Aaron A HoskinsSummaryAffiliation: University of Wisconsin Country: USA Publications
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Publications
The spliceosome: a flexible, reversible macromolecular machineAaron A Hoskins
Department of Biochemistry, University of Wisconsin Madison, WI 53706, USA
Trends Biochem Sci 37:179-88. 2012..These experiments are rewriting the textbooks, with a new picture emerging of a dynamic, malleable machine heavily influenced by the identity of its pre-mRNA substrate...
New insights into the spliceosome by single molecule fluorescence microscopyAaron A Hoskins
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation St, Worcester, MA 01605, USA
Curr Opin Chem Biol 15:864-70. 2011..These methods complement ensemble measurements of splicing in vivo and in vitro to facilitate kinetic dissection of pre-mRNA splicing...
Ordered and dynamic assembly of single spliceosomesAaron A Hoskins
Department of Biochemistry and Molecular Pharmacology, Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA
Science 331:1289-95. 2011..This experimental strategy should prove widely useful for mechanistic analysis of other macromolecular machines in environments approaching the complexity of living cells...
Single-molecule colocalization FRET evidence that spliceosome activation precedes stable approach of 5' splice site and branch siteDaniel J Crawford
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605
Proc Natl Acad Sci U S A 110:6783-8. 2013..Separation of the sites of chemistry until very late in the splicing pathway may be crucial for preventing splicing at incorrect sites...
