Yilin Hu


Affiliation: University of California
Country: USA


  1. Rebelein J, Lee C, Newcomb M, Hu Y, Ribbe M. Characterization of an M-Cluster-Substituted Nitrogenase VFe Protein. MBio. 2018;9: pubmed publisher
  2. Sickerman N, Rettberg L, Lee C, Hu Y, Ribbe M. Cluster assembly in nitrogenase. Essays Biochem. 2017;61:271-279 pubmed publisher
    ..Finally, matured M-cluster in NifEN is directly transferred to NifDK, where a conformational change locks the cofactor in place. Mechanistic insights into these fascinating biosynthetic processes are detailed in this chapter. ..
  3. Jasniewski A, Hu Y, Ribbe M. Electron Paramagnetic Resonance Spectroscopy of Metalloproteins. Methods Mol Biol. 2019;1876:197-211 pubmed publisher
    ..Here, a basic introduction to the theory of EPR and the instrumentation is presented along with procedures for obtaining EPR spectra of sensitive metalloprotein species. ..
  4. Sickerman N, Hu Y, Ribbe M. Nitrogenases. Methods Mol Biol. 2019;1876:3-24 pubmed publisher
    ..This chapter discusses the structure and function of nitrogenase, covers methods that have proven useful in the elucidation of enzyme properties, and provides an overview of the three known nitrogenase variants. ..
  5. Sickerman N, Hu Y. Hydrogenases. Methods Mol Biol. 2019;1876:65-88 pubmed publisher
    ..A wide array of biochemical and spectroscopic methods has been used to elucidate hydrogenases, and this along with a general description of the main enzyme types and catalytic mechanisms is discussed in this chapter. ..
  6. Lee C, Ribbe M, Hu Y. Purification of Nitrogenase Proteins. Methods Mol Biol. 2019;1876:111-124 pubmed publisher
    ..In addition, purification and Fe-S reconstitution strategies are also outlined for the heterologously expressed nitrogenase proteins in Escherichia coli. ..
  7. Stiebritz M, Hu Y. Computational Methods for Modeling Metalloproteins. Methods Mol Biol. 2019;1876:245-266 pubmed publisher
    ..In this chapter, we discuss computational approaches to studying chemical reactions in metalloproteins and how to utilize the information hidden in homologous proteins. ..
  8. Rettberg L, Wilcoxen J, Lee C, Stiebritz M, Tanifuji K, Britt R, et al. Probing the coordination and function of Fe4S4 modules in nitrogenase assembly protein NifB. Nat Commun. 2018;9:2824 pubmed publisher
    ..These results establish an important framework for mechanistic explorations of NifB while highlighting the utility of a synthetic-cluster-based reconstitution approach employed herein in functional analyses of iron-sulfur (FeS) enzymes. ..
  9. Hu Y, Ribbe M. Nitrogenase and homologs. J Biol Inorg Chem. 2015;20:435-45 pubmed publisher
    ..In this article, we provide an overview of the structures and functions of nitrogenase and its homologs, which highlights the similarity and disparity of this uniquely versatile group of enzymes. ..

More Information


  1. Hu Y, Ribbe M. Biosynthesis of the iron-molybdenum cofactor of nitrogenase. J Biol Chem. 2013;288:13173-7 pubmed publisher
    ..Here, we review the recent progress in the research area of M-cluster assembly, with an emphasis on our work that provides useful insights into the mechanistic details of this process. ..
  2. Hu Y, Fay A, Lee C, Yoshizawa J, Ribbe M. Assembly of nitrogenase MoFe protein. Biochemistry. 2008;47:3973-81 pubmed publisher
  3. Tanifuji K, Lee C, Ohki Y, Tatsumi K, Hu Y, Ribbe M. Combining a Nitrogenase Scaffold and a Synthetic Compound into an Artificial Enzyme. Angew Chem Int Ed Engl. 2015;54:14022-5 pubmed publisher
    ..The successful outcome of this work provides the proof of concept and underlying principles for continued search of novel enzymatic activities based on this approach. ..
  4. Hu Y, Lee C, Ribbe M. Vanadium nitrogenase: a two-hit wonder?. Dalton Trans. 2012;41:1118-27 pubmed publisher
    ..This perspective provides a brief account of the catalytic function and structural basis of V-nitrogenase, as well as a short discussion of the theoretical and practical potentials of this unique metalloenzyme. ..
  5. Hu Y, Ribbe M. Decoding the nitrogenase mechanism: the homologue approach. Acc Chem Res. 2010;43:475-84 pubmed publisher
  6. Rebelein J, Hu Y, Ribbe M. Widening the Product Profile of Carbon Dioxide Reduction by Vanadium Nitrogenase. Chembiochem. 2015;16:1993-6 pubmed publisher
    ..These observations afford initial insights into the characteristics of this unique reaction and provide a potential template for future design of catalysts to recycle the greenhouse gas CO2 into useful products. ..
  7. Tanifuji K, Lee C, Sickerman N, Tatsumi K, Ohki Y, Hu Y, et al. Tracing the 'ninth sulfur' of the nitrogenase cofactor via a semi-synthetic approach. Nat Chem. 2018;10:568-572 pubmed publisher
    ..This work provides a semi-synthetic tool for protein reconstitution that could be widely applicable for the functional analysis of other FeS systems. ..
  8. Lee C, Sickerman N, Hu Y, Ribbe M. YedY: A Mononuclear Molybdenum Enzyme with a Redox-Active Ligand?. Chembiochem. 2016;17:453-5 pubmed publisher
    ..A recent electrochemical investigation suggests that the mononuclear molybdenum enzyme YdeY utilizes redox-active ligands during catalysis. ..
  9. Hu Y, Ribbe M. Nitrogenases-A Tale of Carbon Atom(s). Angew Chem Int Ed Engl. 2016;55:8216-26 pubmed publisher
  10. Hu Y, Yoshizawa J, Fay A, Lee C, Wiig J, Ribbe M. Catalytic activities of NifEN: implications for nitrogenase evolution and mechanism. Proc Natl Acad Sci U S A. 2009;106:16962-6 pubmed publisher
    ..More importantly, the discrepancy between the two systems may provide useful insights into nitrogenase mechanism and allow reconstruction of a fully functional nitrogenase from the "skeleton" enzyme, NifEN. ..
  11. Sickerman N, Hu Y, Ribbe M. Nitrogenase Assembly: Strategies and Procedures. Methods Enzymol. 2017;595:261-302 pubmed publisher
    ..Furthermore, protocols for nitrogenase protein purification and substrate reduction activity assays are described. These methods provide a solid framework for the assessment of nitrogenase assembly and catalysis. ..
  12. Hu Y, Ribbe M. Maturation of nitrogenase cofactor-the role of a class E radical SAM methyltransferase NifB. Curr Opin Chem Biol. 2016;31:188-94 pubmed publisher
  13. Fay A, Wiig J, Lee C, Hu Y. Identification and characterization of functional homologs of nitrogenase cofactor biosynthesis protein NifB from methanogens. Proc Natl Acad Sci U S A. 2015;112:14829-33 pubmed publisher
  14. Hu Y, Lee C, Ribbe M. Extending the carbon chain: hydrocarbon formation catalyzed by vanadium/molybdenum nitrogenases. Science. 2011;333:753-5 pubmed publisher
    ..Moreover, the comparison between the CO-reducing capacities of the two nitrogenases suggests that the identity of heterometal at the active cofactor site affects the efficiency and product distribution of this reaction...