, LLC, Louisville, KY, USA
    Transplantation 91:908-15. 2011
    ..In this study, we evaluated the role of Treg in peripheral tolerance to composite tissue allografts (CTA)...
  10. pmc Simultaneous bone marrow and composite tissue transplantation in rats treated with nonmyeloablative conditioning promotes tolerance
    Hong Xu
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
    Transplantation 95:301-8. 2013
    ..Delayed VCA is not clinically applicable due to the time constraints associated with procurement from deceased donors. A more clinically relevant approach to perform both BMT and VCA simultaneously was evaluated...
  11. ncbi A delay in bone marrow transplantation after partial conditioning improves engraftment
    Hong Xu
    Institute for Cellular Therapeutics, University of Louisville, Kentucky 40202, USA
    Transplantation 77:819-26. 2004
    ..In the present study we examined the effect of the timing of marrow infusion on engraftment in nonmyeloablatively conditioned mice...
  12. pmc Plasmacytoid precursor dendritic cells facilitate allogeneic hematopoietic stem cell engraftment
    Isabelle J Fugier-Vivier
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202, USA
    J Exp Med 201:373-83. 2005
    ..These results are the first to define a direct functional role for p-preDCs in HSC engraftment, and also suggest that p-preDCs need to be in a certain state of maturation/activation to be fully functional...
  13. ncbi CD45 congenic bone marrow transplantation: evidence for T cell-mediated immunity
    Hong Xu
    Institute for Cellular Therapeutics, University of Louisville, 570 South Preston Street, Louisville, Kentucky 40202 1760, USA
    Stem Cells 22:1039-48. 2004
    ....
  14. pmc Costimulatory blockade of CD154-CD40 in combination with T-cell lymphodepletion results in prevention of allogeneic sensitization
    Hong Xu
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
    Blood 111:3266-75. 2008
    ..These findings may be clinically relevant to prevent allosensitization with minimal toxicity and point to humoral immunity as playing a dominant role in alloreactivity in sensitized recipients...
  15. ncbi A modified model of hindlimb osteomyocutaneous flap for the study of tolerance to composite tissue allografts
    Larry A Adamson
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
    Microsurgery 27:630-6. 2007
    ..When compared with the classic hind limb transplantation model, these results demonstrate that our heterotopic hind limb flap is less morbid and as an effective experimental model for the study of CTA tolerance...
  16. ncbi Endogenous bone marrow derived cells express retinal pigment epithelium cell markers and migrate to focal areas of RPE damage
    Yang Li
    Department of Ophthalmology and Visual Sciences, University of Louisville, Louisville, Kentucky, USA
    Invest Ophthalmol Vis Sci 48:4321-7. 2007
    ....
  17. ncbi TNF-alpha is critical to facilitate hemopoietic stem cell engraftment and function
    Francine Rezzoug
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
    J Immunol 180:49-57. 2008
    ..These data demonstrate a critical role for TNF-alpha in mediating FC function. FC may have a significant impact upon the safe use of chimerism to establish tolerance to transplanted organs and tissue...
  18. pmc Humoral immunity is the dominant barrier for allogeneic bone marrow engraftment in sensitized recipients
    Hong Xu
    Institute for Cellular Therapeutics, Ste 404, University of Louisville, 570 S Preston St, Louisville, KY 40202 1760, USA
    Blood 108:3611-9. 2006
    ..These findings have important implications for the management of sensitized transplant recipients and for xenotransplantation in which B-cell reactivity is a predominant barrier...
  19. pmc The beneficial effects of postinfarct cytokine combination therapy are sustained during long-term follow-up
    Santosh K Sanganalmath
    Institute of Molecular Cardiology, University of Louisville, Division of Cardiovascular Medicine, 550 S Jackson St, ACB, 3rd Floor, Louisville, KY 40292, USA
    J Mol Cell Cardiol 47:528-35. 2009
    ..To our knowledge, this is the first long-term study of cytokines in postinfarction LV remodeling. The results reveal heretofore unknown differential actions of cytokines and have important translational implications...
  20. doi Fms-related tyrosine kinase 3 expression discriminates hematopoietic stem cells subpopulations with differing engraftment-potential: identifying the most potent combination
    Yiming Huang
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
    Transplantation 85:1175-84. 2008
    ..FL significantly mobilized c-Kit(hi) and c-Kit(lo) Sca-1(+)Lin(-) cells into peripheral blood (PB). Here, we evaluated the influence of Flt3 expression on long-term repopulating ability of HSC subpopulations...
  21. doi A clinically feasible approach to induce delayed tolerance in recipients of prior kidney or vascularized composite allotransplants
    Bo Chen
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY, USA
    Transplantation 94:671-8. 2012
    ..Here, we developed a delayed tolerance induction strategy with mixed chimerism through BMT in prior kidney or VCA recipients...
  22. pmc Plasmacytoid dendritic cells regulate autoreactive B cell activation via soluble factors and in a cell-to-cell contact manner
    Chuanlin Ding
    Tumor Immunobiology Program, James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    J Immunol 183:7140-9. 2009
    ..These data suggest the dynamic interplay between pDCs and B cells is required for full activation of autoreactive B cells upon TLR or BCR stimulation...
  23. ncbi Flt3-ligand treatment prevents diabetes in NOD mice
    Paula M Chilton
    Institute for Cellular Therapeutics, University of Louisville, Louisville, Kentucky 40202 1760, USA
    Diabetes 53:1995-2002. 2004
    ..Therefore, Flt3-ligand treatment and/or the establishment of mixed chimerism in prediabetic candidates may provide a benign and novel approach to treat diabetes...
  24. pmc Differential outcomes in prediabetic vs. overtly diabetic NOD mice nonmyeloablatively conditioned with costimulatory blockade
    Larry D Bozulic
    Regenerex, LLC, Louisville, KY, USA
    Exp Hematol 39:977-85. 2011
    ..Here, we investigated whether multimodal costimulatory blockade would enhance chimerism and promote islet allograft tolerance in spontaneously diabetic nonobese diabetic (NOD) mice...
  25. ncbi Production of donor T cells is critical for induction of donor-specific tolerance and maintenance of chimerism
    Hong Xu
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202, USA
    J Immunol 172:1463-71. 2004
    ..In addition, donor T cell production directly correlates with the deletion of potentially alloreactive cells...
  26. ncbi Hematopoietic stem cells from NOD mice exhibit autonomous behavior and a competitive advantage in allogeneic recipients
    Paula M Chilton
    Institute for Cellular Therapeutics, University of Louisville, 570 South Preston St, Suite 404, Louisville, KY 40202 1760, USA
    Blood 105:2189-97. 2005
    ..These findings may have important implications to understand the evolution of autoimmune disease and impact on potential strategies for cure...
  27. pmc CD8α+ plasmacytoid precursor DCs induce antigen-specific regulatory T cells that enhance HSC engraftment in vivo
    Yiming Huang
    Institute for Cellular Therapeutics, University of Louisville, 570 S Preston St, Louisville, KY 40202, USA
    Blood 117:2494-505. 2011
    ..These data suggest that FCs induce the production of antigen-specific Tregs in vivo, which potently enhance engraftment of allogeneic HSCs. FCs hold clinical potential because of their ability to remain tolerogenic in vivo...
  28. ncbi Preconditioning of NOD mice with anti-CD8 mAb and costimulatory blockade enhances chimerism and tolerance and prevents diabetes, while depletion of alpha beta-TCR+ and CD4+ cells negates the effect
    Suzanne T Ildstad
    Institute for Cellular Therapeutics, Department of Pathology, University of Louisville, 570 South Preston St, Suite 404, Louisville, KY 40202 1760, USA
    Blood 105:2577-84. 2005
    ..Notably, disease prevention and reversal of autoimmunity was absolutely correlated with the establishment of chimerism. These studies have important implications for the design of novel clinical approaches to treat type 1 diabetes...
  29. ncbi Graft facilitating cells are derived from hematopoietic stem cells and functionally require CD3, but are distinct from T lymphocytes
    H Leighton Grimes
    Institute for Cellular Therapeutics University of Louisville, Louisville, KY 40202, USA
    Exp Hematol 32:946-54. 2004
    ..Whether facilitating cells (FC) develop from T cells or represent a distinct lineage has not been determined...
  30. pmc Cells expressing early cardiac markers reside in the bone marrow and are mobilized into the peripheral blood after myocardial infarction
    Magda Kucia
    Stem Cell Biology Program, University of Louisville, Louisville, Ken 40202, USA
    Circ Res 95:1191-9. 2004
    ..The present findings provide a novel paradigm that could reconcile current controversies and a rationale for investigating the use of BM-derived cardiac progenitors for myocardial regeneration...
  31. pmc Science of composite tissue allotransplantation
    Bruce Swearingen
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
    Transplantation 86:627-35. 2008
    ..This review focuses on the science of CTA, provides a snapshot of where we are in the clinic, and discusses prospects for the future to make the procedures even more widely available...
  32. pmc Postinfarct cytokine therapy regenerates cardiac tissue and improves left ventricular function
    Buddhadeb Dawn
    Institute of Molecular Cardiology, University of Louisville, Louisville, KY 40292, USA
    Circ Res 98:1098-105. 2006
    ....
  33. pmc Identification of inducible nitric oxide synthase in peripheral blood cells as a mediator of myocardial ischemia/reperfusion injury
    Yiru Guo
    Institute of Molecular Cardiology, University of Louisville, 550 S Jackson Street ACB Bldg, 3rd Floor, Louisville, KY 40202, USA
    Basic Res Cardiol 107:253. 2012
    ..The results support heretofore unknown differential actions of iNOS depending on cell source and have important translational implications...
  34. pmc A Critical Role for the TLR4/TRIF Pathway in Allogeneic Hematopoietic Cell Rejection by Innate Immune Cells
    Hong Xu
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY, USA
    Cell Transplant 22:2367-80. 2013
    ..These findings may have direct clinical impact on strategies for conditioning recipients for stem cell transplantation. ..
  35. ncbi Hematopoietic stem cells from the marrow of mice treated with Flt3 ligand are significantly expanded but exhibit reduced engraftment potential
    Yvonne M Mueller
    Institute for Cellular Therapeutics, University of Louisville, Louisville, Kentucky, 40202 1760, USA
    Transplantation 73:1177-85. 2002
    ..The engraftment potential of FL-expanded HSC in the bone marrow compartment has not been evaluated. In this study, we investigated the effect of in vivo administration of FL on the engraftment potential of HSC expanded in the marrow...
  36. pmc Sensitized recipients exhibit accelerated but not hyperacute rejection of vascularized composite tissue allografts
    Shengli Wu
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
    Transplantation 92:627-33. 2011
    ..Sensitization is considered a contraindication to VCTA. However, the role of sensitization in VCTA rejection is largely unstudied...
  37. ncbi Composite tissue allotransplantation in chimeric hosts: part I. Prevention of graft-versus-host disease
    Vijay S Gorantla
    Division of Plastic and Reconstructive Surgery, Plastic Surgery Research, University of Louisville, 511 South Floyd Street, Louisville, KY 40292, USA
    Transplantation 75:922-32. 2003
    ..The aim of this study was to develop an approach to study and prevent GVHD in a mixed chimeric-rat hind-limb transplantation model...
  38. ncbi Systemically transferred hematopoietic stem cells home to the subretinal space and express RPE-65 in a mouse model of retinal pigment epithelium damage
    Pelin Atmaca-Sonmez
    Department of Ophthalmology and Visual Sciences, University of Louisville, 301 E Muhammad Ali Blvd, Louisville, KY 40202, USA
    Exp Eye Res 83:1295-302. 2006
    ..Furthermore, the RPE-specific marker RPE-65 was expressed on adoptively transferred HSC in the denuded areas...
  39. pmc Stem cell-based therapeutic applications in retinal degenerative diseases
    Yiming Huang
    Institute for Cellular Therapeutics, University of Louisville, 570 S Preston Street, Suite 404, Louisville, KY 40202 1760, USA
    Stem Cell Rev 7:434-45. 2011
    ..In this review, we will focus on stem cell-based therapies in the pipeline and summarize progress in treatment of retinal degenerative disease...
  40. ncbi Matching at the MHC class I K locus is essential for long-term engraftment of purified hematopoietic stem cells: a role for host NK cells in regulating HSC engraftment
    Yiming Huang
    Institute for Cellular Therapeutics and the Department of Surgery, University of Louisville, Louisville, KY, USA
    Blood 104:873-80. 2004
    ..Therefore, MHC class I may play a critical role in the initiating events that dictate HSC engraftment and/or NK-mediated rejection following allogeneic transplantation...
  41. ncbi CD8(+), alphabeta-TCR(+), and gammadelta-TCR(+) cells in the recipient hematopoietic environment mediate resistance to engraftment of allogeneic donor bone marrow
    Hong Xu
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202, USA
    J Immunol 168:1636-43. 2002
    ..Targeting of specific recipient cellular populations may permit conditioning approaches to allow mixed chimerism with minimal morbidity and could potentially avoid the requirement for myelotoxic agents altogether...
  42. pmc Novel regulatory therapies for prevention of Graft-versus-host disease
    Joseph Leventhal
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY, USA
    BMC Med 10:48. 2012
    ....
  43. ncbi Facilitating cells as a venue to establish mixed chimerism and tolerance
    Eric G Jacquet
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
    Pediatr Transplant 7:348-57. 2003
    ..This invited review focuses on recent developments in stem cell chimerism and tolerance that could bring the use of this approach to induce tolerance to solid organ transplantation one step closer to reality...
  44. pmc Stem cell-based strategies for the treatment of type 1 diabetes mellitus
    Yujie Wen
    University of Louisville, Institute for Cellular Therapeutics, Louisville, KY 40202 1760, USA
    Expert Opin Biol Ther 11:41-53. 2011
    ..Stem cell-based strategies to address the problems of shortage in β cells, autoimmune and alloimmune responses have become an area of intense study...
  45. pmc Tolerance induction in HLA disparate living donor kidney transplantation by donor stem cell infusion: durable chimerism predicts outcome
    Joseph Leventhal
    Comprehensive Transplant Center, Northwestern Memorial Hospital, Chicago, IL, USA
    Transplantation 95:169-76. 2013
    ..Here we provide intermediate-term follow-up on this phase II trial...
  46. pmc Past, present, and future prospects for inducing donor-specific transplantation tolerance for composite tissue allotransplantation
    Larry D Bozulic
    Institute for Cellular Therapeutics, Louisville, Kentucky
    Semin Plast Surg 21:213-25. 2007
    ..This article reviews the history of CTA, current challenges and complications, and offers future directions for CTA research in strategies to induce tolerance...
  47. ncbi Transplantation of the hand, face, and composite structures: evolution and current status
    Gordon R Tobin
    Division of Plastic Surgery, Department of Surgery, University of Louisville, Louisville, KY 40292, USA
    Clin Plast Surg 34:271-8, ix-x. 2007
    ..All failures reported have been caused by lapses in these parameters. This early experience shows a great potential for application of these new procedures to the most challenging reconstructive needs...