Christopher C Hughes

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint Diffusion limits of an in vitro thick prevascularized tissue
    Craig K Griffith
    Department of Biomedical Engineering, University of California, Irvine, Irvine, California 92697 2715, USA
    Tissue Eng 11:257-66. 2005
  2. pmc Integration of the beta-catenin-dependent Wnt pathway with integrin signaling through the adaptor molecule Grb2
    Steve P Crampton
    Department of Molecular Biology and Biochemistry, UCI Institute for Immunology, University of California Irvine, Irvine, California, USA
    PLoS ONE 4:e7841. 2009
  3. pmc Analysis of stromal cell secretomes reveals a critical role for stromal cell-derived hepatocyte growth factor and fibronectin in angiogenesis
    Andrew C Newman
    Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, CA 92697, USA
    Arterioscler Thromb Vasc Biol 33:513-22. 2013
  4. pmc Macrophages and angiogenesis: a role for Wnt signaling
    Andrew C Newman
    The Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, CA, 92697, USA
    Vasc Cell 4:13. 2012
  5. doi request reprint Endothelial-stromal interactions in angiogenesis
    Christopher C W Hughes
    Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, California 92697, USA
    Curr Opin Hematol 15:204-9. 2008
  6. ncbi request reprint Mesenchymal stem cells enhance angiogenesis in mechanically viable prevascularized tissues via early matrix metalloproteinase upregulation
    Cyrus M Ghajar
    Department of Biomedical Engineering, University of California Irvine, Irvine, California 92697 2575, USA
    Tissue Eng 12:2875-88. 2006
  7. pmc Rapid anastomosis of endothelial progenitor cell-derived vessels with host vasculature is promoted by a high density of cotransplanted fibroblasts
    Xiaofang Chen
    Department of Biomedical Engineering, University of California, Irvine, Irvine, California 92697 2730, USA
    Tissue Eng Part A 16:585-94. 2010
  8. doi request reprint An optimized three-dimensional in vitro model for the analysis of angiogenesis
    Martin N Nakatsu
    Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, California, USA
    Methods Enzymol 443:65-82. 2008
  9. doi request reprint Crosstalk between vascular endothelial growth factor, notch, and transforming growth factor-beta in vascular morphogenesis
    Matthew T Holderfield
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
    Circ Res 102:637-52. 2008
  10. pmc Prevascularization of a fibrin-based tissue construct accelerates the formation of functional anastomosis with host vasculature
    Xiaofang Chen
    Department of Biomedical Engineering, University of California Irvine, Irvine, California 92697 2715, USA
    Tissue Eng Part A 15:1363-71. 2009

Research Grants

  1. Endothelial Cell Regulation of T Cell Activation
    Christopher Hughes; Fiscal Year: 2005
  2. MOLECULAR APPROACHES TARGETED ON BLOCKING ANGIOGENESIS
    Christopher Hughes; Fiscal Year: 2006
  3. Bidirectional Notch Signaling and Angiogenesis
    Christopher Hughes; Fiscal Year: 2007
  4. Bidirectional Notch Signaling and Angiogenesis
    Christopher Hughes; Fiscal Year: 2009
  5. Bidirectional Notch Signaling and Angiogenesis
    Christopher C W Hughes; Fiscal Year: 2010
  6. MOLECULAR APPROACHES TARGETED ON BLOCKING ANGIOGENESIS
    Christopher Hughes; Fiscal Year: 2002
  7. ENDOTHELIAL REGULATION OF T CELL TRANSCRIPTION FACTORS
    Christopher Hughes; Fiscal Year: 2000
  8. MOLECULAR APPROACHES TARGETED ON BLOCKING ANGIOGENESIS
    Christopher C W Hughes; Fiscal Year: 2010

Collaborators

  • Richard C A Sainson
  • Stephen P Arold
  • Cyrus M Ghajar
  • Martin N Nakatsu
  • Andrew C Newman
  • Xiaofang Chen
  • Steve P Crampton
  • Matthew T Holderfield
  • Steven C George
  • Beibei Wu
  • Mark Aitkenhead
  • Kevin L Taylor
  • Craig K Griffith
  • Stephanie A Popson
  • Javier Mestas
  • Anna S Aledia
  • Douglas A Johnston
  • Sofía Pérez-Del-Pulgar
  • Philip M Carpenter
  • Jason N Aoto
  • Katrina M Welch-Reardon
  • Duc Thien Phan
  • Paul D Gershon
  • Dananh P Nguyen
  • Wayne Chou
  • Ashley H Fong
  • Daniel R Sandoval
  • Linda Him
  • Andrew J Putnam
  • Jai Hyun Kim
  • Marian L Waterman
  • Candice Solomon
  • Edward J Park
  • Bamboo Dong
  • Michael T Chin
  • April M Henderson Anderson
  • Randy L Johnson
  • Hiroki Kokubo
  • Noo Li Jeon
  • Cheryl Miller
  • Toshiyuki Hori
  • Jay W Calvert
  • Shur Jen Wang
  • April M Henderson
  • Cheryl Heard

Detail Information

Publications21

  1. ncbi request reprint Diffusion limits of an in vitro thick prevascularized tissue
    Craig K Griffith
    Department of Biomedical Engineering, University of California, Irvine, Irvine, California 92697 2715, USA
    Tissue Eng 11:257-66. 2005
    ..In addition, diffusion of fibroblast-derived soluble mediators is necessary for stable capillary formation, but is significantly impeded relative to that of nutrients present in the medium...
  2. pmc Integration of the beta-catenin-dependent Wnt pathway with integrin signaling through the adaptor molecule Grb2
    Steve P Crampton
    Department of Molecular Biology and Biochemistry, UCI Institute for Immunology, University of California Irvine, Irvine, California, USA
    PLoS ONE 4:e7841. 2009
    ..Both rac1 and c-jun have recently been implicated in wnt signaling, however their upstream activators have not been identified...
  3. pmc Analysis of stromal cell secretomes reveals a critical role for stromal cell-derived hepatocyte growth factor and fibronectin in angiogenesis
    Andrew C Newman
    Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, CA 92697, USA
    Arterioscler Thromb Vasc Biol 33:513-22. 2013
    ..Angiogenesis requires tightly coordinated crosstalk between endothelial cells (ECs) and stromal cells, such as fibroblasts and smooth muscle cells. The specific molecular mechanisms moderating this process are still poorly understood...
  4. pmc Macrophages and angiogenesis: a role for Wnt signaling
    Andrew C Newman
    The Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, CA, 92697, USA
    Vasc Cell 4:13. 2012
    ..Finally, we propose that Wnt signaling plays a central role in differentiation of tumor associated and wound infiltrating macrophages to a proangiogenic phenotype...
  5. doi request reprint Endothelial-stromal interactions in angiogenesis
    Christopher C W Hughes
    Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, California 92697, USA
    Curr Opin Hematol 15:204-9. 2008
    ..This review will focus on the recent findings on the interactions between angiogenic endothelial cells and the other components of the stroma - fibroblasts, pericytes and extracellular matrix...
  6. ncbi request reprint Mesenchymal stem cells enhance angiogenesis in mechanically viable prevascularized tissues via early matrix metalloproteinase upregulation
    Cyrus M Ghajar
    Department of Biomedical Engineering, University of California Irvine, Irvine, California 92697 2575, USA
    Tissue Eng 12:2875-88. 2006
    ..This study provides a means to design stiffer prevascularized tissues utilizing naturally derived substrates, and its results may yield new mechanistic insights into stem cell-based angiogenic therapies...
  7. pmc Rapid anastomosis of endothelial progenitor cell-derived vessels with host vasculature is promoted by a high density of cotransplanted fibroblasts
    Xiaofang Chen
    Department of Biomedical Engineering, University of California, Irvine, Irvine, California 92697 2730, USA
    Tissue Eng Part A 16:585-94. 2010
    ....
  8. doi request reprint An optimized three-dimensional in vitro model for the analysis of angiogenesis
    Martin N Nakatsu
    Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, California, USA
    Methods Enzymol 443:65-82. 2008
    ....
  9. doi request reprint Crosstalk between vascular endothelial growth factor, notch, and transforming growth factor-beta in vascular morphogenesis
    Matthew T Holderfield
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
    Circ Res 102:637-52. 2008
    ....
  10. pmc Prevascularization of a fibrin-based tissue construct accelerates the formation of functional anastomosis with host vasculature
    Xiaofang Chen
    Department of Biomedical Engineering, University of California Irvine, Irvine, California 92697 2715, USA
    Tissue Eng Part A 15:1363-71. 2009
    ..Our prevascularization strategy may be useful to design large three-dimensional engineered tissues...
  11. pmc TNF primes endothelial cells for angiogenic sprouting by inducing a tip cell phenotype
    Richard C A Sainson
    Department of Molecular Biology and Biochemistry, University of California, Irvine, USA
    Blood 111:4997-5007. 2008
    ....
  12. pmc The effect of matrix density on the regulation of 3-D capillary morphogenesis
    Cyrus M Ghajar
    Department of Biomedical Engineering, University of California, Irvine, Irvine, California 92697, USA
    Biophys J 94:1930-41. 2008
    ..Although roles for matrix stiffness and ligand binding density have previously been identified, our results emphasize the importance of diffusion restrictions in limiting capillary morphogenesis...
  13. ncbi request reprint Angiogenic sprouting and capillary lumen formation modeled by human umbilical vein endothelial cells (HUVEC) in fibrin gels: the role of fibroblasts and Angiopoietin-1
    Martin N Nakatsu
    Department of Molecular Biology and Biochemistry, 3219 McGaugh Hall, University of California, Irvine, Irvine, CA 92697, USA
    Microvasc Res 66:102-12. 2003
    ..This improved model recapitulates all the major steps of angiogenesis seen in vivo and provides a powerful model for analysis of this complex phenomenon...
  14. ncbi request reprint Identification of endothelial cell genes expressed in an in vitro model of angiogenesis: induction of ESM-1, (beta)ig-h3, and NrCAM
    Mark Aitkenhead
    Molecular Biology and Biochemistry, University of California at Irvine, Irvine, California 92697, USA
    Microvasc Res 63:159-71. 2002
    ..These data expand the suite of genes implicated in vascular remodeling and angiogenesis...
  15. ncbi request reprint Cell-autonomous notch signaling regulates endothelial cell branching and proliferation during vascular tubulogenesis
    Richard C A Sainson
    Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, California 92612, USA
    FASEB J 19:1027-9. 2005
    ..Thus, notch is used reiteratively and cell-autonomously by EC to regulate vessel diameter, to limit branching at the tip of sprouts, and to establish a mature, quiescent phenotype...
  16. ncbi request reprint Endothelial cell co-stimulation through OX40 augments and prolongs T cell cytokine synthesis by stabilization of cytokine mRNA
    Javier Mestas
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
    Int Immunol 17:737-47. 2005
    ..Thus, EC co-stimulation through OX40 leads to prolonged T cell cytokine synthesis and enhanced proliferation...
  17. ncbi request reprint HESR1/CHF2 suppresses VEGFR2 transcription independent of binding to E-boxes
    Matthew T Holderfield
    Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, CA 92697, USA
    Biochem Biophys Res Commun 346:637-48. 2006
    ..Thus, HESR1 represses VEGFR2 through interactions with SP-1-like factors and requires an Inr element in the absence of a TATA box. Our findings illuminate an important mechanism for notch/HESR1 regulation of VEGF-induced angiogenesis...
  18. ncbi request reprint VEGF(121) and VEGF(165) regulate blood vessel diameter through vascular endothelial growth factor receptor 2 in an in vitro angiogenesis model
    Martin N Nakatsu
    Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, California 92697, USA
    Lab Invest 83:1873-85. 2003
    ..We conclude that the diameter of new capillary sprouts can be determined by the local concentration of VEGF and that the action of VEGF on angiogenic EC in this assay is critically dependent on signaling through VEGFR-2...
  19. pmc Wnt signaling induces matrix metalloproteinase expression and regulates T cell transmigration
    Beibei Wu
    Center for Immunology, Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, CA 92697, USA
    Immunity 26:227-39. 2007
    ..Wnt signaling stabilizes beta-catenin protein in T cells and directly targets the MMP promoters through tandem TCF sites. Thus, our data support a necessary and previously unexpected role for wnt signaling in T cell extravasation...
  20. ncbi request reprint Notch activation during endothelial cell network formation in vitro targets the basic HLH transcription factor HESR-1 and downregulates VEGFR-2/KDR expression
    Kevin L Taylor
    Dept of Molecular Biology and Biochemistry, University of California Irvine, Irvine, CA 92627 3900, USA
    Microvasc Res 64:372-83. 2002
    ..These data suggest that HESR-1 may be involved in the phenotypic changes that characterize the progression from EC proliferation and migration to network formation and quiescence...
  21. pmc TNF induction of jagged-1 in endothelial cells is NFkappaB-dependent
    Douglas A Johnston
    Department of Molecular Biology and Biochemistry, University of California Irvine, 92697, USA
    Gene 435:36-44. 2009
    ..Full promoter activity also depends on an AP-1 site at -2.0 kb. These results indicate that canonical NFkappaB signaling is required for TNF induction of the notch ligand jagged-1 in EC...

Research Grants24

  1. Endothelial Cell Regulation of T Cell Activation
    Christopher Hughes; Fiscal Year: 2005
    ..Completion of these aims will provide us with a better understanding of the role of EC in shaping immune responses, and may open avenues for design of therapies that reduce the severity of graft arteriosclerosis. ..
  2. MOLECULAR APPROACHES TARGETED ON BLOCKING ANGIOGENESIS
    Christopher Hughes; Fiscal Year: 2006
    ..Our hope is that these studies will furnish a more thorough understanding of the genes regulating blood vessel patterning during angiogenesis. ..
  3. Bidirectional Notch Signaling and Angiogenesis
    Christopher Hughes; Fiscal Year: 2007
    ..Completion of this study will not only provide us with fundamental knowledge about how the body works, but it will also provide us with new information that will help in the fight against cancer. ..
  4. Bidirectional Notch Signaling and Angiogenesis
    Christopher Hughes; Fiscal Year: 2009
    ..Completion of this study will not only provide us with fundamental knowledge about how the body works, but it will also provide us with new information that will help in the fight against cancer. ..
  5. Bidirectional Notch Signaling and Angiogenesis
    Christopher C W Hughes; Fiscal Year: 2010
    ..Completion of this study will not only provide us with fundamental knowledge about how the body works, but it will also provide us with new information that will help in the fight against cancer. ..
  6. MOLECULAR APPROACHES TARGETED ON BLOCKING ANGIOGENESIS
    Christopher Hughes; Fiscal Year: 2002
    ..abstract_text> ..
  7. ENDOTHELIAL REGULATION OF T CELL TRANSCRIPTION FACTORS
    Christopher Hughes; Fiscal Year: 2000
    ....
  8. MOLECULAR APPROACHES TARGETED ON BLOCKING ANGIOGENESIS
    Christopher C W Hughes; Fiscal Year: 2010
    ..In this project we will learn more about how these molecules work in the hope that we may manipulate them in the future to control blood vessel development. ..