Johnny Huard

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. pmc Identification of a novel population of muscle stem cells in mice: potential for muscle regeneration
    Zhuqing Qu-Petersen
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, Department of Orthopaedic Surgery, University of Pittsburgh, PA 15260, USA
    J Cell Biol 157:851-64. 2002
  2. ncbi request reprint Myoblast-mediated gene transfer to the joint
    C S Day
    Department of Orthopaedic Surgery, Children s Hospital of Pittsburgh, PA 15261, USA
    J Orthop Res 15:894-903. 1997
  3. ncbi request reprint Muscle-derived stem cells: potential for muscle regeneration
    Johnny Huard
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, Department of Orthopaedic Surgery, Molecular Genetics and Biochemistry, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Birth Defects Res C Embryo Today 69:230-7. 2003
  4. pmc Transforming growth factor-beta1 induces the differentiation of myogenic cells into fibrotic cells in injured skeletal muscle: a key event in muscle fibrogenesis
    Yong Li
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213 2583, USA
    Am J Pathol 164:1007-19. 2004
  5. ncbi request reprint Muscle injuries and repair: current trends in research
    Johnny Huard
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, PA 15213, USA
    J Bone Joint Surg Am 84:822-32. 2002
  6. ncbi request reprint Gene therapy and tissue engineering for sports medicine
    Johnny Huard
    University of Pittsburgh, Department of Orthopaedic Surgery, Growth and Development Laboratory, 4151 Rangos Research Center, Pittsburgh, PA 15213, USA
    J Gene Med 5:93-108. 2003
  7. ncbi request reprint Differential effect of BMP4 on NIH/3T3 and C2C12 cells: implications for endochondral bone formation
    Guangheng Li
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213 2582, USA
    J Bone Miner Res 20:1611-23. 2005
  8. ncbi request reprint Decorin gene transfer promotes muscle cell differentiation and muscle regeneration
    Yong Li
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Mol Ther 15:1616-22. 2007
  9. ncbi request reprint Influence of BMPs on the formation of osteoblastic lesions in metastatic prostate cancer
    Brian T Feeley
    Department of Orthopaedic Surgery, David Geffen School of Medicine at UCLA, Los Angeles, California, USA
    J Bone Miner Res 20:2189-99. 2005
  10. doi request reprint Therapeutic potential of anterior cruciate ligament-derived stem cells for anterior cruciate ligament reconstruction
    Yutaka Mifune
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, PA 15219, USA
    Cell Transplant 21:1651-65. 2012

Detail Information

Publications148 found, 100 shown here

  1. pmc Identification of a novel population of muscle stem cells in mice: potential for muscle regeneration
    Zhuqing Qu-Petersen
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, Department of Orthopaedic Surgery, University of Pittsburgh, PA 15260, USA
    J Cell Biol 157:851-64. 2002
    ..Our results suggest that this novel population of muscle-derived stem cells will significantly improve muscle cell-mediated therapies...
  2. ncbi request reprint Myoblast-mediated gene transfer to the joint
    C S Day
    Department of Orthopaedic Surgery, Children s Hospital of Pittsburgh, PA 15261, USA
    J Orthop Res 15:894-903. 1997
    ..A stable gene-delivery vehicle to the joint producing proteins that ameliorate these different musculoskeletal conditions may change the clinical implications of these pathologies...
  3. ncbi request reprint Muscle-derived stem cells: potential for muscle regeneration
    Johnny Huard
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, Department of Orthopaedic Surgery, Molecular Genetics and Biochemistry, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Birth Defects Res C Embryo Today 69:230-7. 2003
    ..We also describe the remarkable ability of stem cells to regenerate skeletal muscle and their potential use to alleviate the muscle weakness associated with DMD...
  4. pmc Transforming growth factor-beta1 induces the differentiation of myogenic cells into fibrotic cells in injured skeletal muscle: a key event in muscle fibrogenesis
    Yong Li
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213 2583, USA
    Am J Pathol 164:1007-19. 2004
    ....
  5. ncbi request reprint Muscle injuries and repair: current trends in research
    Johnny Huard
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, PA 15213, USA
    J Bone Joint Surg Am 84:822-32. 2002
  6. ncbi request reprint Gene therapy and tissue engineering for sports medicine
    Johnny Huard
    University of Pittsburgh, Department of Orthopaedic Surgery, Growth and Development Laboratory, 4151 Rangos Research Center, Pittsburgh, PA 15213, USA
    J Gene Med 5:93-108. 2003
    ....
  7. ncbi request reprint Differential effect of BMP4 on NIH/3T3 and C2C12 cells: implications for endochondral bone formation
    Guangheng Li
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213 2582, USA
    J Bone Miner Res 20:1611-23. 2005
    ..These results suggest that endochondral bone formation may involve different cell types, a finding that could have major implications for the tissue engineering of bone and cartilage...
  8. ncbi request reprint Decorin gene transfer promotes muscle cell differentiation and muscle regeneration
    Yong Li
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Mol Ther 15:1616-22. 2007
    ..Decorin gene transfer in vivo promoted skeletal muscle regeneration and accelerated muscle healing after injury. These results suggest that decorin not only prevents fibrosis but also improves muscle regeneration and repair...
  9. ncbi request reprint Influence of BMPs on the formation of osteoblastic lesions in metastatic prostate cancer
    Brian T Feeley
    Department of Orthopaedic Surgery, David Geffen School of Medicine at UCLA, Los Angeles, California, USA
    J Bone Miner Res 20:2189-99. 2005
    ..Our results show that BMPs influence the development and progression of osteoblastic lesions and suggest that therapies that inhibit BMP activity may reduce the formation and progression of osteoblastic lesions...
  10. doi request reprint Therapeutic potential of anterior cruciate ligament-derived stem cells for anterior cruciate ligament reconstruction
    Yutaka Mifune
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, PA 15219, USA
    Cell Transplant 21:1651-65. 2012
    ..We demonstrated that ACL-derived CD34(+) cells contributed to the tendon-bone healing after ACL reconstruction via the enhancement of angiogenesis and osteogenesis, which also contributed to an increase in biomechanical strength...
  11. ncbi request reprint The influence of sex on the chondrogenic potential of muscle-derived stem cells: implications for cartilage regeneration and repair
    Tomoyuki Matsumoto
    Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213 2582, USA
    Arthritis Rheum 58:3809-19. 2008
    ..To explore possible differences in muscle-derived stem cell (MDSC) chondrogenic differentiation in vitro and articular cartilage regeneration in vivo between murine male MDSCs (M-MDSCs) and female MDSCs (F-MDSCs)...
  12. ncbi request reprint VEGF improves, whereas sFlt1 inhibits, BMP2-induced bone formation and bone healing through modulation of angiogenesis
    Hairong Peng
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Bone Miner Res 20:2017-27. 2005
    ..Results indicate that VEGF antagonist inhibited BMP2-elicited bone formation, whereas the delivery of exogenous VEGF enhanced BMP2-induced bone formation and bone healing through modulation of angiogenesis...
  13. pmc Blocking vascular endothelial growth factor with soluble Flt-1 improves the chondrogenic potential of mouse skeletal muscle-derived stem cells
    Seiji Kubo
    Children s Hospital of Pittsburgh, and University of Pittsburgh, Pittsburgh, Pennsylvania 15213 2582, USA
    Arthritis Rheum 60:155-65. 2009
    ....
  14. ncbi request reprint Dystrophin delivery in dystrophin-deficient DMDmdx skeletal muscle by isogenic muscle-derived stem cell transplantation
    Makoto Ikezawa
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, 3460 Fifth Avenue, Pittsburgh, PA 15213, USA
    Hum Gene Ther 14:1535-46. 2003
    ....
  15. pmc The synergistic effect of treadmill running on stem-cell transplantation to heal injured skeletal muscle
    Fabrisia Ambrosio
    Department of Physical Medicine and Rehabilitation, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Tissue Eng Part A 16:839-49. 2010
    ..00, p = 0.03, and p = 0.02, respectively). Results suggest that the mechanical stimulation favors MDSC proliferation, both in vitro and in vivo, and that exercise enhances MDSC transplantation after injury...
  16. ncbi request reprint The effect of relaxin treatment on skeletal muscle injuries
    Shinichi Negishi
    University of Pittsburgh and Children s Hospital of Pittsburgh, PA 15213, USA
    Am J Sports Med 33:1816-24. 2005
    ....
  17. ncbi request reprint Cartilage repair using bone morphogenetic protein 4 and muscle-derived stem cells
    Ryosuke Kuroda
    Children s Hospital of Pittsburgh and University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Arthritis Rheum 54:433-42. 2006
    ....
  18. doi request reprint Purification and culture of human blood vessel-associated progenitor cells
    Mihaela Crisan
    Children s Hospital of Pittsburgh of UPMC, Pittsburgh, Pennsylvania, USA
    Curr Protoc Stem Cell Biol . 2008
    ..The culture conditions amenable to the long-term proliferation of these blood vessel-associated stem cells in vitro are also described...
  19. pmc Human pericytes for ischemic heart repair
    Chien Wen Chen
    Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Stem Cells 31:305-16. 2013
    ..In conclusion, intramyocardial transplantation of purified human pericytes promotes functional and structural recovery, attributable to multiple mechanisms involving paracrine effects and cellular interactions...
  20. pmc Human skeletal muscle cells with a slow adhesion rate after isolation and an enhanced stress resistance improve function of ischemic hearts
    Masaho Okada
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Mol Ther 20:138-45. 2012
    ..In conclusion, these results suggest that adult human skeletal muscle-derived cells are inherently heterogeneous with regard to their efficacy for enhancing cardiac function after cardiac implantation, with SACs outperforming RACs...
  21. pmc NS-398, a cyclooxygenase-2-specific inhibitor, delays skeletal muscle healing by decreasing regeneration and promoting fibrosis
    Wei Shen
    Departments of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Am J Pathol 167:1105-17. 2005
    ..Our findings suggest that the use of cyclooxygenase-2-specific inhibitors to treat skeletal muscle injuries warrants caution because they may interfere with muscle healing...
  22. pmc Myogenic endothelial cells purified from human skeletal muscle improve cardiac function after transplantation into infarcted myocardium
    Masaho Okada
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Am Coll Cardiol 52:1869-80. 2008
    ..The aim of this study was to evaluate the therapeutic potential of human skeletal muscle-derived myoendothelial cells for myocardial infarct repair...
  23. doi request reprint Tendon graft revitalization using adult anterior cruciate ligament (ACL)-derived CD34+ cell sheets for ACL reconstruction
    Yutaka Mifune
    Stem Cell Research Center, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA 15219, USA
    Biomaterials 34:5476-87. 2013
    ..ACL-derived CD34+ cell sheet wrapped grafts could become a promising strategy to revitalize tendon autografts and recovery after ACL reconstruction resulting in superior and quicker recovery in patients requiring ACL reconstruction...
  24. pmc Sustained release of bone morphogenetic protein 2 via coacervate improves the osteogenic potential of muscle-derived stem cells
    Hongshuai Li
    Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA, USA
    Stem Cells Transl Med 2:667-77. 2013
    ..This new strategy represents an alternative approach for bone repair mediated by MDSCs while bypassing the need for gene therapy...
  25. doi request reprint Human myogenic endothelial cells exhibit chondrogenic and osteogenic potentials at the clonal level
    Bo Zheng
    Stem Cell Research Center, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Bridgeside Point II, Suite 206, 450 Technology Drive, Pittsburgh, Pennsylvania 15219, USA
    J Orthop Res 31:1089-95. 2013
    ....
  26. ncbi request reprint Improved muscle healing after contusion injury by the inhibitory effect of suramin on myostatin, a negative regulator of muscle growth
    Masahiro Nozaki
    Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Am J Sports Med 36:2354-62. 2008
    ..Muscle contusions are the most common muscle injuries in sports medicine. Although these injuries are capable of healing, incomplete functional recovery often occurs...
  27. ncbi request reprint Relationships between transforming growth factor-beta1, myostatin, and decorin: implications for skeletal muscle fibrosis
    Jinhong Zhu
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Rangos Research Center, and Department of Bioengineering, University of Pittsburgh, Pennsylvania 15213 2583, USA
    J Biol Chem 282:25852-63. 2007
    ..In summary, these findings define a fibrogenic property of myostatin and suggest the existence of co-regulatory relationships between transforming growth factor-beta1, myostatin, and decorin...
  28. doi request reprint Sex of muscle stem cells does not influence potency for cardiac cell therapy
    Lauren Drowley
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA, USA
    Cell Transplant 18:1137-46. 2009
    ..The ability of the MDSCs to improve cardiac regeneration and repair through promotion of angiogenesis without differentiation into the cardiac lineage may have contributed to the lack of sex difference observed in these models...
  29. ncbi request reprint Improvement of muscle healing through enhancement of muscle regeneration and prevention of fibrosis
    Kenji Sato
    Growth and Development Laboratory, Department of Orthopaedic Surgery, Children s Hospital, Pittsburgh, Pennsylvania, USA
    Muscle Nerve 28:365-72. 2003
    ..This improvement was not associated with improved responses to physiological testing, at least at the time-points tested in this study...
  30. ncbi request reprint Noggin improves bone healing elicited by muscle stem cells expressing inducible BMP4
    Hairong Peng
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, Pittsburgh, PA 15213, USA
    Mol Ther 12:239-46. 2005
    ..We believe that this regulatable tissue engineering strategy, enhanced by utilizing a specific antagonist, constitutes a new paradigm for tissue engineering and regenerative medicine...
  31. ncbi request reprint Structural and functional healing of critical-size segmental bone defects by transduced muscle-derived cells expressing BMP4
    Hsain Chung Shen
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, Pittsburgh, PA 15213 2583, USA
    J Gene Med 6:984-91. 2004
    ..However, we did not evaluate the functionality of the healed bone. The purpose of this study was to determine whether primary muscle-derived cells transduced with retroBMP4 can heal a long bone defect both structurally and functionally...
  32. pmc Mechanical loading of stem cells for improvement of transplantation outcome in a model of acute myocardial infarction: the role of loading history
    Theresa R Cassino
    Department of Orthopaedic Surgery, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Tissue Eng Part A 18:1101-8. 2012
    ..These findings highlight an important role for cyclic mechanical loading preconditioning of donor MDSCs in optimizing MDSC transplantation for myocardial repair...
  33. doi request reprint Purification and long-term culture of multipotent progenitor cells affiliated with the walls of human blood vessels: myoendothelial cells and pericytes
    Mihaela Crisan
    Department of Pediatrics, Children s Hospital of Pittsburgh of UPMC, Pittsburgh, Pennsylvania 15213, USA
    Methods Cell Biol 86:295-309. 2008
    ..We present in this chapter the main strategies and tactics used to purify, culture on the long term, and phenotypically characterize these novel multipotent cells...
  34. pmc Effect of VEGF on the regenerative capacity of muscle stem cells in dystrophic skeletal muscle
    Bridget M Deasy
    Live Cell Imaging Lab, Children s Hospital of Pittsburgh of UPMC, Pittsburgh, Pennsylvania, USA
    Mol Ther 17:1788-98. 2009
    ..Together the results suggest that the transplantation of VEGF-expressing MDSCs improved skeletal muscle repair through modulation of angiogenesis, regeneration and fibrosis in the injected mdx skeletal muscle...
  35. pmc Platelet-rich plasma promotes the proliferation of human muscle derived progenitor cells and maintains their stemness
    Hongshuai Li
    Department of Orthopedic Surgery, Stem Cell Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 8:e64923. 2013
    ..Moreover, PDGF appears to be a key contributing factor to the beneficial effect that PRP has on the proliferation of hMDPCs...
  36. doi request reprint Beneficial effect of mechanical stimulation on the regenerative potential of muscle-derived stem cells is lost by inhibiting vascular endothelial growth factor
    Sarah A Beckman
    Departments of Orthopedic Surgery, University of Pittsburgh, Pittsburgh, PA 15219, USA
    Arterioscler Thromb Vasc Biol 33:2004-12. 2013
    ..The objective of this study was to determine the importance of vascular endothelial growth factor (VEGF) on mechanically stimulated MDSCs in a murine model of muscle regeneration...
  37. doi request reprint The timing of administration of a clinically relevant dose of losartan influences the healing process after contusion induced muscle injury
    Tetsuo Kobayashi
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania, USA
    J Appl Physiol (1985) 114:262-73. 2013
    ..Our study revealed accelerated muscle healing when the 300 mg·kg(-1)·day(-1) of Los was administered immediately after injury and a clinically relevant dose of 10 mg·kg(-1)·day(-1) of Los was administered at 3 or 7 days postinjury...
  38. doi request reprint Timing of the administration of suramin treatment after muscle injury
    Masahiro Nozaki
    Stem Cell Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Muscle Nerve 46:70-9. 2012
    ..The purpose of this study was to determine the optimal timing of suramin administration and investigate the effects it had on the expression of myostatin, follistatin, and muscle vascularity after muscle injury...
  39. doi request reprint Biological approaches to improve skeletal muscle healing after injury and disease
    Burhan Gharaibeh
    Stem Cell Research Center, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, USA
    Birth Defects Res C Embryo Today 96:82-94. 2012
    ..We believe this medication holds great promise to optimize muscle repair in humans...
  40. pmc Follistatin improves skeletal muscle healing after injury and disease through an interaction with muscle regeneration, angiogenesis, and fibrosis
    Jinhong Zhu
    Stem Cell Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, USA
    Am J Pathol 179:915-30. 2011
    ..The findings of this study suggest that follistatin is a promising agent for improving skeletal muscle healing after injury and muscle diseases, such as the muscular dystrophies...
  41. ncbi request reprint Malignant transformation of multipotent muscle-derived cells by concurrent differentiation signals
    Jonathan B Pollett
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Stem Cells 25:2302-11. 2007
    ..This theory represents a potential link between somatic stem cells and cancer and suggests an involvement of the niche/environment in transformation...
  42. ncbi request reprint Differential myocardial infarct repair with muscle stem cells compared to myoblasts
    Hideki Oshima
    Department of Orthopaedic Surgery, University of Pittsburgh, PA 15213 2582, USA
    Mol Ther 12:1130-41. 2005
    ..These findings indicate that MDSCs constitute an alternative to other myogenic cells for use in cardiac repair applications...
  43. doi request reprint Cartilage repair in a rat model of osteoarthritis through intraarticular transplantation of muscle-derived stem cells expressing bone morphogenetic protein 4 and soluble Flt-1
    Tomoyuki Matsumoto
    Stem Cell Research Center, Children s Hospital of Pittsburgh, and University of Pittsburgh, Pittsburgh, Pennsylvania 15213 2582, USA
    Arthritis Rheum 60:1390-405. 2009
    ....
  44. ncbi request reprint Enhancement of bone healing based on ex vivo gene therapy using human muscle-derived cells expressing bone morphogenetic protein 2
    Joon Yung Lee
    Growth and Development Laboratory, Department of Orthopaedic Surgery, Children s Hospital of Pittsburgh and University of Pittsburgh, Pittsburgh, PA 15260, USA
    Hum Gene Ther 13:1201-11. 2002
    ..These results indicate that genetically engineered human muscle-derived cells enhance bone healing primarily by delivering BMP-2, while a small fraction of the cells seems to differentiate into osteogenic cells...
  45. ncbi request reprint Improved sphincter contractility after allogenic muscle-derived progenitor cell injection into the denervated rat urethra
    Tracy W Cannon
    Department of Urology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Urology 62:958-63. 2003
    ..To study the physiologic outcome of allogenic transplant of muscle-derived progenitor cells (MDPCs) in the denervated female rat urethra...
  46. doi request reprint Ex vivo Noggin gene therapy inhibits bone formation in a mouse model of postoperative resynostosis
    Gregory M Cooper
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA
    Plast Reconstr Surg 123:94S-103S. 2009
    ..This study tested the hypothesis that treatment of suturectomy sites with Noggin, an extracellular antagonist of bone morphogenetic proteins, would inhibit postoperative resynostosis in a mouse suturectomy model...
  47. ncbi request reprint Gamma interferon as an antifibrosis agent in skeletal muscle
    William Foster
    Growth and Development Laboratory, Department of Orthopaedic Surgery, 4151 Rangos Research Center, Children s Hospital of Pittsburgh and University of Pittsburgh, 3705 Fifth Avenue, Pittsburgh, PA 15213 2583, USA
    J Orthop Res 21:798-804. 2003
    ..This study demonstrates that gammaINF is a potent antifibrosis agent that can improve muscle healing after laceration injury...
  48. ncbi request reprint Muscle stem cells differentiate into haematopoietic lineages but retain myogenic potential
    Baohong Cao
    Department of Orthopaedic Surgery and Molecular Genetics and Biochemistry, Children s Hospital and University of Pittsburgh, Pittsburgh, PA 15213, USA
    Nat Cell Biol 5:640-6. 2003
    ..These findings demonstrate that MDSC clones retain their myogenic potential after haematopoietic differentiation...
  49. ncbi request reprint Antifibrotic effects of suramin in injured skeletal muscle after laceration
    Yi Sheng Chan
    Department of Orthopaedic Surgery, Children s Hospital of Pittsburgh, University of Pittsburgh, Pittsburgh, PA 15213 2583, USA
    J Appl Physiol 95:771-80. 2003
    ..This study could significantly contribute to the development of strategies to promote efficient muscle healing and functional recovery...
  50. pmc Chick embryo extract demethylates tumor suppressor genes in osteosarcoma cells
    Xiaodong Mu
    Cancer Stem Cell Laboratory, Stem Cell Research Center, Department of Orthopaedic Surgery, University of Pittsburgh, Bridgeside Point 2, Suite 206, 450 Technology Drive, Pittsburgh, PA, 15219, USA
    Clin Orthop Relat Res 472:865-73. 2014
    ..Chick embryo extract (CEE) has been used for the successful expansion of many specific stem cells and has demonstrated the ability to facilitate DNA demethylation...
  51. pmc Muscle-derived stem cell sheets support pump function and prevent cardiac arrhythmias in a model of chronic myocardial infarction
    Naosumi Sekiya
    Stem Cell Research Center, University of Pittsburgh, Pittsburgh, PA, USA
    Mol Ther 21:662-9. 2013
    ....
  52. doi request reprint Isolation of myogenic stem cells from cultures of cryopreserved human skeletal muscle
    Bo Zheng
    Stem Cell Research Center, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine and Children s Hospital of Pittsburgh of UPMC, Pittsburgh, PA, USA
    Cell Transplant 21:1087-93. 2012
    ..We emphasize the potential application of this new approach to extract therapeutic stem cells from human muscle cells cryogenically banked for clinical purposes...
  53. pmc Isolation and characterization of human anterior cruciate ligament-derived vascular stem cells
    Tomoyuki Matsumoto
    Stem Cell Research Center, Children s Hospital of Pittsburgh, and Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, USA
    Stem Cells Dev 21:859-72. 2012
    ..In conclusion, our findings suggest that the ACL septum region contains a population of vascular-derived stem cells that may contribute to ligament regeneration and repair at the site of rupture...
  54. pmc NF-κB negatively impacts the myogenic potential of muscle-derived stem cells
    Aiping Lu
    Stem Cell Research Center, School of Medicine and Department of Orthopedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, USA
    Mol Ther 20:661-8. 2012
    ..Moreover, our results suggest that the improved muscle regeneration observed following inhibition of IKK/NF-κB, is mediated, at least in part, through enhanced stem cell proliferation and myogenic potential...
  55. pmc In vivo assessment of a tissue-engineered vascular graft combining a biodegradable elastomeric scaffold and muscle-derived stem cells in a rat model
    Alejandro Nieponice
    Department of Surgery, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA
    Tissue Eng Part A 16:1215-23. 2010
    ..This is the first evidence that MDSCs improve patency and contribute to the remodeling of a tissue-engineered vascular graft for arterial applications...
  56. pmc The dose of growth factors influences the synergistic effect of vascular endothelial growth factor on bone morphogenetic protein 4-induced ectopic bone formation
    Guangheng Li
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, PA 15213, USA
    Tissue Eng Part A 15:2123-33. 2009
    ..Our studies revealed that VEGF's synergistic role in BMP4 induced ectopic bone formation is dose and cell-type dependent, which is an important consideration for cell-based gene therapy and tissue engineering for bone healing...
  57. ncbi request reprint A relationship between vascular endothelial growth factor, angiogenesis, and cardiac repair after muscle stem cell transplantation into ischemic hearts
    Thomas R Payne
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania, USA
    J Am Coll Cardiol 50:1677-84. 2007
    ..We investigated whether vascular endothelial growth factor (VEGF) was associated with the angiogenic and therapeutic effects induced after transplantation of skeletal muscle-derived stem cells (MDSCs) into a myocardial infarction (MI)...
  58. doi request reprint Differential efficacy of gels derived from small intestinal submucosa as an injectable biomaterial for myocardial infarct repair
    Masaho Okada
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, PA, USA
    Biomaterials 31:7678-83. 2010
    ..Further understanding of SIS gel characteristics, such as biological and physical properties, that are critical determinants of efficacy would be important for optimization of this biomaterial for cardiac repair...
  59. pmc Bone regeneration mediated by BMP4-expressing muscle-derived stem cells is affected by delivery system
    Arvydas Usas
    Stem Cell Research Center, Children s Hospital of Pittsburgh of UPMC and Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Tissue Eng Part A 15:285-93. 2009
    ..In summary, hydrogels are suitable carriers for osteocompetent MDSCs in promoting bone regeneration, especially at craniofacial injury sites...
  60. ncbi request reprint Development of a self-inactivating tet-on retroviral vector expressing bone morphogenetic protein 4 to achieve regulated bone formation
    Hairong Peng
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, Pittsburgh, PA 15213, USA
    Mol Ther 9:885-94. 2004
    ....
  61. ncbi request reprint Nerve growth factor improves the muscle regeneration capacity of muscle stem cells in dystrophic muscle
    Mitra Lavasani
    Department of Bioengineering, University of Pittsburgh, and Growth and Development Laboratory, Children s Hospital of Pittsburgh, Pittsburgh, PA 15213, USA
    Hum Gene Ther 17:180-92. 2006
    ..These findings highlight the importance of NGF as a modulatory molecule, the study of which will broaden our understanding of its biologic role in the regeneration and repair of skeletal muscle by musclederived cells...
  62. pmc Synergistic enhancement of bone formation and healing by stem cell-expressed VEGF and bone morphogenetic protein-4
    Hairong Peng
    Growth and Development Laboratory, Department of Orthopaedic Surgery, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Clin Invest 110:751-9. 2002
    ..This study has important implications for the formulation of new strategies to improve bone healing through increasing mesenchymal stem cell recruitment and survival, in combination with muscle-derived stem cell-based gene therapy...
  63. ncbi request reprint Ex vivo gene transfer to mature skeletal muscle by using adenovirus helper cells
    Shigemi Kimura
    Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA, USA
    J Gene Med 6:155-65. 2004
    ..Here we investigated the use of helper cells to improve the efficiency of ex vivo gene transfer to adult mouse skeletal muscle...
  64. ncbi request reprint BMP4-expressing muscle-derived stem cells differentiate into osteogenic lineage and improve bone healing in immunocompetent mice
    Vonda Wright
    Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA, 15213 2582, USA
    Mol Ther 6:169-78. 2002
    ..Therefore, we believe that technology based on the MDSCs and vector system has great potential for promoting bone healing in a variety of musculoskeletal conditions...
  65. pmc Murine and human myogenic cells identified by elevated aldehyde dehydrogenase activity: implications for muscle regeneration and repair
    Joseph B Vella
    Department of Orthopedic Surgery, Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 6:e29226. 2011
    ..In this study, we hypothesized that elevated ALDH levels could identify murine and human muscle derived cell (hMDC) progenitors, endowed with enhanced stress resistance and muscle regeneration capacity...
  66. pmc Muscle-derived stem/progenitor cell dysfunction limits healthspan and lifespan in a murine progeria model
    Mitra Lavasani
    Stem Cell Research Center, 206 Bridgeside Point II, 450 Technology Drive, Pittsburgh, Pennsylvania 15219, USA
    Nat Commun 3:608. 2012
    ..These results establish that adult stem/progenitor cell dysfunction contributes to ageing-related degeneration and suggests a therapeutic potential of post-natal stem cells to extend health...
  67. doi request reprint Perivascular multi-lineage progenitor cells in human organs: regenerative units, cytokine sources or both?
    Chien Wen Chen
    Stem Cell Research Center, Children s Hospital of Pittsburgh and UPMC, Pittsburgh, PA 15213, USA
    Cytokine Growth Factor Rev 20:429-34. 2009
    ..Pericytes can differentiate into diverse cell lineages, but also secrete multiple paracrine growth factors/cytokines, which likely explains in part their robust regenerative potential...
  68. ncbi request reprint Prospective identification of myogenic endothelial cells in human skeletal muscle
    Bo Zheng
    Stem Cell Research Center, Children s Hospital of Pittsburgh Department of Orthopaedic Surgery, University of Pittsburgh Children s Hospital and School of Medicine, 4100 Rangos Research Center, 3460 Fifth Avenue, Pittsburgh, PA 15213 2583, USA
    Nat Biotechnol 25:1025-34. 2007
    ..Myoendothelial cells are amenable to biotechnological handling, including purification by flow cytometry and long-term expansion in vitro, and may have potential for the treatment of human muscle disease...
  69. ncbi request reprint Osteogenic potential of postnatal skeletal muscle-derived stem cells is influenced by donor sex
    Karin A Corsi
    Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213 2582, USA
    J Bone Miner Res 22:1592-602. 2007
    ....
  70. ncbi request reprint Converse relationship between in vitro osteogenic differentiation and in vivo bone healing elicited by different populations of muscle-derived cells genetically engineered to express BMP4
    Hairong Peng
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Bone Miner Res 19:630-41. 2004
    ..Surprisingly, the use of PP1 cells resulted in an improved outcome because of the lack of adverse responses to BMP4 involving cell differentiation, proliferation, and apoptosis...
  71. ncbi request reprint Mixed metastatic lung cancer lesions in bone are inhibited by noggin overexpression and Rank:Fc administration
    Brian T Feeley
    Department of Orthopaedic Surgery, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    J Bone Miner Res 21:1571-80. 2006
    ..Inhibition of the osteoblastic lesion with noggin and the osteolytic lesion with RANK:Fc was a successful treatment strategy to inhibit progression of mixed lung cancer lesions in bone...
  72. ncbi request reprint Retroviral delivery of Noggin inhibits the formation of heterotopic ossification induced by BMP-4, demineralized bone matrix, and trauma in an animal model
    David Hannallah
    Department of Orthopaedic Surgery, University of Pittsburgh, PA 15213 2582, USA
    J Bone Joint Surg Am 86:80-91. 2004
    ..The heterotopic ossification of muscles, tendons, and ligaments is a common problem faced by orthopaedic surgeons. We investigated the ability of Noggin (a BMP [bone morphogenetic protein] antagonist) to inhibit heterotopic ossification...
  73. ncbi request reprint Ex vivo gene therapy-induced endochondral bone formation: comparison of muscle-derived stem cells and different subpopulations of primary muscle-derived cells
    Hsain Chung Shen
    Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA 15213 2583, USA
    Bone 34:982-92. 2004
    ..The advantage of MDSCs may be attributable, at least in part, to their lower immunogenicity and higher capacity for in vivo survival...
  74. pmc Engineering spatial control of multiple differentiation fates within a stem cell population
    Elmer D F Ker
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    Biomaterials 32:3413-22. 2011
    ..This work illustrates spatial control of multi-phenotype differentiation and may have potential in the regeneration of multi-tissue units...
  75. pmc Placental perivascular cells for human muscle regeneration
    Tea Soon Park
    Stem Cell Research Center, Children s Hospital of Pittsburgh of University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
    Stem Cells Dev 20:451-63. 2011
    ....
  76. pmc Identification and characterization of chondrogenic progenitor cells in the fascia of postnatal skeletal muscle
    Guangheng Li
    Stem Cell Research Center, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA 15219, USA
    J Mol Cell Biol 3:369-77. 2011
    ..Taken together, our results suggest that non-myogenic cells residing in the fascia of skeletal muscle have a strong chondrogenic potential and may represent a novel donor cell source for cartilage regeneration and repair...
  77. ncbi request reprint Muscle-based gene therapy and tissue engineering for treatment of growth plate injuries
    Chang Woo Lee
    Department of Orthopaedic Surgery, Musculoskeletal Research Center, Children s Hospital of Pittsburgh, University of Pittsburgh, Pennsylvania 15213, USA
    J Pediatr Orthop 22:565-72. 2002
    ..Growth plates remained open in groups II and III. This experiment demonstrates that IGF-1 had a supportive effect on physeal chondrocytes, while BMP-2 caused increased osteogenic activity in the injured growth plates...
  78. ncbi request reprint Inhibited skeletal muscle healing in cyclooxygenase-2 gene-deficient mice: the role of PGE2 and PGF2alpha
    Wei Shen
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, 4100 Rangos Research Center, 3460 Fifth Ave, PA 15213 2583, USA
    J Appl Physiol (1985) 101:1215-21. 2006
    ..It suggests that the decision to use NSAIDs to treat muscle injuries warrants critical evaluation because NSAIDs might impair muscle healing by inhibiting the fusion of myogenic precursor cells...
  79. ncbi request reprint Interaction between macrophages, TGF-beta1, and the COX-2 pathway during the inflammatory phase of skeletal muscle healing after injury
    Wei Shen
    Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    J Cell Physiol 214:405-12. 2008
    ..Macrophages, TGF-beta1, and the COX-2 pathway products may regulate one another's levels and have profound influence on the whole muscle healing process...
  80. ncbi request reprint Biomechanical characterization of the urethral musculature
    Ron J Jankowski
    Vascular Tissue Engineering Research Laboratories, Rm 236, Cellomics Bldg, McGowan Institute for Regenerative Medicine, 100 Technology Drive, Pittsburgh, PA 15219, USA
    Am J Physiol Renal Physiol 290:F1127-34. 2006
    ....
  81. ncbi request reprint Muscle derived cell mediated ex vivo gene transfer to the lower urinary tract: comparison of viral vectors
    Ryan Pruchnic
    Department of Orthopedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Urol Res 30:310-6. 2002
    ..Our results suggest that the ex vivo approach may lead to an efficient and persistent viral gene delivery to the lower urinary tract while minimizing exposure of the host to virus...
  82. pmc Cellular antioxidant levels influence muscle stem cell therapy
    Lauren Drowley
    Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Mol Ther 18:1865-73. 2010
    ..These results indicate that elevating the levels of antioxidants in MDSCs with NAC can significantly influence their tissue regeneration capacity...
  83. ncbi request reprint Cell therapy for muscle regeneration and repair
    Baohong Cao
    Department of Orthopaedic Surgery, University of Pittsburgh, Growth and Development Laboratory, Children s Hospital of Pittsburgh, 4100 Rangos Research Center, 3460 Fifth Avenue, Pittsburgh, PA 15213, USA
    Phys Med Rehabil Clin N Am 16:889-907, viii. 2005
  84. pmc Pericyte-based human tissue engineered vascular grafts
    Wei He
    Department of Surgery, University of Pittsburgh, USA
    Biomaterials 31:8235-44. 2010
    ..These results demonstrate the feasibility of a pericyte-based TEVG and suggest that the pericytes play a role in maintaining patency of the TEVG as an arterial conduit...
  85. ncbi request reprint Biaxial mechanical properties of muscle-derived cell seeded small intestinal submucosa for bladder wall reconstitution
    Shing Hwa Lu
    Department of Urology, University of Pittsburgh, Pittsburgh, PA 15219, USA
    Biomaterials 26:443-9. 2005
    ..These results suggest that MDC growth was supported by SIS and that initial remodeling of the SIS ECM had occurred within the first 10 days of incubation, but may have slowed once the MDC had grown to confluence within the SIS...
  86. ncbi request reprint Microenvironments engineered by inkjet bioprinting spatially direct adult stem cells toward muscle- and bone-like subpopulations
    Julie A Phillippi
    Molecular Biosensor and Imaging Center, Pittsburgh, Pennsylvania, USA
    Stem Cells 26:127-34. 2008
    ..This approach may be useful for understanding cell behaviors to immobilized biological patterns and could have potential applications for regenerative medicine...
  87. ncbi request reprint Muscle derived, cell based ex vivo gene therapy for treatment of full thickness articular cartilage defects
    Nobuo Adachi
    Department of Orthopaedic Surgery, Children s Hospital of Pittsburgh, and University of Pittsburgh, Pennsylvania 15213, USA
    J Rheumatol 29:1920-30. 2002
    ....
  88. doi request reprint Isolation of a slowly adhering cell fraction containing stem cells from murine skeletal muscle by the preplate technique
    Burhan Gharaibeh
    Stem Cell Research Center, 4100 Rangos Research Center, 3460 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
    Nat Protoc 3:1501-9. 2008
    ..We also detail additional methodologies designed to further enrich the resultant cell population by continuing the modified preplating process on the SAC population. This process is known as replating and requires further time...
  89. ncbi request reprint The use of suramin, an antifibrotic agent, to improve muscle recovery after strain injury
    Yi Sheng Chan
    Department of Orthopaedic Surgery, University of Pittsburgh, Pennsylvania 15213, USA
    Am J Sports Med 33:43-51. 2005
    ..Muscle strain injuries are extremely common in sports medicine. Muscle healing often is hindered by scar tissue formation after injury...
  90. ncbi request reprint Gene therapy and tissue engineering based on muscle-derived stem cells
    Bridget M Deasy
    Children s Hospital of Pittsburgh, Bioengineering Department, University of Pittsburgh, PA 15213 2582, USA
    Curr Opin Mol Ther 4:382-9. 2002
    ..We will highlight challenges in bridging the gap between understanding basic stem cell biology and clinical utilization for cell therapy...
  91. pmc Differentiation of muscle-derived cells into myofibroblasts in injured skeletal muscle
    Yong Li
    Department of Orthopaedic Surgery, Growth and Development Laboratory, Children s Hospital of Pittsburgh, Pennsylvania 15213 2583, USA
    Am J Pathol 161:895-907. 2002
    ....
  92. ncbi request reprint Gene therapy and tissue engineering in orthopaedic surgery
    Douglas S Musgrave
    Department of Orthopaedic Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA, USA
    J Am Acad Orthop Surg 10:6-15. 2002
    ..Innovative methodologies that ensure patient safety can potentially lead to many new treatment strategies for musculoskeletal conditions...
  93. ncbi request reprint The use of ex vivo gene transfer based on muscle-derived stem cells for cardiovascular medicine
    Tetsuro Sakai
    Department of Surgery, University of Pittsburgh, PA, USA
    Trends Cardiovasc Med 12:115-20. 2002
    ..This review will describe the isolation and unique characteristics of MDSCs and outline their potential use in regenerative medicine...
  94. ncbi request reprint The role of CD34 expression and cellular fusion in the regeneration capacity of myogenic progenitor cells
    Ron J Jankowski
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, 4151 Rangos Research Center, Pittsburgh, PA 15213, USA
    J Cell Sci 115:4361-74. 2002
    ....
  95. ncbi request reprint Muscle-derived cell transplantation and differentiation into lower urinary tract smooth muscle
    T Yokoyama
    Department of Urology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Urology 57:826-31. 2001
    ..These results suggest that MDCs can be a desirable substance for tissue engineering and an ex vivo method for gene transfer into the lower urinary tract...
  96. ncbi request reprint Extended tropism of an adenoviral vector does not circumvent the maturation-dependent transducibility of mouse skeletal muscle
    J C van Deutekom
    Department of Orthopaedic Surgery, Musculoskeletal Research Center, Children s Hospital of Pittsburgh, PA 15213, USA
    J Gene Med 1:393-9. 1999
    ....
  97. ncbi request reprint Effect of cryoinjury on the contractile parameters of bladder strips: a model of impaired detrusor contractility
    George T Somogyi
    Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Brain Res Bull 59:23-8. 2002
    ..The bladder cryoinjury can be used as a model for studying impaired bladder compliance and detrusor contractility as well as treatments that may improve bladder function such as tissue engineering...
  98. ncbi request reprint Muscle-derived stem cells
    Baohong Cao
    Departments of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Cell Cycle 3:104-7. 2004
    ..This article summarizes the recent developments in muscle-derived stem cell research...
  99. pmc Dominant negative Bmp5 mutation reveals key role of BMPs in skeletal response to mechanical stimulation
    Andrew M Ho
    Department of Developmental Biology and Howard Hughes Medical Institute, Beckman Center B300, Stanford University School of Medicine, Stanford, California 94305, USA
    BMC Dev Biol 8:35. 2008
    ....
  100. ncbi request reprint In vitro interaction between muscle-derived stem cells and nucleus pulposus cells
    Gianluca Vadala
    Ferguson Laboratory for Orthopaedic Research, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Spine J 8:804-9. 2008
    ..The muscle tissue could serve as a good source of adult stem cells because of its vast abundance through out the human body...
  101. ncbi request reprint Initial failure in myoblast transplantation therapy has led the way toward the isolation of muscle stem cells: potential for tissue regeneration
    Kenneth Urish
    Department of Bioengineering, University of Pittsburgh and Growth and Development Laboratory, Rangos Research Center, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Curr Top Dev Biol 68:263-80. 2005
    ....