Edward L Huttlin

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. ncbi request reprint Comparison of full versus partial metabolic labeling for quantitative proteomics analysis in Arabidopsis thaliana
    Edward L Huttlin
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    Mol Cell Proteomics 6:860-81. 2007
  2. pmc Discovery and validation of colonic tumor-associated proteins via metabolic labeling and stable isotopic dilution
    Edward L Huttlin
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 106:17235-40. 2009
  3. ncbi request reprint Implications of 15N-metabolic labeling for automated peptide identification in Arabidopsis thaliana
    Clark J Nelson
    Department of Comparative Biosciences, University of Wisconsin, Madison, WI, USA
    Proteomics 7:1279-92. 2007
  4. pmc Candidate serum biomarkers for early intestinal cancer using 15N metabolic labeling and quantitative proteomics in the ApcMin/+ mouse
    Melanie M Ivancic
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, United States
    J Proteome Res 12:4152-66. 2013
  5. ncbi request reprint Stable isotope assisted assignment of elemental compositions for metabolomics
    Adrian D Hegeman
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin 53706, USA
    Anal Chem 79:6912-21. 2007
  6. pmc Prediction of error associated with false-positive rate determination for peptide identification in large-scale proteomics experiments using a combined reverse and forward peptide sequence database strategy
    Edward L Huttlin
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin 53706, USA
    J Proteome Res 6:392-8. 2007
  7. pmc Matching isotopic distributions from metabolically labeled samples
    Sean McIlwain
    Department of Computer Sciences, University of Wisconsin, Madison, WI, USA
    Bioinformatics 24:i339-47. 2008
  8. doi request reprint Democratization and integration of genomic profiling tools
    Michael R Sussman
    Department of Biochemistry, UW Biotechnology Center, University of Wisconsin Madison, Madison, WI, USA
    Methods Mol Biol 553:373-93. 2009
  9. ncbi request reprint Using dynamic programming to create isotopic distribution maps from mass spectra
    Sean McIlwain
    Department of Computer Sciences, University of Wisconsin, Madison, WI, USA
    Bioinformatics 23:i328-36. 2007
  10. pmc A transcriptome-based characterization of habituation in plant tissue culture
    Melissa S Pischke
    University of Wisconsin Biotechnology Center and Department of Biochemistry, Madison, Wisconsin 53706, USA
    Plant Physiol 140:1255-78. 2006

Collaborators

Detail Information

Publications10

  1. ncbi request reprint Comparison of full versus partial metabolic labeling for quantitative proteomics analysis in Arabidopsis thaliana
    Edward L Huttlin
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    Mol Cell Proteomics 6:860-81. 2007
    ..This difference is especially pronounced at extreme ratios. Ultimately both full metabolic labeling and partial metabolic labeling prove to be well suited for quantitative proteomics characterization...
  2. pmc Discovery and validation of colonic tumor-associated proteins via metabolic labeling and stable isotopic dilution
    Edward L Huttlin
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 106:17235-40. 2009
    ..Our work demonstrates the power of metabolic labeling combined with stable isotopic dilution as an integrated strategy for the identification and validation of differentially expressed proteins using rodent models of human disease...
  3. ncbi request reprint Implications of 15N-metabolic labeling for automated peptide identification in Arabidopsis thaliana
    Clark J Nelson
    Department of Comparative Biosciences, University of Wisconsin, Madison, WI, USA
    Proteomics 7:1279-92. 2007
    ..However, by determining the nitrogen count from each pair of labeled and unlabeled peptides we may improve our confidence in both heavy and light identifications...
  4. pmc Candidate serum biomarkers for early intestinal cancer using 15N metabolic labeling and quantitative proteomics in the ApcMin/+ mouse
    Melanie M Ivancic
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, United States
    J Proteome Res 12:4152-66. 2013
    ..These differentially expressed proteins identified in the ApcMin/+ mouse model have provided a set of candidate biomarkers for future validation screens in humans...
  5. ncbi request reprint Stable isotope assisted assignment of elemental compositions for metabolomics
    Adrian D Hegeman
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin 53706, USA
    Anal Chem 79:6912-21. 2007
    ..These results demonstrate that metabolic labeling can be used to provide additional constraints for higher confidence formula assignments over an extended mass range...
  6. pmc Prediction of error associated with false-positive rate determination for peptide identification in large-scale proteomics experiments using a combined reverse and forward peptide sequence database strategy
    Edward L Huttlin
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin 53706, USA
    J Proteome Res 6:392-8. 2007
    ..Finally, we consider how these approaches may be applied to more complicated data sets, as when peptides are separated by charge state prior to false-positive determination...
  7. pmc Matching isotopic distributions from metabolically labeled samples
    Sean McIlwain
    Department of Computer Sciences, University of Wisconsin, Madison, WI, USA
    Bioinformatics 24:i339-47. 2008
    ..Such a method is needed for high-throughput quantitative proteomic metabolomic experiments measured via mass spectrometry...
  8. doi request reprint Democratization and integration of genomic profiling tools
    Michael R Sussman
    Department of Biochemistry, UW Biotechnology Center, University of Wisconsin Madison, Madison, WI, USA
    Methods Mol Biol 553:373-93. 2009
    ..In this chapter we provide a review and evaluation of several genomic profiling techniques used in plant systems biology as well as make recommendations for future progress in their use and integration...
  9. ncbi request reprint Using dynamic programming to create isotopic distribution maps from mass spectra
    Sean McIlwain
    Department of Computer Sciences, University of Wisconsin, Madison, WI, USA
    Bioinformatics 23:i328-36. 2007
    ..Such a system is needed for a variety of purposes, including generating robust and meaningful features from mass spectra to be used in classification...
  10. pmc A transcriptome-based characterization of habituation in plant tissue culture
    Melissa S Pischke
    University of Wisconsin Biotechnology Center and Department of Biochemistry, Madison, Wisconsin 53706, USA
    Plant Physiol 140:1255-78. 2006
    ....