Wayne Hubbell

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Technological advances in site-directed spin labeling of proteins
    Wayne L Hubbell
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, United States Electronic address
    Curr Opin Struct Biol 23:725-33. 2013
  2. ncbi request reprint Watching proteins move using site-directed spin labeling
    W L Hubbell
    Jules Stein Eye Institute, University of California at Los Angeles 90095 7008, USA
    Structure 4:779-83. 1996
  3. ncbi request reprint Recent advances in site-directed spin labeling of proteins
    W L Hubbell
    Jules Stein Eye Institute, University of California, Los Angeles 90095, USA
    Curr Opin Struct Biol 8:649-56. 1998
  4. ncbi request reprint Identifying conformational changes with site-directed spin labeling
    W L Hubbell
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, USA
    Nat Struct Biol 7:735-9. 2000
  5. ncbi request reprint Structure and function in rhodopsin: mapping light-dependent changes in distance between residue 316 in helix 8 and residues in the sequence 60-75, covering the cytoplasmic end of helices TM1 and TM2 and their connection loop CL1
    C Altenbach
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-7008, USA
    Biochemistry 40:15493-500. 2001
  6. ncbi request reprint Helix packing in the lactose permease of Escherichia coli: distances between site-directed nitroxides and a lanthanide
    J Voss
    Chemistry Department, University of California, Davis, California 95616 0935, USA
    Biochemistry 40:3184-8. 2001
  7. ncbi request reprint Molecular motion of spin labeled side chains in alpha-helices: analysis by variation of side chain structure
    L Columbus
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-7008, USA
    Biochemistry 40:3828-46. 2001
  8. ncbi request reprint Structure and function in rhodopsin: mapping light-dependent changes in distance between residue 65 in helix TM1 and residues in the sequence 306-319 at the cytoplasmic end of helix TM7 and in helix H8
    C Altenbach
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-7008, USA
    Biochemistry 40:15483-92. 2001
  9. ncbi request reprint Estimation of inter-residue distances in spin labeled proteins at physiological temperatures: experimental strategies and practical limitations
    C Altenbach
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-7008, USA
    Biochemistry 40:15471-82. 2001
  10. ncbi request reprint Membrane topology of helices VII and XI in the lactose permease of Escherichia coli studied by lacY-phoA fusion analysis and site-directed spectroscopy
    M L Ujwal
    Howard Hughes Medical Institute, Department of Physiology, Los Angeles, California, USA
    Biochemistry 34:14909-17. 1995

Research Grants

  1. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne L Hubbell; Fiscal Year: 2010
  2. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne Hubbell; Fiscal Year: 2009
  3. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne Hubbell; Fiscal Year: 2007
  4. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne Hubbell; Fiscal Year: 2005
  5. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne Hubbell; Fiscal Year: 2006
  6. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne Hubbell; Fiscal Year: 2005
  7. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne Hubbell; Fiscal Year: 2004
  8. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne Hubbell; Fiscal Year: 2003
  9. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne Hubbell; Fiscal Year: 2002
  10. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne Hubbell; Fiscal Year: 2001

Collaborators

  • Christian Altenbach
  • HOWARD KABACK
  • BEN GLASGOW
  • H Jung
  • TODD YEATES
  • Jianyong Wu
  • CANDICE KLUG
  • Dmitri S Kudryashov
  • PETER QIN
  • O P Ernst
  • J Feigon
  • Richard M Epand
  • Masahiro Kono
  • E Bibi
  • Thomas Risse
  • Adrian Gross
  • Susan M Hanson
  • Ned Van Eps
  • William M Oldham
  • Heidi E Hamm
  • Anita M Preininger
  • Bernhard Knierim
  • Zhefeng Guo
  • Vsevolod V Gurevich
  • Kalman Hideg
  • Kerstin Jacobsen
  • Jennine M Crane
  • Emil Reisler
  • Linda Columbus
  • J Mario Isas
  • Derek J Francis
  • Sergey A Vishnivetskiy
  • Alexander Shvetsov
  • Ana Karin Kusnetzow
  • Zeynep A Oztug Durer
  • Martina Medkova
  • Thue W Schwartz
  • Klaus Peter Hofmann
  • Duilio Cascio
  • Irina Smirnova
  • Martin Phillips
  • John D Stamm
  • Peter A Rubenstein
  • Qi Cai
  • Damon Scoville
  • Linda L Randall
  • Chunfeng Mao
  • Yuying Suo
  • Angela A Lilly
  • Janusz Pyka
  • Michael A Lietzow
  • Jong Myoung Kim
  • Harry T Haigler
  • Jeffrey H Zhang
  • Ralf Langen
  • Nan Jun Yu
  • Alexander Barnakov
  • Ahmed A Serag
  • Mark D Sutton
  • L Columbus
  • J Voss
  • Michael R Sawaya
  • A A Serag
  • Wolfgang Gartner
  • Eric S Dawson
  • Jens Meiler
  • Vladimir Kasho
  • Vadim Y Arshavsky
  • Jun Yong Choe
  • Tamas Kalai
  • Dora Warshaviak
  • Elena A Kolobova
  • Dora Toledo-Warshaviak
  • Eric J Chambers
  • Ian S Haworth
  • Gian Paola C Gacho
  • Virginia F Smith
  • Shirley Oga
  • Jan Ilnicki
  • Wojciech Froncisz
  • Daniel D Oprian
  • K Cai
  • H Gobind Khorana
  • Gaoping Xiao
  • Robert P Gunsalus
  • Issay Narumi
  • Gerald L Hazelbauer
  • Ludmila Barnakova
  • Michael Costanzo
  • Angelina Guzzo

Detail Information

Publications59

  1. pmc Technological advances in site-directed spin labeling of proteins
    Wayne L Hubbell
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, United States Electronic address
    Curr Opin Struct Biol 23:725-33. 2013
    ....
  2. ncbi request reprint Watching proteins move using site-directed spin labeling
    W L Hubbell
    Jules Stein Eye Institute, University of California at Los Angeles 90095 7008, USA
    Structure 4:779-83. 1996
    ..We anticipate future applications to the study of protein folding both in solution and in chaperone-mediated systems...
  3. ncbi request reprint Recent advances in site-directed spin labeling of proteins
    W L Hubbell
    Jules Stein Eye Institute, University of California, Los Angeles 90095, USA
    Curr Opin Struct Biol 8:649-56. 1998
    ..New applications include studies of beta strands, structure mapping using spin-spin interactions, domain motions in soluble proteins and extensive structural analysis of a number of membrane and soluble proteins...
  4. ncbi request reprint Identifying conformational changes with site-directed spin labeling
    W L Hubbell
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, USA
    Nat Struct Biol 7:735-9. 2000
    ..These features make site-directed spin labeling an attractive approach for the study of structure and dynamics in a wide range of systems...
  5. ncbi request reprint Structure and function in rhodopsin: mapping light-dependent changes in distance between residue 316 in helix 8 and residues in the sequence 60-75, covering the cytoplasmic end of helices TM1 and TM2 and their connection loop CL1
    C Altenbach
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-7008, USA
    Biochemistry 40:15493-500. 2001
    ..Photoactivation of rhodopsin shows a pattern of increases in internitroxide distance between the reference, position 316 in H8, and residues in CL1 and TM2 that suggests an outward displacement of TM2 relative to H8 by approximately 3 A...
  6. ncbi request reprint Helix packing in the lactose permease of Escherichia coli: distances between site-directed nitroxides and a lanthanide
    J Voss
    Chemistry Department, University of California, Davis, California 95616 0935, USA
    Biochemistry 40:3184-8. 2001
    ....
  7. ncbi request reprint Molecular motion of spin labeled side chains in alpha-helices: analysis by variation of side chain structure
    L Columbus
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-7008, USA
    Biochemistry 40:3828-46. 2001
    ..The saturated 4-substituted pyrrolidine nitroxides are shown to be a suitable template for novel "functionalized" side chains designed to mimic salient features of the native side chains they replace...
  8. ncbi request reprint Structure and function in rhodopsin: mapping light-dependent changes in distance between residue 65 in helix TM1 and residues in the sequence 306-319 at the cytoplasmic end of helix TM7 and in helix H8
    C Altenbach
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-7008, USA
    Biochemistry 40:15483-92. 2001
    ..The simplest interpretation of the result is a movement of the cytoplasmic portion of TM7 away from TM1 by 2-4 A...
  9. ncbi request reprint Estimation of inter-residue distances in spin labeled proteins at physiological temperatures: experimental strategies and practical limitations
    C Altenbach
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-7008, USA
    Biochemistry 40:15471-82. 2001
    ..The existence of such distance distributions places important constraints on the interpretation of internitroxide distances in terms of protein structure and structural changes...
  10. ncbi request reprint Membrane topology of helices VII and XI in the lactose permease of Escherichia coli studied by lacY-phoA fusion analysis and site-directed spectroscopy
    M L Ujwal
    Howard Hughes Medical Institute, Department of Physiology, Los Angeles, California, USA
    Biochemistry 34:14909-17. 1995
    ....
  11. ncbi request reprint Side chain mobility and ligand interactions of the G strand of tear lipocalins by site-directed spin labeling
    B J Glasgow
    Department of Pathology, University of California at Los Angeles School of Medicine 90095, USA
    Biochemistry 38:13707-16. 1999
    ..However, unlike beta-lactoglobulin, TL binds 16-doxyl stearic acid, suggesting less steric hindrance and greater promiscuity for TL...
  12. ncbi request reprint Identification of a subunit interface in transthyretin amyloid fibrils: evidence for self-assembly from oligomeric building blocks
    A A Serag
    Molecular Biology Institute, School of Medicine, University of California, Los Angeles, California 90095, USA
    Biochemistry 40:9089-96. 2001
    ..The results clarify varied models of amyloidogenesis by demonstrating that transthyretin amyloid fibrils may assemble from oligomeric protein building blocks rather than structurally rearranged monomers...
  13. ncbi request reprint Structure of the KcsA potassium channel from Streptomyces lividans: a site-directed spin labeling study of the second transmembrane segment
    A Gross
    Jules Stein Eye Institute, Department of Chemistry and Biochemistry, University of California, Los Angeles 90095 7008, USA
    Biochemistry 38:10324-35. 1999
    ..These measurements also reveal the presence of a polarity gradient in the phospholipid bilayer...
  14. ncbi request reprint Quantitative analysis of the isolated GAAA tetraloop/receptor interaction in solution: a site-directed spin labeling study
    P Z Qin
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, USA
    Biochemistry 40:6929-36. 2001
    ..The experimental strategy presented here should have broad utility in quantifying weak interactions that would otherwise be undetectable, for both nucleic acids and nucleic acid-protein complexes...
  15. pmc Structure and function in rhodopsin: topology of the C-terminal polypeptide chain in relation to the cytoplasmic loops
    K Cai
    Jules Stein Eye Institute, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 94:14267-72. 1997
    ..These results suggest the proximity of the C-terminal residue C338 to residues located on the outer face of a cytoplasmic helical extension of the F helix with an apparent increase of distance upon photoactivation...
  16. pmc Structural states and dynamics of the D-loop in actin
    Zeynep A Oztug Durer
    Department of Chemistry and Biochemistry, University of California, Los Angeles, California, USA
    Biophys J 103:930-9. 2012
    ....
  17. ncbi request reprint Structure and dynamics of annexin 12 bound to a planar lipid bilayer
    T Risse
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
    Phys Rev Lett 91:188101. 2003
    ..The angular dependent spectra of the two-dimensional microcrystals are shown to provide information on the degree of ordering of spin labels in a alpha-helix and in turn on the orientation of the alpha-helix with respect to the surface...
  18. pmc Structural determinants of nitroxide motion in spin-labeled proteins: solvent-exposed sites in helix B of T4 lysozyme
    Zhefeng Guo
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095 7008, USA
    Protein Sci 17:228-39. 2008
    ..Such interactions appear to be specific to particular rotamers of the R1 side chain...
  19. ncbi request reprint Rhodopsin and 9-demethyl-retinal analog: effect of a partial agonist on displacement of transmembrane helix 6 in class A G protein-coupled receptors
    Bernhard Knierim
    Institut für Medizinische Physik und Biophysik CCM, Charite Universitatsmedizin Berlin, Chariteplatz 1, D 10117 Berlin, Germany
    J Biol Chem 283:4967-74. 2008
    ..When the proton concentration is further increased, a new state arises in 9-dm pigments that is linked to direct proton uptake at the retinal Schiff base. This state apparently has a conformation distinguishable from the active state...
  20. pmc Structural determinants of nitroxide motion in spin-labeled proteins: tertiary contact and solvent-inaccessible sites in helix G of T4 lysozyme
    Zhefeng Guo
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095 7008, USA
    Protein Sci 16:1069-86. 2007
    ..Residue 118 is at a solvent-inaccessible site in the protein core, and the structure of 118R1, the first reported for the R1 side chain at a buried site, reveals how the side chain is accommodated in an overpacked core...
  21. ncbi request reprint Hydrophobic loop dynamics and actin filament stability
    Damon Scoville
    Department of Chemistry and Biochemistry and Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
    Biochemistry 45:13576-84. 2006
    ..Such states may be exploited within a cell by filament-stabilizing and -destabilizing factors...
  22. ncbi request reprint Mechanism of the receptor-catalyzed activation of heterotrimeric G proteins
    William M Oldham
    Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 6600, USA
    Nat Struct Mol Biol 13:772-7. 2006
    ..These data provide the first direct observation of R*-mediated conformational changes in G proteins and define the structural basis for GDP release from G alpha...
  23. pmc Conformational states and dynamics of rhodopsin in micelles and bilayers
    Ana Karin Kusnetzow
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095 7008, USA
    Biochemistry 45:5538-50. 2006
    ..No specific effects of headgroup composition on receptor conformation in lipid bilayers were found...
  24. pmc Determination of the orientation of T4 lysozyme vectorially bound to a planar-supported lipid bilayer using site-directed spin labeling
    Kerstin Jacobsen
    Fritz Haber Institute of Max Planck Society, Department of Chemical Physics, Berlin, Germany
    Biophys J 88:4351-65. 2005
    ....
  25. pmc Structural origins of constitutive activation in rhodopsin: Role of the K296/E113 salt bridge
    Jong Myoung Kim
    Departments of Biology and Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139
    Proc Natl Acad Sci U S A 101:12508-13. 2004
    ....
  26. pmc High-resolution distance mapping in rhodopsin reveals the pattern of helix movement due to activation
    Christian Altenbach
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 105:7439-44. 2008
    ..The positions of nitroxides in other helices at the cytoplasmic surface remained largely unchanged...
  27. ncbi request reprint Motion of spin label side chains in cellular retinol-binding protein: correlation with structure and nearest-neighbor interactions in an antiparallel beta-sheet
    Michael A Lietzow
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095 7008, USA
    Biochemistry 43:3137-51. 2004
    ....
  28. ncbi request reprint Phosphorylation triggers domain separation in the DNA binding response regulator NarL
    Jeffrey H Zhang
    Jules Stein Eye Institute, Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095 1489, USA
    Biochemistry 42:2552-9. 2003
    ..The data also imply that a single conformation of unphosphorylated NarL exists in solution, and there is no detectable equilibrium between the closed and open conformations...
  29. ncbi request reprint Rhodopsin structure, dynamics, and activation: a perspective from crystallography, site-directed spin labeling, sulfhydryl reactivity, and disulfide cross-linking
    Wayne L Hubbell
    Jules Stein Eye Institute, Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, USA
    Adv Protein Chem 63:243-90. 2003
  30. ncbi request reprint A new spin on protein dynamics
    Linda Columbus
    Dept Chemistry and Biochemistry, Jules Stein Eye Institute, University of California Los Angeles, Los Angeles, CA 90095, USA
    Trends Biochem Sci 27:288-95. 2002
    ....
  31. ncbi request reprint Structure and dynamics of a helical hairpin that mediates calcium-dependent membrane binding of annexin B12
    J Mario Isas
    Department of Physiology and Biophysics, University of California, Irvine, 92697, USA
    J Biol Chem 279:32492-8. 2004
    ..This suggests that annexin B12 alters membrane structure in a manner that may be biologically significant...
  32. pmc Structure and function of the visual arrestin oligomer
    Susan M Hanson
    Department of Pharmacology, Vanderbilt University, Nashville, TN 37232, USA
    EMBO J 26:1726-36. 2007
    ..Thus, the tetramer likely serves as a 'storage' form of arrestin, increasing the arrestin-binding capacity of microtubules while readily dissociating to supply active monomer when it is needed to quench rhodopsin signaling...
  33. pmc Mapping allosteric connections from the receptor to the nucleotide-binding pocket of heterotrimeric G proteins
    William M Oldham
    Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, TN 37232 6600, USA
    Proc Natl Acad Sci U S A 104:7927-32. 2007
    ..The data identify a putative pathway of allosteric changes triggered by receptor binding and, together with previously published data, suggest elements of a mechanism for receptor-catalyzed nucleotide exchange...
  34. ncbi request reprint Arrangement of subunits and ordering of beta-strands in an amyloid sheet
    Ahmed A Serag
    Molecular Biology Institute and School of Medicine, University of California, Los Angeles, California 90095, USA
    Nat Struct Biol 9:734-9. 2002
    ....
  35. pmc Sugar binding induces an outward facing conformation of LacY
    Irina Smirnova
    Department of Physiology, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 104:16504-9. 2007
    ..The DEER measurements in conjunction with molecular modeling based on the x-ray structure provide strong support for the alternative access model and reveal a structure for the outward-facing conformer of LacY...
  36. ncbi request reprint Conformational changes in the amino-terminal helix of the G protein alpha(i1) following dissociation from Gbetagamma subunit and activation
    Martina Medkova
    Department of Pharmacology, Vanderbilt University Medical Center, 23rd Avenue South Pierce, 442 Robinson Research Building, Nashville, TN 37232 6600, USA
    Biochemistry 41:9962-72. 2002
    ..These solution studies reveal insights into conformational changes that occur dynamically in solution...
  37. pmc Sequence of late molecular events in the activation of rhodopsin
    Bernhard Knierim
    Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Chariteplatz 1, D 10117 Berlin, Germany
    Proc Natl Acad Sci U S A 104:20290-5. 2007
    ..Collectively, the data suggest the following temporal sequence of events involved in activation: (i) internal Schiff base proton transfer; (ii) TM6 movement; and (iii) proton uptake from solution and binding of transducin...
  38. pmc Opposing effects of inositol hexakisphosphate on rod arrestin and arrestin2 self-association
    Susan M Hanson
    Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Biochemistry 47:1070-5. 2008
    ..In contrast to arrestin2, IP6 inhibits self-association of rod arrestin, indicating that the structure of these two tetramers in solution is likely different...
  39. doi request reprint Membrane activity of an amphiphilic alpha-helical membrane-proximal cytoplasmic domain of the MoMuLV envelope glycoprotein
    Raquel F Epand
    Department of Biochemistry, McMaster University Health Sciences Center, Hamilton, ON L8N 3Z5 Canada
    Exp Mol Pathol 84:9-17. 2008
    ..These finding indicate a possible mechanism by which the membrane-proximal domain in MoMuLV Env may affect the formation of membrane fusion intermediates...
  40. pmc Site-directed spin labeling of a bacterial chemoreceptor reveals a dynamic, loosely packed transmembrane domain
    Alexander Barnakov
    School of Molecular Biosciences, Washington State University, Pullman, Washington 99164 4660, USA
    Protein Sci 11:1472-81. 2002
    ..A dynamic, loosely packed chemoreceptor domain can account for many experimental observations about the transmembrane domains of chemoreceptors...
  41. pmc A model for the solution structure of the rod arrestin tetramer
    Susan M Hanson
    Department of Pharmacology, Vanderbilt University, Nashville, TN 37232, USA
    Structure 16:924-34. 2008
    ..The structure of the solution tetramer explains its inability to bind rhodopsin and paves the way for experimental studies of the physiological role of rod arrestin self-association...
  42. pmc Structural biology: A moving story of receptors
    Thue W Schwartz
    Nature 455:473-4. 2008
  43. ncbi request reprint Structure and dynamics of a helical hairpin and loop region in annexin 12: a site-directed spin labeling study
    J Mario Isas
    Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA
    Biochemistry 41:1464-73. 2002
    ..Thus, in the short helix D, residues are consistently more mobile and accessible throughout the sequence compared to the residues in the longer, less-solvated and more ordered helix E...
  44. ncbi request reprint A model for the structure of the Escherichia coli SOS-regulated UmuD2 protein
    Mark D Sutton
    Biology Department, 68 633, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    DNA Repair (Amst) 1:77-93. 2002
    ..Implications of these structural differences between the UmuD2 and the UmuD2' homodimers with respect to their roles in managing the actions of the umuC-encoded DNA polymerase are discussed...
  45. pmc Structural and dynamical changes in an alpha-subunit of a heterotrimeric G protein along the activation pathway
    Ned Van Eps
    Jules Stein Eye Institute, Departments of Ophthalmology and Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 103:16194-9. 2006
    ..A previously unreported activation-dependent change in alpha4, distant from the interaction surface, supports a role for this helix in effector binding...
  46. ncbi request reprint Mapping backbone dynamics in solution with site-directed spin labeling: GCN4-58 bZip free and bound to DNA
    Linda Columbus
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095 7008, USA
    Biochemistry 43:7273-87. 2004
    ..As anticipated, the backbone motions are damped in the DNA-bound state, although a gradient of motion persists with residues at the DNA-binding site being the most highly ordered, similar to those of helices on globular proteins...
  47. ncbi request reprint The myristoylated amino terminus of Galpha(i)(1) plays a critical role in the structure and function of Galpha(i)(1) subunits in solution
    Anita M Preininger
    Institute for Neuroscience, Department of Molecular Pharmacology and Biological Chemistry, Northwestern University, Chicago, Illinois 60611, USA
    Biochemistry 42:7931-41. 2003
    ..Collectively, the data suggest that myristoylation is an important structural determinant of the amino terminus of Galpha(i) proteins...
  48. pmc Multiquantum EPR spectroscopy of spin-labeled arrestin K267C at 35 GHz
    Candice S Klug
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Biophys J 88:3641-7. 2005
    ..Feasibility has been established for the use of this technique for site-directed spin-labeling studies of biologically relevant samples, particularly the study of protein structure and dynamics...
  49. ncbi request reprint Monitoring RNA base structure and dynamics using site-directed spin labeling
    Peter Z Qin
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, USA
    Biochemistry 42:6772-83. 2003
    ..These results demonstrate the general utility of the 4-thiouridine/methanethiosulfonate coupling method to introduce nitroxide spin labels into RNA and the ability of the resulting label to probe local structure and dynamics...
  50. ncbi request reprint Site-directed spin labeling studies reveal solution conformational changes in a GAAA tetraloop receptor upon Mg(2+)-dependent docking of a GAAA tetraloop
    Peter Z Qin
    Jules Stein Eye Institute, University of California, Los Angeles, CA 90095, USA
    J Mol Biol 351:1-8. 2005
    ..This is compatible with a high specificity of the tetraloop-receptor interaction...
  51. pmc Accessibility of nitroxide side chains: absolute Heisenberg exchange rates from power saturation EPR
    Christian Altenbach
    Jules Stein Eye Institute, University of California, Los Angeles, California 90095 7008, USA
    Biophys J 89:2103-12. 2005
    ..These results provide a useful means for relating crystallographic and site-directed spin labeling data, and hence comparing crystal and solution structures...
  52. pmc Accessibility and dynamics of nitroxide side chains in T4 lysozyme measured by saturation recovery EPR
    Janusz Pyka
    Department of Biophysics, Faculty of Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    Biophys J 89:2059-68. 2005
    ..In one case, multiple conformations detected in the spectrum are not evident in the saturation recovery, suggesting rapid exchange on the timescale of spin-lattice relaxation...
  53. ncbi request reprint Mapping of the docking of SecA onto the chaperone SecB by site-directed spin labeling: insight into the mechanism of ligand transfer during protein export
    Jennine M Crane
    Department of Biochemistry, University of Missouri, Columbia, MO 65211, USA
    J Mol Biol 353:295-307. 2005
    ..This model in combination with previously published biochemical data provides insight into the transfer of the unfolded polypeptide from the chaperone SecB to SecA...
  54. pmc Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin
    Susan M Hanson
    Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Proc Natl Acad Sci U S A 103:4900-5. 2006
    ..This result identifies this loop as a key recognition site in the arrestin-P-Rh* complex and supports the view that flexible sequences are key elements in protein-protein interactions...
  55. ncbi request reprint Details of the partial unfolding of T4 lysozyme on quartz using site-directed spin labeling
    Kerstin Jacobsen
    Fritz Haber Institute of the Max Planck Society, Department of Chemical Physics, Faradayweg 4 6, 14195 Berlin, Germany
    Angew Chem Int Ed Engl 45:3874-7. 2006
  56. ncbi request reprint Conformational dynamics of loop 262-274 in G- and F-actin
    Alexander Shvetsov
    Department of Chemistry and Biochemistry and Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California 90095, USA
    Biochemistry 45:6541-9. 2006
    ..These results show loop 262-274 fluctuations in G- and F-actin and correlate loop dynamics with actin filament formation and stability...
  57. pmc Sites of interaction of a precursor polypeptide on the export chaperone SecB mapped by site-directed spin labeling
    Jennine M Crane
    Department of Biochemistry, University of Missouri, Columbia, MO 65211, USA
    J Mol Biol 363:63-74. 2006
    ..The sites on SecB that interact with the ligand are compared with the previously identified sites that interact with SecA and a model for transfer of the ligand is discussed...
  58. pmc Site-directed spin labeling measurements of nanometer distances in nucleic acids using a sequence-independent nitroxide probe
    Qi Cai
    Department of Chemistry, University of Southern California, Los Angeles, CA 90089 0744, USA
    Nucleic Acids Res 34:4722-30. 2006
    ..Furthermore, distance measurement in a 42 kD DNA was demonstrated. The results establish R5 as a sequence-independent probe for global structural mapping of DNA and DNA-protein complexes...
  59. ncbi request reprint Identification of protein side chains near the membrane-aqueous interface: a site-directed spin labeling study of KcsA
    Adrian Gross
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, USA
    Biochemistry 41:1123-8. 2002
    ..The results indicate that residues near the interface can be identified on the basis of their high rates of collision with the headgroup region of the bilayer...

Research Grants19

  1. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne L Hubbell; Fiscal Year: 2010
    ..Success in this project is anticipated to contribute to this understanding. ..
  2. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne Hubbell; Fiscal Year: 2009
    ..Success in this project is anticipated to contribute to this understanding. ..
  3. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne Hubbell; Fiscal Year: 2007
    ..Success in this project is anticipated to contribute to this understanding. ..
  4. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne Hubbell; Fiscal Year: 2005
    ..Success in this project is anticipated to contribute to this understanding. ..
  5. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne Hubbell; Fiscal Year: 2006
    ..Success in this project is anticipated to contribute to this understanding. ..
  6. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne Hubbell; Fiscal Year: 2005
    ..Success in this project is anticipated to contribute to this understanding. ..
  7. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne Hubbell; Fiscal Year: 2004
    ..Success in this project is anticipated to contribute to this understanding. ..
  8. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne Hubbell; Fiscal Year: 2003
    ..Success in this project is anticipated to contribute to this understanding. ..
  9. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne Hubbell; Fiscal Year: 2002
    ..Success in this project is anticipated to contribute to this understanding. ..
  10. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne Hubbell; Fiscal Year: 2001
    ..Success in this project is anticipated to contribute to this understanding. ..
  11. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne Hubbell; Fiscal Year: 2000
    ..Success in this project is anticipated to contribute to this understanding. ..