Mitsuo Ikebe

Summary

Affiliation: University of Massachusetts Medical School
Country: USA

Publications

  1. ncbi request reprint The tip of the coiled-coil rod determines the filament formation of smooth muscle and nonmuscle myosin
    M Ikebe
    Departments of Physiology and Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 276:30293-300. 2001
  2. ncbi request reprint Conformational change and regulation of myosin molecules
    Mitsuo Ikebe
    Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Adv Exp Med Biol 565:61-72; discussion 72, 359-69. 2005
  3. ncbi request reprint Motor function of unconventional myosin
    Mitsuo Ikebe
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0127, USA
    Adv Exp Med Biol 538:143-56; discussion 157. 2003
  4. ncbi request reprint p116Rip decreases myosin II phosphorylation by activating myosin light chain phosphatase and by inactivating RhoA
    Yasuhiko Koga
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 280:4983-91. 2005
  5. ncbi request reprint A unique ATP hydrolysis mechanism of single-headed processive myosin, myosin IX
    Taketoshi Kambara
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 281:4949-57. 2006
  6. ncbi request reprint F-actin and myosin II binding domains in supervillin
    Yu Chen
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    J Biol Chem 278:46094-106. 2003
  7. ncbi request reprint Two functional heads are required for full activation of smooth muscle myosin
    Xiang Dong Li
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 278:29435-41. 2003
  8. ncbi request reprint Translocation of telokin by cGMP signaling in smooth muscle cells
    Satoshi Komatsu
    Department of Physiology, University of Massachusetts Medical School, Worcester 01655, USA
    Am J Physiol Cell Physiol 283:C752-61. 2002
  9. ncbi request reprint Human myosin III is a motor having an extremely high affinity for actin
    Taketoshi Kambara
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 281:37291-301. 2006
  10. ncbi request reprint M20, the small subunit of PP1M, binds to microtubules
    Norio Takizawa
    Department of Physiology and Biomedical Imaging Group, University of Massachusetts Medical School, Worcester 01655, USA
    Am J Physiol Cell Physiol 284:C250-62. 2003

Research Grants

  1. Regulation of Myosin Phosphorylation in Smooth Muscle
    Mitsuo Ikebe; Fiscal Year: 2010
  2. Regulation of Myosin Phosphorylation in Smooth Muscle
    Mitsuo Ikebe; Fiscal Year: 2007
  3. MOLECULAR REGULATION OF SMOOTH MUSCLE ACTOMYOSIN
    Mitsuo Ikebe; Fiscal Year: 2004
  4. Regulation of Mammalian Class VI Myosin
    Mitsuo Ikebe; Fiscal Year: 2007
  5. Function of Small G-Protein Binding Myosin
    Mitsuo Ikebe; Fiscal Year: 2007
  6. Motor Function and Regulation of Myosin VII
    Mitsuo Ikebe; Fiscal Year: 2007
  7. MOLECULAR REGULATION OF SMOOTH MUSCLE ACTOMYOSIN
    Mitsuo Ikebe; Fiscal Year: 1999
  8. REGULATION AND FUNCTION OF MAMMALIAN MYOSIN I
    Mitsuo Ikebe; Fiscal Year: 2002
  9. PROTEIN KINASES ON SMOOTH MUSCLE REGULATION
    Mitsuo Ikebe; Fiscal Year: 2002

Collaborators

Detail Information

Publications68

  1. ncbi request reprint The tip of the coiled-coil rod determines the filament formation of smooth muscle and nonmuscle myosin
    M Ikebe
    Departments of Physiology and Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 276:30293-300. 2001
    ..We conclude that the C-terminal end of the coiled-coil domain, but not the nonhelical tail piece, of myosin II is critical for myosin filament formation both in vitro and in vivo...
  2. ncbi request reprint Conformational change and regulation of myosin molecules
    Mitsuo Ikebe
    Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Adv Exp Med Biol 565:61-72; discussion 72, 359-69. 2005
  3. ncbi request reprint Motor function of unconventional myosin
    Mitsuo Ikebe
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0127, USA
    Adv Exp Med Biol 538:143-56; discussion 157. 2003
  4. ncbi request reprint p116Rip decreases myosin II phosphorylation by activating myosin light chain phosphatase and by inactivating RhoA
    Yasuhiko Koga
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 280:4983-91. 2005
    ..Based upon these findings, we propose that p116Rip is an important regulatory component that controls the RhoA signaling pathway, thus regulating MLCP activity and myosin phosphorylation in cells...
  5. ncbi request reprint A unique ATP hydrolysis mechanism of single-headed processive myosin, myosin IX
    Taketoshi Kambara
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 281:4949-57. 2006
    ..After ATP hydrolysis, Pi is quickly released to form a strong actin binding form, and a power stroke takes place...
  6. ncbi request reprint F-actin and myosin II binding domains in supervillin
    Yu Chen
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    J Biol Chem 278:46094-106. 2003
    ..Taken together, these results suggest that supervillin may mediate actin and myosin II filament organization at cholesterol-rich membrane domains...
  7. ncbi request reprint Two functional heads are required for full activation of smooth muscle myosin
    Xiang Dong Li
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 278:29435-41. 2003
    ..The present study supports an idea that the two heads of smooth muscle myosin II interact with each other and the presence of two active heads is required for full activation...
  8. ncbi request reprint Translocation of telokin by cGMP signaling in smooth muscle cells
    Satoshi Komatsu
    Department of Physiology, University of Massachusetts Medical School, Worcester 01655, USA
    Am J Physiol Cell Physiol 283:C752-61. 2002
    ..These results suggest that telokin changes its intracellular localization because of phosphorylation at Ser13 and/or Ser19 via the cGMP-signaling pathway...
  9. ncbi request reprint Human myosin III is a motor having an extremely high affinity for actin
    Taketoshi Kambara
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 281:37291-301. 2006
    ..The present results suggest that myosin IIIA can spend a majority of its ATP hydrolysis cycling time on actin...
  10. ncbi request reprint M20, the small subunit of PP1M, binds to microtubules
    Norio Takizawa
    Department of Physiology and Biomedical Imaging Group, University of Massachusetts Medical School, Worcester 01655, USA
    Am J Physiol Cell Physiol 284:C250-62. 2003
    ..These results suggest that M20 has a microtubule binding activity and plays a role in regulating microtubule dynamics...
  11. ncbi request reprint Ca2+-induced activation of ATPase activity of myosin Va is accompanied with a large conformational change
    Xiang Dong Li
    Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Biochem Biophys Res Commun 315:538-45. 2004
    ..This conformational transition is closely correlated to the actin-activated ATPase activity. These results suggest that the tail and neck domain play a role in the Ca(2+) dependent regulation of myosin Va...
  12. ncbi request reprint Myosin Va becomes a low duty ratio motor in the inhibited form
    Osamu Sato
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachueetts 01655, USA
    J Biol Chem 282:13228-39. 2007
    ....
  13. ncbi request reprint Supervillin slows cell spreading by facilitating myosin II activation at the cell periphery
    Norio Takizawa
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    J Cell Sci 120:3792-803. 2007
    ..Thus, the membrane protein SV apparently contributes to myosin II assembly during cell spreading by modulating myosin II regulation by L-MLCK...
  14. doi request reprint Effect of phosphorylation in the motor domain of human myosin IIIA on its ATP hydrolysis cycle
    Shigeru Komaba
    Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655 0127, USA
    Biochemistry 49:3695-702. 2010
    ....
  15. pmc Human myosin Vc is a low duty ratio nonprocessive motor
    Shinya Watanabe
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 283:10581-92. 2008
    ..Our findings suggest that myosin Vc fulfills its function as a cargo transporter by different mechanisms from other myosin V isoforms...
  16. pmc A novel regulatory mechanism of myosin light chain phosphorylation via binding of 14-3-3 to myosin phosphatase
    Yasuhiko Koga
    Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Mol Biol Cell 19:1062-71. 2008
    ..The present study revealed a new RhoA/Rho-kinase-dependent regulatory mechanism of myosin II phosphorylation by 14-3-3 that dissociates MLCP from myosin II and attenuates MLCP activity...
  17. ncbi request reprint Drosophila myosin VIIA is a high duty ratio motor with a unique kinetic mechanism
    Shinya Watanabe
    Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    J Biol Chem 281:7151-60. 2006
    ..9. Therefore, myosin VIIA is classified to be a high duty ratio motor. The present results suggested that myosin VIIA can be a processive motor to serve cargo trafficking in cells once it forms a dimer structure...
  18. ncbi request reprint Phosphorylation of CPI17 and myosin binding subunit of type 1 protein phosphatase by p21-activated kinase
    Norio Takizawa
    Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Biochem Biophys Res Commun 297:773-8. 2002
    ..On the other hand, the phosphorylation of MBS by PAK also decreased the MLCP activity. These results raise the possibility that the PAK pathway plays a role in MLCP regulation...
  19. pmc Head-head and head-tail interaction: a general mechanism for switching off myosin II activity in cells
    Hyun Suk Jung
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Mol Biol Cell 19:3234-42. 2008
    ..These interactions are broken when myosin is activated...
  20. ncbi request reprint Myosin X transports Mena/VASP to the tip of filopodia
    Hiroshi Tokuo
    Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Biochem Biophys Res Commun 319:214-20. 2004
    ..These results show that M10 is a specific motor carrying Mena/VASP from the root to the tip of the filopodia where extension of actin filament takes place...
  21. pmc The globular tail domain puts on the brake to stop the ATPase cycle of myosin Va
    Xiang Dong Li
    Departments of Physiology and Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Proc Natl Acad Sci U S A 105:1140-5. 2008
    ..We propose that binding of the GTD to the motor domain prevents the movement of the converter/lever arm during ATP hydrolysis cycle, thus inhibiting the chemical cycle of the motor domain...
  22. pmc Crosstalk between adenosine A1 and β1-adrenergic receptors regulates translocation of PKCε in isolated rat cardiomyocytes
    Satoshi Komatsu
    Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Cell Physiol 227:3201-7. 2012
    ..In that this translocation has been previously associated with the antiadrenergic property of A(1)R, it is apparent that the interactive effects of adenosine and β(1)-adrenergic agonists on function are complex in the heart...
  23. pmc Impacts of Usher syndrome type IB mutations on human myosin VIIa motor function
    Shinya Watanabe
    Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
    Biochemistry 47:9505-13. 2008
    ..Taken together, the results suggest that the mutations responsible for USH1B cause the complete loss of the actin-activated ATPase activity or the reduction of duty ratio of myosin VIIa...
  24. pmc The tail binds to the head-neck domain, inhibiting ATPase activity of myosin VIIA
    Nobuhisa Umeki
    Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Proc Natl Acad Sci U S A 106:8483-8. 2009
    ..We concluded that unconventional myosin that may be present a monomer in the cell can be regulated by intramolecular interaction of the tail with the head...
  25. doi request reprint Phospholipid-dependent regulation of the motor activity of myosin X
    Nobuhisa Umeki
    Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    Nat Struct Mol Biol 18:783-8. 2011
    ....
  26. pmc The motor activity of myosin-X promotes actin fiber convergence at the cell periphery to initiate filopodia formation
    Hiroshi Tokuo
    Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Cell Biol 179:229-38. 2007
    ..We conclude that the motor function of the two-headed form of myoX is critical for actin reorganization at the leading edge, leading to filopodia formation...
  27. pmc Cargo binding activates myosin VIIA motor function in cells
    Tsuyoshi Sakai
    Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Proc Natl Acad Sci U S A 108:7028-33. 2011
    ..Present findings support that MyRip, a cargo molecule, functions as an activator of myosin VIIA transporter function...
  28. ncbi request reprint BIG1 is a binding partner of myosin IXb and regulates its Rho-GTPase activating protein activity
    Nobutaka Saeki
    Department of Physiology, University of Massachusetts Medical School, 55 Lake Ave, Worcester, Massachusetts 01655, USA
    J Biol Chem 280:10128-34. 2005
    ..The findings raise a concept that the myosin transports the signaling molecule as a cargo that functions as a regulator for the myosin molecule...
  29. ncbi request reprint Myosin IXb is a single-headed minus-end-directed processive motor
    Akira Inoue
    Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655 0127, USA
    Nat Cell Biol 4:302-6. 2002
    ..The processive movement of the single-headed myosin IXb cannot be explained by a 'hand-over-hand' mechanism. This suggests that an alternative mechanism must be operating for the processive movement of single-headed myosin IXb...
  30. pmc Novel ZIP kinase isoform lacks leucine zipper
    Norio Takamoto
    Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Arch Biochem Biophys 456:194-203. 2006
    ..These results suggest that the newly found hZIPK-S as well as the long isoform play an important role in the regulation of myosin phosphorylation...
  31. ncbi request reprint Human deafness mutation of myosin VI (C442Y) accelerates the ADP dissociation rate
    Osamu Sato
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0127, USA
    J Biol Chem 279:28844-54. 2004
    ..The actin gliding velocity of the C442Y mutant decreases significantly at low surface density of myosin VI, suggesting that the mutation hampers the processive movement of myosin VI...
  32. pmc Agonist-induced changes in the phosphorylation of the myosin- binding subunit of myosin light chain phosphatase and CPI17, two regulatory factors of myosin light chain phosphatase, in smooth muscle
    Naohisa Niiro
    Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Biochem J 369:117-28. 2003
    ....
  33. ncbi request reprint Activation of myosin Va function by melanophilin, a specific docking partner of myosin Va
    Xiang Dong Li
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 280:17815-22. 2005
    ..The present finding raises the idea that myosin motors are switched upon their binding to the cargo molecules, thus avoiding the waste of ATP consumption...
  34. ncbi request reprint Myosin X is a high duty ratio motor
    Kazuaki Homma
    Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Biol Chem 280:29381-91. 2005
    ..The duty ratio calculated from our kinetic model, 0.6, was consistent with the duty ratio, 0.7, obtained from comparison of K(m ATPase) and K(m motility). Our results suggest that myosin X is a high duty ratio motor...
  35. ncbi request reprint Determination of human myosin III as a motor protein having a protein kinase activity
    Shigeru Komaba
    Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655 0127, USA
    J Biol Chem 278:21352-60. 2003
    ..The present study is the first report that clearly demonstrates that the class III myosin is an actin-based motor protein having a protein kinase activity...
  36. ncbi request reprint Dynamics of RhoA and ROKalpha translocation in single living cells
    Koji Miyazaki
    Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Cell Biochem Biophys 45:243-54. 2006
    ....
  37. ncbi request reprint The globular tail domain of myosin Va functions as an inhibitor of the myosin Va motor
    Xiang Dong Li
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 281:21789-98. 2006
    ....
  38. pmc Adenoprotection of the heart involves phospholipase C-induced activation and translocation of PKC-epsilon to RACK2 in adult rat and mouse
    Richard A Fenton
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Am J Physiol Heart Circ Physiol 297:H718-25. 2009
    ..In conclusion, A(1)R stimulation of the heart is associated with PLC-initiated PKC-epsilon translocation and association with RACK2...
  39. ncbi request reprint Mechanoenzymatic characterization of human myosin Vb
    Shinya Watanabe
    Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655 0127, USA
    Biochemistry 45:2729-38. 2006
    ..22 +/- 0.03 microm/s) remained constant at a low motor density. The actin filament landing assay revealed that a single HuM5B molecule is sufficient to move the actin filament continuously, indicating that HuM5b is a processive motor...
  40. ncbi request reprint cGMP-dependent relaxation of smooth muscle is coupled with the change in the phosphorylation of myosin phosphatase
    Kensei Nakamura
    University of Massachusetts Medical School, Department of Physiology, 55 Lake Avenue North, Worcester, MA 01655, USA
    Circ Res 101:712-22. 2007
    ..This results in the decrease in MLC phosphorylation and smooth muscle relaxation...
  41. pmc ZIP kinase is responsible for the phosphorylation of myosin II and necessary for cell motility in mammalian fibroblasts
    Satoshi Komatsu
    Dept of Physiology, University of Massachusetts Medical School, 55 Lake Ave North, Worcester, MA 01655, USA
    J Cell Biol 165:243-54. 2004
    ..These results suggest that ZIP kinase is critical for myosin phosphorylation and necessary for cell motile processes in mammalian fibroblasts...
  42. ncbi request reprint DOC-2/DAB2 is the binding partner of myosin VI
    Akira Inoue
    Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655 0127, USA
    Biochem Biophys Res Commun 292:300-7. 2002
    ..The present findings suggest that myosin VI plays a role in transporting DOC-2/DAB2, a Ras cascade signaling molecule, thus involved in Ras signaling pathways...
  43. ncbi request reprint Protein kinase Cepsilon and the antiadrenergic action of adenosine in rat ventricular myocytes
    Koji Miyazaki
    Dept of Physiology, S4 242, University of Massachusetts Medical School, 55 Lake Avenue N, Worcester, MA 01655, USA
    Am J Physiol Heart Circ Physiol 287:H1721-9. 2004
    ..These results suggest that the translocation of PKCepsilon to the t-tubular system plays an important role in A(1)R-mediated antiadrenergic actions in the heart...
  44. ncbi request reprint Characterization of the motor activity of mammalian myosin VIIA
    Akira Inoue
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0127, USA
    J Biol Chem 278:5478-87. 2003
    ..The directionality of myosin VIIA was determined by using the polarity-marked dual fluorescence-labeled actin filaments. It was found that myosin VIIA is a plus-directed motor...
  45. pmc The cellular and molecular basis of bitter tastant-induced bronchodilation
    Cheng Hai Zhang
    Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America
    PLoS Biol 11:e1001501. 2013
    ..We propose that the large decrease in [Ca(2+)]i caused by effective tastant bronchodilators provides an efficient cell-based screening method for identifying potent dilators from among the many thousands of available bitter tastants...
  46. pmc The phosphorylation of myosin II at the Ser1 and Ser2 is critical for normal platelet-derived growth factor induced reorganization of myosin filaments
    Satoshi Komatsu
    Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Mol Biol Cell 18:5081-90. 2007
    ....
  47. doi request reprint Regulation of the function of mammalian myosin and its conformational change
    Mitsuo Ikebe
    Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Biochem Biophys Res Commun 369:157-64. 2008
    ..These findings suggest the presence of a common scenario for the regulation of motor protein functions...
  48. pmc Human airway contraction and formoterol-induced relaxation is determined by Ca2+ oscillations and Ca2+ sensitivity
    Anna Rebekka Ressmeyer
    Department of Physiology, University of Massachusetts Medical School, Worcester, 01655, USA
    Am J Respir Cell Mol Biol 43:179-91. 2010
    ..Formoterol induces long-lasting relaxation by initially reducing the Ca(2+) sensitivity and, subsequently, the frequency of the Ca(2+) oscillations of the airway SMCs...
  49. ncbi request reprint Dephosphorylation of the two regulatory components of myosin phosphatase, MBS and CPI17
    Norio Takizawa
    Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    FEBS Lett 515:127-32. 2002
    ..On the other hand, phosphorylated CPI17 is readily dephosphorylated thus likely to play a role in regulating fast phosphorylation-dephosphorylation cycle in cells...
  50. ncbi request reprint Rho-dependent agonist-induced spatio-temporal change in myosin phosphorylation in smooth muscle cells
    Koji Miyazaki
    Department of Physiology and Biomedical Imaging Group, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 277:725-34. 2002
    ..The results clearly show that the myosin light chain kinase pathway and the Rho pathway distinctly change myosin phosphorylation in smooth muscle cells in both a temporal and spatial manner...
  51. pmc The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella
    Karl Ferdinand Lechtreck
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, 01655, USA
    J Cell Biol 187:1117-32. 2009
    ..We conclude that the BBSome is carried by IFT but is an adapter rather than an integral component of the IFT machinery. C. reinhardtii BBS4 may be required for the export of signaling proteins from the flagellum via IFT...
  52. ncbi request reprint Kinetic mechanism of the fastest motor protein, Chara myosin
    Kohji Ito
    Department of Biology, Chiba University, Inage Ku, Chiba 263 8522, Japan
    J Biol Chem 282:19534-45. 2007
    ..In addition, this study reveals some striking kinetic features of Chara myosin that are suited for the fast movement: a dramatic acceleration of ADP release by actin (1000-fold) and extremely fast ATP binding rate...
  53. ncbi request reprint Class VI myosin moves processively along actin filaments backward with large steps
    So Nishikawa
    Single Molecule Process Project, ICORP, JST, 2 4 14, Senba Higashi, Mino, Osaka 562 0035, Japan
    Biochem Biophys Res Commun 290:311-7. 2002
    ..Myosin VI may step on these "hot spots" on actin filaments in every helical pitch, thus producing processive movement with 36 nm steps...
  54. ncbi request reprint The motor domain determines the large step of myosin-V
    Hiroto Tanaka
    Single Molecule Processes Project, ICORP, JST, 2 4 14, Senba Higashi, Mino, Osaka 562 0035, Japan
    Nature 415:192-5. 2002
    ..These results challenge the lever-arm model. We propose that the motor domain and/or the actomyosin interface enable myosin-V to produce large processive steps during translocation along actin...
  55. pmc Myosin-Va regulates exocytosis through the submicromolar Ca2+-dependent binding of syntaxin-1A
    Michitoshi Watanabe
    Division of Molecular and Cellular Biology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Niigata 951 8510, Japan
    Mol Biol Cell 16:4519-30. 2005
    ..Our results demonstrate that the interaction between myosin-Va and syntaxin-1A is involved in exocytosis and suggest that the myosin-Va neck contributes not only to the large step size but also to the regulation of exocytosis by Ca2+...
  56. ncbi request reprint The diffusive search mechanism of processive myosin class-V motor involves directional steps along actin subunits
    Takuya Okada
    Formation of Soft Nano Machines, CREST JST, 1 3, Yamadaoka, Suita, Osaka 565 0871, Japan
    Biochem Biophys Res Commun 354:379-84. 2007
    ..These results suggest that the myosin-V head searches in the forward direction for the actin target using directional diffusion on the actin subunits according to a potential slope created along the actin helix...
  57. pmc A one-headed class V myosin molecule develops multiple large (approximately 32-nm) steps successively
    Tomonobu M Watanabe
    Formation of Soft Nanomachines, Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, and Department of Biophysical Engineering, Osaka University, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 101:9630-5. 2004
    ..The results show that a single one-headed myosin-V undergoes multiple successive large (approximately 32-nm) steps, suggesting that a novel mechanism is operating for successive myosin movement...
  58. pmc Unconstrained steps of myosin VI appear longest among known molecular motors
    M Yusuf Ali
    Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Higashiyama 5 1, Myodaiji, Okazaki 444 8787, Japan
    Biophys J 86:3804-10. 2004
    ..This mechanism may underlie stepping of all two-headed molecular motors including kinesin and myosin V...
  59. ncbi request reprint Functional diversity between Rho-kinase- and MLCK-mediated cytoskeletal actions in a myofibroblast-like hepatic stellate cell line
    Mikio Yanase
    Department of Gastroenterology, Faculty of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, 113 0033, Tokyo, Japan
    Biochem Biophys Res Commun 305:223-8. 2003
    ..These findings suggest that the actomyosin-based cytoskeletal actions can be functionally diverse depending on the Rho-kinase-mediated pathway and the MLCK-mediated pathway...
  60. ncbi request reprint The myosin cardiac loop participates functionally in the actomyosin interaction
    Katalin Ajtai
    Department of Biochemistry and Molecular Biology, Mayo Clinic Rochester, Rochester, Minnesota 55905, USA
    J Biol Chem 279:23415-21. 2004
    ..It appears likely that the C-loop is an essential electrostatic binding site for actin involved in modulation of actomyosin affinity and regulation of actomyosin ATPase velocity...
  61. pmc Smooth muscle myosin phosphorylated at single head shows sustained mechanical activity
    Hiroto Tanaka
    PRESTO, Japan Science and Technology Agency, 3, 5 Chiyodaku, Tokyo 102 0075, Japan
    J Biol Chem 283:15611-8. 2008
    ..We propose that the "latch state" can be explained by the motor characteristics of SHPMII that is present during the sustained phase of contraction...
  62. pmc Cargo-binding makes a wild-type single-headed myosin-VI move processively
    Mitsuhiro Iwaki
    Department of Biophysical Engineering, Osaka University, Suita, Osaka, Japan
    Biophys J 90:3643-52. 2006
    ..Although a single-headed myosin-VI was able to move processively with a large cargo, the travel distance was rather short. Multiple molecules may be involved in the cargo transport for a long travel distance in cells...
  63. ncbi request reprint A unique mechanism for the processive movement of single-headed myosin-IX
    Masatoshi Nishikawa
    Department of Biophysical Engineering, Osaka University 1 3, Machikaneyama, Toyonaka, Osaka 560 8531, Japan
    Biochem Biophys Res Commun 343:1159-64. 2006
    ..We propose that the myosin-IX head moves processively along an actin filament like an inchworm via a unique long and positively charged insertion in the loop 2 region of the head...
  64. pmc Myosin VI walks "wiggly" on actin with large and variable tilting
    Yujie Sun
    Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, PA 19104, USA
    Mol Cell 28:954-64. 2007
    ....
  65. doi request reprint Biased Brownian motion mechanism for processivity and directionality of single-headed myosin-VI
    Mitsuhiro Iwaki
    Soft Biosystem Group, Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka University, 1 3, Yamadaoka, Suita, Osaka 565 871, Japan
    Biosystems 93:39-47. 2008
    ..This investigation will help us understand how molecular motors utilize Brownian motion in cells...
  66. ncbi request reprint Localization of telokin at the intercalated discs of cardiac myocytes
    Gary J Kargacin
    Department of Physiology and Biophysics, University of Calgary, Calgary, Alta, Canada T2N 4N1
    Arch Biochem Biophys 456:151-60. 2006
    ....
  67. ncbi request reprint Myosin phosphatase isoform switching in vascular smooth muscle development
    Michael C Payne
    Department of Medicine, 422 BRB, 2109 Adelbert Road, Case Western Reserve School of Medicine, Cleveland, OH 44106 4958, USA
    J Mol Cell Cardiol 40:274-82. 2006
    ..We propose that MP isoform switching during neonatal vascular smooth muscle phenotypic specification may determine changing vascular responses to NO/cGMP signaling in the transition from the fetal to the adult circulation...
  68. ncbi request reprint Dynamic changes in expression of myosin phosphatase in a model of portal hypertension
    Michael C Payne
    Departments of Medicine and Physiology, Case Western Reserve School of Medicine, Cleveland, Ohio 44106 4958, USA
    Am J Physiol Heart Circ Physiol 286:H1801-10. 2004
    ..Implications of vascular smooth muscle phenotypic diversity and reversible phenotypic modulation in portal hypertension with regards to regulation of blood flow are discussed...

Research Grants40

  1. Regulation of Myosin Phosphorylation in Smooth Muscle
    Mitsuo Ikebe; Fiscal Year: 2010
    ....
  2. Regulation of Myosin Phosphorylation in Smooth Muscle
    Mitsuo Ikebe; Fiscal Year: 2007
    ..To define the role of Ca2+independent MLC kinases in myosin phosphorylation upon agonist stimulation; 4. To define the localization and translocation of Ca2+independent MLC kinases and the MLCP regulatory components in smooth muscle. ..
  3. MOLECULAR REGULATION OF SMOOTH MUSCLE ACTOMYOSIN
    Mitsuo Ikebe; Fiscal Year: 2004
    ..abstract_text> ..
  4. Regulation of Mammalian Class VI Myosin
    Mitsuo Ikebe; Fiscal Year: 2007
    ....
  5. Function of Small G-Protein Binding Myosin
    Mitsuo Ikebe; Fiscal Year: 2007
    ..The proposed project will clarify the function and regulation of myosin IX, a unique member of myosin super family, thus provide a new insight into the mechanism of actin based motility. ..
  6. Motor Function and Regulation of Myosin VII
    Mitsuo Ikebe; Fiscal Year: 2007
    ..The proposed project will clarify the function and regulation of myosin VIIa, thus providing important information in understanding of the mechanism underlying the human sensory disorders of deafness and blindness. ..
  7. MOLECULAR REGULATION OF SMOOTH MUSCLE ACTOMYOSIN
    Mitsuo Ikebe; Fiscal Year: 1999
    ....
  8. REGULATION AND FUNCTION OF MAMMALIAN MYOSIN I
    Mitsuo Ikebe; Fiscal Year: 2002
    ..abstract_text> ..
  9. PROTEIN KINASES ON SMOOTH MUSCLE REGULATION
    Mitsuo Ikebe; Fiscal Year: 2002
    ..Specific aim 2. To define the role of PKC and CaMPKII on Ca2+ signaling and contraction of smooth muscle cells. Specific aim 3. To define the protein targets phosphorylated by the PKC and CaMPKIIcascade in smooth muscle cells. ..