Sheng He Huang

Summary

Affiliation: University of Southern California
Country: USA

Publications

  1. pmc Identification and characterization of an Escherichia coli invasion gene locus, ibeB, required for penetration of brain microvascular endothelial cells
    S H Huang
    Division of Infectious Diseases, Childrens Hospital Los Angeles, Los Angeles, California 90027, USA
    Infect Immun 67:2103-9. 1999
  2. pmc Focal point theory models for dissecting dynamic duality problems of microbial infections
    S H Huang
    Childrens Hospital Los Angeles, University of Southern California, Los Angeles, CA 90027, USA
    J Biomed Biotechnol 2008:856314. 2008
  3. pmc Circulating brain microvascular endothelial cells (cBMECs) as potential biomarkers of the blood-brain barrier disorders caused by microbial and non-microbial factors
    Sheng He Huang
    Department of Pediatrics, Saban Research Institute of Childrens Hospital Los Angeles, University of Southern California, Los Angeles, California, United States of America
    PLoS ONE 8:e62164. 2013
  4. doi request reprint The evolving role of infectomics in drug discovery
    Sheng He Huang
    University of Southern California, Division of Infectious Diseases, Childrens Hospital Los Angeles, Department of Pediatrics, School of Medicine, 4650 Sunset Blvd, Mailstop 51, Los Angeles, CA 90027, USA 1 323 669 4160 1 323 660 2661
    Expert Opin Drug Discov 2:961-75. 2007
  5. ncbi request reprint Infectomics: genomics and proteomics of microbial infections
    Sheng He Huang
    Children s Hospital Los Angeles, University of Southern California School of Medicine, 4650 Sunset Blvd, Los Angeles, CA 90027, USA
    Funct Integr Genomics 1:331-44. 2002
  6. ncbi request reprint A novel genetic island of meningitic Escherichia coli K1 containing the ibeA invasion gene (GimA): functional annotation and carbon-source-regulated invasion of human brain microvascular endothelial cells
    S H Huang
    Department of Pediatrics, Childrens Hospital Los Angeles and the University of Southern California, 4650 Sunset Blvd, Los Angeles, CA 90027, USA
    Funct Integr Genomics 1:312-22. 2001
  7. ncbi request reprint Cellular mechanisms of microbial proteins contributing to invasion of the blood-brain barrier
    S H Huang
    Childrens Hospital Los Angeles and the University of Southern California, Los Angeles, CA 90027, USA
    Cell Microbiol 3:277-87. 2001
  8. ncbi request reprint Further characterization of Escherichia coli brain microvascular endothelial cell invasion gene ibeA by deletion, complementation, and protein expression
    S H Huang
    Childrens Hospital Los Angeles and University of Southern California Keck School of Medicine, Los Angeles, CA 90027, USA
    J Infect Dis 183:1071-8. 2001
  9. ncbi request reprint Enhanced Escherichia coli invasion of human brain microvascular endothelial cells is associated with alternations in cytoskeleton induced by nicotine
    Yu Hua Chen
    Childrens Hospital Los Angeles, University of Southern California, Los Angeles, CA 90027, USA
    Cell Microbiol 4:503-14. 2002
  10. pmc Vimentin and PSF act in concert to regulate IbeA+ E. coli K1 induced activation and nuclear translocation of NF-κB in human brain endothelial cells
    Feng Chi
    Department of Pediatrics, Saban Research Institute of Children s Hospital Los Angeles, University of Southern California, Los Angeles, California, United States of America
    PLoS ONE 7:e35862. 2012

Research Grants

Collaborators

Detail Information

Publications41

  1. pmc Identification and characterization of an Escherichia coli invasion gene locus, ibeB, required for penetration of brain microvascular endothelial cells
    S H Huang
    Division of Infectious Diseases, Childrens Hospital Los Angeles, Los Angeles, California 90027, USA
    Infect Immun 67:2103-9. 1999
    ..coli K1 invasion of BMEC. Taken together, these findings indicate that several E. coli determinants, including ibeA and ibeB, contribute to crossing of the blood-brain barrier...
  2. pmc Focal point theory models for dissecting dynamic duality problems of microbial infections
    S H Huang
    Childrens Hospital Los Angeles, University of Southern California, Los Angeles, CA 90027, USA
    J Biomed Biotechnol 2008:856314. 2008
    ..Symbiotic point (SP), which bears analogy to FP, is a function of SI and PI. SP-converting and specific pathogen-targeting strategies can be used for the rational control of microbial infections...
  3. pmc Circulating brain microvascular endothelial cells (cBMECs) as potential biomarkers of the blood-brain barrier disorders caused by microbial and non-microbial factors
    Sheng He Huang
    Department of Pediatrics, Saban Research Institute of Childrens Hospital Los Angeles, University of Southern California, Los Angeles, California, United States of America
    PLoS ONE 8:e62164. 2013
    ..These results demonstrated that cBMECs as well as EPCs may be used as potential cell-based biomarkers for indexing of BBB injury...
  4. doi request reprint The evolving role of infectomics in drug discovery
    Sheng He Huang
    University of Southern California, Division of Infectious Diseases, Childrens Hospital Los Angeles, Department of Pediatrics, School of Medicine, 4650 Sunset Blvd, Mailstop 51, Los Angeles, CA 90027, USA 1 323 669 4160 1 323 660 2661
    Expert Opin Drug Discov 2:961-75. 2007
    ..Advances in chemical infectomics will lead to the development of a new generation of chemical drugs for therapeutics for microbial infections...
  5. ncbi request reprint Infectomics: genomics and proteomics of microbial infections
    Sheng He Huang
    Children s Hospital Los Angeles, University of Southern California School of Medicine, 4650 Sunset Blvd, Los Angeles, CA 90027, USA
    Funct Integr Genomics 1:331-44. 2002
    ..In this review, we focus on the events that are considered important in infectomics...
  6. ncbi request reprint A novel genetic island of meningitic Escherichia coli K1 containing the ibeA invasion gene (GimA): functional annotation and carbon-source-regulated invasion of human brain microvascular endothelial cells
    S H Huang
    Department of Pediatrics, Childrens Hospital Los Angeles and the University of Southern California, 4650 Sunset Blvd, Los Angeles, CA 90027, USA
    Funct Integr Genomics 1:312-22. 2001
    ..coli K1 invasion gene expression. Our data suggest that this genetic island may contribute to E. coli invasion of the blood-brain barrier through a carbon-source-regulated process...
  7. ncbi request reprint Cellular mechanisms of microbial proteins contributing to invasion of the blood-brain barrier
    S H Huang
    Childrens Hospital Los Angeles and the University of Southern California, Los Angeles, CA 90027, USA
    Cell Microbiol 3:277-87. 2001
    ..Understanding the fundamental mechanisms of microbial penetration of the BBB may help develop novel approaches to prevent the mortality and morbidity associated with central nervous system (CNS) infectious diseases...
  8. ncbi request reprint Further characterization of Escherichia coli brain microvascular endothelial cell invasion gene ibeA by deletion, complementation, and protein expression
    S H Huang
    Childrens Hospital Los Angeles and University of Southern California Keck School of Medicine, Los Angeles, CA 90027, USA
    J Infect Dis 183:1071-8. 2001
    ..coli K1 invasion of BMECs...
  9. ncbi request reprint Enhanced Escherichia coli invasion of human brain microvascular endothelial cells is associated with alternations in cytoskeleton induced by nicotine
    Yu Hua Chen
    Childrens Hospital Los Angeles, University of Southern California, Los Angeles, CA 90027, USA
    Cell Microbiol 4:503-14. 2002
    ..coli-mediated cellular responses. Thus, this study provides experimental evidence for the first time that the major component of tobacco, nicotine, enhances meningitic E. coli invasion of HBMEC through modulation of cytoskeleton...
  10. pmc Vimentin and PSF act in concert to regulate IbeA+ E. coli K1 induced activation and nuclear translocation of NF-κB in human brain endothelial cells
    Feng Chi
    Department of Pediatrics, Saban Research Institute of Children s Hospital Los Angeles, University of Southern California, Los Angeles, California, United States of America
    PLoS ONE 7:e35862. 2012
    ..However, it is unknown how vimentin and PSF cooperatively contribute to IbeA-induced cytoplasmic activation and nuclear translocation of NF-κB, which are required for bacteria-mediated pathogenicities...
  11. doi request reprint Involvement of IbeA in meningitic Escherichia coli K1-induced polymorphonuclear leukocyte transmigration across brain endothelial cells
    Xiaojuan Che
    Saban Research Institute of Childrens Hospital Los Angeles, Department of Pediatrics, University of Southern California, Los Angeles, CA 90027, USA
    Brain Pathol 21:389-404. 2011
    ..Taken together, our data suggested that IbeA and vimentin contribute to E. coli K1-stimulated PMN transendothelial migration that is correlated with upregulation of adhesion molecule expression at the BBB...
  12. ncbi request reprint Cryptococcus neoformans induces alterations in the cytoskeleton of human brain microvascular endothelial cells
    Steven H M Chen
    Division of Hematology Oncology, Children s Hospital Los Angeles, Los Angeles, CA 90027, USA
    J Med Microbiol 52:961-70. 2003
    ..Further characterization of the interactions between C. neoformans and HBMEC should help the development of novel strategies to prevent cryptococcal meningitis and its associated morbidity...
  13. ncbi request reprint PSF is an IbeA-binding protein contributing to meningitic Escherichia coli K1 invasion of human brain microvascular endothelial cells
    Yanming Zou
    Saban Research Institute, Childrens Hospital Los Angeles, Los Angeles, CA 90027, USA
    Med Microbiol Immunol 196:135-43. 2007
    ..coli K1 invasion of HBMEC effectively. Overexpression of PSF in the lentivirus-transducted HBMEC significantly enhanced E. coli K1 invasion. These results suggest that IbeA interacts with PSF for the E. coli K1 invasion of HBMEC...
  14. pmc Cryptococcus neoformans-derived microvesicles enhance the pathogenesis of fungal brain infection
    Sheng He Huang
    Division of Infectious Diseases, The Saban Research Institute, Children s Hospital Los Angeles, Department of Pediatrics, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA
    PLoS ONE 7:e48570. 2012
    ..neoformans-infected brains. Results of this study suggested that CnMVs play an important role in the pathogenesis of cryptococcal meningoencephalitis...
  15. pmc Invasion of Cryptococcus neoformans into human brain microvascular endothelial cells is mediated through the lipid rafts-endocytic pathway via the dual specificity tyrosine phosphorylation-regulated kinase 3 (DYRK3)
    Sheng He Huang
    Department of Pediatrics, Saban Research Institute, Children s Hospital Los Angeles, Los Angeles, California 90027, USA
    J Biol Chem 286:34761-9. 2011
    ..Our data demonstrate that the lipid raft-dependent endocytosis process mediates C. neoformans internalization into HBMEC and that the CD44 protein of the hosts, cytoskeleton, and intracellular kinase-DYRK3 are involved in this process...
  16. pmc Meningitic Escherichia coli K1 penetration and neutrophil transmigration across the blood-brain barrier are modulated by alpha7 nicotinic receptor
    Feng Chi
    Department of Pediatrics, Saban Research Institute, University of Southern California, Childrens Hospital Los Angeles, Los Angeles, California, United States of America
    PLoS ONE 6:e25016. 2011
    ..Taken together, our data suggest that α7 nAChR plays a detrimental role in the host defense against meningitic infection by modulation of pathogen invasion, PMN recruitment, calcium signaling and neuronal inflammation...
  17. doi request reprint Involvement of human CD44 during Cryptococcus neoformans infection of brain microvascular endothelial cells
    Ambrose Jong
    Division of Hematology Oncology, The Saban Research Institute, Childrens Hospital Los Angeles, Department of Pediatrics, Keck School of Medicine, University of Southern California, Los Angeles, CA 90027, USA
    Cell Microbiol 10:1313-26. 2008
    ..Our results highlight the interactions between C. neoformans hyaluronic acid and host CD44 and the dynamic results of these interactions, which may represent events during the adhesion and entry of C. neoformans at HBMEC membrane rafts...
  18. ncbi request reprint Downregulation of human Cdc6 protein using a lentivirus RNA interference expression vector
    Feng Luo
    Division of Hematology Oncology, University of Southern California and Children s Hospital, Los Angeles, CA, USA
    Methods Mol Biol 342:287-93. 2006
    ..Cellular ribonucleases process the shRNA into a functional short interfering RNA (siRNA). Down-regulation of Cdc6 protein is confirmed by Western blots...
  19. doi request reprint Vimentin-mediated signalling is required for IbeA+ E. coli K1 invasion of human brain microvascular endothelial cells
    Feng Chi
    Saban Research Institute of Childrens Hospital Los Angeles, Department of Pediatrics, University of Southern California, Los Angeles, CA 90027, USA
    Biochem J 427:79-90. 2010
    ..coli K1 invasion...
  20. doi request reprint HIV-1 gp41 ectodomain enhances Cryptococcus neoformans binding to human brain microvascular endothelial cells via gp41 core-induced membrane activities
    Sheng He Huang
    Division of Infectious Diseases, Saban Research Institute, Childrens Hospital Los Angeles, University of Southern California, Keck School of Medicine, Los Angeles, CA 90027, USA
    Biochem J 438:457-66. 2011
    ..neoformans binding to HBMECs via gp41 core domain-induced membrane activities, revealing a potential mechanism of invasion for this pathogenic fungus into the brain tissues of HIV-1-infected patients...
  21. ncbi request reprint Involvement of Escherichia coli K1 ibeT in bacterial adhesion that is associated with the entry into human brain microvascular endothelial cells
    Yanming Zou
    Saban Research Institute, Childrens Hospital Los Angeles, Los Angeles, CA, 90027, USA
    Med Microbiol Immunol 197:337-44. 2008
    ..coli E44 in vivo. These data suggest that ibeT-mediated E. coli K1 adhesion is associated with the bacterial invasion process...
  22. doi request reprint Recruitment of α7 nicotinic acetylcholine receptor to caveolin-1-enriched lipid rafts is required for nicotine-enhanced Escherichia coli K1 entry into brain endothelial cells
    Feng Chi
    Saban Research Institute of Childrens Hospital Los Angeles, Department of Pediatrics, University of Southern California, USA
    Future Microbiol 6:953-66. 2011
    ..Conclusion: α7 nAChR-mediated signaling through lipid rafts/caveolae is required for nicotine-enhanced E. coli K1 invasion of HBMEC...
  23. ncbi request reprint Identification of a surface protein on human brain microvascular endothelial cells as vimentin interacting with Escherichia coli invasion protein IbeA
    Yanming Zou
    The Saban Research Institute, Children s Hospital Los Angeles, University of Southern California, CA 90027, USA
    Biochem Biophys Res Commun 351:625-30. 2006
    ..The regulatory protease factor Xa is able to cleave IbeA between R297 and K298 residues, and this cleavage abolishes the IbeA-vimentin interaction...
  24. pmc Hyaluronic acid receptor CD44 deficiency is associated with decreased Cryptococcus neoformans brain infection
    Ambrose Jong
    Division of Hematology Oncology, The Saban Research Institute, Children s Hospital Los Angeles, Los Angeles, California 90027, USA
    J Biol Chem 287:15298-306. 2012
    ..These results suggest that the CD44 and RHAMM receptors function on membrane lipid rafts during invasion and that simvastatin may have a potential therapeutic role in C. neoformans infections of the brain...
  25. pmc Identification and characterization of CPS1 as a hyaluronic acid synthase contributing to the pathogenesis of Cryptococcus neoformans infection
    Ambrose Jong
    Division of Hematology Oncology, Children s Hospital Los Angeles, Los Angeles, CA 90027, USA
    Eukaryot Cell 6:1486-96. 2007
    ..Together, our results support that C. neoformans CPS1 encodes hyaluronic acid synthase and that its product, hyaluronic acid, plays a role as an adhesion molecule during the association of endothelial cells with yeast...
  26. pmc Infectomic analysis of gene expression profiles of human brain microvascular endothelial cells infected with Cryptococcus neoformans
    Ambrose Jong
    Divisions of Hematology Oncology and Infectious Diseases, Saban Research Institute of Childrens Hospital Los Angeles, Keck School of Medicine, University of Southern California, Los Angeles, CA 90027, USA
    J Biomed Biotechnol 2008:375620. 2008
    ..Taken together, our infectomic approaches suggest that the host cells significantly change the gene profiles and also actively participate in immunoregulations of the central nervous system (CNS) during C. neoformans infection...
  27. pmc Cloning and expression of the Escherichia coli K1 outer membrane protein A receptor, a gp96 homologue
    Nemani V Prasadarao
    Division of Infectious Diseases, MS 51, Children s Hospital Los Angeles and Keck School of Medicine, University of Southern California, 4650 Sunset Boulevard, Los Angeles, CA 90027, USA
    Infect Immun 71:1680-8. 2003
    ..Recombinant His-tagged Ecgp blocked E. coli invasion efficiently by binding directly to the bacteria. These results suggest that OmpA of E. coli K1 interacts with a gp96-like molecule on HBMECs for invasion...
  28. ncbi request reprint Gene cloning and expression profiling by rapid amplification of gene inserts with universal vector primers
    Sheng He Huang
    Department of Pediatrics, University of Southern California, Los Angeles, CA, USA
    Methods Mol Biol 192:309-14. 2002
  29. pmc Identification of IbeR as a stationary-phase regulator in meningitic Escherichia coli K1 that carries a loss-of-function mutation in rpoS
    Feng Chi
    Saban Research Institute of Childrens Hospital Los Angeles, Department of Pediatrics, University of Southern California, Los Angeles, CA 90027, USA
    J Biomed Biotechnol 2009:520283. 2009
    ..Survival studies under different stress conditions indicated that IbeR contributed to bacteria stress resistance in the SP. Taken together, IbeR is a novel regulator contributing to the SP regulation...
  30. ncbi request reprint HIV-1 gp41 ectodomain enhances Cryptococcus neoformans binding to HBMEC
    Ambrose Y Jong
    Division of Hematology Oncology, Childrens Hospital Los Angeles, Los Angeles, CA 90027, USA, and Department of Life Science, Catholic Fu Jen University, Taiwan, ROC
    Biochem Biophys Res Commun 356:899-905. 2007
    ..Our results suggest that HIV-1 gp41 ectodomain and C. neoformans may follow a similar invasion mechanism, possibly actin reorganization and/or membrane activation, during pathogen infections on HBMEC...
  31. ncbi request reprint Inverse PCR. Genomic DNA cloning
    Ambrose Y Jong
    Department of Pediatrics, University of Southern California, Los Angeles, CA, USA
    Methods Mol Biol 192:301-7. 2002
  32. pmc Invasion of Cryptococcus neoformans into human brain microvascular endothelial cells requires protein kinase C-alpha activation
    Ambrose Jong
    Division of Hematology Oncology, Children s Hospital Los Angeles, Los Angeles, CA 90027, USA
    Cell Microbiol 10:1854-65. 2008
    ..neoformans invasion. These results suggest a significant role of PKCalpha and downstream actin filament activity during the fungal invasion into HBMEC...
  33. ncbi request reprint Inverse PCR. cDNA cloning
    Sheng He Huang
    Department of Pediatrics, University of Southern California, Los Angeles, CA, USA
    Methods Mol Biol 192:293-9. 2002
  34. ncbi request reprint Use of inverse PCR to clone cDNA ends
    Sheng He Huang
    Department of Pediatrics, University of Southern California, Childrens Hospital, Los Angeles, USA
    Methods Mol Biol 221:51-8. 2003
  35. pmc Lipid raft/caveolae signaling is required for Cryptococcus neoformans invasion into human brain microvascular endothelial cells
    Min Long
    Department of Microbiology, Southern Medical University, Guangzhou, China
    J Biomed Sci 19:19. 2012
    ..However, the molecular mechanism of the interaction between Cryptococcus neoformans and HBMEC, relevant to its brain invasion, is still largely unknown...
  36. ncbi request reprint Blood-brain barrier drug discovery for central nervous system infections
    Ambrose Jong
    Mailstop 57, Childrens Hospital of Los Angeles, 4650 Sunset Blvd, Los Angeles, CA 90027, USA
    Curr Drug Targets Infect Disord 5:65-72. 2005
    ..This review focuses on the major BBB drug targeting strategies in the development of therapeutics for CNS infections. A combination of these strategies will ultimately lead to improved treatments...
  37. ncbi request reprint Binding of Candida albicans enolase to plasmin(ogen) results in enhanced invasion of human brain microvascular endothelial cells
    Ambrose Y Jong
    Division of Hematology Oncology, Mailstop 57, Childrens Hospital Los Angeles, Los Angeles, CA 90027, USA
    J Med Microbiol 52:615-22. 2003
    ..The results given here indicate that Candida enolase is a plasminogen- and plasmin-binding protein and that the interaction of C. albicans enolase with the plasminogen system may contribute to invasion of the tissue barrier...
  38. pmc Inequalities and duality in gene coexpression networks of HIV-1 infection revealed by the combination of the double-connectivity approach and the Gini's method
    Chuang Ma
    Saban Research Institute of Childrens Hospital Los Angeles, Department of Pediatrics, University of Southern California, Los Angeles, CA 90027, USA
    J Biomed Biotechnol 2011:926407. 2011
    ..Understanding of the SPD problems of HIV-1 infection may provide novel intervention strategies for AIDS...
  39. pmc Lactobacillus rhamnosus GG Suppresses Meningitic E. coli K1 Penetration across Human Intestinal Epithelial Cells In Vitro and Protects Neonatal Rats against Experimental Hematogenous Meningitis
    Sheng He Huang
    Department of Pediatrics, The Saban Research Institute of Childrens Hospital Los Angeles, University of Southern California, Los Angeles, CA 90027, USA
    Int J Microbiol 2009:647862. 2009
    ..In conclusion, probiotic LGG strongly suppresses meningitic E. coli pathogens in vitro and in vivo. The results support the use of probiotic strains such as LGG for prophylaxis of neonatal sepsis and meningitis...
  40. doi request reprint Cdc6 knockdown inhibits human neuroblastoma cell proliferation
    Luo Feng
    Division of Hematology and Oncology, Childrens Hospital Los Angeles, University of Southern California Keck School of Medicine, Los Angeles, CA 90027, USA
    Mol Cell Biochem 311:189-97. 2008
    ..Our study indicates that human Cdc6 functions in several pathways to control the cell proliferation and the cell death...
  41. ncbi request reprint ibeA, a virulence factor of avian pathogenic Escherichia coli
    Pierre Germon
    INRA Centre de Tours, UR 86, Pathologie Bactérienne, 37380 Nouzilly, France
    Microbiology 151:1179-86. 2005
    ..Altogether, these results suggest a role of ibeA in the pathogenicity of some APEC strains and confirm the close relationship between APEC and other human extraintestinal pathogenic E. coli isolates...

Research Grants6

  1. Effects of Probiotics on Neonatal Bacterial Meningitis
    Sheng He Huang; Fiscal Year: 2007
    ..f8). The overall aims of this proposal are to examine whether NBM can be prevented by probiotics which have beneficial effects on human health and may overcome the crisis in antibiotic resistance due to widespread use of antibiotics. ..
  2. ESCHERICHIA COLI INVASION OF BRAIN ENDOTHELIAL CELLS
    Sheng He Huang; Fiscal Year: 2006
    ..coil K1 invasion of human BMEC. (3). To determine how E. coil K1 invasion gene expression is regulated by the ibeA locus (GimA) including IbeR and environmental signals (glucose and oxygen tension). ..
  3. ESCHERICHIA COLI INVASION OF BRAIN ENDOTHELIAL CELLS
    Sheng He Huang; Fiscal Year: 2007
    ..To further characterize the role of IbeABP in E. coil K1 invasion of human BMEC. (3). To determine how E. coil K1 invasion gene expression is regulated by the IbeR and environmental signals (glucose and oxygen tension). ..