Research Topics
Genomes and Genes
| Mitsuo IkebeSummaryAffiliation: University of Massachusetts Medical School Country: USA Publications
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Publications
The tip of the coiled-coil rod determines the filament formation of smooth muscle and nonmuscle myosinM Ikebe
Departments of Physiology and Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Biol Chem 276:30293-300. 2001..We conclude that the C-terminal end of the coiled-coil domain, but not the nonhelical tail piece, of myosin II is critical for myosin filament formation both in vitro and in vivo...- Conformational change and regulation of myosin moleculesMitsuo Ikebe
Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
Adv Exp Med Biol 565:61-72; discussion 72, 359-69. 2005 - Motor function of unconventional myosinMitsuo Ikebe
Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655-0127, USA
Adv Exp Med Biol 538:143-56; discussion 157. 2003 - A unique ATP hydrolysis mechanism of single-headed processive myosin, myosin IXTaketoshi Kambara
Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Biol Chem 281:4949-57. 2006..After ATP hydrolysis, Pi is quickly released to form a strong actin binding form, and a power stroke takes place... - p116Rip decreases myosin II phosphorylation by activating myosin light chain phosphatase and by inactivating RhoAYasuhiko Koga
Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Biol Chem 280:4983-91. 2005..Based upon these findings, we propose that p116Rip is an important regulatory component that controls the RhoA signaling pathway, thus regulating MLCP activity and myosin phosphorylation in cells... - F-actin and myosin II binding domains in supervillinYu Chen
Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
J Biol Chem 278:46094-106. 2003..Taken together, these results suggest that supervillin may mediate actin and myosin II filament organization at cholesterol-rich membrane domains... - Two functional heads are required for full activation of smooth muscle myosinXiang-Dong Li
Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Biol Chem 278:29435-41. 2003..The present study supports an idea that the two heads of smooth muscle myosin II interact with each other and the presence of two active heads is required for full activation... - Human myosin III is a motor having an extremely high affinity for actinTaketoshi Kambara
Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Biol Chem 281:37291-301. 2006..The present results suggest that myosin IIIA can spend a majority of its ATP hydrolysis cycling time on actin... - Translocation of telokin by cGMP signaling in smooth muscle cellsSatoshi Komatsu
Department of Physiology, University of Massachusetts Medical School, Worcester 01655, USA
Am J Physiol Cell Physiol 283:C752-61. 2002..These results suggest that telokin changes its intracellular localization because of phosphorylation at Ser13 and/or Ser19 via the cGMP-signaling pathway... - Myosin Va becomes a low duty ratio motor in the inhibited formOsamu Sato
Department of Physiology, University of Massachusetts Medical School, Worcester, Massachueetts 01655, USA
J Biol Chem 282:13228-39. 2007.... - Ca2+-induced activation of ATPase activity of myosin Va is accompanied with a large conformational changeXiang-Dong Li
Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
Biochem Biophys Res Commun 315:538-45. 2004..This conformational transition is closely correlated to the actin-activated ATPase activity. These results suggest that the tail and neck domain play a role in the Ca(2+) dependent regulation of myosin Va... - M20, the small subunit of PP1M, binds to microtubulesNorio Takizawa
Department of Physiology and Biomedical Imaging Group, University of Massachusetts Medical School, Worcester 01655, USA
Am J Physiol Cell Physiol 284:C250-62. 2003..These results suggest that M20 has a microtubule binding activity and plays a role in regulating microtubule dynamics... 
Human myosin Vc is a low duty ratio nonprocessive motorShinya Watanabe
Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Biol Chem 283:10581-92. 2008..Our findings suggest that myosin Vc fulfills its function as a cargo transporter by different mechanisms from other myosin V isoforms...
Effect of phosphorylation in the motor domain of human myosin IIIA on its ATP hydrolysis cycleShigeru Komaba
Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655 0127, USA
Biochemistry 49:3695-702. 2010....- A novel regulatory mechanism of myosin light chain phosphorylation via binding of 14-3-3 to myosin phosphataseYasuhiko Koga
Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
Mol Biol Cell 19:1062-71. 2008..The present study revealed a new RhoA/Rho-kinase-dependent regulatory mechanism of myosin II phosphorylation by 14-3-3 that dissociates MLCP from myosin II and attenuates MLCP activity... - Drosophila myosin VIIA is a high duty ratio motor with a unique kinetic mechanismShinya Watanabe
Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
J Biol Chem 281:7151-60. 2006..9. Therefore, myosin VIIA is classified to be a high duty ratio motor. The present results suggested that myosin VIIA can be a processive motor to serve cargo trafficking in cells once it forms a dimer structure... - Supervillin slows cell spreading by facilitating myosin II activation at the cell peripheryNorio Takizawa
Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA
J Cell Sci 120:3792-803. 2007..Thus, the membrane protein SV apparently contributes to myosin II assembly during cell spreading by modulating myosin II regulation by L-MLCK... - The globular tail domain puts on the brake to stop the ATPase cycle of myosin VaXiang Dong Li
Departments of Physiology and Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
Proc Natl Acad Sci U S A 105:1140-5. 2008..We propose that binding of the GTD to the motor domain prevents the movement of the converter/lever arm during ATP hydrolysis cycle, thus inhibiting the chemical cycle of the motor domain... - Phosphorylation of CPI17 and myosin binding subunit of type 1 protein phosphatase by p21-activated kinaseNorio Takizawa
Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
Biochem Biophys Res Commun 297:773-8. 2002..On the other hand, the phosphorylation of MBS by PAK also decreased the MLCP activity. These results raise the possibility that the PAK pathway plays a role in MLCP regulation... - Head-head and head-tail interaction: a general mechanism for switching off myosin II activity in cellsHyun Suk Jung
Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
Mol Biol Cell 19:3234-42. 2008..These interactions are broken when myosin is activated... - Myosin X transports Mena/VASP to the tip of filopodiaHiroshi Tokuo
Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
Biochem Biophys Res Commun 319:214-20. 2004..These results show that M10 is a specific motor carrying Mena/VASP from the root to the tip of the filopodia where extension of actin filament takes place... - Crosstalk between adenosine A1 and β1-adrenergic receptors regulates translocation of PKCε in isolated rat cardiomyocytesSatoshi Komatsu
Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Cell Physiol 227:3201-7. 2012..In that this translocation has been previously associated with the antiadrenergic property of A(1)R, it is apparent that the interactive effects of adenosine and β(1)-adrenergic agonists on function are complex in the heart... - Phospholipid-dependent regulation of the motor activity of myosin XNobuhisa Umeki
Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, Massachusetts, USA
Nat Struct Mol Biol 18:783-8. 2011.... - The tail binds to the head-neck domain, inhibiting ATPase activity of myosin VIIANobuhisa Umeki
Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
Proc Natl Acad Sci U S A 106:8483-8. 2009..We concluded that unconventional myosin that may be present a monomer in the cell can be regulated by intramolecular interaction of the tail with the head... - Cargo binding activates myosin VIIA motor function in cellsTsuyoshi Sakai
Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, MA 01655, USA
Proc Natl Acad Sci U S A 108:7028-33. 2011..Present findings support that MyRip, a cargo molecule, functions as an activator of myosin VIIA transporter function... - The motor activity of myosin-X promotes actin fiber convergence at the cell periphery to initiate filopodia formationHiroshi Tokuo
Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
J Cell Biol 179:229-38. 2007..We conclude that the motor function of the two-headed form of myoX is critical for actin reorganization at the leading edge, leading to filopodia formation... - Impacts of Usher syndrome type IB mutations on human myosin VIIa motor functionShinya Watanabe
Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
Biochemistry 47:9505-13. 2008..Taken together, the results suggest that the mutations responsible for USH1B cause the complete loss of the actin-activated ATPase activity or the reduction of duty ratio of myosin VIIa... - BIG1 is a binding partner of myosin IXb and regulates its Rho-GTPase activating protein activityNobutaka Saeki
Department of Physiology, University of Massachusetts Medical School, 55 Lake Ave, Worcester, Massachusetts 01655, USA
J Biol Chem 280:10128-34. 2005..The findings raise a concept that the myosin transports the signaling molecule as a cargo that functions as a regulator for the myosin molecule... - Myosin IXb is a single-headed minus-end-directed processive motorAkira Inoue
Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655 0127, USA
Nat Cell Biol 4:302-6. 2002..The processive movement of the single-headed myosin IXb cannot be explained by a 'hand-over-hand' mechanism. This suggests that an alternative mechanism must be operating for the processive movement of single-headed myosin IXb... - Activation of myosin Va function by melanophilin, a specific docking partner of myosin VaXiang Dong Li
Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Biol Chem 280:17815-22. 2005..The present finding raises the idea that myosin motors are switched upon their binding to the cargo molecules, thus avoiding the waste of ATP consumption... - Human deafness mutation of myosin VI (C442Y) accelerates the ADP dissociation rateOsamu Sato
Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0127, USA
J Biol Chem 279:28844-54. 2004..The actin gliding velocity of the C442Y mutant decreases significantly at low surface density of myosin VI, suggesting that the mutation hampers the processive movement of myosin VI... - Novel ZIP kinase isoform lacks leucine zipperNorio Takamoto
Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
Arch Biochem Biophys 456:194-203. 2006..These results suggest that the newly found hZIPK-S as well as the long isoform play an important role in the regulation of myosin phosphorylation... - Agonist-induced changes in the phosphorylation of the myosin- binding subunit of myosin light chain phosphatase and CPI17, two regulatory factors of myosin light chain phosphatase, in smooth muscleNaohisa Niiro
Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
Biochem J 369:117-28. 2003.... - Myosin X is a high duty ratio motorKazuaki Homma
Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
J Biol Chem 280:29381-91. 2005..The duty ratio calculated from our kinetic model, 0.6, was consistent with the duty ratio, 0.7, obtained from comparison of K(m ATPase) and K(m motility). Our results suggest that myosin X is a high duty ratio motor... - Determination of human myosin III as a motor protein having a protein kinase activityShigeru Komaba
Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655 0127, USA
J Biol Chem 278:21352-60. 2003..The present study is the first report that clearly demonstrates that the class III myosin is an actin-based motor protein having a protein kinase activity... - The globular tail domain of myosin Va functions as an inhibitor of the myosin Va motorXiang Dong Li
Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Biol Chem 281:21789-98. 2006.... - Dynamics of RhoA and ROKalpha translocation in single living cellsKoji Miyazaki
Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
Cell Biochem Biophys 45:243-54. 2006.... - Adenoprotection of the heart involves phospholipase C-induced activation and translocation of PKC-epsilon to RACK2 in adult rat and mouseRichard A Fenton
Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
Am J Physiol Heart Circ Physiol 297:H718-25. 2009..In conclusion, A(1)R stimulation of the heart is associated with PLC-initiated PKC-epsilon translocation and association with RACK2... - Mechanoenzymatic characterization of human myosin VbShinya Watanabe
Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655-0127, USA
Biochemistry 45:2729-38. 2006..22 +/- 0.03 microm/s) remained constant at a low motor density. The actin filament landing assay revealed that a single HuM5B molecule is sufficient to move the actin filament continuously, indicating that HuM5b is a processive motor... - ZIP kinase is responsible for the phosphorylation of myosin II and necessary for cell motility in mammalian fibroblastsSatoshi Komatsu
Dept. of Physiology, University of Massachusetts Medical School, 55 Lake Ave. North, Worcester, MA 01655, USA
J Cell Biol 165:243-54. 2004..These results suggest that ZIP kinase is critical for myosin phosphorylation and necessary for cell motile processes in mammalian fibroblasts... - cGMP-dependent relaxation of smooth muscle is coupled with the change in the phosphorylation of myosin phosphataseKensei Nakamura
University of Massachusetts Medical School, Department of Physiology, 55 Lake Avenue North, Worcester, MA 01655, USA
Circ Res 101:712-22. 2007..This results in the decrease in MLC phosphorylation and smooth muscle relaxation... - DOC-2/DAB2 is the binding partner of myosin VIAkira Inoue
Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655 0127, USA
Biochem Biophys Res Commun 292:300-7. 2002..The present findings suggest that myosin VI plays a role in transporting DOC-2/DAB2, a Ras cascade signaling molecule, thus involved in Ras signaling pathways... - Protein kinase Cepsilon and the antiadrenergic action of adenosine in rat ventricular myocytesKoji Miyazaki
Dept. of Physiology, S4-242, University of Massachusetts Medical School, 55 Lake Avenue N, Worcester, MA 01655, USA
Am J Physiol Heart Circ Physiol 287:H1721-9. 2004..These results suggest that the translocation of PKCepsilon to the t-tubular system plays an important role in A(1)R-mediated antiadrenergic actions in the heart... - Characterization of the motor activity of mammalian myosin VIIAAkira Inoue
Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0127, USA
J Biol Chem 278:5478-87. 2003..The directionality of myosin VIIA was determined by using the polarity-marked dual fluorescence-labeled actin filaments. It was found that myosin VIIA is a plus-directed motor... - The phosphorylation of myosin II at the Ser1 and Ser2 is critical for normal platelet-derived growth factor induced reorganization of myosin filamentsSatoshi Komatsu
Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
Mol Biol Cell 18:5081-90. 2007.... - Regulation of the function of mammalian myosin and its conformational changeMitsuo Ikebe
Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
Biochem Biophys Res Commun 369:157-64. 2008..These findings suggest the presence of a common scenario for the regulation of motor protein functions... - Dephosphorylation of the two regulatory components of myosin phosphatase, MBS and CPI17Norio Takizawa
Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
FEBS Lett 515:127-32. 2002..On the other hand, phosphorylated CPI17 is readily dephosphorylated thus likely to play a role in regulating fast phosphorylation-dephosphorylation cycle in cells... - Human airway contraction and formoterol-induced relaxation is determined by Ca2+ oscillations and Ca2+ sensitivityAnna Rebekka Ressmeyer
Department of Physiology, University of Massachusetts Medical School, Worcester, 01655, USA
Am J Respir Cell Mol Biol 43:179-91. 2010..Formoterol induces long-lasting relaxation by initially reducing the Ca(2+) sensitivity and, subsequently, the frequency of the Ca(2+) oscillations of the airway SMCs... - The cellular and molecular basis of bitter tastant-induced bronchodilationCheng Hai Zhang
Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America
PLoS Biol 11:e1001501. 2013..We propose that the large decrease in [Ca(2+)]i caused by effective tastant bronchodilators provides an efficient cell-based screening method for identifying potent dilators from among the many thousands of available bitter tastants... - Rho-dependent agonist-induced spatio-temporal change in myosin phosphorylation in smooth muscle cellsKoji Miyazaki
Department of Physiology and Biomedical Imaging Group, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Biol Chem 277:725-34. 2002..The results clearly show that the myosin light chain kinase pathway and the Rho pathway distinctly change myosin phosphorylation in smooth muscle cells in both a temporal and spatial manner... - The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagellaKarl Ferdinand Lechtreck
Department of Cell Biology, University of Massachusetts Medical School, Worcester, 01655, USA
J Cell Biol 187:1117-32. 2009..We conclude that the BBSome is carried by IFT but is an adapter rather than an integral component of the IFT machinery. C. reinhardtii BBS4 may be required for the export of signaling proteins from the flagellum via IFT... - The motor domain determines the large step of myosin-VHiroto Tanaka
Single Molecule Processes Project, ICORP, JST, 2-4-14, Senba-Higashi, Mino, Osaka 562-0035, Japan
Nature 415:192-5. 2002..These results challenge the lever-arm model. We propose that the motor domain and/or the actomyosin interface enable myosin-V to produce large processive steps during translocation along actin... - Myosin-Va regulates exocytosis through the submicromolar Ca2+-dependent binding of syntaxin-1AMichitoshi Watanabe
Division of Molecular and Cellular Biology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Niigata 951 8510, Japan
Mol Biol Cell 16:4519-30. 2005..Our results demonstrate that the interaction between myosin-Va and syntaxin-1A is involved in exocytosis and suggest that the myosin-Va neck contributes not only to the large step size but also to the regulation of exocytosis by Ca2+... - Class VI myosin moves processively along actin filaments backward with large stepsSo Nishikawa
Single Molecule Process Project, ICORP, JST, 2-4-14, Senba-Higashi, Mino, Osaka 562-0035, Japan
Biochem Biophys Res Commun 290:311-7. 2002..Myosin VI may step on these "hot spots" on actin filaments in every helical pitch, thus producing processive movement with 36 nm steps... - A one-headed class V myosin molecule develops multiple large (approximately 32-nm) steps successivelyTomonobu M Watanabe
Formation of Soft Nanomachines, Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, and Department of Biophysical Engineering, Osaka University, Suita, Osaka 565-0871, Japan
Proc Natl Acad Sci U S A 101:9630-5. 2004..The results show that a single one-headed myosin-V undergoes multiple successive large (approximately 32-nm) steps, suggesting that a novel mechanism is operating for successive myosin movement... - Unconstrained steps of myosin VI appear longest among known molecular motorsM Yusuf Ali
Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Higashiyama 5-1, Myodaiji, Okazaki 444-8787, Japan
Biophys J 86:3804-10. 2004..This mechanism may underlie stepping of all two-headed molecular motors including kinesin and myosin V... - Kinetic mechanism of the fastest motor protein, Chara myosinKohji Ito
Department of Biology, Chiba University, Inage Ku, Chiba 263 8522, Japan
J Biol Chem 282:19534-45. 2007..In addition, this study reveals some striking kinetic features of Chara myosin that are suited for the fast movement: a dramatic acceleration of ADP release by actin (1000-fold) and extremely fast ATP binding rate... - Smooth muscle myosin phosphorylated at single head shows sustained mechanical activityHiroto Tanaka
PRESTO, Japan Science and Technology Agency, 3, 5 Chiyodaku, Tokyo 102 0075, Japan
J Biol Chem 283:15611-8. 2008..We propose that the "latch state" can be explained by the motor characteristics of SHPMII that is present during the sustained phase of contraction... - Cargo-binding makes a wild-type single-headed myosin-VI move processivelyMitsuhiro Iwaki
Department of Biophysical Engineering, Osaka University, Suita, Osaka, Japan
Biophys J 90:3643-52. 2006..Although a single-headed myosin-VI was able to move processively with a large cargo, the travel distance was rather short. Multiple molecules may be involved in the cargo transport for a long travel distance in cells... - The myosin cardiac loop participates functionally in the actomyosin interactionKatalin Ajtai
Department of Biochemistry and Molecular Biology, Mayo Clinic Rochester, Rochester, Minnesota 55905, USA
J Biol Chem 279:23415-21. 2004..It appears likely that the C-loop is an essential electrostatic binding site for actin involved in modulation of actomyosin affinity and regulation of actomyosin ATPase velocity... - A unique mechanism for the processive movement of single-headed myosin-IXMasatoshi Nishikawa
Department of Biophysical Engineering, Osaka University 1 3, Machikaneyama, Toyonaka, Osaka 560 8531, Japan
Biochem Biophys Res Commun 343:1159-64. 2006..We propose that the myosin-IX head moves processively along an actin filament like an inchworm via a unique long and positively charged insertion in the loop 2 region of the head... - Myosin VI walks "wiggly" on actin with large and variable tiltingYujie Sun
Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, PA 19104, USA
Mol Cell 28:954-64. 2007.... - The diffusive search mechanism of processive myosin class-V motor involves directional steps along actin subunitsTakuya Okada
Formation of Soft Nano Machines, CREST JST, 1 3, Yamadaoka, Suita, Osaka 565 0871, Japan
Biochem Biophys Res Commun 354:379-84. 2007..These results suggest that the myosin-V head searches in the forward direction for the actin target using directional diffusion on the actin subunits according to a potential slope created along the actin helix... - Functional diversity between Rho-kinase- and MLCK-mediated cytoskeletal actions in a myofibroblast-like hepatic stellate cell lineMikio Yanase
Department of Gastroenterology, Faculty of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, 113 0033, Tokyo, Japan
Biochem Biophys Res Commun 305:223-8. 2003..These findings suggest that the actomyosin-based cytoskeletal actions can be functionally diverse depending on the Rho-kinase-mediated pathway and the MLCK-mediated pathway... - Dynamic changes in expression of myosin phosphatase in a model of portal hypertensionMichael C Payne
Departments of Medicine and Physiology, Case Western Reserve School of Medicine, Cleveland, Ohio 44106 4958, USA
Am J Physiol Heart Circ Physiol 286:H1801-10. 2004..Implications of vascular smooth muscle phenotypic diversity and reversible phenotypic modulation in portal hypertension with regards to regulation of blood flow are discussed... - Localization of telokin at the intercalated discs of cardiac myocytesGary J Kargacin
Department of Physiology and Biophysics, University of Calgary, Calgary, Alta, Canada T2N 4N1
Arch Biochem Biophys 456:151-60. 2006.... - Myosin phosphatase isoform switching in vascular smooth muscle developmentMichael C Payne
Department of Medicine, 422 BRB, 2109 Adelbert Road, Case Western Reserve School of Medicine, Cleveland, OH 44106-4958, USA
J Mol Cell Cardiol 40:274-82. 2006..We propose that MP isoform switching during neonatal vascular smooth muscle phenotypic specification may determine changing vascular responses to NO/cGMP signaling in the transition from the fetal to the adult circulation... - Biased Brownian motion mechanism for processivity and directionality of single-headed myosin-VIMitsuhiro Iwaki
Soft Biosystem Group, Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka University, 1 3, Yamadaoka, Suita, Osaka 565 871, Japan
Biosystems 93:39-47. 2008..This investigation will help us understand how molecular motors utilize Brownian motion in cells...
Research Grants
- Regulation of Myosin Phosphorylation in Smooth MuscleMitsuo Ikebe; Fiscal Year: 2010....
- Regulation of Myosin Phosphorylation in Smooth MuscleMitsuo Ikebe; Fiscal Year: 2007..To define the role of Ca2+independent MLC kinases in myosin phosphorylation upon agonist stimulation; 4. To define the localization and translocation of Ca2+independent MLC kinases and the MLCP regulatory components in smooth muscle. ..
- MOLECULAR REGULATION OF SMOOTH MUSCLE ACTOMYOSINMitsuo Ikebe; Fiscal Year: 2004..abstract_text> ..
- Regulation of Mammalian Class VI MyosinMitsuo Ikebe; Fiscal Year: 2007....
- Function of Small G-Protein Binding MyosinMitsuo Ikebe; Fiscal Year: 2007..The proposed project will clarify the function and regulation of myosin IX, a unique member of myosin super family, thus provide a new insight into the mechanism of actin based motility. ..
- Motor Function and Regulation of Myosin VIIMitsuo Ikebe; Fiscal Year: 2007..The proposed project will clarify the function and regulation of myosin VIIa, thus providing important information in understanding of the mechanism underlying the human sensory disorders of deafness and blindness. ..
- MOLECULAR REGULATION OF SMOOTH MUSCLE ACTOMYOSINMitsuo Ikebe; Fiscal Year: 1999....
- REGULATION AND FUNCTION OF MAMMALIAN MYOSIN IMitsuo Ikebe; Fiscal Year: 2002..abstract_text> ..
- PROTEIN KINASES ON SMOOTH MUSCLE REGULATIONMitsuo Ikebe; Fiscal Year: 2002..Specific aim 2. To define the role of PKC and CaMPKII on Ca2+ signaling and contraction of smooth muscle cells. Specific aim 3. To define the protein targets phosphorylated by the PKC and CaMPKIIcascade in smooth muscle cells. ..