Johnny Huard

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. ncbi Myoblast-mediated gene transfer to the joint
    C S Day
    Department of Orthopaedic Surgery, Children s Hospital of Pittsburgh, PA 15261, USA
    J Orthop Res 15:894-903. 1997
  2. ncbi Gene therapy and tissue engineering for sports medicine
    Johnny Huard
    University of Pittsburgh, Department of Orthopaedic Surgery, Growth and Development Laboratory, 4151 Rangos Research Center, Pittsburgh, PA 15213, USA
    J Gene Med 5:93-108. 2003
  3. ncbi Muscle injuries and repair: current trends in research
    Johnny Huard
    Growth and Development Laboratory, Children's Hospital of Pittsburgh, PA 15213, USA
    J Bone Joint Surg Am 84:822-32. 2002
  4. ncbi Muscle-derived stem cells: potential for muscle regeneration
    Johnny Huard
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, Department of Orthopaedic Surgery, Molecular Genetics and Biochemistry, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Birth Defects Res C Embryo Today 69:230-7. 2003
  5. ncbi Transforming growth factor-beta1 induces the differentiation of myogenic cells into fibrotic cells in injured skeletal muscle: a key event in muscle fibrogenesis
    Yong Li
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213 2583, USA
    Am J Pathol 164:1007-19. 2004
  6. ncbi Differential effect of BMP4 on NIH/3T3 and C2C12 cells: implications for endochondral bone formation
    Guangheng Li
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213 2582, USA
    J Bone Miner Res 20:1611-23. 2005
  7. ncbi Decorin gene transfer promotes muscle cell differentiation and muscle regeneration
    Yong Li
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Mol Ther 15:1616-22. 2007
  8. ncbi Influence of BMPs on the formation of osteoblastic lesions in metastatic prostate cancer
    Brian T Feeley
    Department of Orthopaedic Surgery, David Geffen School of Medicine at UCLA, Los Angeles, California, USA
    J Bone Miner Res 20:2189-99. 2005
  9. ncbi Therapeutic potential of anterior cruciate ligament-derived stem cells for anterior cruciate ligament reconstruction
    Yutaka Mifune
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, PA 15219, USA
    Cell Transplant 21:1651-65. 2012
  10. ncbi The influence of sex on the chondrogenic potential of muscle-derived stem cells: implications for cartilage regeneration and repair
    Tomoyuki Matsumoto
    Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213 2582, USA
    Arthritis Rheum 58:3809-19. 2008

Detail Information

Publications138 found, 100 shown here

  1. ncbi Myoblast-mediated gene transfer to the joint
    C S Day
    Department of Orthopaedic Surgery, Children s Hospital of Pittsburgh, PA 15261, USA
    J Orthop Res 15:894-903. 1997
    ..A stable gene-delivery vehicle to the joint producing proteins that ameliorate these different musculoskeletal conditions may change the clinical implications of these pathologies...
  2. ncbi Gene therapy and tissue engineering for sports medicine
    Johnny Huard
    University of Pittsburgh, Department of Orthopaedic Surgery, Growth and Development Laboratory, 4151 Rangos Research Center, Pittsburgh, PA 15213, USA
    J Gene Med 5:93-108. 2003
    ....
  3. ncbi Muscle injuries and repair: current trends in research
    Johnny Huard
    Growth and Development Laboratory, Children's Hospital of Pittsburgh, PA 15213, USA
    J Bone Joint Surg Am 84:822-32. 2002
  4. ncbi Muscle-derived stem cells: potential for muscle regeneration
    Johnny Huard
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, Department of Orthopaedic Surgery, Molecular Genetics and Biochemistry, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Birth Defects Res C Embryo Today 69:230-7. 2003
    ..We also describe the remarkable ability of stem cells to regenerate skeletal muscle and their potential use to alleviate the muscle weakness associated with DMD...
  5. ncbi Transforming growth factor-beta1 induces the differentiation of myogenic cells into fibrotic cells in injured skeletal muscle: a key event in muscle fibrogenesis
    Yong Li
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213 2583, USA
    Am J Pathol 164:1007-19. 2004
    ....
  6. ncbi Differential effect of BMP4 on NIH/3T3 and C2C12 cells: implications for endochondral bone formation
    Guangheng Li
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213 2582, USA
    J Bone Miner Res 20:1611-23. 2005
    ..These results suggest that endochondral bone formation may involve different cell types, a finding that could have major implications for the tissue engineering of bone and cartilage...
  7. ncbi Decorin gene transfer promotes muscle cell differentiation and muscle regeneration
    Yong Li
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Mol Ther 15:1616-22. 2007
    ..Decorin gene transfer in vivo promoted skeletal muscle regeneration and accelerated muscle healing after injury. These results suggest that decorin not only prevents fibrosis but also improves muscle regeneration and repair...
  8. ncbi Influence of BMPs on the formation of osteoblastic lesions in metastatic prostate cancer
    Brian T Feeley
    Department of Orthopaedic Surgery, David Geffen School of Medicine at UCLA, Los Angeles, California, USA
    J Bone Miner Res 20:2189-99. 2005
    ....
  9. ncbi Therapeutic potential of anterior cruciate ligament-derived stem cells for anterior cruciate ligament reconstruction
    Yutaka Mifune
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, PA 15219, USA
    Cell Transplant 21:1651-65. 2012
    ..We demonstrated that ACL-derived CD34(+) cells contributed to the tendon-bone healing after ACL reconstruction via the enhancement of angiogenesis and osteogenesis, which also contributed to an increase in biomechanical strength...
  10. ncbi The influence of sex on the chondrogenic potential of muscle-derived stem cells: implications for cartilage regeneration and repair
    Tomoyuki Matsumoto
    Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213 2582, USA
    Arthritis Rheum 58:3809-19. 2008
    ..To explore possible differences in muscle-derived stem cell (MDSC) chondrogenic differentiation in vitro and articular cartilage regeneration in vivo between murine male MDSCs (M-MDSCs) and female MDSCs (F-MDSCs)...
  11. ncbi Dystrophin delivery in dystrophin-deficient DMDmdx skeletal muscle by isogenic muscle-derived stem cell transplantation
    Makoto Ikezawa
    Growth and Development Laboratory, Children's Hospital of Pittsburgh, 3460 Fifth Avenue, Pittsburgh, PA 15213, USA
    Hum Gene Ther 14:1535-46. 2003
    ....
  12. ncbi Blocking vascular endothelial growth factor with soluble Flt-1 improves the chondrogenic potential of mouse skeletal muscle-derived stem cells
    Seiji Kubo
    Children s Hospital of Pittsburgh, and University of Pittsburgh, Pittsburgh, Pennsylvania 15213 2582, USA
    Arthritis Rheum 60:155-65. 2009
    ....
  13. ncbi VEGF improves, whereas sFlt1 inhibits, BMP2-induced bone formation and bone healing through modulation of angiogenesis
    Hairong Peng
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Bone Miner Res 20:2017-27. 2005
    ..Results indicate that VEGF antagonist inhibited BMP2-elicited bone formation, whereas the delivery of exogenous VEGF enhanced BMP2-induced bone formation and bone healing through modulation of angiogenesis...
  14. ncbi The synergistic effect of treadmill running on stem-cell transplantation to heal injured skeletal muscle
    Fabrisia Ambrosio
    Department of Physical Medicine and Rehabilitation, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Tissue Eng Part A 16:839-49. 2010
    ..00, p = 0.03, and p = 0.02, respectively). Results suggest that the mechanical stimulation favors MDSC proliferation, both in vitro and in vivo, and that exercise enhances MDSC transplantation after injury...
  15. ncbi The effect of relaxin treatment on skeletal muscle injuries
    Shinichi Negishi
    University of Pittsburgh and Children's Hospital of Pittsburgh, PA 15213, USA
    Am J Sports Med 33:1816-24. 2005
    ..CLINICAL RELEVANCE: These findings may facilitate the development of techniques to eliminate fibrosis, enhance muscle regeneration, and improve functional recovery after muscle injuries...
  16. ncbi Purification and culture of human blood vessel-associated progenitor cells
    Mihaela Crisan
    Children s Hospital of Pittsburgh of UPMC, Pittsburgh, Pennsylvania, USA
    Curr Protoc Stem Cell Biol . 2008
    ..The culture conditions amenable to the long-term proliferation of these blood vessel-associated stem cells in vitro are also described...
  17. ncbi Cartilage repair using bone morphogenetic protein 4 and muscle-derived stem cells
    Ryosuke Kuroda
    Children's Hospital of Pittsburgh and University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Arthritis Rheum 54:433-42. 2006
    ..CONCLUSION: Local delivery of BMP-4 by genetically engineered MDSCs enhanced chondrogenesis and significantly improved articular cartilage repair in rats...
  18. ncbi Human skeletal muscle cells with a slow adhesion rate after isolation and an enhanced stress resistance improve function of ischemic hearts
    Masaho Okada
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Mol Ther 20:138-45. 2012
    ..In conclusion, these results suggest that adult human skeletal muscle-derived cells are inherently heterogeneous with regard to their efficacy for enhancing cardiac function after cardiac implantation, with SACs outperforming RACs...
  19. ncbi Myogenic endothelial cells purified from human skeletal muscle improve cardiac function after transplantation into infarcted myocardium
    Masaho Okada
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Am Coll Cardiol 52:1869-80. 2008
    ..The aim of this study was to evaluate the therapeutic potential of human skeletal muscle-derived myoendothelial cells for myocardial infarct repair...
  20. ncbi NS-398, a cyclooxygenase-2-specific inhibitor, delays skeletal muscle healing by decreasing regeneration and promoting fibrosis
    Wei Shen
    Departments of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Am J Pathol 167:1105-17. 2005
    ..Our findings suggest that the use of cyclooxygenase-2-specific inhibitors to treat skeletal muscle injuries warrants caution because they may interfere with muscle healing...
  21. ncbi Improved muscle healing after contusion injury by the inhibitory effect of suramin on myostatin, a negative regulator of muscle growth
    Masahiro Nozaki
    Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Am J Sports Med 36:2354-62. 2008
    ..Muscle contusions are the most common muscle injuries in sports medicine. Although these injuries are capable of healing, incomplete functional recovery often occurs...
  22. ncbi Noggin improves bone healing elicited by muscle stem cells expressing inducible BMP4
    Hairong Peng
    Growth and Development Laboratory, Children's Hospital of Pittsburgh, Pittsburgh, PA 15213, USA
    Mol Ther 12:239-46. 2005
    ..We believe that this regulatable tissue engineering strategy, enhanced by utilizing a specific antagonist, constitutes a new paradigm for tissue engineering and regenerative medicine...
  23. ncbi Improvement of muscle healing through enhancement of muscle regeneration and prevention of fibrosis
    Kenji Sato
    Growth and Development Laboratory, Department of Orthopaedic Surgery, Children's Hospital, Pittsburgh, Pennsylvania, USA
    Muscle Nerve 28:365-72. 2003
    ..This improvement was not associated with improved responses to physiological testing, at least at the time-points tested in this study...
  24. ncbi Relationships between transforming growth factor-beta1, myostatin, and decorin: implications for skeletal muscle fibrosis
    Jinhong Zhu
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Rangos Research Center, and Department of Bioengineering, University of Pittsburgh, Pennsylvania 15213 2583, USA
    J Biol Chem 282:25852-63. 2007
    ..In summary, these findings define a fibrogenic property of myostatin and suggest the existence of co-regulatory relationships between transforming growth factor-beta1, myostatin, and decorin...
  25. ncbi Structural and functional healing of critical-size segmental bone defects by transduced muscle-derived cells expressing BMP4
    Hsain Chung Shen
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, Pittsburgh, PA 15213 2583, USA
    J Gene Med 6:984-91. 2004
    ..However, we did not evaluate the functionality of the healed bone. The purpose of this study was to determine whether primary muscle-derived cells transduced with retroBMP4 can heal a long bone defect both structurally and functionally...
  26. ncbi Sex of muscle stem cells does not influence potency for cardiac cell therapy
    Lauren Drowley
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA, USA
    Cell Transplant 18:1137-46. 2009
    ..The ability of the MDSCs to improve cardiac regeneration and repair through promotion of angiogenesis without differentiation into the cardiac lineage may have contributed to the lack of sex difference observed in these models...
  27. ncbi Mechanical loading of stem cells for improvement of transplantation outcome in a model of acute myocardial infarction: the role of loading history
    Theresa R Cassino
    Department of Orthopaedic Surgery, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Tissue Eng Part A 18:1101-8. 2012
    ..These findings highlight an important role for cyclic mechanical loading preconditioning of donor MDSCs in optimizing MDSC transplantation for myocardial repair...
  28. ncbi Effect of VEGF on the regenerative capacity of muscle stem cells in dystrophic skeletal muscle
    Bridget M Deasy
    Live Cell Imaging Lab, Children s Hospital of Pittsburgh of UPMC, Pittsburgh, Pennsylvania, USA
    Mol Ther 17:1788-98. 2009
    ..Together the results suggest that the transplantation of VEGF-expressing MDSCs improved skeletal muscle repair through modulation of angiogenesis, regeneration and fibrosis in the injected mdx skeletal muscle...
  29. ncbi Purification and long-term culture of multipotent progenitor cells affiliated with the walls of human blood vessels: myoendothelial cells and pericytes
    Mihaela Crisan
    Department of Pediatrics, Children s Hospital of Pittsburgh of UPMC, Pittsburgh, Pennsylvania 15213, USA
    Methods Cell Biol 86:295-309. 2008
    ..We present in this chapter the main strategies and tactics used to purify, culture on the long term, and phenotypically characterize these novel multipotent cells...
  30. ncbi Timing of the administration of suramin treatment after muscle injury
    Masahiro Nozaki
    Stem Cell Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Muscle Nerve 46:70-9. 2012
    ..The purpose of this study was to determine the optimal timing of suramin administration and investigate the effects it had on the expression of myostatin, follistatin, and muscle vascularity after muscle injury...
  31. ncbi Biological approaches to improve skeletal muscle healing after injury and disease
    Burhan Gharaibeh
    Stem Cell Research Center, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, USA
    Birth Defects Res C Embryo Today 96:82-94. 2012
    ..We believe this medication holds great promise to optimize muscle repair in humans...
  32. ncbi Follistatin improves skeletal muscle healing after injury and disease through an interaction with muscle regeneration, angiogenesis, and fibrosis
    Jinhong Zhu
    Stem Cell Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, USA
    Am J Pathol 179:915-30. 2011
    ..The findings of this study suggest that follistatin is a promising agent for improving skeletal muscle healing after injury and muscle diseases, such as the muscular dystrophies...
  33. ncbi Malignant transformation of multipotent muscle-derived cells by concurrent differentiation signals
    Jonathan B Pollett
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Stem Cells 25:2302-11. 2007
    ..This theory represents a potential link between somatic stem cells and cancer and suggests an involvement of the niche/environment in transformation...
  34. ncbi Improved sphincter contractility after allogenic muscle-derived progenitor cell injection into the denervated rat urethra
    Tracy W Cannon
    Department of Urology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Urology 62:958-63. 2003
    ..MDPC-mediated cellular urethral myoplasty warrants additional investigation as a new method to treat stress urinary incontinence...
  35. ncbi Gamma interferon as an antifibrosis agent in skeletal muscle
    William Foster
    Growth and Development Laboratory, Department of Orthopaedic Surgery, 4151 Rangos Research Center, Children's Hospital of Pittsburgh and University of Pittsburgh, 3705 Fifth Avenue, Pittsburgh, PA 15213-2583, USA
    J Orthop Res 21:798-804. 2003
    ..This study demonstrates that gammaINF is a potent antifibrosis agent that can improve muscle healing after laceration injury...
  36. ncbi Muscle stem cells differentiate into haematopoietic lineages but retain myogenic potential
    Baohong Cao
    Department of Orthopaedic Surgery and Molecular Genetics and Biochemistry, Children's Hospital and University of Pittsburgh, Pittsburgh, PA 15213, USA
    Nat Cell Biol 5:640-6. 2003
    ..These findings demonstrate that MDSC clones retain their myogenic potential after haematopoietic differentiation...
  37. ncbi Antifibrotic effects of suramin in injured skeletal muscle after laceration
    Yi-Sheng Chan
    Department of Orthopaedic Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh, Pittsburgh, PA 15213-2583, USA
    J Appl Physiol 95:771-80. 2003
    ..This study could significantly contribute to the development of strategies to promote efficient muscle healing and functional recovery...
  38. ncbi Ex vivo Noggin gene therapy inhibits bone formation in a mouse model of postoperative resynostosis
    Gregory M Cooper
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA
    Plast Reconstr Surg 123:94S-103S. 2009
    ..This study tested the hypothesis that treatment of suturectomy sites with Noggin, an extracellular antagonist of bone morphogenetic proteins, would inhibit postoperative resynostosis in a mouse suturectomy model...
  39. ncbi Cartilage repair in a rat model of osteoarthritis through intraarticular transplantation of muscle-derived stem cells expressing bone morphogenetic protein 4 and soluble Flt-1
    Tomoyuki Matsumoto
    Stem Cell Research Center, Children s Hospital of Pittsburgh, and University of Pittsburgh, Pittsburgh, Pennsylvania 15213 2582, USA
    Arthritis Rheum 60:1390-405. 2009
    ....
  40. ncbi Differential myocardial infarct repair with muscle stem cells compared to myoblasts
    Hideki Oshima
    Department of Orthopaedic Surgery, University of Pittsburgh, PA 15213-2582, USA
    Mol Ther 12:1130-41. 2005
    ..These findings indicate that MDSCs constitute an alternative to other myogenic cells for use in cardiac repair applications...
  41. ncbi Enhancement of bone healing based on ex vivo gene therapy using human muscle-derived cells expressing bone morphogenetic protein 2
    Joon Yung Lee
    Growth and Development Laboratory, Department of Orthopaedic Surgery, Children's Hospital of Pittsburgh and University of Pittsburgh, Pittsburgh, PA 15260, USA
    Hum Gene Ther 13:1201-11. 2002
    ..These results indicate that genetically engineered human muscle-derived cells enhance bone healing primarily by delivering BMP-2, while a small fraction of the cells seems to differentiate into osteogenic cells...
  42. ncbi Isolation and characterization of human anterior cruciate ligament-derived vascular stem cells
    Tomoyuki Matsumoto
    Stem Cell Research Center, Children s Hospital of Pittsburgh, and Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, USA
    Stem Cells Dev 21:859-72. 2012
    ..In conclusion, our findings suggest that the ACL septum region contains a population of vascular-derived stem cells that may contribute to ligament regeneration and repair at the site of rupture...
  43. ncbi NF-κB negatively impacts the myogenic potential of muscle-derived stem cells
    Aiping Lu
    Stem Cell Research Center, School of Medicine and Department of Orthopedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, USA
    Mol Ther 20:661-8. 2012
    ..Moreover, our results suggest that the improved muscle regeneration observed following inhibition of IKK/NF-κB, is mediated, at least in part, through enhanced stem cell proliferation and myogenic potential...
  44. ncbi The dose of growth factors influences the synergistic effect of vascular endothelial growth factor on bone morphogenetic protein 4-induced ectopic bone formation
    Guangheng Li
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, PA 15213, USA
    Tissue Eng Part A 15:2123-33. 2009
    ..Our studies revealed that VEGF's synergistic role in BMP4 induced ectopic bone formation is dose and cell-type dependent, which is an important consideration for cell-based gene therapy and tissue engineering for bone healing...
  45. ncbi In vivo assessment of a tissue-engineered vascular graft combining a biodegradable elastomeric scaffold and muscle-derived stem cells in a rat model
    Alejandro Nieponice
    Department of Surgery, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA
    Tissue Eng Part A 16:1215-23. 2010
    ..This is the first evidence that MDSCs improve patency and contribute to the remodeling of a tissue-engineered vascular graft for arterial applications...
  46. ncbi Development of a self-inactivating tet-on retroviral vector expressing bone morphogenetic protein 4 to achieve regulated bone formation
    Hairong Peng
    Growth and Development Laboratory, Children's Hospital of Pittsburgh, Pittsburgh, PA 15213, USA
    Mol Ther 9:885-94. 2004
    ....
  47. ncbi A relationship between vascular endothelial growth factor, angiogenesis, and cardiac repair after muscle stem cell transplantation into ischemic hearts
    Thomas R Payne
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania, USA
    J Am Coll Cardiol 50:1677-84. 2007
    ..We investigated whether vascular endothelial growth factor (VEGF) was associated with the angiogenic and therapeutic effects induced after transplantation of skeletal muscle-derived stem cells (MDSCs) into a myocardial infarction (MI)...
  48. ncbi Ex vivo gene transfer to mature skeletal muscle by using adenovirus helper cells
    Shigemi Kimura
    Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA, USA
    J Gene Med 6:155-65. 2004
    ....
  49. ncbi Bone regeneration mediated by BMP4-expressing muscle-derived stem cells is affected by delivery system
    Arvydas Usas
    Stem Cell Research Center, Children s Hospital of Pittsburgh of UPMC and Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Tissue Eng Part A 15:285-93. 2009
    ..In summary, hydrogels are suitable carriers for osteocompetent MDSCs in promoting bone regeneration, especially at craniofacial injury sites...
  50. ncbi BMP4-expressing muscle-derived stem cells differentiate into osteogenic lineage and improve bone healing in immunocompetent mice
    Vonda Wright
    Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA, 15213-2582, USA
    Mol Ther 6:169-78. 2002
    ..Therefore, we believe that technology based on the MDSCs and vector system has great potential for promoting bone healing in a variety of musculoskeletal conditions...
  51. ncbi Differential efficacy of gels derived from small intestinal submucosa as an injectable biomaterial for myocardial infarct repair
    Masaho Okada
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, PA, USA
    Biomaterials 31:7678-83. 2010
    ..Further understanding of SIS gel characteristics, such as biological and physical properties, that are critical determinants of efficacy would be important for optimization of this biomaterial for cardiac repair...
  52. ncbi Nerve growth factor improves the muscle regeneration capacity of muscle stem cells in dystrophic muscle
    Mitra Lavasani
    Department of Bioengineering, University of Pittsburgh, and Growth and Development Laboratory, Children's Hospital of Pittsburgh, Pittsburgh, PA 15213, USA
    Hum Gene Ther 17:180-92. 2006
    ..These findings highlight the importance of NGF as a modulatory molecule, the study of which will broaden our understanding of its biologic role in the regeneration and repair of skeletal muscle by musclederived cells...
  53. ncbi Synergistic enhancement of bone formation and healing by stem cell-expressed VEGF and bone morphogenetic protein-4
    Hairong Peng
    Growth and Development Laboratory, Department of Orthopaedic Surgery, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Clin Invest 110:751-9. 2002
    ..This study has important implications for the formulation of new strategies to improve bone healing through increasing mesenchymal stem cell recruitment and survival, in combination with muscle-derived stem cell-based gene therapy...
  54. ncbi Murine and human myogenic cells identified by elevated aldehyde dehydrogenase activity: implications for muscle regeneration and repair
    Joseph B Vella
    Department of Orthopedic Surgery, Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 6:e29226. 2011
    ..In this study, we hypothesized that elevated ALDH levels could identify murine and human muscle derived cell (hMDC) progenitors, endowed with enhanced stress resistance and muscle regeneration capacity...
  55. ncbi Muscle-derived stem/progenitor cell dysfunction limits healthspan and lifespan in a murine progeria model
    Mitra Lavasani
    Stem Cell Research Center, 206 Bridgeside Point II, 450 Technology Drive, Pittsburgh, Pennsylvania 15219, USA
    Nat Commun 3:608. 2012
    ..These results establish that adult stem/progenitor cell dysfunction contributes to ageing-related degeneration and suggests a therapeutic potential of post-natal stem cells to extend health...
  56. ncbi Osteogenic potential of postnatal skeletal muscle-derived stem cells is influenced by donor sex
    Karin A Corsi
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213 2582, USA
    J Bone Miner Res 22:1592-602. 2007
    ....
  57. ncbi Ex vivo gene therapy-induced endochondral bone formation: comparison of muscle-derived stem cells and different subpopulations of primary muscle-derived cells
    Hsain-Chung Shen
    Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA 15213-2583, USA
    Bone 34:982-92. 2004
    ..The advantage of MDSCs may be attributable, at least in part, to their lower immunogenicity and higher capacity for in vivo survival...
  58. ncbi Mixed metastatic lung cancer lesions in bone are inhibited by noggin overexpression and Rank:Fc administration
    Brian T Feeley
    Department of Orthopaedic Surgery, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    J Bone Miner Res 21:1571-80. 2006
    ..Inhibition of both pathways is necessary to effectively inhibit the progression of mixed metastatic lesions in bone...
  59. ncbi Placental perivascular cells for human muscle regeneration
    Tea Soon Park
    Stem Cell Research Center, Children s Hospital of Pittsburgh of University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
    Stem Cells Dev 20:451-63. 2011
    ....
  60. ncbi Engineering spatial control of multiple differentiation fates within a stem cell population
    Elmer D F Ker
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    Biomaterials 32:3413-22. 2011
    ..This work illustrates spatial control of multi-phenotype differentiation and may have potential in the regeneration of multi-tissue units...
  61. ncbi Perivascular multi-lineage progenitor cells in human organs: regenerative units, cytokine sources or both?
    Chien Wen Chen
    Stem Cell Research Center, Children s Hospital of Pittsburgh and UPMC, Pittsburgh, PA 15213, USA
    Cytokine Growth Factor Rev 20:429-34. 2009
    ..Pericytes can differentiate into diverse cell lineages, but also secrete multiple paracrine growth factors/cytokines, which likely explains in part their robust regenerative potential...
  62. ncbi Prospective identification of myogenic endothelial cells in human skeletal muscle
    Bo Zheng
    Stem Cell Research Center, Children s Hospital of Pittsburgh Department of Orthopaedic Surgery, University of Pittsburgh Children s Hospital and School of Medicine, 4100 Rangos Research Center, 3460 Fifth Avenue, Pittsburgh, PA 15213 2583, USA
    Nat Biotechnol 25:1025-34. 2007
    ..Myoendothelial cells are amenable to biotechnological handling, including purification by flow cytometry and long-term expansion in vitro, and may have potential for the treatment of human muscle disease...
  63. ncbi Retroviral delivery of Noggin inhibits the formation of heterotopic ossification induced by BMP-4, demineralized bone matrix, and trauma in an animal model
    David Hannallah
    Department of Orthopaedic Surgery, University of Pittsburgh, PA 15213-2582, USA
    J Bone Joint Surg Am 86:80-91. 2004
    ..Clinical Relevance: Gene therapy to deliver Noggin may become a powerful method to inhibit heterotopic ossification in targeted areas of the body...
  64. ncbi Converse relationship between in vitro osteogenic differentiation and in vivo bone healing elicited by different populations of muscle-derived cells genetically engineered to express BMP4
    Hairong Peng
    Growth and Development Laboratory, Children's Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Bone Miner Res 19:630-41. 2004
    ....
  65. ncbi Identification and characterization of chondrogenic progenitor cells in the fascia of postnatal skeletal muscle
    Guangheng Li
    Stem Cell Research Center, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA 15219, USA
    J Mol Cell Biol 3:369-77. 2011
    ..Taken together, our results suggest that non-myogenic cells residing in the fascia of skeletal muscle have a strong chondrogenic potential and may represent a novel donor cell source for cartilage regeneration and repair...
  66. ncbi Cellular antioxidant levels influence muscle stem cell therapy
    Lauren Drowley
    Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Mol Ther 18:1865-73. 2010
    ..These results indicate that elevating the levels of antioxidants in MDSCs with NAC can significantly influence their tissue regeneration capacity...
  67. ncbi Cell therapy for muscle regeneration and repair
    Baohong Cao
    Department of Orthopaedic Surgery, University of Pittsburgh, Growth and Development Laboratory, Children's Hospital of Pittsburgh, 4100 Rangos Research Center, 3460 Fifth Avenue, Pittsburgh, PA 15213, USA
    Phys Med Rehabil Clin N Am 16:889-907, viii. 2005
  68. ncbi Inhibited skeletal muscle healing in cyclooxygenase-2 gene-deficient mice: the role of PGE2 and PGF2alpha
    Wei Shen
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, 4100 Rangos Research Center, 3460 Fifth Ave, PA 15213 2583, USA
    J Appl Physiol 101:1215-21. 2006
    ..It suggests that the decision to use NSAIDs to treat muscle injuries warrants critical evaluation because NSAIDs might impair muscle healing by inhibiting the fusion of myogenic precursor cells...
  69. ncbi Interaction between macrophages, TGF-beta1, and the COX-2 pathway during the inflammatory phase of skeletal muscle healing after injury
    Wei Shen
    Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    J Cell Physiol 214:405-12. 2008
    ..Macrophages, TGF-beta1, and the COX-2 pathway products may regulate one another's levels and have profound influence on the whole muscle healing process...
  70. ncbi Pericyte-based human tissue engineered vascular grafts
    Wei He
    Department of Surgery, University of Pittsburgh, USA
    Biomaterials 31:8235-44. 2010
    ..These results demonstrate the feasibility of a pericyte-based TEVG and suggest that the pericytes play a role in maintaining patency of the TEVG as an arterial conduit...
  71. ncbi Muscle-based gene therapy and tissue engineering for treatment of growth plate injuries
    Chang Woo Lee
    Department of Orthopaedic Surgery, Musculoskeletal Research Center, Children's Hospital of Pittsburgh, University of Pittsburgh, Pennsylvania 15213, USA
    J Pediatr Orthop 22:565-72. 2002
    ..Growth plates remained open in groups II and III. This experiment demonstrates that IGF-1 had a supportive effect on physeal chondrocytes, while BMP-2 caused increased osteogenic activity in the injured growth plates...
  72. ncbi Biomechanical characterization of the urethral musculature
    Ron J Jankowski
    Vascular Tissue Engineering Research Laboratories, Rm. 236, Cellomics Bldg, McGowan Institute for Regenerative Medicine, 100 Technology Drive, Pittsburgh, PA 15219, USA
    Am J Physiol Renal Physiol 290:F1127-34. 2006
    ....
  73. ncbi Muscle derived cell mediated ex vivo gene transfer to the lower urinary tract: comparison of viral vectors
    Ryan Pruchnic
    Department of Orthopedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Urol Res 30:310-6. 2002
    ..Our results suggest that the ex vivo approach may lead to an efficient and persistent viral gene delivery to the lower urinary tract while minimizing exposure of the host to virus...
  74. ncbi Biaxial mechanical properties of muscle-derived cell seeded small intestinal submucosa for bladder wall reconstitution
    Shing-Hwa Lu
    Department of Urology, University of Pittsburgh, Pittsburgh, PA 15219, USA
    Biomaterials 26:443-9. 2005
    ..These results suggest that MDC growth was supported by SIS and that initial remodeling of the SIS ECM had occurred within the first 10 days of incubation, but may have slowed once the MDC had grown to confluence within the SIS...
  75. ncbi Microenvironments engineered by inkjet bioprinting spatially direct adult stem cells toward muscle- and bone-like subpopulations
    Julie A Phillippi
    Molecular Biosensor and Imaging Center, Pittsburgh, Pennsylvania, USA
    Stem Cells 26:127-34. 2008
    ..This approach may be useful for understanding cell behaviors to immobilized biological patterns and could have potential applications for regenerative medicine...
  76. ncbi Muscle derived, cell based ex vivo gene therapy for treatment of full thickness articular cartilage defects
    Nobuo Adachi
    Department of Orthopaedic Surgery, Children's Hospital of Pittsburgh, and University of Pittsburgh, Pennsylvania 15213, USA
    J Rheumatol 29:1920-30. 2002
    ..CONCLUSION: Allogeneic MDC could be used for full thickness articular cartilage repair as both a gene delivery vehicle and a cell source for tissue repair...
  77. ncbi The use of suramin, an antifibrotic agent, to improve muscle recovery after strain injury
    Yi-Sheng Chan
    Department of Orthopaedic Surgery, University of Pittsburgh, Pennsylvania 15213, USA
    Am J Sports Med 33:43-51. 2005
    ..Suramin also reduced fibrous scar formation in muscle and enhanced muscle strength in strain-injured skeletal muscle. CLINICAL RELEVANCE: These results may facilitate the development of strategies to enhance muscle healing after injury...
  78. ncbi Isolation of a slowly adhering cell fraction containing stem cells from murine skeletal muscle by the preplate technique
    Burhan Gharaibeh
    Stem Cell Research Center, 4100 Rangos Research Center, 3460 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
    Nat Protoc 3:1501-9. 2008
    ..We also detail additional methodologies designed to further enrich the resultant cell population by continuing the modified preplating process on the SAC population. This process is known as replating and requires further time...
  79. ncbi Differentiation of muscle-derived cells into myofibroblasts in injured skeletal muscle
    Yong Li
    Department of Orthopaedic Surgery, Growth and Development Laboratory, Children's Hospital of Pittsburgh, Pennsylvania 15213-2583, USA
    Am J Pathol 161:895-907. 2002
    ....
  80. ncbi Gene therapy and tissue engineering based on muscle-derived stem cells
    Bridget M Deasy
    Children's Hospital of Pittsburgh, Bioengineering Department, University of Pittsburgh, PA 15213-2582, USA
    Curr Opin Mol Ther 4:382-9. 2002
    ..We will highlight challenges in bridging the gap between understanding basic stem cell biology and clinical utilization for cell therapy...
  81. ncbi Gene therapy and tissue engineering in orthopaedic surgery
    Douglas S Musgrave
    Department of Orthopaedic Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA, USA
    J Am Acad Orthop Surg 10:6-15. 2002
    ..Innovative methodologies that ensure patient safety can potentially lead to many new treatment strategies for musculoskeletal conditions...
  82. ncbi The use of ex vivo gene transfer based on muscle-derived stem cells for cardiovascular medicine
    Tetsuro Sakai
    Department of Surgery, University of Pittsburgh, PA, USA
    Trends Cardiovasc Med 12:115-20. 2002
    ..This review will describe the isolation and unique characteristics of MDSCs and outline their potential use in regenerative medicine...
  83. ncbi Identification of a novel population of muscle stem cells in mice: potential for muscle regeneration
    Zhuqing Qu-Petersen
    Growth and Development Laboratory, Children's Hospital of Pittsburgh, Department of Orthopaedic Surgery, University of Pittsburgh, PA 15260, USA
    J Cell Biol 157:851-64. 2002
    ..Our results suggest that this novel population of muscle-derived stem cells will significantly improve muscle cell-mediated therapies...
  84. ncbi The role of CD34 expression and cellular fusion in the regeneration capacity of myogenic progenitor cells
    Ron J Jankowski
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, 4151 Rangos Research Center, Pittsburgh, PA 15213, USA
    J Cell Sci 115:4361-74. 2002
    ....
  85. ncbi Muscle-derived cell transplantation and differentiation into lower urinary tract smooth muscle
    T Yokoyama
    Department of Urology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Urology 57:826-31. 2001
    ..These results suggest that MDCs can be a desirable substance for tissue engineering and an ex vivo method for gene transfer into the lower urinary tract...
  86. ncbi Extended tropism of an adenoviral vector does not circumvent the maturation-dependent transducibility of mouse skeletal muscle
    J C van Deutekom
    Department of Orthopaedic Surgery, Musculoskeletal Research Center, Children s Hospital of Pittsburgh, PA 15213, USA
    J Gene Med 1:393-9. 1999
    ....
  87. ncbi Cutting-edge muscle recovery: using antifibrosis agents to improve healing
    Yong Li
    Department of Orthopedic Surgery, University of Pittsburgh, Pittsburgh, PA, 15213, USA
    Phys Sportsmed 33:44-50. 2005
    ..Techniques may be refined to prevent muscle fibrosis-specifically via the inactivation of transforming growth factor-beta-1-and, ultimately, improve muscle healing after injuries...
  88. ncbi In vitro interaction between muscle-derived stem cells and nucleus pulposus cells
    Gianluca Vadala
    Ferguson Laboratory for Orthopaedic Research, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Spine J 8:804-9. 2008
    ..The muscle tissue could serve as a good source of adult stem cells because of its vast abundance through out the human body...
  89. ncbi Skeletal muscle fiber type conversion during the repair of mouse soleus: potential implications for muscle healing after injury
    Tetsuya Matsuura
    Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    J Orthop Res 25:1534-40. 2007
    ..These results suggest that PGC-1alpha contributes to myofiber type conversion after muscle injury and that this phenomenon could influence the recovery of the injured muscle...
  90. ncbi Dominant negative Bmp5 mutation reveals key role of BMPs in skeletal response to mechanical stimulation
    Andrew M Ho
    Department of Developmental Biology and Howard Hughes Medical Institute, Beckman Center B300, Stanford University School of Medicine, Stanford, California 94305, USA
    BMC Dev Biol 8:35. 2008
    ....
  91. ncbi Insulin growth factor-1 decreases muscle atrophy following denervation
    Charles S Day
    Department of Orthopaedics, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Microsurgery 22:144-51. 2002
    ..These data suggest that IGF-1 may help prevent muscle atrophy and secondary functional compromise after denervation...
  92. ncbi Effect of cryoinjury on the contractile parameters of bladder strips: a model of impaired detrusor contractility
    George T Somogyi
    Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Brain Res Bull 59:23-8. 2002
    ..The bladder cryoinjury can be used as a model for studying impaired bladder compliance and detrusor contractility as well as treatments that may improve bladder function such as tissue engineering...
  93. ncbi Muscle-derived stem cells
    Baohong Cao
    Departments of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Cell Cycle 3:104-7. 2004
    ..This article summarizes the recent developments in muscle-derived stem cell research...
  94. ncbi Initial failure in myoblast transplantation therapy has led the way toward the isolation of muscle stem cells: potential for tissue regeneration
    Kenneth Urish
    Department of Bioengineering, University of Pittsburgh and Growth and Development Laboratory, Rangos Research Center, Children's Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Curr Top Dev Biol 68:263-80. 2005
    ....
  95. ncbi Inkjet-based biopatterning of bone morphogenetic protein-2 to spatially control calvarial bone formation
    Gregory M Cooper
    Division of Plastic Surgery, Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15224, USA
    Tissue Eng Part A 16:1749-59. 2010
    ..These results demonstrate that three-dimensional biopatterning of a growth factor and growth factor modifier within a construct can direct cell differentiation in vitro and tissue formation in vivo in register to printed patterns...
  96. ncbi Muscle-derived stem cells for tissue engineering and regenerative therapy
    Arvydas Usas
    Stem Cell Research Center, Department of Orthopedic Surgery, Children s Hospital of Pittsburgh, Pittsburgh, PA 15213, USA
    Biomaterials 28:5401-6. 2007
    ..Muscle stem cell-based regenerative therapy and tissue engineering using ex vivo gene therapy, are promising approaches for the treatment of various musculoskeletal, cardiovascular, and urological disorders...
  97. ncbi Stem cells for the treatment of skeletal muscle injury
    Andres J Quintero
    Stem Cell Research Center, Children s Hospital of Pittsburgh, 4100 Rangos Research Center, 3640 Fifth Avenue, Pittsburgh, PA 15213 2582, USA
    Clin Sports Med 28:1-11. 2009
    ....
  98. ncbi Muscle-derived cell-mediated ex vivo gene therapy for urological dysfunction
    J Huard
    Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Gene Ther 9:1617-26. 2002
    ..These results suggest that gene therapy and tissue engineering based on MDC potentially can be used for urological dysfunction...
  99. ncbi Human skeletal muscle cells in ex vivo gene therapy to deliver bone morphogenetic protein-2
    D S Musgrave
    Department of Orthopaedic Surgery, Children s Hospital and the University of Pittsburgh, Pennsylvania 15213 2583, USA
    J Bone Joint Surg Br 84:120-7. 2002
    ....
  100. ncbi The role of receptors in the maturation-dependent adenoviral transduction of myofibers
    B Cao
    Growth and Development Laboratory, Department of Orthopaedic Surgery, Children's Hospital of Pittsburgh and University of Pittsburgh, Pittsburgh, PA, USA
    Gene Ther 8:627-37. 2001
    ..These results taken together suggest that the viral receptor(s) is not a major determinant in maturation-dependent adenoviral transduction of myofibers...
  101. ncbi The effect of bone morphogenetic protein-2 expression on the early fate of skeletal muscle-derived cells
    D S Musgrave
    Growth and Development Laboratory, Department of Orthopedic Surgery, Children's Hospital of Pittsburgh and University of Pittsburgh, 3705 Fifth Avenue, Pittsburgh, PA 15213, USA
    Bone 28:499-506. 2001
    ..These data are important for future investigations into BMP-2 gene delivery approaches to the musculoskeletal system...