Aaron A Hoskins

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. pmc Visualizing cellular machines with colocalization single molecule microscopy
    Joshua D Larson
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Dr, Madison, USA
    Chem Soc Rev 43:1189-200. 2014
  2. pmc Imaging of RNAs in live cells with spectrally diverse small molecule fluorophores
    Tucker J Carrocci
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Dr, Madison, WI 53706, USA
    Analyst 139:44-7. 2014
  3. pmc The spliceosome: a flexible, reversible macromolecular machine
    Aaron A Hoskins
    Department of Biochemistry, University of Wisconsin Madison, WI 53706, USA
    Trends Biochem Sci 37:179-88. 2012
  4. pmc New insights into the spliceosome by single molecule fluorescence microscopy
    Aaron A Hoskins
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation St, Worcester, MA 01605, USA
    Curr Opin Chem Biol 15:864-70. 2011
  5. pmc Single-molecule colocalization FRET evidence that spliceosome activation precedes stable approach of 5' splice site and branch site
    Daniel J Crawford
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 110:6783-8. 2013
  6. pmc Ordered and dynamic assembly of single spliceosomes
    Aaron A Hoskins
    Department of Biochemistry and Molecular Pharmacology, Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 331:1289-95. 2011
  7. pmc Alternative spliceosome assembly pathways revealed by single-molecule fluorescence microscopy
    Inna Shcherbakova
    Department of Biochemistry and Molecular Pharmacology, Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA
    Cell Rep 5:151-65. 2013
  8. doi request reprint Single molecule approaches for studying spliceosome assembly and catalysis
    Eric G Anderson
    Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, USA
    Methods Mol Biol 1126:217-41. 2014

Collaborators

Detail Information

Publications8

  1. pmc Visualizing cellular machines with colocalization single molecule microscopy
    Joshua D Larson
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Dr, Madison, USA
    Chem Soc Rev 43:1189-200. 2014
    ..This review focuses on recent advances in colocalization single molecule spectroscopy and how this technique has been applied to enhance our understanding of transcription, RNA splicing, and translation. ..
  2. pmc Imaging of RNAs in live cells with spectrally diverse small molecule fluorophores
    Tucker J Carrocci
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Dr, Madison, WI 53706, USA
    Analyst 139:44-7. 2014
    ..These tags are based on the MS2 phage coat protein fused to either E. coli dihydrofolate reductase or the SNAP tag. Labeled RNAs can be visualized with fluorophores spanning the visible spectra range. ..
  3. pmc The spliceosome: a flexible, reversible macromolecular machine
    Aaron A Hoskins
    Department of Biochemistry, University of Wisconsin Madison, WI 53706, USA
    Trends Biochem Sci 37:179-88. 2012
    ..These experiments are rewriting the textbooks, with a new picture emerging of a dynamic, malleable machine heavily influenced by the identity of its pre-mRNA substrate...
  4. pmc New insights into the spliceosome by single molecule fluorescence microscopy
    Aaron A Hoskins
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation St, Worcester, MA 01605, USA
    Curr Opin Chem Biol 15:864-70. 2011
    ..These methods complement ensemble measurements of splicing in vivo and in vitro to facilitate kinetic dissection of pre-mRNA splicing...
  5. pmc Single-molecule colocalization FRET evidence that spliceosome activation precedes stable approach of 5' splice site and branch site
    Daniel J Crawford
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 110:6783-8. 2013
    ..Separation of the sites of chemistry until very late in the splicing pathway may be crucial for preventing splicing at incorrect sites...
  6. pmc Ordered and dynamic assembly of single spliceosomes
    Aaron A Hoskins
    Department of Biochemistry and Molecular Pharmacology, Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 331:1289-95. 2011
    ..This experimental strategy should prove widely useful for mechanistic analysis of other macromolecular machines in environments approaching the complexity of living cells...
  7. pmc Alternative spliceosome assembly pathways revealed by single-molecule fluorescence microscopy
    Inna Shcherbakova
    Department of Biochemistry and Molecular Pharmacology, Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA
    Cell Rep 5:151-65. 2013
    ..Thus, multiple pathways exist for assembling functional spliceosomes. These observations provide insight into the mechanisms of cross-intron coordination of initial spliceosome assembly...
  8. doi request reprint Single molecule approaches for studying spliceosome assembly and catalysis
    Eric G Anderson
    Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, USA
    Methods Mol Biol 1126:217-41. 2014
    ..Finally, we describe how to tether the pre-mRNA onto the passivated slide surface and introduce the SNAP-tagged cell lysate for analysis of spliceosome assembly by single molecule fluorescence...