Genomes and Genes
Affiliation: University of California
- Regulated drug bioanalysis for human pharmacokinetic studies and therapeutic drug managementYong Huang
Drug Studies Unit, Department of Bioengineering and Therapeutic Sciences, School of Pharmacy, University of California, San Francisco, CA 94143 0446, USA
Bioanalysis 4:1919-31. 2012..Fostering interaction between bioanalysts from pharmaceutical science and clinical chemistry and reducing the regulatory gaps between different agencies for drug bioanalysis is our objective...
- Matrix effect and recovery terminology issues in regulated drug bioanalysisYong Huang
Drug Studies Unit, Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA 94143, USA
Bioanalysis 4:271-9. 2012..We also provide our personal views on the term 'recovery'...
- Microanalysis of the antiretroviral nevirapine in human hair from HIV-infected patients by liquid chromatography-tandem mass spectrometryYong Huang
Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA 94143, USA
Anal Bioanal Chem 401:1923-33. 2011..We also showed that NVP in human hair can easily be detected in a single short strand of hair. This method will allow us to identify drug non-adherence using even a single strand of hair...
- Sensitive analysis of anti-HIV drugs, efavirenz, lopinavir and ritonavir, in human hair by liquid chromatography coupled with tandem mass spectrometryYong Huang
Department of Biopharmaceutical Sciences, University of California, San Francisco, CA 94143, USA
Rapid Commun Mass Spectrom 22:3401-9. 2008..This developed method allowed for the monitoring of these target medications in the hair samples of HIV-infected women on antiretroviral therapy in an observational study using small amounts of hair...
- A single-nucleotide polymorphism in CYP2B6 leads to >3-fold increases in efavirenz concentrations in plasma and hair among HIV-infected womenMonica Gandhi
Department of Medicine, University of California, San Francisco, CA, USA
J Infect Dis 206:1453-61. 2012..We analyzed numerous genetic and nongenetic factors impacting short-term and long-term exposure in a large heterogeneous population of human immunodeficiency virus (HIV)-infected women...
- Atazanavir concentration in hair is the strongest predictor of outcomes on antiretroviral therapyMonica Gandhi
Departments of Medicine, University of California, San Francisco, 94122, USA
Clin Infect Dis 52:1267-75. 2011..Hair concentrations of antiretrovirals can integrate adherence and pharmacokinetics into a single assay...
- Common clinical conditions - age, low BMI, ritonavir use, mild renal impairment - affect tenofovir pharmacokinetics in a large cohort of HIV-infected womenSanjiv M Baxi
aDepartment of Medicine bDepartment of Clinical Pharmacy cDepartment of Epidemiology and Biostatistics, University of California San Francisco UCSF, San Francisco, California dDepartment of Medicine, SUNY Downstate Medical Center, Brooklyn, New York eDepartment of Bioengineering and Therapeutic Sciences, University of California San Francisco UCSF, San Francisco, California fDepartment of Medicine, Albert Einstein University, Bronx, New York gDepartment of Medicine, Stroger Hospital and Rush University, Chicago, Illinois hDepartment of Medicine, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland iDepartment of Medicine, Georgetown University Medical Center, Washington DC jSection of General Internal Medicine, San Francisco Veterans Affairs Medical Center, San Francisco, California, USA
AIDS 28:59-66. 2014..Tenofovir is used commonly in HIV treatment and prevention settings, but factors that correlate with tenofovir exposure in real-world settings are unknown...
- MicroRNA-23a promotes myelination in the central nervous systemShu Ting Lin
Department of Neurology and Howard Hughes Medical Institute, University of California, San Francisco, CA 94158
Proc Natl Acad Sci U S A 110:17468-73. 2013..The data presented here imply a unique role for miR-23a in the coordination of proteins and noncoding RNAs in generating and maintaining healthy myelin. ..
- Hair and plasma data show that lopinavir, ritonavir, and efavirenz all transfer from mother to infant in utero, but only efavirenz transfers via breastfeedingMonica Gandhi
Department of Medicine, University of California, San Francisco, San Francisco, CA Infectious Diseases Research Collaboration, Makerere University College of Health Sciences University of California, San Francisco, Kampala, Uganda Department of Clinical Pharmacy Center for AIDS Prevention Studies Departments of Pediatrics Bioengineering and Therapeutic Sciences and Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, San Francisco, CA
J Acquir Immune Defic Syndr 63:578-84. 2013..We measured plasma and hair antiretroviral levels in HIV-infected Ugandan mothers and their infants at delivery and during breastfeeding to assess transfer...
- Reversal of the Kynurenine pathway of tryptophan catabolism may improve depression in ART-treated HIV-infected UgandansPriscilla Martinez
Norwegian Center for Addiction Research, University of Oslo, Oslo, Norway Alcohol Research Group, School of Public Health, University of California, Berkeley, CA Center for Global Health, Massachusetts General Hospital, Boston, MA Division of Global Psychiatry, Department of Psychiatry, Massachusetts General Hospital, Boston, MA Mbarara University of Science and Technology, Mbarara, Uganda University of California at San Francisco, San Francisco, CA Division of HIV AIDS, San Francisco General Hospital, University of California San Francisco, CA Department of Global Health and Social Medicine, Harvard Medical School, Boston, MA Department of Global Health and Population, Harvard School of Public Health, Boston, MA and Ragon Institute of Massachusetts General Hospital, MIT and Harvard, Boston, MA
J Acquir Immune Defic Syndr 65:456-62. 2014....
- A simple LC-MS/MS method for determination of kynurenine and tryptophan concentrations in human plasma from HIV-infected patientsYong Huang
Drug Studies Unit, Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA 94143, USA
Bioanalysis 5:1397-407. 2013..In this article, we report a simple, rapid and specific LC-MS/MS method for accurate determination of Kyn and Trp concentrations in human plasma from HIV-infected patients...
- Dysbiosis of the gut microbiota is associated with HIV disease progression and tryptophan catabolismIvan Vujkovic-Cvijin
Division of Experimental Medicine, Department of Medicine, University of California, San Francisco UCSF, San Francisco, CA 94110, USA
Sci Transl Med 5:193ra91. 2013..This link suggests that gut-resident microbial populations may influence intestinal homeostasis during HIV disease. ..
- Preclinical evaluation of HIV eradication strategies in the simian immunodeficiency virus-infected rhesus macaque: a pilot study testing inhibition of indoleamine 2,3-dioxygenaseRichard M Dunham
Department of Medicine, University of California, San Francisco, California 94110, USA
AIDS Res Hum Retroviruses 29:207-14. 2013..In addition, this model might be used to optimize the route and dose by which such interventions are administered and the methods by which their effects are monitored...
- Protease inhibitor levels in hair strongly predict virologic response to treatmentMonica Gandhi
Department of Medicine, University of California, San Francisco, San Francisco, California, USA
AIDS 23:471-8. 2009..The currently available methods to assess ARV exposure are limited. Levels of ARVs in hair reflect plasma concentrations over weeks to months, and may provide a novel method for predicting therapeutic responses...
- Ligand-mediated activation of an engineered gs g protein-coupled receptor in osteoblasts increases trabecular bone formationEdward C Hsiao
Gladstone Institute of Cardiovascular Disease, 1650 Owens Street, San Francisco, California 94158, USA
Mol Endocrinol 24:621-31. 2010..This model provides a powerful tool for investigating the effects of ligand-activated G(s)-GPCR signaling on dynamic bone growth and remodeling...
- 3-Iodothyronamine is an endogenous and rapid-acting derivative of thyroid hormoneThomas S Scanlan
Department of Pharmaceutical Chemistry and Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California 94143 2280, USA
Nat Med 10:638-42. 2004..These results suggest the existence of a new signaling pathway, stimulation of which leads to rapid physiological and behavioral consequences that are opposite those associated with excess TH...
- Casein kinase iδ mutations in familial migraine and advanced sleep phaseK C Brennan
Department of Neurology, University of California, Los Angeles, Los Angeles, CA 90095, USA
Sci Transl Med 5:183ra56, 1-11. 2013..These genetic, cellular, physiological, and behavioral analyses suggest that decreases in CKIδ activity can contribute to the pathogenesis of migraine...
- Strong relationship between oral dose and tenofovir hair levels in a randomized trial: hair as a potential adherence measure for pre-exposure prophylaxis (PrEP)Albert Y Liu
Bridge HIV, San Francisco Department of Public Health, San Francisco, California, United States of America Department of Medicine, University of California San Francisco UCSF, San Francisco, California, United States of America
PLoS ONE 9:e83736. 2014..To evaluate the relationship between dose and tenofovir concentrations in hair, we examined the dose proportionality of tenofovir in hair in healthy, HIV-uninfected adults...
- Short communication: A low-cost method for analyzing nevirapine levels in hair as a marker of adherence in resource-limited settingsMonica Gandhi
1 Division of HIV AIDS, Department of Medicine, University of California, San Francisco, San Francisco, California
AIDS Res Hum Retroviruses 30:25-8. 2014..95-0.996)]. This simple, low-cost method of analyzing nevirapine concentrations in hair may provide a novel monitoring tool for antiretroviral adherence in resource-limited settings and merits further study in clinical settings...
- Higher CD27+CD8+ T cells percentages during suppressive antiretroviral therapy predict greater subsequent CD4+ T cell recovery in treated HIV infectionLillian Seu
Department of Bioengineering and Therapeutic Sciences, Department of Pharmacy, University of California San Francisco, San Francisco, California, United States of America Division of Experimental Medicine, Department of Medicine, San Francisco General Hospital, University of California San Francisco, San Francisco, California, United States of America
PLoS ONE 8:e84091. 2013..092 cells/ul/month, P = 0.028). In HIV-infected subjects starting suppressive ART, patients with the highest percentage of CD8(+) T cells expressing CD27 had the greatest rate of CD4(+) T cell recovery...
- Lamin B1 mediates cell-autonomous neuropathology in a leukodystrophy mouse modelMary Y Heng
Department of Neurology, UCSF, San Francisco, California 94158, USA
J Clin Invest 123:2719-29. 2013..These studies identify a mechanism by which lamin B1 overexpression mediates oligodendrocyte cell-autonomous neuropathology in ADLD and implicate lamin B1 as an important regulator of myelin formation and maintenance during aging...
- Pharmacokinetics of quinacrine efflux from mouse brain via the P-glycoprotein efflux transporterMisol Ahn
Institute for Neurodegenerative Diseases, University of California San Francisco, San Francisco, California, USA
PLoS ONE 7:e39112. 2012..Our findings argue that the inhibition of the P-gp efflux transporter should improve the poor pharmacokinetic properties of quinacrine in the CNS...
- Tryptophan catabolism by indoleamine 2,3-dioxygenase 1 alters the balance of TH17 to regulatory T cells in HIV diseaseDavid Favre
Division of Experimental Medicine, Department of Medicine, University of California, San Francisco, CA 94110, USA
Sci Transl Med 2:32ra36. 2010..We postulate that induction of IDO may represent a critical initiating event that results in inversion of the T(H)17/T(reg) balance and in the consequent maintenance of a chronic inflammatory state in progressive HIV disease...
- Mutations in the gene PRRT2 cause paroxysmal kinesigenic dyskinesia with infantile convulsionsHsien Yang Lee
Department of Neurology, UCSF, San Francisco, CA 94158, USA
Cell Rep 1:2-12. 2012....
- Y-27632 improves rotarod performance and reduces huntingtin levels in R6/2 miceMei Li
Department of Neurology, University of California, San Francisco, CA 94143, USA
Neurobiol Dis 36:413-20. 2009..However, Y-27632 treatment improved rotarod performance significantly, and also reduced soluble brain Htt levels. The ROCK signaling pathway thus remains a promising therapeutic target for HD, and more potent inhibitors may prove useful...
- Distinctive T cell-suppressive signals from nuclearized type 1 sphingosine 1-phosphate G protein-coupled receptorsJia Jun Liao
Department of Medicine, University of California, San Francisco, California 94143, USA
J Biol Chem 282:1964-72. 2007....
- Promotion of lymphocyte egress into blood and lymph by distinct sources of sphingosine-1-phosphateRajita Pappu
Cardiovascular Research Institute, University of California, San Francisco, 600 16th Street S472D, San Francisco, CA 94143 2240, USA
Science 316:295-8. 2007..Disruption of compartmentalized S1P signaling is a plausible mechanism by which S1P-receptor-1 agonists function as immunosuppressives...
- In vitro and in vivo correlation of hepatic transporter effects on erythromycin metabolism: characterizing the importance of transporter-enzyme interplayJustine L Lam
Professor, Department of Biopharmaceutical Sciences, University of California San Francisco, 533 Parnassus, Room U 68, San Francisco, CA 94143 0446, USA
Drug Metab Dispos 34:1336-44. 2006..Hepatic uptake and efflux transporters modulate intracellular concentrations of ERY, thereby affecting metabolism. The interplay of transporters and enzymes must be considered in evaluating potential drug-drug interactions...
- Dose-response effect of tetracyclines on cerebral matrix metalloproteinase-9 after vascular endothelial growth factor hyperstimulationChanhung Z Lee
Department of Anesthesia and Perioperative Care, University of California, San Francisco, 94110, USA
J Cereb Blood Flow Metab 26:1157-64. 2006..This study provided the evidence that the tetracyclines inhibit stimulated cerebral MMP-9 at multiple levels and are effective at very low doses, offering great potential for therapeutic use...
- Lymphocyte sequestration through S1P lyase inhibition and disruption of S1P gradientsSusan R Schwab
Howard Hughes Medical Institute and Department of Microbiology and Immunology, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143 0414, USA
Science 309:1735-9. 2005..We conclude that lymphocyte egress is mediated by S1P gradients that are established by S1P lyase activity and that the lyase may represent a novel immunosuppressant drug target...
- Effects of uremic toxins on hepatic uptake and metabolism of erythromycinHong Sun
Department of Biopharmaceutical Sciences, University of California, San Francisco, California 94143 0446, USA
Drug Metab Dispos 32:1239-46. 2004..Our results suggest that CMPF can directly inhibit the uptake of Ery by inhibiting Oatp2, whereas IS is more likely to inhibit the enzymatic metabolism of Ery...
- Non-competitive androgen receptor inhibition in vitro and in vivoJeremy O Jones
Department of Neurology, University of California San Francisco, San Francisco, CA 94115, USA
Proc Natl Acad Sci U S A 106:7233-8. 2009..Pyrvinium inhibits AR-dependent gene expression in the prostate gland in vivo, and induces prostate atrophy. These results highlight new therapeutic strategies to inhibit AR activity...
- Liquid chromatographic-tandem mass spectrometric assay for the simultaneous determination of didanosine and stavudine in human plasma, bronchoalveolar lavage fluid, alveolar cells, peripheral blood mononuclear cells, seminal plasma, cerebrospinal fluid anYong Huang
Department of Biopharmaceutical Sciences, University of California San Francisco, 94117, San Francisco, CA, USA
J Chromatogr B Analyt Technol Biomed Life Sci 799:51-61. 2004..The lower limits of quantitation for both ddI and d4T were 2.0 ng/ml in plasma; 0.5 ng/ml in CSF; 0.4 ng/ml in AC, PBMC, and BALF; 1.0 ng/ml in seminal plasma; and 0.01 ng/mg in tonsil tissue...