Research Topics
Species | D W HorohovSummaryAffiliation: University of Kentucky Country: USA Publications
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Publications
Immunosenescence of the equine immune systemD W Horohov
M H Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY 40546, USA
J Comp Pathol 142:S78-84. 2010..This paper reviews some of our recent findings on immunosenescence in horses. Recent results on the immune response of aged horses to vaccination with a novel recombinant influenza vaccine are also discussed...
Temporal regulation of cytokine mRNA expression in equine recurrent airway obstructionD W Horohov
Department of Veterinary Science, Maxwell Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546 0099, USA
Vet Immunol Immunopathol 108:237-45. 2005..Here, we report a diverse pattern of cytokine gene expression when sampling a group of affected horses over a period of time...
Adaptation and validation of a bacteria-specific enzyme-linked immunosorbent assay for determination of farm-specific Lawsonia intracellularis seroprevalence in central Kentucky ThoroughbredsA E Page
University of Kentucky Maxwell H Gluck Equine Research Center, Lexington, Kentucky, USA
Equine Vet J Suppl 43:25-31. 2011..Lawsonia intracellularis is the causative agent of equine proliferative enteropathy (EPE), a disease for which no large-scale seroprevalence studies have been conducted...
Adaptation and validation of a bacteria-specific enzyme-linked immunosorbent assay for determination of farm-specific Lawsonia intracellularis seroprevalence in central Kentucky ThoroughbredsA E Page
University of Kentucky Maxwell H Gluck Equine Research Center, Lexington, Kentucky, USA
Equine Vet J Suppl 43:25-31. 2011..Objectives: To validate and use an equine-specific enzyme-linked immunosorbent assay (ELISA) for L. intracellularis to determine the seroprevalence of L. intracellularis on numerous farms...
Advanced age in horses affects divisional history of T cells and inflammatory cytokine productionA A Adams
Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY 40546 0099, United States
Mech Ageing Dev 129:656-64. 2008..We also show that old horses have elevated levels of IL-1beta, IL-15, IL-18 and TNFalpha gene expression in peripheral blood and significant levels of TNFalpha protein in serum, all characteristics of inflamm-aging...
Relationships among inflammatory cytokines, obesity, and insulin sensitivity in the horseM M Vick
Department of Veterinary Science, Maxwell Gluck Equine Research Center, University of Kentucky, Lexington 40546, USA
J Anim Sci 85:1144-55. 2007..Furthermore, the results suggest that an interrelationship exists among obesity, inflammatory cytokines, and IS in the horse and emphasize the need for further studies to elucidate the nature of these relationships...
In vitro and in vivo modulation of the equine immune response by parapoxvirus ovisD W Horohov
Department of Veterinary Science, Maxwell H Gluck Equine Research Center, University of Kentucky, Lexington, Kentucky 40546 0099, USA
Equine Vet J 40:468-72. 2008..While immune modulators are used routinely in equine medicine, their mechanism of action is not always known...
Influenza A viruses with truncated NS1 as modified live virus vaccines: pilot studies of safety and efficacy in horsesT M Chambers
Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, USA
Equine Vet J 41:87-92. 2009....
Effect of Propionibacterium acnes-containing immunostimulant on interferon-gamma (IFNγ) production in the neonatal foalT L Sturgill
Maxwell H Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY 40546 0099, USA
Vet Immunol Immunopathol 141:124-7. 2011..The main objective of this study was to determine the effect of administering a commercial immunomostimulant EqStim® (Propionibacterium acnes) on IFNγ production measured using intracellular staining (IFNγ) and RT-PCR...
Granzyme B-mRNA expression by equine lymphokine activated killer (LAK) cells is associated with the induction of apoptosis in target cellsC Liu
Maxwell H Gluck Equine Research Center, Department of Veterinary Science, Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, KY, USA
Vet Immunol Immunopathol 143:108-15. 2011....
Foals are interferon gamma-deficient at birthC C Breathnach
Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY 40546-0099, USA
Vet Immunol Immunopathol 112:199-209. 2006..These findings suggest that foals are born with an inherent inability to mount a Th1-based cell mediated immune response which may contribute to their susceptibility to intracellular pathogens...
Characterization of the interferon gamma response to Lawsonia intracellularis using an equine proliferative enteropathy challenge (EPE) modelA E Page
University of Kentucky Maxwell Gluck Equine Research Center, Lexington, KY 40546, USA
Vet Immunol Immunopathol 143:55-65. 2011..By contrast, those weanlings that did not develop clinical disease generated a robust IFN-γ response. These results indicate IFN-γ likely plays a significant role in protection from disease caused by L. intracellularis in the equid...
Invited review: the role of caterpillars in mare reproductive loss syndrome: a model for environmental causes of abortionK J McDowell
Department of Veterinary Science, University of Kentucky, Lexington, USA
J Anim Sci 88:1379-87. 2010..Inadvertent ingestion of ETC by pregnant mares causes MRLS. Currently the only known means to prevent MRLS is to avoid exposure of horses, particularly pregnant mares, to ETC and probably most hirsute caterpillars...
Cytokine profiles of peripheral blood and airway CD4 and CD8 T lymphocytes in horses with recurrent airway obstructionC Kleiber
Department of Clinical Veterinary Medicine, University of Berne, Länggasstrasse 124, 3012 Berne, Switzerland
Vet Immunol Immunopathol 104:91-7. 2005....
A novel model for equine recurrent airway obstructionK S Bowles
Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA
Vet Immunol Immunopathol 87:385-9. 2002..These results suggest that this approach may be useful in describing the initial stages of RAO development in the horse...
Regional antibody and cellular immune responses to equine influenza virus infection, and particle mediated DNA vaccinationG Soboll
Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin, 2015 Linden Drive West, Madison, WI 53706, USA
Vet Immunol Immunopathol 94:47-62. 2003....
Altered immune responses to a heterologous protein in ponies with heavy gastrointestinal parasite burdensJ D Edmonds
Department of Pathobiological Science, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803, USA
Equine Vet J 33:658-63. 2001..The mechanisms involved may have potential deleterious effects on standard vaccine protocols of parasitised equines...
Interleukin-4 and interferon-gamma gene expression in summer pasture-associated obstructive pulmonary disease affected horsesR E Beadle
Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803, USA
Equine Vet J 34:389-94. 2002..Further research studies will be needed to determine the most appropriate treatments to use to alter the antigen-stimulated cytokine profile being expressed by SPAOPD-affected horses or to alter the effects that these cytokines produce...
Molecular cloning, sequencing, and expression of equine interleukin-6C E Swiderski
Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70808, USA
Vet Immunol Immunopathol 77:213-20. 2000..Treatment of B9 cells with an anti-IL-6 receptor antibody ablated the response to the recombinant equine IL-6...
Cloning of equine chemokines eotaxin, monocyte chemoattractant protein (MCP)-1, MCP-2 and MCP-4, mRNA expression in tissues and induction by IL-4 in dermal fibroblastsC Benarafa
Department of Pathology and Infectious Diseases, The Royal Veterinary College, Hatfield, Hertfordshire AL9 7TA, UK
Vet Immunol Immunopathol 76:283-98. 2000..We also report the early induction (2h) of equine eotaxin and MCP-4, and the up-regulation of MCP-1 by interleukin-4 in dermal fibroblasts, suggesting these chemokines might be involved in equine skin allergic diseases...
Cloning, expression and biological activity of equine interleukin (IL)-5F M Cunningham
Department of Veterinary Basic Sciences, The Royal Veterinary College, Hawkshead Lane, North Mymms, Hertfordshire AL9 7TA, UK
Vet Immunol Immunopathol 95:63-72. 2003..The anti-human and murine moAb TRFK5 does not appear to recognise the equine protein...
Quantitative measurement of equine cytokine mRNA expression by polymerase chain reaction using target-specific standard curvesC E Swiderski
Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803, USA
J Immunol Methods 222:155-69. 1999..This assay resolves at least 2-fold differences in message, is reproducible, and has a dynamic range on the order of 3 logs...
Molecular cloning and expression of equine interleukin 2E V Vandergrifft
Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70808
Vet Immunol Immunopathol 39:395-406. 1993..Specific substitutions in those regions associated with p55 and p75 binding appear to account for this species-specifity...
Safety, efficacy, and immunogenicity of a modified-live equine influenza virus vaccine in ponies after induction of exercise-induced immunosuppressionD P Lunn
Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison 53706, USA
J Am Vet Med Assoc 218:900-6. 2001....
The effect of aging on T cell responses in the horseD W Horohov
Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA
Dev Comp Immunol 26:121-8. 2002..These results indicate that the age-related decrease in the proliferative response to mitogens is not due to a failure to produce or respond to IL-2 but probably involves some other process...
Effects of age and recombinant equine somatotropin (eST) administration on immune function in female horsesP D Guirnalda
Department of Animal Science, Cook College, Rutgers--The State University of New Jersey, New Brunswick 08903, USA
J Anim Sci 79:2651-8. 2001..05). Like other species, horses exhibit similar signs of age-related declines in various immune parameters, but those of aging were not reversed with eST treatment...
Exercise alters the immune response to equine influenza virus and increases susceptibility to infectionR W Folsom
Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803, USA
Equine Vet J 33:664-9. 2001..Our results demonstrate that exercise-induced suppression of the equine immune response to influenza virus can be associated with an increased susceptibility to disease...
Report of the equine herpesvirus-1 Havermeyer Workshop, San Gimignano, Tuscany, June 2004J D Slater
Department of Veterinary Clinical Sciences, Royal Veterinary College, Hatfield, Hertfordshire AL9 7TA, UK
Vet Immunol Immunopathol 111:3-13. 2006..This report highlights the new information that was the focus of this workshop, and is intended to summarize this material and identify the critical questions in the field...
Is exercise bad for the immune system?D W Horohov
Equine Vet J 35:113-6. 2003
Molecular cloning and sequencing of equine interleukin 4E V Vandergrifft
Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70808
Vet Immunol Immunopathol 40:379-84. 1994..The cDNA was amplified from mitogen-stimulated equine peripheral blood mononuclear cells (PBMC). The cloned PCR product shares extensive homology ith IL-4 sequences from other species...
