W L Hubbell

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi Structure of the KcsA potassium channel from Streptomyces lividans: a site-directed spin labeling study of the second transmembrane segment
    A Gross
    Jules Stein Eye Institute, Department of Chemistry and Biochemistry, University of California, Los Angeles 90095 7008, USA
    Biochemistry 38:10324-35. 1999
  2. ncbi Structure and function in rhodopsin: mapping light-dependent changes in distance between residue 316 in helix 8 and residues in the sequence 60-75, covering the cytoplasmic end of helices TM1 and TM2 and their connection loop CL1
    C Altenbach
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-7008, USA
    Biochemistry 40:15493-500. 2001
  3. ncbi Molecular motion of spin labeled side chains in alpha-helices: analysis by variation of side chain structure
    L Columbus
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-7008, USA
    Biochemistry 40:3828-46. 2001
  4. ncbi Identifying conformational changes with site-directed spin labeling
    W L Hubbell
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, USA
    Nat Struct Biol 7:735-9. 2000
  5. ncbi Watching proteins move using site-directed spin labeling
    W L Hubbell
    Jules Stein Eye Institute, University of California at Los Angeles 90095 7008, USA
    Structure 4:779-83. 1996
  6. ncbi Structure and function in rhodopsin: mapping light-dependent changes in distance between residue 65 in helix TM1 and residues in the sequence 306-319 at the cytoplasmic end of helix TM7 and in helix H8
    C Altenbach
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-7008, USA
    Biochemistry 40:15483-92. 2001
  7. ncbi Helix packing in the lactose permease of Escherichia coli: distances between site-directed nitroxides and a lanthanide
    J Voss
    Chemistry Department, University of California, Davis, California 95616 0935, USA
    Biochemistry 40:3184-8. 2001
  8. ncbi Recent advances in site-directed spin labeling of proteins
    W L Hubbell
    Jules Stein Eye Institute, University of California, Los Angeles 90095, USA
    Curr Opin Struct Biol 8:649-56. 1998
  9. ncbi Estimation of inter-residue distances in spin labeled proteins at physiological temperatures: experimental strategies and practical limitations
    C Altenbach
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-7008, USA
    Biochemistry 40:15471-82. 2001
  10. ncbi Identification of a subunit interface in transthyretin amyloid fibrils: evidence for self-assembly from oligomeric building blocks
    A A Serag
    Molecular Biology Institute, School of Medicine, University of California, Los Angeles, California 90095, USA
    Biochemistry 40:9089-96. 2001

Collaborators

  • H R Kaback
  • S E Butcher
  • J Wu
  • J Feigon
  • C Altenbach
  • K Hideg
  • L Columbus
  • T Risse
  • J Voss
  • P Z Qin
  • J Klein-Seetharaman
  • A A Serag
  • H G Khorana
  • K Cai
  • A Gross
  • J M Isas
  • H T Haigler
  • V Jacques
  • M Gingery
  • K J Oh
  • T O Yeates
  • R J Trabanino
  • C F Meares

Detail Information

Publications12

  1. ncbi Structure of the KcsA potassium channel from Streptomyces lividans: a site-directed spin labeling study of the second transmembrane segment
    A Gross
    Jules Stein Eye Institute, Department of Chemistry and Biochemistry, University of California, Los Angeles 90095 7008, USA
    Biochemistry 38:10324-35. 1999
    ..These measurements also reveal the presence of a polarity gradient in the phospholipid bilayer...
  2. ncbi Structure and function in rhodopsin: mapping light-dependent changes in distance between residue 316 in helix 8 and residues in the sequence 60-75, covering the cytoplasmic end of helices TM1 and TM2 and their connection loop CL1
    C Altenbach
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-7008, USA
    Biochemistry 40:15493-500. 2001
    ..Photoactivation of rhodopsin shows a pattern of increases in internitroxide distance between the reference, position 316 in H8, and residues in CL1 and TM2 that suggests an outward displacement of TM2 relative to H8 by approximately 3 A...
  3. ncbi Molecular motion of spin labeled side chains in alpha-helices: analysis by variation of side chain structure
    L Columbus
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-7008, USA
    Biochemistry 40:3828-46. 2001
    ..The saturated 4-substituted pyrrolidine nitroxides are shown to be a suitable template for novel "functionalized" side chains designed to mimic salient features of the native side chains they replace...
  4. ncbi Identifying conformational changes with site-directed spin labeling
    W L Hubbell
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, USA
    Nat Struct Biol 7:735-9. 2000
    ..These features make site-directed spin labeling an attractive approach for the study of structure and dynamics in a wide range of systems...
  5. ncbi Watching proteins move using site-directed spin labeling
    W L Hubbell
    Jules Stein Eye Institute, University of California at Los Angeles 90095 7008, USA
    Structure 4:779-83. 1996
    ..We anticipate future applications to the study of protein folding both in solution and in chaperone-mediated systems...
  6. ncbi Structure and function in rhodopsin: mapping light-dependent changes in distance between residue 65 in helix TM1 and residues in the sequence 306-319 at the cytoplasmic end of helix TM7 and in helix H8
    C Altenbach
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-7008, USA
    Biochemistry 40:15483-92. 2001
    ..The simplest interpretation of the result is a movement of the cytoplasmic portion of TM7 away from TM1 by 2-4 A...
  7. ncbi Helix packing in the lactose permease of Escherichia coli: distances between site-directed nitroxides and a lanthanide
    J Voss
    Chemistry Department, University of California, Davis, California 95616 0935, USA
    Biochemistry 40:3184-8. 2001
    ....
  8. ncbi Recent advances in site-directed spin labeling of proteins
    W L Hubbell
    Jules Stein Eye Institute, University of California, Los Angeles 90095, USA
    Curr Opin Struct Biol 8:649-56. 1998
    ..New applications include studies of beta strands, structure mapping using spin-spin interactions, domain motions in soluble proteins and extensive structural analysis of a number of membrane and soluble proteins...
  9. ncbi Estimation of inter-residue distances in spin labeled proteins at physiological temperatures: experimental strategies and practical limitations
    C Altenbach
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-7008, USA
    Biochemistry 40:15471-82. 2001
    ..The existence of such distance distributions places important constraints on the interpretation of internitroxide distances in terms of protein structure and structural changes...
  10. ncbi Identification of a subunit interface in transthyretin amyloid fibrils: evidence for self-assembly from oligomeric building blocks
    A A Serag
    Molecular Biology Institute, School of Medicine, University of California, Los Angeles, California 90095, USA
    Biochemistry 40:9089-96. 2001
    ..The results clarify varied models of amyloidogenesis by demonstrating that transthyretin amyloid fibrils may assemble from oligomeric protein building blocks rather than structurally rearranged monomers...
  11. ncbi Quantitative analysis of the isolated GAAA tetraloop/receptor interaction in solution: a site-directed spin labeling study
    P Z Qin
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, USA
    Biochemistry 40:6929-36. 2001
    ..The experimental strategy presented here should have broad utility in quantifying weak interactions that would otherwise be undetectable, for both nucleic acids and nucleic acid-protein complexes...
  12. ncbi Structure and dynamics of annexin 12 bound to a planar lipid bilayer
    T Risse
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
    Phys Rev Lett 91:188101. 2003
    ..The angular dependent spectra of the two-dimensional microcrystals are shown to provide information on the degree of ordering of spin labels in a alpha-helix and in turn on the orientation of the alpha-helix with respect to the surface...