Aaron A Hoskins

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. doi request reprint Evolution and characterization of a benzylguanine-binding RNA aptamer
    J Xu
    Department of Biochemistry, U Wisconsin Madison, 433 Babcock Dr, Madison, WI 53706, USA
    Chem Commun (Camb) 52:549-52. 2016
  2. pmc Visualizing cellular machines with colocalization single molecule microscopy
    Joshua D Larson
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Dr, Madison, USA
    Chem Soc Rev 43:1189-200. 2014
  3. pmc Imaging of RNAs in live cells with spectrally diverse small molecule fluorophores
    Tucker J Carrocci
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Dr, Madison, WI 53706, USA
    Analyst 139:44-7. 2014
  4. pmc The spliceosome: a flexible, reversible macromolecular machine
    Aaron A Hoskins
    Department of Biochemistry, University of Wisconsin Madison, WI 53706, USA
    Trends Biochem Sci 37:179-88. 2012
  5. pmc New insights into the spliceosome by single molecule fluorescence microscopy
    Aaron A Hoskins
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation St, Worcester, MA 01605, USA
    Curr Opin Chem Biol 15:864-70. 2011
  6. pmc Alternative spliceosome assembly pathways revealed by single-molecule fluorescence microscopy
    Inna Shcherbakova
    Department of Biochemistry and Molecular Pharmacology, Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA
    Cell Rep 5:151-65. 2013
  7. pmc Single-molecule colocalization FRET evidence that spliceosome activation precedes stable approach of 5' splice site and branch site
    Daniel J Crawford
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 110:6783-8. 2013
  8. pmc Ordered and dynamic assembly of single spliceosomes
    Aaron A Hoskins
    Department of Biochemistry and Molecular Pharmacology, Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 331:1289-95. 2011
  9. doi request reprint Conformational dynamics of stem II of the U2 snRNA
    Margaret L Rodgers
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    RNA 22:225-36. 2016
  10. pmc Rapid isolation and single-molecule analysis of ribonucleoproteins from cell lysate by SNAP-SiMPull
    Margaret L Rodgers
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    RNA 21:1031-41. 2015

Collaborators

  • Melissa J Moore
  • Larry J Friedman
  • Jeff Gelles
  • Inna Shcherbakova
  • Margaret L Rodgers
  • J Xu
  • Eric G Anderson
  • Joshua D Larson
  • Joshua Larson
  • Tucker J Carrocci
  • Daniel J Crawford
  • Amir A Alwan
  • T J Carrocci
  • Alexander Dehaven
  • George Luo
  • Hannah M Mast
  • U Sandy Tretbar
  • Joshua Paulson
  • Matt Kirk
  • Eric A Drier
  • James F MacKay
  • William O'Brien

Detail Information

Publications12

  1. doi request reprint Evolution and characterization of a benzylguanine-binding RNA aptamer
    J Xu
    Department of Biochemistry, U Wisconsin Madison, 433 Babcock Dr, Madison, WI 53706, USA
    Chem Commun (Camb) 52:549-52. 2016
    ..We have evolved an RNA aptamer that tightly binds benzylguanine (bG), the key ligand for the protein SNAP-tag. The aptamer tightly binds bG fluorophores and can be purified from cellular RNA with bG agarose under native conditions. ..
  2. pmc Visualizing cellular machines with colocalization single molecule microscopy
    Joshua D Larson
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Dr, Madison, USA
    Chem Soc Rev 43:1189-200. 2014
    ..This review focuses on recent advances in colocalization single molecule spectroscopy and how this technique has been applied to enhance our understanding of transcription, RNA splicing, and translation. ..
  3. pmc Imaging of RNAs in live cells with spectrally diverse small molecule fluorophores
    Tucker J Carrocci
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Dr, Madison, WI 53706, USA
    Analyst 139:44-7. 2014
    ..These tags are based on the MS2 phage coat protein fused to either E. coli dihydrofolate reductase or the SNAP tag. Labeled RNAs can be visualized with fluorophores spanning the visible spectra range. ..
  4. pmc The spliceosome: a flexible, reversible macromolecular machine
    Aaron A Hoskins
    Department of Biochemistry, University of Wisconsin Madison, WI 53706, USA
    Trends Biochem Sci 37:179-88. 2012
    ..These experiments are rewriting the textbooks, with a new picture emerging of a dynamic, malleable machine heavily influenced by the identity of its pre-mRNA substrate...
  5. pmc New insights into the spliceosome by single molecule fluorescence microscopy
    Aaron A Hoskins
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation St, Worcester, MA 01605, USA
    Curr Opin Chem Biol 15:864-70. 2011
    ..These methods complement ensemble measurements of splicing in vivo and in vitro to facilitate kinetic dissection of pre-mRNA splicing...
  6. pmc Alternative spliceosome assembly pathways revealed by single-molecule fluorescence microscopy
    Inna Shcherbakova
    Department of Biochemistry and Molecular Pharmacology, Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA
    Cell Rep 5:151-65. 2013
    ..Thus, multiple pathways exist for assembling functional spliceosomes. These observations provide insight into the mechanisms of cross-intron coordination of initial spliceosome assembly...
  7. pmc Single-molecule colocalization FRET evidence that spliceosome activation precedes stable approach of 5' splice site and branch site
    Daniel J Crawford
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 110:6783-8. 2013
    ..Separation of the sites of chemistry until very late in the splicing pathway may be crucial for preventing splicing at incorrect sites...
  8. pmc Ordered and dynamic assembly of single spliceosomes
    Aaron A Hoskins
    Department of Biochemistry and Molecular Pharmacology, Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 331:1289-95. 2011
    ..This experimental strategy should prove widely useful for mechanistic analysis of other macromolecular machines in environments approaching the complexity of living cells...
  9. doi request reprint Conformational dynamics of stem II of the U2 snRNA
    Margaret L Rodgers
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    RNA 22:225-36. 2016
    ..Together these data suggest that multiple components of the spliceosome may have evolved to switch between conformations corresponding to open or closed active sites with the aid of metal and protein cofactors. ..
  10. pmc Rapid isolation and single-molecule analysis of ribonucleoproteins from cell lysate by SNAP-SiMPull
    Margaret L Rodgers
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    RNA 21:1031-41. 2015
    ..SNAP-SiMPull will likely find broad use for rapidly isolating complex cellular machines for single-molecule fluorescence colocalization experiments. ..
  11. pmc Design and construction of a multiwavelength, micromirror total internal reflectance fluorescence microscope
    Joshua Larson
    1 Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin, USA 2 Biophysics Graduate Program, University of Wisconsin Madison, Madison, Wisconsin, USA
    Nat Protoc 9:2317-28. 2014
    ..Depending on the experience level of the microscope builder, these time savings and the following protocol can enable mmTIRF construction to be completed within 2 months. ..
  12. doi request reprint Single molecule approaches for studying spliceosome assembly and catalysis
    Eric G Anderson
    Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, USA
    Methods Mol Biol 1126:217-41. 2014
    ..Finally, we describe how to tether the pre-mRNA onto the passivated slide surface and introduce the SNAP-tagged cell lysate for analysis of spliceosome assembly by single molecule fluorescence...